CN110384179A - Method, feed addictive and the application of fermentation distillers ' grains are produced using bacillus subtilis - Google Patents
Method, feed addictive and the application of fermentation distillers ' grains are produced using bacillus subtilis Download PDFInfo
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- CN110384179A CN110384179A CN201910786555.6A CN201910786555A CN110384179A CN 110384179 A CN110384179 A CN 110384179A CN 201910786555 A CN201910786555 A CN 201910786555A CN 110384179 A CN110384179 A CN 110384179A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
- A23K10/38—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material from distillers' or brewers' waste
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/25—Shaping or working-up of animal feeding-stuffs by extrusion
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a kind of method, feed addictive and applications that fermentation distillers ' grains are produced using bacillus subtilis, it first pre-processes distillers ' grains, to remove rice husk and alcoholic content therein, then fermentation medium base-material is formed after mixing with expanded corn powder, after adding metal ion, pH value is adjusted with calcium hydroxide again, it is fermented using bacillus subtilis to culture medium base-material, to obtain feed addictive, the feed addictive and animal diets cooperate feeding animals, the speed of growth of animal can be improved, improve immunity, reduce diarrhea rate.
Description
Technical field
The invention belongs to animal feed preparation technical fields, and in particular to a kind of white using bacillus subtilis production fermentation
Method, feed addictive and the application of vinasse.
Background technique
Fresh distillers ' grains are using grain as primary raw material, using unique solid state fermentation and solid state distillation conventional brew technique system
By-product after obtaining white wine.Since Starch Conversion in grain is mainly alcohol by the biofermentation during wine brewing, although because
The content of raw materials used, technique difference, nutritional ingredient and same ingredient in vinasse is not also identical, but ingredient is substantially close.
Nutritional ingredient in vinasse is in addition to being not thorough remaining portion raw material residue from saccharification, fermentation, mainly from thallus and its newly
Old metabolite and thallus autolysate, while saccharomycete, active peptide, organic acid and some bioactivity are also generated in fermentation process
The factor, such as purine, pyrimidine, lipids, enzyme, this is that general cereal cannot compare.However also contain in vinasse
The fermentation filler of 40%-50% --- rice husk (wherein containing cellulose, lignin and SiO2), even if fermentation process is in various enzymes
Under the action of, fiber is degraded, but crude fibre is still up to 20% or so, seriously affects the digestion and absorption of livestock and poultry, is reduced
The nutritive values of vinasse.In addition, fresh distillers ' grains moisture is up to 60% or so, if be pocessed not in time, especially summer and autumn
High temperature season, will be putrid and deteriorated, not only wastes valuable resource, can also seriously pollute ambient enviroment.In addition, in distillers ' grains
Remaining certain alcohol, has certain adverse effect to the growth of the animal of certain specific periods, for example, due to containing in vinasse
The rice husk of 40%-50%, according to analysis, crude fiber content 44.5%, content of lignin 21.4%, content of ashes in rice husk
19.9%, and 90% or more be SiO in ash content2, livestock and poultry cannot not only provide nutrition after eating, also to pay a large amount of energy instead
Amount digestion rice husk.Feeding practice confirms, feeds wet nurse pig with the fresh grain containing rice husk, the decline of sow milk quality causes piglet to draw dysentery；
Feeding boar keeps boar sperm lopsided, reduces sow conception rate, and causes boar to be short of breath, the diseases such as palpitating speed, vessel retraction
Shape affects the normal development of pig；In addition, distillers ' grains are hot big, it is not suitable for summer feeding.As it can be seen that although fresh distillers ' grains are sought
Material abundance is supported, but and is not suitable for Direct-fed animal.
Summary of the invention
One of the objects of the present invention is to provide a kind of methods for producing fermentation distillers ' grains using bacillus subtilis, lead to
It crosses after handling fresh distillers ' grains and carries out fermentation acquisition fermentation distillers ' grains with bacillus subtilis, can drop to greatest extent
The adverse effect of low fresh distillers ' grains；
The second object of the present invention is to provide a kind of feed addictive, passes through the distillers ' grains that will ferment and carry out drying and processing
After obtain；
The third object of the present invention is to provide a kind of application of feed addictive.
To reach above-mentioned technical purpose, The technical solution adopted by the invention is as follows:
Technical solution one:
A method of fermentation distillers ' grains are produced using bacillus subtilis, comprising the following steps:
(1) distillers ' grains pre-process: fresh distillers ' grains being gone after rice husk to carry out sterilization treatment, dries, crushes after cooling；It goes out
Bacterium process uses pasteurization, principle are as follows: fresh distillers ' grains is heated to 68~70 DEG C, and after keeping this temperature 30min
Rapid cooling is to 4-5 DEG C.Because the killing point of general bacterium is in 68 DEG C of temperature, time 30min hereinafter, by fresh distillers ' grains
After the processing of this method, pathogenic bacteria therein and most non-pathogenic bacterias can be killed；After mixed raw material heating suddenly
Cooling, hot and cold variation sharply can also promote the death of bacterium；The process of drying is also the process except alcohol, due to alcohol
Low boiling point cooperates stirring in drying course, can remove the alcohol of wherein most；For the ease of fermentation, distillers ' grains are crushed
It is advisable to 80-100 mesh；
(2) expanding treatment: corn flour after crushing and/or corn protein powder are subjected to low temperature expanding, then carry out two wheat-middlings
It is broken, it sieves with 100 mesh sieve, forms expanded corn powder and/or popcorn albumen powder is spare；
Wherein expanded corn powder is to carry out extrusion after being ground into bulky grain powder with maize pulp, then through flour mill two
It is prepared after secondary crushing；
In corn flour about containing protein about 8.5%, fat about 4.3%, carbohydrate about 73.2%, calcium about 0.022%, phosphorus
0.21%, iron about 0.0016%, in addition, also containing carrotene, vitamin B1, B2 and niacin and sitosterol, lecithin,
Vitamin E, lysine etc..Extrusion processing can promote 1,4- glycosidic bond in starch molecule to be broken and generate glucose, malt
The low molecular weight products such as sugar, maltotriose and maltodextrin, can promote the absorption of microorganism；Extrusion is to the main of starch
Effect is that its intermolecular hydrogen bonding is promoted to be broken and be gelatinized.Starch after gelatinization can a large amount of water swellings, increase starch and connect with enzyme
The chance of touching, to further increase starch to the bioavailability of microorganism；In addition, utilizing withered grass bud by raw material of corn flour
Spore bacillus carries out fermentation and prepares lactic acid, and process is bacillus subtilis using corn flour as nutriment, and corn flour is absorbed it
Decomposition and inversion is carried out to microorganism using intracorporal predetermined substance afterwards, finally obtains lactic acid.Starch after extrusion has fluffy
The porous structure of pine, the specific decomposed substance enters in the porous structure of corn flour after corn flour is absorbed by the micro-organisms, by
In the increase of corn flour specific surface area, the contact area with decomposed substance is improved, is extended in the stop of inside microbes
Between, to improve conversion ratio；In addition, corn flour is many by containing in the loose porous structure of formation after extrusion
Active catalyst sites, on the one hand these active sites can be improved the activity of microorganism, on the other hand can also improve decomposition
The catalytic activity of substance, to further improve the conversion ratio of corn flour.
Extruding and puffing technology is the technology for integrating mixing, stirring, crushing, heating, sterilization, extruding and molding etc., main
To be applied to the processing of cereal.Extrusion is to feed material in extruder, by screw rod transmission, by friction, high pressure and
High temperature makes the material serialization of a series of complex such as be squeezed, melted, sterilized cross processing, and finally, material is from equipment end quilt
It squeezes out, pressure is down to normality suddenly.It squeezes and generates high temperature and pressure, grain component is made to accumulate big energy, released suddenly under normal conditions,
Increase direction to entropy to carry out, extruding occurs.For the present invention with the corn flour of extrusion for main fermenting raw materials lactic acid producing, base-material is beautiful
Rice flour will form cellular after expanding treatment, conducive to the progress of fermentation.In addition base-material cost price is cheap, not will cause
The waste of resource, meets the strategy of sustainable development.
Corn protein powder is also corn gluten meal, is that through wet-grinding technology and relative device corn coarse starch milk is made in niblet, by hydrolysis,
Separation, concentration, fermentating drying are made, protein content 20%-70%, it is mainly made of zein, contains a small amount of shallow lake
Powder and fiber.
(3) it prepares seed liquor: bacillus subtilis fluid nutrient medium activation being followed by being commissioned to train feeding, be prepared into seed liquor；
Wherein bacillus subtilis is preferably commercial product；
(4) it ferments: by the expanded corn powder prepared in pretreated distillers ' grains in step (1) and step (2) and/or swollen
Change corn protein powder so that fermentation medium base-material is made after the mass ratio mixing of 6-8:4-2, metal ion inorganic salts is added, then
Adjust pH to 5.5-6.5；Seed liquor is added, the additive amount of seed liquor is 10wt%, forms fermentation raw material, fermentation raw material is tiled
Solid state fermentation is carried out in fermentation vat；Fermentation condition: humidity 70-75%, 36-38 DEG C of temperature；It ferments to after 18, it is logical to increase steam
Amount improves air humidity to 80-85%, and ferment 48-50h, terminates fermentation, obtains tunning.
As a further improvement of the present invention, the distillers ' grains go rice husk process as follows: after fresh distillers ' grains are dried
Carry out it is mechanically decoupled, pass through screening removal rice husk.Due to thering is substantial portion of rice husk to be all wrapped in fresh distillers ' grains
Grain, it is difficult to separate, first it dried, then by separation machinery carry out grind stranding method shelling, finally by point
Its rice husk is separated in grade screening, obtains the distillers ' grains without rice husk.Wherein separation machinery is conventional shelling machine, machine
Tool structure and working principle are routine techniques, and this will not be repeated here.
As a further improvement of the present invention, in step (2) expansion rate of corn flour extrusion between 5-8%.
As a further improvement of the present invention, the additive amount of the metal ion inorganic salts is 80g/t；The metal ion
Inorganic salts are the salt-mixture that manganese sulfate, magnesium sulfate and dipotassium hydrogen phosphate are formed with the mass ratio of 1:4:5.
As a further improvement of the present invention, fermentation raw material described in step (4) tile in fermentation vat with a thickness of 4-
10cm。
As a further improvement of the present invention, pH is adjusted to be adjusted using calcium hydroxide in the process.
Technical solution two:
A kind of feed addictive dries the fermentation liquid obtained using the method for bacillus subtilis production fermentation distillers ' grains
After be prepared into feed addictive.
Technical solution three:
A kind of application of feed addictive in animal feed, makes an addition in the non-breeding season feed diet of pig, ox and sheep,
Instead of the daily ration of 25-35%.
Compared with prior art, the present invention has the following technical effect that
Distillers ' grains are the Main By products of generation during wine brewing, and price is low, and supply is very sufficient, the master of vinasse
It wants ingredient to have protein, cellulose, fat, vitamin and various microelements, is sold as normal diet.But
It is to use the livestocks such as fresh grain stillage Direct-fed ox, sheep, containing the crude fibre for being largely not easy to be digested and assimilated by animal in vinasse, therefore feeds
Less effective is supported, economic value added is lower.And fresh grain stillage easily goes mouldy, alcohol content is high, contains anti-nutrient substance simultaneously, and it is excessive
Direct-fed easily causes livestock poisoning or influences livestock growth and development.One aspect of the present invention is by going rice husk process by fresh white wine
Rice husk and alcohol removal in grain, reduce adverse effect of the distillers ' grains to feeding animals to greatest extent.
Bacillus subtilis (Bacillus subtilis) is one kind of bacillus, is generated during thalli growth
Subtilin, polymyxins, nystatin, gramicidins isoreactivity substance, the conditions of pathogenic bacteria or autogenous infection is caused
Germ has obvious inhibiting effect；Or free oxygen is consumed rapidly, low-oxygen environment is caused, promotes to grow beneficial to anaerobic bacteria, press down indirectly
Make other pathogenic bacteria growths.Bacillus subtilis (Bacillus subtilis) is gram-positive bacteria, is that a kind of aerobic is thin
Bacterium has the characteristics such as high temperature resistant, bile tolerance, acid resistance, endogenous spermatium can be generated in extreme circumstances, after feed granulating
Also it is able to maintain high survival rate.Bacillus subtilis consumes oxygen in body, discharges propionic acid, acetic acid, and butyric acid etc. can be created
One slightly sour environment of anaerobism promotes to grow beneficial to anaerobic bacteria, inhibits harmful bacteria growth, adjusts intestinal microecology balance, and enhancing is anti-
Poison, disease resistance can also generate a variety of digestive ferments such as amylase, protease etc. and promote forage degradation, improve efficiency of feed utilization.
The present invention utilizes fermentation of bacillus subtilis distillers ' grains.Bacillus subtilis consumes oxygen in body, discharges propionic acid, acetic acid,
Butyric acid etc. can create the slightly sour environment of anaerobism, promote to grow beneficial to anaerobic bacteria, inhibit harmful bacteria growth, adjust the micro- life of enteron aisle
State balance, enhances antitoxin, disease resistance, can also generate a variety of digestive ferments such as amylase, protease etc. and promote forage degradation, mention
High efficiency of feed utilization.
Specific embodiment
It should be understood that it is to describe special embodiment that heretofore described term, which is only, it is not intended to limit this hair
It is bright.In addition, for the numberical range in the present invention, it is thus understood that it is also specifically disclosed that between the upper limit value and lower limit value of the range
Each median.Median and any other statement value in any statement value or stated ranges or in the range
Lesser range is also included in the present invention each of between interior median.These small range of upper limit values and lower limit value can
Independently include or exclude in range.
In addition, in order to better illustrate the present invention, being given in specific embodiment below numerous specific
Details.It will be appreciated by those skilled in the art that without certain details, the present invention equally be can be implemented.In other reality
It applies in example, method well known to those skilled in the art, means is not described in detail, in order to highlight purport of the invention.
In the present invention, indicate that the unit of material content is based on parts by weight meter, unless otherwise stated.In addition, about technology of the invention
The measuring method of index is in the art using standard method, and for details, reference can be made to newest national standards, unless otherwise stated.
Bacillus subtilis used in following embodiment is commercial product, purchased from the limited public affairs of the vast and boundless biotechnology of Weifang benefit
Department.
Embodiment 1:
The feed addictive of the present embodiment, preparation process are as follows:
(1) will fresh distillers ' grains dry after carry out mechanically decoupled, rice husk is removed by gradation sizing, then crushed
100 meshes；
(2) it is ground into corn quarrel after niblet being peeled, corn quarrel is subjected to extrusion, extruding in extruder
Screw rod of puffing machine revolving speed is 150rpm/min in the process, and processing temperature is 140 DEG C, then expansion rate 15% carries out two wheat-middlings
It is broken, it sieves with 100 mesh sieve, obtains expanded corn powder；
(3) bacillus subtilis fluid nutrient medium activation is followed by being commissioned to train feeding, connects feeding 2-3 generation of being commissioned to train, is prepared into withered grass
Bacillus seed liquor；
(4) processed distillers ' grains are mixed with expanded corn powder according to the mass ratio of 8:2；By manganese sulfate, magnesium sulfate and
Dipotassium hydrogen phosphate is to form metal ion inorganic salts, additive amount 80g/t after the mass ratio mixing of 1:4:5；Then hydroxide is used
Calcium adjusts pH to 6.0；It is configured to fermentation medium base-material；
(5) seed liquor is added into fermentation medium base-material, the additive amount of seed liquor is 10wt%, fermentation raw material is formed,
Fermentation raw material is laid in fermentation vat and carries out solid state fermentation；Fermentation condition: humidity 70%, 38 DEG C of temperature；It ferments to after 18, increases
Big vapor flux improves air humidity to 80%, and ferment 50h, terminates fermentation, obtains tunning；
(6) cooling up to feed addictive after drying fermentation liquid.
Embodiment 2:
The feed addictive of the present embodiment, preparation process are as follows:
(1) will fresh distillers ' grains dry after carry out mechanically decoupled, rice husk is removed by gradation sizing, then crushed
100 meshes；
(2) corn protein powder is carried out to extrusion in extruder, screw rod of puffing machine revolving speed is in puffing process
150rpm/min, processing temperature are 140 DEG C, and then expansion rate 15% carries out separating twice, sieves with 100 mesh sieve, obtain extruding egg
Cream meal；
(3) bacillus subtilis fluid nutrient medium activation is followed by being commissioned to train feeding, connects feeding 2-3 generation of being commissioned to train, is prepared into withered grass
Bacillus seed liquor；
(4) processed distillers ' grains are mixed with expanded corn powder according to the mass ratio of 8:2；By manganese sulfate, magnesium sulfate and
Dipotassium hydrogen phosphate is to form metal ion inorganic salts, additive amount 80g/t after the mass ratio mixing of 1:4:5；Then hydroxide is used
Calcium adjusts pH to 6.0；It is configured to fermentation medium base-material；
(5) seed liquor is added into fermentation medium base-material, the additive amount of seed liquor is 10wt%, fermentation raw material is formed,
Fermentation raw material is laid in fermentation vat and carries out solid state fermentation；Fermentation condition: humidity 70%, 38 DEG C of temperature；It ferments to after 18, increases
Big vapor flux improves air humidity to 80%, and ferment 50h, terminates fermentation, obtains tunning；
(6) cooling up to feed addictive after drying fermentation liquid.
Embodiment 3:
The feed addictive of the present embodiment, preparation process are as follows:
(1) will fresh distillers ' grains dry after carry out mechanically decoupled, rice husk is removed by gradation sizing, then crushed
100 meshes；
(2) it is ground into corn quarrel after niblet being peeled, corn quarrel is mixed with treated distillers ' grains by the mass ratio of 2:8
Extrusion is carried out in extruder simultaneously after closing uniformly, screw rod of puffing machine revolving speed is 150rpm/min in puffing process,
Processing temperature is 140 DEG C, then expansion rate 15% carries out separating twice, sieves with 100 mesh sieve；
(3) bacillus subtilis fluid nutrient medium activation is followed by being commissioned to train feeding, connects feeding 2-3 generation of being commissioned to train, is prepared into withered grass
Bacillus seed liquor；
(4) it is inorganic that metal ion is formed after mixing manganese sulfate, magnesium sulfate and dipotassium hydrogen phosphate with the mass ratio of 1:4:5
Salt, the mixing species after extruding is added, additive amount 80g/t；Then pH to 6.0 is adjusted with calcium hydroxide；It is configured to fermentation training
Support base base-material；
(5) seed liquor is added into fermentation medium base-material, the additive amount of seed liquor is 10wt%, fermentation raw material is formed,
Fermentation raw material is laid in fermentation vat and carries out solid state fermentation；Fermentation condition: humidity 70%, 38 DEG C of temperature；It ferments to after 18, increases
Big vapor flux improves air humidity to 80%, and ferment 50h, terminates fermentation, obtains tunning；
(6) cooling up to feed addictive after drying fermentation liquid.
Embodiment 4:
The feed addictive of the present embodiment, preparation process are as follows:
(1) will fresh distillers ' grains dry after carry out mechanically decoupled, rice husk is removed by gradation sizing, then crushed
100 meshes；
(2) corn protein powder is swollen in extruding simultaneously after mixing with the mass ratio of 2:8 with processed distillers ' grains
Extrusion is carried out in change machine, screw rod of puffing machine revolving speed is 150rpm/min in puffing process, and processing temperature is 140 DEG C, extruding
Rate is 15%, then carries out separating twice, sieves with 100 mesh sieve；
(3) bacillus subtilis fluid nutrient medium activation is followed by being commissioned to train feeding, connects feeding 2-3 generation of being commissioned to train, is prepared into withered grass
Bacillus seed liquor；
(4) metal ion inorganic salts are formed after mixing manganese sulfate, magnesium sulfate and dipotassium hydrogen phosphate with the mass ratio of 1:4:5
In mixed material after being added to extruding, additive amount 80g/t；Then pH to 6.0 is adjusted with calcium hydroxide；It is configured to fermentation training
Support base base-material；
(5) seed liquor is added into fermentation medium base-material, the additive amount of seed liquor is 10wt%, fermentation raw material is formed,
Fermentation raw material is laid in fermentation vat and carries out solid state fermentation；Fermentation condition: humidity 70%, 38 DEG C of temperature；It ferments to after 18, increases
Big vapor flux improves air humidity to 80%, and ferment 50h, terminates fermentation, obtains tunning；
(6) cooling up to feed addictive after drying fermentation liquid.
Embodiment 5:
The feed addictive of the present embodiment and the difference of embodiment 4 are only that: the mass ratio of distillers ' grains and corn protein powder
For 7:3, remaining preparation method is the same as embodiment 4.
Embodiment 6:
The feed addictive of the present embodiment and the difference of embodiment 4 are only that: the mass ratio of distillers ' grains and corn protein powder
For 6:4, remaining preparation method is the same as embodiment 4.
Embodiment 7:
The feed addictive of the present embodiment and the difference of embodiment 4 are only that: the expansion rate of puffing process is 10%, remaining
Preparation method is the same as embodiment 4.
Embodiment 8:
The feed addictive of the present embodiment and the difference of embodiment 4 are only that: the expansion rate of puffing process is 8%, remaining
Preparation method is the same as embodiment 4.
Comparative example 1:
The feed addictive of this comparative example, preparation process are as follows:
(1) will fresh distillers ' grains dry after carry out mechanically decoupled, rice husk is removed by gradation sizing, then crushed
100 meshes；
(2) bacillus subtilis fluid nutrient medium activation is followed by being commissioned to train feeding, connects feeding 2-3 generation of being commissioned to train, is prepared into withered grass
Bacillus seed liquor；
(3) corn protein powder is uniformly mixed with processed distillers ' grains with the mass ratio of 2:8, by manganese sulfate, sulfuric acid
Magnesium and dipotassium hydrogen phosphate are formed metal ion inorganic salts after being mixed with the mass ratio of 1:4:5 and are added to distillers ' grains and corn protein powder
Mixed material in, additive amount 80g/t；Then pH to 6.0 is adjusted with calcium hydroxide；It is configured to fermentation medium base-material；
(4) seed liquor is added into fermentation medium base-material, the additive amount of seed liquor is 10wt%, fermentation raw material is formed,
Fermentation raw material is laid in fermentation vat and carries out solid state fermentation；Fermentation condition: humidity 70%, 38 DEG C of temperature；It ferments to after 18, increases
Big vapor flux improves air humidity to 80%, and ferment 50h, terminates fermentation, obtains tunning；
(5) cooling up to feed addictive after drying fermentation liquid.
Comparative example 2:
The feed addictive of this comparative example, preparation process are as follows:
(1) it by fresh distillers ' grains dries pulverizing, then crushes and sieves with 100 mesh sieve；
(2) by corn protein powder and distillers ' grains with the mass ratio of 2:8 after mixing simultaneously in extruder into
Row extrusion, screw rod of puffing machine revolving speed is 150rpm/min in puffing process, and processing temperature is 140 DEG C, expansion rate 15%,
Then separating twice is carried out, is sieved with 100 mesh sieve；
(3) bacillus subtilis fluid nutrient medium activation is followed by being commissioned to train feeding, connects feeding 2-3 generation of being commissioned to train, is prepared into withered grass
Bacillus seed liquor；
(4) metal ion inorganic salts are formed after mixing manganese sulfate, magnesium sulfate and dipotassium hydrogen phosphate with the mass ratio of 1:4:5
In mixed material after being added to extruding, additive amount 80g/t；Then pH to 6.0 is adjusted with calcium hydroxide；It is configured to fermentation training
Support base base-material；
(5) seed liquor is added into fermentation medium base-material, the additive amount of seed liquor is 10wt%, fermentation raw material is formed,
Fermentation raw material is laid in fermentation vat and carries out solid state fermentation；Fermentation condition: humidity 70%, 38 DEG C of temperature；It ferments to after 18, increases
Big vapor flux improves air humidity to 80%, and ferment 50h, terminates fermentation, obtains tunning；
(6) cooling up to feed addictive after drying fermentation liquid.
Comparative example 3:
The feed addictive preparation method of this comparative example is as follows:
(1) will fresh distillers ' grains dry after carry out mechanically decoupled, rice husk is removed by gradation sizing, then crushed
100 meshes；
(2) it is ground into corn quarrel after niblet being peeled, corn quarrel is mixed with treated distillers ' grains by the mass ratio of 2:8
Extrusion is carried out in extruder simultaneously after closing uniformly, screw rod of puffing machine revolving speed is 150rpm/min in puffing process,
Processing temperature is 140 DEG C, then expansion rate 15% carries out separating twice, sieves with 100 mesh sieve, and forms fermentation medium base-material；
(3) bacillus subtilis fluid nutrient medium activation is followed by being commissioned to train feeding, connects feeding 2-3 generation of being commissioned to train, is prepared into withered grass
Bacillus seed liquor；
(4) seed liquor is added into fermentation medium base-material, the additive amount of seed liquor is 10wt%, fermentation raw material is formed,
Fermentation raw material is laid in fermentation vat and carries out solid state fermentation；Fermentation condition: humidity 70%, 38 DEG C of temperature；It ferments to after 18, increases
Big vapor flux improves air humidity to 80%, and ferment 50h, terminates fermentation, obtains tunning；
(5) cooling up to feed addictive after drying fermentation liquid.
The feed addictive of the feed addictive of embodiment 1-8 and comparative example 1-3 are subjected to nutritional ingredient detection, detection knot
Fruit is shown in Table 1.
1 nutritional ingredient of table changes table
Note: ﹢ represents the raising of content, with total protein content in distillers ' grains and corn flour/corn protein powder before fermenting, solvable
Property protein content and cellulose protein content on the basis of.
The large-scale pig farm of Shandong Rizhao is chosen, the pig at -4 monthly age of 3 monthly age is randomly divided into 12 groups, every group 5, will be implemented
The feed addictive of example 1-8 and the feed addictive of comparative example 1-3 replace 20% daily ration, for feeding pig.Blank control group
Only edible daily ration.
After feeding 30 days, the upgrowth situation of each group experiment pig is counted, statistical result is shown in Table 2.
Table 2
Xingtai large-scale beef cattle breeding field is chosen, the ox at 7-8 monthly age is randomly divided into 12 groups, it every group 10, will be real
The feed addictive of the feed addictive and comparative example 1-3 of applying a 1-8 replaces 20% daily ration, for feeding ox.Blank control
Organize only edible daily ration.
After feeding 90 days, the upgrowth situation of each group experiment ox is counted, statistical result is shown in Table 3.
Table 3
| Gain in weight % | Diarrhea total degree | The dead quantity | |
| Embodiment 1 | 35 | 2 | 0 |
| Embodiment 2 | 37 | 2 | 0 |
| Embodiment 3 | 39 | 1 | 0 |
| Embodiment 4 | 42 | 3 | 0 |
| Embodiment 5 | 36 | 2 | 0 |
| Embodiment 6 | 35 | 1 | 0 |
| Embodiment 7 | 37 | 4 | 0 |
| Embodiment 8 | 39 | 3 | 0 |
| Comparative example 1 | 30 | 9 | 0 |
| Comparative example 2 | 25 | 17 | 1 |
| Comparative example 3 | 29 | 12 | 0 |
By small range experimental verification, it is equally applicable, passes through when the feed addictive of embodiment 1-8 is applied to feeding sheep
Cross comparison, the sheep of the feed addictive of addition 25% is not than adding the sheep of feed addictive gain in weight ratio after 40 days in daily ration
The high 15-21% of blank control group.
The above embodiments are only used to illustrate the present invention, rather than limitation of the present invention.Although referring to embodiment to this hair
It is bright to be described in detail, those skilled in the art should understand that, to technical solution of the present invention carry out it is various combination,
Modification or equivalent replacement should all cover and want in right of the invention without departure from the spirit and scope of technical solution of the present invention
It asks in range.
Claims (8)
1. a kind of method for producing fermentation distillers ' grains using bacillus subtilis, which comprises the following steps:
(1) distillers ' grains pre-process: fresh distillers ' grains being gone after rice husk to carry out sterilization treatment, dries, crushes after cooling；
(2) expanding treatment: corn flour after crushing and/or corn protein powder are subjected to low temperature expanding, then carry out separating twice, mistake
100 meshes, form expanded corn powder and/or popcorn albumen powder is spare；
(3) it prepares seed liquor: bacillus subtilis fluid nutrient medium activation being followed by being commissioned to train feeding, be prepared into seed liquor；
(4) it ferments: the expanded corn powder and/or extruding that prepare in pretreated distillers ' grains in step (1) and step (2) is beautiful
Rice gluten powder is added metal ion inorganic salts, then adjusts so that fermentation medium base-material is made after the mass ratio mixing of 6-8:4-2
PH to 5.5-6.5；Seed liquor is added, the additive amount of seed liquor is 10wt%, forms fermentation raw material, fermentation raw material is laid in hair
Solid state fermentation is carried out in ferment pond；Fermentation condition: humidity 70-75%, 36-38 DEG C of temperature；It ferments to after 18, increases vapor flux,
Air humidity is improved to 80-85%, ferment 48-50h, terminates fermentation, obtains tunning.
2. the method according to claim 1 for producing fermentation distillers ' grains using bacillus subtilis, which is characterized in that described
Distillers ' grains go rice husk process as follows: will fresh distillers ' grains dry after carry out mechanically decoupled, pass through screening removal rice husk.
3. the method according to claim 1 for producing fermentation distillers ' grains using bacillus subtilis, which is characterized in that step
(2) expansion rate of corn flour extrusion is between 5-8% in.
4. the method according to claim 1 for producing fermentation distillers ' grains using bacillus subtilis, which is characterized in that described
The additive amount of metal ion inorganic salts is 80g/t；The metal ion inorganic salts be manganese sulfate, magnesium sulfate and dipotassium hydrogen phosphate with
The salt-mixture that the mass ratio of 1:4:5 is formed.
5. the method according to claim 1 for producing fermentation distillers ' grains using bacillus subtilis, which is characterized in that step
(4) fermentation raw material described in tile in fermentation vat with a thickness of 4-10cm.
6. the method according to claim 5 for producing fermentation distillers ' grains using bacillus subtilis, which is characterized in that adjust
It is adjusted during pH using calcium hydroxide.
7. a kind of feed addictive, which is characterized in that produced described in any one of claims 1-6 using bacillus subtilis
Feed addictive is prepared into after the fermentation liquid drying that the method for fermentation distillers ' grains obtains.
8. application of the feed addictive as claimed in claim 7 in animal feed, which is characterized in that make an addition to pig, Niu He
In the non-breeding season feed diet of sheep, instead of the daily ration of 25-35%.
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