CN110396133B - 一种以白介素12为活性成分的融合蛋白型药物前体 - Google Patents

一种以白介素12为活性成分的融合蛋白型药物前体 Download PDF

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CN110396133B
CN110396133B CN201810376920.1A CN201810376920A CN110396133B CN 110396133 B CN110396133 B CN 110396133B CN 201810376920 A CN201810376920 A CN 201810376920A CN 110396133 B CN110396133 B CN 110396133B
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CN110396133A (zh
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傅阳心
彭华
薛娣媛
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Shanghai Kangkang Biotechnology Co.,Ltd.
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Immune Targeting Inc
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Priority to KR1020207033158A priority patent/KR20210024446A/ko
Priority to AU2019261411A priority patent/AU2019261411B2/en
Priority to CA3097995A priority patent/CA3097995A1/en
Priority to BR112020021791-3A priority patent/BR112020021791A2/pt
Priority to JP2020559408A priority patent/JP2021521822A/ja
Priority to US17/049,021 priority patent/US12473337B2/en
Priority to EA202092459A priority patent/EA202092459A1/ru
Priority to CN201980042842.4A priority patent/CN112638938A/zh
Priority to EP19792585.2A priority patent/EP3810639A4/en
Priority to PCT/US2019/028933 priority patent/WO2019209965A2/en
Publication of CN110396133A publication Critical patent/CN110396133A/zh
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/5434—IL-12
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C07K14/7155—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for interleukins [IL]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Epidemiology (AREA)
  • Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
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  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
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CN201810376920.1A 2018-04-25 2018-04-25 一种以白介素12为活性成分的融合蛋白型药物前体 Active CN110396133B (zh)

Priority Applications (12)

Application Number Priority Date Filing Date Title
CN201810376920.1A CN110396133B (zh) 2018-04-25 2018-04-25 一种以白介素12为活性成分的融合蛋白型药物前体
CN201980042842.4A CN112638938A (zh) 2018-04-25 2019-04-24 白介素12融合蛋白及其组合物和治疗方法
CA3097995A CA3097995A1 (en) 2018-04-25 2019-04-24 Interleukin 12 fusion proteins, and compositions and therapeutic methods thereof
BR112020021791-3A BR112020021791A2 (pt) 2018-04-25 2019-04-24 proteínas de fusão de interleucina 12 e composições e métodos terapêuticos das mesmas
JP2020559408A JP2021521822A (ja) 2018-04-25 2019-04-24 インターロイキン１２融合タンパク質および組成物、ならびにその治療方法
US17/049,021 US12473337B2 (en) 2018-04-25 2019-04-24 Homodimeric or heterodimeric interleukin 12 fusion proteins, and compositions and therapeutic methods thereof
KR1020207033158A KR20210024446A (ko) 2018-04-25 2019-04-24 인터루킨 12 융합 단백질 및 이의 조성물 및 치료 방법
AU2019261411A AU2019261411B2 (en) 2018-04-25 2019-04-24 Interleukin 12 fusion proteins, and compositions and therapeutic methods thereof
EP19792585.2A EP3810639A4 (en) 2019-04-24 Interleukin 12 fusion proteins, and compositions and therapeutic methods thereof
PCT/US2019/028933 WO2019209965A2 (en) 2018-04-25 2019-04-24 Interleukin 12 fusion proteins, and compositions and therapeutic methods thereof
EA202092459A EA202092459A1 (ru) 2018-04-25 2019-04-24 Гибридные белки интерлейкина 12, композиции и способы терапевтического применения, которые их включают
JP2024197045A JP2025032113A (ja) 2018-04-25 2024-11-11 インターロイキン１２融合タンパク質および組成物、ならびにその治療方法

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CN201810376920.1A CN110396133B (zh) 2018-04-25 2018-04-25 一种以白介素12为活性成分的融合蛋白型药物前体

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CN110396133A CN110396133A (zh) 2019-11-01
CN110396133B true CN110396133B (zh) 2021-07-23

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CN201810376920.1A Active CN110396133B (zh) 2018-04-25 2018-04-25 一种以白介素12为活性成分的融合蛋白型药物前体
CN201980042842.4A Pending CN112638938A (zh) 2018-04-25 2019-04-24 白介素12融合蛋白及其组合物和治疗方法

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US (1) US12473337B2 (https=)
JP (2) JP2021521822A (https=)
KR (1) KR20210024446A (https=)
CN (2) CN110396133B (https=)
AU (1) AU2019261411B2 (https=)
BR (1) BR112020021791A2 (https=)
CA (1) CA3097995A1 (https=)
EA (1) EA202092459A1 (https=)
WO (1) WO2019209965A2 (https=)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20210017247A1 (en) 2017-07-03 2021-01-21 Torque Therapeutics, Inc. Fusion Molecules Targeting Immune Regulatory Cells and Uses Thereof
US12459980B2 (en) 2018-07-25 2025-11-04 AskGene Pharma, Inc. IL-21 prodrugs and methods of use thereof
CN113710229B (zh) 2019-02-25 2026-01-06 芝加哥大学 用与抗炎剂连接的ecm亲和肽治疗炎症性和自身免疫性病症的方法和组合物
KR20220020879A (ko) 2019-06-12 2022-02-21 에스크진 파마, 아이엔씨. 새로운 il-15 프로드럭 및 이를 사용하는 방법
MX2022000991A (es) * 2019-07-25 2022-05-24 Univ Chicago Composiciones y metodos que comprenden agentes terapeuticos activados por proteasa.
WO2021142471A1 (en) 2020-01-11 2021-07-15 AskGene Pharma, Inc. Novel masked cytokines and methods of use thereof
AU2021248919A1 (en) * 2020-04-01 2022-10-13 Xilio Development, Inc. Masked IL-12 cytokines and their cleavage products
WO2022115865A2 (en) * 2020-11-25 2022-06-02 Xilio Development, Inc. Tumor-specific cleavable linkers
WO2022129313A1 (en) * 2020-12-18 2022-06-23 F. Hoffmann-La Roche Ag Precursor proteins and kit for targeted therapy
IL306074A (en) * 2021-03-24 2023-11-01 Alkermes Inc UPAR antibodies and fusion proteins with them
CN117396229A (zh) * 2021-04-21 2024-01-12 Cue生物制药股份有限公司 Mhc ii类t细胞调节多肽及其使用方法
TW202321282A (zh) * 2021-07-19 2023-06-01 美商再生元醫藥公司 Il12受體促效劑及其使用方法
JP2024539139A (ja) 2021-10-20 2024-10-28 シンセカイン インコーポレイテッド ヘテロ二量体ｆｃサイトカインおよびその使用
EP4649088A1 (en) * 2023-01-13 2025-11-19 Regeneron Pharmaceuticals, Inc. Il12 receptor agonists and methods of use thereof
CN121263432A (zh) 2023-04-12 2026-01-02 上海康抗生物技术有限公司 包含掩蔽型白介素12的多功能分子及使用方法
WO2025119304A1 (en) * 2023-12-07 2025-06-12 Wuxi Biologics (Shanghai) Co., Ltd. Heterodimeric proteins against il-12 receptor
WO2025256578A1 (zh) * 2024-06-12 2025-12-18 龙启生物制药(杭州)有限公司 Treg细胞靶向IL12融合蛋白及其制备、治疗方法
WO2026020161A1 (en) * 2024-07-19 2026-01-22 Cytomx Therapeutics, Inc. Activatable il-12 constructs and related compositions and methods

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1418184A1 (en) * 2002-11-08 2004-05-12 Cell Center Cologne GmbH Recombinant fusion protein consisting of the p40 and p35 subunits of IL-12 and a ScFv and use thereof
CN105218682A (zh) * 2015-10-26 2016-01-06 深圳精准医疗科技有限公司 经il-12/cd62l融合蛋白改造的肿瘤治疗剂及其制法和用途
CN105543279A (zh) * 2014-10-30 2016-05-04 常州卡斯比生物科技有限公司 一种防治辐射线损伤、肿瘤治疗的IL-12/Fc融合蛋白的制备方法及其药剂
WO2018030806A1 (ko) * 2016-08-10 2018-02-15 아주대학교산학협력단 항체 중쇄불변부위 이종이중체에 융합된 사이토카인 및 이를 포함하는 약제학적 조성물

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5116964A (en) 1989-02-23 1992-05-26 Genentech, Inc. Hybrid immunoglobulins
ATE194384T1 (de) 1989-09-12 2000-07-15 Hoffmann La Roche Tnf-bindende proteine
DE69334070T2 (de) 1992-05-26 2007-01-04 Immunex Corp., Thousand Oaks Neue zytokine die cd30 binden
AU1407997A (en) 1995-12-01 1997-06-19 Beth Israel Hospital Il-12 p40 subunit fusion polypeptides and uses thereof
JP4046354B2 (ja) 1996-03-18 2008-02-13 ボード オブ リージェンツ，ザ ユニバーシティ オブ テキサス システム 増大した半減期を有する免疫グロブリン様ドメイン
WO1998028427A1 (en) 1996-12-20 1998-07-02 Amgen Inc. Ob fusion protein compositions and methods
EP1489100B1 (en) 1997-12-08 2016-06-15 Merck Patent GmbH Heterodimeric fusion proteins useful for targeted immune therapy and general immune stimulation
ATE407697T1 (de) 1999-07-13 2008-09-15 Bolder Biotechnology Inc Erythropoietin immunglobulin fusionsproteine
JP2007531707A (ja) 2003-10-15 2007-11-08 ピーディーエル バイオファーマ， インコーポレイテッド ＩＧの重鎖定常領域の位置２５０、３１４および／または４２８の変異誘発によるＦｃ融合タンパク質血清半減期の改変
JP4718274B2 (ja) 2005-08-25 2011-07-06 東京エレクトロン株式会社 半導体製造装置，半導体製造装置の流量補正方法，プログラム
EP1969000A2 (en) 2005-12-06 2008-09-17 Centre National de la Recherche Scientifique Cell penetrating peptides for intracellular delivery of molecules
PT3107573T (pt) * 2014-02-19 2019-01-10 Merck Patent Gmbh Imunoterapia com il-12 visada ao cancro
CN104628870A (zh) * 2015-02-04 2015-05-20 中国药科大学 一种人IL12Rβ1-CHR蛋白及其Fc融合蛋白
EP3184548A1 (en) * 2015-12-23 2017-06-28 Miltenyi Biotec GmbH Chimeric antigen receptor with cytokine receptor activating or blocking domain
MX386014B (es) * 2016-08-10 2025-03-18 Univ Ajou Ind Academic Coop Found Citocina fusionada a fc heterodimérico, y composición farmacéutica que comprende la misma.

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1418184A1 (en) * 2002-11-08 2004-05-12 Cell Center Cologne GmbH Recombinant fusion protein consisting of the p40 and p35 subunits of IL-12 and a ScFv and use thereof
CN105543279A (zh) * 2014-10-30 2016-05-04 常州卡斯比生物科技有限公司 一种防治辐射线损伤、肿瘤治疗的IL-12/Fc融合蛋白的制备方法及其药剂
CN105218682A (zh) * 2015-10-26 2016-01-06 深圳精准医疗科技有限公司 经il-12/cd62l融合蛋白改造的肿瘤治疗剂及其制法和用途
WO2017071173A1 (zh) * 2015-10-26 2017-05-04 杨世成 经il-12/cd62l融合蛋白改造的肿瘤治疗剂及其制法和用途
WO2018030806A1 (ko) * 2016-08-10 2018-02-15 아주대학교산학협력단 항체 중쇄불변부위 이종이중체에 융합된 사이토카인 및 이를 포함하는 약제학적 조성물

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WO2019209965A2 (en) 2019-10-31
US20220162280A1 (en) 2022-05-26
JP2025032113A (ja) 2025-03-11
KR20210024446A (ko) 2021-03-05
WO2019209965A3 (en) 2019-12-05
CN112638938A (zh) 2021-04-09
BR112020021791A2 (pt) 2021-02-23
EA202092459A1 (ru) 2021-06-15
CN110396133A (zh) 2019-11-01
CA3097995A1 (en) 2019-10-31
JP2021521822A (ja) 2021-08-30
AU2019261411B2 (en) 2024-11-14
US12473337B2 (en) 2025-11-18
AU2019261411A1 (en) 2020-11-26
EP3810639A2 (en) 2021-04-28

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