CN210202920U

CN210202920U - Vision movement reaction testing system for zebra fish research

Info

Publication number: CN210202920U
Application number: CN201921181616.8U
Authority: CN
Inventors: Xiangying Li; 李祥英; Zhihong Li; 李志鸿; Yunqi Zhou; 周韵琦; Yan Wu; 吴妍; Rui Zhang; 张蕊; Qiyuan Wu; 吴启源
Original assignee: Guangdong Zhongke Yinghai Technology Co Ltd
Current assignee: Guangdong Zhongke Yinghai Technology Co Ltd
Priority date: 2019-07-25
Filing date: 2019-07-25
Publication date: 2020-03-31
Anticipated expiration: 2029-07-25

Abstract

The utility model discloses a look moves response test system for zebra fish research, include: the device comprises a plurality of mutually hinged test tubes and a collecting assembly connected to the end parts of the test tubes; a testing cavity is arranged at the hinged position of any two testing pipes, a plurality of gratings are arranged at intervals outside the testing cavity, and fish schools can make corresponding group reactions through divergence or convergence of the gratings; the plurality of gratings can do circular motion along the circumferential direction of the test cavity, and the optokinetic reaction of the fish is tested by changing the parameters of the gratings; the collection assembly can collect images of the visual-kinetic reaction of the fish.

Description

Vision movement reaction testing system for zebra fish research

Technical Field

The utility model relates to a look and move reaction test system, especially relate to a look and move reaction test system for zebra fish research.

Background

The zebra fish as a novel model animal has many distinctive characteristics, has eye movement reaction, visual movement reaction and the like, wherein the visual movement reaction refers to certain pursuit behavior of the zebra fish to a moving target, can be used for detecting visual related gene mutation of juvenile fish and adult fish, and can be used for screening individuals with slow response to raster direction change so as to achieve the purpose of screening visual gene mutation defect bodies.

The traditional optokinetic reaction test system can only test the optokinetic reaction of the zebra fish to a light source, cannot detect and screen individuals with the zebra fish visual gene mutation, cannot test the optokinetic stress reaction of the zebra fish in detail according to each parameter of the grating, and cannot test the zebra fish in an all-round way.

SUMMERY OF THE UTILITY MODEL

The utility model overcomes prior art's is not enough, provides a look moves reaction test system for zebra fish research.

In order to achieve the above purpose, the utility model adopts the technical scheme that: a optokinetic response testing system for zebrafish research comprising: the device comprises a plurality of mutually hinged test tubes and a collecting assembly connected to the end parts of the test tubes; a testing cavity is arranged at the hinged position of any two testing pipes, a plurality of gratings are arranged at intervals outside the testing cavity, and fish schools can make corresponding group reactions through divergence or convergence of the gratings; the plurality of gratings can do circular motion along the circumferential direction of the test cavity, and the optokinetic reaction of the fish is tested by changing the parameters of the gratings; the collection assembly can collect images of the visual-kinetic reaction of the fish.

The utility model discloses a preferred embodiment, the collection subassembly includes a plurality of probe, a plurality of the probe is along test tube length direction interval distribution, the probe can real-time supervision zebra fish's apparent reaction of moving.

In a preferred embodiment of the present invention, the probe can adjust the angle.

In a preferred embodiment of the present invention, the parameter of the grating includes a rotation direction, a rotation frequency or a brightness of the grating.

The utility model discloses a preferred embodiment still includes a plurality of light source lamp, a plurality of light source lamp follows test tube length direction interval distribution.

In a preferred embodiment of the present invention, the light source lamp can adjust different illumination intensities or penetrability.

In a preferred embodiment of the present invention, the outer side of the testing tube is provided with a shading cloth.

In a preferred embodiment of the present invention, the zebra fish image information collected by the collecting component is transmitted to a computer.

In a preferred embodiment of the present invention, the size of the testing chamber is larger than the size of the testing tube.

The utility model provides a defect that exists among the background art, the utility model discloses possess following beneficial effect:

(1) through the movement or circumferential movement of the grating, the zebra fish can move towards the direction of the movement of the grating, when a plurality of zebra fish are tested, the grating diverges or gathers, the fish school can show corresponding group reaction, and the optokinetic reaction of the zebra fish is analyzed through the group reaction of the fish school.

(2) The visual response of the zebra fish can be detected through the corresponding effect shown by the visual response of the zebra fish, so that the zebra fish with relatively slow response in a group can be screened out, individuals with visual defects of the zebra fish can be selected out, and the variety optimization of the zebra fish is improved.

(3) Through changing the rotation time frequency or the space frequency of the grating, different following preferences and stress reactions of the zebra fish are tested, and then real-time image monitoring is carried out through the probe, so that the flexibility and the monitoring precision of the visual motion testing system are improved.

(4) The shading cloth outside the test tube can shade outside illumination, and the phototaxis of the zebra fish to the light source and the frightened stress response can be tested by adjusting the shading area of the shading cloth.

Drawings

Fig. 1 is a partial schematic structure diagram of a preferred embodiment of the present invention;

fig. 2 is a schematic view of a partial structure of the collecting assembly according to the preferred embodiment of the present invention;

fig. 3 is a partially enlarged schematic view of the acquisition assembly of the preferred embodiment of the present invention;

fig. 4 is a schematic view of a partial structure of a test chamber according to a preferred embodiment of the present invention;

fig. 5 is a schematic view of the internal structure of the testing chamber according to the preferred embodiment of the present invention;

reference numerals:

1. the device comprises a test tube, 2, a light source lamp, 3, a test cavity, 4, a probe, 5, an acquisition assembly, 6, a grating, 7 and a camera.

Detailed Description

The invention will now be described in further detail with reference to the accompanying drawings, which are simplified schematic drawings and illustrate, by way of illustration only, the basic structure of the invention, and which therefore show only the constituents relevant to the invention.

As shown in fig. 1 to 5, a visual motor reaction test system for zebrafish research comprises: 2 mutually hinged test tubes 1 and a collection assembly 5 connected to the end part of the test tube 1;

specifically, a testing cavity 3 is arranged at the hinged position of every two testing tubes 1, 8 gratings 6 are arranged at the outer side of the testing cavity 3 at intervals, the divergence or gathering of the gratings 6 is realized through the mutual matching of the 8 gratings 6, and fish schools can make corresponding group reactions; the 8 gratings 6 can do circular motion along the circumferential direction of the testing cavity 3, and the optokinetic reaction of the fish is tested by changing the parameters of the gratings 6; gather subassembly 5 and can carry out image acquisition to the look of fish and move the reaction, and also be provided with 3 cameras 7 on the inner wall of 3 one sides of test cavity, the look of zebra fish moves the reaction in collection test cavity 3 or test tube 1 that can be accurate through probe 4 and camera 7's cooperation, improves the accurate nature of experiment.

Gather subassembly 5 and include 2

probes

4, 2 probes 4 are along 1 length direction interval distribution of test tube, probe 4, can real-time supervision zebra fish look the reaction of moving, probe 4 can adjust the angle, through the rotation time frequency or the spatial frequency who change grating 6, test zebra fish's difference and follow the preference, and stress response, then carry out real-time image monitoring through probe 4, improve and look the flexibility of moving test system, and the monitoring precision.

The parameters of the grating 6 comprise a rotating direction, a rotating frequency or the brightness of the grating 6, the zebra fish can move towards the moving direction of the grating 6 through the movement or circumferential movement of the grating 6, when a plurality of zebra fish are tested, the grating 6 is dispersed or gathered, the fish school can show corresponding group reaction, and the visual kinetic reaction of the zebra fish is analyzed through the group reaction of the fish school.

Preferably, the utility model discloses a still include 2-8 light source lamp 2 among the embodiment, light source lamp 2 is along 1 length direction interval distribution of test tube, and light source lamp 2 can adjust different illumination intensity or penetrability to the phototaxis of test zebra fish, and the sensitive difference of visual response.

The shading cloth is arranged on the outer side of the test tube 1, the shading cloth on the outer side of the test tube 1 can shade outside illumination, the phototaxis and the frightened stress response of the zebra fish to a light source can be tested by adjusting the shading area of the shading cloth, the zebra fish image information collected by the collecting assembly 5 is transmitted to a computer, the size of the test cavity 3 is larger than that of the test tube 1,

the visual response of the zebra fish can be detected through the corresponding effect shown by the visual response of the zebra fish, so that the zebra fish with relatively slow response in a group can be screened out, individuals with visual defects of the zebra fish can be selected out, and the variety optimization of the zebra fish is improved.

In light of the foregoing, it is to be understood that various changes and modifications may be made by those skilled in the art without departing from the spirit and scope of the invention. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.

Claims

1. A optokinetic response testing system for zebrafish research, comprising: the device comprises a plurality of mutually hinged test tubes and a collecting assembly connected to the end parts of the test tubes;

a testing cavity is arranged at the hinged position of any two testing pipes, a plurality of gratings are arranged at intervals outside the testing cavity, and fish schools can make corresponding group reactions through divergence or convergence of the gratings;

the plurality of gratings can do circular motion along the circumferential direction of the test cavity, and the optokinetic reaction of the fish is tested by changing the parameters of the gratings;

the collection assembly can collect images of the visual-kinetic reaction of the fish.

2. A visual acuity response testing system for zebrafish research according to claim 1, wherein: the collection assembly comprises a plurality of probes which are distributed at intervals along the length direction of the test tube, and the probes can monitor the optokinetic reaction of the zebra fish in real time.

3. A visual acuity response testing system for zebrafish research according to claim 2, wherein: the angle of the probe can be adjusted.

4. A visual acuity response testing system for zebrafish research according to claim 1, wherein: the parameters of the grating include a rotation direction, a rotation frequency, or a brightness of the grating.

5. A visual acuity response testing system for zebrafish research according to claim 1, wherein: the test tube also comprises a plurality of light source lamps which are distributed at intervals along the length direction of the test tube.

6. A optokinetic response testing system for zebrafish research according to claim 5, wherein: the light source lamp can adjust different illumination intensity or penetrability.

7. A visual acuity response testing system for zebrafish research according to claim 1, wherein: and shading cloth is arranged on the outer side of the test tube.

8. A visual acuity response testing system for zebrafish research according to claim 1, wherein: and the zebra fish image information acquired by the acquisition component is transmitted to a computer.

9. A visual acuity response testing system for zebrafish research according to claim 1, wherein: the size of the test cavity is larger than that of the test tube.