CN110317837A - 用于在有效降低水用量的低磷酸盐培养基中发酵含有co的气态底物的方法 - Google Patents
用于在有效降低水用量的低磷酸盐培养基中发酵含有co的气态底物的方法 Download PDFInfo
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- CN110317837A CN110317837A CN201910402394.6A CN201910402394A CN110317837A CN 110317837 A CN110317837 A CN 110317837A CN 201910402394 A CN201910402394 A CN 201910402394A CN 110317837 A CN110317837 A CN 110317837A
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- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
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Abstract
Description
事件编号 | 时间(小时) | 行为 |
1 | 0 | 培养基变为低磷酸盐培养基(2.92 mM H<sub>3</sub>PO<sub>4</sub>) |
2 | 59.82 | 培养基变为低磷酸盐培养基(4.38 mM H<sub>3</sub>PO<sub>4</sub>) |
3 | 108.74 | 培养基变为含有H<sub>2</sub>SO<sub>4</sub>的低磷酸盐培养基(2.93 mM H<sub>3</sub>PO<sub>4</sub>),开始以0.4 ml/分钟的182 mM NH<sub>4</sub>OH泵送。 |
4 | 135.32 | 碱溶液从7.7% NaHCO<sub>3</sub>变为182 mM NH<sub>4</sub>OH。NH<sub>4</sub>OH泵送溶液从182 mM变为92 mM |
5 | 135.41 | NH<sub>4</sub>OH泵送流速从0.3 ml/分钟提高至0.5 ml/分钟 |
6 | 158.42 | 培养基变为低磷酸盐培养基(2.92 mM H<sub>3</sub>PO<sub>4</sub>),无NH<sub>4</sub>Cl,无H<sub>2</sub>SO<sub>4</sub> |
7 | 158.47 | NH<sub>4</sub>泵送从0.6 ml/分钟降低至0.5 ml/分钟 |
8 | 181.49 | NH<sub>4</sub>泵送从0.5 ml/分钟降低至0.4 ml/分钟 |
9 | 224.74 | NH<sub>4</sub>泵送从0.4 ml/分钟降低至0.2 ml/分钟 |
10 | 250.09 | 碱溶液从182 mM变为364 mM |
11 | 255.86 | NH<sub>4</sub>泵送停止 |
12 | 261.61 | 加入5 mL 0.5M NH<sub>4</sub>溶液 |
13 | 264.74 | 以0.2 ml/分钟再次开始NH<sub>4</sub>泵送 |
14 | 270.57 | NH<sub>4</sub>泵送停止 |
15 | 279.32 | 维生素浓度从0.5 ml/L提高至1 ml/L |
16 | 280.66 | 碱溶液从364 mM变为0.5M |
17 | 392.22 | 降低的渗透物流量,以降低细胞密度。目标3 g/L |
18 | 441.82 | 维生素浓度从1.0 ml/L提高至1.6 ml/L |
19 | 489.07 | 培养基变为具有作为干粉末加入的第一储液组分和以含水形式加入的第二储液的低磷酸盐培养基 |
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US14/293,111 US9885063B2 (en) | 2013-06-10 | 2014-06-02 | Process for fermenting co-containing gaseous substrates in a low phosphate medium effective for reducing water usage |
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CA2913196A1 (en) | 2014-12-18 |
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EP3008195A1 (en) | 2016-04-20 |
CN110317837B (zh) | 2024-04-02 |
WO2014200810A1 (en) | 2014-12-18 |
KR102214878B1 (ko) | 2021-02-10 |
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JP2016521574A (ja) | 2016-07-25 |
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US9885063B2 (en) | 2018-02-06 |
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KR20160018715A (ko) | 2016-02-17 |
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