CN110381961A - 用于治疗用途的细胞和组合物的制备中的确定性侧向位移 - Google Patents
用于治疗用途的细胞和组合物的制备中的确定性侧向位移 Download PDFInfo
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0636—T lymphocytes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0652—Sorting or classification of particles or molecules
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/33—Fusion polypeptide fusions for targeting to specific cell types, e.g. tissue specific targeting, targeting of a bacterial subspecies
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Abstract
Description
Claims (155)
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PCT/US2017/057876 WO2018080997A1 (en) | 2016-10-24 | 2017-10-23 | Deterministic lateral displacement in the preparation of cells and compositions for therapeutic uses |
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CN115209996A (zh) * | 2019-12-28 | 2022-10-18 | Gpb科学有限公司 | 用于处理颗粒和细胞的微流体盒 |
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EP3569313A1 (en) | 2013-03-15 | 2019-11-20 | GPB Scientific, LLC | On-chip microfluidic processing of particles |
US20150064153A1 (en) | 2013-03-15 | 2015-03-05 | The Trustees Of Princeton University | High efficiency microfluidic purification of stem cells to improve transplants |
WO2014145075A2 (en) | 2013-03-15 | 2014-09-18 | The Trustees Of Princeton University | Methods and devices for high throughpout purification |
US10976232B2 (en) | 2015-08-24 | 2021-04-13 | Gpb Scientific, Inc. | Methods and devices for multi-step cell purification and concentration |
WO2019046052A1 (en) | 2017-09-01 | 2019-03-07 | Gpb Scientific, Llc | METHODS FOR PREPARING THERAPEUTICALLY ACTIVE CELLS USING MICROFLUIDIC |
CN107723207B (zh) * | 2017-11-01 | 2019-01-01 | 深圳市瑞格生物科技有限公司 | 一种分离捕获细胞的芯片及其在肿瘤细胞分选中的应用 |
JP2021522834A (ja) * | 2018-05-13 | 2021-09-02 | ジーピービー・サイエンティフィック・インコーポレイテッドGPB Scientific, Inc. | 生成物の再循環を用いた決定論的横方向変位(Deterministic Lateral Displacement)による結合精製および濃縮 |
CN108841727B (zh) * | 2018-05-31 | 2022-03-04 | 东南大学 | 基于微流控芯片的car-t细胞自动化制备系统 |
CN110643577A (zh) * | 2018-06-26 | 2020-01-03 | 深圳市北科生物科技有限公司 | 基于机械臂的全自动细胞培养方法及其系统 |
WO2020014538A1 (en) * | 2018-07-12 | 2020-01-16 | Gpb Scientific, Llc | Microfluidic methods for the preparation of cells |
AU2020216127A1 (en) * | 2019-01-29 | 2021-09-23 | Gpb Scientific, Inc. | Cell populations with improved production and therapeutic characteristics |
EP4093853A4 (en) * | 2020-01-20 | 2024-05-29 | Gpb Scient Inc | PREVENTION OF PLATELET ACTIVATION AND CELL DIFFERENTIATION DURING PROCESSING OF BLOOD AND BLOOD-ASSOCIATED COMPOSITIONS |
EP4237578A1 (en) * | 2020-10-30 | 2023-09-06 | Onecyte Biotechnologies, Inc. | Systems and methods for high-throughput cell line development |
CN116783283A (zh) * | 2020-11-13 | 2023-09-19 | Gpb科学有限公司 | 产生具有增加的核酸摄取的细胞群体的方法 |
WO2022104124A1 (en) * | 2020-11-13 | 2022-05-19 | Gbp Scientific, Inc. | Methods for producing cell populations with increased nucleic acid uptake |
CN113462522B (zh) * | 2021-08-09 | 2023-04-25 | 江西中医药大学 | 一种从体外血液分离磁珠的确定性侧向位移微流控芯片 |
US20230139871A1 (en) * | 2021-11-02 | 2023-05-04 | Terumo Bct, Inc. | Systems and Methods For Using Microfluidic Devices With Apheresis Systems |
WO2023114919A1 (en) * | 2021-12-15 | 2023-06-22 | Gpb Scientific, Inc. | Label-free cell isolation |
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JP4259825B2 (ja) * | 2002-08-20 | 2009-04-30 | 株式会社日立メディコ | 細胞分離キットおよびそれを用いた細胞分離方法 |
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WO2012091056A1 (ja) * | 2010-12-28 | 2012-07-05 | エスシーワールド株式会社 | 血液中の標的細胞の検査方法、標的細胞検索装置、及びバイオチップ |
WO2014145075A2 (en) * | 2013-03-15 | 2014-09-18 | The Trustees Of Princeton University | Methods and devices for high throughpout purification |
EP3569313A1 (en) * | 2013-03-15 | 2019-11-20 | GPB Scientific, LLC | On-chip microfluidic processing of particles |
SG2013090790A (en) * | 2013-12-04 | 2015-07-30 | Clearbridge Mfluidics Pte Ltd | A microfluidic device |
JP6661544B2 (ja) * | 2014-04-24 | 2020-03-11 | ミルテニイ バイオテック ゲゼルシャフト ミット ベシュレンクテル ハフツング | 遺伝子改変したt細胞の自動生成法 |
MX370018B (es) * | 2014-04-25 | 2019-11-28 | Bluebird Bio Inc | Metodos mejorados para la elaboracion de terapias celulares adoptivas. |
CN107250343B (zh) * | 2015-02-27 | 2020-12-29 | 凸版印刷株式会社 | 分离细胞的方法及用于该方法的器件 |
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US20020058019A1 (en) * | 2000-02-24 | 2002-05-16 | Ronald Berenson | Simultaneous stimulation and concentration of cells |
US20040224402A1 (en) * | 2003-05-08 | 2004-11-11 | Xcyte Therapies, Inc. | Generation and isolation of antigen-specific T cells |
US20070099207A1 (en) * | 2005-04-05 | 2007-05-03 | Martin Fuchs | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
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CN115209996A (zh) * | 2019-12-28 | 2022-10-18 | Gpb科学有限公司 | 用于处理颗粒和细胞的微流体盒 |
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WO2018080997A1 (en) | 2018-05-03 |
AU2017350739A1 (en) | 2019-06-13 |
JP2020503887A (ja) | 2020-02-06 |
CA3041522A1 (en) | 2018-05-03 |
AU2017350739B2 (en) | 2023-03-16 |
US20190366342A1 (en) | 2019-12-05 |
EP3528823A1 (en) | 2019-08-28 |
EP3528823A4 (en) | 2020-04-15 |
JP2023022241A (ja) | 2023-02-14 |
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