CN110343191A - The extraction of Funiu Shan Mountain Dendrobium nobile polysaccharide and the method for preparing solid beverage - Google Patents
The extraction of Funiu Shan Mountain Dendrobium nobile polysaccharide and the method for preparing solid beverage Download PDFInfo
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- CN110343191A CN110343191A CN201910796601.0A CN201910796601A CN110343191A CN 110343191 A CN110343191 A CN 110343191A CN 201910796601 A CN201910796601 A CN 201910796601A CN 110343191 A CN110343191 A CN 110343191A
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- 150000004676 glycans Chemical class 0.000 title claims abstract description 64
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 64
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 64
- 240000004638 Dendrobium nobile Species 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 38
- 239000007787 solid Substances 0.000 title claims abstract description 19
- 235000013361 beverage Nutrition 0.000 title claims abstract description 18
- 238000000605 extraction Methods 0.000 title claims abstract description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 24
- 235000009508 confectionery Nutrition 0.000 claims abstract description 16
- 230000001376 precipitating effect Effects 0.000 claims abstract description 15
- 150000003384 small molecules Chemical class 0.000 claims abstract description 11
- 238000004042 decolorization Methods 0.000 claims abstract description 7
- 238000002360 preparation method Methods 0.000 claims abstract description 6
- 229960000935 dehydrated alcohol Drugs 0.000 claims abstract description 4
- 239000007788 liquid Substances 0.000 claims description 20
- 230000008569 process Effects 0.000 claims description 16
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 12
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 12
- 108090000790 Enzymes Proteins 0.000 claims description 11
- 102000004190 Enzymes Human genes 0.000 claims description 11
- 229940088598 enzyme Drugs 0.000 claims description 11
- 235000019441 ethanol Nutrition 0.000 claims description 11
- 239000000243 solution Substances 0.000 claims description 11
- 238000003756 stirring Methods 0.000 claims description 11
- 239000006228 supernatant Substances 0.000 claims description 10
- 238000002137 ultrasound extraction Methods 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 9
- 230000009849 deactivation Effects 0.000 claims description 9
- 229960004756 ethanol Drugs 0.000 claims description 8
- 239000012153 distilled water Substances 0.000 claims description 7
- 239000003153 chemical reaction reagent Substances 0.000 claims description 6
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 6
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 6
- 238000003760 magnetic stirring Methods 0.000 claims description 6
- 238000012545 processing Methods 0.000 claims description 6
- 239000002516 radical scavenger Substances 0.000 claims description 6
- 238000004108 freeze drying Methods 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 229920000858 Cyclodextrin Polymers 0.000 claims description 3
- 229920002774 Maltodextrin Polymers 0.000 claims description 3
- 239000005913 Maltodextrin Substances 0.000 claims description 3
- 108090000526 Papain Proteins 0.000 claims description 3
- 239000004365 Protease Substances 0.000 claims description 3
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 claims description 3
- 238000004090 dissolution Methods 0.000 claims description 3
- 238000001976 enzyme digestion Methods 0.000 claims description 3
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 3
- 239000010931 gold Substances 0.000 claims description 3
- 229910052737 gold Inorganic materials 0.000 claims description 3
- 235000021552 granulated sugar Nutrition 0.000 claims description 3
- 229940035034 maltodextrin Drugs 0.000 claims description 3
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 claims description 3
- 239000011259 mixed solution Substances 0.000 claims description 3
- 229940055729 papain Drugs 0.000 claims description 3
- 235000019834 papain Nutrition 0.000 claims description 3
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 claims description 3
- 235000000346 sugar Nutrition 0.000 claims description 3
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 claims description 3
- 229960002675 xylitol Drugs 0.000 claims description 3
- 235000010447 xylitol Nutrition 0.000 claims description 3
- 239000000811 xylitol Substances 0.000 claims description 3
- 241001523681 Dendrobium Species 0.000 claims description 2
- 238000000502 dialysis Methods 0.000 claims 1
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- QWUWMCYKGHVNAV-UHFFFAOYSA-N 1,2-dihydrostilbene Chemical group C=1C=CC=CC=1CCC1=CC=CC=C1 QWUWMCYKGHVNAV-UHFFFAOYSA-N 0.000 description 1
- 241000208340 Araliaceae Species 0.000 description 1
- 206010008132 Cerebral thrombosis Diseases 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 240000008397 Ganoderma lucidum Species 0.000 description 1
- 235000001637 Ganoderma lucidum Nutrition 0.000 description 1
- 201000001429 Intracranial Thrombosis Diseases 0.000 description 1
- 241001646834 Mesona Species 0.000 description 1
- 241001248610 Ophiocordyceps sinensis Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical class [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 1
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- 239000003146 anticoagulant agent Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 231100000433 cytotoxic Toxicity 0.000 description 1
- 230000001472 cytotoxic effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000003073 embolic effect Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000002024 ethyl acetate extract Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000004438 eyesight Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 150000002596 lactones Chemical class 0.000 description 1
- -1 lipid peroxide Chemical class 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004089 microcirculation Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000001730 monoaminergic effect Effects 0.000 description 1
- 210000003887 myelocyte Anatomy 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 208000013371 ovarian adenocarcinoma Diseases 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 201000006588 ovary adenocarcinoma Diseases 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000001953 sensory effect Effects 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/385—Concentrates of non-alcoholic beverages
- A23L2/39—Dry compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
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- Health & Medical Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Nutrition Science (AREA)
- Molecular Biology (AREA)
- Food Science & Technology (AREA)
- Materials Engineering (AREA)
- Sustainable Development (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
- Non-Alcoholic Beverages (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The extracting method of Funiu Shan Mountain Dendrobium nobile polysaccharide, comprising the following steps: (1) preparation of Funiu Shan Mountain HERBA DENDROBII Thick many candies；(2) albumen removal is handled；(3) decolorization；(4) small molecule removal is handled；(5) the Thick many candies solution for having removed small molecule is added into dehydrated alcohol and adjusts concentration of alcohol 70%, 4 DEG C stand overnight, and 15 min are centrifuged at 8000 r/min, collect precipitating, Funiu Shan Mountain Dendrobium nobile polysaccharide after being optimized.The present invention has operating method simple, is convenient for industrialized mass production, and the extraction content and purity, product quality for improving Funiu Shan Mountain Dendrobium nobile polysaccharide greatly improve, and prepares solid beverage with the polysaccharide, helps to improve the economic value of Funiu Shan Mountain HERBA DENDROBII.
Description
Technical field
The invention belongs to natural medicinal plant effective component extracting technical fields, and in particular to a kind of Funiu Shan Mountain HERBA DENDROBII
The extraction of polysaccharide and the method for preparing solid beverage.
Background technique
HERBA DENDROBII occupies one of big mesona of Chinese medicine nine, is one of the orchid recorded earliest in China's ancient documents.
It is just on the books in pervious Shennong's Herbals in 1500.Since thousand it always and ganoderma lucidum, ginseng, the same quilt such as cordyceps sinensis
It is classified as top grade Chinese medicine.Volt-second product is the natural treasure-house of China's Chinese herbal medicine resource, and Southwest Henan is located in north-south climate intermediate zone, Fu Niu
Mountain area Chinese herbal medicine resource had both shared similar representativeness, there is the particularity with itself.Funiu Shan Mountain HERBA DENDROBII is commonly called as medicine Huang
Gold, it is cold in nature, it is sweet in flavor, it is light, it is micro- salty.Wild Funiu Shan Mountain HERBA DENDROBII is born on 1000-2000 meters of cliffs of Funiu Shan Mountain height above sea level, stem clump
It is raw.Funiu Shan Mountain HERBA DENDROBII is mainly distributed in the counties such as Xixia, interior township, Nan Zhao, the Lushan of Southwest Henan, has nourishing Yin and moistening lung, benefit
Stomach kidney tonifying, the effects of building brain improving eyesight, be fall fire good medicine.Funiu Shan Mountain HERBA DENDROBII is promoting the production of body fluid to quench thirst, and tonifying five zang organs consumptive disease, clearing lung-heat stops
Cough, Funiu Shan Mountain HERBA DENDROBII has anti-flu, antifatigue etc. to have remarkable efficacy.Funiu Shan Mountain HERBA DENDROBII orchid can significantly mention
High superoxides discrimination China enzyme level reduces lipid peroxide, adjusts brain monoaminergic transmitters level, and similar monoamine is inhibited to aoxidize
Enzyme (MAO), plays the role of anti-aging.Funiu Shan Mountain HERBA DENDROBII orchid contains lactone component, has activating microcirculation and removing stasis medicinal, expansion blood vessel
And the effects of antiplatelet condenses, and treatment embolic occlusion pulse tube is scorching, cerebral thrombosis, arteriosclerotic obliteration.Funiu Shan Mountain gold
The luxuriant and rich with fragrance class and bibenzyl substance extracted in hairpin dendrobium, there is anti-lung cancer, adenocarcinoma ovaries and promyelocitic leukemia.Fu Niu
Mountain HERBA DENDROBII can promote T cell growth and lymphocyte to generate inhibiting factor, play the immune effect of enhancing.Funiu Shan Mountain Jin Chai
The ethyl acetate extract of dendrobium nobile has Human Lung Cancer cell, human body ovary adenocarcinoma cells, human body progranulocyte leukemia
Significant cytotoxic effect.Polysaccharide is the primary pharmacological activity ingredient of dendrobium nobile, but grinding for Funiu Shan Mountain Dendrobium nobile polysaccharide
Study carefully, starting relatively late, controls, content especially for the quality of polysaccharide, in terms of structure and pharmacological activity.With polysaccharide medicine
The research and application of object medically, polysaccharide medicine are gradually developed and utilized.Polysaccharide is the main component of HERBA DENDROBII, is contained
Measure it is relatively high, but the content of polysaccharide influenced by environment and territory it is bigger.Funiu Shan Mountain Dendrobium nobile polysaccharide had in recent years
The significant multiple efficacies such as immune-enhancing activity and anti-aging, anti-spoke body are concerned.
Currently, the preparation method to Funiu Shan Mountain Dendrobium nobile polysaccharide uses following steps:
A. Funiu Shan Mountain HERBA DENDROBII powder 500g is weighed, distilled water 10L is added according to liquid-to-solid ratio 1:20, stirs and evenly mixs；
B. stirring liquid is randomly divided into 10 equal portions, every part 250W sound wave assisted extraction 3 times, No. 3 extracting solutions merging are settled to capacity
In bottle;
C. it is placed on ultrasonic extraction liquid in volumetric flask to shake up, the accurate extracting solution that measures is added into centrifuge tube according to 1:5 ratio
95% ethyl alcohol, shakes up；
D. 4 DEG C of refrigeration 18h, 5000 r/min are centrifuged 30 min after taking-up, abandon supernatant and stay precipitating；
E. it precipitates and 80% ethyl alcohol is added according to 1:5 ratio, wash 2 times；
F. after placing 4h, 5000rpm is centrifuged 20min, collects precipitating, obtains Funiu Shan Mountain HERBA DENDROBII Thick many candies, and freeze-drying is protected
It deposits.
Summary of the invention
The present invention provides a kind of convenient for operation, simple process, extraction polysaccharide to solve shortcoming in the prior art
The extraction of Funiu Shan Mountain Dendrobium nobile polysaccharide with high purity and high content and the method for preparing solid beverage.
In order to solve the above technical problems, the present invention adopts the following technical scheme: the extraction side of Funiu Shan Mountain Dendrobium nobile polysaccharide
Method includes the following steps,
(1) preparation of Funiu Shan Mountain HERBA DENDROBII Thick many candies；
(2) albumen removal is handled；
(3) decolorization；
(4) small molecule removal is handled；
(5) the Thick many candies solution for having removed small molecule is added into dehydrated alcohol and adjusts concentration of alcohol 70%, 4 DEG C stand overnight,
It is centrifuged 15 min under 8000 r/min, collects precipitating, Funiu Shan Mountain Dendrobium nobile polysaccharide after being optimized.
Step (1) is specially including following process:
A. Funiu Shan Mountain HERBA DENDROBII powder 500g is weighed, is that distilled water 10L is added in 1:20 according to feed liquid weight ratio, stirs and evenly mixs；
B. will stirring liquid be randomly divided into 10 equal portions, using 250W sound wave to every part stirring liquid assisted extraction 3 times, by ultrasonic extraction
Liquid merging is settled in volumetric flask；
C. the ultrasonic extraction liquid being placed in volumetric flask shakes up, and the accurate ultrasonic extraction liquid that measures is into centrifuge tube, according to volume ratio
95% ethyl alcohol is added for 1:5 ratio, then shakes up；
D. centrifuge tube is placed on after refrigerating 18h under 4 DEG C of environment, taking out with revolving speed is that 5000 r/min are centrifuged 30 min, is abandoned
Supernatant stays precipitating；
E. precipitating according to weight ratio is that 80% ethyl alcohol is added in 1:5 ratio, is washed 2 times；
F. after placing 4h, it is centrifuged 20min under the revolving speed of 5000rpm, collects precipitating to get slightly more to Funiu Shan Mountain HERBA DENDROBII
Sugar, freeze-drying save.
Step (2) includes following process,
Distilled water dissolution Funiu Shan Mountain HERBA DENDROBII Thick many candies are first added in I, and papain is added by weight for the ratio of 1:3,
Then with 37 DEG C of 5 h of water enzyme digestion, then with 90 DEG C of water-bath enzyme deactivations, enzymatic hydrolysis and enzyme deactivation process are repeated 2 times；
Scavenger reagent is added after II, enzymatic hydrolysis and enzyme deactivation and mixes slowly 2 h, in 15 DEG C of 10 min of standing, is with revolving speed with centrifuge
10000 r/min are centrifuged 15 min, take upper layer faint yellow supernatant, complete the albumen removal of Funiu Shan Mountain HERBA DENDROBII Thick many candies
Processing.
Scavenger reagent uses the mixed solution of chloroform and n-butanol, and the volume ratio of chloroform and n-butanol is 4:1.
Step (3) is specially including following process: first adjusting pH to 8.0 to Deproteinated faint yellow supernatant, then
20%H is added under stiring2O2, so that the concentration of polysaccharide solution is reached 8%, and control temperature at 40 DEG C, stirred in magnetic stirring apparatus
24 h complete the decolorization of Funiu Shan Mountain Dendrobium nobile polysaccharide.
Step (4) is specially including following process: bag filter being cleaned up, polysaccharide solution after decoloration is packed into, then will
Bag filter is placed on magnetic stirring apparatus 24 h that dialyse, and completes the small molecule removal processing in Funiu Shan Mountain Dendrobium nobile polysaccharide.
The method that Funiu Shan Mountain Dendrobium nobile polysaccharide prepares solid beverage takes a certain amount of Funiu Shan Mountain Dendrobium nobile polysaccharide, adds
Adding citric acid and xylitol obtain Funiu Shan Mountain Dendrobium nobile polysaccharide solid beverage after mixing thoroughly.
The method that Funiu Shan Mountain Dendrobium nobile polysaccharide prepares solid beverage takes a certain amount of Funiu Shan Mountain Dendrobium nobile polysaccharide, adds
Add white granulated sugar, maltodextrin and cyclodextrine, Funiu Shan Mountain Dendrobium nobile polysaccharide solid beverage is obtained after mixing thoroughly.
By adopting the above technical scheme, the present invention has operating method simple, is convenient for industrialized mass production, improves Funiu Shan Mountain
The extraction content and purity of Dendrobium nobile polysaccharide, product quality greatly improve, and prepare solid beverage with the polysaccharide, help to improve
The economic value of Funiu Shan Mountain HERBA DENDROBII.
Specific embodiment
The extracting method of Funiu Shan Mountain Dendrobium nobile polysaccharide, includes the following steps,
(1) preparation of Funiu Shan Mountain HERBA DENDROBII Thick many candies；
(2) albumen removal is handled；
(3) decolorization；
(4) small molecule removal is handled；
(5) the Thick many candies solution for having removed small molecule is added into dehydrated alcohol and adjusts concentration of alcohol 70%, 4 DEG C stand overnight,
It is centrifuged 15 min under 8000 r/min, collects precipitating, Funiu Shan Mountain Dendrobium nobile polysaccharide after being optimized.
Step (1) is specially including following process:
A., the offer of Funiu Shan Mountain HERBA DENDROBII powder 500g(Henan Lian Yuan Biotechnology Co., Ltd is provided), according to feed liquid weight
Than distilled water 10L is added for 1:20, stir and evenly mix；
B. will stirring liquid be randomly divided into 10 equal portions, using 250W sound wave to every part stirring liquid assisted extraction 3 times, by ultrasonic extraction
Liquid merging is settled in volumetric flask；
C. the ultrasonic extraction liquid being placed in volumetric flask shakes up, and the accurate ultrasonic extraction liquid that measures is into centrifuge tube, according to volume ratio
95% ethyl alcohol is added for 1:5 ratio, then shakes up；
D. centrifuge tube is placed on after refrigerating 18h under 4 DEG C of environment, taking out with revolving speed is that 5000 r/min are centrifuged 30 min, is abandoned
Supernatant stays precipitating；
E. precipitating according to weight ratio is that 80% ethyl alcohol is added in 1:5 ratio, is washed 2 times；
F. after placing 4h, it is centrifuged 20min under the revolving speed of 5000rpm, collects precipitating to get slightly more to Funiu Shan Mountain HERBA DENDROBII
Sugar, freeze-drying save.
Step (2) includes following process,
Distilled water dissolution Funiu Shan Mountain HERBA DENDROBII Thick many candies are first added in I, and papain is added by weight for the ratio of 1:3,
Then with 37 DEG C of 5 h of water enzyme digestion, then with 90 DEG C of water-bath enzyme deactivations, enzymatic hydrolysis and enzyme deactivation process are repeated 2 times；
Scavenger reagent is added after II, enzymatic hydrolysis and enzyme deactivation and mixes slowly 2 h, in 15 DEG C of 10 min of standing, is with revolving speed with centrifuge
10000 r/min are centrifuged 15 min, take upper layer faint yellow supernatant, complete the albumen removal of Funiu Shan Mountain HERBA DENDROBII Thick many candies
Processing.
Scavenger reagent uses the mixed solution of chloroform and n-butanol, and the volume ratio of chloroform and n-butanol is 4:1.
Step (3) is specially including following process: first adjusting pH to 8.0 to Deproteinated faint yellow supernatant, then
20%H is added under stiring2O2, so that the concentration of polysaccharide solution is reached 8%, and control temperature at 40 DEG C, stirred in magnetic stirring apparatus
24 h complete the decolorization of Funiu Shan Mountain Dendrobium nobile polysaccharide.
Step (4) is specially including following process: bag filter being cleaned up, polysaccharide solution after decoloration is packed into, then will
Bag filter is placed on magnetic stirring apparatus 24 h that dialyse, and completes the small molecule removal processing in Funiu Shan Mountain Dendrobium nobile polysaccharide.
Using the polysaccharide of distinct methods preparation in the present embodiment and background technique, polysaccharide generates comparision contents, in contrast
The present embodiment effect is more preferable, specifically see the table below.
By taking the polysaccharide of the extraction of the present embodiment as an example, the polysaccharide that the present invention is extracted from Funiu Shan Mountain HERBA DENDROBII prepares solid
The method of beverage, including following two embodiment:
Embodiment one: Funiu Shan Mountain Dendrobium nobile polysaccharide additive amount is respectively 0.2,0.4,0.6,0.8,1.0 g, five seed type,
Addition citric acid additive amount is 0.2 g, supplies five kinds of Funiu Shan Mountain Dendrobium nobile polysaccharide solid beverage total weights after xylitol addition
For 3g.
Embodiment two: Funiu Shan Mountain Dendrobium nobile polysaccharide additive amount is respectively 0.2,0.4,0.6,0.8,1.0 g, addition
It is total to supply five kinds of Funiu Shan Mountain Dendrobium nobile polysaccharide solid beverages after cyclodextrine addition by white granulated sugar 0.2g, maltodextrin 0.2g
Weight is 3g.
The sensory evaluation scores of Funiu Shan Mountain Dendrobium nobile polysaccharide solid beverage are made in distinct methods, in contrast embodiment one
Effect is more preferable, specifically see the table below.
The present embodiment not makes any form of restriction shape of the invention, material, structure etc., all according to this hair
Bright technical spirit any simple modification, equivalent change and modification to the above embodiments, belong to the technology of the present invention side
The protection scope of case.
Claims (8)
1. the extracting method of Funiu Shan Mountain Dendrobium nobile polysaccharide, it is characterised in that: include the following steps,
(1) preparation of Funiu Shan Mountain HERBA DENDROBII Thick many candies；
(2) albumen removal is handled；
(3) decolorization；
(4) small molecule removal is handled；
(5) the Thick many candies solution for having removed small molecule is added into dehydrated alcohol and adjusts concentration of alcohol 70%, 4 DEG C stand overnight,
It is centrifuged 15 min under the revolving speed of 8000 r/min, collects precipitating, Funiu Shan Mountain Dendrobium nobile polysaccharide after being optimized.
2. the extracting method of Funiu Shan Mountain Dendrobium nobile polysaccharide according to claim 1, it is characterised in that: step (1) is specific
Being includes following process:
A. Funiu Shan Mountain HERBA DENDROBII powder 500g is weighed, is that distilled water 10L is added in 1:20 according to feed liquid weight ratio, stirs and evenly mixs；
B. will stirring liquid be randomly divided into 10 equal portions, using 250W sound wave to every part stirring liquid assisted extraction 3 times, by ultrasonic extraction
Liquid merging is settled in volumetric flask；
C. the ultrasonic extraction liquid being placed in volumetric flask shakes up, and the accurate ultrasonic extraction liquid that measures is into centrifuge tube, according to volume ratio
95% ethyl alcohol is added for 1:5 ratio, then shakes up；
D. centrifuge tube is placed on after refrigerating 18h under 4 DEG C of environment, taking out with revolving speed is that 5000 r/min are centrifuged 30 min, is abandoned
Supernatant stays precipitating；
E. precipitating according to weight ratio is that 80% ethyl alcohol is added in 1:5 ratio, is washed 2 times；
F. after placing 4h, it is centrifuged 20min under the revolving speed of 5000rpm, collects precipitating to get slightly more to Funiu Shan Mountain HERBA DENDROBII
Sugar, freeze-drying save.
3. the extracting method of Funiu Shan Mountain Dendrobium nobile polysaccharide according to claim 1 or 2, it is characterised in that: step (2) packet
Following process is included,
Distilled water dissolution Funiu Shan Mountain HERBA DENDROBII Thick many candies are first added in I, and papain is added by weight for the ratio of 1:3,
Then with 37 DEG C of 5 h of water enzyme digestion, then with 90 DEG C of water-bath enzyme deactivations, enzymatic hydrolysis and enzyme deactivation process are repeated 2 times；
Scavenger reagent is added after II, enzymatic hydrolysis and enzyme deactivation and mixes slowly 2 h, in 15 DEG C of 10 min of standing, is with revolving speed with centrifuge
10000 r/min are centrifuged 15 min, take upper layer faint yellow supernatant, complete the albumen removal of Funiu Shan Mountain HERBA DENDROBII Thick many candies
Processing.
4. the extracting method of Funiu Shan Mountain Dendrobium nobile polysaccharide according to claim 3, it is characterised in that: scavenger reagent uses
The volume ratio of the mixed solution of chloroform and n-butanol, chloroform and n-butanol is 4:1.
5. the extracting method of Funiu Shan Mountain Dendrobium nobile polysaccharide according to claim 3, it is characterised in that: step (3) is specific
Being includes following process: first adjusting pH to 8.0 to Deproteinated faint yellow supernatant, 20%H is then added under stiring2O2,
So that the concentration of polysaccharide solution is reached 8%, and control temperature at 40 DEG C, 24 h are stirred in magnetic stirring apparatus, completes Funiu Shan Mountain gold
The decolorization of hairpin dendrobium polysaccharide.
6. the extracting method of Funiu Shan Mountain Dendrobium nobile polysaccharide according to claim 5, it is characterised in that: step (4) is specific
Being includes following process: bag filter being cleaned up, polysaccharide solution after decoloration is packed into, bag filter is then placed in magnetic stirring apparatus
24 h of upper dialysis complete the small molecule removal processing in Funiu Shan Mountain Dendrobium nobile polysaccharide.
7. the method for preparing solid beverage using the Funiu Shan Mountain Dendrobium nobile polysaccharide that claim 1 is extracted, it is characterised in that: take one
Quantitative Funiu Shan Mountain Dendrobium nobile polysaccharide adds citric acid and xylitol, Funiu Shan Mountain Dendrobium nobile polysaccharide solid is obtained after mixing thoroughly
Beverage.
8. the method for preparing solid beverage using the Funiu Shan Mountain Dendrobium nobile polysaccharide that claim 1 is extracted, it is characterised in that: take one
Quantitative Funiu Shan Mountain Dendrobium nobile polysaccharide, addition white granulated sugar, maltodextrin and cyclodextrine, obtains Funiu Shan Mountain gold hairpin stone after mixing thoroughly
Dry measure used in former times polysaccharide solid beverage.
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| CN111690076A (en) * | 2020-06-22 | 2020-09-22 | 浙江亚林生物科技股份有限公司 | Dendrobium pectin extraction process method |
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Application publication date: 20191018 |