CN110352965B - Pesticide composition containing chitosan oligosaccharide and brassinolide and application - Google Patents
Pesticide composition containing chitosan oligosaccharide and brassinolide and application Download PDFInfo
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- CN110352965B CN110352965B CN201910639584.XA CN201910639584A CN110352965B CN 110352965 B CN110352965 B CN 110352965B CN 201910639584 A CN201910639584 A CN 201910639584A CN 110352965 B CN110352965 B CN 110352965B
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- CN
- China
- Prior art keywords
- water
- brassinolide
- pesticide composition
- chitosan oligosaccharide
- pesticidal
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- 239000000203 mixture Substances 0.000 title claims abstract description 86
- 239000000575 pesticide Substances 0.000 title claims abstract description 50
- IXVMHGVQKLDRKH-VRESXRICSA-N Brassinolide Natural products O=C1OC[C@@H]2[C@@H]3[C@@](C)([C@H]([C@@H]([C@@H](O)[C@H](O)[C@H](C(C)C)C)C)CC3)CC[C@@H]2[C@]2(C)[C@@H]1C[C@H](O)[C@H](O)C2 IXVMHGVQKLDRKH-VRESXRICSA-N 0.000 title claims abstract description 33
- IXVMHGVQKLDRKH-KNBKMWSGSA-N brassinolide Chemical compound C1OC(=O)[C@H]2C[C@H](O)[C@H](O)C[C@]2(C)[C@H]2CC[C@]3(C)[C@@H]([C@H](C)[C@@H](O)[C@H](O)[C@@H](C)C(C)C)CC[C@H]3[C@@H]21 IXVMHGVQKLDRKH-KNBKMWSGSA-N 0.000 title claims abstract description 33
- RQFQJYYMBWVMQG-IXDPLRRUSA-N chitotriose Chemical compound O[C@@H]1[C@@H](N)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](N)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)N)[C@@H](CO)O1 RQFQJYYMBWVMQG-IXDPLRRUSA-N 0.000 title claims abstract description 31
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 31
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 25
- 238000002360 preparation method Methods 0.000 claims abstract description 24
- 239000003995 emulsifying agent Substances 0.000 claims abstract description 21
- JBTWLSYIZRCDFO-UHFFFAOYSA-N ethyl methyl carbonate Chemical compound CCOC(=O)OC JBTWLSYIZRCDFO-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000000126 substance Substances 0.000 claims abstract description 14
- 238000003756 stirring Methods 0.000 claims description 19
- 238000009472 formulation Methods 0.000 claims description 18
- -1 xylitol anhydride Chemical class 0.000 claims description 16
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 14
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 12
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 9
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 claims description 9
- 239000000811 xylitol Substances 0.000 claims description 9
- 229960002675 xylitol Drugs 0.000 claims description 9
- 235000010447 xylitol Nutrition 0.000 claims description 9
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 8
- 239000003795 chemical substances by application Substances 0.000 claims description 8
- ZSIAUFGUXNUGDI-UHFFFAOYSA-N hexan-1-ol Chemical compound CCCCCCO ZSIAUFGUXNUGDI-UHFFFAOYSA-N 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 8
- YYZUSRORWSJGET-UHFFFAOYSA-N octanoic acid ethyl ester Natural products CCCCCCCC(=O)OCC YYZUSRORWSJGET-UHFFFAOYSA-N 0.000 claims description 7
- 239000000080 wetting agent Substances 0.000 claims description 7
- 229940051841 polyoxyethylene ether Drugs 0.000 claims description 6
- 229920000056 polyoxyethylene ether Polymers 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 5
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 4
- KBPLFHHGFOOTCA-UHFFFAOYSA-N caprylic alcohol Natural products CCCCCCCCO KBPLFHHGFOOTCA-UHFFFAOYSA-N 0.000 claims description 4
- 230000001804 emulsifying effect Effects 0.000 claims description 4
- QPJVMBTYPHYUOC-UHFFFAOYSA-N methyl benzoate Chemical compound COC(=O)C1=CC=CC=C1 QPJVMBTYPHYUOC-UHFFFAOYSA-N 0.000 claims description 4
- 239000011265 semifinished product Substances 0.000 claims description 4
- 239000000243 solution Substances 0.000 claims description 4
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- ZORQXIQZAOLNGE-UHFFFAOYSA-N 1,1-difluorocyclohexane Chemical compound FC1(F)CCCCC1 ZORQXIQZAOLNGE-UHFFFAOYSA-N 0.000 claims description 2
- HNAGHMKIPMKKBB-UHFFFAOYSA-N 1-benzylpyrrolidine-3-carboxamide Chemical compound C1C(C(=O)N)CCN1CC1=CC=CC=C1 HNAGHMKIPMKKBB-UHFFFAOYSA-N 0.000 claims description 2
- RMTFNDVZYPHUEF-XZBKPIIZSA-N 3-O-methyl-D-glucose Chemical compound O=C[C@H](O)[C@@H](OC)[C@H](O)[C@H](O)CO RMTFNDVZYPHUEF-XZBKPIIZSA-N 0.000 claims description 2
- 229920001661 Chitosan Polymers 0.000 claims description 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims description 2
- 150000008064 anhydrides Chemical class 0.000 claims description 2
- OBNCKNCVKJNDBV-UHFFFAOYSA-N butanoic acid ethyl ester Natural products CCCC(=O)OCC OBNCKNCVKJNDBV-UHFFFAOYSA-N 0.000 claims description 2
- JMGZBMRVDHKMKB-UHFFFAOYSA-L disodium;2-sulfobutanedioate Chemical compound [Na+].[Na+].OS(=O)(=O)C(C([O-])=O)CC([O-])=O JMGZBMRVDHKMKB-UHFFFAOYSA-L 0.000 claims description 2
- 239000012153 distilled water Substances 0.000 claims description 2
- 239000008235 industrial water Substances 0.000 claims description 2
- 229940095102 methyl benzoate Drugs 0.000 claims description 2
- TVMXDCGIABBOFY-UHFFFAOYSA-N n-Octanol Natural products CCCCCCCC TVMXDCGIABBOFY-UHFFFAOYSA-N 0.000 claims description 2
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 claims description 2
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 claims description 2
- 229920005552 sodium lignosulfonate Polymers 0.000 claims description 2
- 239000001593 sorbitan monooleate Substances 0.000 claims description 2
- 235000011069 sorbitan monooleate Nutrition 0.000 claims description 2
- 229940035049 sorbitan monooleate Drugs 0.000 claims description 2
- ZQTYRTSKQFQYPQ-UHFFFAOYSA-N trisiloxane Chemical compound [SiH3]O[SiH2]O[SiH3] ZQTYRTSKQFQYPQ-UHFFFAOYSA-N 0.000 claims description 2
- 230000000361 pesticidal effect Effects 0.000 claims 15
- 238000007865 diluting Methods 0.000 claims 2
- WJTCHBVEUFDSIK-NWDGAFQWSA-N (2r,5s)-1-benzyl-2,5-dimethylpiperazine Chemical compound C[C@@H]1CN[C@@H](C)CN1CC1=CC=CC=C1 WJTCHBVEUFDSIK-NWDGAFQWSA-N 0.000 claims 1
- YENLEHMSMBFUTB-UHFFFAOYSA-N 2-octan-2-ylphenol Chemical compound CCCCCCC(C)C1=CC=CC=C1O YENLEHMSMBFUTB-UHFFFAOYSA-N 0.000 claims 1
- DUIOKRXOKLLURE-UHFFFAOYSA-N 2-octylphenol Chemical compound CCCCCCCCC1=CC=CC=C1O DUIOKRXOKLLURE-UHFFFAOYSA-N 0.000 claims 1
- TVACALAUIQMRDF-UHFFFAOYSA-N dodecyl dihydrogen phosphate Chemical compound CCCCCCCCCCCCOP(O)(O)=O TVACALAUIQMRDF-UHFFFAOYSA-N 0.000 claims 1
- 235000019333 sodium laurylsulphate Nutrition 0.000 claims 1
- 230000016615 flocculation Effects 0.000 abstract description 8
- 238000005189 flocculation Methods 0.000 abstract description 8
- 230000035699 permeability Effects 0.000 abstract description 3
- 230000005669 field effect Effects 0.000 abstract description 2
- 230000000052 comparative effect Effects 0.000 description 15
- 230000012010 growth Effects 0.000 description 10
- 239000003814 drug Substances 0.000 description 9
- 230000000694 effects Effects 0.000 description 9
- 240000008067 Cucumis sativus Species 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 7
- 240000008042 Zea mays Species 0.000 description 7
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 7
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 7
- 235000005822 corn Nutrition 0.000 description 7
- 239000000843 powder Substances 0.000 description 6
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 5
- 239000005980 Gibberellic acid Substances 0.000 description 5
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 5
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical compound C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 description 5
- 239000005648 plant growth regulator Substances 0.000 description 5
- 235000013399 edible fruits Nutrition 0.000 description 4
- 238000005507 spraying Methods 0.000 description 4
- 208000003643 Callosities Diseases 0.000 description 3
- 206010020649 Hyperkeratosis Diseases 0.000 description 3
- 230000001737 promoting effect Effects 0.000 description 3
- 235000009849 Cucumis sativus Nutrition 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 150000001298 alcohols Chemical class 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- 239000002569 water oil cream Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 235000004936 Bromus mango Nutrition 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 240000008436 Ipomoea aquatica Species 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 240000007228 Mangifera indica Species 0.000 description 1
- 235000014826 Mangifera indica Nutrition 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 235000001601 Sabal palmetto Nutrition 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 235000009184 Spondias indica Nutrition 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 235000010726 Vigna sinensis Nutrition 0.000 description 1
- 244000042314 Vigna unguiculata Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 241000219094 Vitaceae Species 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 239000012752 auxiliary agent Substances 0.000 description 1
- 244000000005 bacterial plant pathogen Species 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- LQZZUXJYWNFBMV-UHFFFAOYSA-N dodecan-1-ol Chemical compound CCCCCCCCCCCCO LQZZUXJYWNFBMV-UHFFFAOYSA-N 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000003759 ester based solvent Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000013022 formulation composition Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 235000021021 grapes Nutrition 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000012669 liquid formulation Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000004530 micro-emulsion Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000007762 w/o emulsion Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
Landscapes
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Dispersion Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a pesticide composition containing chitosan oligosaccharide and brassinolide and application, wherein the pesticide composition contains the chitosan oligosaccharide, the brassinolide, a water-soluble alcohol substance, an emulsifier and an ester solvent, wherein the mass ratio of the water-soluble alcohol substance to the emulsifier to the ester solvent is 1-5: 6-20: 30-60. The pesticide composition has excellent physical and chemical properties, and is characterized by not generating flocculation phenomenon when being tank-mixed with other preparation components. In addition, the pesticide composition has better permeability and shows better field effect after being diluted and sprayed by adding water subsequently.
Description
Technical Field
The invention relates to the field of agriculture, in particular to a pesticide composition containing chitosan oligosaccharide and brassinolide and application thereof.
Background
Chitosan oligosaccharide and brassinolide are common compound varieties appearing in recent years. The chitosan oligosaccharide can effectively improve the plant immunity, thereby improving the plant resistance to plant pathogenic bacteria. The brassinolide has multiple functions of rooting, promoting growth, lifting seedlings, strengthening seedlings, protecting flowers and fruits, promoting fruit expansion and precocity, enhancing stress resistance of crops and the like.
Chinese patent application CN101305738A discloses an agricultural mixture containing chitosan oligosaccharide and plant hormone containing brassinolide. The mixed agent is suitable for grain crops, fruit trees and the like, and can promote the yield of plants to be increased.
Chinese patent application CN107711844A discloses a composition containing amino-oligosaccharins and a plant growth regulator, wherein the plant growth regulator is selectable brassinolide. The composition expands the application range of single dosage of amino-oligosaccharin and plant growth regulator, effectively improves the drug effect of the single dosage, and promotes the growth of crops.
Chinese patent application CN104430354A discloses a biological pesticide with immune induced resistance effect, the effective components of which comprise 0.2-12 parts of amino-oligosaccharin and 1-20 parts of brassinolide by weight. The biological pesticide shows synergistic effect in a certain proportion range, and the sterilizing effect of the composition is improved compared with that of a single agent. The biological pesticide can be prepared into water aqua, microemulsion, water suspending agent and the like.
Chinese patent application CN102893991A discloses a plant growth regulator composition, which uses brassinolide and amino-oligosaccharin as effective components; the combination of the two active ingredients has obvious synergistic effect, can prevent and treat some fungi, bacteria and virus diseases of crops while improving the yield of the crops, and enhances the disease resistance and the stress resistance. The composition can be prepared into water aqua, soluble powder, water soluble granules and the like.
However, agricultural formulations containing chitosan oligosaccharide and brassinolide developed in the prior art have certain defects in application, such as relatively poor permeability, low utilization rate of active ingredients and undesirable effect. In addition, flocculation is more likely to occur when tank-mixed with other preparations. In actual production, there is an objective need for developing agricultural formulations with better application performance.
Disclosure of Invention
In order to overcome the defects of the prior art, the inventor finds that the chitosan oligosaccharide and the brassinolide are matched with a proper auxiliary agent to prepare a water-in-oil preparation, and the water-in-oil preparation shows a good field plant regulation effect after being diluted and sprayed with water subsequently. In addition, the problem that flocculation is easily generated when chitosan oligosaccharide and brassinolide other preparations are mixed in a tank in the prior art is solved. Based on this finding, the inventors have completed the present invention.
In order to solve the technical problems, the invention provides a pesticide composition containing chitosan oligosaccharide and brassinolide, which is characterized by comprising the chitosan oligosaccharide, the brassinolide, a water-soluble alcohol substance, an emulsifier and an ester solvent, wherein the mass ratio of the water-soluble alcohol substance to the emulsifier to the ester solvent is 1-5: 6-20: 30-60.
Preferably, in the pesticide composition, the mass ratio of the chitosan oligosaccharide to the brassinolide is 2-10: 0.001-0.006 wt% of chitosan oligosaccharide and brassinolide in the pesticide composition, preferably 2-11 wt%.
Preferably, in the pesticide composition, the sum of the weights of the water-soluble alcohol substance, the emulsifier and the ester solvent accounts for 25-80% of the weight of the pesticide composition. Preferably, the weight sum of the water-soluble alcohol substance, the emulsifier and the ester solvent accounts for 25-80% of the weight of the pesticide composition.
Preferably, in the pesticide composition, the water-soluble alcohol substance is selected from one or more of ethanol, propanol, isopropanol, n-butanol, n-hexanol and n-octanol; preferably, the water-soluble alcohol is ethanol, n-butanol or n-hexanol.
The existence of water-soluble alcohols can easily liquefy the organic solvent-emulsifier-water interfacial film, so that the interfacial film has high flexibility, is easy to bend, influences the spontaneous bending direction of the film and promotes the formation of W/O emulsion.
Preferably, in the pesticide composition, the emulsifier is one or more selected from the group consisting of polyoxyethylene octylphenol ether, polyoxyethylene sec-octylphenol ether, sorbitan monooleate, methyl glucose anhydride stearate and xylitol anhydride monooleate. Preferably, the emulsifier is polyoxyethylene octylphenol ether and/or xylitol anhydride monooleate. More preferably, the emulsifier is a mixture of polyoxyethylene octylphenol ether and xylitol anhydride monooleate, and the weight ratio of the polyoxyethylene octylphenol ether to the xylitol anhydride monooleate is 1: 1-3, preferably 1: 2.
in the invention, the emulsifiers have lower HLB value and more lipophilicity than hydrophilicity, so that W/O type emulsion is easier to form.
Preferably, in the pesticide composition, the ester solvent is selected from one or more of ethyl acetate, ethyl butyrate, ethyl octanoate and methyl benzoate. Preferably, the ester solvent is selected from ethyl acetate or ethyl octanoate.
The preferred mixtures of water-soluble alcohols, emulsifiers and ester solvents of the present invention are highly advantageous for forming the W/O emulsions of the present invention, and therefore mixtures of these adjuvants and/or solvents are most preferred in the present invention.
Preferably, the pesticide composition further comprises a wetting agent, wherein the wetting agent is selected from one or more of trisiloxane polyoxyethylene ether, sodium lignosulfonate, sodium dodecyl sulfate, sodium sulfosuccinate and lauryl alcohol phosphate, and the wetting agent accounts for 0.5-5% of the pesticide composition by weight.
Preferably, the pesticide composition further comprises water, wherein the water is deionized water, distilled water, industrial water and the like, and the water accounts for 5-40% of the pesticide composition by weight.
The invention also provides a preparation method of the pesticide composition, which comprises the following steps:
(1) adding chitosan oligosaccharide into water, stirring to dissolve chitosan oligosaccharide completely, and stirring to obtain chitosan oligosaccharide water solution;
(2) adding brassinolide into a liquid mixture of a water-soluble alcohol substance, an emulsifier and an ester solvent, and fully stirring to form a brassinolide single-agent semi-finished product;
(3) fully mixing and emulsifying the chitosan oligosaccharide aqueous solution and the brassinolide single-dose semi-finished product under the stirring condition to prepare the chitosan oligosaccharide-brassinolide pesticide composition.
Preferably, the stirring conditions are: adopting a rotary emulsifying machine at the rotating speed of 800-2000r/min, preferably 1000r/min until the oil-water emulsion is completely emulsified, and continuously stirring for 5-10min to obtain the stable pesticide composition, wherein the pesticide composition is a W/O type liquid preparation.
The invention also provides the use of the pesticide composition, which is characterized in that the use is used for reducing or eliminating flocs generated when the pesticide composition is mixed with other pesticide preparations in a tank.
Preferably, in the above application, the pesticide composition and other pesticide preparations are mixed in a preparation stage and then diluted, or are directly diluted respectively and then subjected to tank mixing.
The invention also provides a using method of the pesticide composition in tank mixing, which is characterized in that the pesticide composition is diluted and then added with other pesticide preparations, or the pesticide composition is mixed with other pesticide preparations and then diluted, or the pesticide composition and other preparations are respectively diluted and then tank mixed.
The invention has the advantages of
The pesticide composition is prepared by matching chitosan oligosaccharide and brassinolide with proper auxiliaries. On one hand, the W/O type preparation does not generate flocculation phenomenon when being mixed with other preparation components in a tank, and has stable physical properties, and other dosage forms such as chitosan oligosaccharide and brassinolide aqueous solution and the like are easy to generate flocculation phenomenon when being mixed with other preparation components in a tank, thereby influencing the product effect. On the other hand, the W/O type preparation has better permeability and shows better field effect after being diluted and sprayed by adding water.
The W/O type liquid preparation of the invention is suitable for various plants, including food crops, vegetables, fruit trees and the like, such as cucumbers, cowpeas, tomatoes, Shanghai green, swamp cabbage, wheat, corns, rice, soybeans, grapes, mangoes and the like, in particular cucumbers and corns. The dosage and the application times can be determined according to the growth vigor of crops, weather conditions and other factors, and can be easily determined by a person skilled in the art.
Detailed Description
The present invention is further illustrated by the following examples, which are not intended to limit the invention to these embodiments.
In the following examples of the present invention, "%" refers to weight percent unless otherwise specified.
First, preparation examples
The formulations of examples 1 to 6 according to the invention and comparative examples 1 to 3 are shown in the table below.
Table 1 formulation composition of formulations comprising chitosan oligosaccharide and brassinolide
The above examples and comparative examples 1 to 3 were prepared by the following method: firstly, the chitosan oligosaccharide with the formula amount is stirred with deionized water to be completely dissolved, and the stirring is continued to be uniform, so as to prepare a water phase. Then, adding the brassinolide, the water-soluble alcohol, the emulsifier and the optional wetting agent into the ester solvent according to the formula amount, stirring to completely dissolve the brassinolide, the water-soluble alcohol, the emulsifier and the optional wetting agent, and continuously stirring uniformly to obtain an oil phase. And then, adding the water phase into the oil phase while stirring at the rotating speed of 1000r/min by adopting a rotary emulsifying machine until the oil-water emulsion is completely emulsified, and continuously stirring for 5min to obtain the W/O type liquid preparation. Comparative example 3 was prepared by directly adding the ingredients to water, stirring well and continuing stirring for 5 min.
Second, field test
1. Influence on growth of cucumber seedlings
The test is carried out on cucumber seedlings, a field block is divided into a plurality of cells, and each cell has 20 square meters. The medicaments of examples 1 to 6 and the medicaments of comparative examples 1 to 3 are diluted by clear water to 500 times of liquid for spraying treatment, the spraying amount is controlled to be 30L per mu, each treatment is repeated for 3 times, and the same amount of clear water is sprayed to serve as a blank control. And 5 days after treatment, counting the plant height, the leaf number and the leaf area of each cucumber seedling in each cell by adopting a 5-point sampling method, and calculating the average value of each treatment. The results are shown in Table 2 below.
TABLE 2 Effect of the Agents of the present invention on cucumber seedling growth
| Treatment of | Plant height (cm) | Number of blades (sheet) | Leaf area (cm2) |
| Example 1 | 9.16 | 4.3 | 40.66 |
| Example 2 | 9.44 | 4.1 | 42.34 |
| Example 3 | 8.96 | 4.2 | 42.68 |
| Example 4 | 9.07 | 4.3 | 39.82 |
| Example 5 | 10.83 | 4.9 | 47.73 |
| Example 6 | 10.34 | 4.7 | 44.77 |
| Comparative example 1 | 7.18 | 3.8 | 32.53 |
| Comparative example 2 | 7.62 | 3.7 | 34.58 |
| Comparative example 3 | 6.88 | 3.5 | 29.92 |
| Blank control | 4.39 | 3.0 | 17.54 |
The results show that the effect of the medicament of the invention on promoting the growth of cucumber seedlings is obviously better than that of a control medicament. Among these, the formulations prepared in examples 5 to 6, in particular, showed the best results, with each index being clearly superior to the comparative examples.
2. Effect on corn growth
The test is carried out on the corn, and a field block is divided into a plurality of cells, wherein each cell has 20 square meters. The medicaments of examples 1 to 6 and the medicaments of comparative examples 1 to 3 are diluted by clear water to 500 times of liquid for spraying treatment, the spraying amount is controlled to be 30L per mu, each treatment is repeated for 3 times, and the same amount of clear water is sprayed to serve as a blank control. And (4) counting the growth vigor of the corns in each cell by adopting a 5-point sampling method after 7 days of treatment, and calculating an average value in each treatment. The results are given in Table 3 below.
TABLE 3 Effect of the Agents of the present invention on corn growth
The results show that the promotion effect of the medicament on the growth of the corn is obviously better than that of a control medicament. Among these, the formulations prepared in examples 5 to 6, in particular, showed the best results, with each index being clearly superior to the comparative examples.
Three, barrel mixing test
Since plant growth regulator species generally require mixed application, the formulation of the present invention was tested in a tank mix with a commercially available 20% gibberellic acid soluble powder. In particular to the flocculation condition of the drug in 500 times of liquid. The test procedure was as follows:
measuring 1L of clear water, adding 1ml of the formula preparation of the invention or the formula preparation of the comparative example, uniformly stirring, adding 1g of 20% gibberellic acid soluble powder, uniformly stirring, standing for 5min, and observing the appearance. Alternatively, the dosing sequence is reversed, i.e. the 20% gibberellic acid soluble powder is added first, followed by the formulation of the present invention or the formulation of the comparative example.
Note: "appearance 1" is the addition of the inventive or comparative formulation followed by the addition of the commercially available 20% gibberellic acid soluble powder;
"appearance 2" is the addition of commercially available 20% gibberellic acid soluble powder followed by the formulation of the present invention or the comparative formulation;
"+" indicates the severity of flocculation, with greater numbers being more severe;
from the above results, it can be seen that the W/O type liquid formulation of the present invention still shows good stability and compatibility in tank-mixing with other agents, while the comparative agent shows flocculation to various degrees. The W/O type liquid preparation of the present invention is preferable in practical use.
The above examples are merely illustrative and not restrictive of the present invention, and the solution obtained by simple modification of the above solution is also within the scope of the present application.
Claims (10)
1. A pesticide composition containing chitosan oligosaccharide and brassinolide is characterized by comprising the chitosan oligosaccharide, the brassinolide, a water-soluble alcohol substance, an emulsifier and an ester solvent, wherein the mass ratio of the water-soluble alcohol substance to the emulsifier to the ester solvent is 1-5: 6-20: 30-60, wherein the mass ratio of the chitosan oligosaccharide to the brassinolide is 2-10: 0.001-0.006 percent, wherein the sum of the weight of the chitosan oligosaccharide and the brassinolide accounts for 1-30 percent of the weight of the pesticide composition, and the sum of the weight of the water-soluble alcohol substance, the emulsifier and the ester solvent accounts for 25-80 percent of the weight of the pesticide composition, wherein the water-soluble alcohol substance is selected from one or more of ethanol, propanol, isopropanol, n-butanol, n-hexanol and n-octanol, the emulsifier is selected from one or more of octyl phenol polyoxyethylene ether, sec-octyl phenol polyoxyethylene ether, sorbitan monooleate, methyl glucose anhydride stearate and xylitol anhydride monooleate, the ester solvent is selected from one or more of ethyl acetate, ethyl butyrate, ethyl caprylate and methyl benzoate, and the preparation method of the pesticide composition comprises the following steps:
(1) adding chitosan oligosaccharide into water, stirring to dissolve chitosan oligosaccharide completely, and stirring to obtain chitosan oligosaccharide water solution;
(2) adding brassinolide into a liquid mixture of a water-soluble alcohol substance, an emulsifier and an ester solvent, and fully stirring to form a brassinolide single-agent semi-finished product;
(3) fully mixing and emulsifying the chitosan oligosaccharide aqueous solution and the brassinolide single-dose semi-finished product under the stirring condition to prepare the chitosan oligosaccharide-brassinolide pesticide composition.
2. The pesticidal composition according to claim 1, characterized in that the water-soluble alcohol is ethanol, n-butanol or n-hexanol.
3. The pesticidal composition according to claim 1, characterized in that the emulsifier is octylphenol polyoxyethylene ether and/or xylitol anhydride monooleate.
4. The pesticide composition as set forth in claim 3, characterized in that the emulsifier is a mixture of polyoxyethylene octylphenol ether and monooleate xylitol anhydride, and the weight ratio of polyoxyethylene octylphenol ether to monooleate xylitol anhydride is 1: 1-3.
5. The pesticidal composition according to claim 4, characterized in that the weight ratio of the octylphenol polyoxyethylene ether to the xylitol anhydride monooleate is 1: 2.
6. the pesticidal composition according to claim 1, wherein the ester solvent is selected from ethyl acetate or ethyl octanoate.
7. The pesticide composition as set forth in claim 1, characterized in that the pesticide composition further comprises a wetting agent selected from one or more of trisiloxane polyoxyethylene ether, sodium lignosulfonate, sodium lauryl sulfate, sodium sulfosuccinate and lauryl phosphate, the wetting agent is 0.5-5% by weight of the pesticide composition, the pesticide composition further comprises water, the water is deionized water, distilled water or industrial water, and the amount of the water is 5-40% by weight of the pesticide composition.
8. Use of a pesticidal composition according to any one of claims 1 to 7, characterised in that it is for reducing or eliminating flocs produced when tank-mixed with other pesticidal formulations.
9. The use of the pesticidal composition according to claim 8, characterized in that the pesticidal composition is tank-mixed with other pesticidal formulations by mixing at the formulation stage and then diluting, or by directly diluting separately and then tank-mixing.
10. The method for using the pesticidal composition according to any one of claims 1 to 7 in tank mix, characterized in that the pesticidal composition according to any one of claims 1 to 7 is diluted and then added to another pesticidal formulation, or the pesticidal composition is mixed with another pesticidal formulation and then diluted, or the pesticidal composition according to any one of claims 1 to 7 and the other formulation are diluted separately and then tank mixed.
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Denomination of invention: The invention relates to a pesticide composition comprising chitosan oligosaccharide and Brassinolide and its application Effective date of registration: 20220304 Granted publication date: 20220201 Pledgee: Bank of Hainan Limited by Share Ltd. Pledgor: HAINAN ZHENGYE ZHONGNONG HIGH TECHNOLOGY Co.,Ltd. Registration number: 2019460000007 |
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