CN110250053A - A kind of optomotor response test macro for zebra fish research - Google Patents
A kind of optomotor response test macro for zebra fish research Download PDFInfo
- Publication number
- CN110250053A CN110250053A CN201910677453.0A CN201910677453A CN110250053A CN 110250053 A CN110250053 A CN 110250053A CN 201910677453 A CN201910677453 A CN 201910677453A CN 110250053 A CN110250053 A CN 110250053A
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- zebra fish
- optomotor response
- optomotor
- test macro
- test
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- 238000012360 testing method Methods 0.000 title claims abstract description 75
- 241000252212 Danio rerio Species 0.000 title claims abstract description 46
- 230000026269 optomotor response Effects 0.000 title claims abstract description 37
- 238000011160 research Methods 0.000 title claims abstract description 16
- 241000251468 Actinopterygii Species 0.000 claims abstract description 19
- 230000004044 response Effects 0.000 claims abstract description 12
- 230000002776 aggregation Effects 0.000 claims abstract description 6
- 238000004220 aggregation Methods 0.000 claims abstract description 6
- 239000000523 sample Substances 0.000 claims description 16
- 208000003443 Unconsciousness Diseases 0.000 claims description 7
- 239000004744 fabric Substances 0.000 claims description 7
- 238000005286 illumination Methods 0.000 claims description 3
- 230000000875 corresponding effect Effects 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
- 230000033001 locomotion Effects 0.000 description 5
- 238000012544 monitoring process Methods 0.000 description 5
- 230000007547 defect Effects 0.000 description 4
- 206010064571 Gene mutation Diseases 0.000 description 3
- 230000029264 phototaxis Effects 0.000 description 3
- 238000003384 imaging method Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 108700024526 zebrafish sox32 Proteins 0.000 description 2
- 241000283070 Equus zebra Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000004424 eye movement Effects 0.000 description 1
- 230000000366 juvenile effect Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 108010008359 protein kinase C lambda Proteins 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/003—Aquaria; Terraria
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/06—Arrangements for heating or lighting in, or attached to, receptacles for live fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Zoology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention discloses a kind of optomotor response test macros for zebra fish research, comprising: a plurality of testing tubes being hinged, and it is connected to the acquisition component of test tube end；The hinged place of wantonly two testing tube is provided with test cavity, and the external side of test chamber is arranged at intervals with several gratings, and by the diverging or aggregation of grating, the shoal of fish can make corresponding population response；Several described gratings can circumferentially move in a circle along the test cavity, by changing the parameter of grating, test the optomotor response of fish；The acquisition component can carry out image collection to the optomotor response of fish.
Description
Technical field
The present invention relates to a kind of optomotor response test macro more particularly to a kind of optomotor response surveys for zebra fish research
Test system.
Background technique
Zebra fish as a kind of new ant algorithms animal, have the characteristics that it is many unusual, zebra fish have eye movement reaction,
Optomotor response etc., optomotor response refer to that zebra fish has certain chasing behavior to mobile target, which can be used to detect
The visual correlation gene mutation of juvenile fish and adult fish can screen and change unresponsive individual to grating orientation, to reach screening
The purpose of vision gene mutation defect body.
Traditional optomotor response test macro is merely capable of test zebra fish to the optomotor response of light source, can not detect and
The individual of zebra fish vision gene mutation is screened, and the view of detailed test zebra fish cannot be carried out according to the parameters of grating
Dynamic stress reaction, can not carry out comprehensive test.
Summary of the invention
The present invention overcomes the deficiencies in the prior art, provide a kind of optomotor response test macro for zebra fish research.
In order to achieve the above objectives, a kind of the technical solution adopted by the present invention are as follows: optomotor response survey for zebra fish research
Test system, comprising: a plurality of testing tubes being hinged, and it is connected to the acquisition component of test tube end；Wantonly two testing tube
Hinged place be provided with test cavity, the external side of test chamber is arranged at intervals with several gratings, by the diverging of grating or
Aggregation, the shoal of fish can make corresponding population response；Several described gratings can circumferentially move in a circle along the test cavity,
By changing the parameter of grating, the optomotor response of fish is tested；The acquisition component can be to the optomotor response of fish
Carry out image collection.
In a preferred embodiment of the present invention, the acquisition component includes several probes, several described probes are along survey
Test tube length direction is spaced apart, the probe, is capable of the optomotor response of real-time monitoring zebra fish.
In a preferred embodiment of the present invention, the probe can be adjusted the angle.
In a preferred embodiment of the present invention, the parameter of the grating includes the bright of rotation direction, rotational frequency or grating
Degree.
It further include several illuminators in a preferred embodiment of the present invention, several described illuminators are along the test
Length of tube direction is spaced apart.
In a preferred embodiment of the present invention, the illuminator can adjust different intensity of illumination or penetrability.
In a preferred embodiment of the present invention, black-out cloth is provided on the outside of the testing tube.
In a preferred embodiment of the present invention, the collected zebra fish image information of acquisition component is transmitted to calculating
Machine.
In a preferred embodiment of the present invention, the size of the test cavity is greater than the size of the testing tube.
The invention solves the defect existing in the background technology, the present invention have it is following the utility model has the advantages that
(1) pass through the movement or circumferential movement of grating, zebra fish can move towards the mobile direction of grating, a plurality of zebra fish
When being tested, grating dispersion or aggregation, the shoal of fish can show corresponding population response, pass through the population response of the shoal of fish, analysis
The optomotor response of zebra fish.
(2) the corresponding effect shown by the optomotor response of zebra fish, is able to detect the vision response of zebra fish, from
And the zebra fish that relative response is slow in group can be filtered out, and then the individual of the zebra fish defects of vision can be picked out, it mentions
The optimized varieties of high zebra fish.
(3) by changing the rotation time frequency or spatial frequency of grating, the difference for carrying out test zebra fish follows preference,
And stress reaction, real-time imaging monitoring is then carried out by probe, improves the flexibility of apparent motion test macro, and monitoring essence
Degree.
(4) black-out cloth on the outside of testing tube can block ambient light photograph, can by adjusting the lightproof area of black-out cloth
Zebra fish is tested to the phototaxis and terrified stress reaction of light source.
Detailed description of the invention
Fig. 1 is the partial structural diagram of the preferred embodiment of the present invention；
Fig. 2 is the acquisition component partial structural diagram of the preferred embodiment of the present invention；
Fig. 3 is the acquisition component partial enlargement diagram of the preferred embodiment of the present invention；
Fig. 4 is the test cavity partial structural diagram of the preferred embodiment of the present invention；
Fig. 5 is the test inside cavity structural schematic diagram of the preferred embodiment of the present invention；
Appended drawing reference:
1, testing tube, 2, illuminator, 3, test cavity, 4, probe, 5, acquisition component, 6, grating, 7, camera.
Specific embodiment
Presently in connection with drawings and examples, the present invention is described in further detail, these attached drawings are simplified signal
Figure, the basic structure of the invention will be illustrated schematically only, therefore it only shows the composition relevant to the invention.
As shown in Figs. 1-5, a kind of optomotor response test macro for zebra fish research, comprising: 2 surveys being hinged
Test tube 1, and it is connected to the composition of acquisition component 5 of 1 end of testing tube；
Specifically, the hinged place of every two testing tube 1 is provided with test cavity 3, test 3 outside spacers of cavity are provided with
8 gratings 6 realize the diverging or aggregation of grating 6, it is anti-that the shoal of fish can make corresponding group by the mutual cooperation of 8 gratings 6
It answers；8 gratings 6 can circumferentially move in a circle along test cavity 3, by changing the parameter of grating 6, to the optomotor response of fish
It is tested；Acquisition component 5 can carry out image collection to the optomotor response of fish, and test on 3 side inner wall of cavity and also set
3 cameras 7 are equipped with, it being capable of zebra in accurate collecting test cavity 3 or testing tube 1 by probe 4 and the cooperation of camera 7
The optomotor response of fish improves the accuracy of experiment.
Acquisition component 5 includes that 2 probes, 4,2 probes 4 are spaced apart along 1 length direction of testing tube, probe 4, Neng Goushi
When monitor the optomotor response of zebra fish, probe 4 can adjust the angle, and pass through the rotation time frequency for changing grating 6 or space frequency
Rate, the difference for carrying out test zebra fish follow preference and stress reaction, then carry out real-time imaging monitoring by probe 4, mention
The flexibility of high apparent motion test macro and monitoring accuracy.
The parameter of grating 6 includes the brightness of rotation direction, rotational frequency or grating 6, passes through the movement or circumferential fortune of grating 6
Dynamic, zebra fish can move towards the mobile direction of grating 6, when a plurality of zebra fish is tested, the diverging of grating 6 or aggregation, and the shoal of fish
Corresponding population response can be shown, by the population response of the shoal of fish, analyzes the optomotor response of zebra fish.
It preferably, in a specific embodiment of the invention further include 2-8 illuminator 2, illuminator 2 is long along testing tube 1
Degree direction is spaced apart, and illuminator 2 can adjust different intensity of illumination or penetrability, so that the phototaxis of zebra fish is tested,
And the sensitive difference of vision response.
Black-out cloth is provided on the outside of testing tube 1, the black-out cloth in 1 outside of testing tube can block ambient light photograph, by adjusting
The lightproof area of black-out cloth can test zebra fish to the phototaxis and terrified stress reaction of light source, and acquisition component 5 acquires
To zebra fish image information be transmitted to computer, the size of test cavity 3 is greater than the size of testing tube 1,
The corresponding effect shown by the optomotor response of zebra fish, is able to detect the vision response of zebra fish, thus
The zebra fish that relative response is slow in group can be filtered out, and then the individual of the zebra fish defects of vision can be picked out, is improved
The optimized varieties of zebra fish.
Based on the above description of the preferred embodiments of the present invention, through the above description, related personnel completely can be with
Without departing from the scope of the technological thought of the present invention', various changes and amendments are carried out.The technical scope of this invention
It is not limited to the contents of the specification, it is necessary to determine the technical scope according to the scope of the claims.
Claims (9)
1. a kind of optomotor response test macro for zebra fish research characterized by comprising a plurality of surveys being hinged
Test tube, and it is connected to the acquisition component of test tube end；
The hinged place of wantonly two testing tube is provided with test cavity, and the external side of test chamber is arranged at intervals with several gratings, leads to
The diverging or aggregation of grating are crossed, the shoal of fish can make corresponding population response；
Several described gratings can circumferentially move in a circle along the test cavity, by changing the parameter of grating, to fish
Optomotor response tested；
The acquisition component can carry out image collection to the optomotor response of fish.
2. a kind of optomotor response test macro for zebra fish research according to claim 1, it is characterised in that: described
Acquisition component includes several probes, several described probes are spaced apart along testing tube length direction, the probe, Neng Goushi
When monitor zebra fish optomotor response.
3. a kind of optomotor response test macro for zebra fish research according to claim 2, it is characterised in that: described
Probe can adjust the angle.
4. a kind of optomotor response test macro for zebra fish research according to claim 1, it is characterised in that: described
The parameter of grating includes the brightness of rotation direction, rotational frequency or grating.
5. a kind of optomotor response test macro for zebra fish research according to claim 1, it is characterised in that: also wrap
Several illuminators are included, several described illuminators are spaced apart along the testing tube length direction.
6. a kind of optomotor response test macro for zebra fish research according to claim 5, it is characterised in that: described
Illuminator can adjust different intensity of illumination or penetrability.
7. a kind of optomotor response test macro for zebra fish research according to claim 1, it is characterised in that: described
Black-out cloth is provided on the outside of testing tube.
8. a kind of optomotor response test macro for zebra fish research according to claim 1, it is characterised in that: described
The collected zebra fish image information of acquisition component is transmitted to computer.
9. a kind of optomotor response test macro for zebra fish research according to claim 1, it is characterised in that: described
The size for testing cavity is greater than the size of the testing tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910677453.0A CN110250053B (en) | 2019-07-25 | 2019-07-25 | Vision movement reaction testing system for zebra fish research |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910677453.0A CN110250053B (en) | 2019-07-25 | 2019-07-25 | Vision movement reaction testing system for zebra fish research |
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| Publication Number | Publication Date |
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| CN110250053A true CN110250053A (en) | 2019-09-20 |
| CN110250053B CN110250053B (en) | 2021-09-14 |
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ID=67928209
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910677453.0A Active CN110250053B (en) | 2019-07-25 | 2019-07-25 | Vision movement reaction testing system for zebra fish research |
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| CN (1) | CN110250053B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111134062A (en) * | 2020-01-07 | 2020-05-12 | 中国水产科学研究院南海水产研究所 | A device for testing stress-resistant reaction of penaeus monodon |
| CN111165405A (en) * | 2020-01-03 | 2020-05-19 | 中国水产科学研究院南海水产研究所 | Penaeus monodon behavior preference test system and test method |
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|---|---|---|---|---|
| CN102172225A (en) * | 2011-02-28 | 2011-09-07 | 中国科学技术大学 | Zebra fish behavior induction and analytical method and system |
| CN102613127A (en) * | 2012-04-06 | 2012-08-01 | 中国水产科学研究院淡水渔业研究中心 | Device suitable for studying phototactic ethology of minitype fishes |
| CN103202254A (en) * | 2013-04-16 | 2013-07-17 | 中国水产科学研究院长江水产研究所 | Fish swimming capability measuring method and submerged open-type testing device |
| CN104969898A (en) * | 2015-07-24 | 2015-10-14 | 中国水产科学研究院黄海水产研究所 | Experiment device used for researching effects on fish behaviors imposed by light and application thereof |
| CN205848361U (en) * | 2016-08-01 | 2017-01-04 | 三峡大学 | A kind of experimental provision for Fish phototaxis research |
| CN210202920U (en) * | 2019-07-25 | 2020-03-31 | 广东中科英海科技有限公司 | Vision movement reaction testing system for zebra fish research |
| JP2020110079A (en) * | 2019-01-11 | 2020-07-27 | 株式会社ＦｕｌｌＤｅｐｔｈ | Fish monitoring system |
-
2019
- 2019-07-25 CN CN201910677453.0A patent/CN110250053B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102172225A (en) * | 2011-02-28 | 2011-09-07 | 中国科学技术大学 | Zebra fish behavior induction and analytical method and system |
| CN102613127A (en) * | 2012-04-06 | 2012-08-01 | 中国水产科学研究院淡水渔业研究中心 | Device suitable for studying phototactic ethology of minitype fishes |
| CN103202254A (en) * | 2013-04-16 | 2013-07-17 | 中国水产科学研究院长江水产研究所 | Fish swimming capability measuring method and submerged open-type testing device |
| CN104969898A (en) * | 2015-07-24 | 2015-10-14 | 中国水产科学研究院黄海水产研究所 | Experiment device used for researching effects on fish behaviors imposed by light and application thereof |
| CN205848361U (en) * | 2016-08-01 | 2017-01-04 | 三峡大学 | A kind of experimental provision for Fish phototaxis research |
| JP2020110079A (en) * | 2019-01-11 | 2020-07-27 | 株式会社ＦｕｌｌＤｅｐｔｈ | Fish monitoring system |
| CN210202920U (en) * | 2019-07-25 | 2020-03-31 | 广东中科英海科技有限公司 | Vision movement reaction testing system for zebra fish research |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111165405A (en) * | 2020-01-03 | 2020-05-19 | 中国水产科学研究院南海水产研究所 | Penaeus monodon behavior preference test system and test method |
| CN111134062A (en) * | 2020-01-07 | 2020-05-12 | 中国水产科学研究院南海水产研究所 | A device for testing stress-resistant reaction of penaeus monodon |
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| Publication number | Publication date |
|---|---|
| CN110250053B (en) | 2021-09-14 |
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