CN102204946B - Chinese lobelia polysaccharide extracted from Chinese lobelia and application thereof - Google Patents
Chinese lobelia polysaccharide extracted from Chinese lobelia and application thereof Download PDFInfo
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Abstract
The invention relates to Chinese lobelia polysaccharide extracted from Chinese lobelia and application thereof. The solved technical scheme comprises the following steps: adding water in the taken Chinese lobelia medicinal material to carry out water bath for 2-3 times; filtering, mixing filtrate, and concentrating until the weight volume of the concentrated liquid is 1-2 times that of a medicinal material; adding above 90% ethanol by weight until the alcohol content reaches 80%; standing for 12-24 hours, and centrifuging so as to obtain a polysaccharide precipitate; successively washing the precipitate with absolute ethanol, acetone and diethyl ether which are used as washing liquids, wherein for every 1-5g of precipitate, the used amounts of absolute ethanol, acetone and diethyl ether are 80-150 ml; and drying the washed precipitate so as to obtain the rice white and powdery Chinese lobelia polysaccharide. The Chinese lobelia polysaccharide is effectively used for preparing medicaments capable of enhancing human immunologic function, thereby achieving application of the Chinese lobelia in the aspect of preparing the medicaments capable of enhancing the human immunologic function and creating a novel application prospect of Chinese lobelia medicinal material. The Chinese lobelia extract can also be used for preparing health care products, thereby achieving the application of the Chinese lobelia in health care preparation. The Chinese lobelia polysaccharide has huge economic and social benefits.
Description
Technical field
The present invention relates to field of medicaments, particularly a kind of Chinese lobelia polysaccharide that from Herba Lobeliae Chinensis, extracts and application thereof.
Background technology
Along with to the going deep into of Chinese medicine comprehensive study, the active component in the crude drug in whole is extracted respectively, is developed and by becoming effective, is that life is injected in the development of Natural Medicine Chemistry, and wherein the polysaccharide composition is exactly an extremely important part.In recent years, the various countries scholar is very fast to the research and development of polysaccharide, only the mushroom polysaccharide is inquired into the research that develops into the many sections of higher plant genus, many tissues, many organs by initial.Wherein with its promotion with to recover the research of immunologic function of body particularly outstanding.Studies show that, polysaccharide is as a kind of immunological enhancement and regulator, it is the main immunologic active material of many natural drugs, generally have advantages of antibiotic, antiviral, parasiticide, antitumor, radioprotective, defying age and normal cell is had no side effect, thus of common occurrence based on the report of polysaccharide composition activity in the various medicinal plants.
Herba Lobeliae Chinensis begins to be stated from " the southern regions of the Yunnan Province book on Chinese herbal medicine ", and rear Li Shizhen (1518-1593 A.D.) is also on the books in Compendium of Material Medica
[1], have heat-clearing and toxic substances removing, the effect of inducing diuresis to remove edema.Clinical treatment face foot edema, carbuncle furuncle, snake bite and insect sting, the advanced schistosomiasis ascites of being mainly used in
[2]Deng disease.At present, still stop at its contained alkaloid, flavonoid glycoside, Saponin and aminoacid ingredient for the research of Chinese medicine Herba Lobeliae Chinensis, the extraction of the polysaccharide composition of its rich content is separated and strengthens immune function of human body having no open report.
Summary of the invention
Based on above-mentioned situation, for further developing medicinal, the economic worth of Herba Lobeliae Chinensis medical material, the research of filling up this respect is blank, and for overcoming the defective of prior art, purpose of the present invention just provides a kind of Chinese lobelia polysaccharide that extracts and application thereof from Herba Lobeliae Chinensis.
The technical scheme that the present invention solves is: get the Herba Lobeliae Chinensis medical material, press the medical material weighing scale, add medical material weight 20-40 water doubly, 70～90 ℃ of water-baths are extracted 2～3 times, each 1～3h, filter, merging filtrate, be concentrated into the 1-2 of medical material bulking value doubly, get the polysaccharide concentrated solution, bulking value refers to that the solid medical material is in g, (bulking value is expressed as liquid water: W/V) in ml, the ethanol that adds mass concentration again and be more than 90% reaches 80% to containing the alcohol amount, leaves standstill 12～24h, centrifugal polysaccharide precipitation thing, this precipitate is used dehydrated alcohol successively, acetone, ether washs as cleaning mixture, every 1-5g precipitate, dehydrated alcohol, acetone, the ether consumption is 80-150ml, and the precipitate after the washing is carried out drying, get rice white, pulverous Chinese lobelia polysaccharide, be effective to prepare the medicine that strengthens immune function of human body, realize the application of Herba Lobeliae Chinensis in the medicine of preparation enhancing immune function of human body, the new prospect of application of opening up the Herba Lobeliae Chinensis medical material.Herba Lobeliae Chinensis extract of the present invention also can be used for preparing health product, realizes the application of Herba Lobeliae Chinensis in the preparation health product, and huge social and economic benefits is arranged.
The specific embodiment
Below in conjunction with concrete condition and embodiment the specific embodiment of the present invention is elaborated.
Provide by the foregoing invention content, the Herba Lobeliae Chinensis pulverizing medicinal materials is become the Herba Lobeliae Chinensis medicinal powder, get the Herba Lobeliae Chinensis medicinal powder, press medicinal powder and water, the ratio of feed liquid w/v 1:20-40 adds entry in medicinal powder, stir evenly; 70～90 ℃ of water-baths are extracted 1～3 time, and each 1～3h filters; Merging filtrate, concentrated filtrate gets the polysaccharide concentrated solution to 1-2 times of Herba Lobeliae Chinensis medicine mast bulking value; Slow adding mass concentration is the ethanol more than 90% and fully stirs in the polysaccharide concentrated solution, reaches 80% until contain pure measuring, sealing, and 4 ℃ of cold preservations are left standstill; The centrifugal polysaccharide precipitation thing that gets behind 12～24h, described precipitate is successively with dehydrated alcohol, acetone, ether washing, washing 1～5g precipitate dehydrated alcohol, acetone, ether consumption are 80ml～150ml, to the centrifuged supernatant clear, reach remove portion impurity, precipitate after the washing is carried out drying, get rice white, Powdered Chinese lobelia polysaccharide, polysaccharide yield is 20.28%～22.78%, measuring sugared content through phenolsulfuric acid method (conventional determining method is known technology) is 72.78%～77.41%.
Described Herba Lobeliae Chinensis medicinal powder refers to pulverize the powder of No. 2 pharmacopeia sieves of rear mistake.
The invention will be further described below by embodiment.
Embodiment 1
The Herba Lobeliae Chinensis pulverizing medicinal materials is become the Herba Lobeliae Chinensis medicinal powder, get the Herba Lobeliae Chinensis medicinal powder, the ratio in medicinal powder and water feed liquid w/v 1:20 adds entry in medicinal powder, stir evenly; 70 ℃ of water-baths are extracted 3 times, and each 1h filters; Merging filtrate, concentrated filtrate gets the polysaccharide concentrated solution to 1 times of Herba Lobeliae Chinensis medicine mast bulking value; Slowly adding mass concentration in the polysaccharide concentrated solution is 90% ethanol and fully stirring, reaches 80% until contain the alcohol amount, sealing, and 4 ℃ of cold preservations are left standstill; The centrifugal polysaccharide precipitation thing that gets behind the 12h, described precipitate is successively with dehydrated alcohol, acetone, ether washing, washing 1g precipitate dehydrated alcohol, acetone, ether consumption are 80ml, to the centrifuged supernatant clear, reach remove portion impurity, the precipitate after the washing is carried out drying, get rice white, Powdered Chinese lobelia polysaccharide, polysaccharide yield is 21.38%, and measuring sugared content through the phenolsulfuric acid method is 75.23%.
Embodiment 2
The Herba Lobeliae Chinensis pulverizing medicinal materials is become the Herba Lobeliae Chinensis medicinal powder, get the Herba Lobeliae Chinensis medicinal powder, the ratio in medicinal powder and water feed liquid w/v 1:30 adds entry in medicinal powder, stir evenly; 80 ℃ of water-baths are extracted 2 times, and each 1.5h filters; Merging filtrate, concentrated filtrate gets the polysaccharide concentrated solution to 1.5 times of Herba Lobeliae Chinensis medicine mast bulking value; Slowly adding mass concentration in the polysaccharide concentrated solution is 95% ethanol and fully stirring, reaches 80% until contain the alcohol amount, sealing, and 4 ℃ of cold preservations are left standstill; The centrifugal polysaccharide precipitation thing that gets behind the 24h, described precipitate is successively with dehydrated alcohol, acetone, ether washing, washing 3g precipitate dehydrated alcohol, acetone, ether consumption are 100ml, to the centrifuged supernatant clear, reach remove portion impurity, the precipitate after the washing is carried out drying, get rice white, Powdered Chinese lobelia polysaccharide, polysaccharide yield is 22.01%, and measuring sugared content through the phenolsulfuric acid method is 74.57%.
Embodiment 3
The Herba Lobeliae Chinensis pulverizing medicinal materials is become the Herba Lobeliae Chinensis medicinal powder, get the Herba Lobeliae Chinensis medicinal powder, the ratio in medicinal powder and water feed liquid w/v 1:40 adds entry in medicinal powder, stir evenly; 90 ℃ of water-baths are extracted 3 times, and each 3h filters; Merging filtrate, concentrated filtrate gets the polysaccharide concentrated solution to 2 times of Herba Lobeliae Chinensis medicine mast bulking value; Slowly adding mass concentration in the polysaccharide concentrated solution is 95% ethanol and fully stirring, reaches 80% until contain the alcohol amount, sealing, and 4 ℃ of cold preservations are left standstill; The centrifugal polysaccharide precipitation thing that gets behind the 20h, described precipitate is successively with dehydrated alcohol, acetone, ether washing, washing 5g precipitate dehydrated alcohol, acetone, ether consumption are 150ml, to the centrifuged supernatant clear, reach remove portion impurity, the precipitate after the washing is carried out drying, get rice white, Powdered Chinese lobelia polysaccharide, polysaccharide yield is 22.32%, and measuring sugared content through the phenolsulfuric acid method is 76.26%.
Above-mentioned only is the several embodiment that provide, in development, the inventor repeats 3 times through repeated multiple times at every turn at least, has all obtained identical or akin result, extract is stable, method is reliable, and extract is carried out repeatedly test all obtained satisfied technique effect, shows that fully Chinese lobelia polysaccharide has the effect that strengthens immunity, be the zoopery sufficient proof, the situation of specifically testing is as follows:
One. test material
1. experimental animal: 18～22g Kunming kind cleaning agent mice, male and female half and half.Provided by Hebei province's Experimental Animal Center.
2. experiment reagent: Chinese lobelia polysaccharide, Switzerland's dye liquor, lentinan (Hubei Guangren Pharmaceutical Co., Ltd.), cyclophosphamide (Hengrui Medicine Co., Ltd., Jiangsu Prov.), sodium citrate, calcium chloride, sodium chloride, sodium potassium tartrate tetrahydrate, potassium chloride, glucose, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, phenol red.
Two. test method
1. Chinese lobelia polysaccharide is on the impact of normal Phagocytosis By The Peritoneal Macrophages In Mice
Get 50 of mices, body weight 18～22g, male and female half and half evenly are divided into 5 groups at random.Gavage respectively little, in, heavy dose of Chinese lobelia polysaccharide aqueous solution (10mg/ml, 20mg/ml, 40mg/ml; 0.2ml/10g), lentinan suspension (5mg/ml; 0.2ml/10g) and with the normal saline (0.2ml/10g) of volume.Administration every day 1 time, successive administration 7 days.Respectively organized the equal lumbar injection 5% chicken red blood cell normal saline suspension 0.5ml of mice 7 day morning.2h behind the 7th day gastric infusion, inject 5% chicken red blood cell 4h after, take off cervical vertebra and put to death mice, Intraperitoneal injection Han Shi liquid 2.5ml gently rubs mouse web portion; Then cut off mouse part skin, cut an aperture at peritoneum, draw peritoneal fluid 2ml with suction pipe and place test tube, mixing; Draw a little abdominal cavity drop on microscope slide, liquid point size is about 1.5cm * 2cm.Microscope slide is placed in the sugared porcelain dish that is covered with wet gauze, hatches 30min for 37 ℃, normal saline washes away the cell that adheres to, Wright's stain dyeing, and the tap water flushing is dried; Microscopically is observed the situation of engulfing of Turnover of Mouse Peritoneal Macrophages, and calculates phagocytic percentage and phagocytic index.
Table 1 Peritoneal Macrophage Phagocytosis result (± s)
| Group | Number of animals (only) | Dosage (g/kg) | Phagocytic percentage (%) | Phagocytic index |
| The blank group | 10 | ? | 31.0±4.0 | 0.323±0.005 |
| The lentinan group | 10 | 0.1 | 54.7±7.4** | 0.614±0.004** |
| Low dose of polysaccharide group | 10 | 0.2 | 62.1±5.9** | 0.711±0.007** |
| Middle dosage polysaccharide group | 10 | 0.4 | 58.5±5.4** | 0.692±0.009** |
| Heavy dose of polysaccharide group | 10 | 0.8 | 50.1±4.9* | 0.578±0.010* |
* represents that with the blank group than P＜0.01, * represents with the blank group than P＜0.05
As can be seen from Table 1, with blank group ratio, heavy dose of group can be improved Turnover of Mouse Peritoneal Macrophages to the phagocytic percentage of chicken red blood cell, can improve the phagocytic index (P＜0.05) of peritoneal macrophage; Little, middle dosage group and lentinan group all can significantly improve phagocytic percentage and phagocytic index (P＜0.01), wherein especially act as the strongest with low dose of Chinese lobelia polysaccharide group.
2. Chinese lobelia polysaccharide is on the impact of normal mouse hemolysin formation
Get 50 of mices, body weight 18～22g, male and female half and half evenly are divided into 5 groups at random.Gavage respectively little, in, heavy dose of Chinese lobelia polysaccharide aqueous solution (10mg/ml, 20mg/ml, 40mg/ml; 0.2ml/10g), lentinan suspension (5mg/ml; 0.2ml/10g) and with the normal saline (0.2ml/10g) of volume.Administration every day 1 time, successive administration 7 days.Simultaneously, administration was only respectively organized the equal lumbar injection 5% chicken red blood cell normal saline suspension 0.2ml/ of mice on the 1st day, carried out immunity, 2h after last 1 administration, and mouse orbit is got blood, and is centrifugal, separation of serum.After normal saline 1: 100 dilution, get 1ml diluent and 5% chicken red blood cell suspension 0.5ml, 10% complement 0.5ml(guinea pig serum, with chicken red blood cell saturated 6h in advance) mixing, hatch 30min for 37 ℃, cessation reaction in the frozen water.Other establishes the blank tube that does not add complement and compares, and draws and respectively manages supernatant in UV-2201 type spectrophotometer 540nm place colorimetric, measures and respectively organizes the hemolysin formational situation.
3. Chinese lobelia polysaccharide is on the impact of normal mouse hemolysis plaque formation
Get 50 of mices, body weight 18～22g, male and female half and half evenly are divided into 5 groups at random.Gavage respectively little, in, heavy dose of Chinese lobelia polysaccharide aqueous solution (10mg/ml, 20mg/ml, 40mg/ml; 0.2ml/10g), lentinan suspension (5mg/ml; 0.2ml/10g) and with the normal saline (0.2ml/10g) of volume.Administration every day 1 time, successive administration 7 days.Simultaneously, administration was only respectively organized the equal lumbar injection 5% chicken red blood cell normal saline suspension 0.2ml/ of mice on the 1st day, carried out immunity, and 2h after last 1 administration takes off cervical vertebra and puts to death and dissect mice, takes out spleen, and two mouse spleens are an example on the same group; Homogenate, and spleens cell number is 5 * 10 in the adjustment splenocyte suspension
6Individual/ml.Extracting spleen cell suspension 1.0ml is with the guinea pig serum 0.5ml mixing of 0.2% chicken red blood cell suspension 0.5ml and 1: 10.Other establishes the blank tube that does not add complement, hatches 1h for 37 ℃, and is centrifugal, gets supernatant in UV-2201 type spectrophotometer 413nm place colorimetric, surveys and respectively organizes the hemolysis plaque formational situation.
Table 2 hemolysin, hemolysis plaque form the result (± s)
| Group | Number of animals (only) | Dosage (g/kg) | Hemolysin forms | The example number | Hemolysis plaque forms |
| The blank group | 10 | ? | 0.5366±0.0004 | 5 | 0.5874±0.0294 |
| The lentinan group | 10 | 0.1 | 0.719±0.003** | 5 | 0.6628±0.0222* |
| Low dose of polysaccharide group | 10 | 0.2 | 0.744±0.0007** | 5 | 0.7093±0.02008** |
| Middle dosage polysaccharide group | 10 | 0.4 | 0.731±0.001* | 5 | 0.6873±0.01794* |
| Heavy dose of polysaccharide group | 10 | 0.8 | 0.705±0.0006* | 5 | 0.6537±0.02308* |
* represents that with the blank group than P＜0.01, * represents with the blank group than P＜0.05
As can be seen from Table 2, with blank group ratio, in, heavy dose of group hemolysis plaque all raises (P＜0.05), small dose group and lentinan group hemolysin and hemolysis plaque be significantly increased (P＜0.01).Wherein especially act as optimum with low dose of Chinese lobelia polysaccharide group.
4. the impact of Chinese lobelia polysaccharide immunosuppressed mice Peritoneal Macrophage Phagocytosis that cyclophosphamide is induced
Get 60 of mices, body weight 18～22g, male and female half and half evenly are divided into 6 groups at random.Except the blank group, all the other each group is all set up cyclophosphamide and is caused totally 5 groups on immunosuppressant model (dosage is 8 mg/ml; 1st, 2,3 days continuous lumbar injections), model group gavages respectively Chinese lobelia polysaccharide aqueous solution (5mg/ml, 10mg/ml, 20mg/ml after setting up and finishing; 0.2ml/10g), lentinan suspension (5mg/ml, 0.2ml/10g) reaches the normal saline (0.2ml/10g) with volume.Administration every day 1 time, successive administration 7 days.Respectively organize the equal lumbar injection 5% chicken red blood cell normal saline suspension 0.5ml of mice morning last day in administration, 2h behind the gastric infusion to 4h behind the chicken red blood cell, takes off cervical vertebra and puts to death mice.Intraperitoneal injection Han Shi liquid 2.5ml gently rubs mouse web portion, then cuts off mouse part skin, cuts an aperture at peritoneum, draws peritoneal fluid 2ml with liquid-transfering gun and places test tube, mixing; Draw a little abdominal cavity drop on microscope slide, liquid point size is about 1.5cm * 2cm.Microscope slide is placed in the sugared porcelain dish that is covered with wet gauze, hatches 30min for 37 ℃, normal saline washes away the cell that adheres to, Wright's stain dyeing, the tap water flushing is dried, and microscopically is observed the situation of engulfing of Turnover of Mouse Peritoneal Macrophages, and calculates phagocytic percentage and phagocytic index.
Table 3 Peritoneal Macrophage Phagocytosis result (± s)
| Group | Number of animals (only) | Dosage (g/kg) | Phagocytic percentage (%) | Phagocytic index |
| The blank group | 10 | ? | 50.3±2.0 | 0.523±0.007 |
| Model group | 10 | ? | 31.7±9.3 | 0.323±0.002 |
| The lentinan group | 10 | 0.1 | 56.3±7.4** | 0.571±0.011** |
| 5mg/ml Chinese lobelia polysaccharide group | 10 | 0.1 | 61.5±3.2** | 0.632±0.009** |
| 10mg/ml Chinese lobelia polysaccharide group | 10 | 0.2 | 59.1±2.8** | 0.618±0.004** |
| 20mg/ml Chinese lobelia polysaccharide group | 10 | 0.4 | 57.2±4.1** | 0.601±0.005** |
* represents with model group than P＜0.01
As can be seen from Table 3, compare with the blank group, the model group Turnover of Mouse Peritoneal Macrophages significantly reduces (P＜0.01) to phagocytic index and the phagocytic percentage of chicken red blood cell, and the modeling success is described.Compare with model group, heavy dose of polysaccharide group and lentinan group can significantly improve Turnover of Mouse Peritoneal Macrophages to phagocytic index and the phagocytic percentage (P＜0.01) of chicken red blood cell.
5. the impact that forms of Chinese lobelia polysaccharide immunosuppressed mice hemolysin that cyclophosphamide is induced
Get 60 of mices, body weight 18～22g, male and female half and half evenly are divided into 6 groups at random.Except the blank group, all the other each group is all set up cyclophosphamide and is caused totally 5 groups on immunosuppressant model (dosage is 8 mg/ml; 1st, 2,3 days continuous lumbar injections); After model group was set up and finished, each organized all lumbar injection 5% chicken red blood cell normal saline suspension 0.2ml/ of mice (containing blank group), carries out immunity; And begin to gavage respectively Chinese lobelia polysaccharide aqueous solution (5mg/ml, 10mg/ml, 20mg/ml; 0.2ml/10g), lentinan suspension (5mg/ml, 0.2ml/10g) and with the normal saline (0.2ml/10g) of volume, administration every day 1 time, successive administration 7 days.2h after last 1 administration, mouse orbit is got blood, and is centrifugal, separation of serum; After normal saline 1: 100 dilution, get 1ml diluent and 5% chicken red blood cell suspension 0.5ml, 10% complement 0.5ml(guinea pig serum, with chicken red blood cell saturated 6h in advance) mixing; Hatch 30min for 37 ℃, cessation reaction in the frozen water, other establishes the blank tube that does not add complement and compares; Draw and respectively manage supernatant in UV-2201 type spectrophotometer 540nm place colorimetric, measure and respectively organize the hemolysin formational situation.
6. the impact that forms of Chinese lobelia polysaccharide immunosuppressed mice hemolysis plaque that cyclophosphamide is induced
Get 60 of mices, body weight 18～22g, male and female half and half evenly are divided into 6 groups at random.Except the blank group, all the other each group is all set up cyclophosphamide and is caused totally 5 groups on immunosuppressant model (dosage is 8 mg/ml; 1st, 2,3 days continuous lumbar injections); After model group was set up and finished, each organized all lumbar injection 5% chicken red blood cell normal saline suspension 0.2ml/ of mice (containing blank group), carries out immunity; And begin to gavage respectively Chinese lobelia polysaccharide aqueous solution (5mg/ml, 10mg/ml, 20mg/ml; 0.2ml/10g), lentinan suspension (5mg/ml, 0.2ml/10g) and with the normal saline (0.2ml/10g) of volume, administration every day 1 time, successive administration 7 days.2h after last 1 administration takes off that cervical vertebra is put to death and dissect mice, takes out spleen, on the same group in two mouse spleens be an example, homogenate, and adjust that spleens cell number is 5 * 10 in the splenocyte suspension
6Individual/ml.Extracting spleen cell suspension 1.0ml is with the guinea pig serum 0.5ml mixing of 0.2% chicken red blood cell suspension 0.5ml and 1: 10.Other establishes the blank tube that does not add complement, hatches 1h for 37 ℃, and is centrifugal, gets supernatant in UV-2201 type spectrophotometer 413nm place colorimetric, surveys and respectively organizes the hemolysis plaque formational situation.
Table 4 hemolysin, hemolysis plaque form the result (± s)
| Group | Number of animals (only) | Dosage (g/kg) | Hemolysin forms | The example number | Hemolysis plaque forms |
| The blank group | 10 | ? | 0.552±0.013 | 5 | 0.573±0.021 |
| Model group | 10 | ? | 0.401±0.0087 | 5 | 0.408±0.019 |
| The lentinan group | 10 | 0.1 | 0.656±0.0064** | 5 | 0.639±0.014** |
| 5mg/ml Herba Lobeliae Chinensis group | 10 | 0.1 | 0.727±0.0037** | 5 | 0.7041±0.0103** |
| 10mg/ml Herba Lobeliae Chinensis group | 10 | 0.2 | 0.680±0.0018** | 5 | 0.6404±0.0115** |
| 20mg/ml Herba Lobeliae Chinensis group | 10 | 0.4 | 0.653±0.0036** | 5 | 0.6241±0.0213** |
* represents with model group than P＜0.01
Can find out from upper table, with blank group ratio, model group hemolysin and hemolysis plaque all significantly reduce (P＜0.01), illustrate and make the success of immunosuppressant model.With the model group ratio, each dosage Chinese lobelia polysaccharide group and lentinan group all can significantly promote the formation (P＜0.01) of mice hemolysin and hemolysis plaque.Wherein, especially take 0.1 g/kg dosage Chinese lobelia polysaccharide group as best.
Verify through zoopery, the Chinese lobelia polysaccharide constituents that extracts among the present invention has the effect of good enhancing human body immunity power, possess the potentiality and the actual promotional value that are developed as health-related food and immunostimulant fully, this constituents can be used for the symptom of the hypoimmunity that caused by many reasons such as hypotrophy, unordered, overtired, the tumour patient chemicotherapies of life.The developmental research Chinese lobelia polysaccharide not only can be widened the new prospect that the Chinese medicine Herba Lobeliae Chinensis is used, and follows especially era step closely to the creative contribution of Chinese medicine, has huge economy and social benefit.
Claims (2)
1. the application of the Chinese lobelia polysaccharide that from Herba Lobeliae Chinensis, extracts in preparation enhancing immunologic function medicine.
2. the application of the Chinese lobelia polysaccharide that from Herba Lobeliae Chinensis, extracts in preparation enhancing immunologic function health product.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1961898A (en) * | 2005-11-10 | 2007-05-16 | 黄振华 | An antitumor compound pharmaceutical composition with barbed stullcap and preparation process thereof |
| CN101837065A (en) * | 2010-05-13 | 2010-09-22 | 粤北人民医院 | Medicine with anti-hepatitis, anti-tumour and organism immunity improving functions and preparation method thereof |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1961898A (en) * | 2005-11-10 | 2007-05-16 | 黄振华 | An antitumor compound pharmaceutical composition with barbed stullcap and preparation process thereof |
| CN101837065A (en) * | 2010-05-13 | 2010-09-22 | 粤北人民医院 | Medicine with anti-hepatitis, anti-tumour and organism immunity improving functions and preparation method thereof |
Non-Patent Citations (5)
| Title |
|---|
| 刘法锦等.半边莲中氨基酸成分的研究.《湖南中医杂志》.1992,(第4期),48. |
| 半边莲中氨基酸成分的研究;刘法锦等;《湖南中医杂志》;19921231(第4期);48 * |
| 半边莲的研究进展;赵勇等;《医学信息》;20060531;第19卷(第5期);1115-1116 * |
| 王红艳等.复方半边莲注射液治疗小儿秋季腹泻.《白求恩军医学院学报》.2007,第5卷(第2期),88-89. * |
| 赵勇等.半边莲的研究进展.《医学信息》.2006,第19卷(第5期),1115-1116. |
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