CN101637207B - Puer tea pigment with antilipemic activity and application thereof - Google Patents
Puer tea pigment with antilipemic activity and application thereof Download PDFInfo
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- CN101637207B CN101637207B CN2009100940213A CN200910094021A CN101637207B CN 101637207 B CN101637207 B CN 101637207B CN 2009100940213 A CN2009100940213 A CN 2009100940213A CN 200910094021 A CN200910094021 A CN 200910094021A CN 101637207 B CN101637207 B CN 101637207B
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Abstract
The invention provides a puer theabrownin with antilipemic activity and an application thereof, belonging to the technical field of tea processing. The steps to obtain the theabrownin are as follows: smashing the puer ripe tea to 40 meshes, adding edible alcohol by 1g/50ml, standing for 6 hours in room temperature and collecting tea leafs; adding the tea leafs by 1g/100ml into deionized water at the temperature of 25 DEG C, preserving heat for 12 hours and collecting filter liquor; adding the tea leafs by 1g/100ml into 25 DEG C of deionized water, preserving heat for 12 hours, filtering and combining the filter liquors of the two times; mixing the combined filter liquor with edible alcohol according to a ratio of 1:4 and stirring for 10 min, stewing for 5 hours and collecting the precipitate; adding the precipitate by 1g/50ml into the 80% of edible alcohol water solution, stirring for 10 min and preserving heat for 3 hours at the temperature of 35 DEG C, collecting the filter liquor, adding the remaining precipitate by 1g/100ml repeatedly into 40% of edible alcohol water solution, stirring for 20 min, preserving heat for 3 hours at the temperature of 35 DEG C, collecting the filter liquor and combining the filter liquors of the two times; and concentrating and drying the filter liquor to obtain the theabrownine. The invention has lipid decreasing effect and can be applied to the fields of food and health care products.
Description
Technical field:
The present invention relates to a kind of theabrownin of Pu'er tea and application thereof, belong to the tea processing technique field with hypolipidemic activity.
Background technology:
Pu'er tea be meant ground such as Xishuangbanna, Yunnan Province, Simao, Lincang, the Baoshan, Kunming, ShiShimonoseki with big-leaf species in yunnan shine dark green gross tea through refining arrangement or steam molded after the product that obtains of storage and aging and having innovated through technological improvement since the seventies in 20th century all the year round, adopt big-leaf species in yunnan to shine the product that dark green gross tea makes through the wet heap of humidification and after-ripening ageing, have the dark brown damp brown of doing, the anti-bubble of stout and strong heavy real, the anti-storage of bar rope, liquor color red dense bright, Chen Xiang is remarkable, dense time of flavour is sweet, the characteristics of brown softness at the bottom of the leaf.Pu'er tea is loved by the people with the health-care efficacy of its unique processing process and reducing blood lipid, fat-reducing, warm stomach.That passes through us studies have shown that the higher theabrownin class material of content is the most important composition of its reducing blood lipid in the Pu'er tea.Usually, theabrownin be defined as a class can be water-soluble and be insoluble to the brown pigment of ethyl acetate and n-butanol.They are the very complicated compounds of a class,, the condensation product poly-except that the oxidation that contains Polyphenols, also contain bonds such as protein, amino acid, carbohydrate, nucleic acid, are formed by theaflavin and the plain further oxidation polymerization of congo red.Toxicological evaluation is the result show, theabrownin class material is actual nontoxic level material, and more safer than Tea Polyphenols, Tea Polyphenols belongs to the moderate toxicity material.These results show that theabrownin has application promise in clinical practice.
The present invention mainly from Pu'er tea preparation have a high theabrownin of hypolipidemic activity, and prove that by functional experiment theabrownin of the present invention has significant lipopenicillinase and prevention mouse effect on hyperlipemia to the hyperlipidemia mouse.Resultant theabrownin can be widely used in bread and cheese, health food, medicine, even chemical field.
By literature search, do not see the open report identical with the present invention.
Summary of the invention:
The object of the present invention is to provide a kind of theabrownin of Pu'er tea and application thereof with hypolipidemic activity.
The present invention prepares from the yunnan puer tea theabrownin has the high theabrownin of hypolipidemic activity, prepared product has significant lipopenicillinase and prevention mouse effect on hyperlipemia by the functional experiment proof to the hyperlipidemia mouse, resultant theabrownin can be widely used in food, medicine, even chemical field.
Theabrownin of the present invention obtains through following method:
A. Pu'er cooked tea being crushed to 40 orders, is the ratio throwing tea of 1g/50ml in tealeaves/edible alcohol, and room temperature is placed 6h, and constantly stirs, and tea grounds is collected in 300 order filter cloth centrifugal filtrations;
B. in tea grounds/deionized water the ratio input of 1g/100ml, 25 ℃ of water temperatures, insulation 12h with 300 order filter cloth centrifugal filtrations, collects filtrate; Be the ratio throwing tea of 1g/100ml again in tea grounds/deionized water, 25 ℃ of water temperatures, insulation 12h with 300 order filter cloth centrifugal filtrations, merges twice filtrate;
C. be 1: 4 with merging filtrate by filtrate/edible alcohol, unit is that the ratio of v/v is mixed and stirring 10min, leaves standstill 5h, centrifugal collecting precipitate;
D. sediment adds 80% edible alcohol water solution in the ratio of 1g/50ml, and alcohol/water v/v stirs 35 ℃ of insulation 3h behind the 10mi n, centrifugal collection purifying sediment;
E. with the edible alcohol water solution of purifying sediment in the ratio adding 40% of 1g/100ml, alcohol/water v/v, 35 ℃ of insulation 3h behind the stirring 20min, brownish red filtrate is collected in centrifugal filtration, remaining sediment repeats the edible alcohol water solution in the ratio adding 40% of 1g/100ml again, alcohol/water v/v, 35 ℃ of insulation 3h behind the stirring 20min, centrifugal filtration, collect brownish red filtrate, merge filtrate twice;
F. the filtrate that E step is obtained is after the vacuum decompression of routine concentrates, and vacuum drying promptly obtains having the theabrownin of hypolipidemic activity.
The theabrownin that the present invention is obtained carries out functional experiment, and its result is as follows:
Table 1 functional experiment partial results
The principal character of the theabrownin for preparing is: brownish red or chocolate brown powder, and soluble in water, be insoluble in organic solvents such as ethanol, methyl alcohol; MS the analysis showed that through mass spectrum, and its molecular weight is in the scope of 400-2000.Infrared spectrum analysis shows that it is the oxidation polymerization product of polyphenols.Contain 17 seed amino acids in its acid hydrolysis sample.Show that through functional experiment hyperlipidemia Kunming kind mouse is had significant lipopenicillinase and prevention mouse effect on hyperlipemia.Resultant theabrownin can be widely used in food and field of health care products.
It is easy that the present invention has the preparation method, and cost is low, and the amino acid content height has significant effect for reducing fat, can be widely used in food and field of health care products.
Description of drawings:
Fig. 1 is mass spectrum (MS) figure (molecular weight 733) of key component in the extract.
Fig. 2 is mass spectrum (MS) figure (molecular weight 685) of key component in the extract.
Fig. 3 is mass spectrum (MS) figure (molecular weight 481) of key component in the extract.
The mass spectrum of key component (MS) figure (molecular weight 1012) in Fig. 4 extract.
Fig. 5 extract infrared spectrogram (showing that such material is the aldehydes matter oxidation product).
Fig. 6 amino acid is analyzed collection of illustrative plates automatically.
The specific embodiment:
Example 1: in Application in Food
Theabrownin of the present invention obtains through following method:
A. Pu'er cooked tea being crushed to 40 orders, is the ratio throwing tea of 1g/50ml in tealeaves/edible alcohol, and room temperature is placed 6h, and constantly stirs, and tea grounds is collected in 300 order filter cloth centrifugal filtrations;
B. in tea grounds/deionized water the ratio input of 1g/100ml, 25 ℃ of water temperatures, insulation 12h with 300 order filter cloth centrifugal filtrations, collects filtrate; Be the ratio input of 1g/100ml again in tea grounds/deionized water, 25 ℃ of water temperatures, insulation 12h with 300 order filter cloth centrifugal filtrations, merges twice filtrate;
C. be 1: 4 with merging filtrate by filtrate/edible alcohol, unit is that the ratio of v/v is mixed and stirring 10min, leaves standstill 5h, centrifugal collecting precipitate;
D. sediment adds 80% edible alcohol water solution in the ratio of 1g/50ml, and alcohol/water v/v stirs 35 ℃ of insulation 3h behind the 10min, centrifugal collection purifying sediment;
E. with the edible alcohol water solution of purifying sediment in the ratio adding 40% of 1g/100ml, alcohol/water v/v, 35 ℃ of insulation 3h behind the stirring 20min, brownish red filtrate is collected in centrifugal filtration, remaining sediment repeats the edible alcohol water solution in the ratio adding 40% of 1g/100ml again, alcohol/water v/v, 35 ℃ of insulation 3h behind the stirring 20min, centrifugal filtration, collect brownish red filtrate, merge filtrate twice;
F. the filtrate that E step is obtained is after the vacuum decompression of routine concentrates, and vacuum drying promptly obtains having the theabrownin of hypolipidemic activity.
The theabrownin that above-mentioned steps is obtained adds among maltodextrin (20-35%), xylitol (10-25%), isomalt (15-20%), the smart powder (10-20%) of Yunnan olive, sodium carboxymethylcellulose (1-5%), konjac oligosaccharides (5-10%), the honey powder 1-5%, by adding 1.0-10.0g in the 100g mix, the tabletting method according to routine makes tablet again.
The theabrownin that above-mentioned steps is obtained adds among maltodextrin (8-15%), xylitol (25-40%), antierythrite (20-30%), beta glucan (5-10%), the honey powder 1-5%, mix adding 0.5g-5.0 extract in the instant powder by 100g, mix, the packed method according to routine is packaged to be instant powder again.
The theabrownin that above-mentioned steps is obtained adds among maltodextrin: 10-15%, xylitol: 25-40%, antierythrite: 20-35%, soluble pectin: 4-8%, the honey powder 1-5%, press and add the 0.5g-5.0 extract in the instant powder of 100g, make instant granular earlier according to the method for granulating of routine again, packing promptly obtains the instant granular electuary again.
Example 2: the application in health products
The method that obtains of theabrownin is with example 1.
Reducing blood lipid capsule configuration proportion is: the theabrownin extract with hypolipidemic activity: 10-80%, maltodextrin: 5-40%, xylitol: 5-20%, isomalt: 5-15%, polydextrose: 5-15%.
After the material of mentioned component fully mixed, capsule fill method processing routinely promptly obtained having the capsule of regulating the blood lipid metabolism effect.
Claims (2)
1. theabrownin of Pu'er tea with hypolipidemic activity is characterized in that this theabrownin of Pu'er tea obtains through following method:
A. Pu'er cooked tea being crushed to 40 orders, is the ratio throwing tea of 1g/50ml in tealeaves/edible alcohol, and room temperature is placed 6h, and constantly stirs, and tea grounds is collected in 300 order filter cloth centrifugal filtrations;
B. in tea grounds/deionized water the ratio input of 1g/100ml, 25 ℃ of water temperatures, insulation 12h with 300 order filter cloth centrifugal filtrations, collects filtrate; Be the ratio input of 1g/100ml again in tea grounds/deionized water, 25 ℃ of water temperatures, insulation 12h with 300 order filter cloth centrifugal filtrations, merges twice filtrate;
C. be 1: 4 with merging filtrate by filtrate/edible alcohol, unit is that the ratio of v/v is mixed and stirring 10min, leaves standstill 5h, centrifugal collecting precipitate;
D. sediment adds 80% edible alcohol water solution in the ratio of 1g/50ml, and alcohol/water v/v stirs 35 ℃ of insulation 3h behind the 10min, centrifugal collection purifying sediment;
E. with the edible alcohol water solution of purifying sediment in the ratio adding 40% of 1g/100ml, alcohol/water v/v, 35 ℃ of insulation 3h behind the stirring 20min, brownish red filtrate is collected in centrifugal filtration, remaining sediment repeats the edible alcohol water solution in the ratio adding 40% of 1g/100ml again, alcohol/water v/v, 35 ℃ of insulation 3h behind the stirring 20min, centrifugal filtration, collect brownish red filtrate, merge filtrate twice;
F. the filtrate that e step is obtained is after the vacuum decompression of routine concentrates, and vacuum drying promptly obtains having the theabrownin of hypolipidemic activity.
2. the said theabrownin of Pu'er tea with hypolipidemic activity of claim 1 is characterized in that the application of this theabrownin of Pu'er tea as food and products and health products additive agent.
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| CN102258099B (en) * | 2011-05-27 | 2013-02-06 | 云南农业大学 | Camellia sinensisvar assamica Pu'er tea theabrownin and application thereof |
| CN103504048A (en) * | 2012-06-29 | 2014-01-15 | 云南天士力帝泊洱生物茶集团有限公司 | High, medium and low-poly theabrownin and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1742594A (en) * | 2005-06-24 | 2006-03-08 | 云南农业大学 | Pu'er tea and use thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1742594A (en) * | 2005-06-24 | 2006-03-08 | 云南农业大学 | Pu'er tea and use thereof |
Non-Patent Citations (3)
| Title |
|---|
| 周斌星，等.普洱茶茶色素的研究进展.《茶叶科学技术》.2005,(第3期),8-9. * |
| 李连喜.不同制法普洱茶茶褐素及其在贮存中变化的研究.《西南大学硕士学位论文》.2005,1-35. * |
| 樊蓉.普洱茶多酚氧化产物的提取分离、成分分析及活性评价.《华中农业大学硕士学位论文》.2007,1-40. * |
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