KR20120001037A - The manufacturing method of korean hot pepper paste using gastrodia elata blume and the product - Google Patents

The manufacturing method of korean hot pepper paste using gastrodia elata blume and the product Download PDF

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KR20120001037A
KR20120001037A KR1020100061609A KR20100061609A KR20120001037A KR 20120001037 A KR20120001037 A KR 20120001037A KR 1020100061609 A KR1020100061609 A KR 1020100061609A KR 20100061609 A KR20100061609 A KR 20100061609A KR 20120001037 A KR20120001037 A KR 20120001037A
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cheonma
powder
glutinous rice
syrup
red pepper
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KR101204028B1 (en
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김귀영
문혜경
이수원
윤세진
이동은
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경상북도 상주시
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/10Natural spices, flavouring agents or condiments; Extracts thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L11/00Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/05Mashed or comminuted pulses or legumes; Products made therefrom
    • A23L11/07Soya beans, e.g. oil-extracted soya bean flakes
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    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L11/00Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/50Fermented pulses or legumes; Fermentation of pulses or legumes based on the addition of microorganisms
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    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
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    • A23L19/09Mashed or comminuted products, e.g. pulp, purée, sauce, or products made therefrom, e.g. snacks
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/10Natural spices, flavouring agents or condiments; Extracts thereof
    • A23L27/14Dried spices
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/40Table salts; Dietetic salt substitutes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/40Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by drying or kilning; Subsequent reconstitution
    • A23L3/44Freeze-drying
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/51Concentration
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    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10Cereal-derived products
    • A23L7/104Fermentation of farinaceous cereal or cereal material; Addition of enzymes or microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/20Malt products
    • A23L7/25Fermentation of cereal malt or of cereal by malting
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    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00Food ingredients
    • A23V2250/15Inorganic Compounds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00Food ingredients
    • A23V2250/20Natural extracts
    • A23V2250/21Plant extracts
    • A23V2250/2132Other phenolic compounds, polyphenols
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    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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Abstract

PURPOSE: Red pepper paste containing gastrodia elata, and a producing method thereof are provided to offer the functionality of the gastrodia elata by using freeze-dried gastrodia elata powder. CONSTITUTION: A producing method of red pepper paste containing gastrodia elata comprises the following steps: soaking glutinous rice in water after washing, and grinding the glutinous rice to obtain powder; mixing the glutinous rice powder with malt water for saccharifying; filtering and boiling the obtained liquid, and cooling to obtain glutinous rice syrup; inserting freeze-dried gastrodia elata powder into the malt water for saccharifying, and filtering and boiling the obtained liquid before cooling to obtain gastrodia elata syrup; mixing the glutinous rice syrup and the gastrodia elata syrup; and adding red pepper powder, fermented soybean powder, and salt into the syrup mixture to obtain the red pepper paste.

Description

천마고추장의 제조방법 및 그 고추장{The manufacturing method of Korean hot pepper paste using Gastrodia elata Blume and the product}The manufacturing method of Korean hot pepper paste using Gastrodia elata Blume and the product}

본 발명은 천마의 기능성을 살리면서도 적절한 맛과 향을 가져 양념으로서 적절하게 섭취될 수 있도록 한 천마고추장의 제조방법 및 그 고추장에 관한 것이다.
The present invention relates to a manufacturing method of cheonma pepper and the red pepper paste so that it can be properly ingested as a seasoning while taking advantage of the functionality of cheonma.

천마(Gastrodia elata Blume)는 난초목 난초과의 여러해살이풀로 뿌리는 없고 감자와 같은 괴경으로 되어있는 다년초로서, 줄기는 붉은 밤색이고 계란모양의 비늘잎이 드물게 붙어있으며, 줄기의 윗부분은 천마의 종류에 따라 노란색과 빨간색으로 꽃이 핀다. 표면은 윤상(輪狀)의 비늘조각이 늘어선 형태로 원기둥꼴로 곧추서며 황적색을 띤다. 보통 잎은 없고 비늘 조각 잎이 성기게 나며 하부의 것은 짧은 잎집을 형성한다. 지상부에 형성된 줄기의 색깔에 따라서 홍천마 및 청천마(Gastrodia elata) 그리고 녹천마(Gastrodia gracilis)로 구분되어지고, 괴경과 줄기는 약용으로 이용되고 있다.Gastrodia elata Blume is a perennial herb of the orchid family Orchidaceae. It is a perennial herb that is not rooted and has a potato-like tuber. The stem is reddish brown and rarely has egg-shaped scaly leaves. Depending on it blooms in yellow and red. The surface is yellowish red with cylindrical shape in the form of scales of round shape. Usually there is no leaf, scaly leaves are sparse, and the lower one forms short leaflets. According to the color of the stem formed on the ground part, it is divided into red and green horses (Gastrodia elata) and green horse (Gastrodia gracilis). Tubers and stems are used for medicinal purposes.

한방에서 천마는 뇌신경쇠약증(brain nervous breakdown), 진통(pain), 두통(headache), 중풍(paralysis), 고혈압(hypertension) 등에 효과를 보이는 약재로 알려져 있는데, 신경을 튼튼하게 하며 피를 보하고 머리를 검게 한다는 말이 전해져 오고 있다. 또한 콩팥염(kidney inflammation), 당뇨병(glycosuria) 등에도 사용된다.Chemo horses are known for their effects on brain nervous breakdown, pain, headache, headache, paralysis, hypertension, etc. Black has been said to have been. It is also used in children's inflammation, diabetes and glycosuria.

천마에 함유되어 있는 성분은 대부분 페놀성화합물로서 gastrodin, 페놀성 배당체, 유황 함유 페놀성 화합물, 유기산, 당 및 β-sitosterol 등이 분리되어 보고되었으며, 그 외에 sterol류, cholesterol, p-hydroxylbenzyl alcohol, p-hydroxybenzaldehyde와 vanillin 등의 성분에 대해서도 알려져 있다.
Most of the components contained in Cheonma are phenolic compounds, and gastrodin, phenolic glycosides, sulfur-containing phenolic compounds, organic acids, sugars and β-sitosterol have been reported separately. In addition, sterols, cholesterol, p-hydroxylbenzyl alcohol, Ingredients such as p-hydroxybenzaldehyde and vanillin are also known.

현재까지의 천마에 대한 연구는 천마의 무기성분 및 항산화 작용에 관한 연구, 천마의 일반성분과 기능성 조사, 추출용매에 따른 천마 농축액의 리올로지 특성, 건조방법에 따른 천마의 성분분석, 천마의 식품학적 성분분석 및 건조방법에 따른 특성 변화, 청마의 휘발성 향기 성분, 천마의 항혈소판 및 항혈전활성에 대한 연구, 천마추출물이 관상 순환기에 미치는 영향, 천마분말을 첨가한 식빵의 품질특성 등이 보고되었으나 천마를 가공식품에 적용하려는 연구는 미비한 실정이다. 또한 천마는 다양한 효능에도 불구하고 독특한 맛과 냄새로 인하여 식품으로의 이용성이 제한되고 있다.To date, the research on the chemistry of inorganic horses and antioxidants, the research on the general components and functionalities of the horses, the rheological properties of the concentrates by the extraction solvents, the analysis of the ingredients of the horses by the drying method, and the foods of the horses Analysis of chemical composition and characteristics of drying method, volatile aroma component of cheongma, research on antiplatelet and antithrombotic activity of cheonma, effect of cheonma extract on coronary circulation, quality characteristics of bread with cheonma powder However, the research to apply the cheonma to processed food is insufficient. In addition, despite the various effects, the cheonma has limited use as a food due to its unique taste and smell.

최근 소비자들의 건강정보에 대한 관심 고조로 인해 저열량식 제품 및 건강 기능성 소재를 함유한 제품에 대한 구매의사가 높아지고 있으며, 국민의 소득이 높아지고 식생활의 서구화로 고혈압이나 콜레스테롤 수치가 높아서 발생하는 뇌출혈, 고지혈증 등의 여러 가지 성인병들의 문제가 크게 대두되고 있다. 또한 FTA 등 농산물 시장 개방에 따른 농업생산 및 농가소득의 불안요소가 점점 커지고 있는 현실로 개방화에 맞서 경쟁력 있는 안정적인 소득원의 기반 구축이 절실히 필요한 실정이다. 이에 지역의 생물자원을 활용한 지역특산제품의 고품질화를 유도하여 상품의 차별화를 유도함으로서 지역특산품의 비교우위를 점할 수 있는 기반을 구축할 필요가 있다.
Recently, due to the increasing interest in health information of consumers, purchase intentions for low calorie-type products and products containing health functional materials are increasing, and cerebral hemorrhage and hyperlipidemia caused by high blood pressure and cholesterol level due to high income of people and westernization of diet The problem of various adult diseases such as the back is emerging. In addition, as the unstable factor of agricultural production and farm income is increasing due to the opening of agricultural market such as FTA, it is urgently needed to establish a stable and stable source of income against openness. Therefore, it is necessary to establish a foundation to gain a comparative advantage of regional specialty products by inducing high quality of local specialty products using local biological resources to induce differentiation of products.

본 발명은 상기와 같은 배경하에서 안출된 것으로서, 본 발명의 목적은 여러 가지 기능을 가진 천마를 우리나라 전통식품인 고추장에 도입하는 데 있다.The present invention has been made under the above-mentioned background, an object of the present invention is to introduce the cheonma with various functions into Korean traditional gochujang.

본 발명은 또한 위와 같은 시도를 통해 천마의 활용성을 극대화하고 지역특산물의 활용도를 배가하는 데 있다.
The present invention also aims to maximize the utilization of Cheonma and doubling the utilization of local specialties through the above attempts.

상기와 같은 본 발명의 천마고추장 제조방법은, 진공동결 건조한 천마분말로 만든 천마물엿 성분을 이용하여 제조하는 것을 특징으로 한다.The manufacturing method of cheonma pepper as described in the present invention as described above is characterized in that it is prepared by using the cheonma syrup made from cheonma powder vacuum dried.

더 구체적으로 본 발명의 제조방법은,More specifically, the manufacturing method of the present invention,

(1) 찹쌀을 세척하여 물에 불린 다음 갈아서 분말화한 다음, 상기 분쇄한 찹쌀분말을 엿기름액에 넣어 당화하고 그 여과한 액체를 40~50°Brix가 될 때까지 끓여서 농축하고 냉각시켜 찹쌀물엿을 제조하는 단계; (2) 상기 (1) 단계와는 별도로, 진공동결건조한 천마분말을 엿기름액에 넣어 당화하고 그 여과한 액체를 40~50°Brix가 될 때까지 끓여서 농축하고 냉각시켜 천마물엿을 제조하는 단계; 그리고 (3) 상기 찹쌀물엿과 천마물엿을 섞고 다시 거기에 고춧가루, 메줏가루 및 소금을 넣어 고추장을 제조하는 단계;로 이루어지는 것을 특징으로 한다.(1) Wash the glutinous rice, soak it in water, grind it, and powder it. Put the crushed glutinous rice powder into malt liquid, saccharify it, boil the filtered liquid until 40 ~ 50 ° Brix, concentrate it, and cool it. Preparing a; (2) separately from the step (1), the step of vacuum freeze dried Cheonma powder into malt liquid and saccharified and boiled the filtered liquid until 40 ~ 50 ° Brix to concentrate and cool to prepare the Cheonma syrup; And (3) mixing the glutinous rice syrup and cheonma syrup and adding red pepper powder, buckwheat flour and salt therein to prepare red pepper paste.

이때, 상기 각 단계들을 통해 투입되는 찹쌀:천마분말:고춧가루:메줏가루:소금의 중량비는 5.4 : 0.5~0.7 : 5.9~6.1 : 2.4~2.6 : 1.9~2.1 인 것을 특징으로 한다.At this time, the weight ratio of the glutinous rice: cheonma powder: red pepper powder: buckwheat powder: salt added through each of the steps is 5.4: 0.5 to 0.7: 5.9 to 6.1: 2.4 to 2.6: 1.9 to 2.1.

더욱 바람직하게 찹쌀:천마분말:고춧가루:메줏가루:소금의 중량비는 5.4 : 0.6 : 6.0 : 2.5 : 2.0 이다.More preferably, the weight ratio of glutinous rice: Cheonma powder: red pepper powder: mesen powder: salt is 5.4: 0.6: 6.0: 2.5: 2.0.

본 발명은 또한 진공동결 건조한 천마분말로 만든 천마물엿 성분을 이용하여 제조되는 천마고추장인 것을 특징으로 한다.The present invention is also characterized in that the cheonma pepper paste prepared by using the cheonma syrup component made from dried vacuum freeze cheonma powder.

본 발명은 또한 전술한 제조방법 중 어느 하나의 방법에 의하여 제조되는 제조되는 천마고추장인 것을 특징으로 한다.
The present invention is also characterized in that the cheonma pepper is manufactured by any one of the above-described manufacturing method.

본 발명에 따르면, 여러 가지 바람직한 기능을 가진 천마를 우리나라 전통식품인 고추장에 도입하여 기능성 식품화하는 효과를 거둘 수 있다.According to the present invention, it is possible to achieve the effect of functionalized food by introducing cheonma with various desirable functions to the traditional Korean gochujang.

또한 이를 통해 천마의 활용성을 극대화하고 지역 재배농가의 소득을 증대할 수 있다.
This will also maximize the utilization of the cheonma and increase the income of local farmers.

도 1은 전통 고추장의 제조방법을 나타낸 것이다.Figure 1 shows a method of manufacturing a traditional gochujang.

이하 본 발명을 실시예와 함께 상세히 설명한다.
Hereinafter, the present invention will be described in detail with examples.

1. 전통고추장과 천마고추장의 제조1. Manufacture of Traditional and Red Pepper Paste

일반적으로 고추장을 담그려면 고춧가루와 메줏가루, 엿기름가루, 소금 등이 필요하다. 찹쌀을 깨끗이 씻어 불린 다음 가루로 만들고 엿기름을 우린 물을 넣어 삭힌 다음 끓여서 찌꺼기를 짜내고, 뭉근한 불에서 끓여 묽은 조청을 곤 다음 식힌다. 메줏가루와 고춧가루를 넣어 고루 섞고, 찹쌀 조청을 넣어 농도를 맞춘다. 하루쯤 지난 뒤 소금을 넣어 간을 맞춘다. 모든 재료가 섞어지고 간이 알맞게 되면 소독된 항아리에 담아 두고 햇볕이 좋은 날 뚜껑을 열어 볕을 쪼이면서 숙성시킨다.
Generally, red pepper paste, red pepper powder, malt powder, malt powder, and salt are needed to make red pepper paste. Wash the glutinous rice, soak it, make it into powder, and grind the malt with water, then boil it and squeeze it out, boil it in a coarse fire, wash it with diluted rice, and then cool it. Add buckwheat flour and red pepper powder and mix well. Add glutinous rice syrup to adjust the concentration. After a day or so, add salt and season it. Once all the ingredients are mixed and the liver is in the right place, put them in a disinfected jar and let them ripen by opening the lid on a sunny day.

발명자들은 전통고추장과 배합을 달리하는 천마고추장을 만들어 시험을 하였다.The inventors made and tested Cheonma red pepper paste which is different from the traditional red pepper paste.

먼저 전통고추장은 도 1에 나타난 바와 같이 제조하였다. 찹쌀 6kg을 씻어서 24시간 동안 물에 불린 다음 갈아서 분말화하였다. 그리고 이와 별도로 엿기름 2kg에 물 15L를 가하여 6시간 동안 침지시킨 후 엿기름액을 추출하였다. 그다음, 이 엿기름액에 분쇄해둔 찹쌀가루를 넣어 60℃에서 5시간 동안 당화하여 여과한 후 50°Brix가 될 때까지 끓여서 농축한 다음 냉각시켰다. 이렇게 얻어진 것이 찹쌀물엿(=찹쌀조청)이다. 이 찹쌀물엿에, 고춧가루 6kg, 메줏가루 2.5kg, 소금 2kg을 넣어 전통고추장을 제조하였다(표 1의 control).First, traditional pepper paste was prepared as shown in FIG. 6kg of glutinous rice was washed, soaked in water for 24 hours, and then ground and powdered. Separately, 15L of water was added to 2kg of malt, and after dipping for 6 hours, malt solution was extracted. Then, put the glutinous rice powder pulverized in the malt solution, saccharified and filtered for 5 hours at 60 ℃, boiled to 50 ° Brix, concentrated and cooled. Thus obtained glutinous rice syrup (= glutinous rice syrup). To the glutinous rice syrup, 6 kg of red pepper powder, 2.5 kg of buckwheat flour, and 2 kg of salt were added to prepare a traditional red pepper paste (control of Table 1).

또한 찹쌀을 5.7kg 사용하여 위와 같은 방법으로 찹쌀물엿을 만들고, 0.3kg의 진공동결건조한 천마분말을 사용하여 천마물엿(=천마조청)을 만든 다음(천마물엿을 만드는 과정은 찹쌀물엿을 만드는 과정과 동일, 엿기름과 물의 경우에도 같은 비율로 환산하여 사용), 찹쌀물엿과 천마물엿을 섞고 다시 거기에 고춧가루 6kg, 메줏가루 2.5kg, 소금 2kg을 넣어 천마고추장을 제조하였다(표 1의 GB 5%). GB 10%, GB 15%, GB 20%의 경우에도 사용한 찹쌀과 천마의 양을 제외하고는 동일하다(표 1 참조).In addition, 5.7kg of glutinous rice is used to make glutinous rice syrup in the same way as above, and 0.3kg of vacuum-dried cheonma powder is used to make cheonma syrup (= cheonmazocheong) and then the process of making cheonma syrup is In the case of malt and water, the same ratio was used), glutinous rice syrup and Cheonma syrup were mixed, and 6 kg of red pepper powder, 2.5 kg of buckwheat flour, and 2 kg of salt were added thereto to prepare Cheonma pepper paste (GB 5% in Table 1). . The same applies to GB 10%, GB 15%, and GB 20%, except for the amount of glutinous rice and cheonma used (see Table 1).

GBP 10%의 경우에는, GB 10%의 경우와 다른 것은 같고 다만 천마를 천마물엿이 아닌 분말 상태로 투여하는 방법으로 하였다(표 1 참조).
In the case of GBP 10%, the same method as that in GB 10% was the same, except that Cheon horse was administered in a powder state instead of starch syrup (see Table 1).

Figure pat00001

Figure pat00001

2. 실험 방법2. Experimental method

1) 일반성분 분석1) General Ingredient Analysis

일반성분은 AOAC 방법에 준하여 분석하였다. 즉, 수분함량은 105℃ 상압가열건조법, 조단백질은 Kjeldahl 질소정량법, 조지방은 Soxhlet 추출법, 조회분은 직접회화법, 조섬유는 Fibertec으로 측정하여 백분율로 나타내었다. 가용성 무질소물은 100에서 수분, 조단백질, 조지방, 조회분 및 조섬유를 제외한 값으로 구하였다.
General components were analyzed according to the AOAC method. In other words, the moisture content was 105 ℃ atmospheric pressure drying method, crude protein was measured by Kjeldahl nitrogen determination method, crude fat was measured by Soxhlet extraction method, crude ash was measured by direct ashing method, and crude fiber was measured by fibertec. Soluble free nitrogen was obtained at 100 excluding moisture, crude protein, crude fat, crude ash and crude fiber.

2) pH 측정2) pH measurement

천마를 첨가한 고추장의 pH 측정은 시료를 5g 정확하게 칭량하여 3차 증류수 50mL에 희석하여 3시간 침출시킨 후 Whatman No. 5로 여과하여 여과액을 이용하여 pH meter(691 pH Meter, Metrohm, Swiss)를 사용하여 측정하였다.
The pH measurement of kochujang added with cheonma was measured by accurately weighing 5 g of the sample, diluted in 50 mL of tertiary distilled water, and leached for 3 hours. Filtration was carried out by 5 and measured using a pH meter (691 pH Meter, Metrohm, Swiss) using the filtrate.

3) 염도 측정 3) Salinity Measurement

천마를 첨가한 고추장의 염도 측정은 시료를 5g 정확하게 칭량하여 3차 증류수 50mL에 희석하여 3시간 침출시킨 후 Whatman No. 5로 여과하여 여과액을 이용하여 salt-meter(Atago ES-421, Atago Co., Japan)를 사용하여 3회 반복 측정한 값을 희석배수를 곱하여 평균값으로 나타내었다.
Salinity measurement of kochujang added with Cheonma was weighed 5g accurately, diluted in 50mL of tertiary distilled water, and leached for 3 hours. Filtration at 5 was repeated three times using a salt-meter (Atago ES-421, Atago Co., Japan) using the filtrate to represent the average value by multiplying the dilution factor.

4) 색도 측정 4) Chromaticity Measurement

천마를 첨가한 고추장의 색도 측정은 색차계(Spectrocolorimeter, USXE/ SAV/UV-2, Hunterlab Overseas, Ltd, U.S.A)를 이용하여 명도(L-value, lightness), 적색도(a-value, redness) 및 황색도(b-value, yellowness) 값을 3회 반복 측정하여 평균값으로 나타내었다. 이때의 표준 백색판(L=99.11, a=0.23, b=-0.28)을 사용하였다.
The chromaticity measurement of kochujang added with cheonma was measured by using a colorimeter (Spectrocolorimeter, USXE / SAV / UV-2, Hunterlab Overseas, Ltd, USA) for lightness (L-value, lightness) and redness (a-value, redness). And yellowness (b-value, yellowness) value was measured three times and expressed as an average value. At this time, a standard white plate (L = 99.11, a = 0.23, b = -0.28) was used.

5) 총페놀 함량 측정 5) Determination of total phenolic content

총 페놀 함량은 페놀성물질이 phosphomolybdic acid와 반응하여 청색을 나타내는 것을 이용한 Folin-Denis법으로 측정하였다. 즉, 시료 5g에 80% 에탄올용액 100 mL를 가하여 환류냉각기가 부착된 heating mantel에서 80℃, 2시간 반복추출 후 Whatman No. 5로 여과하였다. 여과액은 hexane으로 지질을 제거한 다음 40℃ 진공농축 건고 후 80% 에탄올용액 5 mL로 정용 하였다. 위의 정용액 1 mL 와 Folin-Denis시약 3 mL를 혼합하여 30분간 실온에 방치한 다음 10% Na2CO3 용액 3 mL를 가하여 혼합하고 실온에서 1시간 정치시킨 후 760 nm에서 흡광도를 측정하였다. 이때 표준검량곡선은 garlic acid를 이용하여 작성하였다.
Total phenolic content was measured by Folin-Denis method using phenolic substances reacting with phosphomolybdic acid to show blue color. In other words, 100 mL of 80% ethanol solution was added to 5 g of the sample, followed by repeated extraction at 80 ° C. for 2 hours in a heating mantel equipped with a reflux condenser. It was filtered by 5. The filtrate was removed with lipids in hexane and then dried under vacuum at 40 ° C., and then used as 5 mL of 80% ethanol solution. 1 mL of the above solution and 3 mL of Folin-Denis reagent are mixed and allowed to stand at room temperature for 30 minutes, followed by a 10% Na 2 CO 3 solution. 3 mL was added, mixed, and left standing at room temperature for 1 hour, followed by measurement of absorbance at 760 nm. The standard calibration curve was prepared using garlic acid.

6) DPPH radical 소거활성 측정6) Measurement of DPPH radical scavenging activity

DPPH(α,α-diphenyl-β-picrylhydrazyl) radical 소거활성은 Blois의 방법에 준하여 변형하여 측정하였다. 각 추출물 1 mL에 60 μM DPPH 3 mL를 넣고 vortex한 후 15분 동안 암소에 방치한 다음 517 nm에서 흡광도를 측정하여 다음 식에 의하여 나타내었다.
DPPH (α, α-diphenyl-β-picrylhydrazyl) radical scavenging activity was measured by modification according to the method of Blois. 3 mL of 60 μM DPPH was added to 1 mL of each extract, vortexed, and left in the dark for 15 minutes. The absorbance at 517 nm was measured and expressed by the following equation.

Figure pat00002

Figure pat00002

7) 유기산 함량 측정7) Determination of Organic Acid Content

유기산 분석은 시료 5 g에 80% 에탄올용액 100 mL를 가하여 환류냉각기가 부착된 heating mantle에서 80℃, 2시간 반복추출 후 Watman No. 5로 여과하였다. 여과액은 hexane으로 지질을 제거하고 40℃ 진공 농축 건고 후 증류수 5 mL로 정용한 다음 고분자 물질과 색소를 제거하기 위하여 Sepak C18 cartridge 및 0.2 μm membrane filter로 여과한 후 HPLC(Waters 2695, Waters Co., USA)로 분석하였다. 이때 column은 YMC-pack ODS-AQ(YMC Co. 4.6 × 250 mm)를 사용하였으며, column 온도는 상온에서 분석하였고, mobile phase은 100mM phosphate buffer, flow rate은 0.7 mL/min., 검출기는 photodiode array(PDA) detector, Waters 2996, Waters Co., USA)로 분석하였다. 표준품은 oxalic acid, citric acid, tartaric acid, malic acid, acetic acid, succinic acid 및 lactic acid(Sigma, U.S.A.)를 일정량씩 혼합하여 증류수에 녹여 표준용액으로 사용하였다. 표준품과 시료의 유기산 성분은 머무른 시간(tR)을 직접 비교하여 확인하였고 각 표준품의 검량곡선을 작성하여 peak의 면적으로 개별 유기산성분의 함량을 산출하였다.
For organic acid analysis, 100 ml of 80% ethanol solution was added to 5 g of the sample, followed by repeated extraction at 80 ° C. for 2 hours in a heating mantle equipped with a reflux condenser. It was filtered by 5. The filtrate was removed by hexane, concentrated in vacuo at 40 ° C., dried in 5 mL of distilled water, filtered through Sepak C 18 cartridge and 0.2 μm membrane filter to remove polymers and pigments, followed by HPLC (Waters 2695, Waters Co. , USA). The column was YMC-pack ODS-AQ (YMC Co. 4.6 × 250 mm), column temperature was analyzed at room temperature, mobile phase is 100mM phosphate buffer, flow rate is 0.7 mL / min., Detector is photodiode array (PDA) detector, Waters 2996, Waters Co., USA). As standard, oxalic acid, citric acid, tartaric acid, malic acid, acetic acid, succinic acid, and lactic acid (Sigma, USA) were mixed in distilled water and used as standard solutions. The organic acid components of the standard and the sample were confirmed by directly comparing the retention time (t R ), and the calibration curve of each standard was prepared to calculate the content of the individual organic acid components in the area of the peak.

Figure pat00003

Figure pat00003

8) 유리 아미노산 함량 측정8) Determination of Free Amino Acid Content

유리 아미노산을 분석하기 위해 시료를 약 1g씩 정확히 칭량하여 삼각플라스크에 넣고 80% ETOH 용액을 100ml 가하여 약 24시간 진탕추출하고, 그 추출물을 감압여과하여, 45℃ Water bath에서 감압농축한 후 0.2M lithium citrate buffer 용액(pH 2.2) 5ml로 정용하고, Sepak C18처리한 후 0.45um membrane filter로 재여과하여 Automatic amino acid analyzer(Biochrom-30, Pharacia Biotech Co., Swiss)로 분석하였다. 이때 column은 Li form column으로 분석하였다.
In order to analyze free amino acids, weigh 1 g of the sample accurately and place it in an Erlenmeyer flask, add 100 ml of 80% ETOH solution, shake extract for about 24 hours, filter the extract under reduced pressure, and concentrate under reduced pressure in 45 ℃ water bath, and then 0.2M. 5 ml of lithium citrate buffer solution (pH 2.2) was applied, Sepak C 18 treated and re-filtered with 0.45um membrane filter and analyzed by Automatic amino acid analyzer (Biochrom-30, Pharacia Biotech Co., Swiss). At this time, the column was analyzed as a Li form column.

Figure pat00004

Figure pat00004

9) 무기질 함량 측정9) Measurement of mineral content

시료 10g을 삼각플라스크에 칭량하여 질산을 가한 후 뚜겅을 덮는다. 급격한 반응이 일어나지 않도록 실온에서 12시간 이상 방치 후 100℃에서 24시간 이상을 가열하여 노란색의 맑은 용액이 될 때까지 실시하고 이때 급격한 반응이 일어나 끓으면 즉시 열판에서 내려놓는다. 반응이 끝나면 삼각플라스크에서 뚜껑을 열고 산을 증발시킨 후 다시 질산을 넣고 산이 완전히 증발할 때까지 재반응시켜 유기질을 제거한다. 재반응 후 열판에서 분리하여 0.2N 질산용액을 20㎖ 가하여 24시간 재용출 시킨 시료 용액을 0.45um membrane filter로 여과하여 50 mL volumetric flask로 정용한 후 분석용액으로 하였다. Ca, Co, Cu, K, Mg, Mo, Na, Zn 등은 ICP(Inductively Coupled Plasma, IRis Intrepid, Thermo Elemental Co., UK)로 A393.366(85), A228.616(147), A324.754(103), A766.491(44), A285.213(117), A588.995(57), A213.856(157)에서 각각 분석하였다. 분석조건은 approximate RF power가 1,150w이며, analysis pump rate는 100rpm, nebulizer pressure와 observation height는 각각 20psi 및 15mm로 하였다.
Weigh 10 g of the sample into a Erlenmeyer flask, add nitric acid, and cover the lid. In order to prevent a sudden reaction at least for 12 hours at room temperature and heated at 100 ℃ for more than 24 hours until a clear yellow solution. After the reaction, open the lid in the Erlenmeyer flask, evaporate the acid, add nitric acid and re-react until the acid is completely evaporated to remove organic matter. After re-reaction, the sample solution, which was separated from the hotplate, added 20 mL of 0.2N nitric acid solution, and redissolved for 24 hours, was filtered through a 0.45um membrane filter, and then purified by 50 mL volumetric flask to obtain analytical solution. Ca, Co, Cu, K, Mg, Mo, Na, Zn and the like are ICP (Inductively Coupled Plasma, IRis Intrepid, Thermo Elemental Co., UK) A 393.366 (85), A 228.616 (147), A 324.754 (103 ), A 766.491 (44), A 285.213 (117), A 588.995 (57), and A 213.856 (157) , respectively. The analysis conditions were approximate RF power of 1,150w, analysis pump rate of 100rpm, nebulizer pressure and observation height of 20psi and 15mm, respectively.

10) 관능평가10) sensory evaluation

관능평가는 경북대학교 상주캠퍼스 식품영양학과 대학원생 및 4학년 학부생을 대상으로 20명의 훈련된 패널을 대상으로 하여 고추장의 색, 향, 단맛, 쓴맛, 이취, 입안에서의 느낌, 전체적인 기호도 대하여 5점 평점법(1 : 아주 나쁘다, 2 : 나쁘다, 3 : 보통이다, 4 : 좋다, 5 : 아주 좋다)로 평가한 다음 Duncan's multiple range test로 유의성을 검정하였다. 관능평가용 시료는 100일 숙성된 고추장을 채취하여 흰접시에 일정량씩 나누어 담은 시료를 함께 제공하여 관능검사를 실시하였다.
The sensory evaluation was conducted on 20 trained panelists at Kyungpook National University's Sangju Campus, Department of Food Science and Nutrition, and undergraduate students. The score was 5 points for the color, aroma, sweetness, bitterness, malodor, mouthfeel, and overall preference of Kochujang. The method was evaluated by the method (1: very bad, 2: bad, 3: normal, 4: good, 5: very good) and then tested for significance by Duncan's multiple range test. For the sensory evaluation sample, 100 days of aged kochujang was taken and provided with a sample containing a predetermined amount in a white plate, and the sensory test was performed.

3. 실험 결과3. Experimental Results

1) 일반성분1) General Ingredient

천마 고추장 숙성기간 중 일반성분 분석 결과를 표 4에 나타내었다. 수분함량에 있어서는 숙성 0일, 40일, 70일, 100일 모두 각 구간별 함량은 비슷한 값을 나타내고 있으며 유의적인 차이는 없는 것으로 생각된다.Table 4 shows the results of the analysis of the general components during the fermentation of Chunma Kochujang. In the water content, the contents of each section showed similar values for 0, 40, 70 and 100 days of aging, and there is no significant difference.

조단백질의 경우 숙성 0일에서는 낮은 값을 보였으나 숙성 40일 이후부터는 모든 구간이 비슷한 값을 나타내고 있는데 숙성 100일째 GB 5%가 5.08로 가장 낮은 값은 GB 15%가 5.19로 가장 높은 값을 나타내었다.Crude protein showed low value at 0 days of fermentation, but after 40 days of fermentation, all of the sections showed similar values. On the 100th day of fermentation, GB 5% was 5.08 and lowest was GB 15% at 5.19. .

조지방의 분석결과 숙성 0일에서는 높은 값을 나타내었으나 숙성기간이 길어질수록 전반적으로 가소 경량을 보여 숙성 100일째에는 GBP 10%가 0.2로 가장 낮은 값을 Control이 0.78로 가장 높은 값을 나타내었다.As a result of the analysis of crude fat, it showed high value at 0 days of fermentation, but the longer the fermentation period, the more plasticized overall. On the 100th day of fermentation, GBP 10% showed the lowest value as 0.2, and Control showed the highest value as 0.78.

조회분 함량에 있어서는 숙성 70일까지는 값이 증가 경향을 보였으나 숙성 100일째에는 GBP 10%가 9.25로 가장 높은 값을 GB 5%가 7.32로 가장 낮은 값을 나타내었다.In terms of ash content, the value tended to increase up to 70 days of fermentation, but at 100 days of fermentation, GBP 10% showed the highest value of 9.25 and GB 5% showed the lowest value of 7.32.

조섬유 함량에 있어서는 숙성기간 동안 큰 차이를 보이지 않고 조금의 차이를 보였으나 이는 시료 채취 및 실험상의 오차 범위로 사료되며 숙성 100일째 GB 5%가 0.82로 가장 높은 값을 GB 20%가 0.26으로 가장 낮은 값을 나타내었다.The crude fiber content did not show a big difference during the ripening period, but showed a slight difference, but this is considered to be a sampling and experimental error range. On the 100th day of fermentation, GB 5% was 0.82, and GB 20% was 0.26, which was the lowest. The value is shown.

탄수화물 함량에 있어서는 숙성 100일째 Control 구간이 40.24로 가장 낮은 값을 보였으며 다른 5구간은 비슷한 값을 나타내었다.In the carbohydrate content, the control section was the lowest as 40.24 at 100 days of aging and the other five sections showed similar values.

열량의 경우 숙성 100일째 Control이 188.58로 가장 낮은 값을 나타내었고 다음으로 GBP 10%가 197.56으로 낮았으며 다른 4구간의 값은 비슷하게 나타났다.
In the case of calories, the control showed the lowest value at 188.58 at 100 days of fermentation, followed by GBP 10% at 197.56.

Figure pat00005

Figure pat00005

2) pH2) pH

천마를 첨가한 고추장의 숙성기간별 pH는 표 5에 나타냈다. pH 측정 결과 전반적으로 숙성초기의 값보다 숙성기간이 길어질수록 pH값이 조금씩 낮아지는 경향을 보였는데 이는 숙성이 되면서 유기산의 함량이 조금씩 증가하는 경향에 따라 pH값이 낮아진 것으로 사료된다.The pH of each kochujang added with cheonma was shown in Table 5. As a result of the pH measurement, the pH value tended to decrease slightly as the ripening period was longer than the initial ripening value. It is thought that the pH value decreases as the organic acid content gradually increases as it matures.

숙성 100일째 pH값은 GBP 10% 4.81으로 가장 높고 다음으로 Control이 4.76으로 높았으며 GB 20%가 4.30으로 가장 낮은 값을 나타내었다.
On the 100th day of fermentation, the pH value was GBP 10% 4.81, followed by Control 4.76, and GB 20% was 4.30, the lowest.

Figure pat00006

Figure pat00006

3) 염도3) salinity

천마를 첨가한 고추장의 숙성기간별 염도는 표 6에 나타내었다. 염도 값의 측정결과 숙성 0일에서 70일까지는 전반적으로 염도가 증가하는 경향을 보이다 숙성 100일째에는 염도값이 조금 낮아지는 경향을 보여주고 있다.The salinity of each kochujang added with cheonma was shown in Table 6. As a result of the measurement of salinity, salinity tends to increase from 0 to 70 days of aging. On the 100th day of aging, salinity tends to be slightly lower.

숙성 100일째 GB 5%구간이 3.30.으로 가장 높은 값을 나타내었고 GB 20%구간이 2.27로 가장 낮은 갓을 나타내었다.
At 100 days of fermentation, GB 5% showed the highest value of 3.30. And GB 20% showed the lowest value of 2.27.

Figure pat00007

Figure pat00007

4) 색도4) Chromaticity

천마를 첨가한 고추장의 숙성기간별 색도는 표 7에 나타내었다.The chromaticity of each kochujang added with cheonma was shown in Table 7.

색도 L값(명도)에 있어서 숙성초기부터 100일째까지 비슷한 값을 나타내고 있으며, 조금의 차이는 시료 채취상의 실험 오차로 사료된다. 숙성 100일째 GB 20%가 40.09로 가장 높은 값을 나타내고 있으며 GBP 10%가 34.19로 가장 낮은 값을 보여 주고 있다.The chromaticity L value (brightness) shows similar values from the beginning of aging to the 100th day, and slight differences are considered to be experimental errors in sampling. On the 100th day of aging, GB 20% showed the highest value of 40.09 and GBP 10% showed the lowest value of 34.19.

a값(적색도)에 있어서는 숙성 40일째 까지는 값이 낮아지는 경향을 보여주다가 70일째 100일째는 값이 높아지는 경향을 보여주고 있으며 70, 100일째 값은 비슷한 값을 보여주고 있다. 100일째 GB 20%가 20.09로 가장 높은 값을 Control이 18.37로 가장 낮은 값을 나타내었다.In the value of a (redness), the value tends to decrease until the 40th day of aging, whereas the value tends to increase in the 70th and 100th days, and similar values are shown in the 70th and 100th days. On the 100th day, GB 20% showed the highest value of 20.09 and Control showed the lowest value of 18.37.

b값(황색도)는 숙성 40일 까지는 값이 낮아지는 경향을 보였으나 숙성기간 40일 이후에는 점차 증가하는 경향을 보여 숙성 100일째 GB 20%가 23.14로 가장 높은 값을 나타내었고 GB 15%가 15.90으로 가장 낮은 값을 나타내었다.
The b value (yellowness) tended to decrease until 40 days of fermentation, but gradually increased after 40 days of fermentation, and GB 20% at 100 days of fermentation showed the highest value at 23.14 and GB 15% The lowest value was 15.90.

Figure pat00008

Figure pat00008

5) 총페놀 함량5) total phenolic content

천마를 첨가한 고추장의 숙성기간별 총페놀 함량은 표 8에 나타내었다. The total phenolic contents of kochujang added with cheonma by aging period are shown in Table 8.

총페놀 함량에 있어서 숙성 40일까지는 증가하는 경향을 보여 주고 있으며, 70일까지는 조금 감소하는 경향을 보여주고 있다. 숙성 100일째에는 70일째 값보다 큰 폭으로 감소하는 경향을 보였는데 GB 20%가 98.54로 가장 낮은 값을 나타내었고 GB 5%가 129.21로 가장 높은 값을 보여 주었다.
The total phenolic content was increased up to 40 days of aging and slightly decreased up to 70 days. On the 100th day of aging, the tendency decreased more than the value on the 70th day. GB 20% showed the lowest value at 98.54 and GB 5% showed the highest value at 129.21.

Figure pat00009

Figure pat00009

6) DPPH radical 소거 활성6) DPPH radical scavenging activity

천마를 첨가한 고추장의 숙성기간별 DPPH radical 소거활성은 표 9에 나타내었다.DPPH radical scavenging activity according to aging period of kochujang added with cheonma was shown in Table 9.

DPPH radical 소거 활성능에서 숙성 40일, 70일 때까지는 값이 증가하는 경향을 보였으나 숙성 70일 이후 100일째 에는 감소하는 경향을 보였다.The value of DPPH radical scavenging activity tended to increase until 40 and 70 days of aging, but decreased after 100 days of aging.

숙성 중 Control은 40일째 93.53, GB 5%는 40일째 93.30, GB 10%는 40일째 93.53, GB15%는 40일째 93.53, GB 20%는 70일째 91.56, GBP 10%는 70일째 94.24로 가장 높은 값을 보였으며 100일째에는 GBP 10%가 80.88로 가장 높은 값을 나타내었으며 GB 15%가 73.55로 가장 낮은 값을 나타내었다.
During fermentation, the highest value was 93.53 at 40 days, GB 5% at 93.30 on 40 days, GB 10% at 93.53 on 40 days, GB15% at 93.53 on 40 days, GB 20% at 91.56 on 70 days, and GBP 10% at 94.24 on 70 days. On day 100, GBP 10% had the highest value of 80.88 and GB 15% had the lowest value of 73.55.

Figure pat00010

Figure pat00010

7) 유기산 함량7) organic acid content

천마 고추장의 숙성기간별 유기산 함량은 표 10에 나타내었다.Organic acid content of cheonma kochujang by aging period is shown in Table 10.

유기산 함량 분석결과 전반적으로 숙성기간이 길어질수록 유기산의 함량은 조금씩 증가하는 경향을 보여주고 있으며, 이러한 유기산의 증가는 고추장의 pH를 낮춰주어 저장기간에 있어서 미생물의 생육을 억제하는 데 영향을 줄 것으로 사료된다.As a result of the analysis of organic acid content, as the ripening period is longer, the content of organic acid tends to increase little by little, and this increase of organic acid will lower the pH of kochujang and affect the growth of microorganisms during the storage period. It is feed.

숙성 100일째 oxalic acid 경우 GBP 10%가 313.10으로 가장 높은 값을 GB 10%가 115.11로 가장 낮은 값을 나타내었으며 lactic acid 경우 GB 15%가 1462.37로 가장 높은 값을 control이 715.38로 가장 낮은 값을 나타내었다.On the 100th day of aging, GBP 10% showed the highest value of 313.10 for oxalic acid and GB 10% showed the lowest value for 115.11, while GB 15% showed the highest value for 1462.37 for lactic acid and control showed the lowest value for 715.38. It was.

천마 고추장에 있어서 유기산 중 lactic acid가 가장 많이 함유되어 있는 것으로 나타났으며 tartaric acid, oxalic acid, malic acid, acetic acid, citric acid, succinic acid가 비슷한 함량으로 함유되어 있는 것으로 나타났다.
It was found that lactic acid was the most abundant in organic acid in cheonma kochujang, and similar contents of tartaric acid, oxalic acid, malic acid, acetic acid, citric acid and succinic acid.

Figure pat00011

Figure pat00011

8) 유리 아미노산 함량8) Free Amino Acid Content

천마 고추장의 숙성기간별 유리 아미노산의 함량 결과는 표 11에 나타내었다.The results of free amino acid content according to aging period of Cheonma Kochujang are shown in Table 11.

천마 고추장에 함유된 유리 아미노산은 약 28종 함유되어 있는 것으로 나타났으며 천마 된장보다는 약 5종이 적은 것으로 나타났다.About 28 kinds of free amino acids contained in Chunma Gochujang appeared to be about 5 kinds less than Chunma Doenjang.

aAba, Car 경우 숙성 40일째 까지는 함유되지 않은 것으로 나타났으나 숙성 70일과 100일째에는 미량 함유 되어 있는 것으로 나타났다.In the case of aAba and Car, they were not contained until 40 days of aging, but they were found to be contained in 70 and 100 days of aging.

Thr, Ser의 경우는 숙성 40일때 까지는 함유되어 있는 것으로 나타났으나 숙성 70일 이후에는 함유되어 있지 않은 것으로 나타났다.Thr and Ser were found to be contained until the age of 40, but not after 70 days of aging.

Aaiba의 경우 Coltrol, GBP 10% 구간에서만 숙성 70일, 100일째 미량 함유 되어 있는 것으로 나타났으며, 1-Mhis의 경우는 Coltrol 경우 숙성 초기부터 100일째에도 전혀 함유되어 있지 않았고, GBP 10%구간 경우 숙성 0일째에는 미량 함유되어 있었으나 숙성이 진행됨으로서 함유되지 않은 것으로 나타났으며, GB 5%, GB 10%, GB 15%, GB 20% 구간의 경우는 숙성 40일째 까지는 함유되어 있지 않은 것으로 나타났으나 숙성 70일째부터 미량 함유되어 있는 것으로 나타났다. 천마고추장에 유리아미노산 중 pro가 가장 많이 함유되어 있었으며, 다음으로 ASn이며, Ala, Val, Glu, Ile, Leu, Phe,가 비슷하게 미량 함유되어 있는 것으로 나타났다.
In the case of Aaiba, only 70% and 100 days of fermentation were contained only in the 10% of the Coltrol and GBP, while 1-Mhis contained no traces even at the 100th day of the fermentation. On the 0th day of fermentation, it was found to be a small amount, but it was not contained as the fermentation progressed.In the case of GB 5%, GB 10%, GB 15%, GB 20%, it was not found until 40 days of aging. It was found to contain a trace amount from the 70th day of aging. The highest amount of pro amino acid in Cheonma pepper paste contains pro, followed by ASn and similar amounts of Ala, Val, Glu, Ile, Leu, Phe.

Figure pat00012

Figure pat00012

Figure pat00013

Figure pat00013

Figure pat00014

Figure pat00014

Figure pat00015

Figure pat00015

9) 무기질 함량9) mineral content

천마고추장의 숙성기간별 무기질 함량 결과는 표 12에 나타내었다.The mineral content of cheonma pepper paste by aging period is shown in Table 12.

천마고추장의 무기질 함량은 Na>K>Mg>Ca의 순으로 함유되어 있는 것으로 나타났으며, Co, Cu, Mo의 경우는 미량 함유되어 있는 것으로 나타났다. Ca의 경우 control이 357.436으로 가장 높은 값을 나타냈으며, Fe는 GB 20%가 가장 높은 것으로 나타났다.The mineral content of Chunma Gochujang was found in the order of Na> K> Mg> Ca, and in the case of Co, Cu, and Mo, it was found to contain a small amount. In the case of Ca, the control showed the highest value with 357.436, and Fe showed the highest with 20% of GB.

K 함유량에서는 GB 5%가 4766.68, Mg, Zn의 경우 control이 각각 567.578, 8.677로 가장 많이 함유하고 있는 것으로 나타났다.For K content, GB 5% contained 4766.68, Mg and Zn, 567.578 and 8.677, respectively.

Na 함량에 있어서 숙성기간별 분석을 보면 data가 hunting이 많아 data가 안정적이지 못한데 이는 배합과정에서의 mixing과 숙성 중 정기적 mixing이 제대로 이루어지지 않아 유기적인 화합과 삼투가 완전하게 이루어지지 않은 것으로 사료되며, 이는 제조공정 중이나 숙성공정 중에서 특별히 관리해야 할 포인트로 사료된다.
In Na content analysis, the data was not stable due to the large amount of hunting. It is considered that the organic mixing and osmosis were not completed because the mixing in the mixing process and the regular mixing during the ripening were not performed properly. This is considered to be a special point to be managed during the manufacturing process or during the aging process.

Figure pat00016

Figure pat00016

10) 관능평가10) sensory evaluation

전통방법으로 제조한 고추장은 100일 동안 숙성시킨 시료를 이용하여 관능평가를 한 결과는 표 13과 같다.The results of sensory evaluation using kochujang prepared by the traditional method for 100 days are shown in Table 13.

천마고추장의 관능검사 결과, 지금까지 전통고추장에 길들여져 있는 맛의 영향에 의해 천마 첨가 고추장의 관능평가가 전통고추장에 비해 조금 낮은 점수가 나왔다. 전통고추장 control의 경우 쓴맛을 제외하고는 모든 구간에서 가장 좋은 점수를 획득하였는데, 이는 천마가 가지고 있는 고유한 맛과 향에 의해 영향을 받는 것으로 사료된다. 천마 첨가 고추장의 경우 GB 10%와 GBP 10%가 거의 비슷한 점수를 획득하였는데, GB 10%가 조금 더 좋은 반응을 얻었다. 이는 고추장을 담글 때 천마가 가지고 있는 전분을 분해해서 첨가하느냐, 아니면 분말그대로 첨가하느냐하는 차이에서 오는 것이라 사료된다.As a result of sensory test of Cheonma Gochujang, the sensory evaluation of Kochujang added with Cheonma was slightly lower than that of Traditional Kochujang due to the influence of taste that has been domesticated in traditional chili sauce. In the case of traditional red pepper control, the best score was obtained in all sections except bitter taste, which is thought to be influenced by the unique taste and aroma of Cheonma. In the case of cheonma added kochujang, GB 10% and GBP 10% scored almost the same, but GB 10% got a better response. This is thought to be due to the difference between adding starch possessed by Cheonma and adding powder as it is.

관능검사 결과, 10% 이상 첨가되는 구간인 GB 15%, GB 20%는 천마의 향과 맛이 굉장히 강해 일반인들에게 거부감을 주었다. 이상 결과에서, 천마고추장은 전통고추장을 제외하고 전반적으로 가장 높은 점수를 획득한 GB10% 구간을 최종 배합비로 선택을 하였다.
As a result of the sensory test, GB 15% and GB 20%, which were added more than 10%, were very strong in the smell and taste of the cheonma, giving the public a sense of rejection. In the above results, the Cheonma Gochujang was selected as the final blending ratio of the GB10% section, which obtained the highest overall score, except for the traditional pepper paste.

Figure pat00017

Figure pat00017

4. 요약 및 결론4. Summary and Conclusion

수분함량에 있어서 각 구간별 함량은 비슷한 값을 나타내고 있으며 유의적인 차이는 없는 것으로 생각되며, 조 단백질은 숙성 100일 째 GB 5%가 5.08로 가장 낮은 값을 GB 15%가 5.19로 가장 높은 값을 나타내었다. 조지방은 숙성100일 째 GBP 10%가 0.2로 가장 낮은 값을 Control이 0.78로 가장 높은 값을 나타내었고, 조회분은 숙성 100일째 GBP 10%가 9.25로 가장 높은 값을 GB 5%가 7.32로 가장 낮은 값을 나타내었다. 조섬유는 숙성 100일째 GB 5%가 0.82로 가장 높은 값을 GB 20%가 0.26으로 가장 낮은 값을 나타내었다. 탄수화물 함량에 있어서는 숙성 100일째 Control 구간이 40.24로 가장 낮은 값을 보였으며 다른 5구간은 비슷한 값을 나타내었다. 열량의 경우 숙성 100일째 Control이 188.58로 가장 낮은 값을 나타내었고 다음으로 GBP 10%가 197.56으로 낮았으며 다른 4구간의 값은 비슷하게 나타났다.The contents of each section showed similar values in water content and there is no significant difference. The crude protein showed the lowest value of 5.08 at 5.08 and the highest value at 5.19 for GB 5% at 100 days of aging. Indicated. Crude fat showed the lowest value of GBP 10% at 0.2 on the 100th day of fermentation and highest value of Control at 0.78. On the day of fermentation, GBP 10% had the highest value at 9.25 on the 100th day of aging and GB 5% at 7.32. Low values were shown. Crude fiber showed the highest value of 0.82 for GB 5% at 100 days and the lowest value at 0.26 for GB 20%. In the carbohydrate content, the control section was the lowest as 40.24 at 100 days of aging and the other five sections showed similar values. In the case of calories, the control showed the lowest value at 188.58 at 100 days of fermentation, followed by GBP 10% at 197.56.

pH는 숙성이 되면서 유기산의 함량이 조금씩 증가하는 경향에 따라 pH값이 낮아진 것으로 사료되며, 숙성 100일째 GBP 10% 4.76으로 가장 높고 GB 20%가 4.30으로 가장 낮은 값을 나타내었다.The pH value of the organic acid was gradually lowered as the organic acid content gradually increased as it matured. On the 100th day of fermentation, the highest value was GBP 10% 4.76 and GB 20% was the lowest at 4.30.

염도는 숙성 100일째 GB 5%구간이 3.30으로 가장 높은 값을 나타내었고 GB 20%구간이 2.27로 가장 낮은 값을 나타내었다.Salinity showed the highest value at 3.30 for the GB 5% section at 3.30 and lowest value at 2.27 for the GB 20% section.

색도 L값(명도)에 있어서 숙성초기부터 100일째까지 비슷한 값을 나타내고 있으며, a값(적색도)은 숙성 70, 100일째 비슷한 값을 보여주고 있다. b값(황색도)은 숙성기간 40일 이후에는 점차 증가하는 경향을 보여 숙성 100일째 GB 20%가 23.14로 가장 높은 값을 나타내었고 GB 15%가 15.90으로 가장 낮은 값을 나타내었다.The chromaticity L value (brightness) shows similar values from the early stage of fermentation to the 100th day, and the a value (redness) shows similar values on the 70th and 100th days of aging. The b value (yellowness) tended to increase gradually after 40 days of fermentation, and at the 100th day of fermentation, GB 20% showed the highest value of 23.14 and GB 15% showed the lowest value of 15.90.

총페놀은 숙성 100일째에는 70일째 값보다 큰 폭으로 감소하는 경향을 보였는데 GB 20%가 98.54로 가장 낮은 값을 나타내었고 GB 5%가 129.21로 가장 높은 값을 보여 주었다.Total phenols tended to decrease by more than 70 days at 100 days of fermentation, with 20% of GB showing the lowest value of 98.54 and 5% of GB showing the highest value of 129.21.

DPPH radical 소거 활성능에서 숙성 100일째에는 GBP 10%가 80.88로 가장 높은 값을 나타내었으며 GB 15%가 73.55로 가장 낮은 값을 나타내었다.On the 100th day of aging, the highest value of DPPH radical scavenging activity was GBP 10% at 80.88 and GB 15% at 73.55.

유기산 함량 분석결과 전반적으로 숙성기간이 길어질수록 유기산의 함량은 조금씩 증가하는 경향을 보여주고 있으며 유기산 중 lactic acid가 가장 많이 함유되어 있는 것으로 나타났으며 tartaric acid, oxalic acid, malic acid, acetic acid, citric acid, succinic acid가 비슷한 함량으로 함유되어 있는 것으로 나타났다.As a result of the analysis of the content of organic acid, the content of organic acid tended to increase little by little as the maturation period was longer, and it showed that lactic acid was the most contained among the organic acids and tartaric acid, oxalic acid, malic acid, acetic acid, citric acid and succinic acid were found to contain similar amounts.

천마 고추장에 함유된 유리 아미노산은 약 28종 함유되어 있는 것으로 나타났으며 천마 된장보다는 약 5종이 적은 것으로 나타났다. 유리아미노산 중 가장 많이 함유된 것은 Pro이고 다음으로 Asn이며, Ala, Val, Glu, Ile, Leu, Phe,가 비슷한 함량으로 미량 함유되어 있는 것으로 나타났다.About 28 kinds of free amino acids contained in Chunma Gochujang appeared to be about 5 kinds less than Chunma Doenjang. The highest content of free amino acids was Pro, followed by Asn, and Ala, Val, Glu, Ile, Leu, Phe, were found to contain similar amounts of traces.

천마고추장의 무기질 함량은 Na>K>Mg>Ca의 순으로 함유되어 있는 것으로 나타났으며, Co, Cu, Mo의 경우는 미량 함유되어 있는 것으로 나타났다. Ca의 경우 control이 357.436으로 가장 높은 값을 나타냈으며, Fe는 GB 20%가 가장 높은 것으로 나타났다. K 함유량에서는 GB 5%가 4766.68, Mg, Zn의 경우 control이 각각 567.578, 8.677로 가장 많이 함유하고 있는 것으로 나타났다.The mineral content of Chunma Gochujang was found in the order of Na> K> Mg> Ca, and in the case of Co, Cu, and Mo, it was found to contain a small amount. In the case of Ca, the control showed the highest value with 357.436, and Fe showed the highest with 20% of GB. For K content, GB 5% contained 4766.68, Mg and Zn, 567.578 and 8.677, respectively.

관능검사 결과, 10% 이상 첨가되는 구간인 GB 15%, GB 20%는 천마의 향과 맛이 굉장히 강해 일반인들에게 거부감을 주었다. 이상 결과에서, 천마고추장은 전통고추장을 제외하고 전반적으로 가장 높은 점수를 획득한 GB 10% 구간을 최종 배합비로 선택하였다.
As a result of the sensory test, GB 15% and GB 20%, which were added more than 10%, were very strong in the smell and taste of the cheonma, giving the public a sense of rejection. In the above results, the Cheonma Gochujang was selected as the final blending ratio of the GB 10% section, which obtained the highest overall score except the traditional pepper paste.

Figure pat00018

Figure pat00018

본 발명에 따른 천마고추장은 위와 같은 실험데이터를 거쳐 실제로 제품화하고 그 포장디자인까지 완료하였는바, 제품사진을 참고자료로 기재한다.
Cheonma pepper paste according to the present invention was actually manufactured through the above experimental data and the packaging design is completed, the product photograph is described as a reference.

사진 1. 천마 고추장을 제조하여 포장한 제품 사진Picture 1. Product picture of cheonma gochujang made and packed

Figure pat00019
Figure pat00019

Claims (6)

진공동결 건조한 천마분말로 만든 천마물엿 성분을 이용하여 제조하는 것을 특징으로 하는,
천마고추장의 제조방법.
Characterized in that the manufacturing using the cheonma syrup made from vacuum freeze dried cheonma powder,
Manufacturing method of cheonma pepper.
(1) 찹쌀을 세척하여 물에 불린 다음 갈아서 분말화한 다음, 상기 분쇄한 찹쌀분말을 엿기름액에 넣어 당화하고 그 여과한 액체를 40~50°Brix가 될 때까지 끓여서 농축하고 냉각시켜 찹쌀물엿을 제조하는 단계;
(2) 상기 (1) 단계와는 별도로, 진공동결건조한 천마분말을 엿기름액에 넣어 당화하고 그 여과한 액체를 40~50°Brix가 될 때까지 끓여서 농축하고 냉각시켜 천마물엿을 제조하는 단계;와
(3) 상기 찹쌀물엿과 천마물엿을 섞고 다시 거기에 고춧가루, 메줏가루 및 소금을 넣어 고추장을 제조하는 단계;로 이루어지는 것을 특징으로 하는,
천마고추장의 제조방법.
(1) Wash the glutinous rice, soak it in water, grind it, and powder it. Put the crushed glutinous rice powder into malt liquid, saccharify it, boil the filtered liquid until 40 ~ 50 ° Brix, concentrate it, and cool it. Preparing a;
(2) separately from the step (1), the step of vacuum freeze dried Cheonma powder into malt liquid and saccharified and boiled the filtered liquid until 40 ~ 50 ° Brix to concentrate and cool to prepare the Cheonma syrup; Wow
(3) mixing the glutinous rice syrup and cheonma syrup and adding red pepper powder, buckwheat flour and salt therein to prepare red pepper paste;
Manufacturing method of cheonma pepper.
제2항에 있어서,
상기 각 단계들을 통해 투입되는 찹쌀:천마분말:고춧가루:메줏가루:소금의 중량비는 5.4 : 0.5~0.7 : 5.9~6.1 : 2.4~2.6 : 1.9~2.1 인 것을 특징으로 하는,
천마고추장의 제조방법.
The method of claim 2,
The weight ratio of glutinous rice: Cheonma powder: red pepper powder: buckwheat powder: salt is added in each of the above steps is 5.4: 0.5-0.7: 5.9-6.1: 2.4-2.6: 1.9-2.1,
Manufacturing method of cheonma pepper.
제3항에 있어서,
상기 각 단계들을 통해 투입되는 찹쌀:천마분말:고춧가루:메줏가루:소금의 중량비는 5.4 : 0.6 : 6.0 : 2.5 : 2.0 인 것을 특징으로 하는,
천마고추장의 제조방법.
The method of claim 3,
The weight ratio of the glutinous rice: Cheonma powder: red pepper powder: powdered powder: salt: salt is 5.4: 0.6: 6.0: 2.5: 2.0, which are added through the respective steps,
Manufacturing method of cheonma pepper.
진공동결 건조한 천마분말로 만든 천마물엿 성분을 이용하여 제조되는 것을 특징으로 하는,
천마고추장.
Characterized in that the prepared using the cheonma syrup component made from vacuum freeze dried cheonma powder,
Cheonma red pepper paste.
제1항 내지 제4항 중 어느 한 항의 제조방법에 의하여 제조되는 것을 특징으로 하는,
천마고추장.
It is produced by the manufacturing method of any one of claims 1 to 4,
Cheonma red pepper paste.
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