CN110354184B

CN110354184B - External medicine for treating female cervical cancer and preparation method thereof

Info

Publication number: CN110354184B
Application number: CN201910344175.7A
Authority: CN
Inventors: 王天强; 周云贵
Original assignee: Chenzhou Tianzhiyu Health Management Co ltd
Current assignee: Chenzhou Tianzhiyu Health Management Co ltd
Priority date: 2019-04-26
Filing date: 2019-04-26
Publication date: 2021-06-08
Anticipated expiration: 2039-04-26
Also published as: CN110354184A

Abstract

The invention discloses an external medicine for treating female cervical cancer, which is prepared from raw materials including an L-40 amphiphilic transdermal agent, a traditional Chinese medicine composition and protoplasm mature vinegar in a mass ratio of 1:5-6, wherein the traditional Chinese medicine composition comprises: centipede, typhonium giganteum, angelica, brucea javanica, dysosma versipellis rhizome, root and stem of Tamarigold, Anemone davidiana, golden larch bark, nux vomica, cyrtomium rhizome, myrrh, Japanese creeper, polyrhachis vicina, rangooncreeper fruit, pepper, leech, astragalus mongholicus and radix scrophulariae. A method for preparing topical medicine for treating female cervical cancer is provided. The external medicine has the synergistic effect of the components, has the effects of clearing away heat and toxic materials, strengthening body resistance to eliminate pathogenic factors, promoting blood circulation to remove blood stasis and softening hardness to eliminate tumor, and has good prevention and treatment effects on cervical cancer.

Description

External medicine for treating female cervical cancer and preparation method thereof

Technical Field

The invention relates to the field of traditional Chinese medicine. More particularly, the invention relates to an external medicine for treating female cervical cancer and a preparation method thereof.

Background

Cervical cancer is the most common gynecological malignancy, usually occurring at the junction of squamous epithelial cells of the cervical-vaginal part or transitional zone and columnar epithelial cells of the endocervical lining, 99.8% of cervical cancers are caused by Human Papilloma Virus (HPV) infection, and 95% of women have at least 1 chance of infecting papilloma virus (HPV) in their lifetime, which is not the first cause of the disease but is not the human body to sit to kill, and the women have the ability to expel toxin by themselves, so that the papilloma virus (HPV) is not wanted in vivo, and can be excreted in 8-12 months after infecting the papilloma virus (HPV), thereby keeping the healthy state. The real reason is that the resistance of women is low, so that Human Papillomavirus (HPV) is kept at cervical squamous junction for a long time, generally for more than 12 months, and then Cervical Intraepithelial Neoplasia (CIN) is caused, so that the continuous papillomavirus (HPV) infection is the real reason of cervical cancer, and the earliest cervical intraepithelial neoplasia belongs to low-grade lesions.

The traditional cervical cancer treatment includes surgery, radiotherapy and chemotherapy, and in recent years, the traditional surgical excision treatment such as a freezing method, laser treatment and electrocoagulation method is provided, wherein the freezing method is only suitable for cases with small focus and low CIN level; relapse is easy after laser treatment; the electrocoagulation method has the defects of residual cancer focus, postoperative secondary bleeding, obvious pain and the like after treatment; the chemotherapy effect is not ideal, and the traditional Chinese medicine is mainly used for late-stage palliative treatment; the curative effect of radiotherapy is satisfactory but the reaction is large, especially the late reaction to rectum, which causes patients to have continuous hematochezia, anemia, weakness and listlessness to lose the working ability.

The relationship between the treatment of cervical cancer and the traditional Chinese medicine is very close, for example, in the aspect of synergy and detoxification, the traditional Chinese medicine applies the principle of strengthening body resistance and eliminating pathogenic factors to perform treatment based on syndrome differentiation, controls and prevents the metastasis of cancer to a certain extent, the traditional Chinese medicine resources in China are rich, and the research of searching for a high-efficiency cervical cancer treatment medicine from natural medicines is one of important subjects.

Disclosure of Invention

An object of the present invention is to solve at least the above problems and to provide at least the advantages described later.

The invention also aims to provide an external medicine for treating female cervical cancer, which has the effects of clearing heat and removing toxicity, strengthening body resistance and eliminating evil, promoting blood circulation to remove blood stasis and softening hardness to eliminate tumor by the synergistic effect of all the components, has good treatment effect on the cervical cancer, and has obvious effect.

The invention also aims to provide a preparation method of the external medicine for treating female cervical cancer, which takes 18 Chinese herbal medicines as raw materials, adopts virgin stock mature vinegar, and has no chemical and biochemical reactions in the production and preparation process, centrifugal separation and extraction, and the whole extraction and preparation process has pure physical behaviors, and the product does not contain any chemical components.

In order to achieve the objects and other advantages according to the present invention, an external medicine for actively treating female cervical cancer is provided, wherein the raw materials for preparing the external medicine comprise an L-40 amphiphilic transdermal agent, a traditional Chinese medicine composition and protoplasm mature vinegar in a mass ratio of 1:5-6, wherein the traditional Chinese medicine composition comprises the following components by weight: 38-42 parts of centipede, 42-47 parts of typhonium giganteum, 38-42 parts of angelica sinensis, 50-62 parts of brucea javanica, 63-67 parts of dysosma versipellis rhizome, 63-67 parts of sinopodium persicum, 38-42 parts of davidian, 68-72 parts of golden larch bark, 43-47 parts of nux vomica, 58-62 parts of cyrtomium rhizome, 43-47 parts of myrrh, 48-52 parts of zugushengfeng, 43-47 parts of polyrhachis vicina, 58-62 parts of quisqualis, 48-52 parts of pepper, 8-12 parts of leech, 8-12 parts of astragalus membranaceus and 13-17 parts of radix scrophulariae.

Preferably, the mass ratio of the traditional Chinese medicine composition to the original stock mature vinegar is 1: 5.5.

Preferably, the traditional Chinese medicine composition comprises the following components in parts by weight: 40 parts of centipede, 45 parts of typhonium giganteum, 40 parts of angelica sinensis, 60 parts of brucea javanica, 65 parts of dysosma versipellis roots and stems, 65 parts of persicaria segetum, 40 parts of davidia, 70 parts of golden larch bark, 45 parts of nux vomica, 60 parts of cyrtomium rhizome, 45 parts of myrrh, 50 parts of zucchini, 45 parts of polyrhachis vicina, 60 parts of rangooncreeper fruit, 50 parts of pepper, 10 parts of leech, 10 parts of astragalus membranaceus and 15 parts of radix scrophulariae.

A preparation method of an external medicine for treating female cervical cancer comprises the following steps:

preferably, the preparation method comprises the following steps of weighing centipede, typhonium giganteum, angelica, brucea javanica, dysosma versipellis rhizome, sinopodophyllum emodi, davidian pier, golden larch bark, nux vomica, cyrtomium rhizome, myrrh, roughhaired spurge, polyrhachis vicina, rangooncreeper fruit, pepper, leech, astragalus mongholicus and radix scrophulariae according to weight components, respectively crushing the materials, putting the materials into a ceramic jar together to obtain Chinese medicinal powder, adding virgin stock mature vinegar, sealing the mouth of the ceramic jar, and soaking for 55-75 days to obtain a soaking mixture;

step two, putting the soaked mixture into an iron container, decocting the soaked mixture by using a slow fire until the soaked mixture is dried to obtain juice, and synchronously controlling the volatilization loss rate of the raw stock mature vinegar to be 8-12% to obtain a decocted mixture;

step three, centrifugally filtering the decocted mixture to remove residues to obtain filtered medicine liquid, adding the L-40 amphiphilic transdermal agent after the temperature of the medicine liquid is reduced to 50-55 ℃, and uniformly stirring to obtain the medicine liquid, wherein the mass ratio of the L-40 amphiphilic transdermal agent to the filtered medicine liquid is 3.5-4.5%;

and step five, packaging the liquid medicine to obtain the traditional Chinese medicine.

Preferably, the soaking in step one is carried out for 60 days.

Preferably, the mass ratio of the L-40 amphiphilic transdermal agent to the filtered medicinal juice is 4%.

The invention at least comprises the following beneficial effects:

firstly, the external medicine takes 18 Chinese herbal medicines as raw materials, adopts original pulp mature vinegar, and is subjected to long-time soaking, warm fire decocting, centrifugal separation and extraction, the production and preparation process has no chemical and biochemical reaction, the whole extraction and preparation process has pure physical behavior, the product does not contain any chemical component, and no toxic or side effect is generated on liver, kidney and other organs after determination;

secondly, the application of the externally applied medicine is externally applied liquid for percutaneous absorption and local application, wherein the L-40 amphiphilic transdermal agent can overcome the barrier of the horny layer of the epidermis, so that the medicine can smoothly enter dermal tissues to reach subcutaneous fat and then is absorbed by capillary vessels in the dermal tissues to enter blood circulation, is not absorbed by the stomach, does not damage the liver, the gallbladder, the spleen and the kidney, does not cause burden on intestines and stomach, has no toxic or side effect, greatly improves the bioavailability of the medicine, reduces the dosage of the medicine, prolongs the medicine effect and shortens the treatment time, and is particularly suitable for patients with reduced absorption function of the spleen and the stomach;

thirdly, the external medicine has the efficacies of clearing away heat and toxic material, strengthening body resistance to eliminate pathogenic factors, promoting blood circulation to remove blood stasis and softening hardness to eliminate tumor by the synergistic effect of all the components, has good treatment effect on cervical cancer, and has obvious effect, wherein the polyrhachis vicina roger, the pepper, the astragalus mongholicus and the leech are matched to promote the circulation of qi and break the blood, and promote the circulation of qi and attack accumulation; removing stagnation and dredging channels; the angelica, the radix aristolochiae and the golden larch bark are matched with the functions of dredging collaterals, activating blood, dispelling damp toxin, detoxifying and promoting eruption; the six medicines of the nux vomica, the rangooncreeper fruit, the cyrtomium rhizome, the figwort root, the myrrh and the zugium leptostachya are mixed to play the roles of nourishing yin, tonifying liver and kidney, clearing away the lung-heat, moistening dryness, sterilizing, dispersing mass, detoxifying and diminishing swelling; astragalus root, centipede, typhonium giganteum, brucea javanica, dysosma versipellis rhizome and root of Tachouli root, and has the effects of clearing away heat and toxic materials, removing putrefaction and promoting granulation. According to the action principle of detoxification and stagnation, blood circulation promotion and blood stasis removal and balance, the traditional medicine is based on the skin theory of traditional Chinese medicine for female cervical cancer, and adopts the modes of internal disease external treatment, local application, transdermal absorption and direct focus, so that the medicine regulates the body functions through nerve conduction to form a strong and effective antibody, nourishes yin and tonifies kidney, clears lung and moistens dryness, decomposes and clears viruses in vivo, repairs and activates diseased cells to regenerate, and improves the division and growth of cells.

And fourthly, from the holistic concept, the treatment based on syndrome differentiation is implemented, the cervical cancer treatment method has important effects on local symptoms and the general conditions of a cervical cancer patient, the patient does not feel any discomfort and pain in the treatment period in the whole treatment period, the patient does not need to have thought preparation before medication treatment, the physiological and fertility functions of the patient are not influenced, the treatment method is simple and convenient, the lower body of the patient is more sanitary and clean, the side effects of radiotherapy, chemotherapy and operation can be reduced, and the defects of operation treatment, radiotherapy and chemotherapy of late-stage cancer patients are overcome.

Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.

Detailed Description

The present invention is further described in detail below with reference to examples so that those skilled in the art can practice the invention with reference to the description.

< example 1>

An external medicine for treating female cervical cancer is prepared from raw materials of an L-40 amphiphilic transdermal agent, a traditional Chinese medicine composition and protoplasm mature vinegar in a mass ratio of 1:5.5, wherein the traditional Chinese medicine composition comprises the following components in parts by weight: 40 parts of centipede, 45 parts of typhonium giganteum, 40 parts of angelica sinensis, 60 parts of brucea javanica, 65 parts of dysosma versipellis roots and stems, 65 parts of persicaria segetum, 40 parts of davidia, 70 parts of golden larch bark, 45 parts of nux vomica, 60 parts of cyrtomium rhizome, 45 parts of myrrh, 50 parts of zucchini, 45 parts of polyrhachis vicina, 60 parts of rangooncreeper fruit, 50 parts of pepper, 10 parts of leech, 10 parts of astragalus membranaceus and 15 parts of radix scrophulariae;

the preparation method of the external medicine for treating female cervical cancer comprises the following steps:

weighing 40 parts of centipede, 45 parts of typhonium giganteum, 40 parts of angelica sinensis, 60 parts of brucea javanica, 65 parts of dysosma versipellis roots, 65 parts of persicaria segetalis, 40 parts of davidia, 70 parts of golden larch barks, 45 parts of nux vomica, 60 parts of cyrtomium fortunei, 45 parts of myrrh, 50 parts of diamond-bone wind, 45 parts of polyrhachis vicina, 60 parts of rangooncreeper fruits, 50 parts of pepper, 10 parts of leeches, 10 parts of astragalus membranaceus and 15 parts of radix scrophulariae according to the weight parts, respectively crushing, putting the crushed materials into a ceramic jar together to obtain traditional Chinese medicine powder, adding protoplasm mature vinegar, sealing the mouth of the ceramic jar, and soaking for 60 days to obtain a soaking mixture, wherein 815 parts of the traditional Chinese medicine composition are added, and 4482.5 parts of the protoplasm mature vinegar are added according to;

step two, putting the soaked mixture into an iron container, decocting the soaked mixture by using a slow fire until the soaked mixture is dried to obtain juice, and synchronously controlling the volatilization loss rate of the raw stock mature vinegar to be within 8-12% to obtain a decocted mixture;

step three, centrifugally filtering the decocted mixture to remove residues to obtain filtered medicine liquid, adding the L-40 amphiphilic transdermal agent after the temperature of the medicine liquid is reduced to 50 ℃, and uniformly stirring to obtain the medicine liquid, wherein the mass ratio of the L-40 amphiphilic transdermal agent to the filtered medicine liquid is 4%;

step five, bottling the liquid medicine according to the net weight of 120mL of each bottle;

step six, packaging: 1 bottle/box, wherein each box is provided with 10 tampons.

< example 2>

An external medicine for treating female cervical cancer is prepared from raw materials of an L-40 amphiphilic transdermal agent, a traditional Chinese medicine composition and protoplasm mature vinegar in a mass ratio of 1:6, wherein the traditional Chinese medicine composition comprises the following components in parts by weight: 38 parts of centipede, 42 parts of typhonium giganteum, 38 parts of angelica sinensis, 50 parts of brucea javanica, 63 parts of dysosma versipellis roots and stems, 63 parts of stauntonvine, 38 parts of davidia, 68 parts of golden larch bark, 43 parts of nux vomica, 58 parts of cyrtomium rhizome, 43 parts of myrrh, 48 parts of zucchini, 43 parts of polyrhachis vicina, 58 parts of rangooncreeper fruit, 48 parts of pepper, 8 parts of leech, 8 parts of astragalus membranaceus and 13 parts of radix scrophulariae;

weighing 38 parts of centipede, 42 parts of typhonium giganteum, 38 parts of angelica sinensis, 50 parts of brucea javanica, 63 parts of dysosma versipellis roots, 63 parts of sinopodophyllum persicum, 38 parts of davidia, 68 parts of golden larch barks, 43 parts of nux vomica, 58 parts of cyrtomium fortunei, 43 parts of myrrh, 48 parts of diamond-bone wind, 43 parts of polyrhachis vicina, 58 parts of rangooncreeper fruit, 48 parts of pepper, 8 parts of leech, 8 parts of astragalus membranaceus and 13 parts of radix scrophulariae according to the weight parts, respectively crushing, putting into a ceramic jar together to obtain traditional Chinese medicine powder, adding virgin stock mature vinegar, sealing the mouth of the ceramic jar, and soaking for 55 days to obtain a soaking mixture, wherein 770 parts of the traditional Chinese medicine composition are added, and 4620 parts of the virgin stock vinegar are added according to the mass;

step three, centrifugally filtering the decocted mixture to remove residues to obtain filtered medicine liquid, adding the L-40 amphiphilic transdermal agent after the temperature of the medicine liquid is reduced to 52 ℃, and uniformly stirring to obtain the medicine liquid, wherein the mass ratio of the L-40 amphiphilic transdermal agent to the filtered medicine liquid is 3.5%;

< example 3>

An external medicine for treating female cervical cancer is prepared from raw materials of an L-40 amphiphilic transdermal agent, a traditional Chinese medicine composition and protoplasm mature vinegar in a mass ratio of 1:5, wherein the traditional Chinese medicine composition comprises the following components in parts by weight: 42 parts of centipede, 47 parts of typhonium giganteum, 42 parts of angelica sinensis, 62 parts of brucea javanica, 67 parts of dysosma versipellis roots and stems, 67 parts of persicaria segetum, 42 parts of davidia, 72 parts of golden larch bark, 47 parts of nux vomica, 62 parts of cyrtomium rhizome, 47 parts of myrrh, 52 parts of zucchini, 47 parts of polyrhachis vicina, 62 parts of rangooncreeper fruit, 52 parts of pepper, 12 parts of leech, 12 parts of astragalus membranaceus and 17 parts of radix scrophulariae;

weighing 42 parts of centipedes, 47 parts of typhonium giganteum, 42 parts of angelica sinensis, 62 parts of brucea javanica, 67 parts of dysosma versipellis roots, 67 parts of persicaria segetalis, 42 parts of davidia, 72 parts of golden larch barks, 47 parts of nux vomica, 62 parts of cyrtomium fortunei, 47 parts of myrrh, 52 parts of diamond-bone wind, 47 parts of polyrhachis vicina, 62 parts of rangooncreeper fruits, 52 parts of pepper, 12 parts of leeches, 12 parts of astragalus membranaceus and 17 parts of radix scrophulariae according to the weight parts, respectively crushing, putting into a ceramic jar together to obtain traditional Chinese medicine powder, adding virgin stock mature vinegar, sealing the mouth of the ceramic jar, and soaking for 75 days to obtain a soaking mixture, wherein 851 parts of the traditional Chinese medicine composition are added, and 4250 parts of the virgin stock vinegar are added according to the mass;

step three, centrifugally filtering the decocted mixture to remove residues to obtain filtered medicine liquid, adding the L-40 amphiphilic transdermal agent after the temperature of the medicine liquid is reduced to 55 ℃, and uniformly stirring to obtain the medicine liquid, wherein the mass ratio of the L-40 amphiphilic transdermal agent to the filtered medicine liquid is 4.5%;

The external medicine for treating female cervical cancer prepared in examples 1 to 3 is stored in a cool and dry place, and has a shelf life of 24 months, wherein pregnant women, lactating women, and women in menstrual period are prohibited from taking.

Research experiment

1 according to the technical Specification for safety of cosmetics 2015, the total number of bacterial colonies, the total number of moulds and yeasts, the total number of heat-resistant coliform bacteria, pseudomonas aeruginosa and staphylococcus aureus of the products of examples 1-3 are detected, and the specific results are shown in the following table 1:

TABLE 1

2 toxicity test

20 Kunming mice with the weight of 18-22g are selected, half of the female and half of the male are selected, after the Kunming mice are adapted to the environment for 4 days, are fasted for 16h and are randomly divided into 4 groups, namely an experimental group 1a (fed with the product of example 1), an experimental group 2a (fed with the product of example 2), an experimental group 3a (fed with the product of example 3) and a control group (fed with water), wherein the feeding amount of each mouse is 0.4mL/20g of the weight, the feeding is carried out for 3 times at an interval of 2h for two adjacent times, the mice are continuously observed for one week after the last gastric feeding, all the 20 mice are normal, and no abnormal phenomenon occurs.

3 multiple vaginal mucosa irritation test

Taking 8 New Zealand rabbits (1.5-1.8 kg), randomly dividing the rabbits into 4 groups (2 rabbits each), namely an experimental group 1b (example 1), an experimental group 2b (example 2), an experimental group 3b (example 3) and a control group;

injecting 1mL of the liquid medicine of the example 1 into the vagina of each rabbit of the experimental group 1, injecting 1mL of the liquid medicine of the example 2 into the vagina of each rabbit of the experimental group 2, injecting 1mL of the liquid medicine of the example 3 into the vagina of each rabbit of the experimental group 3, injecting 1mL of physiological saline into the vagina of each rabbit of the control group, continuously injecting for 6 days, injecting twice per day, and for the same value of rabbit, injecting any two times adjacently at intervals of at least 10 hours;

after 24 hours of the last injection, the rabbits were sacrificed by air embolism method, and two ends and the middle part of the vagina were selected respectively for histopathological observation, which indicates that the medicinal liquids of examples 1, 2 and 3 have no irritation to the vaginal mucosa.

4 study on the inhibition of tumor weight of transplanted tumors

4.1 cell culture

Culturing with DEME containing 10% calf serum at 37 deg.C under 5% CO₂And conventionally culturing human cervical cancer Hela cells in a cell culture box.

4.2 construction of animal model of transplanted tumor

Selecting 80 BALA/CA-nu nude mice, age 5 weeks, weight 15-18g, taking human cervical carcinoma Hela cells in logarithmic growth phase, injecting subcutaneously on back of nude mice, each injecting 1 x 10⁶One cell/one.

4.3 analysis of results

After inoculation, the growth of the tumor was observed every other week, and the major axis X and the minor axis Y of the tumor mass were measured with a vernier caliper, respectively, and the volume V of the tumor mass was calculated to be 1/2XY²The measurement shows that 2 mice die in the tumor growth process, the tumor size of the rest nude mice can not be measured by a vernier caliper at 1 week, the tumor enters the logarithmic growth phase at 2 weeks, and the volume of the implanted tumor under the skin on the 21 st day can reach 221mm³；

Dividing 78 nude tumor mice into 4 groups according to tumor volume balance principle, wherein three groups are 20, the three groups are respectively experiment group 1, experiment group 2 and experiment group 3, the other group is control group, and 18 mice are in total, wherein, the tumor forming part of the control group is coated with pre-treated physiological saline (3.5% of L-40 amphiphilic transdermal agent by mass is added into 5% of physiological saline, and the mixture is stirred and mixed uniformly); the tail of the experimental group 1 is coated with the liquid medicine of the example 1; the tail of the experimental group 2 is coated with the liquid medicine of the example 2; the tail of the experimental group 3 is coated with the liquid medicine in the embodiment 3; the medicine is administrated 2 times a day for 12 days in total, the mice are killed by dislocation after the last administration for 48 hours, transplanted tumors are excised, the tumor weight is weighed, and the following calculation is carried out: tumor weight inhibition (%) IR ═ 100% (1-tumor weight average of experimental group/tumor weight average of control group);

analysis of variance was performed using SPSS15.0 software, and the results are shown in table 2:

TABLE 2

As shown in the table 2 and combined observation, the external medicine for treating female cervical cancer can effectively inhibit the growth of the artificial cervical cancer Hela cell nude mouse transplantation tumor.

5 clinical trial

The experimenter: performing experimental determination on 44 patients diagnosed with cervical cancer, wherein 17 patients with early stage, 10 patients with middle stage, 13 patients with middle and late stage and 4 patients with late stage are selected from the 44 patients with cervical cancer;

diagnostic criteria: the inflammatory secretion in the vagina is more at the early stage of the cervical cancer, the squamous epithelium on the surface of the cervix is peeled off, and the contact bleeding is mostly at the early stage; irregular vaginal bleeding in the middle and late stages; necrosis and desquamation of late cancer tissues to form ulcer or holes with malodor, wherein benign cervical lesions correspond to early patients and specifically symptoms comprise cervical columnar epithelial dislocation, cervical polyp, cervical endometriosis, cervical tuberculous ulcer and the like; benign tumor of cervix is corresponding to the middle stage, the concrete symptom is myoma under cervical mucosa, cervical canal myoma, cervical papilloma, etc.; the cervical malignant tumor is a patient with middle and late stage, and has symptoms of primary malignant melanoma, sarcoma, lymphoma, metastatic cancer, etc.;

the test method comprises the following steps: the external medicine for treating female cervical cancer prepared in example 1 is used, 15 days are taken as one treatment course, 3 treatment courses are taken as one cycle, and the treatment course is carried out once in the morning and at the evening each day, wherein the use method comprises the following steps: before use, wearing gloves, cleaning vagina and air drying; the bottle cap with the liquid medicine is unscrewed, the cotton sliver is placed into the liquid medicine for soaking for 2-3 hours before use, the cotton sliver is taken out when in use, the cotton sliver rod is aligned with the vaginal opening and pushed into the deep part of the vaginal opening, and the cotton sliver rod is taken out and thrown away after being placed for 15-20 minutes.

The curative effect standard is as follows: the tumor is examined and measured in various conditions, the product of the maximum diameter and the maximum vertical diameter of the tumor represents the change and the curative effect before and after the tumor treatment, and the tumor can be divided into the following parts according to the absorption degree:

and (3) curing: the focus disappears completely;

basically curing: the focus is reduced by more than or equal to 50 percent;

and (3) relieving: the focus is reduced by more than 20 percent and less than 50 percent

And (4) invalidation: others

After one cycle of treatment, the treatment is as follows in table 3:

TABLE 3

	Cure of disease	Basic cure	Mitigation	Invalidation
					Example 1	19	14	9	2

Wherein 17 cases of early stage patients are completely cured, 2 cases of 10 cases of middle stage patients are cured, 8 cases of middle stage patients are basically cured, 6 cases of 13 cases of middle stage patients are basically cured, 7 cases of late stage patients are relieved, and 2 cases of 4 cases of late stage patients are relieved and 2 cases of late stage patients are invalid; namely, the external medicine for treating female cervical carcinoma has excellent treatment effect on cervical carcinoma, and mainly comprises the following components:

by means of the penetration of the effective components of the traditional Chinese medicine, the efficacy of the traditional Chinese medicine can quickly reach the focus directly, firstly, the inflammation process can be effectively blocked, the inflammatory reaction of viral factors can be effectively controlled, the yin is nourished, the kidney is nourished, the lung is cleared, the dryness is moistened and the sterilization is carried out, the stagnation is eliminated, the swelling is relieved, the decay is removed and the toxicity is removed, and after the traditional Chinese medicine is taken for 15 days, the cervical edema can be effectively relieved for a patient, the bleeding is reduced;

within 16-30 days of use, the immunity of cervical local tissues is enhanced, the morphological structure of cancer cells is transformed to benign, the ulcerated surface of the tumor is reduced, the internal system of the organism is gradually balanced, white or bloody liquid and fishy smell of the vagina disappear, and the effects of detoxifying, promoting eruption, removing stasis, relieving swelling, sterilizing, astringing wound surfaces, promoting granulation and activating blood circulation are achieved.

After 3 courses of treatment, pathological observation is carried out on the cervical biopsy tissues before and after 19 cases of patients with cervical cancer are cured, after treatment, the tumors are found to be coagulated, necrosed and shed, the morphological structures of the cervix and the cells are converted into normal, the immunologic function of the local tissues of the cervix is enhanced, the cancer cells are converted into benign, the normal cells are activated, and the division and growth of the cells are improved. Promoting regeneration of damaged fat cells, fibroblasts and interstitial cells, maintaining metabolism balance of cervical local tissue substances, and restoring organism balance and metabolism.

While embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable to various fields of endeavor for which the invention may be embodied with additional modifications as would be readily apparent to those skilled in the art, and the invention is therefore not limited to the details given herein and to the embodiments shown and described without departing from the generic concept as defined by the claims and their equivalents.

Claims

1. The external medicine for treating female cervical cancer is characterized by comprising L-40 amphiphilic transdermal agent, a traditional Chinese medicine composition and protoplasm mature vinegar in a mass ratio of 1:5-6, wherein the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 38-42 parts of centipede, 42-47 parts of typhonium giganteum, 38-42 parts of angelica sinensis, 50-62 parts of brucea javanica, 63-67 parts of dysosma versipellis rhizome, 63-67 parts of sinopodium persicum, 38-42 parts of davidian, 68-72 parts of golden larch bark, 43-47 parts of nux vomica, 58-62 parts of cyrtomium rhizome, 43-47 parts of myrrh, 48-52 parts of zugushengfeng, 43-47 parts of polyrhachis vicina, 58-62 parts of quisqualis, 48-52 parts of pepper, 8-12 parts of leech, 8-12 parts of astragalus membranaceus and 13-17 parts of radix scrophulariae.

2. The external medicine for treating female cervical cancer according to claim 1, wherein the mass ratio of the traditional Chinese medicine composition to the raw stock mature vinegar is 1: 5.5.

3. The external medicine for treating female cervical cancer according to claim 1, wherein the traditional Chinese medicine composition comprises the following components by weight: 40 parts of centipede, 45 parts of typhonium giganteum, 40 parts of angelica sinensis, 60 parts of brucea javanica, 65 parts of dysosma versipellis roots and stems, 65 parts of persicaria segetum, 40 parts of davidia, 70 parts of golden larch bark, 45 parts of nux vomica, 60 parts of cyrtomium rhizome, 45 parts of myrrh, 50 parts of zucchini, 45 parts of polyrhachis vicina, 60 parts of rangooncreeper fruit, 50 parts of pepper, 10 parts of leech, 10 parts of astragalus membranaceus and 15 parts of radix scrophulariae.

4. The method for preparing an external medicine for treating cervical cancer in women according to any one of claims 1 to 3, comprising the steps of:

weighing centipede, typhonium giganteum, angelica, brucea javanica, dysosma versipellis roots, sinopodophyllum emodi, davidianus, golden larch bark, nux vomica, cyrtomium rhizome, myrrh, roughhaired spurge, polyrhachis vicina, rangooncreeper fruit, pepper, leech, astragalus membranaceus and radix scrophulariae according to the weight components, respectively crushing the materials, putting the materials into a ceramic jar together to obtain Chinese medicinal powder, adding virgin stock mature vinegar, sealing the mouth of the ceramic jar, and soaking the Chinese medicinal powder for 55-75 days to obtain a soaking mixture;

5. The method of claim 4, wherein the first step of soaking for 60 days.

6. The method for preparing an external medicine for treating cervical cancer in women according to claim 4, wherein the mass ratio of the L-40 amphiphilic transdermal agent to the filtered juice is 4%.