CN107455741A - A kind of health food with anti-oxidation function and its preparation method and application - Google Patents
A kind of health food with anti-oxidation function and its preparation method and application Download PDFInfo
- Publication number
- CN107455741A CN107455741A CN201710672083.2A CN201710672083A CN107455741A CN 107455741 A CN107455741 A CN 107455741A CN 201710672083 A CN201710672083 A CN 201710672083A CN 107455741 A CN107455741 A CN 107455741A
- Authority
- CN
- China
- Prior art keywords
- extract
- polysaccharide
- health food
- golden
- hazelnut
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000013402 health food Nutrition 0.000 title claims abstract description 37
- 230000003064 anti-oxidating effect Effects 0.000 title claims abstract description 35
- 238000002360 preparation method Methods 0.000 title claims abstract description 35
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 128
- 239000000284 extract Substances 0.000 claims abstract description 87
- 150000004676 glycans Chemical class 0.000 claims abstract description 52
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 52
- 239000005017 polysaccharide Substances 0.000 claims abstract description 52
- 244000302512 Momordica charantia Species 0.000 claims abstract description 44
- 235000009811 Momordica charantia Nutrition 0.000 claims abstract description 44
- 235000009812 Momordica cochinchinensis Nutrition 0.000 claims abstract description 44
- 235000018365 Momordica dioica Nutrition 0.000 claims abstract description 44
- 235000007466 Corylus avellana Nutrition 0.000 claims abstract description 42
- 235000001543 Corylus americana Nutrition 0.000 claims abstract description 39
- 235000002594 Solanum nigrum Nutrition 0.000 claims abstract description 32
- 240000002307 Solanum ptychanthum Species 0.000 claims abstract description 32
- 244000171263 Ribes grossularia Species 0.000 claims abstract description 29
- 235000002357 Ribes grossularia Nutrition 0.000 claims abstract description 29
- 235000013305 food Nutrition 0.000 claims abstract description 25
- 238000000034 method Methods 0.000 claims abstract description 8
- 239000011122 softwood Substances 0.000 claims abstract description 7
- 235000020985 whole grains Nutrition 0.000 claims abstract description 4
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 60
- 235000019441 ethanol Nutrition 0.000 claims description 45
- 241000723382 Corylus Species 0.000 claims description 39
- 239000000843 powder Substances 0.000 claims description 26
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 25
- 238000000605 extraction Methods 0.000 claims description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 238000005238 degreasing Methods 0.000 claims description 15
- 238000010438 heat treatment Methods 0.000 claims description 15
- 238000011084 recovery Methods 0.000 claims description 15
- 238000010992 reflux Methods 0.000 claims description 15
- 239000012153 distilled water Substances 0.000 claims description 13
- 150000002632 lipids Chemical class 0.000 claims description 10
- 238000001556 precipitation Methods 0.000 claims description 10
- 239000006228 supernatant Substances 0.000 claims description 10
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 10
- 238000005119 centrifugation Methods 0.000 claims description 9
- 210000004369 blood Anatomy 0.000 claims description 8
- 239000008280 blood Substances 0.000 claims description 8
- 238000001914 filtration Methods 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 7
- 238000007710 freezing Methods 0.000 claims description 6
- 230000008014 freezing Effects 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 239000002245 particle Substances 0.000 claims description 6
- WORJEOGGNQDSOE-UHFFFAOYSA-N chloroform;methanol Chemical compound OC.ClC(Cl)Cl WORJEOGGNQDSOE-UHFFFAOYSA-N 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 5
- 238000013461 design Methods 0.000 claims description 5
- 235000013399 edible fruits Nutrition 0.000 claims description 5
- 239000003480 eluent Substances 0.000 claims description 5
- 238000010828 elution Methods 0.000 claims description 5
- 239000000469 ethanolic extract Substances 0.000 claims description 5
- 235000003599 food sweetener Nutrition 0.000 claims description 5
- 239000003208 petroleum Substances 0.000 claims description 5
- 238000000746 purification Methods 0.000 claims description 5
- 239000002994 raw material Substances 0.000 claims description 5
- 238000004064 recycling Methods 0.000 claims description 5
- 239000011347 resin Substances 0.000 claims description 5
- 229920005989 resin Polymers 0.000 claims description 5
- 239000003381 stabilizer Substances 0.000 claims description 5
- 239000003765 sweetening agent Substances 0.000 claims description 5
- 239000000706 filtrate Substances 0.000 claims description 4
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 claims description 4
- UEDUENGHJMELGK-HYDKPPNVSA-N Stevioside Chemical group O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O UEDUENGHJMELGK-HYDKPPNVSA-N 0.000 claims description 3
- 239000004376 Sucralose Substances 0.000 claims description 3
- 239000003292 glue Substances 0.000 claims description 3
- 229930189775 mogroside Natural products 0.000 claims description 3
- 239000000047 product Substances 0.000 claims description 3
- 239000001334 starch sodium octenyl succinate Substances 0.000 claims description 3
- 235000013826 starch sodium octenyl succinate Nutrition 0.000 claims description 3
- 229940013618 stevioside Drugs 0.000 claims description 3
- OHHNJQXIOPOJSC-UHFFFAOYSA-N stevioside Natural products CC1(CCCC2(C)C3(C)CCC4(CC3(CCC12C)CC4=C)OC5OC(CO)C(O)C(O)C5OC6OC(CO)C(O)C(O)C6O)C(=O)OC7OC(CO)C(O)C(O)C7O OHHNJQXIOPOJSC-UHFFFAOYSA-N 0.000 claims description 3
- 235000019202 steviosides Nutrition 0.000 claims description 3
- BAQAVOSOZGMPRM-QBMZZYIRSA-N sucralose Chemical compound O[C@@H]1[C@@H](O)[C@@H](Cl)[C@@H](CO)O[C@@H]1O[C@@]1(CCl)[C@@H](O)[C@H](O)[C@@H](CCl)O1 BAQAVOSOZGMPRM-QBMZZYIRSA-N 0.000 claims description 3
- 235000019408 sucralose Nutrition 0.000 claims description 3
- 240000007582 Corylus avellana Species 0.000 claims 1
- 239000002893 slag Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 15
- 239000003963 antioxidant agent Substances 0.000 abstract description 12
- 235000006708 antioxidants Nutrition 0.000 abstract description 12
- 230000003078 antioxidant effect Effects 0.000 abstract description 9
- 230000003712 anti-aging effect Effects 0.000 abstract description 3
- 230000000295 complement effect Effects 0.000 abstract description 2
- 229940094952 green tea extract Drugs 0.000 abstract description 2
- 235000020688 green tea extract Nutrition 0.000 abstract description 2
- 241000894007 species Species 0.000 abstract description 2
- 240000009226 Corylus americana Species 0.000 abstract 2
- 239000000243 solution Substances 0.000 description 38
- 239000000203 mixture Substances 0.000 description 34
- 238000012360 testing method Methods 0.000 description 11
- 241000699666 Mus <mouse, genus> Species 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 8
- 241001465754 Metazoa Species 0.000 description 7
- 230000037396 body weight Effects 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 6
- 229920002774 Maltodextrin Polymers 0.000 description 5
- 230000002496 gastric effect Effects 0.000 description 5
- 238000001802 infusion Methods 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 5
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 4
- 102000011759 adducin Human genes 0.000 description 4
- 108010076723 adducin Proteins 0.000 description 4
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 4
- 239000010931 gold Substances 0.000 description 4
- 229910052737 gold Inorganic materials 0.000 description 4
- -1 hydroxyl radical free radical Chemical class 0.000 description 4
- 210000002381 plasma Anatomy 0.000 description 4
- 239000012498 ultrapure water Substances 0.000 description 4
- 108010028554 LDL Cholesterol Proteins 0.000 description 3
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 3
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 229910021642 ultra pure water Inorganic materials 0.000 description 3
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- 108010023302 HDL Cholesterol Proteins 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 238000011888 autopsy Methods 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 210000002216 heart Anatomy 0.000 description 2
- 230000031700 light absorption Effects 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 230000001590 oxidative effect Effects 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- WQGWDDDVZFFDIG-UHFFFAOYSA-N pyrogallol Chemical compound OC1=CC=CC(O)=C1O WQGWDDDVZFFDIG-UHFFFAOYSA-N 0.000 description 2
- 150000003254 radicals Chemical class 0.000 description 2
- 230000002000 scavenging effect Effects 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 235000013616 tea Nutrition 0.000 description 2
- 235000020795 whole food diet Nutrition 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 108010010234 HDL Lipoproteins Proteins 0.000 description 1
- 102000015779 HDL Lipoproteins Human genes 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- 238000012404 In vitro experiment Methods 0.000 description 1
- 239000005913 Maltodextrin Substances 0.000 description 1
- PCZOHLXUXFIOCF-UHFFFAOYSA-N Monacolin X Natural products C12C(OC(=O)C(C)CC)CC(C)C=C2C=CC(C)C1CCC1CC(O)CC(=O)O1 PCZOHLXUXFIOCF-UHFFFAOYSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- YNPNZTXNASCQKK-UHFFFAOYSA-N Phenanthrene Natural products C1=CC=C2C3=CC=CC=C3C=CC2=C1 YNPNZTXNASCQKK-UHFFFAOYSA-N 0.000 description 1
- 241000786103 Steatomys pratensis Species 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- DGEZNRSVGBDHLK-UHFFFAOYSA-N [1,10]phenanthroline Chemical compound C1=CN=C2C3=NC=CC=C3C=CC2=C1 DGEZNRSVGBDHLK-UHFFFAOYSA-N 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 235000021466 carotenoid Nutrition 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 210000002969 egg yolk Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 201000005577 familial hyperlipidemia Diseases 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 230000037308 hair color Effects 0.000 description 1
- 239000008236 heating water Substances 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- PCZOHLXUXFIOCF-BXMDZJJMSA-N lovastatin Chemical compound C([C@H]1[C@@H](C)C=CC2=C[C@H](C)C[C@@H]([C@H]12)OC(=O)[C@@H](C)CC)C[C@@H]1C[C@@H](O)CC(=O)O1 PCZOHLXUXFIOCF-BXMDZJJMSA-N 0.000 description 1
- 229960004844 lovastatin Drugs 0.000 description 1
- QLJODMDSTUBWDW-UHFFFAOYSA-N lovastatin hydroxy acid Natural products C1=CC(C)C(CCC(O)CC(O)CC(O)=O)C2C(OC(=O)C(C)CC)CC(C)C=C21 QLJODMDSTUBWDW-UHFFFAOYSA-N 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 229940035034 maltodextrin Drugs 0.000 description 1
- 235000021590 normal diet Nutrition 0.000 description 1
- 210000004279 orbit Anatomy 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 230000004792 oxidative damage Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 229940079877 pyrogallol Drugs 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- IKGXIBQEEMLURG-NVPNHPEKSA-N rutin Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-NVPNHPEKSA-N 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- FDRCDNZGSXJAFP-UHFFFAOYSA-M sodium chloroacetate Chemical compound [Na+].[O-]C(=O)CCl FDRCDNZGSXJAFP-UHFFFAOYSA-M 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 150000003626 triacylglycerols Chemical class 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 208000019553 vascular disease Diseases 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
A kind of health food with anti-oxidation function and its preparation method and application, is related to a kind of health food and its preparation method and application.Purpose is to provide a kind of new natural food with anti-oxidation function and its preparation method and application, has expanded the species of natural, while solve easily to lose antioxidation activity under existing natural high temperature.The health food includes European gooseberry extract, golden balsam pear seed extract, hazelnut polysaccharide and black nightshade polysaccharide.Method：The European gooseberry extract weighed, golden balsam pear seed extract, hazelnut polysaccharide and black nightshade polysaccharide are sufficiently mixed, then add ethanol, is stirred in mixer and softwood is made, pelletized, dried, whole grain, that is, obtain the health food with anti-oxidation function.The present invention pass through scientific matching, each component synergy, complementary mutually rush, the significantly more efficient ability to Green Tea Extract, the effect of can playing anti-oxidant, anti-aging.The present invention is used for antioxidant food field.
Description
Technical field
The present invention relates to a kind of health food and its preparation method and application.
Background technology
Anti-oxidant increasing research display is pre- anti-aging important step, because free radical or oxidant can lead to
Cross to the oxidative damage of human inner cell's tissue and a series of infringement is produced to organism, accelerate the aging course of body and lure
Send out disease various.Therefore, enough antioxidants should be absorbed, the generation of free radical is reduced or accelerates its removing, to resist freedom
The side effect of base, delays body aging.
People have found antioxidant, such as Tea Polyphenols, bioflavonoid, carotenoid, dimension life in natural food
Plain C, vitamin E etc., these antioxidants being derived from wholefood are safer, become the optimal selection of antioxidant.
How more antioxidants are obtained in wholefood, be that current researcher's emphasis will solve the problems, such as.
In addition, the problem of existing antioxidant generally existing non-refractory, they at high temperature (100 DEG C) can lose it is anti-
Oxidation activity.Its pot-life is also shorter, in the case where not adding the material that extends the shelf life, generally 7~10 days.
The content of the invention
It is an object of the invention to provide a kind of new natural food with anti-oxidation function and its preparation method and application,
The species of natural has been expanded, while has solved easily to lose antioxidation activity under existing natural high temperature, has been protected
Deposit the time limit it is short the problem of.
The present invention have anti-oxidation function health food by mass fraction include 50~70 parts European gooseberry extract,
70~90 parts of golden balsam pear seed extract, 5~8 parts of hazelnut polysaccharide and 5~10 parts of black nightshade polysaccharide.
Further, the preparation method of the European gooseberry extract, comprises the following steps：
European gooseberry fruit is taken, naturally dry after cleaning, homogenate is made with tissue pulper, homogenate is added into round-bottomed flask
In, ethyl acetate is added, 2~3h of heating and refluxing extraction, extract solution is filtered out after cooling, residue repeats extraction once, merges twice
Extract solution, recovery ethyl acetate obtain medicinal extract, be crushed to 60~80 mesh, obtain extract.
Further, the preparation method of the golden balsam pear seed extract, comprises the following steps：
Golden balsam pear is cut, wherein red golden balsam pear seed is taken, is put into -80 DEG C of refrigerator freezings and stays overnight, then freezed
Dry, grind into powder, obtains golden balsam pear seed freeze-dried powder afterwards；
Ethanol is added after to golden balsam pear seed freeze-dried powder, 60 DEG C of 2~3h of extraction is heated to, is filtrated to get ethanol extract,
Repeat to merge extract solution after extracting 2~3 times, be heated to 60 DEG C and vacuumize backflow recovery ethanol, then be extracted with ethyl acetate, will extract
Dissolved after taking the ethyl acetate recovery of liquid extremely with water, then by macroporous resin purification, with 60~80% ethanol elution, finally
Eluent is concentrated, dry, crushed, obtains extract.
Further, the preparation method of the hazelnut polysaccharide, comprises the following steps：
By hazelnut it is dry, crush, add petroleum ether, in 40~70 DEG C of heating and refluxing extractions 2~3 times, 2~5h every time, mistake
Recycling design is evaporated under reduced pressure in filter, merging filtrate at 50~60 DEG C, and filter residue obtains degreasing hazelnut by being dried under reduced pressure；
Degreasing hazelnut is added into distilled water, extracted 3 times in 80~90 DEG C of water-baths, merges extract solution, concentration, adds isometric
Trichloroacetic acid (15%), centrifuge after 12~14h, take supernatant, 95% ethanol of 3 times of volumes is added into supernatant, stand
Overnight, precipitation is collected by centrifugation, is washed successively with ether, absolute ethyl alcohol, totally 3~5 times, vacuum freeze-drying obtains powder, as hazelnut
Polysaccharide.
Further, the preparation method of the black nightshade polysaccharide, comprises the following steps：
Black nightshade herb chloroform-methanol (2:1) degreasing, decolourize, with 80% ethanol heating and refluxing extraction 2~3 times, after filtering
To residue；
Dried residue is taken, the distilled water for adding 15 times of amounts decocts 2 times, each 4h, merges extract solution, is concentrated under reduced pressure,
It is 80% to add 95% ethanol to final volume fraction of ethanol, and 4 DEG C stand overnight, and precipitation is collected by centrifugation, successively with ether, anhydrous
Ethanol washs, and totally 3~5 times, vacuum freeze-drying obtains powder, as black nightshade polysaccharide.
The preparation method of the above-mentioned health food with anti-oxidation function, comprises the following steps：
First, by mass fraction weigh 50~70 parts European gooseberry extract, 70~90 parts of golden balsam pear seed extract, 5
~8 parts of hazelnut polysaccharide and 5~10 parts of black nightshade polysaccharide；
2nd, the European gooseberry extract weighed, golden balsam pear seed extract, hazelnut polysaccharide and black nightshade polysaccharide are sufficiently mixed,
Then ethanol is added, is stirred in mixer and softwood is made, softwood pelletized with 16 eye mesh screens, then by particle at 60~70 DEG C
Lower drying to moisture is less than 3%, then with 14 eye mesh screen whole grains, and obtained particle is dispensed to after mixing in mixer, that is, obtained
Health food with anti-oxidation function.
Stabilizer and sweetener can be also added in the raw material of the above method, to increase mouthfeel and improve stability.It is described steady
Agent is determined for yellow glue original, starch Sodium Octenyl Succinate etc., and sweetener is stevioside, Sucralose, mogroside etc..
Application of the above-mentioned health food in the food and health products for preparing reducing blood lipid.
Beneficial effects of the present invention：
The present invention provides a kind of health-care food composition with anti-oxidation function, is confirmed through overtesting, said composition
The clearance rate of hydroxyl radical free radical reaches more than 95%, and the clearance rate of superoxide anion reaches more than 85%, H2O2Clearance rate reach
More than 90%.
The present invention passes through scientific matching, each component synergy, complementary mutually rush, the significantly more efficient energy to Green Tea Extract
Power, the effect of anti-oxidant, anti-aging can be played.The present invention composition can also reducing blood lipid, can effectively prevent the heart
Cranial vascular disease, preferably safeguard the health of cardio-cerebrovascular.
The present invention health-care food composition can high temperature resistant, at a high temperature of 121 DEG C, still there is higher anti-oxidant energy
Power.
The health-care food composition of the present invention has longer storage period at normal temperatures, is guaranteed the quality not adding any extension
On the premise of phase material, store 30~60 days, still there is higher oxidation resistance.
Embodiment
Technical solution of the present invention is not limited to act embodiment set forth below, in addition between each embodiment
Any combination.
Embodiment one：The health food that present embodiment has anti-oxidation function includes 50~70 by mass fraction
Part European gooseberry extract, 70~90 parts of golden balsam pear seed extract, 5~8 parts of hazelnut polysaccharide and 5~10 parts of black nightshade it is more
Sugar.
Embodiment two：Present embodiment is unlike embodiment one：The European gooseberry extract
Preparation method, comprise the following steps：
European gooseberry fruit is taken, naturally dry after cleaning, homogenate is made with tissue pulper, homogenate is added into round-bottomed flask
In, ethyl acetate is added, 2~3h of heating and refluxing extraction, extract solution is filtered out after cooling, residue repeats extraction once, merges twice
Extract solution, recovery ethyl acetate obtain medicinal extract, be crushed to 60~80 mesh, obtain extract.It is other with the phase of embodiment one
Together.
Embodiment three：Present embodiment is unlike embodiment one：The golden balsam pear seed extract
Preparation method, comprise the following steps：
Golden balsam pear is cut, wherein red golden balsam pear seed is taken, is put into -80 DEG C of refrigerator freezings and stays overnight, then freezed
Dry, grind into powder, obtains golden balsam pear seed freeze-dried powder afterwards；
Ethanol is added after to golden balsam pear seed freeze-dried powder, 60 DEG C of 2~3h of extraction is heated to, is filtrated to get ethanol extract,
Repeat to merge extract solution after extracting 2~3 times, be heated to 60 DEG C and vacuumize backflow recovery ethanol, then be extracted with ethyl acetate, will extract
Dissolved after taking the ethyl acetate recovery of liquid extremely with water, then by macroporous resin purification, with 60~80% ethanol elution, finally
Eluent is concentrated, dry, crushed, obtains extract.It is other identical with embodiment one.
Embodiment four：Present embodiment is unlike embodiment one：The preparation of the hazelnut polysaccharide
Method, comprise the following steps：
By hazelnut it is dry, crush, add petroleum ether, in 40~70 DEG C of heating and refluxing extractions 2~3 times, 2~5h every time, mistake
Recycling design is evaporated under reduced pressure in filter, merging filtrate at 50~60 DEG C, and filter residue obtains degreasing hazelnut by being dried under reduced pressure；
Degreasing hazelnut is added into distilled water, extracted 3 times in 80~90 DEG C of water-baths, merges extract solution, concentration, adds isometric
Mass concentration 15% trichloroacetic acid, centrifuge after 12~14h, take supernatant, 95% second of 3 times of volumes is added into supernatant
Alcohol, stand overnight, precipitation is collected by centrifugation, washed successively with ether, absolute ethyl alcohol, totally 3~5 times, vacuum freeze-drying obtains powder, i.e.,
For hazelnut polysaccharide.It is other identical with embodiment one.
Embodiment five：Present embodiment is unlike embodiment one：The preparation of the black nightshade polysaccharide
Method, comprise the following steps：
Black nightshade herb chloroform-methanol (2:1) degreasing, decolourize, with 80% ethanol heating and refluxing extraction 2~3 times, after filtering
To residue；
Dried residue is taken, the distilled water for adding 15 times of amounts decocts 2 times, each 4h, merges extract solution, is concentrated under reduced pressure,
It is 80% to add 95% ethanol to final volume fraction of ethanol, and 4 DEG C stand overnight, and precipitation is collected by centrifugation, successively with ether, anhydrous
Ethanol washs, and totally 3~5 times, vacuum freeze-drying obtains powder, as black nightshade polysaccharide.It is other identical with embodiment one.
Embodiment six：Present embodiment has the preparation method of the health food of anti-oxidation function, including following
Step：
First, by mass fraction weigh 50~70 parts European gooseberry extract, 70~90 parts of golden balsam pear seed extract, 5
~8 parts of hazelnut polysaccharide and 5~10 parts of black nightshade polysaccharide；
2nd, the European gooseberry extract weighed, golden balsam pear seed extract, hazelnut polysaccharide and black nightshade polysaccharide are sufficiently mixed,
Then ethanol is added, is stirred in mixer and softwood is made, softwood pelletized with 16 eye mesh screens, then by particle at 60~70 DEG C
Lower drying to moisture is less than 3%, then with 14 eye mesh screen whole grains, and obtained particle is dispensed to after mixing in mixer, that is, obtained
Health food with anti-oxidation function.
Embodiment seven：Present embodiment is unlike embodiment six：Stabilizer is additionally added in raw material,
The stabilizer is yellow glue original or starch Sodium Octenyl Succinate.It is other identical with embodiment six.
Embodiment eight：Present embodiment is unlike embodiment six or seven：Sweet tea is additionally added in raw material
Taste agent, the sweetener are stevioside, Sucralose or mogroside.It is other identical with embodiment six or seven.
Embodiment nine：Present embodiment is unlike embodiment six：The European gooseberry extract
Preparation method, comprise the following steps：
European gooseberry fruit is taken, naturally dry after cleaning, homogenate is made with tissue pulper, homogenate is added into round-bottomed flask
In, ethyl acetate is added, 2~3h of heating and refluxing extraction, extract solution is filtered out after cooling, residue repeats extraction once, merges twice
Extract solution, recovery ethyl acetate obtain medicinal extract, be crushed to 60~80 mesh, obtain extract.It is other with the phase of embodiment six
Together.
Embodiment ten：Present embodiment is unlike embodiment six：The golden balsam pear seed extract
Preparation method, comprise the following steps：
Golden balsam pear is cut, wherein red golden balsam pear seed is taken, is put into -80 DEG C of refrigerator freezings and stays overnight, then freezed
Dry, grind into powder, obtains golden balsam pear seed freeze-dried powder afterwards；
Ethanol is added after to golden balsam pear seed freeze-dried powder, 60 DEG C of 2~3h of extraction is heated to, is filtrated to get ethanol extract,
Repeat to merge extract solution after extracting 2~3 times, be heated to 60 DEG C and vacuumize backflow recovery ethanol, then be extracted with ethyl acetate, will extract
Dissolved after taking the ethyl acetate recovery of liquid extremely with water, then by macroporous resin purification, with 60~80% ethanol elution, finally
Eluent is concentrated, dry, crushed, obtains extract.It is other identical with embodiment six.
Embodiment 11：Present embodiment is unlike embodiment six：The system of the hazelnut polysaccharide
Preparation Method, comprise the following steps：
By hazelnut it is dry, crush, add petroleum ether, in 40~70 DEG C of heating and refluxing extractions 2~3 times, 2~5h every time, mistake
Recycling design is evaporated under reduced pressure in filter, merging filtrate at 50~60 DEG C, and filter residue obtains degreasing hazelnut by being dried under reduced pressure；
Degreasing hazelnut is added into distilled water, extracted 3 times in 80~90 DEG C of water-baths, merges extract solution, concentration, adds isometric
Mass concentration 15% trichloroacetic acid, centrifuge after 12~14h, take supernatant, 95% second of 3 times of volumes is added into supernatant
Alcohol, stand overnight, precipitation is collected by centrifugation, washed successively with ether, absolute ethyl alcohol, totally 3~5 times, vacuum freeze-drying obtains powder, i.e.,
For hazelnut polysaccharide.It is other identical with embodiment six.
Embodiment 12：Present embodiment is unlike embodiment six：The system of the black nightshade polysaccharide
Preparation Method, comprise the following steps：
Black nightshade herb chloroform-methanol (2:1) degreasing, decolourize, with 80% ethanol heating and refluxing extraction 2~3 times, after filtering
To residue；
Dried residue is taken, the distilled water for adding 15 times of amounts decocts 2 times, each 4h, merges extract solution, is concentrated under reduced pressure,
It is 80% to add 95% ethanol to final volume fraction of ethanol, and 4 DEG C stand overnight, and precipitation is collected by centrifugation, successively with ether, anhydrous
Ethanol washs, and totally 3~5 times, vacuum freeze-drying obtains powder, as black nightshade polysaccharide.It is other identical with embodiment six.
Embodiment 13：The health food that present embodiment has anti-oxidation function is preparing the food of reducing blood lipid
And the application in health products.
Embodiments of the invention are elaborated below, following examples are entered under premised on technical solution of the present invention
Row is implemented, and gives detailed embodiment and specific operating process, but protection scope of the present invention is not limited to following realities
Apply example.
Embodiment 1：
The present embodiment has the health-care food composition of anti-oxidation function by 600g Europe gooseberry extract, 800g gold balsam pears
Seed extract, 50g hazelnuts polysaccharide and 70g black nightshades polysaccharide composition.
The preparation method of wherein described European gooseberry extract, comprises the following steps：
European gooseberry fruit is taken, naturally dry after cleaning, homogenate is made with tissue pulper, homogenate is added into round-bottomed flask
In, ethyl acetate is added, heating and refluxing extraction 2h filters out extract solution after cooling, residue repeats extraction once, merges carrying twice
Liquid is taken, recovery ethyl acetate obtains medicinal extract, is crushed to 60 mesh, obtains European gooseberry extract.
The preparation method of the golden balsam pear seed extract, comprises the following steps：
Golden balsam pear is cut, wherein red golden balsam pear seed is taken, is put into -80 DEG C of refrigerator freezings and stays overnight, then freezed
Dry, grind into powder, obtains golden balsam pear seed freeze-dried powder afterwards；
Ethanol is added after to golden balsam pear seed freeze-dried powder, 60 DEG C of extraction 2h is heated to, is filtrated to get ethanol extract, repeats
Merge extract solution after extracting 3 times, be heated to 60 DEG C and vacuumize backflow recovery ethanol, then be extracted with ethyl acetate, by extract extremely
Ethyl acetate recovery after dissolved with water, it is finally that eluent is dense with 80% ethanol elution then by macroporous resin purification
Contracting, dry, crush, obtaining golden balsam pear seed extract.
The preparation method of the hazelnut polysaccharide, comprises the following steps：
By hazelnut drying, crush, add petroleum ether, in 50 DEG C of heating and refluxing extractions 3 times, each 2h, filtering, merge and filter
Liquid, is evaporated under reduced pressure recycling design at 60 DEG C, and filter residue obtains degreasing hazelnut by being dried under reduced pressure；
Degreasing hazelnut is added into distilled water, extracted 3 times in 80 DEG C of water-baths, merges extract solution, concentration, adds isometric three
Monoxone (15%), centrifuge after 12h, take supernatant, 95% ethanol of 3 times of volumes is added into supernatant, is stood overnight, centrifuge
Precipitation is collected, is washed successively with ether, absolute ethyl alcohol, totally 3 times, vacuum freeze-drying obtains powder, as hazelnut polysaccharide.
The preparation method of the black nightshade polysaccharide, comprises the following steps：
Black nightshade herb chloroform-methanol (2:1) degreasing, decolourize, with 80% ethanol heating and refluxing extraction 3 times, obtained after filtering
Residue；
Dried residue is taken, the distilled water for adding 15 times of amounts decocts 2 times, each 4h, merges extract solution, is concentrated under reduced pressure,
It is 80% to add 95% ethanol to final volume fraction of ethanol, and 4 DEG C stand overnight, and precipitation is collected by centrifugation, successively with ether, anhydrous
Ethanol washs, and totally 3 times, vacuum freeze-drying obtains powder, as black nightshade polysaccharide.
First, the health-care food composition of the present embodiment is added into distilled water to appropriate concentration, health-care food composition is entered
Row determination oxidative, it is specific as follows：
(1) hydroxyl radical free radical ability measure is removed
Sample cell：2mL Phen solution is taken, phosphate solution (pH7.4) 3mL is added thereto, is added after mixing
FeSO4Solution 1mL, 1.5mL composition solutions are added after fully mixing, add ultra-pure water afterwards, finally add 2mL H2O2Mixing is equal
It is even, after insulation reaction 1h, numerical value is determined under 536nm.
Blank tube：Composition solution and H are replaced with ultra-pure water2O2Solution, assay method are same as above.
Damage pipe：Composition solution is replaced with ultra-pure water, assay method is same as above, and is set 3 repetitions and averaged.
Result of the test：
Stronger to the Scavenging activity of hydroxyl radical free radical, when concentration is 15mg/mL, the clearance rate of hydroxyl radical free radical reaches
95.23%, higher than the clearance rate (92.89%) of Vc under equal experiment condition.
(2) superoxide anion ability measure is removed
4mL composition solutions are taken, add 5mL Tris-HCl buffer solutions (pH8.2) thereto, are mixed after being protected in water-bath
Warm 15min, 0.15mL pyrogallol solution is then added, its reaction is terminated with 0.8mLHCl solution afterwards, under 335nm wavelength
Measure.Wherein by the use of ultra-pure water as control, by the use of Tris buffer solutions as blank, it is repeated 3 times experiment and averages.
Result of the test：
Stronger to the Scavenging activity of superoxide anion, when concentration is 15mg/mL, the clearance rate of superoxide anion reaches
87.35%, higher than the clearance rate (85.13%) of Vc under equal experiment condition.
(3) H is removed2O2The measure of ability
Take pH7.4 phosphate buffers that composition solution is diluted into experimental concentration, equally take phosphate buffer by H2O2
Solution is diluted to 40mmol/L.The composition solution 3mL after dilution is taken afterwards, with 0.6mL H2O2Solution fully mixes, after standing
Its light absorption value is determined under 230nm wavelength, wherein with H2O2The light absorption value of solution itself is repeated 3 times experiment and is averaged as control
Value.
Result of the test：
To H2O2Scavenging activity it is stronger, when concentration is 15mg/mL, H2O2Clearance rate reach 90.38%, with equal reality
Vc clearance rate (90.76%) is suitable under the conditions of testing.
2nd, food safety evaluation is carried out to the present embodiment health food, it is specific as follows：
(1) test material
Sample：The present embodiment is had after the health food of anti-oxidation function is dissolved in distilled water, be configured to needed for it is dense
Degree, 4 DEG C of preservations, heating water bath is to 37 DEG C before administration.
Animal subject：The ICR Strains of Mouse of health, 18~22g of body weight, male and female half and half.
(2) test method
Before and after feeding the present embodiment health food, experiment mice male and female divide cage, and full-valence pellet feed is fed, free water,
20~25 DEG C of room temperature, laboratory relative humidity are 50%~70%.
Mouse is randomly divided into 2 groups：(1) the present embodiment health food group；(2) blank control group.Fasting 12h before experiment,
With 0.5g/mL the present embodiment health food, the gastric infusion 0.5mL/10g once a day；Blank control group gives equivalent
Distilled water.Continuous Observation 14d is not dead small situations such as record test mice behavior, activity, food ration, body weight, excrement, death
Mouse performs an autopsy on sb. after 14d.
Result of the test：
This experiment feeds mouse with maximum dosage-feeding, and the administration same day sees that mouse hair color is smooth, and activity, food ration, excrement are equal
Normally, second day activity, food ration, body weight increase, excrement etc. are normal, the no significant difference compared with blank control group, and two
Performed an autopsy on sb. put to death without dead and other abnormal generations, two groups in all simultaneously after, each main organs (heart, liver, spleen, lung, kidney) warp
Visually observe no abnormality seen change, the no significant difference compared with blank control group.
Toxicity assessment：
The maximum dosage-feeding result of the test that the present invention has the health food gastric infusion of anti-oxidation function shows, used small
Without obvious toxic reaction after mouse administration, without death in 2 weeks.Therefore the health food edible safety of the present invention.
Embodiment 2：
The present embodiment has the health-care food composition of anti-oxidation function by 500g Europe gooseberry extract, 700g gold balsam pears
Seed extract, 80g hazelnuts polysaccharide and 60g black nightshades polysaccharide composition.
Wherein European gooseberry extract, golden balsam pear seed extract, the same implementation of the preparation method of hazelnut polysaccharide and black nightshade polysaccharide
Example 1.
(1) antioxidation activity in vitro experiment is carried out to the health-care food composition of the present embodiment, to the present embodiment each component
Collaboration antioxidation verified, it is specific as follows：
Comparative example 1：500g Europe gooseberry extract is replaced with 500g maltodextrins, other components are same as Example 2.
Comparative example 2：700g gold balsam pear seed extracts are replaced with 700g maltodextrins, other components are same as Example 2.
Comparative example 3：80g hazelnut polysaccharide is replaced with 80g maltodextrins, other components are same as Example 2.
Comparative example 4：60g black nightshade polysaccharide is replaced with 60g maltodextrins, other components are same as Example 2.
Comparative example 5：The composition of the preparation of embodiment 2 is replaced with maltodextrin.
Equal amounts of compositions is taken respectively, is dissolved with 30% ethanol solution, is determined it respectively and is removed DPPH and ABTS+Activity
And TAC (rat blood serum and TAC kit be to be commercially available), its result is as shown in table 1.
Table 1
Each component synergy in the composition of the present invention, promotes mutually, shows to show it can be seen from the result of table 1
The oxidation resistance of work, anti-oxidation efficacy are significantly better than comparative example.
(2) high temperature resistant experiment is carried out
By the health food of the present embodiment be respectively placed in room temperature, 60 DEG C, under 121 DEG C of environment temperature, determine its removing
DPPH and ABTS+Activity and TAC (rat blood serum and TAC kit be to be commercially available), its
As a result it is as shown in table 2.
Table 2
The composition of the present invention still has higher anti-oxidant energy at a high temperature of 121 DEG C it can be seen from the result of table 2
Power, illustrate that the health food of the present invention being capable of high temperature resistant.
(3) long-term storage test is carried out
After the health food of the present embodiment is stored 10 days, 15 days, 30 days respectively at normal temperatures, determine it and remove DPPH
And ABTS+Activity and TAC (rat blood serum and TAC kit be to be commercially available), its result
As shown in table 3.
Table 3
The composition of the present invention stores 30~60 days at normal temperatures it can be seen from the result of table 2, still has higher antioxygen
Change ability, illustrating the health food of the present invention has longer storage period.
Embodiment 3：
The present embodiment has the health-care food composition of anti-oxidation function by 600g Europe gooseberry extract, 900g gold balsam pears
Seed extract, 50g hazelnuts polysaccharide and 100g black nightshades polysaccharide composition.
Wherein European gooseberry extract, golden balsam pear seed extract, the same implementation of the preparation method of hazelnut polysaccharide and black nightshade polysaccharide
Example 1.
(1) animal
ICR mouse, male and female half and half, 18~22g of body weight.
(2) method
Mouse is randomly divided into Normal group and high lipid food group, Normal group animal is used from on-test to end
Normal diet is fed, and remaining animal feeds high lipidol feed (high lipid food formula：Cholesterol 1%, yolk powder 10%, lard
10%th, basal feed 79%), body weight is measured after 3 weeks, high lipid food group the weight of animals is higher than Normal group, examined through t, number
According to difference tool significance (being shown in Table 4), it is determined that hyperlipemia model has been formed, then high lipid food group animal is random by body weight
It is divided into model control group, positive drug control group, composition high dose group, composition middle dose group, composition low dose group, animal
Continue to give high lipid food, model control group gives the solvent of respective volume daily, and composition high dose group is with 45mg/kgd
Dosage be combined thing gastric infusion, composition middle dose group is combined thing gastric infusion with 15mg/kgd dosage,
Composition low dose group is combined thing gastric infusion with 5mg/kgd dosage, and positive drug control group gives 5mg/kgd's
Lovastatin, Normal group give isometric solvent.After 20d, the equal fasting 12h of each group mouse, eye socket takes blood, and heparin resists
Solidifying, anticoagulated blood centrifugal separation plasma is due to lipid determination.Wherein LDL-C, HDL-C, TC, TG measure saying according to kit
Bright method is carried out, as a result as shown in table 4.
Table 4
Remarks：Compared with model control group, * p<0.05, * * p<0.01.
Result of the test shows, composition administration group, positive drug control group blood plasma in TG, TC, LDL-C compared with model comparison
Group decreases, data difference significance.In the blood plasma of each administration group of composition and positive drug control group HDL-C compared with
Model control group has raised, data difference significance.High blood for money can be reduced by illustrating the composition of the present embodiment
The indexs such as the total plasma cholesterol of fat mouse, total triglycerides, LDL-C, increasing high density lipoprotein courage are consolidated
Alcohol level, there is preferable effect for reducing blood fat.
Claims (9)
1. a kind of health food with anti-oxidation function, it is characterised in that the health food includes 50~70 parts by mass fraction
European gooseberry extract, 70~90 parts of golden balsam pear seed extract, 5~8 parts of hazelnut polysaccharide and 5~10 parts of black nightshade it is more
Sugar.
A kind of 2. health food with anti-oxidation function according to claim 1, it is characterised in that the European gooseberry
The preparation method of extract, comprises the following steps：
European gooseberry fruit is taken, naturally dry after cleaning, homogenate is made with tissue pulper, homogenate is added in round-bottomed flask,
Ethyl acetate is added, 2~3h of heating and refluxing extraction filters out extract solution after cooling, residue repeats extraction once, merges carrying twice
Liquid is taken, recovery ethyl acetate obtains medicinal extract, is crushed to 60~80 mesh, obtains European gooseberry extract.
A kind of 3. health food with anti-oxidation function according to claim 1, it is characterised in that the golden balsam pear seed
The preparation method of extract, comprises the following steps：
1. golden balsam pear is cut, wherein red golden balsam pear seed is taken, -80 DEG C of refrigerator freezings is put into and stays overnight, it is dry then to carry out freezing
Dry, grind into powder, obtains golden balsam pear seed freeze-dried powder afterwards；
2. adding ethanol after to golden balsam pear seed freeze-dried powder, 60 DEG C of 2~3h of extraction are heated to, are filtrated to get ethanol extract, weight
Merge extract solution after extracting 2~3 times again, be heated to 60 DEG C and vacuumize backflow recovery ethanol, then be extracted with ethyl acetate, will extract
Dissolved with water after the ethyl acetate recovery of liquid extremely, then by macroporous resin purification, with 60~80% ethanol elution, finally will
Eluent concentration, dry, crush, obtaining golden balsam pear seed extract.
A kind of 4. health food with anti-oxidation function according to claim 1, it is characterised in that the hazelnut polysaccharide
Preparation method, comprise the following steps：
1. by hazelnut it is dry, crush, add petroleum ether, in 40~70 DEG C of heating and refluxing extractions 2~3 times, 2~5h every time, filtering,
Merging filtrate, is evaporated under reduced pressure recycling design at 50~60 DEG C, and filter residue obtains degreasing hazelnut by being dried under reduced pressure；
2. degreasing hazelnut is added into distilled water, extracted 3 times in 80~90 DEG C of water-baths, merge extract solution, concentration, add isometric
The trichloroacetic acid of mass concentration 15%, centrifuge after 12~14h, take supernatant, 95% second of 3 times of volumes is added into supernatant
Alcohol, stand overnight, precipitation is collected by centrifugation, washed successively with ether, absolute ethyl alcohol, totally 3~5 times, vacuum freeze-drying obtains powder, i.e.,
For hazelnut polysaccharide.
A kind of 5. health food with anti-oxidation function according to claim 1, it is characterised in that the black nightshade polysaccharide
Preparation method, comprise the following steps：
1. the chloroform-methanol degreasing of black nightshade herb, decolourizing, with 80% ethanol heating and refluxing extraction 2~3 times, obtained after filtering residual
Slag；
2. taking dried residue, the distilled water for adding 15 times of amounts decocts 2 times, each 4h, merges extract solution, is concentrated under reduced pressure, adds
It is 80% to enter 95% ethanol to final volume fraction of ethanol, and 4 DEG C stand overnight, and precipitation is collected by centrifugation, successively with ether, anhydrous second
Alcohol washs, and totally 3~5 times, vacuum freeze-drying obtains powder, as black nightshade polysaccharide.
6. the preparation method of the health food with anti-oxidation function as claimed in claim 1, it is characterised in that this method bag
Include following steps：
First, by mass fraction weigh 50~70 parts European gooseberry extract, 70~90 parts of golden balsam pear seed extract, 5~8 parts
Hazelnut polysaccharide and 5~10 parts of black nightshade polysaccharide；
2nd, the European gooseberry extract weighed, golden balsam pear seed extract, hazelnut polysaccharide and black nightshade polysaccharide are sufficiently mixed, then
Ethanol is added, is stirred in mixer and softwood is made, softwood is pelletized with 16 eye mesh screens, then does particle at 60~70 DEG C
It is dry to be less than 3% to moisture, then with 14 eye mesh screen whole grains, obtained particle is dispensed to after mixing in mixer, that is, had
The health food of anti-oxidation function.
7. the preparation method of the health food according to claim 6 with anti-oxidation function, it is characterised in that in raw material
Stabilizer is additionally added, the stabilizer is yellow glue original or starch Sodium Octenyl Succinate.
8. the preparation method of the health food according to claim 6 with anti-oxidation function, it is characterised in that in raw material
Sweetener is additionally added, the sweetener is stevioside, Sucralose or mogroside.
9. application of the health food with anti-oxidation function in the food and health products for preparing reducing blood lipid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710672083.2A CN107455741A (en) | 2017-08-08 | 2017-08-08 | A kind of health food with anti-oxidation function and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710672083.2A CN107455741A (en) | 2017-08-08 | 2017-08-08 | A kind of health food with anti-oxidation function and its preparation method and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107455741A true CN107455741A (en) | 2017-12-12 |
Family
ID=60547686
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710672083.2A Pending CN107455741A (en) | 2017-08-08 | 2017-08-08 | A kind of health food with anti-oxidation function and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107455741A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109875083A (en) * | 2019-04-11 | 2019-06-14 | 广西壮族自治区农业科学院 | A kind of preparation method of Sweet Potato Leaf lozenge |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102204946A (en) * | 2011-05-24 | 2011-10-05 | 河南中医学院 | Chinese lobelia polysaccharide extracted from Chinese lobelia and application thereof |
| CN102258196A (en) * | 2010-12-24 | 2011-11-30 | 天津天狮生物发展有限公司 | Health-care food with anti-oxidation function |
| CN102351956A (en) * | 2011-08-09 | 2012-02-15 | 朱孟勇 | Extraction method of morindea officinalis polysaccharide |
| CN102757510A (en) * | 2012-04-28 | 2012-10-31 | 江苏大学 | Low-molecular weight schisandra chinensis polysaccharides and preparation method and application thereof |
| CN103652859A (en) * | 2013-11-13 | 2014-03-26 | 西安医学院 | Health-care food with oxidation resistance and preparation method thereof |
| CN104857127A (en) * | 2015-06-15 | 2015-08-26 | 青岛华仁技术孵化器有限公司 | Oral administration medicine used for curing accumulated damp heat type chronic pelvic inflammatory disease |
| CN104922470A (en) * | 2015-05-28 | 2015-09-23 | 许江 | Antioxidative fat-reducing composition and preparation method thereof |
| CN104974271A (en) * | 2015-07-20 | 2015-10-14 | 中国林业科学研究院林产化学工业研究所 | Preparation method of rhus verniciflua stoke seed polysaccharide with antioxidant activity |
-
2017
- 2017-08-08 CN CN201710672083.2A patent/CN107455741A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102258196A (en) * | 2010-12-24 | 2011-11-30 | 天津天狮生物发展有限公司 | Health-care food with anti-oxidation function |
| CN102204946A (en) * | 2011-05-24 | 2011-10-05 | 河南中医学院 | Chinese lobelia polysaccharide extracted from Chinese lobelia and application thereof |
| CN102351956A (en) * | 2011-08-09 | 2012-02-15 | 朱孟勇 | Extraction method of morindea officinalis polysaccharide |
| CN102757510A (en) * | 2012-04-28 | 2012-10-31 | 江苏大学 | Low-molecular weight schisandra chinensis polysaccharides and preparation method and application thereof |
| CN103652859A (en) * | 2013-11-13 | 2014-03-26 | 西安医学院 | Health-care food with oxidation resistance and preparation method thereof |
| CN104922470A (en) * | 2015-05-28 | 2015-09-23 | 许江 | Antioxidative fat-reducing composition and preparation method thereof |
| CN104857127A (en) * | 2015-06-15 | 2015-08-26 | 青岛华仁技术孵化器有限公司 | Oral administration medicine used for curing accumulated damp heat type chronic pelvic inflammatory disease |
| CN104974271A (en) * | 2015-07-20 | 2015-10-14 | 中国林业科学研究院林产化学工业研究所 | Preparation method of rhus verniciflua stoke seed polysaccharide with antioxidant activity |
Non-Patent Citations (3)
| Title |
|---|
| 李冠业: "龙葵多糖分离纯化、结构鉴定及抗H22肿瘤活性研究", 《中国优秀硕士学位论文全文数据库》 * |
| 李莉等: "新疆黑加仑提取物体外抗氧化活性的研究", 《食品与发酵工业》 * |
| 王磊等: "TCA法脱除榛子壳多糖中蛋白质的工艺研究", 《农产品加工》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109875083A (en) * | 2019-04-11 | 2019-06-14 | 广西壮族自治区农业科学院 | A kind of preparation method of Sweet Potato Leaf lozenge |
| CN109875083B (en) * | 2019-04-11 | 2021-05-07 | 广西壮族自治区农业科学院 | Preparation method of sweet potato leaf buccal tablets |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Fuentes-Alventosa et al. | Preparation of bioactive extracts from asparagus by-product | |
| Bacha et al. | Nutraceutical, anti-inflammatory, and immune modulatory effects of β-glucan isolated from yeast | |
| Zhang et al. | Changes in bio‐accessibility, polyphenol profile and antioxidants of quinoa and djulis sprouts during in vitro simulated gastrointestinal digestion | |
| Kosmala et al. | Chemical composition of natural and polyphenol-free apple pomace and the effect of this dietary ingredient on intestinal fermentation and serum lipid parameters in rats | |
| Sudha et al. | Mango pulp fibre waste: characterization and utilization as a bakery product ingredient | |
| Costamagna et al. | Nutritional and antioxidant properties of Geoffroea decorticans, an Argentinean fruit, and derived products (flour, arrope, decoction and hydroalcoholic beverage) | |
| CN103652859B (en) | A kind of health food with anti-oxidation function and preparation method thereof | |
| WO2005115422A2 (en) | Compositions containing avocado leaf extract for lowering cholesterol levels | |
| Lim et al. | Potential functional byproducts from guava puree processing | |
| CN101505775B (en) | Antioxidant composition containing component originating in the bark of tree belonging to the genus acacia | |
| CN101505776B (en) | Antiobesity composition containing component originating in the bark of tree belonging to the genus acacia | |
| JP6042800B2 (en) | Tomatoside A extraction method | |
| JP2005350453A (en) | Slightly water-soluble component of leaf of lagerstroemia speciosa l. | |
| CN107455741A (en) | A kind of health food with anti-oxidation function and its preparation method and application | |
| Samsudin et al. | THE EFFECT OF PACITAN’S SWEET ORANGE’S (Citrus sinensis (L) Osbeck) PEEL POWDER ON THE LIPID PROFILE OF MALE DYSLIPIDEMIA RATS Rattus novergicus | |
| CN105638934A (en) | Gingko compound edible oil | |
| CN101984994A (en) | Medicament for improving human intestinal flora balance and preparation method thereof | |
| KR101478196B1 (en) | Composition for the prevention of foods from oxidation and for retarding the onset of diabetes comprsing of the extract of Cudrania tricuspidata as a main component | |
| KR20150111786A (en) | Manufacturing method for Sunsik using Jeungpo (steamed and dried) method and Sunsik prepared by the method | |
| WO2013093161A1 (en) | Antioxidant ingredient with low calorie content, method for obtaining same and use thereof | |
| Ezeokonkwo Mercy et al. | Proximate composition and mineral analysis of Mucuna utilis (Velvet Bean) | |
| KR20110037020A (en) | The anti-diabetes composition containing silkworm culturing product and medicinal herbs | |
| CN101637207B (en) | Puer tea pigment with antilipemic activity and application thereof | |
| KR101808385B1 (en) | Roast grain powder containing red jinseng ingredient and method for prepairing the same | |
| WO2009141834A2 (en) | Process for preparing a bioactive fraction from red onion |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171212 |
|
| RJ01 | Rejection of invention patent application after publication |