CN102119720A - Carrot bifunctional enzyme-containing fruit and vegetable fresh-keeping agent - Google Patents
Carrot bifunctional enzyme-containing fruit and vegetable fresh-keeping agent Download PDFInfo
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- CN102119720A CN102119720A CN 201010615541 CN201010615541A CN102119720A CN 102119720 A CN102119720 A CN 102119720A CN 201010615541 CN201010615541 CN 201010615541 CN 201010615541 A CN201010615541 A CN 201010615541A CN 102119720 A CN102119720 A CN 102119720A
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- fruit
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- bifunctional enzyme
- radish
- keeping
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Abstract
The invention relates to a carrot bifunctional enzyme-containing fruit and vegetable fresh-keeping agent which comprises the components with content: 40-80mg of carrot bifunctional enzyme, 1-10mg of polysaccharide, 0.01-1mg of preservative, 0.1-5mg of surface active agent, 0.01-1mg of antioxidant, 1-20ml of edible ethanol and 50-100ml of water. The fresh-keeping agent is capable of effectively killing fungus leading fruits and vegetables to decay and preventing water loss, and is simple in formulation and good in fresh-keeping and green-keeping effects.
Description
Technical field
The present invention relates to the antistaling agent field, particularly, relate to a kind of fruit and vegetable fresh-keeping agent that contains the radish bifunctional enzyme.
Background technology
Fruit and vegetable is the necessity during people live, but because of it has stronger seasonality and region, solve the insurance green-keeping problem in storage of vegetables and fruit and the transportation, thereby, be the heat subject of studying both at home and abroad at present for people provide fresh fruits and vegetables.
Epidermis of fruits and vegetables and epidermis are covered with one deck wax shape material outward, and this material has the effect that prevents that microorganism from invading, and therefore general normal fruits and vegetables interior tissue is aseptic.But when fruits and vegetables epidermal tissue was subjected to stabbing of insect or other mechanical damage, from then on microorganism will invade and breed, thereby promoted more easy damaged of rotting of fruits and vegetables, the especially fruits and vegetables that maturity is high.In addition, the material compositing characteristic of fruit and vegetables is based on carbohydrate and water, the moisture height, and these are that fruits and vegetables cause the key factor (fruit 85%, vegetables 88%) that microorganism is rotten easily; Next fruit pH＜4.5, between vegetables pH5～7, this has determined can carry out in the fruit and vegetable monoid of the microorganism of growth and breeding.Cause the microorganism that fruit is rotten, beginning can only be saccharomycete, mould; Cause that the rotten microorganism of vegetables is mould, saccharomycete and minority bacterium.Modal phenomenon is that at first mould breeds in the breeding of fruits and vegetables epidermis injury place or in the zone that fruit and vegetable surfaces has pollutant to adhere to, after invading the fruits and vegetables tissue, the cellulose of tissue wall is at first destroyed, and then decompose pectin, protein, starch, organic acid, carbohydrate, saccharomycete and bacterium begin breeding then.Because microbial reproduction, fruits and vegetables just show dark spot in appearance, and tissue becomes soft, send out continuous, depression, distortion, and become the slurries shape gradually or even water aqueous, and produced various taste, as tart flavour, fragranced, vinosity etc.
At present, an important method of domestic preserving fruit and vegetable utilizing is coating-film fresh-keeping, low temperature storage and uses antistaling agent that these methods have all obtained significant effect.
Yet, in recent years,, demand studying environmental protection, nontoxic, pollution-free, healthy fresh preserving agent for fruit and vegetable urgently along with the enhancing of people's food security consciousness and the frequent generation of food security incident.
The existing radish bifunctional enzyme of discovering is to extract the natural plant that obtains from radish, has chitinase activity and lysozyme activity concurrently, not only can kill gramnegative bacterium, also can kill gram-positive bacterium.In the plant pest that causes because of microorganism of antagonism, have key properties such as environmental protection, nontoxic, pollution-free, health, yet, at present, the fruit and vegetable fresh-keeping agent based on the radish bifunctional protein does not also appear in the world wide.
Summary of the invention
For this reason, the application provides a kind of fruit and vegetable fresh-keeping agent that contains the radish bifunctional enzyme, and this antistaling agent can effectively be killed the fungi that causes fruits and vegetables to rot, and prevents the loss of moisture, and its prescription is simple, and fresh-keeping guarantor is green effective.
A kind of fruit and vegetable fresh-keeping agent that contains the radish bifunctional enzyme, contained component and each components contents are as follows:
Radish bifunctional enzyme 40-80mg;
Polysaccharide 1-10mg;
Anticorrisive agent 0.01-1mg;
Surfactant 0.1-5mg;
Antioxidant 0.01-1mg;
Edible ethanol 1-20ml;
Water 50-100ml;
Described polysaccharide is selected from shitosan, CMC, the composition of one or more in carboxymethyl cellulose, the pectin.
Described anticorrisive agent is selected from one or more the composition in benzoic acid, Sodium Benzoate, sorbic acid, the potassium sorbate.
The described activating agent that shows is selected from Tween-20, Tween-60 or its mixture.
Described antioxygen base is selected from ascorbic acid, TBHQ or its composition.
The fruit and vegetable fresh-keeping agent that contains the radish bifunctional enzyme of the present invention during use, soaks 50-300s with fruits and vegetables at the described fruit and vegetable fresh-keeping agent that contains the radish bifunctional enzyme, takes out, and dries the back storage naturally and gets final product.
The fruit and vegetable fresh-keeping agent that contains the radish bifunctional enzyme of the present invention, it contains contains the radish bifunctional enzyme and promptly can kill gramnegative bacterium, the gram-positive bacterium of can going out less again, thus prevent that fruits and vegetables from corroding rotting of causing because of bacterium.In addition, described fruit and vegetable fresh-keeping agent contains anticorrisive agent, antioxidant and synergist, has effectively prevented the oxidation of nutritional labeling in the fruits and vegetables, has preserved moisture, thereby prevents that further fruits and vegetables from corroding rotting of causing because of bacterium.
The fruit and vegetable fresh-keeping agent that contains the radish bifunctional enzyme of the present invention, its prescription is simple, can effectively kill the fungi that causes fruits and vegetables to rot, and prevents the loss of moisture, and fresh-keeping guarantor is green effective.
The specific embodiment
Embodiment 1:
A kind of fruit and vegetable fresh-keeping agent that contains the radish bifunctional enzyme, contained component and each components contents are as follows:
Radish bifunctional enzyme 40mg;
Polysaccharide 5mg;
Anticorrisive agent 1mg;
Surfactant 2.5mg;
Antioxidant 0.5mg;
Edible ethanol 10ml;
Water 80ml;
Described polysaccharide is the mixture of shitosan and carboxymethyl cellulose; Described anticorrisive agent is a Sodium Benzoate; The described activating agent that shows is a Tween-20; Described antioxygen base is selected from ascorbic acid.
Above-mentioned antistaling agent is used for the fresh-keeping of Fuji apple red apple, the Fuji apple red apple is immersed in the above-mentioned fruit and vegetable fresh-keeping agent 3 minutes, take out, drain, packing was deposited three months in the humidity of 15-25 ℃ of room temperature and 70-95%, good fruit rate reaches 97%, moisture loss reaches 4%, and control group fruit rate well is 80%, and moisture loss is 12%.
Embodiment 2:
A kind of fruit and vegetable fresh-keeping agent that contains the radish bifunctional enzyme, contained component and each components contents are as follows:
Radish bifunctional enzyme 80mg;
Polysaccharide 1mg;
Anticorrisive agent 0.5mg;
Surfactant 5mg;
Antioxidant 1mg;
Edible ethanol 20ml;
Water 100ml;
Described polysaccharide is the mixture of CMC and pectin; Described anticorrisive agent is benzoic acid and potassium sorbate; The described activating agent that shows is a Tween-60; Described antioxygen base is selected from TBHQ.
Above-mentioned antistaling agent is used for the fresh-keeping of Fuji apple red apple, the Fuji apple red apple is immersed in the above-mentioned fruit and vegetable fresh-keeping agent 3 minutes, take out, drain, packing was deposited three months in the humidity of 15-25 ℃ of room temperature and 70-95%, good fruit rate reaches 98%, moisture loss reaches 3.8%, and control group fruit rate well is 80%, and moisture loss is 12%.
Embodiment 3:
A kind of fruit and vegetable fresh-keeping agent that contains the radish bifunctional enzyme, contained component and each components contents are as follows:
Radish bifunctional enzyme 60mg;
Polysaccharide 10mg;
Anticorrisive agent 0.01mg;
Surfactant 0.1mg;
Antioxidant 0.01mg;
Edible ethanol 1ml;
Water 50ml;
Described polysaccharide is a CMC; Described anticorrisive agent is a benzoic acid; The described activating agent that shows is a Tween-60; Described antioxygen base is selected from TBHQ.
Above-mentioned antistaling agent is used for the fresh-keeping of Fuji apple red apple, the Fuji apple red apple is immersed in the above-mentioned fruit and vegetable fresh-keeping agent 3 minutes, take out, drain, packing was deposited three months in the humidity of 15-25 ℃ of room temperature and 70-95%, good fruit rate reaches 96%, moisture loss reaches 3.5%, and control group fruit rate well is 80%, and moisture loss is 12%.
Above content be in conjunction with concrete preferred implementation to further describing that the present invention did, can not assert that concrete enforcement of the present invention is confined to these explanations.For the general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, its framework form can be flexible and changeable, can the subseries product.Just make some simple deduction or replace, all should be considered as belonging to the scope of patent protection that the present invention is determined by claims of being submitted to.
Claims (5)
1. a fruit and vegetable fresh-keeping agent that contains the radish bifunctional enzyme is characterized in that, contained component and each components contents are as follows:
Radish bifunctional enzyme 40-80mg;
Polysaccharide 1-10mg;
Anticorrisive agent 0.01-1mg;
Surfactant 0.1-5mg;
Antioxidant 0.01-1mg;
Edible ethanol 1-20ml;
Water 50-100ml.
2. the fruit and vegetable fresh-keeping agent that contains the radish bifunctional enzyme as claimed in claim 1 is characterized in that described polysaccharide is selected from shitosan, CMC, the composition of one or more in carboxymethyl cellulose, the pectin.
3. the fruit and vegetable fresh-keeping agent that contains the radish bifunctional enzyme as claimed in claim 1 is characterized in that, described anticorrisive agent is selected from one or more the composition in benzoic acid, Sodium Benzoate, sorbic acid, the potassium sorbate.
4. the fruit and vegetable fresh-keeping agent that contains the radish bifunctional enzyme as claimed in claim 1 is characterized in that, the described activating agent that shows is selected from Tween-20, Tween-60 or its mixture.
5. the fruit and vegetable fresh-keeping agent that contains the radish bifunctional enzyme as claimed in claim 1 is characterized in that, described antioxygen base is selected from ascorbic acid, TBHQ or its composition.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010615541 CN102119720A (en) | 2010-12-31 | 2010-12-31 | Carrot bifunctional enzyme-containing fruit and vegetable fresh-keeping agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010615541 CN102119720A (en) | 2010-12-31 | 2010-12-31 | Carrot bifunctional enzyme-containing fruit and vegetable fresh-keeping agent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102119720A true CN102119720A (en) | 2011-07-13 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010615541 Pending CN102119720A (en) | 2010-12-31 | 2010-12-31 | Carrot bifunctional enzyme-containing fruit and vegetable fresh-keeping agent |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105794962A (en) * | 2016-04-14 | 2016-07-27 | 华中农业大学 | Fresh-cut fruit and vegetable color-protecting and freshness-retaining method |
| CN109156520A (en) * | 2018-10-24 | 2019-01-08 | 淮阴师范学院 | A kind of green composite preservative of Green leaf vegetable anti-corrosion guarantor |
| CN110432270A (en) * | 2019-07-25 | 2019-11-12 | 浙江省农业科学院 | A kind of biological sterilization preparations and its application in prevention and treatment bean sprouts anthracnose |
| CN113812459A (en) * | 2021-09-17 | 2021-12-21 | 广州大学 | Chitosan-pectin-salicylic acid composite membrane for preventing and treating cold damage of guava and preparation method thereof |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1993011671A2 (en) * | 1991-12-10 | 1993-06-24 | Genencor International, Inc. | Fungistatic method and composition employing type ii endoglycosidases and/or peroxidases and/or chitinases |
| CN1379088A (en) * | 2002-04-27 | 2002-11-13 | 陈燕娜 | Radish chitin bindin and its preparing process |
| CN1466883A (en) * | 2002-07-12 | 2004-01-14 | 上海三瑞化学有限公司 | Bi-component edible fruit and vegetable coating fresh-keeping agent and uses thereof |
| CN1538813A (en) * | 2001-07-13 | 2004-10-20 | Composition having bacteristatic and bactericidal activity against bacterial spores and vegetative cells and process for treating foods therewith | |
| CN101112209A (en) * | 2006-07-28 | 2008-01-30 | 国家农产品保鲜工程技术研究中心（天津） | Fruit and vegetables corrosion protection antistaling agent containing regrouped chitinase |
| CN101189981A (en) * | 2006-11-28 | 2008-06-04 | 国家农产品保鲜工程技术研究中心（天津） | Composite antistaling agent for fruits and vegetables |
| CN101406435A (en) * | 2008-11-18 | 2009-04-15 | 宋秋兰 | Use of radish chitin-binding protein in preparing dental health product |
| CN101434936A (en) * | 2008-12-10 | 2009-05-20 | 宋秋兰 | Industrial preparation of radish dual-function enzyme with chitinase and antalzyme activity |
| CN101535041A (en) * | 2006-09-18 | 2009-09-16 | 俄勒冈州，由高等教育州委员会代表俄勒冈州立大学 | Lysozyme-chitosan films |
| ES2334741A1 (en) * | 2008-06-26 | 2010-03-15 | Consejo Superior De Investigaciones Cientificas (Csic) | Cryoprotecting protein with quitinase activity at low temperatures, procedure for its obtaining and its applications (Machine-translation by Google Translate, not legally binding) |
| CN101732747A (en) * | 2008-11-25 | 2010-06-16 | 宋秋兰 | Application of radish chitin-binding proteins in preparing air freshener |
-
2010
- 2010-12-31 CN CN 201010615541 patent/CN102119720A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1993011671A2 (en) * | 1991-12-10 | 1993-06-24 | Genencor International, Inc. | Fungistatic method and composition employing type ii endoglycosidases and/or peroxidases and/or chitinases |
| CN1538813A (en) * | 2001-07-13 | 2004-10-20 | Composition having bacteristatic and bactericidal activity against bacterial spores and vegetative cells and process for treating foods therewith | |
| CN1379088A (en) * | 2002-04-27 | 2002-11-13 | 陈燕娜 | Radish chitin bindin and its preparing process |
| CN1466883A (en) * | 2002-07-12 | 2004-01-14 | 上海三瑞化学有限公司 | Bi-component edible fruit and vegetable coating fresh-keeping agent and uses thereof |
| CN101112209A (en) * | 2006-07-28 | 2008-01-30 | 国家农产品保鲜工程技术研究中心（天津） | Fruit and vegetables corrosion protection antistaling agent containing regrouped chitinase |
| CN101535041A (en) * | 2006-09-18 | 2009-09-16 | 俄勒冈州，由高等教育州委员会代表俄勒冈州立大学 | Lysozyme-chitosan films |
| CN101189981A (en) * | 2006-11-28 | 2008-06-04 | 国家农产品保鲜工程技术研究中心（天津） | Composite antistaling agent for fruits and vegetables |
| ES2334741A1 (en) * | 2008-06-26 | 2010-03-15 | Consejo Superior De Investigaciones Cientificas (Csic) | Cryoprotecting protein with quitinase activity at low temperatures, procedure for its obtaining and its applications (Machine-translation by Google Translate, not legally binding) |
| CN101406435A (en) * | 2008-11-18 | 2009-04-15 | 宋秋兰 | Use of radish chitin-binding protein in preparing dental health product |
| CN101732747A (en) * | 2008-11-25 | 2010-06-16 | 宋秋兰 | Application of radish chitin-binding proteins in preparing air freshener |
| CN101434936A (en) * | 2008-12-10 | 2009-05-20 | 宋秋兰 | Industrial preparation of radish dual-function enzyme with chitinase and antalzyme activity |
Non-Patent Citations (3)
| Title |
|---|
| 《JOURNAL OF FERMENTATION AND BIOENINEERING》 19951231 YOSHINARI YAMAMOTO等 Purification and Characteristics of Chitinase Secreted by Cultured Wasabia japonica Cells 第148-152页 1-6 第80卷, 第2期 * |
| 《乳业科学与技术》 20061101 谷绒等 溶菌酶在食品工业中的应用 第264-266页 1-6 , 第6期 * |
| 《华南农业大学学报》 19970430 高向阳等 萝卜溶菌酶抑菌效果的初步研究 第72-75页 1-6 , 第2期 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105794962A (en) * | 2016-04-14 | 2016-07-27 | 华中农业大学 | Fresh-cut fruit and vegetable color-protecting and freshness-retaining method |
| CN109156520A (en) * | 2018-10-24 | 2019-01-08 | 淮阴师范学院 | A kind of green composite preservative of Green leaf vegetable anti-corrosion guarantor |
| CN110432270A (en) * | 2019-07-25 | 2019-11-12 | 浙江省农业科学院 | A kind of biological sterilization preparations and its application in prevention and treatment bean sprouts anthracnose |
| CN113812459A (en) * | 2021-09-17 | 2021-12-21 | 广州大学 | Chitosan-pectin-salicylic acid composite membrane for preventing and treating cold damage of guava and preparation method thereof |
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Application publication date: 20110713 |