CN110432270A - A kind of biological sterilization preparations and its application in prevention and treatment bean sprouts anthracnose - Google Patents
A kind of biological sterilization preparations and its application in prevention and treatment bean sprouts anthracnose Download PDFInfo
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- CN110432270A CN110432270A CN201910676338.1A CN201910676338A CN110432270A CN 110432270 A CN110432270 A CN 110432270A CN 201910676338 A CN201910676338 A CN 201910676338A CN 110432270 A CN110432270 A CN 110432270A
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- biological sterilization
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- 230000001954 sterilising effect Effects 0.000 title claims abstract description 51
- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 51
- 230000002265 prevention Effects 0.000 title description 4
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- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/08—Preserving with sugars
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention belongs to vegetables sterilization technology fields; disclose a kind of biological sterilization preparations DY-6 of specificity for bean sprouts anthracnose; biological sterilization preparations DY-6 includes carboxymethyl chitosan, deacetylated chitosan and sodium benzoate; wherein, the mass ratio of carboxymethyl chitosan, chitosan and sodium benzoate is 1~5:5~12:15~24.The biological sterilization preparations can significantly inhibit the mycelia growth and spore germination of bean sprouts anthrax bacteria, control suitable for the production of bean sprouts to bean sprouts anthracnose, and relative to the fungicide such as common carbendazim and Bravo on the market, with high security, the advantages of non agricultural chemical residuum, ensure that the edible safety and good condition of bean sprouts.
Description
Technical field
The invention belongs to vegetables sterilization technology fields, and in particular to a kind of biological sterilization preparations and its in prevention and treatment bean sprouts anthrax
Application in disease.
Background technique
Bean sprouts, also known as shoot vegetable, be by various cereals, beans or set the seed of class suitable moisture, temperature, air and
It is cultivated under dark environmental condition, with fresh and tender young shoot (hypocotyl or radicle or cotyledon undeployed) for food, including Huang
Bean sprouts, mung bean sprouts, black bean sprout, pea sprouting, broad bean sprout etc..Traditional bean sprouts production technology, by using control temperature, water drenching etc.
Technological means, to produce the product that hypocotyl is sturdy, tender and crisp, radicle is short.With the improvement of living standards, people are to bean sprouts
Palatability has a higher requirement, sturdy, delicate, sweet tea is crisp, the bean sprouts without radicle is more and more popular with consumers, it has also become
The vegetable that consuming public mainly buys, remarkable in economical benefits, development potentiality are huge.
However, the generation of anthracnose seriously constrains the development of bean sprouts industry in the production process of bean sprouts.Bean sprouts anthracnose is
The Major Diseases of bean sprouts budding time are a kind of fungal diseases of generality as caused by anthrax bacteria.Anthrax bacteria is a kind of allusion quotation
The high temperature and humidity venereal bacteria of type, the radicle of main harm bean sprouts, in bean sprouts, the different location of radicle can fall ill, early stage
For the big spot of water stain shape, after slowly expand and cause to rot.Due to needing large quantity of moisture when the budding of bean sprouts, anthracnose once occurs, In
In the case that temperature and humidity is suitable for, meeting large area is spread rapidly, and disease incidence seriously affects the condition and mouth of bean sprouts up to 80% or more
Sense.
The safety of bean sprout is main problem concerned by people in the production process of bean sprouts.Currently, many factories are in bean sprouts
Inhibit the generation of bean sprouts anthracnose, such as Bravo and carbendazim etc. in production process by addition chemicals.Above-mentionedization
Although the incidence of anthracnose has been contained in the use for learning drug, making bean sprouts in appearance has biggish improvement, raw in bean sprouts
Also some potential safety problems are produced in production, such as persticide residue makes tall scrawny person become " ordeal bean bud ", cause compared with
Big security risk.Therefore, it is the edible safety for ensuring bean sprouts, needs to fungicide used in traditional bean sprouts production process
It improves, is nontoxic to prepare a kind of biological sterilization preparations safe and free of toxic and side effects and can efficiently preventing and treating anthracnose
The production of bean sprouts and the more preferable development of bean sprouts industry provide technological guidance.
Summary of the invention
The biological sterilization preparations DY-6 that it is an object of that present invention to provide a kind of in the production process of bean sprouts, the biological antibiotic system
Agent DY-6 can effectively inhibit the generation of bean sprouts anthracnose, and safety with higher.
In some preferred modes, the biological sterilization preparations include carboxymethyl chitosan, chitosan and sodium benzoate.
In some preferred modes, the chitosan is deacetylated chitosan.
In some preferred modes, deacetylation >=95% of the deacetylated chitosan.
In some preferred modes, carboxymethyl chitosan in the biological sterilization preparations, chitosan and sodium benzoate
Mass ratio is 1~5:5~12:15~24.
In some preferred modes, carboxymethyl chitosan in the biological sterilization preparations, chitosan and sodium benzoate
Mass ratio is 1:8:20.
In some preferred modes, the dosage form of the biological sterilization preparations is aqua, water dispersible granules, wettable powder
Or microcapsule formulations.
Can by by biological sterilization preparations of the invention and this field allow using with acceptable filler, and/or help
Agent such as wetting agent, dispersing agent etc. is compounded to prepare the dosage form to be formed and be readily transported and save, such as wettable powder etc..Its
In, filler includes bentonite, kaolin, white carbon black, talcum powder, diatomite, starch, precipitated calcium carbonate, attapulgite etc., auxiliary agent
Including dodecyl sodium sulfate, propylene glycol, glycerine, glycerol etc..
In some preferred modes, the present invention provides above-mentioned biological sterilization preparations DY-6 in prevention and treatment bean sprouts anthracnose
Application in terms of disease.
In some preferred modes, using biological sterilization preparations DY-6 of the invention carry out bean sprout cultivation mainly include with
Lower step:
1) carboxymethyl chitosan, chitosan and sodium benzoate are compounded in proportion, the biological sterilization preparations is prepared；
2) add water to be configured to medical fluid prepared biological sterilization preparations, the raw material seed for being used to prepare bean sprouts is placed in
It in the medical fluid, after 1~3h is impregnated in stirring, is cultivated under the conditions of 24~32 DEG C, obtains bean sprouts.
Wherein, when biological sterilization preparations add water preparating liquid, the additional amount of water can be selected according to the actual situation, preferably
Every 0.1mg biocide preparation adds 10~40mL sterile water sufficiently to dissolve.
Carboxymethyl chitosan and deacetylated chitosan are common water-solubility chitosan derivatives, have broad-spectrum antiseptic
Property, there is good inhibiting effect to most Pseudomonas of Gram-negative bacteria and gram-positive bacteria, to by virus, fungi, thin
Disease caused by bacterium etc. all has effectively preventing effect.Chitosan is tasteless nontoxic, and antibiotic property is strong, and has preservation,
It can make bean sprouts that there is good mode of appearance, and unharmful substance by inhibiting anthrax bacteria to prevent and treat bean sprouts anthracnose
Residual, can directly eat, ensure that the safety of food.
Sodium benzoate is common preservative in food, can effectively prevent food and rots, extends the shelf life.Bean sprouts
Growth needs sufficiently high moisture and temperature, easily occurs to rot, and the present invention is by by preservative benzoic acid common in food
Sodium is compounded with chitosan, and prepared biological sterilization preparations can effectively prevent bean sprouts and rot, and safety with higher.
The invention has the benefit that
(1) Common Diseases and edible feature of the present invention for bean sprouts, disclose a kind of for the special of bean sprouts anthracnose
The biological sterilization preparations DY-6 of property, biological sterilization preparations DY-6 includes carboxymethyl chitosan, deacetylated chitosan and benzene first
Sour sodium, these three substances play synergy, and the biocide preparation that compounding is formed significantly inhibits bean sprouts anthrax bacteria
Mycelia growth and spore germination, the control suitable for the production of bean sprouts to bean sprouts anthracnose, and relative to common on the market
The fungicide such as carbendazim and Bravo the advantages of non agricultural chemical residuum, ensure that the edible safety of bean sprouts with high security.
(2) biological sterilization preparations DY-6 of the invention includes water soluble chitosan, is had while playing antibacterial action
Preservation can increase the condition of bean sprouts, and bean sprouts is made to have normal shape, color and intrinsic form, listless or rotten without beating,
Bean sprouts can be made to keep fresh state in 3 days of restocking.
(3) influence that the present invention grows anthrax bacteria by comparing the deacetylated chitosan of different deacetylations, hair
The deacetylated chitosan of existing deacetylation >=95% has significant inhibiting effect to anthrax bacteria, prevents and treats crops for preparation
The fungicide of anthracnose provides technical support.
(4) biological sterilization preparations DY-6 of the invention is easy to use, time saving, safe, has preferable practical application value.
Specific embodiment
Below with reference to embodiment, the content of the present invention will be explained in more detail.It should be appreciated that implementation of the invention is not limited to
In the following examples, the accommodation in any form and/or change made to the present invention fall within the scope of the present invention.
In the present invention, if without specializing, method used in the examples is technology generally in the art, all equipment
It is that the industry often uses product with raw material etc., can be bought from market.Wherein, carboxymethyl chitosan: degree of substitution: >=80%,
CAS:83512-85-0, Shanghai Mike's woods biology Co., Ltd；Deacetylated chitosan: viscosity 100-200mpa.s, Shanghai wheat
Crin biology Co., Ltd；Sodium benzoate: purity > 99%, content of beary metal < 20ppm, CAS:532-32-1, work is given birth in Shanghai
Biological Co., Ltd；Carbendazim (Lan Feng): 50% wettable powder, Jiangsu Lam Fung Biological Chemical Co., Ltd；Bravo
(reaching Corning): 75% wettable powder, Syngenta (Suzhou) crop protection Co., Ltd.
The bean sprouts anthrax bacteria is passed through by Zhejiang Academy of Agricultural Science plant protection institute of microbiology in the present invention
Crop disease room help from separation and cryo-conservation on morbidity bean sprouts plant, the identified virus is colletotrichum
Colletotrichum point spore anthrax-bacilus (C.acutatum).
Embodiment 1: the influence that the deacetylated chitosan of different deacetylations grows bean sprouts anthrax bacteria
Using growth rate method, the deacetylated chitosan for measuring different deacetylations grows bean sprouts anthrax bacteria mycelia
Influence, wherein the deacetylation for trying deacetylated chitosan is respectively 54%, 66%, 88%, 90%, 95% and 97%,
1 × 10 will be prepared respectively for trying deacetylated chitosan3mg·L-1Mass concentration is added mixing in PDA culture medium and is made containing not
With the drug containing PDA plate of deacetylated chitosan.It will the cultured bean sprouts anthrax bacteria bacterial strain bacterium colony punching in PDA plate
Device beats the bacteria cake for taking diameter to be 5mm size, and mycelia is inoculated in drug containing PDA plate center down, and the PDA plate of drug containing is not
Above-mentioned PDA plate is placed in 26 DEG C of constant incubators after continuously cultivating 5d by blank control, using crossing method measurement for examination
Bacterial strain colony diameter, and record data.
Influence of the deacetylated chitosan of different deacetylations to bean sprouts anthrax bacteria spore germination is further measured, point
The deacetylated chitosan of difference for also known as taking 0.1mg, is substantially dissolved in 10mL sterile distilled water and is used for spore germination examination
It tests, bean sprouts anthrax spores liquid concentration is 107A/mL.It draws the configured medical fluid of 5mL to be added in 5mL spore suspension, equal proportion
Mixing.Added the mixed liquor of medicament be placed in 26 DEG C of thermostat water baths continuously cultivate for 24 hours, after 48h measure spore germination number, table
Middle numerical value is 3 repetition average values.
The influence that the different deacetylated chitosans of table 1 grow bean sprouts anthrax bacteria mycelia
Note: colony diameter is 3 repetitions, and a kind of colony diameter major diameter minor axis of table is average value
Influence of the different deacetylated chitosans of table 2 to bean sprouts anthrax bacteria spore germination
It can be seen that the deacetylated chitosan of different deacetylations to Anthracnose Pathogen bacterium bacterium from the data of Tables 1 and 2
Silk growth and spore germination have different influences, to mycelia growth and spore germination influence with the increase of deacetylation and
The trend increased is presented, when deacetylation >=95%, 54% is significantly higher than to the inhibition of mycelia growth and spore germination,
66%, the chitosan of 88%, 90% deacetylation, it is thus regarded that the deacetylated chitosan of deacetylation >=95% is to anthracnose
Germ has significantly more inhibitory effect.
Embodiment 2: carboxymethyl chitosan, deacetylated chitosan, sodium benzoate and three's compounding are to bean sprouts anthracnose
The influence of bacterium growth
The present embodiment determines carboxymethyl chitosan, deacetylated chitosan (deacetylation 95%), sodium benzoate, with
And carboxymethyl chitosan, deacetylated chitosan (deacetylation 95%) and sodium benzoate three compound in varing proportions after institute
The fungicide of preparation is grown to bean sprouts anthrax bacteria mycelia and the influence of spore germination, wherein A1 is carboxymethyl chitosan；A2 is
Deacetylated chitosan；A3 is sodium benzoate；A4 be carboxymethyl chitosan, deacetylated chitosan and sodium benzoate in mass ratio
It is compounded for 1:1:1；A5 is that carboxymethyl chitosan, deacetylated chitosan and sodium benzoate are 1:5:15 compounding in mass ratio
It forms；A6 is that carboxymethyl chitosan, deacetylated chitosan and sodium benzoate are that 1:8:20 is compounded in mass ratio；A7 is carboxylic
Methyl chitosan, deacetylated chitosan and sodium benzoate are that 5:12:24 is compounded in mass ratio；A8 is blank control, will
The reagent of above-mentioned experimental group is configured to 1 × 10 respectively3mg·L-1Mass concentration is added mixing in PDA culture medium and drug containing PDA is made
Plate, and anthrax bacteria inoculation and culture are carried out according to method described in embodiment 1, and measure colony diameter.
Add water to be configured to medical fluid respectively the biocide preparation of A1~A8 different disposal group and be used for spore germination test, wherein
Every 0.1mg biocide preparation adds 10mL sterile water sufficiently to dissolve.Bean sprouts anthrax spores liquid concentration is 107A/mL draws 5mL and matches
The medical fluid set is added in 5mL spore suspension, equal proportion mixing.Add the mixed liquor of medicament to be placed in 26 DEG C of thermostat water baths to connect
It is continuous culture for 24 hours, after 48h measure spore germination number.
The influence that 3 different disposal group of table grows bean sprouts anthrax bacteria mycelia
Note: colony diameter is 3 repetitions, and a kind of colony diameter major diameter minor axis of table is average value
Influence of the 4 different disposal group of table to bean sprouts anthrax bacteria spore germination
From experimental result as can be seen that carboxymethyl chitosan, deacetylated chitosan and sodium benzoate are to anthrax bacteria
Mycelia growth and spore germination all have certain inhibiting effect, wherein the suppression of carboxymethyl chitosan and deacetylated chitosan
Production is with being better than sodium benzoate.By finding after compounding three in proportion, inhibiting effect is significantly increased, it may be possible between three
Synergistic effect, so that bacteriostasis significantly increases, and carboxymethyl chitosan, deacetylated chitosan and sodium benzoate press quality
It is 1:1:1 compounding that effect than compounding for 1:5:15,1:8:20 and 5:12:24 is better than in mass ratio.
Embodiment 3: the influence that different fungicide grow bean sprouts anthrax bacteria mycelia
Using growth rate method, the influence that different biocide preparations grow bean sprouts anthrax bacteria mycelia is measured.It will be killed for examination
Bacteria preparation is configured to 1 × 10 respectively3mg·L-1、1×102mg·L-1、10mg·L-1、1mg·L-1、0.1mg·L-1Quality is dense
Degree is added mixing in PDA culture medium and drug containing tablet is made.Will in PDA plate cultured strains tested bacterium colony punch
The bacteria cake for taking diameter to be 5mm size is beaten, mycelia is inoculated in drug containing PDA plate center down, and the PDA plate of drug containing is not blank
Control.Strains tested colony diameter is measured using crossing method, and records data.Each fungicide processing is calculated with comparing
To the growth inhibition ratio of germ, inhibiting rate (%)=(processing group colony diameter-control group colony diameter)/(control group bacterium colony is straight
Diameter-bacteria cake diameter) × 100%.It is that dependent variable obtains toxicity regression side by independent variable, Probit (inhibiting rate) of concentration natural logrithm
Journey, according to equation calculation biocide preparation to the medium effective concentration (EC of pathogen50)。
Test result such as the following table 1, wherein A1: biological sterilization preparations of the present invention, the biocide preparation by carboxymethyl chitosan,
Deacetylated chitosan (deacetylation 95%) and sodium benzoate composition, wherein carboxymethyl chitosan, deacetylated chitosan
Mass ratio with sodium benzoate is 1:8:20；A2: Bravo；A3: carbendazim；A4: S-Ethyl ethylthio sulfonate；A5: blank control.After being inoculated with
Different drug containing tablets be placed in 26 DEG C of constant incubators continuously cultivate 5d after measure hyphal diameter, in table numerical value be 3 repetitions
Average value.
The influence that the different biocide preparations of table 5 grow bean sprouts anthrax bacteria mycelia
Note: colony diameter is 3 repetitions, and a kind of colony diameter major diameter minor axis of table is average value
As seen from Table 1, the mycelia growth inhibition of biological sterilization preparations of the invention to bean sprouts anthracnose
Effect is best, the equal < 20mm of colony diameter, and EC50It is worth minimum, the growth of common Bravo and carbendazim to hyphal diameter
There is certain inhibiting effect, S-Ethyl ethylthio sulfonate does not substantially influence bean sprouts anthracnose.
Embodiment 4: influence of the different fungicide to bean sprouts anthrax bacteria spore germination
With the biocide preparation type in embodiment 3, adds water to be configured to medical fluid different biocide preparations and be used for spore germination examination
It tests, wherein every 0.1mg biocide preparation adds 10mL sterile water sufficiently to dissolve.Bean sprouts anthrax spores liquid concentration is 107A/mL inhales
The configured biocide preparation of 5mL is taken to be added in 5mL spore suspension, equal proportion mixing.
Test result such as the following table 2, wherein A1: biological sterilization preparations of the invention；A2: Bravo；A3: carbendazim；A4: second
Allicin；A5: blank control, added the mixed liquor of medicament be placed in 26 DEG C of thermostat water baths continuously cultivate for 24 hours, spore is measured after 48h
Son sprouts number, and numerical value is 3 repetition average values in table.
Influence of the different biocide preparations of table 6 to bean sprouts anthrax bacteria spore germination
As seen from Table 2, the suppression of biological sterilization preparations of the invention to bean sprouts anthrax bacteria spore germination
Effect processed is best, and after rear spore germination rate is 0,48h for 24 hours although spore has sprouting, but germination rate is extremely low, common Bravo
There is certain inhibiting effect to bean sprouts anthracnose spore germination with carbendazim, S-Ethyl ethylthio sulfonate is substantially to bean sprouts anthracnose spore germination base
This is not influenced.
Embodiment 5: influence of the different biocide preparations to bean sprouts quality
With 3 biocide preparation type of embodiment, water is added to be configured to medical fluid different biocide preparations, wherein every 0.1mg sterilization system
Agent adds 10mL sterile water sufficiently to dissolve.500 full mung beans are taken to be placed in configured biocide preparation solution, after 2h is impregnated in stirring
It is placed in vernalization in 26 degrees Celsius of incubators after draining, observes bean sprouts incidence after 3d, 5d, 7d, and record bean sprout growth shape
Condition.
Test result such as the following table 3, wherein A1: biological sterilization preparations of the invention；A2: Bravo；A3: carbendazim；A4: second
Allicin；A5: blank control, numerical value is 3 repetition average values in table.
Influence (%) of the different fungicide of table 7 to bean sprouts anthrax bacteria spore germination
As seen from Table 3, biological sterilization preparations of the invention have not significant impact the germination percentage of bean sprouts,
And there is very strong inhibiting effect to the generation of anthracnose, and lasting effect is considerably better than other biocide preparations, bean sprouts after 7 days
Still without the generation of anthracnose, the organoleptic indicator of bean sprouts is high-quality.
Embodiment 6: influence of the biological sterilization preparations to bean sprouts safety
Physical and chemical index and microorganism of the following method to biological sterilization preparations of the invention treated bean sprouts is respectively adopted
Index is detected:
(1) physical and chemical index
Lead is by the measurement of method as defined in GB/T5009.12；Sulphite is measured according to method as defined in GB/T5009.34；
6-benzyladenine (6-BA) is measured by the defined method of GB/T 23381；Gibberellin (acid) (GA3) is according to SNT's 0350
Defined method measurement；4-chlorophenoxyacetic acid sodium is measured according to the defined method of DBS22/001.
(2) microbiological indicator
Salmonella, Shigella and staphylococcus aureus are respectively according to GB/T4789.4, GB/T4789.5 and GB/
Method as defined in T4789.10 is examined.
The physical and chemical index and microbial indicators detected result of the biological sterilization preparations of the present invention of table 8 treated bean sprouts
As seen from Table 8, biological sterilization preparations of the invention have good sterilization to bean sprouts,
Salmonella, Shigella and staphylococcus aureus are not detected in the bean sprouts of processing, meets national standard-bean sprouts health
Standard GB22556-2008, and complied with standard using the physical and chemical index of biological sterilization preparations of the invention treated bean sprouts,
It is highly-safe, it can directly eat.
The foregoing is merely the preferred embodiments of invention, are not intended to limit the invention, all in spirit and original of the invention
Within then, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (8)
1. a kind of biological sterilization preparations, which is characterized in that the biological sterilization preparations include carboxymethyl chitosan, chitosan and benzene
Sodium formate.
2. biological sterilization preparations according to claim 1, which is characterized in that the chitosan is poly- for deacetylated shell
Sugar.
3. biological sterilization preparations according to claim 2, which is characterized in that the deacetylated chitosan it is deacetylated
Degree >=95%.
4. biological sterilization preparations according to claim 1, which is characterized in that carboxymethyl chitosan in the biological sterilization preparations
The mass ratio of sugar, chitosan and sodium benzoate is 1~5:5~12:15~24.
5. biological sterilization preparations according to claim 4, which is characterized in that carboxymethyl chitosan in the biological sterilization preparations
The mass ratio of sugar, chitosan and sodium benzoate is 1:8:20.
6. biological sterilization preparations according to claim 1, which is characterized in that the dosage form of the biological sterilization preparations is water
Agent, water dispersible granules, wettable powder or microcapsule formulations.
7. application of the biological sterilization preparations as described in any one of claims 1 to 6 in terms of preventing and treating bean sprouts anthracnose disease.
8. application according to claim 7, which comprises the following steps:
1) carboxymethyl chitosan, chitosan and sodium benzoate are compounded in proportion, the biological sterilization preparations is prepared；
2) add water to be configured to medical fluid prepared biological sterilization preparations, the raw material seed for being used to prepare bean sprouts is placed in described
It in medical fluid, after 1~3h is impregnated in stirring, is cultivated under the conditions of 24~32 DEG C, obtains bean sprouts.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102119720A (en) * | 2010-12-31 | 2011-07-13 | 张胜勇 | Carrot bifunctional enzyme-containing fruit and vegetable fresh-keeping agent |
| CN105413650A (en) * | 2016-01-07 | 2016-03-23 | 苏州法斯特信息科技有限公司 | Rectorite wastewater adsorption material and preparation method thereof |
| CN108244229A (en) * | 2018-02-12 | 2018-07-06 | 柳州晟聚农业科技有限公司 | A kind of fruit antistaling agent and preparation method thereof |
-
2019
- 2019-07-25 CN CN201910676338.1A patent/CN110432270A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102119720A (en) * | 2010-12-31 | 2011-07-13 | 张胜勇 | Carrot bifunctional enzyme-containing fruit and vegetable fresh-keeping agent |
| CN105413650A (en) * | 2016-01-07 | 2016-03-23 | 苏州法斯特信息科技有限公司 | Rectorite wastewater adsorption material and preparation method thereof |
| CN108244229A (en) * | 2018-02-12 | 2018-07-06 | 柳州晟聚农业科技有限公司 | A kind of fruit antistaling agent and preparation method thereof |
Non-Patent Citations (6)
| Title |
|---|
| A. B. AL ZAEMEY等: ""Studies on the effect of fruit-coating polymers and organic acids on growth of Colletotrichum musae in vitro and on postharvest control of anthracnose of bananas"", 《MYCOL. RES.》 * |
| 于新 主编: "《天然食品添加剂》", 31 March 2014, 中国轻工业出版社 * |
| 王富华等编著: "《蔬菜生产安全控制技术》", 31 December 2008, 广东省出版集团 广东科技出版社 * |
| 苏云 等: ""比浊法羧甲基壳聚糖抑菌性能的研究"", 《滁州学院学报》 * |
| 苏云 等: ""羧甲基壳聚糖的制备及抑菌性能的研究"", 《滁州学院学报》 * |
| 邓舜扬编: "《食品保鲜技术》", 31 January 2006, 中国轻工业出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111202089A (en) * | 2020-01-19 | 2020-05-29 | 浙江省农业科学院 | Disinfectant for bean sprout production and application thereof |
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