CN110305928A - Digest the method and enzymolysis protein feedstuff of Amino acid fermentation bacteria - Google Patents
Digest the method and enzymolysis protein feedstuff of Amino acid fermentation bacteria Download PDFInfo
- Publication number
- CN110305928A CN110305928A CN201910553243.0A CN201910553243A CN110305928A CN 110305928 A CN110305928 A CN 110305928A CN 201910553243 A CN201910553243 A CN 201910553243A CN 110305928 A CN110305928 A CN 110305928A
- Authority
- CN
- China
- Prior art keywords
- amino acid
- enzymatic hydrolysis
- mycoprotein
- fermentation bacteria
- acid fermentation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 150000001413 amino acids Chemical class 0.000 title claims abstract description 103
- 238000000034 method Methods 0.000 title claims abstract description 42
- 241000894006 Bacteria Species 0.000 title claims abstract description 40
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 39
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 39
- 238000000855 fermentation Methods 0.000 title claims abstract description 35
- 230000004151 fermentation Effects 0.000 title claims abstract description 35
- 230000007071 enzymatic hydrolysis Effects 0.000 claims abstract description 55
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims abstract description 55
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 claims abstract description 35
- 102000004190 Enzymes Human genes 0.000 claims abstract description 32
- 108090000790 Enzymes Proteins 0.000 claims abstract description 32
- 239000000843 powder Substances 0.000 claims abstract description 23
- 230000009849 deactivation Effects 0.000 claims abstract description 21
- 239000000463 material Substances 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 238000000265 homogenisation Methods 0.000 claims abstract description 8
- 229940088598 enzyme Drugs 0.000 claims description 31
- 239000004365 Protease Substances 0.000 claims description 17
- 108090000526 Papain Proteins 0.000 claims description 13
- 229940055729 papain Drugs 0.000 claims description 13
- 235000019834 papain Nutrition 0.000 claims description 13
- 244000063299 Bacillus subtilis Species 0.000 claims description 9
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 9
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 8
- 239000002994 raw material Substances 0.000 claims description 8
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 claims description 7
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 claims description 7
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 claims description 7
- 230000007062 hydrolysis Effects 0.000 claims description 7
- 238000006460 hydrolysis reaction Methods 0.000 claims description 7
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 6
- 239000004472 Lysine Substances 0.000 claims description 6
- 108091005804 Peptidases Proteins 0.000 claims description 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 4
- 235000019419 proteases Nutrition 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 2
- 238000005507 spraying Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 235000001014 amino acid Nutrition 0.000 description 77
- 235000018102 proteins Nutrition 0.000 description 33
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 15
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 12
- 108090000765 processed proteins & peptides Proteins 0.000 description 6
- 238000001694 spray drying Methods 0.000 description 6
- 241000193830 Bacillus <bacterium> Species 0.000 description 5
- 235000013922 glutamic acid Nutrition 0.000 description 5
- 239000004220 glutamic acid Substances 0.000 description 5
- 238000002156 mixing Methods 0.000 description 5
- 102000057297 Pepsin A Human genes 0.000 description 4
- 108090000284 Pepsin A Proteins 0.000 description 4
- 235000019621 digestibility Nutrition 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 235000019629 palatability Nutrition 0.000 description 4
- 229940111202 pepsin Drugs 0.000 description 4
- 101710130006 Beta-glucanase Proteins 0.000 description 2
- 235000019750 Crude protein Nutrition 0.000 description 2
- 102000016943 Muramidase Human genes 0.000 description 2
- 108010014251 Muramidase Proteins 0.000 description 2
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229960000274 lysozyme Drugs 0.000 description 2
- 239000004325 lysozyme Substances 0.000 description 2
- 235000010335 lysozyme Nutrition 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- DWNBOPVKNPVNQG-LURJTMIESA-N (2s)-4-hydroxy-2-(propylamino)butanoic acid Chemical compound CCCN[C@H](C(O)=O)CCO DWNBOPVKNPVNQG-LURJTMIESA-N 0.000 description 1
- 108091005508 Acid proteases Proteins 0.000 description 1
- 244000189799 Asimina triloba Species 0.000 description 1
- 235000006264 Asimina triloba Nutrition 0.000 description 1
- 235000009467 Carica papaya Nutrition 0.000 description 1
- RGJOEKWQDUBAIZ-IBOSZNHHSA-N CoASH Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCS)O[C@H]1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-IBOSZNHHSA-N 0.000 description 1
- ACTIUHUUMQJHFO-UHFFFAOYSA-N Coenzym Q10 Natural products COC1=C(OC)C(=O)C(CC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UHFFFAOYSA-N 0.000 description 1
- 235000019733 Fish meal Nutrition 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000000433 anti-nutritional effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- RGJOEKWQDUBAIZ-UHFFFAOYSA-N coenzime A Natural products OC1C(OP(O)(O)=O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-UHFFFAOYSA-N 0.000 description 1
- 239000005516 coenzyme A Substances 0.000 description 1
- 235000017471 coenzyme Q10 Nutrition 0.000 description 1
- ACTIUHUUMQJHFO-UPTCCGCDSA-N coenzyme Q10 Chemical compound COC1=C(OC)C(=O)C(C\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UPTCCGCDSA-N 0.000 description 1
- 229940093530 coenzyme a Drugs 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- KDTSHFARGAKYJN-UHFFFAOYSA-N dephosphocoenzyme A Natural products OC1C(O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 KDTSHFARGAKYJN-UHFFFAOYSA-N 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 235000021050 feed intake Nutrition 0.000 description 1
- 239000004467 fishmeal Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000003595 mist Substances 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 239000011236 particulate material Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- NPCOQXAVBJJZBQ-UHFFFAOYSA-N reduced coenzyme Q9 Natural products COC1=C(O)C(C)=C(CC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)C)C(O)=C1OC NPCOQXAVBJJZBQ-UHFFFAOYSA-N 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000010025 steaming Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 229940035936 ubiquinone Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
Abstract
The present invention relates to a kind of methods and enzymolysis protein feedstuff for digesting Amino acid fermentation bacteria.The method of the enzymatic hydrolysis Amino acid fermentation bacteria is the following steps are included: taking amino acid mycoprotein and crushing and obtain amino acid mycoprotein powder, amino acid mycoprotein powder is mixed with water according to mass ratio for 1:2.5~1:4, broken homogenization is carried out later, obtains amino acid mycoprotein after broken homogeneous；Amino acid mycoprotein after broken homogeneous is taken, adjusting pH is neutrality, and 1~4% neutral proteinase that amino acid mycoprotein quality after broken homogeneous is added later carries out enzymatic hydrolysis 12~for 24 hours, enzyme deactivation is carried out after enzymatic hydrolysis, material after must digesting；Take enzymatic hydrolysis after material be spray-dried to get.Technique is relatively simple, and enzymatic hydrolysis is directly spray-dried after terminating, and reduces production cost, energy saving.
Description
Technical field
The invention belongs to enzymolysis protein technical fields, and in particular to a kind of method and enzymatic hydrolysis for digesting Amino acid fermentation bacteria
Protein feed.
Background technique
China is an amino acids production big country, wherein producing glutamic acid per year more than 1,000,000 tons, in amino acids production process
A large amount of byproduct, i.e. amino acid mycoprotein can be generated, single glu thalline protein yield has just reached every year in China according to statistics
More than 20 ten thousand tons, and also increasing year by year.Amino acid mycoprotein content is high, B group vitamin rich in, minerals, coenzyme
A, ubiquinone etc. is free of anti-nutritional factors and medicament residue, is very good protein feed resources, but amino acid mycoprotein
Due to the limitation of stoving process, protein solubility is extremely low, and Pepsin digestibility is low, and amino acid mycoprotein palatability
Difference influences animal feed intake, these disadvantages cause the utilization rate of amino acid mycoprotein limited.Based on background above using specific
Enzymolysis process amino acid mycoprotein is digested, improve amino acid mycoprotein palatability, improve amino acid thallus
The utilization rate of albumen.
Application publication number is that the patent of invention of CN102719510A discloses a kind of glutamic acid fermentation bacteria utilization method, should
Method is the following steps are included: (1) utilizes high-speed dish piece seperator by the glutamic acid feed liquid and thallus egg in glutamic acid fermentation bacterium solution
White to be separated, the revolving speed of high-speed dish piece machine separating thallus is 4000~5000r/min, by conditions above, by glutamic acid material
Liquid and mycoprotein separation.(2) complex enzyme pair is used after isolated mycoprotein being diluted with water to solid content 5%~10%
It is digested, and enzymolysis liquid, enzymatic hydrolysis condition are obtained are as follows: and 55 DEG C of temperature, pH6.5, time 6h, wherein complex enzyme are as follows: lysozyme, acid
Property protease, 1,4 beta-glucanase, proportions are lysozyme: acid protease: 1,4 beta-glucanase 2: 5: 1, complex enzyme addition
Amount are as follows: 5-7%；(3) to above-mentioned enzymolysis liquid using disc separator separation removal cell wall, gained supernatant is through the board-like steaming of triple effect
Device low-temperature evaporation is sent out, the evaporator one imitates 80 DEG C of feeding temperature <, and 45 DEG C of end effect drop temperature <, gained paste butt contains
Enzymatic hydrolysis mycoprotein cream is made up to 65%, directly packaging in amount.This method digest when using enzyme type it is more and digest after point
It is complex from treatment process.
Summary of the invention
The first purpose of the invention is to provide a kind of methods for digesting Amino acid fermentation bacteria to solve existing enzymatic hydrolysis
Method digest when using enzyme type it is more and digest after separating treatment the process is more complicated the problem of.
A second object of the present invention is to provide a kind of enzymolysis protein feedstuffs.
In order to achieve the goal above, the technical scheme adopted by the invention is as follows: the method for digesting Amino acid fermentation bacteria, including
Following steps:
1) pretreatment of raw material: taking amino acid mycoprotein and crushing obtains amino acid mycoprotein powder, by amino acid thallus
Albumen powder is that 1:2.5~1:4 is mixed according to mass ratio with water, carries out broken homogenization later, obtains amino after broken homogeneous
Sour mycoprotein；
2) it digests: taking amino acid mycoprotein after the broken homogeneous in step 1), adjusting pH is neutrality, is added later broken
1~4% neutral proteinase of amino acid mycoprotein quality carries out enzymatic hydrolysis 12~for 24 hours after homogeneous, goes out after enzymatic hydrolysis
Enzyme, material after must digesting；
3) dry: take after the enzymatic hydrolysis in step 2) material to be spray-dried to get.
Further, the pH of enzymatic hydrolysis described in step 2) is 7.0~8.0, and hydrolysis temperature is 40~60 DEG C, uses hydroxide
It is 7.0~8.0 that sodium solution, which reconciles the pH of amino acid mycoprotein after broken homogeneous,.
Further, the temperature of enzyme deactivation described in step 2 is 70~90 DEG C, and the enzyme deactivation time is 20~50min.
Further, neutral proteinase described in step 2) includes the raw neutral proteinase of producing bacillus subtilis or pawpaw
One or both of protease.
Further, in the neutral proteinase between producing bacillus subtilis raw neutral proteinase and papain
Mass ratio be 9:1~7:3.
Further, amino acid mycoprotein described in step 1) is glu thalline protein, tryptophan mycoprotein, relies
One of propylhomoserin mycoprotein, serine mycoprotein are a variety of.
Further, it is to be handled using high pressure homogenizer that homogeneous is crushed described in step 1), and homogeneous is in 170MPa
Under the conditions of homogeneous it is primary.
Further, 170 DEG C of the intake air temperature of spray drying described in step 3), 60 DEG C of air outlet temperature.
Further, 80 meshes are crossed after crushing described in step 1) up to the amino acid mycoprotein powder.
The enzymolysis protein feedstuff prepared using the method for above-mentioned enzymatic hydrolysis Amino acid fermentation bacteria.
Beneficial effects of the present invention:
The method of enzymatic hydrolysis Amino acid fermentation bacteria of the invention, technique is relatively simple, and enzymatic hydrolysis is directly sprayed after terminating
Mist is dry, reduces production cost, energy saving, and the enzymolysis protein feedstuff obtained after drying can be used directly.
The method of enzymatic hydrolysis Amino acid fermentation bacteria of the invention, the raw material used are feed particulate material, and nutritive value is lower,
Protein solubility is lower, and small peptide content is relatively low, by fermentation process, obtains the higher fermentation material of small peptide content, develops
New protein sources feedstuff alleviates the crisis of protein sources feedstuff, can be used for substituting fish meal.
The enzymolysis protein feedstuff of the method preparation of enzymatic hydrolysis Amino acid fermentation bacteria of the invention, palatability is preferable, and small peptide contains
Amount up to 50%, Pepsin digestibility is greater than 90%, and protein solubility is greater than 70%, greatly improves the utilization of albumen
Efficiency.
Specific embodiment
Below in conjunction with embodiment, the invention will be further described.
Embodiment 1
The method of the enzymatic hydrolysis Amino acid fermentation bacteria of the present embodiment, comprising the following steps:
1) pretreatment of raw material: taking amino acid mycoprotein, and amino acid mycoprotein includes glu thalline protein, tryptophan
Mycoprotein, lysine bacteria body protein, serine mycoprotein.Amino acid mycoprotein is subjected to powder using beater disintegrating machine
It is broken, 80 meshes are crossed after crushing up to amino acid mycoprotein powder.According to mass ratio it is 1 by amino acid mycoprotein powder and water:
2.5 mixing carry out broken homogenization using high pressure homogenizer later, and homogeneous is primary under the conditions of 170MPa, obtain broken equal
Amino acid mycoprotein after matter.
2) it digests: taking amino acid mycoprotein after the broken homogeneous in step 1), adjusted pH using sodium hydroxide solution
It is 7.0,1% neutral proteinase of amino acid mycoprotein quality after broken homogeneous is added later, digests 12h, hydrolysis temperature
It is 40 DEG C.Neutral proteinase includes the raw neutral proteinase and papain of producing bacillus subtilis, withered in neutral proteinase
The mass ratio between neutral proteinase and papain that careless bacillus generates is 9:1.Enzyme deactivation is carried out after enzymatic hydrolysis, is gone out
The temperature of enzyme is 70 DEG C, and the enzyme deactivation time is 20min.Material after must being digested after enzyme deactivation.
3) dry: take material after the enzymatic hydrolysis in step 2) to be spray-dried, 170 DEG C of the intake air temperature of spray drying,
60 DEG C of air outlet temperature to get.
The enzymolysis protein feedstuff of the present embodiment, i.e., using the present embodiment enzymatic hydrolysis Amino acid fermentation bacteria method prepare and
?.
Embodiment 2
The method of the enzymatic hydrolysis Amino acid fermentation bacteria of the present embodiment, comprising the following steps:
1) pretreatment of raw material: taking amino acid mycoprotein, and amino acid mycoprotein includes glu thalline protein, tryptophan
Mycoprotein, lysine bacteria body protein, serine mycoprotein.Amino acid mycoprotein is subjected to powder using beater disintegrating machine
It is broken, 80 meshes are crossed after crushing up to amino acid mycoprotein powder.It according to mass ratio is 1:4 by amino acid mycoprotein powder and water
Mixing carries out broken homogenization using high pressure homogenizer later, and homogeneous is primary under the conditions of 170MPa, after obtaining broken homogeneous
Amino acid mycoprotein.
2) it digests: taking amino acid mycoprotein after the broken homogeneous in step 1), adjusted pH using sodium hydroxide solution
It is 8.0,2% neutral proteinase of amino acid mycoprotein quality after broken homogeneous is added later, digests for 24 hours, hydrolysis temperature
It is 50 DEG C.Neutral proteinase includes the raw neutral proteinase and papain of producing bacillus subtilis, withered in neutral proteinase
The mass ratio between neutral proteinase and papain that careless bacillus generates is 9:3.Enzyme deactivation is carried out after enzymatic hydrolysis, is gone out
The temperature of enzyme is 80 DEG C, and the enzyme deactivation time is 30min.Material after must being digested after enzyme deactivation.
3) dry: take material after the enzymatic hydrolysis in step 2) to be spray-dried, 160 DEG C of the intake air temperature of spray drying,
65 DEG C of air outlet temperature to get.
The enzymolysis protein feedstuff of the present embodiment, i.e., using the present embodiment enzymatic hydrolysis Amino acid fermentation bacteria method prepare and
?.
Embodiment 3
The method of the enzymatic hydrolysis Amino acid fermentation bacteria of the present embodiment, comprising the following steps:
1) pretreatment of raw material: taking amino acid mycoprotein, and amino acid mycoprotein includes glu thalline protein, tryptophan
Mycoprotein, lysine bacteria body protein, serine mycoprotein.Amino acid mycoprotein is subjected to powder using beater disintegrating machine
It is broken, 80 meshes are crossed after crushing up to amino acid mycoprotein powder.It according to mass ratio is 1:3 by amino acid mycoprotein powder and water
Mixing carries out broken homogenization using high pressure homogenizer later, and homogeneous is primary under the conditions of 170MPa, after obtaining broken homogeneous
Amino acid mycoprotein.
2) it digests: taking amino acid mycoprotein after the broken homogeneous in step 1), adjusted pH using sodium hydroxide solution
It is 7.5,3% neutral proteinase of amino acid mycoprotein quality after broken homogeneous is added later, digests 12h, hydrolysis temperature
It is 60 DEG C.Neutral proteinase includes the raw neutral proteinase and papain of producing bacillus subtilis, withered in neutral proteinase
The mass ratio between neutral proteinase and papain that careless bacillus generates is 7:3.Enzyme deactivation is carried out after enzymatic hydrolysis, is gone out
The temperature of enzyme is 90 DEG C, and the enzyme deactivation time is 50min.Material after must being digested after enzyme deactivation.
3) dry: take material after the enzymatic hydrolysis in step 2) to be spray-dried, 170 DEG C of the intake air temperature of spray drying,
70 DEG C of air outlet temperature to get.
The enzymolysis protein feedstuff of the present embodiment, i.e., using the present embodiment enzymatic hydrolysis Amino acid fermentation bacteria method prepare and
?.
Embodiment 4
The method of the enzymatic hydrolysis Amino acid fermentation bacteria of the present embodiment, comprising the following steps:
1) pretreatment of raw material: taking amino acid mycoprotein, and amino acid mycoprotein includes glu thalline protein, tryptophan
Mycoprotein, lysine bacteria body protein, serine mycoprotein.Amino acid mycoprotein is subjected to powder using beater disintegrating machine
It is broken, 80 meshes are crossed after crushing up to amino acid mycoprotein powder.According to mass ratio it is 1 by amino acid mycoprotein powder and water:
2.5 mixing carry out broken homogenization using high pressure homogenizer later, and homogeneous is primary under the conditions of 170MPa, obtain broken equal
Amino acid mycoprotein after matter.
2) it digests: taking amino acid mycoprotein after the broken homogeneous in step 1), adjusted pH using sodium hydroxide solution
It is 7.0,4% neutral proteinase of amino acid mycoprotein quality after broken homogeneous is added later, digests for 24 hours, hydrolysis temperature
It is 50 DEG C.Neutral proteinase includes the raw neutral proteinase and papain of producing bacillus subtilis, withered in neutral proteinase
The mass ratio between neutral proteinase and papain that careless bacillus generates is 8:2.Enzyme deactivation is carried out after enzymatic hydrolysis, is gone out
The temperature of enzyme is 80 DEG C, and the enzyme deactivation time is 40min.Material after must being digested after enzyme deactivation.
3) dry: take material after the enzymatic hydrolysis in step 2) to be spray-dried, 180 DEG C of the intake air temperature of spray drying,
85 DEG C of air outlet temperature to get.
The enzymolysis protein feedstuff of the present embodiment, i.e., using the present embodiment enzymatic hydrolysis Amino acid fermentation bacteria method prepare and
?.
Embodiment 5
The method of the enzymatic hydrolysis Amino acid fermentation bacteria of the present embodiment, comprising the following steps:
1) pretreatment of raw material: taking amino acid mycoprotein, and amino acid mycoprotein includes glu thalline protein, tryptophan
Mycoprotein, lysine bacteria body protein, serine mycoprotein.Amino acid mycoprotein is subjected to powder using beater disintegrating machine
It is broken, 80 meshes are crossed after crushing up to amino acid mycoprotein powder.According to mass ratio it is 1 by amino acid mycoprotein powder and water:
3.5 mixing carry out broken homogenization using high pressure homogenizer later, and homogeneous is primary under the conditions of 170MPa, obtain broken equal
Amino acid mycoprotein after matter.
2) it digests: taking amino acid mycoprotein after the broken homogeneous in step 1), adjusted pH using sodium hydroxide solution
It is 8.0,2% neutral proteinase of amino acid mycoprotein quality after broken homogeneous is added later, digests for 24 hours, hydrolysis temperature
It is 60 DEG C.Neutral proteinase includes the raw neutral proteinase and papain of producing bacillus subtilis, withered in neutral proteinase
The mass ratio between neutral proteinase and papain that careless bacillus generates is 8:3.Enzyme deactivation is carried out after enzymatic hydrolysis, is gone out
The temperature of enzyme is 70 DEG C, and the enzyme deactivation time is 30min.Material after must being digested after enzyme deactivation.
3) dry: take material after the enzymatic hydrolysis in step 2) to be spray-dried, 170 DEG C of the intake air temperature of spray drying,
60 DEG C of air outlet temperature to get.
The enzymolysis protein feedstuff of the present embodiment, i.e., using the present embodiment enzymatic hydrolysis Amino acid fermentation bacteria method prepare and
?.
Test example
The enzymolysis feed albumen of Example 1 tests its crude protein content, small peptide content, protein solubility and stomach
Protease digestion rate, test method and test result are as shown in table 1.
The test method and test result of the indices of the amino acid mycoprotein enzymatic hydrolysis front and back of 1 embodiment 1 of table
| Index name | Before enzymatic hydrolysis | After enzymatic hydrolysis | Detection method |
| Crude protein | 71.8% | 72.5% | GB/T 6432-2018 |
| Small peptide | 4.7% | 54.3% | GB/T 22492-2008 |
| Protein solubility | 14.8% | 75.5% | DB13/T 812-2006 |
| Pepsin digestibility | 57.9% | 95.3% | GB/T 17811-2008 |
Seen from table 1, the product after enzymatic hydrolysis substantially improves palatability, and small peptide content is up to 50%, pepsin
Digestibility is greater than 90%, and protein solubility is greater than 70%, greatly improves the utilization efficiency of albumen.
Claims (10)
1. the method for digesting Amino acid fermentation bacteria, which comprises the following steps:
1) pretreatment of raw material: taking amino acid mycoprotein and crushing obtains amino acid mycoprotein powder, by amino acid mycoprotein
Powder is that 1:2.5~1:4 is mixed according to mass ratio with water, carries out broken homogenization later, obtains amino acid bacterium after broken homogeneous
Body protein；
2) it digests: taking amino acid mycoprotein after the broken homogeneous in step 1), adjusting pH is neutrality, and broken homogeneous is added later
1~4% neutral proteinase of amino acid mycoprotein quality carries out enzymatic hydrolysis 12~for 24 hours afterwards, and enzyme deactivation is carried out after enzymatic hydrolysis, is obtained
Material after enzymatic hydrolysis；
3) dry: take after the enzymatic hydrolysis in step 2) material to be spray-dried to get.
2. the method for enzymatic hydrolysis Amino acid fermentation bacteria according to claim 1, which is characterized in that digested described in step 2)
PH be 7.0~8.0, hydrolysis temperature be 40~60 DEG C.
3. the method for enzymatic hydrolysis Amino acid fermentation bacteria according to claim 2, which is characterized in that enzyme deactivation described in step 2
Temperature be 70~90 DEG C, the enzyme deactivation time be 20~50min.
4. the method for enzymatic hydrolysis Amino acid fermentation bacteria according to claim 1, which is characterized in that neutrality described in step 2)
Protease includes one or both of the raw neutral proteinase of producing bacillus subtilis or papain.
5. the method for enzymatic hydrolysis Amino acid fermentation bacteria according to claim 4, which is characterized in that in the neutral proteinase
Mass ratio between producing bacillus subtilis raw neutral proteinase and papain is 9:1~7:3.
6. the method for enzymatic hydrolysis Amino acid fermentation bacteria according to claim 1, which is characterized in that amino described in step 1)
Sour mycoprotein is glu thalline protein, tryptophan mycoprotein, lysine bacteria body protein, one in serine mycoprotein
Kind is a variety of.
7. the method for enzymatic hydrolysis Amino acid fermentation bacteria according to claim 1, which is characterized in that be crushed described in step 1)
Homogeneous is to be handled using high pressure homogenizer, and homogeneous is that homogeneous is primary under the conditions of 170MPa.
8. the method for enzymatic hydrolysis Amino acid fermentation bacteria according to claim 1, which is characterized in that spraying described in step 3)
160~180 DEG C of dry intake air temperature, 65~85 DEG C of air outlet temperature.
9. the method for enzymatic hydrolysis Amino acid fermentation bacteria according to claim 1, which is characterized in that crushed described in step 1)
Cross 80 meshes later up to the amino acid mycoprotein powder.
10. the enzymolysis protein feedstuff of the method preparation using enzymatic hydrolysis Amino acid fermentation bacteria as described in claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910553243.0A CN110305928A (en) | 2019-06-25 | 2019-06-25 | Digest the method and enzymolysis protein feedstuff of Amino acid fermentation bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910553243.0A CN110305928A (en) | 2019-06-25 | 2019-06-25 | Digest the method and enzymolysis protein feedstuff of Amino acid fermentation bacteria |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110305928A true CN110305928A (en) | 2019-10-08 |
Family
ID=68076750
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910553243.0A Pending CN110305928A (en) | 2019-06-25 | 2019-06-25 | Digest the method and enzymolysis protein feedstuff of Amino acid fermentation bacteria |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110305928A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110663813A (en) * | 2019-11-07 | 2020-01-10 | 中国海洋大学 | Fish meal microbial fermentation and enzymolysis method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102696856A (en) * | 2012-04-18 | 2012-10-03 | 马长庆 | Microbial protein hydrolysate and preparation method and application thereof |
| CN107494891A (en) * | 2017-07-20 | 2017-12-22 | 广东肇庆星湖生物科技股份有限公司 | A kind of method that mycoprotein makes animal food additive |
| CN107549760A (en) * | 2017-09-19 | 2018-01-09 | 广东肇庆星湖生物科技股份有限公司 | A kind of preparation method of compound nutritional flavoring agent |
| CN108913605A (en) * | 2018-07-05 | 2018-11-30 | 南通博亿化工有限公司 | A kind of thallus recycling technique |
-
2019
- 2019-06-25 CN CN201910553243.0A patent/CN110305928A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102696856A (en) * | 2012-04-18 | 2012-10-03 | 马长庆 | Microbial protein hydrolysate and preparation method and application thereof |
| CN107494891A (en) * | 2017-07-20 | 2017-12-22 | 广东肇庆星湖生物科技股份有限公司 | A kind of method that mycoprotein makes animal food additive |
| CN107549760A (en) * | 2017-09-19 | 2018-01-09 | 广东肇庆星湖生物科技股份有限公司 | A kind of preparation method of compound nutritional flavoring agent |
| CN108913605A (en) * | 2018-07-05 | 2018-11-30 | 南通博亿化工有限公司 | A kind of thallus recycling technique |
Non-Patent Citations (1)
| Title |
|---|
| 姚菁华等: "谷氨酸菌体蛋白的酶解试验研究", 《淮海工学院学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110663813A (en) * | 2019-11-07 | 2020-01-10 | 中国海洋大学 | Fish meal microbial fermentation and enzymolysis method |
| CN110663813B (en) * | 2019-11-07 | 2022-04-08 | 中国海洋大学 | Fish meal microbial fermentation and enzymolysis method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102048022B (en) | Soybean polypeptide without hydrolysis and bitter tastes as well as preparation method and application thereof | |
| CN103053794B (en) | Method for preparing prebiotics fermented feed by using bagasse and molasses | |
| CN101584375B (en) | Method for producing chicken manure fermented feed | |
| CN104171759A (en) | Edible mushroom residue feed preparation process | |
| CN104762234A (en) | Complex bacterium for bean pulp fermentation and application of complex bacterium | |
| CN102178037B (en) | Method for producing feed of high-yield dairy cow by using corn straws fermented by Rhodotorula glutinis | |
| CN101209082A (en) | Preparation of functional feed protein dried porcine saluble | |
| CN103667221B (en) | Alkaline xylanase-containing dedicated enzyme for piglets and preparation method of alkaline xylanase-containing dedicated enzyme | |
| CN101019575A (en) | Production process of composite animal and plant protein sour milk | |
| CN106578402A (en) | High-protein fermented soybean meal and preparation method thereof | |
| CN102823735A (en) | Application of aqueous extract of grass-cultivated ganoderma bacterium draff in dairy cow feeding | |
| CN104839457A (en) | Method for preparing small peptide-enriched protein feed material based on steam explosion in combination with solid enzymatic hydrolysis | |
| CN101126103A (en) | Method for preparing corn protein peptide with high F valve by microorganism zymotechnics | |
| CN104212863A (en) | Preparation method of feed-use animal protein peptide | |
| CN105018558B (en) | A kind of clover multiple target coproduction extraction separation method | |
| CN103931776A (en) | Production process of bean dregs powder | |
| CN102660622A (en) | Method for preparing bioactive soybean peptide by mixed culture solid-state fermentation | |
| CN111642730A (en) | Soy sauce residue cyclic elution utilization method, product and equipment | |
| CN104171265A (en) | Processing technology of single-cell protein from distilled spirit lees | |
| CN111328934A (en) | Enzymolysis method of chicken intestinal tract, carp feed containing chicken intestinal tract and preparation method of feed | |
| CN110305928A (en) | Digest the method and enzymolysis protein feedstuff of Amino acid fermentation bacteria | |
| CN104745650A (en) | Preparation method of combined-state glutamine used for improving nutrients of piglet intestines | |
| CN102150806A (en) | Method for producing novel ossein flavor powder through ultrasonic auxiliary spray drying | |
| CN104585513A (en) | Production method for feather oligopeptide chelating type mineral element | |
| CN101444289A (en) | Technology for simultaneously preparing cooking wine and slimming food |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191008 |