CN110339338A - Hair restorer - Google Patents

Hair restorer Download PDF

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Publication number
CN110339338A
CN110339338A CN201910640960.7A CN201910640960A CN110339338A CN 110339338 A CN110339338 A CN 110339338A CN 201910640960 A CN201910640960 A CN 201910640960A CN 110339338 A CN110339338 A CN 110339338A
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parts
hair
stem cell
mesenchymal stem
conditioned medium
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杜忆华
靳莉
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Dongguan Natural Health Technology Co Ltd
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Dongguan Natural Health Technology Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19—Carboxylic acids, e.g. valproic acid
    • A61K31/195—Carboxylic acids, e.g. valproic acid having an amino group
    • A61K31/196—Carboxylic acids, e.g. valproic acid having an amino group the amino group being directly attached to a ring, e.g. anthranilic acid, mefenamic acid, diclofenac, chlorambucil
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
    • A61K31/4415—Pyridoxine, i.e. Vitamin B6
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/33—Fibroblasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/13—Coniferophyta (gymnosperms)
    • A61K36/14—Cupressaceae (Cypress family), e.g. juniper or cypress
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
    • A61K36/258—Panax (ginseng)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/489—Sophora, e.g. necklacepod or mamani
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A61K36/534—Mentha (mint)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A61K36/537—Salvia (sage)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
    • A61K38/06—Tripeptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/19—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles lyophilised, i.e. freeze-dried, solutions or dispersions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/08—Antiseborrheics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/14—Drugs for dermatological disorders for baldness or alopecia

Abstract

The present invention provides a kind of hair restorer, and the hair restorer includes fat mesenchymal stem cell conditioned medium, fibroblast excretion body, mannitol, trehalose and Dextran 40, wherein the fat mesenchymal stem cell is used with 20-100mJ/cm2The irradiation of UVB irradiation dose, fat mesenchymal stem cell after irradiation secretes the multiple biological activities factor, can stimulate papilla, activate hair follicle stem cells, reach hair tonic effect from inside to outside；The present invention also provides a kind of hair growth composition comprising nutritional agents and the hair restorer, has significant curative effect, use easy to spread to androgenetic alopecia, alopecia seborrheica, pelada and lipsotrichia.

Description

Hair restorer
Technical field
The present invention relates to skin care fields, and in particular to a kind of hair restorer and its application.
Background technique
As social pressures constantly increase and the accelerating rhythm of life, the continuous deterioration of environment and undesirable diet are practised Used, also growing day by day with our non-health, inferior health, Chinese hair loss patient is more and more, and the alopecia age shifts to an earlier date , 30-40 years old people have and much have a depilation phenomenon.Hair loss patient should drink unboiled water or the food containing abundant irony, lean meat, core more Peach, sesame, egg, spinach, cabbage, celery, fruit etc. are all optimal treatment food.Alopecia or baldpate, scalp It has all hardened, above-mentioned food helps to soften scalp.Motherland's traditional Chinese medicine thinks the cause of disease of alopecia mainly in kidney, if liver kidney Two empty insufficiency of vital energy and blood, the blood circulation of whole body is just weak, the powerless highest point " crown " that nutriment is transported to person upright, Hair follicle cannot nourish on head, gradually atrophy, will cause alopecia, " kidney storing essence, main reproduction, Hua Fa ", " hair be blood it It is remaining ", it is believed that the kidney being the origin of congenital constitution, product of the hair for blood, kidney storing essence, blood stored in the liver, and essence and blood sharing the same origin mutually converts, and the two lacks one It can not.
The phenomenon that alopecia is epilation is divided into physiological and pathologic.Physiologic alopecia finger hair normally falls off. Pathologic alopecia refers to that hair is abnormal or excessive and falls off.The cardinal symptom of alopecia has hair greasy, as polishing；Also have It is shrivelled to send out fluffy, lack gloss；There is the fixed difficult de- or canescence scales of skin that peel off of the faint yellow scales of skin that peel off to fly upward, conscious itch.If male is de- Hair, mainly front and overhead, with temples toward moving up, the hair sparse of front and top turns yellow the hairline of forehead, softens, Make a piece of baldness or some fine hair at the top of volume eventually;Portion, hair become sparse to female alopecia overhead, but not completely sheet of de- It falls.
Alopecia first has to distinguish type, and general alopecia can be divided into two kinds of fundamental types, permanent caused by being damaged as hair follicle Property alopecia, and as the hair follicle short time it is impaired caused by temporary alopecia, the male of permanent alopecia, that is, common is bald, certain The bald rate of European countries, male is up to 40%.
The hair loss process of permanent alopecia (i.e. male pattern alopecia) gradually generates, when beginning, the hair of head frons Then the obvious retraction in edge, overhead exiguous human hair gradually develop, can finally develop to only remaining back of head, and both sides of head one is enclosed Thin hair, main cause have three: inherent cause, and the shortage or imbalance of male sex hormone, are too obese, separately in blood circulation Outside, the scar that multiple dermatosis or skin damage leave, born hair follicle development is bad, and the level of protona is higher than normal in blood Crowd and chemical substance or the physical cause grievous injury caused by hair follicle can cause permanent alopecia.
Temporary alopecia often as caused by disease high fever, but, according to X-ray, is taken in metal (such as thallium, tin and arsenic) or is taken the photograph Enter poisonous substance, malnutritive, certain skin diseases for causing inflammation, chronic wasting disease and endocrine disorder etc. can also cause temporarily The alopecia of when property.
The drug of clinical treatment alopecia at present is mainly Finasteride and minoxidil, although using rear some patientss disease Shape makes moderate progress, but also varies with each individual, and few patients also will appear adverse reaction, can not fundamentally solve asking for alopecia Topic.Also some patientss select hair transplant, but hair transplant needs to be implanted into again from itself rear pillow part healthy hair area materials to forehead, the crown Etc. hair loss areas, it is this " to tear open for the patient sparse for hair itself although there is part hair follicle that can grow at new position The way that Dong Qiang mends Xi Qiang " not can increase itself hair follicle, and the operating time is long, there is serious pain sensation, expensive, and postoperative effect Fruit cannot be guaranteed, it is also possible to occur with the complication such as infection, swelling, therefore develop effective hair tonic medicament newly have it is huge Clinical meaning.In the prior art, to the treatment of alopecia, there is hair restorer various in style, educate hair agent, relative to chemotherapeutic drug Object is more toxic, the big defect of skin irritation, the prescription of Chinese materia medica preparation with it without any toxicity and side effects the advantages of by user Welcome.
CN102727595A discloses a kind of Chinese medicine compound prescription hair restorer and preparation method thereof, the Chinese medicine compound prescription hair restorer according to Parts by weight meter is composed of the following components: 150-200 parts of Eclipta prostrata, 80-120 parts of RADIX POLYGONI MULTIFLORI PREPARATA, and 60-120 parts of lopseed, safflower 60- 120 parts, 60-120 parts of cacumen biotae, 40-100 parts of psoralea corylifolia；Preparation method is to weigh the Chinese medicine compound prescription formula for growing hair, powder It is broken, it is sufficiently mixed, the aqueous medium for being equivalent to 15-25 times of volume of mixture is added in the resulting mixture, extraction is collected in extraction Liquid；Gained extract liquor is concentrated, hair restorer is obtained.The effective component of the hair restorer is extracted using conventional solvent, when extraction Between it is longer, the active constituent content in final products is relatively low.
With going deep into for research, people have clearer understanding to alopecia mechanism, and scientist generally recognizes hair again The raw regulation by hair follicle stem cells function and its surrounding microenvironment.Stem cell is a kind of pluripotency with the of self-replication capacity Cell has the ability to Various Tissues cell differentiation, and the performance for this ability of hair follicle stem cells that hair occurs needs To be unfolded in good microenvironment, such as sufficient nutrition supply.Therefore exploitation activation hair follicle stem cells and its microenvironment are repaired Multiple hair regeneration product becomes the development trend of hair growth drug.
Accordingly, the present invention cooperates fibroblast excretion body by fat mesenchymal stem cell conditioned medium, from source Upper stimulation hair relevant cell regeneration, further stimulates papilla, activates hair follicle stem cells, anti-hair papilla cell apoptosis, starting The signal of hair regeneration to start new growth cycle of hair, while providing nutrition to hair follicle, to solve alopecia from root Problem.
Summary of the invention
In view of this, the hair restorer passes through fat mesenchymal stem cell the purpose of the present invention is to provide a kind of hair restorer Conditioned medium cooperates fibroblast excretion body, stimulates hair relevant cell to regenerate from source, further stimulates papilla, Hair follicle stem cells are activated, hair papilla cell apoptosis is fought, starts the signal of hair regeneration, to start new hair cycle, together When give hair follicle provide nutrition, can solve the problems, such as alopecia from root.In another aspect, by adjusting component in hair restorer type and Parts by weight, the present invention further improve the hair-growing effects of hair restorer.
For this purpose, the present invention provides following technical schemes.
In a first aspect, the hair restorer is trained comprising fat mesenchymal stem cell condition the present invention provides a kind of hair restorer Nutrient solution, fibroblast excretion body, mannitol, trehalose and Dextran 40, wherein the fat mesenchymal stem cell uses With 20-100mJ/cm2UVB irradiation dose irradiation.
In a preferred embodiment, the parts by weight of the mannitol are 10-14, for example, the weight of the mannitol Number can be 10,10.5,11,11.5,12,12.5,13,13.5 or 14；
The parts by weight of the trehalose are 0.5-0.6, for example, the parts by weight of the trehalose can for 0.5,0.51, 0.52,0.53,0.54,0.55,0.56,0.57,0.58,0.59 or 0.6；
The parts by weight of the Dextran 40 are 0.4-0.5, for example, the parts by weight of the Dextran 40 can for 0.4, 0.41,0.42,0.43,0.44,0.45,0.46,0.47,0.48,0.49 or 0.5.
Second aspect, the present invention provides a kind of preparation methods of hair restorer, wherein the method comprises the steps of:
Step 1: preparing fat mesenchymal stem cell conditioned medium
1. the collection of fat mesenchymal stem cell conditioned medium: with 20-100mJ/cm2UVB irradiation dose irradiate 3-10 generation Fat mesenchymal stem cell, the fat mesenchymal stem cell after irradiation continue to cultivate, and fill between the fat of collection culture 48-72 hours Matter stem cell conditioned medium；
2. the removal of impurities of fat mesenchymal stem cell conditioned medium is concentrated: being filled using ultrafiltration concentration technology between the fat being collected into Matter stem cell conditioned medium is cleaned, is once concentrated, secondary concentration, is obtained secondary concentration liquid and is filled to get between the fat Matter stem cell conditioned medium.
Step 2: preparation fibroblast excretion body
1. cultivate adherent fibroblast to degrees of fusion 90%, culture solution supernatant is collected, and be extracted into using supercentrifugation Fibrocyte excretion body；
2. passing on after the cell dissociation retained, and continue to cultivate, collect supernatant, extracts fibroblast with supercentrifugation later Excretion body is to get the fibroblast excretion body.
Step 3: the fat mesenchymal stem cell that step 1 obtains is added in the fibroblast excretion body that step 2 obtains It in conditioned medium, stirs evenly, filtration sterilization；
Step 4: the mixture of mannitol, trehalose and Dextran 40 is added in step 3 obtained material, freezed after packing Drying is to get the hair restorer.
In a preferred embodiment, the parts by weight of the mannitol are 10-14, and the parts by weight of the trehalose are 0.5-0.6, the parts by weight of the Dextran 40 are 0.4-0.5.
In a preferred embodiment, the hair restorer is hair restorer of the present invention.
The third aspect, the present invention provides a kind of nutritional agents, the nutritional agents include ginseng, cacumen biotae, Radix Salviae Miltiorrhizae, kuh-seng, Peppery peppermint, p-aminobenzoic acid, palmitoyl tripeptide 3 and vitamin B6.
In a preferred embodiment, according to parts by weight, the nutritional agents includes:
2-7 parts of ginsengs, for example, the parts by weight of the ginseng can be 2,3,4,5,6 or 7；
3-8 portions of cacumen biotaes, for example, the parts by weight of the primary leaf in the side can be 3,4,5,6,7 or 8；
2-7 parts of Radix Salviae Miltiorrhizaes, for example, the parts by weight of the Radix Salviae Miltiorrhizae can be 2,3,4,5,6 or 7；
5-10 parts of kuh-sengs, for example, the parts by weight of the kuh-seng can be 5,6,7,8,9 or 10；
1-6 parts of peppery peppermints, for example, the parts by weight of the peppery peppermint can be 1,2,3,4,5 or 6；
1-5 parts of p-aminobenzoic acid, for example, the parts by weight of the p-aminobenzoic acid can be 1,2,3,4 or 5；
0.5-2 parts of palmitoyl tripeptide 3s, for example, the parts by weight of the palmitoyl tripeptide 3 can be 0.5,1,1.5 or 2；
With 0.1-2 parts of vitamin B6s, for example, the parts by weight of the vitamin B6 can be 0.1,0.5,1,1.5 or 2.
In preferred embodiment, according to parts by weight, the nutritional agents includes:
5 parts of ginsengs, 6 portions of cacumen biotaes, 4 parts of Radix Salviae Miltiorrhizaes, 7 parts of kuh-sengs, 2 parts of peppery peppermints, 2 parts of p-aminobenzoic acid, 1 part of palmitoyl tripeptide 3 and 0.5 part of vitamin B6.
Fourth aspect, the present invention provides a kind of preparation method of nutritional agents, the method is comprised the steps of:
Step 1: being put into extractor after ginseng, cacumen biotae, Radix Salviae Miltiorrhizae, kuh-seng, peppery peppermint are mixed, Traditional Chinese drug mixture is obtained；
Step 2: be added 5-10 times of Traditional Chinese drug mixture weight 75% ethyl alcohol 40-48 DEG C refluxing extraction 1.5-2.5 hours, filter out Medical fluid；
Step 3: again be added 5-10 times of Traditional Chinese drug mixture weight 75% ethyl alcohol 40-48 DEG C refluxing extraction 1.5-2.5 hours, Filter out medical fluid；
Step 4: merging the medical fluid of step 2 and step 3, being concentrated into relative to the density of water is 0.6-0.9；
Step 5: by butanediol: after water=1-3:1 ratio mixing, the medical fluid of step 4 is added, puts to room temperature and filters, be warming up to 43-48 DEG C, p-aminobenzoic acid, palmitoyl tripeptide 3 and vitamin B6 is added, stirs evenly.
5th aspect, the present invention provides a kind of hair growth composition, the composition includes the nutritional agents of safe and effective amount With the hair restorer as described in the present invention of safe and effective amount, wherein the nutritional agents include ginseng, cacumen biotae, Radix Salviae Miltiorrhizae, kuh-seng, Peppery peppermint, p-aminobenzoic acid, palmitoyl tripeptide 3 and vitamin B6.
In a preferred embodiment, according to parts by weight, the nutritional agents includes:
2-7 parts of ginsengs, 3-8 portions of cacumen biotaes, 2-7 parts of Radix Salviae Miltiorrhizaes, 5-10 parts of kuh-sengs, 1-6 parts of peppery peppermints, 1-5 parts of p-aminobenzoic acid, 0.5-2 parts of palmitoyl tripeptide 3s and 0.1-2 parts of vitamin B6s.
In preferred embodiment, according to parts by weight, the nutritional agents includes:
5 parts of ginsengs, 6 portions of cacumen biotaes, 4 parts of Radix Salviae Miltiorrhizaes, 7 parts of kuh-sengs, 2 parts of peppery peppermints, 2 parts of p-aminobenzoic acid, 1 part of palmitoyl tripeptide 3 and 0.5 part of vitamin B6.
In a preferred embodiment, the nutritional agents the preparation method is as follows:
Step 1: being put into extractor after ginseng, cacumen biotae, Radix Salviae Miltiorrhizae, kuh-seng and peppery peppermint are mixed, Traditional Chinese drug mixture is obtained；
Step 2: be added 5-10 times of Traditional Chinese drug mixture weight 75% ethyl alcohol 40-48 DEG C refluxing extraction 1.5-2.5 hours, filter out Medical fluid；
Step 3: again be added 5-10 times of Traditional Chinese drug mixture weight 75% ethyl alcohol 40-48 DEG C refluxing extraction 1.5-2.5 hours, Filter out medical fluid；
Step 4: merging the medical fluid of step 2 and step 3, being concentrated into relative to the density of water is 0.6-0.9；
Step 5: by butanediol: after water=1-3:1 ratio mixing, the medical fluid of step 4 is added, puts to room temperature and filters, be warming up to 43-48 DEG C, p-aminobenzoic acid, palmitoyl tripeptide 3 and vitamin B6 is added, stirs evenly.
6th aspect, provides a kind of pharmaceutical composition, and the composition includes the as described herein of safe and effective amount Nutritional agents and safe and effective amount hair restorer as described in the present invention.
In addition, additionally providing nutritional agents of the present invention and/or hair restorer of the present invention in preparation hair tonic combination Purposes in object.
And nutritional agents of the present invention and/or hair restorer of the present invention are provided in preparation pharmaceutical composition In purposes.
In the present invention, safe and effective amount refers to the combination of a certain amount of reagent, and the amount, which is enough significantly to induce, positive to be had Beneficial effect, preferably positive hair tonic beneficial effect include beneficial effect disclosed herein in form independent or combined, but institute The amount of stating is again sufficiently low to avoid serious side effect, i.e., reasonable effective hazard ratio is provided in the range of technical staff rationally judges.
Compared with the existing technology, the beneficial effects of the present invention are:
Hair restorer provided by the invention, fat mesenchymal stem cell used in the hair restorer is through 20-100mJ/cm2UVB irradiation After dose delivery, the fat mesenchymal stem cell after irradiation can secrete the multiple biological activities factor, such as vascular endothelial growth factor (vascular endothelial growth factor, VEGF), hepatocyte growth factor (hepatocyte growth Factor, HGF), stem cell factor (stem cell factor, SCF), basic fibroblast growth factor (fibroblast growth factor basic, bFGF), fibroblast growth factor (fibroblast growth Factor, FGF), keratinocyte growth factor (keratinocyte growth factor, KGF), platelet derived growth The factor (platelet-derived growth factor, PDGF), type-1 insulin like growth factor (insulin-like Growth factor 1, IGF-1), these growth factors are conducive to the activation of hair follicle stem cells；The fibroblast used Excretion body, which has, promotes hair follicle stem cells proliferation and migration, activates hair papilla cell, promotes revascularization, to anti-apoptotic Effect, to promote hair regeneration on source；Cooperate the mannitol of special ratios of the present invention, trehalose and Dextran 40 Mixture can preferably protect excretion body active, help hair regeneration as freeze drying protectant.
In addition, the present invention can further stimulate papilla by adjusting the type and parts by weight of component in hair restorer, Hair follicle stem cells are activated, hair tonic effect from inside to outside is reached.
Nutritional agents provided by the invention, ginseng used in the nutritional agents have activating microcirculation and removing stasis medicinal, expand subcutaneous capillary, Promote hair follicle root blood circulation, improves the effect of microcirculation, to achieve the purpose that hair tonic；The cacumen biotae used is always exactly The good medicine of growing and blacking hair, active constituent are volatile oil, there is the effect of cooling blood and hemostasis；The Radix Salviae Miltiorrhizae used, which has, expands subcutaneous capillary Blood vessel improves microcirculation, reinforces hair follicle nutrition, promotes the effect of hair regeneration, and fat-soluble effective component tanshinone has slow The estrogenic activity of sum, and can antibacterial dispel rouge, have antiandrogen, adjust immune function and anti-inflammatory effect；And in kuh-seng Flavone compound, not only there is apparent antibacterial action, moreover it is possible to which the inhibition 5α-reductase activity of conspicuousness inhibits testosterone It is changed into protona, to extend the growth period of hair, achievees the purpose that hair growing；And the ethanol extract of peppery peppermint is also 5α-reductase can be effectively inhibited and sebum is formed, testosterone is blocked to be changed into protona, also there is balance scalp grease point The effect of secreting, adjusting microenvironment；The vitamin B6 used can adjust skin-nourishing health, participate in property hair cycle circulation；Make On the one hand palmitoyl tripeptide 3 can activate hair follicle stem cells, promote hair root cytothesis division, promote hair growth factor table It reaches, helps hair regeneration, papilla and hair follicle collagen on the other hand can be promoted to produce, keep regenerated hair firm strong It is strong.Therefore, in nutritional agents of the present invention each substance collocation, can promote inside and outside hair follicle hair generate, being capable for the treatment of both manifestation and root cause of disease.
In addition, the present invention can further promote hair raw by adjusting the type and parts by weight of component in nutritional agents Long factor expression further can promote hair to generate.
Hair growth composition provided by the invention has significant curative effect to androgenetic alopecia, alopecia seborrheica, pelada and lipsotrichia, Curative for effect, safely controllable, patient is easy to receive, and use easy to spread is safe and non-toxic, is worth clinical promotion and application.
Specific embodiment
Below in conjunction with specific embodiment and embodiment, it is specifically described the present invention, advantages of the present invention and various effects It thus more will clearly present.It will be understood by those skilled in the art that these specific embodiments and embodiment are for illustrating The present invention is not intended to limit the present invention.
Throughout the specification, unless otherwise specified, terms used herein are interpreted as usual in this field Used meaning.As a result, unless otherwise defined, all technical and scientific terms used herein has leads with belonging to the present invention The identical meaning of the general understanding of field technique personnel.Contradiction if it exists, this specification are preferential.
The present invention is described in more detail by the following examples, but the present invention is not limited to these Examples.
Embodiment 1
The present embodiment provides a kind of nutritional agents, the nutritional agents includes the component of following parts by weight:
2 parts of ginsengs, 3 portions of cacumen biotaes, 2 parts of Radix Salviae Miltiorrhizaes, 5 parts of kuh-sengs, 1 part of peppery peppermint, 1 part of p-aminobenzoic acid, 0.5 part of palmitoyl tripeptide 3 and 0.1 part of vitamin B6.
The present embodiment nutritional agents the preparation method is as follows:
Step 1: being put into extractor after 2 parts of ginsengs, 3 portions of cacumen biotaes, 2 parts of Radix Salviae Miltiorrhizaes, 5 parts of kuh-sengs, 1 part of peppery peppermint are mixed, obtain To Traditional Chinese drug mixture；
Step 2: be added 5 times of Traditional Chinese drug mixture weight 75% ethyl alcohol 40 DEG C refluxing extraction 1.5 hours, filter out medical fluid；
Step 3: again be added 5 times of Traditional Chinese drug mixture weight 75% ethyl alcohol 40 DEG C refluxing extraction 1.5 hours, filter out medical fluid；
Step 4: merging the medical fluid of step 2 and step 3, being concentrated into relative to the density of water is 0.6；
Step 5: by butanediol: after water=1:1 ratio mixing, the medical fluid of step 4 is added, puts to room temperature and filters, be warming up to 43 DEG C, 1 part of p-aminobenzoic acid, 0.5 part of palmitoyl tripeptide 3 and 0.1 part of vitamin B6 is added, stirs evenly.
Embodiment 2
The present embodiment provides a kind of nutritional agents, the nutritional agents includes the component of following parts by weight:
7 parts of ginsengs, 8 portions of cacumen biotaes, 7 parts of Radix Salviae Miltiorrhizaes, 10 parts of kuh-sengs, 6 parts of peppery peppermints, 5 parts of p-aminobenzoic acid, 2 parts of palmitoyl tripeptide 3s and 2 Part vitamin B6.
The present embodiment nutritional agents the preparation method is as follows:
Step 1: be put into extractor after 7 parts of ginsengs, 8 portions of cacumen biotaes, 7 parts of Radix Salviae Miltiorrhizaes, 10 parts of kuh-sengs, 6 parts of peppery peppermints are mixed, Obtain Traditional Chinese drug mixture；
Step 2: be added 10 times of Traditional Chinese drug mixture weight 75% ethyl alcohol 48 DEG C refluxing extraction 2.5 hours, filter out medical fluid；
Step 3: again be added 10 times of Traditional Chinese drug mixture weight 75% ethyl alcohol 48 DEG C refluxing extraction 2.5 hours, filter out medical fluid；
Step 4: merging the medical fluid of step 2 and step 3, being concentrated into relative to the density of water is 0.9；
Step 5: by butanediol: after water=3:1 ratio mixing, the medical fluid of step 4 is added, puts to room temperature and filters, be warming up to 47 DEG C, 5 parts of p-aminobenzoic acid, 2 parts of palmitoyl tripeptide 3s and 2 parts of vitamin B6s are added, stir evenly.
Embodiment 3
The present embodiment provides a kind of nutritional agents, the nutritional agents includes the component of following parts by weight:
5 parts of ginsengs, 6 portions of cacumen biotaes, 4 parts of Radix Salviae Miltiorrhizaes, 7 parts of kuh-sengs, 2 parts of peppery peppermints, 2 parts of p-aminobenzoic acid, 1 part of palmitoyl tripeptide 3 and 0.5 part of vitamin B6.
The present embodiment nutritional agents the preparation method is as follows:
Step 1: being put into extractor after 5 parts of ginsengs, 6 portions of cacumen biotaes, 4 parts of Radix Salviae Miltiorrhizaes, 7 parts of kuh-sengs, 2 parts of peppery peppermints are mixed, obtain To Traditional Chinese drug mixture；
Step 2: be added 8 times of Traditional Chinese drug mixture weight 75% ethyl alcohol 45 DEG C refluxing extraction 2 hours, filter out medical fluid；
Step 3: again be added 8 times of Traditional Chinese drug mixture weight 75% ethyl alcohol 45 DEG C refluxing extraction 2 hours, filter out medical fluid；
Step 4: merging the medical fluid of step 2 and step 3, being concentrated into relative to the density of water is 0.6；
Step 5: by butanediol: after water=2:1 ratio mixing, the medical fluid of step 4 is added, puts to room temperature and filters, be warming up to 45 DEG C, 2 parts of p-aminobenzoic acid, 1 part of palmitoyl tripeptide 3 and 0.5 part of vitamin B6 is added, stirs evenly.
Embodiment 4
The present embodiment provides a kind of hair restorer, the hair restorer includes the component of following parts by weight:
Fat mesenchymal stem cell conditioned medium, fibroblast excretion body, 10 portions of mannitol, 0.5 portion of trehalose and 0.4 part Dextran 40.
The present embodiment hair restorer the preparation method is as follows:
Step 1: preparing fat mesenchymal stem cell conditioned medium
1. the collection of fat mesenchymal stem cell conditioned medium: with 20mJ/cm2UVB irradiation dose irradiate 3 fat subsitutes between fill Matter stem cell, the fat mesenchymal stem cell after irradiation continue to cultivate, and collect the fat mesenchymal stem cell condition training of culture 48h Nutrient solution is stored in 4 DEG C after centrifugation；
2. the removal of impurities of fat mesenchymal stem cell conditioned medium is concentrated: being filled using ultrafiltration concentration technology between the fat being collected into Matter stem cell conditioned medium is cleaned, is once concentrated, secondary concentration, is obtained secondary concentration liquid and is filled to get between the fat Matter stem cell conditioned medium.
Step 2: preparation fibroblast excretion body
1. cultivate adherent fibroblast to degrees of fusion 90%, culture solution supernatant is collected, and outer using supercentrifugation extraction Secrete body；
2. passing on after the cell dissociation retained, and continue to cultivate, collect supernatant, extracts fibroblast with supercentrifugation later Excretion body, repeatedly to get the fibroblast excretion body.
Step 3: the fat mesenchymal stem cell that step 1 obtains is added in the fibroblast excretion body that step 2 obtains It in conditioned medium, stirs evenly, degerming is filtered using miillpore filter；
Step 4: carrying out total protein concentration measurement, total protein concentration 0.5-2mg/ to step 3 obtained material using BCA method mL；
Step 5: carrying out pH value measurement, pH value 6.5-8 to step 3 obtained material using pH meter.
Step 6: 10 portions of mannitol, 0.5 portion of trehalose and 0.4 part of Dextran 40 are added in step 3 obtained material Mixture, after packing freeze-drying to get the hair restorer, storage at normal temperature is spare.
Embodiment 5
The present embodiment provides a kind of hair restorer, the hair restorer includes the component of following parts by weight:
Fat mesenchymal stem cell conditioned medium, fibroblast excretion body, 14 portions of mannitol, 0.6 portion of trehalose and 0.5 part Dextran 40.
The present embodiment hair restorer the preparation method is as follows:
Step 1: preparing fat mesenchymal stem cell conditioned medium
1. the collection of fat mesenchymal stem cell conditioned medium: with 20mJ/cm2UVB irradiation dose irradiate 10 fat subsitutes between Mesenchymal stem cells, the fat mesenchymal stem cell after irradiation continue to cultivate, and collect the fat mesenchymal stem cell condition of culture 72h Culture solution is stored in 4 DEG C after centrifugation；
2. the removal of impurities of fat mesenchymal stem cell conditioned medium is concentrated: being filled using ultrafiltration concentration technology between the fat being collected into Matter stem cell conditioned medium is cleaned, is once concentrated, secondary concentration, is obtained secondary concentration liquid and is filled to get between the fat Matter stem cell conditioned medium.
Step 2: preparation fibroblast excretion body
1. cultivate adherent fibroblast to degrees of fusion 90%, culture solution supernatant is collected, and be extracted into using supercentrifugation Fibrocyte excretion body；
2. passing on after the cell dissociation retained, and continue to cultivate, collect supernatant, extracts fibroblast with supercentrifugation later Excretion body, repeatedly to get the fibroblast excretion body.
Step 3: the fat mesenchymal stem cell that step 1 obtains is added in the fibroblast excretion body that step 2 obtains It in conditioned medium, stirs evenly, degerming is filtered using miillpore filter；
Step 4: carrying out total protein concentration measurement, total protein concentration 0.5-2mg/ to step 3 obtained material using BCA method mL；
Step 5: carrying out pH value measurement, pH value 6.5-8 to step 3 obtained material using pH meter.
Step 6: 14 portions of mannitol, 0.6 portion of trehalose and 0.5 part of Dextran 40 are added in step 3 obtained material Mixture, after packing freeze-drying to get the hair restorer, storage at normal temperature is spare.
Embodiment 6
The present embodiment provides a kind of hair restorer, the hair restorer includes the component of following parts by weight:
Fat mesenchymal stem cell conditioned medium, fibroblast excretion body, 12 portions of mannitol, 0.5 portion of trehalose and 0.4 part Dextran 40.
The present embodiment hair restorer the preparation method is as follows:
Step 1: preparing fat mesenchymal stem cell conditioned medium
1. the collection of fat mesenchymal stem cell conditioned medium: with 20mJ/cm2UVB irradiation dose irradiate 5 fat subsitutes between fill Matter stem cell, the fat mesenchymal stem cell after irradiation continue to cultivate, and collect the fat mesenchymal stem cell condition training of culture 48h Nutrient solution is stored in 4 DEG C after centrifugation；
2. the removal of impurities of fat mesenchymal stem cell conditioned medium is concentrated: being filled using ultrafiltration concentration technology between the fat being collected into Matter stem cell conditioned medium is cleaned, is once concentrated, secondary concentration, is obtained secondary concentration liquid and is filled to get between the fat Matter stem cell conditioned medium.
Step 2: preparation fibroblast excretion body
1. cultivate adherent fibroblast to degrees of fusion 90%, culture solution supernatant is collected, and be extracted into using supercentrifugation Fibrocyte excretion body；
2. passing on after the cell dissociation retained, and continue to cultivate, collect supernatant, extracts fibroblast with supercentrifugation later Excretion body, repeatedly to get the fibroblast excretion body.
Step 3: the fat mesenchymal stem cell that step 1 obtains is added in the fibroblast excretion body that step 2 obtains It in conditioned medium, stirs evenly, degerming is filtered using miillpore filter；
Step 4: carrying out total protein concentration measurement, total protein concentration 0.5-2mg/ to step 3 obtained material using BCA method mL；
Step 5: carrying out pH value measurement, pH value 6.5-8 to step 3 obtained material using pH meter.
Step 6: 12 portions of mannitol, 0.5 portion of trehalose and 0.4 part of Dextran 40 are added in step 3 obtained material Mixture, after packing freeze-drying to get the hair restorer, storage at normal temperature is spare.
Embodiment 7
The present embodiment provides a kind of hair growth composition, the hair growth composition includes nutritional agents and hair restorer, wherein the nutrition Agent includes the component of following parts by weight:
2 parts of ginsengs, 3 portions of cacumen biotaes, 2 parts of Radix Salviae Miltiorrhizaes, 5 parts of kuh-sengs, 1 part of peppery peppermint, 1 part of p-aminobenzoic acid, 0.5 part of palmitoyl tripeptide 3 and 0.1 part of vitamin B6.
The nutritional agents the preparation method is as follows:
Step 1: being put into extractor after 2 parts of ginsengs, 3 portions of cacumen biotaes, 2 parts of Radix Salviae Miltiorrhizaes, 5 parts of kuh-sengs, 1 part of peppery peppermint are mixed, obtain To Traditional Chinese drug mixture；
Step 2: be added 5 times of Traditional Chinese drug mixture weight 75% ethyl alcohol 40 DEG C refluxing extraction 1.5 hours, filter out medical fluid；
Step 3: again be added 5 times of Traditional Chinese drug mixture weight 75% ethyl alcohol 40 DEG C refluxing extraction 1.5 hours, filter out medical fluid；
Step 4: merging the medical fluid of step 2 and step 3, being concentrated into relative to the density of water is 0.6；
Step 5: by butanediol: after water=1:1 ratio mixing, the medical fluid of step 4 is added, puts to room temperature and filters, be warming up to 43 DEG C, 1 part of p-aminobenzoic acid, 0.5 part of palmitoyl tripeptide 3 and 0.1 part of vitamin B6 is added, stirs evenly.
The hair restorer includes the component of following parts by weight:
Fat mesenchymal stem cell conditioned medium, fibroblast excretion body, 10 portions of mannitol, 0.5 portion of trehalose and 0.4 part Dextran 40.
The hair restorer the preparation method is as follows:
Step 1: preparing fat mesenchymal stem cell conditioned medium
1. the collection of fat mesenchymal stem cell conditioned medium: with 20mJ/cm2UVB irradiation dose irradiate 3 fat subsitutes between fill Matter stem cell, the fat mesenchymal stem cell after irradiation continue to cultivate, and collect the fat mesenchymal stem cell condition training of culture 48h Nutrient solution is stored in 4 DEG C after centrifugation；
2. the removal of impurities of fat mesenchymal stem cell conditioned medium is concentrated: being filled using ultrafiltration concentration technology between the fat being collected into Matter stem cell conditioned medium is cleaned, is once concentrated, secondary concentration, is obtained secondary concentration liquid and is filled to get between the fat Matter stem cell conditioned medium.
Step 2: preparation fibroblast excretion body
1. cultivate adherent fibroblast to degrees of fusion 90%, culture solution supernatant is collected, and be extracted into using supercentrifugation Fibrocyte excretion body；
2. passing on after the cell dissociation retained, and continue to cultivate, collect supernatant, extracts fibroblast with supercentrifugation later Excretion body, repeatedly to get the fibroblast excretion body.
Step 3: the fat mesenchymal stem cell that step 1 obtains is added in the fibroblast excretion body that step 2 obtains It in conditioned medium, stirs evenly, degerming is filtered using miillpore filter；
Step 4: carrying out total protein concentration measurement, total protein concentration 0.5-2mg/ to step 3 obtained material using BCA method mL；
Step 5: carrying out pH value measurement, pH value 6.5-8 to step 3 obtained material using pH meter.
Step 6: 10 portions of mannitol, 0.5 portion of trehalose and 0.4 part of Dextran 40 are added in step 3 obtained material Mixture, after packing freeze-drying to get the hair restorer, storage at normal temperature is spare.
Embodiment 8
The present embodiment provides a kind of hair growth composition, the hair growth composition includes nutritional agents and hair restorer, wherein
The nutritional agents includes the component of following parts by weight:
7 parts of ginsengs, 8 portions of cacumen biotaes, 7 parts of Radix Salviae Miltiorrhizaes, 10 parts of kuh-sengs, 6 parts of peppery peppermints, 5 parts of p-aminobenzoic acid, 2 parts of palmitoyl tripeptide 3s and 2 Part vitamin B6.
The nutritional agents the preparation method is as follows:
Step 1: be put into extractor after 7 parts of ginsengs, 8 portions of cacumen biotaes, 7 parts of Radix Salviae Miltiorrhizaes, 10 parts of kuh-sengs, 6 parts of peppery peppermints are mixed, Obtain Traditional Chinese drug mixture；
Step 2: be added 10 times of Traditional Chinese drug mixture weight 75% ethyl alcohol 48 DEG C refluxing extraction 2.5 hours, filter out medical fluid；
Step 3: again be added 10 times of Traditional Chinese drug mixture weight 75% ethyl alcohol 48 DEG C refluxing extraction 2.5 hours, filter out medical fluid；
Step 4: merging the medical fluid of step 2 and step 3, being concentrated into relative to the density of water is 0.9；
Step 5: by butanediol: after water=3:1 ratio mixing, the medical fluid of step 4 is added, puts to room temperature and filters, be warming up to 47 DEG C, 5 parts of p-aminobenzoic acid, 2 parts of palmitoyl tripeptide 3s and 2 parts of vitamin B6s are added, stir evenly.
The hair restorer includes the component of following parts by weight:
Fat mesenchymal stem cell conditioned medium, fibroblast excretion body, 14 portions of mannitol, 0.6 portion of trehalose and 0.5 part Dextran 40.
The hair restorer the preparation method is as follows:
Step 1: preparing fat mesenchymal stem cell conditioned medium
1. the collection of fat mesenchymal stem cell conditioned medium: with 20mJ/cm2UVB irradiation dose irradiate 10 fat subsitutes between Mesenchymal stem cells, the fat mesenchymal stem cell after irradiation continue to cultivate, and collect the fat mesenchymal stem cell condition of culture 72h Culture solution is stored in 4 DEG C after centrifugation；
2. the removal of impurities of fat mesenchymal stem cell conditioned medium is concentrated: being filled using ultrafiltration concentration technology between the fat being collected into Matter stem cell conditioned medium is cleaned, is once concentrated, secondary concentration, is obtained secondary concentration liquid and is filled to get between the fat Matter stem cell conditioned medium.
Step 2: preparation fibroblast excretion body
1. cultivate adherent fibroblast to degrees of fusion 90%, culture solution supernatant is collected, and be extracted into using supercentrifugation Fibrocyte excretion body；
2. passing on after the cell dissociation retained, and continue to cultivate, collect supernatant, extracts fibroblast with supercentrifugation later Excretion body, repeatedly to get the fibroblast excretion body.
Step 3: the fat mesenchymal stem cell that step 1 obtains is added in the fibroblast excretion body that step 2 obtains It in conditioned medium, stirs evenly, degerming is filtered using miillpore filter；
Step 4: carrying out total protein concentration measurement, total protein concentration 0.5-2mg/ to step 3 obtained material using BCA method mL；
Step 5: carrying out pH value measurement, pH value 6.5-8 to step 3 obtained material using pH meter.
Step 6: 14 portions of mannitol, 0.6 portion of trehalose and 0.5 part of Dextran 40 are added in step 3 obtained material Mixture, after packing freeze-drying to get the hair restorer, storage at normal temperature is spare.
Embodiment 9
The present embodiment provides a kind of hair growth composition, the hair growth composition includes nutritional agents and hair restorer, wherein
The nutritional agents includes the component of following parts by weight:
5 parts of ginsengs, 6 portions of cacumen biotaes, 4 parts of Radix Salviae Miltiorrhizaes, 7 parts of kuh-sengs, 2 parts of peppery peppermints, 2 parts of p-aminobenzoic acid, 1 part of palmitoyl tripeptide 3 and 0.5 part of vitamin B6.
The nutritional agents the preparation method is as follows:
Step 1: being put into extractor after 5 parts of ginsengs, 6 portions of cacumen biotaes, 4 parts of Radix Salviae Miltiorrhizaes, 7 parts of kuh-sengs, 2 parts of peppery peppermints are mixed, obtain To Traditional Chinese drug mixture；
Step 2: be added 8 times of Traditional Chinese drug mixture weight 75% ethyl alcohol 45 DEG C refluxing extraction 2 hours, filter out medical fluid；
Step 3: again be added 8 times of Traditional Chinese drug mixture weight 75% ethyl alcohol 45 DEG C refluxing extraction 2 hours, filter out medical fluid；
Step 4: merging the medical fluid of step 2 and step 3, being concentrated into relative to the density of water is 0.6；
Step 5: by butanediol: after water=2:1 ratio mixing, the medical fluid of step 4 is added, puts to room temperature and filters, be warming up to 45 DEG C, 2 parts of p-aminobenzoic acid, 1 part of palmitoyl tripeptide 3 and 0.5 part of vitamin B6 is added, stirs evenly.
The hair restorer includes the component of following parts by weight:
Fat mesenchymal stem cell conditioned medium, fibroblast excretion body, 10 portions of mannitol, 0.5 portion of trehalose and 0.4 part Dextran 40.
The hair restorer the preparation method is as follows:
Step 1: preparing fat mesenchymal stem cell conditioned medium
1. the collection of fat mesenchymal stem cell conditioned medium: with 20mJ/cm2UVB irradiation dose irradiate 5 fat subsitutes between fill Matter stem cell, the fat mesenchymal stem cell after irradiation continue to cultivate, and collect the fat mesenchymal stem cell condition training of culture 48h Nutrient solution is stored in 4 DEG C after centrifugation；
2. the removal of impurities of fat mesenchymal stem cell conditioned medium is concentrated: being filled using ultrafiltration concentration technology between the fat being collected into Matter stem cell conditioned medium is cleaned, is once concentrated, secondary concentration, is obtained secondary concentration liquid and is filled to get between the fat Matter stem cell conditioned medium.
Step 2: preparation fibroblast excretion body
1. cultivate adherent fibroblast to degrees of fusion 90%, culture solution supernatant is collected, and be extracted into using supercentrifugation Fibrocyte excretion body；
2. passing on after the cell dissociation retained, and continue to cultivate, collect supernatant, extracts fibroblast with supercentrifugation later Excretion body, repeatedly to get the fibroblast excretion body.
Step 3: the fat mesenchymal stem cell that step 1 obtains is added in the fibroblast excretion body that step 2 obtains It in conditioned medium, stirs evenly, degerming is filtered using miillpore filter；
Step 4: carrying out total protein concentration measurement, total protein concentration 0.5-2mg/ to step 3 obtained material using BCA method mL；
Step 5: carrying out pH value measurement, pH value 6.5-8 to step 3 obtained material using pH meter.
Step 6: 10 portions of mannitol, 0.5 portion of trehalose and 0.4 part of Dextran 40 are added in step 3 obtained material Mixture, after packing freeze-drying to get the hair restorer, storage at normal temperature is spare.
Embodiment 10
The application method of nutritional agents of the present invention is as follows:
Step 1: the preparation of nutritional agents: preparing nutritional agents of the present invention；
Step 2: alopecia position sterilizes: first being cleaned with physiological saline to alopecia position, then carried out with Iodophor to alopecia position Disinfection；
Step 3: micropin imports: nutritional agents uniformly being dripped in alopecia position, carry out import operation, micropin using micropin introducing apparatus Importing process needs 40min；
Step 4: massage promote seep: after the completion of micropin import operation, the nutritional agents of alopecia site residues reuse beauty radiofrequency generator into Row massage, which promotees to seep to nutritional agents, to be fully absorbed.
Embodiment 11
The application method of hair restorer of the present invention is as follows:
Step 1: the preparation of hair restorer: preparing hair restorer of the present invention；
Step 2: alopecia position sterilizes: first being cleaned with physiological saline to alopecia position, then carried out with Iodophor to alopecia position Disinfection；
Step 3: with physiological saline solution hair restorer of the present invention；
Step 4: micropin imports: the hair restorer that step 3 dissolves uniformly being dripped in alopecia position, is led using micropin introducing apparatus Enter operation, micropin importing process needs 40min；
Step 5: massage promote seep: after the completion of micropin import operation, the hair restorer of alopecia site residues reuse beauty radiofrequency generator into Row massage, which promotees to seep to hair restorer, to be fully absorbed.
Embodiment 12
The application method of hair growth composition of the present invention is as follows:
Step 1: the preparation of hair growth composition: nutritional agents of the present invention and hair restorer of the present invention are prepared, by one 2mL nutritional agents is added in 0.6mg hair restorer, and sufficiently dissolution mixes, and hair growth composition is made；
Step 2: alopecia position sterilizes: first being cleaned with physiological saline to alopecia position, then carried out with Iodophor to alopecia position Disinfection；
Step 3: micropin imports: hair growth composition is uniformly dripped in alopecia position, carries out import operation using micropin introducing apparatus, Micropin importing process needs 40min；
Step 4: massage promotees to seep: after the completion of micropin import operation, the hair growth composition of alopecia site residues reuses beauty radio frequency Instrument carries out massage rush infiltration to hair growth composition and fully absorbs.
Comparative example 1
Beneficial effect in order to further illustrate the present invention provides comparative example 1 and compares with embodiment 3, the difference of the comparative example 1 It is: peppery peppermint of the invention is replaced with into ginger.
Comparative example 1 provides a kind of nutritional agents, and the nutritional agents includes the component of following parts by weight:
5 parts of ginsengs, 6 portions of cacumen biotaes, 4 parts of Radix Salviae Miltiorrhizaes, 7 parts of kuh-sengs, 2 portions of gingers, 2 parts of p-aminobenzoic acid, 1 part of palmitoyl tripeptide 3 and 0.5 Part vitamin B6.
1 nutritional agents of comparative example the preparation method is as follows:
Step 1: being put into extractor after 5 parts of ginsengs, 6 portions of cacumen biotaes, 4 parts of Radix Salviae Miltiorrhizaes, 7 parts of kuh-sengs, 2 portions of gingers are mixed, obtain Traditional Chinese drug mixture；
Step 2: be added 8 times of Traditional Chinese drug mixture weight 75% ethyl alcohol 45 DEG C refluxing extraction 2 hours, filter out medical fluid；
Step 3: again be added 8 times of Traditional Chinese drug mixture weight 75% ethyl alcohol 45 DEG C refluxing extraction 2 hours, filter out medical fluid；
Step 4: merging the medical fluid of step 2 and step 3, being concentrated into relative to the density of water is 0.6；
Step 5: by butanediol: after water=2:1 ratio mixing, the medical fluid of step 4 is added, puts to room temperature and filters, be warming up to 45 DEG C, 2 parts of p-aminobenzoic acid, 1 part of palmitoyl tripeptide 3 and 0.5 part of vitamin B6 is added, stirs evenly.
Comparative example 2
Beneficial effect in order to further illustrate the present invention provides comparative example 2 and compares with embodiment 3, the difference of the comparative example 2 It is: the parts by weight of p-aminobenzoic acid of the present invention is replaced with 6 parts.
Comparative example 2 provides a kind of nutritional agents, and the nutritional agents includes the component of following parts by weight:
5 parts of ginsengs, 6 portions of cacumen biotaes, 4 parts of Radix Salviae Miltiorrhizaes, 7 parts of kuh-sengs, 2 parts of peppery peppermints, 6 parts of p-aminobenzoic acid, 1 part of palmitoyl tripeptide 3 and 0.5 part of vitamin B6.
2 nutritional agents of comparative example the preparation method is as follows:
Step 1: being put into extractor after 5 parts of ginsengs, 6 portions of cacumen biotaes, 4 parts of Radix Salviae Miltiorrhizaes, 7 parts of kuh-sengs, 2 parts of peppery peppermints are mixed, obtain To Traditional Chinese drug mixture；
Step 2: be added 8 times of Traditional Chinese drug mixture weight 75% ethyl alcohol 45 DEG C refluxing extraction 2 hours, filter out medical fluid；
Step 3: again be added 8 times of Traditional Chinese drug mixture weight 75% ethyl alcohol 45 DEG C refluxing extraction 2 hours, filter out medical fluid；
Step 4: merging the medical fluid of step 2 and step 3, being concentrated into relative to the density of water is 0.6；
Step 5: by butanediol: after water=2:1 ratio mixing, the medical fluid of step 4 is added, puts to room temperature and filters, be warming up to 45 DEG C, 6 parts of p-aminobenzoic acid, 1 part of palmitoyl tripeptide 3 and 0.5 part of vitamin B6 is added, stirs evenly.
Comparative example 3
Beneficial effect in order to further illustrate the present invention provides comparative example 3 and compares with embodiment 6, the difference of the comparative example 3 It is: trehalose of the invention is replaced with into sucrose.
Comparative example 3 provides a kind of hair restorer, and the hair restorer includes the component of following parts by weight:
Fat mesenchymal stem cell conditioned medium, fibroblast excretion body, 12 portions of mannitol, 0.5 portion of sucrose and 0.4 part of right side Revolve sugared acid anhydride 40.
3 hair restorer of comparative example the preparation method is as follows:
Step 1: preparing fat mesenchymal stem cell conditioned medium
1. the collection of fat mesenchymal stem cell conditioned medium: with 20mJ/cm2UVB irradiation dose irradiate 5 fat subsitutes between fill Matter stem cell, the fat mesenchymal stem cell after irradiation continue to cultivate, and collect the fat mesenchymal stem cell condition training of culture 48h Nutrient solution is stored in 4 DEG C after centrifugation；
2. the removal of impurities of fat mesenchymal stem cell conditioned medium is concentrated: being filled using ultrafiltration concentration technology between the fat being collected into Matter stem cell conditioned medium is cleaned, is once concentrated, secondary concentration, is obtained secondary concentration liquid and is filled to get between the fat Matter stem cell conditioned medium.
Step 2: preparation fibroblast excretion body
1. cultivate adherent fibroblast to degrees of fusion 90%, culture solution supernatant is collected, and be extracted into using supercentrifugation Fibrocyte excretion body；
2. passing on after the cell dissociation retained, and continue to cultivate, collect supernatant, extracts fibroblast with supercentrifugation later Excretion body, repeatedly to get the fibroblast excretion body.
Step 3: the fat mesenchymal stem cell that step 1 obtains is added in the fibroblast excretion body that step 2 obtains It in conditioned medium, stirs evenly, degerming is filtered using miillpore filter；
Step 4: carrying out total protein concentration measurement, total protein concentration 0.5-2mg/ to step 3 obtained material using BCA method mL；
Step 5: carrying out pH value measurement, pH value 6.5-8 to step 3 obtained material using pH meter.
Step 6: 12 portions of mannitol, 0.5 portion of sucrose and 0.4 part of Dextran 40 are added in step 3 obtained material Mixture, to get the hair restorer, storage at normal temperature is spare for freeze-drying after packing.
Comparative example 4
Beneficial effect in order to further illustrate the present invention provides comparative example 4 and compares with embodiment 6, the difference of the comparative example 4 It is: the parts by weight of mannitol of the present invention is replaced with 15 parts.
Comparative example 4 provides a kind of hair restorer, and the hair restorer includes the component of following parts by weight:
Fat mesenchymal stem cell conditioned medium, fibroblast excretion body, 15 portions of mannitol, 0.5 portion of trehalose and 0.4 part Dextran 40.
4 hair restorer of comparative example the preparation method is as follows:
Step 1: preparing fat mesenchymal stem cell conditioned medium
1. the collection of fat mesenchymal stem cell conditioned medium: with 20mJ/cm2UVB irradiation dose irradiate 5 fat subsitutes between fill Matter stem cell, the fat mesenchymal stem cell after irradiation continue to cultivate, and collect the fat mesenchymal stem cell condition training of culture 48h Nutrient solution is stored in 4 DEG C after centrifugation；
2. the removal of impurities of fat mesenchymal stem cell conditioned medium is concentrated: being filled using ultrafiltration concentration technology between the fat being collected into Matter stem cell conditioned medium is cleaned, is once concentrated, secondary concentration, is obtained secondary concentration liquid and is filled to get between the fat Matter stem cell conditioned medium.
Step 2: preparation fibroblast excretion body
1. cultivate adherent fibroblast to degrees of fusion 90%, culture solution supernatant is collected, and be extracted into using supercentrifugation Fibrocyte excretion body；
2. passing on after the cell dissociation retained, and continue to cultivate, collect supernatant, extracts fibroblast with supercentrifugation later Excretion body, repeatedly to get the fibroblast excretion body.
Step 3: the fat mesenchymal stem cell that step 1 obtains is added in the fibroblast excretion body that step 2 obtains It in conditioned medium, stirs evenly, degerming is filtered using miillpore filter；
Step 4: carrying out total protein concentration measurement, total protein concentration 0.5-2mg/ to step 3 obtained material using BCA method mL；
Step 5: carrying out pH value measurement, pH value 6.5-8 to step 3 obtained material using pH meter.
Step 6: 15 portions of mannitol, 0.5 portion of trehalose and 0.4 part of Dextran 40 are added in step 3 obtained material Mixture, after packing freeze-drying to get the hair restorer, storage at normal temperature is spare.
Comparative example 5
Beneficial effect in order to further illustrate the present invention provides comparative example 5 and compares with embodiment 9, the difference of the comparative example 5 It is: the peppery peppermint in nutritional agents of the present invention is replaced with into ginger.
Comparative example 5 provides a kind of hair growth composition, and the hair growth composition includes nutritional agents and hair restorer, wherein
The nutritional agents includes the component of following parts by weight:
5 parts of ginsengs, 6 portions of cacumen biotaes, 4 parts of Radix Salviae Miltiorrhizaes, 7 parts of kuh-sengs, 2 portions of gingers, 2 parts of p-aminobenzoic acid, 1 part of palmitoyl tripeptide 3 and 0.5 Part vitamin B6.
The nutritional agents the preparation method is as follows:
Step 1: being put into extractor after 5 parts of ginsengs, 6 portions of cacumen biotaes, 4 parts of Radix Salviae Miltiorrhizaes, 7 parts of kuh-sengs, 2 portions of gingers are mixed, obtain Traditional Chinese drug mixture；
Step 2: be added 8 times of Traditional Chinese drug mixture weight 75% ethyl alcohol 45 DEG C refluxing extraction 2 hours, filter out medical fluid；
Step 3: again be added 8 times of Traditional Chinese drug mixture weight 75% ethyl alcohol 45 DEG C refluxing extraction 2 hours, filter out medical fluid；
Step 4: merging the medical fluid of step 2 and step 3, being concentrated into relative to the density of water is 0.6；
Step 5: by butanediol: after water=2:1 ratio mixing, the medical fluid of step 4 is added, puts to room temperature and filters, be warming up to 45 DEG C, 2 parts of p-aminobenzoic acid, 1 part of palmitoyl tripeptide 3 and 0.5 part of vitamin B6 is added, stirs evenly.
The hair restorer includes the component of following parts by weight:
Fat mesenchymal stem cell conditioned medium, fibroblast excretion body, 10 portions of mannitol, 0.5 portion of trehalose and 0.4 part Dextran 40.
The hair restorer the preparation method is as follows:
Step 1: preparing fat mesenchymal stem cell conditioned medium
1. the collection of fat mesenchymal stem cell conditioned medium: with 20mJ/cm2UVB irradiation dose irradiate 5 fat subsitutes between fill Matter stem cell, the fat mesenchymal stem cell after irradiation continue to cultivate, and collect the fat mesenchymal stem cell condition training of culture 48h Nutrient solution is stored in 4 DEG C after centrifugation；
2. the removal of impurities of fat mesenchymal stem cell conditioned medium is concentrated: being filled using ultrafiltration concentration technology between the fat being collected into Matter stem cell conditioned medium is cleaned, is once concentrated, secondary concentration, is obtained secondary concentration liquid and is filled to get between the fat Matter stem cell conditioned medium.
Step 2: preparation fibroblast excretion body
1. cultivate adherent fibroblast to degrees of fusion 90%, culture solution supernatant is collected, and be extracted into using supercentrifugation Fibrocyte excretion body；
2. passing on after the cell dissociation retained, and continue to cultivate, collect supernatant, extracts fibroblast with supercentrifugation later Excretion body, repeatedly to get the fibroblast excretion body.
Step 3: the fat mesenchymal stem cell that step 1 obtains is added in the fibroblast excretion body that step 2 obtains It in conditioned medium, stirs evenly, degerming is filtered using miillpore filter；
Step 4: carrying out total protein concentration measurement, total protein concentration 0.5-2mg/ to step 3 obtained material using BCA method mL；
Step 5: carrying out pH value measurement, pH value 6.5-8 to step 3 obtained material using pH meter.
Step 6: 10 portions of mannitol, 0.5 portion of trehalose and 0.4 part of Dextran 40 are added in step 3 obtained material Mixture, after packing freeze-drying to get the hair restorer, storage at normal temperature is spare.
Comparative example 6
Beneficial effect in order to further illustrate the present invention provides comparative example 6 and compares with embodiment 9, the difference of the comparative example 6 It is: the parts by weight of mannitol in hair restorer of the present invention is adjusted to 15 parts.
Comparative example 6 provides a kind of hair growth composition, and the hair growth composition includes nutritional agents and hair restorer, wherein
The nutritional agents includes the component of following parts by weight:
5 parts of ginsengs, 6 portions of cacumen biotaes, 4 parts of Radix Salviae Miltiorrhizaes, 7 parts of kuh-sengs, 2 parts of peppery peppermints, 2 parts of p-aminobenzoic acid, 1 part of palmitoyl tripeptide 3 and 0.5 part of vitamin B6.
The nutritional agents the preparation method is as follows:
Step 1: being put into extractor after 5 parts of ginsengs, 6 portions of cacumen biotaes, 4 parts of Radix Salviae Miltiorrhizaes, 7 parts of kuh-sengs, 2 parts of peppery peppermints are mixed, obtain To Traditional Chinese drug mixture；
Step 2: be added 8 times of Traditional Chinese drug mixture weight 75% ethyl alcohol 45 DEG C refluxing extraction 2 hours, filter out medical fluid；
Step 3: again be added 8 times of Traditional Chinese drug mixture weight 75% ethyl alcohol 45 DEG C refluxing extraction 2 hours, filter out medical fluid；
Step 4: merging the medical fluid of step 2 and step 3, being concentrated into relative to the density of water is 0.6；
Step 5: by butanediol: after water=2:1 ratio mixing, the medical fluid of step 4 is added, puts to room temperature and filters, be warming up to 45 DEG C, 2 parts of p-aminobenzoic acid, 1 part of palmitoyl tripeptide 3 and 0.5 part of vitamin B6 is added, stirs evenly.
The hair restorer includes the component of following parts by weight:
Fat mesenchymal stem cell conditioned medium, fibroblast excretion body, 15 portions of mannitol, 0.5 portion of trehalose and 0.4 part Dextran 40.
The hair restorer the preparation method is as follows:
Step 1: preparing fat mesenchymal stem cell conditioned medium
1. the collection of fat mesenchymal stem cell conditioned medium: with 20mJ/cm2UVB irradiation dose irradiate 5 fat subsitutes between fill Matter stem cell, the fat mesenchymal stem cell after irradiation continue to cultivate, and collect the fat mesenchymal stem cell condition training of culture 48h Nutrient solution is stored in 4 DEG C after centrifugation；
2. the removal of impurities of fat mesenchymal stem cell conditioned medium is concentrated: being filled using ultrafiltration concentration technology between the fat being collected into Matter stem cell conditioned medium is cleaned, is once concentrated, secondary concentration, is obtained secondary concentration liquid and is filled to get between the fat Matter stem cell conditioned medium.
Step 2: preparation fibroblast excretion body
1. cultivate adherent fibroblast to degrees of fusion 90%, culture solution supernatant is collected, and be extracted into using supercentrifugation Fibrocyte excretion body；
2. passing on after the cell dissociation retained, and continue to cultivate, collect supernatant, extracts fibroblast with supercentrifugation later Excretion body, repeatedly to get the fibroblast excretion body.
Step 3: the fat mesenchymal stem cell that step 1 obtains is added in the fibroblast excretion body that step 2 obtains It in conditioned medium, stirs evenly, degerming is filtered using miillpore filter；
Step 4: carrying out total protein concentration measurement, total protein concentration 0.5-2mg/ to step 3 obtained material using BCA method mL；
Step 5: carrying out pH value measurement, pH value 6.5-8 to step 3 obtained material using pH meter.
Step 6: 15 portions of mannitol, 0.5 portion of trehalose and 0.4 part of Dextran 40 are added in step 3 obtained material Mixture, after packing freeze-drying to get the hair restorer, storage at normal temperature is spare.
Comparative example 7
Beneficial effect in order to further illustrate the present invention provides a kind of Chinese medicine compound prescription hair restorer, which is Using the method preparation in patent CN102727595A.
Experiment 1: hair-growing effects verifying
Test specimen:
The nutritional agents of embodiment 1-3 preparation, the hair restorer of embodiment 4-6 preparation, embodiment 7-9 preparation hair growth composition, right The nutritional agents of ratio 1-2 preparation, the hair restorer of comparative example 3-4 preparation, the hair growth composition of comparative example 5-6 preparation, comparative example 7 are made Standby Chinese medicine compound prescription hair restorer.
Test method:
The nutritional agents of embodiment 1-3, comparative example 1-2 preparation is tested according to the application method of embodiment 10；Embodiment 4-6, The hair restorer of comparative example 3-4 preparation is tested according to the application method of embodiment 11；Embodiment 7-9, comparative example 5-6 are according to reality The application method for applying example 12 is tested；Comparative example 7 is tested according to the method in patent CN102727595A；By there is experience Operator carry out the effect of testing experiment sample.
Alopecia efficacy evaluation:
Cure: the new hair in alopecia position is all grown, and restores normal；
Improve: alopecia stops, and the new hair portion in alopecia position is grown；
It is not cured: continuing alopecia, new hair is not grown.
Compliance test result:
Be androgenetic alopecia to clinical diagnosis, can by doctor's advice partner treatment person, the age in 16-45 one full year of life patient 480, at random It is divided into 16 groups of A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, every group of 30 people.Wherein,
A group uses the nutritional agents prepared by the above embodiment of the present invention 1,
B group uses the nutritional agents prepared by the above embodiment of the present invention 2,
C group uses the nutritional agents prepared by the above embodiment of the present invention 3,
D group uses the hair restorer prepared by the above embodiment of the present invention 4,
E group uses the hair restorer prepared by the above embodiment of the present invention 5,
F group uses the hair restorer prepared by the above embodiment of the present invention 6,
G group uses the hair growth composition prepared by the above embodiment of the present invention 7,
H group uses the hair growth composition prepared by the above embodiment of the present invention 8,
I group uses the hair growth composition prepared by the above embodiment of the present invention 9,
J group uses the nutritional agents prepared by comparative example 1,
K group uses the nutritional agents prepared by comparative example 2,
L group uses the hair restorer prepared by comparative example 3,
M group uses the hair restorer prepared by comparative example 4,
N group uses the hair growth composition prepared by comparative example 5,
O group uses the hair growth composition prepared by comparative example 6,
P group uses the Chinese medicine compound prescription hair restorer prepared by comparative example 7,
Test result:
Nutritional agents prepared by the embodiment of the present invention 1 treats androgenetic alopecia patient 30, cures 23, improves 5, and not more 2 Example, total effective rate 82%, and any toxic side effect and non-stimulated no allergic phenomena are had no during treating.
Nutritional agents prepared by the embodiment of the present invention 2 treats androgenetic alopecia patient 30, cures 24, improves 3, not More 3, total effective rate 81%, and any toxic side effect and non-stimulated no allergic phenomena are had no during treating.
Nutritional agents prepared by the embodiment of the present invention 3 treats androgenetic alopecia patient 30, cures 26, improves 3, not More 1, total effective rate 88%, and any toxic side effect and non-stimulated no allergic phenomena are had no during treating.
Nutritional agents prepared by comparative example 1 treats androgenetic alopecia patient 30, cures 16, improves 3, and not more 11 Example, total effective rate 80%.
Nutritional agents prepared by comparative example 2 treats androgenetic alopecia patient 30, cures 14, improves 4, and not more 12 Example, total effective rate 90%, while patient has dandruff appearance and irritating allergic phenomena during treatment.
Hair restorer prepared by the embodiment of the present invention 4 treats androgenetic alopecia patient 30, cures 22, improves 6, not More 2, total effective rate 89%, and any toxic side effect and non-stimulated no allergic phenomena are had no during treating.
Hair restorer prepared by the embodiment of the present invention 5 treats androgenetic alopecia patient 30, cures 23, improves 4, not More 3, total effective rate 87%, and any toxic side effect and non-stimulated no allergic phenomena are had no during treating.
Hair restorer prepared by the embodiment of the present invention 6 treats androgenetic alopecia patient 30, cures 25, improves 4, not More 1, total effective rate 95%, and any toxic side effect and non-stimulated no allergic phenomena are had no during treating.
Hair restorer prepared by comparative example 3 treats androgenetic alopecia patient 30, cures 13, improves 5, and not more 12 Example, total effective rate 82%.
Hair restorer prepared by comparative example 4 treats androgenetic alopecia patient 30, cures 12, improves 5, and not more 13 Example, total effective rate 81%.
Hair growth composition prepared by the embodiment of the present invention 7 treats androgenetic alopecia patient 30, cures 27, improvement 2 Example, not more 1, total effective rate 97%, and any toxic side effect and non-stimulated no allergic phenomena are had no during treating.
Hair growth composition prepared by the embodiment of the present invention 8 treats androgenetic alopecia patient 30, cures 26, improvement 2 Example, not more 2, total effective rate 96%, and any toxic side effect and non-stimulated no allergic phenomena are had no during treating.
Hair growth composition prepared by the embodiment of the present invention 9 treats androgenetic alopecia patient 30, cures 28, improvement 2 Example, total effective rate 100%, and any toxic side effect and non-stimulated no allergic phenomena are had no during treating.
Hair growth composition prepared by comparative example 5 treats androgenetic alopecia patient 30, cures 18, improves 4, is not cured 8, total effective rate 73%.
Hair growth composition prepared by comparative example 6 treats androgenetic alopecia patient 30, cures 20, improves 3, is not cured 7, total effective rate 77%.
Chinese medicine compound prescription hair restorer prepared by comparative example 7 treats androgenetic alopecia patient 30, cures 21, improves 2, It is not cured 7, total effective rate 80%.
Typical case and curative effect:
Patient Zhang, male, 31 years old, because of great work intensity, is frequently on night shift, and apparent M type hair loss area, alopecia etc. is presented in forehead Grade III level alopecia duration 5 years, was once treated once, and without any effect, using nutritional agents of the present invention, one week 1 time importing is controlled The area forehead M has a large amount of hairs to generate after treating 3 months.
Patient Lee, female, 40 years old, typical female androgen alopecia, hair sparse at forehead and crown clinking, alopecia It duration 3 years, did not treated, but carried out hair-waving before, hair-waving causes its hair quality impaired serious, and scalp is also had damage, rear to use Hair restorer of the present invention, one week 1 time importing, hair-growing effects are obvious after 3 months, there is natural on-off cycles of hair growth at hair sparse.
Patient cares for certain, and male, 31 years old, alopecia 2-3, chronic stress was big, sleeps bad, never carries out since work is busy Any treatment, alopecia grade are male II grades, using hair growth composition of the present invention, one week 1 time importing, hair tonic after treatment 2 months Effect is obvious.
It should be understood that the present invention disclosed is not limited only to specific method, scheme and the substance of description, because these It is alterable.It will also be understood that purpose of the terminology used here just for the sake of the specific embodiment scheme of description, rather than It is intended to limit the scope of the invention, the scope of the present invention is limited solely by the attached claims.
Those skilled in the art, which will also be appreciated that or be able to confirm that, uses no more than routine experiment, institute herein The many equivalents for the specific embodiment of the invention stated.These equivalents are also contained in the attached claims.

Claims (10)

1. a kind of hair restorer, the hair restorer includes fat mesenchymal stem cell conditioned medium, fibroblast excretion body, sweet Reveal alcohol, trehalose and Dextran 40, wherein the fat mesenchymal stem cell is used with 20-100mJ/cm2UVB irradiation Dose delivery.
2. hair restorer according to claim 1, wherein the parts by weight of the mannitol are 10-14,
The parts by weight of the trehalose are 0.5-0.6, and the parts by weight of the Dextran 40 are 0.4-0.5.
3. a kind of preparation method of hair restorer, wherein the method comprises the steps of:
Step 1: preparing fat mesenchymal stem cell conditioned medium
1. the collection of fat mesenchymal stem cell conditioned medium: with 20-100mJ/cm2UVB irradiation dose irradiation 3-10 for rouge Fat mescenchymal stem cell, the fat mesenchymal stem cell after irradiation continue to cultivate, and collect the fat mesenchymal cultivated 48-72 hours Stem cell conditioned medium；
2. the removal of impurities of fat mesenchymal stem cell conditioned medium is concentrated: being filled using ultrafiltration concentration technology between the fat being collected into Matter stem cell conditioned medium is cleaned, is once concentrated, secondary concentration, is obtained secondary concentration liquid and is filled to get between the fat Matter stem cell conditioned medium；
Step 2: preparation fibroblast excretion body
1. cultivate adherent fibroblast to degrees of fusion 90%, culture solution supernatant is collected, and be extracted into using supercentrifugation Fibrocyte excretion body；
2. passing on after the cell dissociation retained, and continue to cultivate, collect supernatant, extracts fibroblast with supercentrifugation later Excretion body is to get the fibroblast excretion body；
Step 3: the fat mesenchymal stem cell condition that step 1 obtains is added in the fibroblast excretion body that step 2 obtains It in culture solution, stirs evenly, filtration sterilization；
Step 4: the mixture of mannitol, trehalose and Dextran 40 is added in step 3 obtained material, freezed after packing Drying is to get the hair restorer.
4. according to the method described in claim 3, wherein, the parts by weight of the mannitol are 10-14, the weight of the trehalose Amount number is 0.5-0.6, and the parts by weight of the Dextran 40 are 0.4-0.5.
5. according to the method described in claim 3, wherein, the hair restorer is the described in any item hair restorers of claim 1-2.
6. a kind of hair growth composition, the composition include safe and effective amount nutritional agents and safe and effective amount such as claim The described in any item hair restorers of 1-2, wherein the nutritional agents include ginseng, cacumen biotae, Radix Salviae Miltiorrhizae, kuh-seng, peppery peppermint, to amino Benzoic acid, palmitoyl tripeptide 3 and vitamin B6.
7. composition according to claim 6, wherein according to parts by weight, the nutritional agents includes:
2-7 parts of ginsengs, 3-8 portions of cacumen biotaes, 2-7 parts of Radix Salviae Miltiorrhizaes, 5-10 parts of kuh-sengs, 1-6 parts of peppery peppermints, 1-5 parts of p-aminobenzoic acid, 0.5-2 parts of palmitoyl tripeptide 3s and 0.1-2 parts of vitamin B6s.
8. composition according to claim 7, wherein according to parts by weight, the nutritional agents includes:
5 parts of ginsengs, 6 portions of cacumen biotaes, 4 parts of Radix Salviae Miltiorrhizaes, 7 parts of kuh-sengs, 2 parts of peppery peppermints, 2 parts of p-aminobenzoic acid, 1 part of palmitoyl tripeptide 3 and 0.5 part of vitamin B6.
9. composition according to claim 6, wherein the nutritional agents the preparation method is as follows:
Step 1: being put into extractor after ginseng, cacumen biotae, Radix Salviae Miltiorrhizae, kuh-seng and peppery peppermint are mixed, Traditional Chinese drug mixture is obtained；
Step 2: be added 5-10 times of Traditional Chinese drug mixture weight 75% ethyl alcohol 40-48 DEG C refluxing extraction 1.5-2.5 hours, filter out Medical fluid；
Step 3: again be added 5-10 times of Traditional Chinese drug mixture weight 75% ethyl alcohol 40-48 DEG C refluxing extraction 1.5-2.5 hours, Filter out medical fluid；
Step 4: merging the medical fluid of step 2 and step 3, being concentrated into relative to the density of water is 0.6-0.9；
Step 5: by butanediol: after water=1-3:1 ratio mixing, the medical fluid of step 4 is added, puts to room temperature and filters, be warming up to 43-48 DEG C, p-aminobenzoic acid, palmitoyl tripeptide 3 and vitamin B6 is added, stirs evenly.
10. the described in any item hair restorers of claim 1-2 are preparing the purposes in hair growth composition or pharmaceutical composition.
CN201910640960.7A 2019-07-16 2019-07-16 Hair restorer Withdrawn CN110339338A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110964689A (en) * 2019-11-27 2020-04-07 浙江卫未生物医药科技有限公司 Method for restoring dryness or proliferation capacity of hair follicle source cells
CN111643436A (en) * 2020-07-16 2020-09-11 陕西中鸿科瑞再生医学研究院有限公司 Pilatory containing sponginum spicules
CN114699446A (en) * 2022-04-29 2022-07-05 诺赛联合(北京)生物医学科技有限公司 Exosome compound liquid for treating alopecia and preparation method thereof
CN114917250A (en) * 2022-05-06 2022-08-19 广东发亿科技有限公司 Application of fibroblast outer vesicles hFB-EVs in alopecia treatment product
WO2022251277A1 (en) * 2021-05-26 2022-12-01 Figene, Llc Methods and compositions for alopecia treatment using fibroblasts and fibroblast-derived products

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110964689A (en) * 2019-11-27 2020-04-07 浙江卫未生物医药科技有限公司 Method for restoring dryness or proliferation capacity of hair follicle source cells
CN111643436A (en) * 2020-07-16 2020-09-11 陕西中鸿科瑞再生医学研究院有限公司 Pilatory containing sponginum spicules
WO2022251277A1 (en) * 2021-05-26 2022-12-01 Figene, Llc Methods and compositions for alopecia treatment using fibroblasts and fibroblast-derived products
CN114699446A (en) * 2022-04-29 2022-07-05 诺赛联合(北京)生物医学科技有限公司 Exosome compound liquid for treating alopecia and preparation method thereof
CN114917250A (en) * 2022-05-06 2022-08-19 广东发亿科技有限公司 Application of fibroblast outer vesicles hFB-EVs in alopecia treatment product

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Application publication date: 20191018