CN110248039A - A kind of rice leaf photographic method - Google Patents

A kind of rice leaf photographic method Download PDF

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Publication number
CN110248039A
CN110248039A CN201910519489.6A CN201910519489A CN110248039A CN 110248039 A CN110248039 A CN 110248039A CN 201910519489 A CN201910519489 A CN 201910519489A CN 110248039 A CN110248039 A CN 110248039A
Authority
CN
China
Prior art keywords
blade
rice leaf
waterproof paper
paper
rice
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201910519489.6A
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Chinese (zh)
Inventor
陈玲
陈越
柯学
程在全
钟巧芳
付坚
王波
张敦宇
余腾琼
王玲仙
肖素勤
殷富有
蒋聪
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biotechnology and Germplasm Resource Institute of Yunnan Academy of Agricultural Sciences
Original Assignee
Biotechnology and Germplasm Resource Institute of Yunnan Academy of Agricultural Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biotechnology and Germplasm Resource Institute of Yunnan Academy of Agricultural Sciences filed Critical Biotechnology and Germplasm Resource Institute of Yunnan Academy of Agricultural Sciences
Priority to CN201910519489.6A priority Critical patent/CN110248039A/en
Publication of CN110248039A publication Critical patent/CN110248039A/en
Pending legal-status Critical Current

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Classifications

    • GPHYSICS
    • G03PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03BAPPARATUS OR ARRANGEMENTS FOR TAKING PHOTOGRAPHS OR FOR PROJECTING OR VIEWING THEM; APPARATUS OR ARRANGEMENTS EMPLOYING ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ACCESSORIES THEREFOR
    • G03B15/00Special procedures for taking photographs; Apparatus therefor
    • HELECTRICITY
    • H04ELECTRIC COMMUNICATION TECHNIQUE
    • H04NPICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N1/00Scanning, transmission or reproduction of documents or the like, e.g. facsimile transmission; Details thereof
    • H04N1/00127Connection or combination of a still picture apparatus with another apparatus, e.g. for storage, processing or transmission of still picture signals or of information associated with a still picture
    • H04N1/00249Connection or combination of a still picture apparatus with another apparatus, e.g. for storage, processing or transmission of still picture signals or of information associated with a still picture with a photographic apparatus, e.g. a photographic printer or a projector
    • H04N1/00251Connection or combination of a still picture apparatus with another apparatus, e.g. for storage, processing or transmission of still picture signals or of information associated with a still picture with a photographic apparatus, e.g. a photographic printer or a projector with an apparatus for taking photographic images, e.g. a camera
    • HELECTRICITY
    • H04ELECTRIC COMMUNICATION TECHNIQUE
    • H04NPICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N1/00Scanning, transmission or reproduction of documents or the like, e.g. facsimile transmission; Details thereof
    • H04N1/04Scanning arrangements, i.e. arrangements for the displacement of active reading or reproducing elements relative to the original or reproducing medium, or vice versa

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  • Engineering & Computer Science (AREA)
  • Multimedia (AREA)
  • Signal Processing (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a kind of rice leaf photographic methods.This method include will be acquired from living body rice plant lower rice leaf be put into it is fresh-keeping in the test tube equipped with clear water, it takes out blade and prunes vacuum side of blade vein convex portion with blade, make vein and blade in approximately the same plane, equidistant vertical straight line is drawn on waterproof paper, vein on blade is aligned with vertical straight line, it is secured the vanes on waterproof paper with solid gum, blade is covered with wetting cotton paper towel during fixed blade, to prevent leaves water loss, after all blades fix, the waterproof paper for fixing blade is packed into valve bag, valve bag equipped with the waterproof paper for fixing blade is put in scanning on scanner and obtains photo.Whole process of the present invention all makes rice leaf be in fresh-keeping state, can prevent the quick dehydration of rice leaf and dry up eclipsed, it is ensured that the rice leaf sample photo of acquisition is fresh and alive, true to nature;It ensures leaf blade specimen marshalling, photo is obtained using scanner, simply, conveniently.

Description

A kind of rice leaf photographic method
Technical field
The invention belongs to biotechnology research fields, are related to a kind of rice leaf photographic method.
Background technique
Blade is that rice carries out photosynthesis, respiration and steaming as the most important component part of rice canopy structure The major organs of effect are risen, function has a major impact the yield and quality of rice.The various parameters pair of Study On Rice blade Growth and development, yield, quality and cultivation management of rice etc. are of great significance.For a long time, about Rice Leaf phase Close parameter study and be unequal to its number, such as Leaf pattern (including leaf, blade tip, leaf margin, phyllopodium and vein surface) build up research, Leaf color research and pest and disease damage relevant to leaf research.The successes achieved in research are usually with the outside announcement that has a learned dissertation published, rice The academic research of leaf relevant parameter is no exception, and when writing academic paper, technical journal is quite stringent to the image request of paper, Because image is the important form of technical paper statement, occupy considerable status in entire technical paper, image it is clear Chu, beauty are coordinated not only make paper discussion clear, clear, can also play active, beautification, save the space of a whole page, improve reader The effect of reading interest.When acquiring rice leaf picture, take pictures after excised leaf need to being fixed on paper, because rice is water Plant, for excised leaf once exposed in air, dehydrating speed is quite fast, and 1min or so blade just will appear curling, thus It here eclipsed withers.In addition, general sample wants smooth when taking pictures, the photo ability of acquisition is neat, coordinates, is generous, and rice leaf is in The vein protrusion of long lanceolar, generally long 20~400mm, wide 8~120mm, leaf back is significant, rice leaf is difficult to solid Determine in one plane, to influence effect of taking pictures.
Therefore, it is the image for obtaining beautiful, true to nature coordination, develops that a kind of rice leaf is convenient, fast, effectively takes pictures Method is very necessary.
Summary of the invention
Technical problem to be solved by the invention is to provide a kind of convenient, fast, effective rice leaf photographic methods.
A kind of rice leaf photographic method provided by the present invention, comprising the following steps: (1) preparation of consumptive material;(2) rice Leaf harvest and keeping;(3) position of fixed blade is made on waterproof paper;(4) blade is fixed on waterproof paper, is packed into simultaneously Valve bag saves;(5) valve bag equipped with the waterproof paper for fixing blade is put in scanning on scanner and obtains photo.
The preparation of consumptive material described in step (1) includes preparing solid gum, waterproof paper, ruler, pencil, blade, test tube, cotton paper Towel, toilet paper, valve bag, scanner and clear water.
The acquisition of rice leaf described in step (2) and keeping are as follows: the rice leaf that will be collected from living body rice plant Be put into the test tube equipped with clear water carry out it is fresh-keeping.
Making the position of fixed blade described in step (3) on waterproof paper is drawn on waterproof paper with ruler and pencil The a plurality of vertical straight line of distance at equal intervals, spacing distance size are collected in adjacent two vertical straight lines to be placed on vertically Have subject to gap between any two rice leafs, the quantity of vertical straight line is equal with the quantity of rice leaf collected.
Blade described in step (4) is fixed on waterproof paper are as follows: is taken 1 rice leaf, is pruned vacuum side of blade with blade Vein convex portion, makes vein and blade in a plane, coats solid gum in vacuum side of blade, blade is placed on one vertically and erects On straight straight line, and the vein of blade is corresponding in the vertical straight line position, and blade is attached on waterproof paper, is on one side attached to blade anti- On water paper, the cotton paper towel soaked on one side with clear water all covers the blade surface posted, and all blades so operate one by one It is fixed, after all blades are fixed, take cotton paper towel away, the moisture sassafras on each blade surface is done with toilet paper.
Compared with prior art, the beneficial effects of the present invention are:
1, of good preservation effect.The present invention is that rice leaf is in fresh-keeping state from whole process of taking pictures is sampled to, and is prevented The quick dehydration of rice leaf is stopped and has dried up eclipsed, it is ensured that the rice leaf sample photo of acquisition is fresh and alive, true to nature.
2, equidistant vertical straight line is drawn on waterproof paper, by the way that blade vein is corresponding in vertical straight line position, blade It is attached on waterproof paper, it is ensured that all leaf blade specimen marshallings are coordinated.
3, simple and efficient to handle.The present invention obtains photo using scanner, and the waterproof paper for fixing blade need to will be only housed Valve bag be put on scanner, set required pixel, save format, the elements such as picture color, click scan, operation Personnel are not limited by shooting technology.
4, the vein of rice leaf back side protrusion is scabbled, is conducive to for entire leveling blade being laid on paper, thus Blade is improved to take pictures aesthetic measure.
Detailed description of the invention
Fig. 1: leaf long 280mm, leaf width 10mm or so feel the photograph that 9 rice leaf scanner scannings of bacterial leaf-blight obtain Piece.
Fig. 2: leaf long 200mm, leaf width 10mm or so feel the photograph that 14 rice leaf scanner scannings of bacterial leaf-blight obtain Piece.
Fig. 3: leaf long 400mm, leaf width 10mm or so feel the photograph that 14 rice leaf scanner scannings of bacterial leaf-blight obtain Piece.
Specific embodiment
Case is embodied below, the invention will be further described, in each embodiment without specified otherwise be conventional method.
Embodiment 1
Leaf long 280mm, leaf width 10mm or so feel the photographic method of 9 rice leafs of bacterial leaf-blight:
(1) preparation of consumptive material: including solid gum, A4 size white waterproof paper (long 297mm* wide 210mm), 400mm long Ruler, Pencil with 2B hardness, blade, test tube (diameter 20mm* high 200mm), the cotton paper towel of big specification, valve bag (long 220mm* wide 320mm), scanner (can generally scan A4 paper), common sanitary paper towel and clear water.Consumables associated therewith is commercially available.
(2) rice leaf acquisition and keeping: lower 9 rice leafs will be acquired from living body rice plant and are put into togerther one Carry out fresh-keeping in test tube equipped with clear water, 9 rice leafs are put into togerther a test tube, and blade will not be floated up easily, should The test tube of specification is put 30 rice leafs and is ok.Clear water floods the lower part of all rice leafs and does not flood the position for having scab It sets, it is fresh-keeping that the lower part that clear water only floods rice leaf can reach water suction, it is not necessary to all flood rice leaf, as feeling bacterial leaf spot The blade of disease, scab is all on top, and after blade floods completely, germ can melt in water, and scab can fog, just as having moved back face Color is the same, and such blade can be distorted instead.
(3) position of fixed blade is made on waterproof paper: waterproof paper is placed vertically, from waterproof paper left side 10mm initial line, is used Ruler and pencil prevent it is waterborne draw the equal a plurality of vertical straight line of spacing distance, spacing distance size is adjacent to be placed on vertically Have subject to gap between any two blades collected in two vertical straight lines, the quantity of vertical straight line and leaf collected The quantity of piece is equal, the length of straight line and the equal length of blade, multiple cotton paper towel is put into spare in clear water.
(4) blade is fixed on waterproof paper: taking 1 blade, vacuum side of blade vein convex portion is gently pruned with blade, is made Vein and blade coat solid gum in vacuum side of blade, blade is placed on vertically in a vertical straight line, and leaf in approximately the same plane The vein of piece is corresponding to be attached to blade on waterproof paper in the position of the vertical straight line, takes out the cotton paper towel being put into clear water, twists and go Part clear water makes it not drip as the cotton paper towel of clear water wetting, and blade is attached on waterproof paper on one side, and (immediately) is with clearly on one side The cotton paper towel of water wetting all covers the blade surface posted, and so operation is fixed (if a Zhang Qing one by one for all blades The cotton paper towel of water wetting can be by all blade all standings, the cotton paper towel that can be only soaked with a clear water, if a clear water profit Wet cotton paper towel cannot be by all blade all standings, the cotton paper towel that can be soaked with multiple clear water, as long as often posting a blade The cotton paper towel soaked immediately with clear water all covers the blade surface, so that the blade not dehydration), it is solid to all blades After fixed, take cotton paper towel away, the moisture on each blade surface is dried and (blotted) with toilet paper, the waterproof of blade will be fixed Paper is packed into valve bag and saves.
(5) valve bag equipped with the waterproof paper for fixing blade is put in scanning on scanner and obtains photo.Photo is shown in figure 1。
Valve bag equipped with the waterproof paper for fixing blade is put on scanner and is scanned, rather than is taken out from valve bag Fix blade waterproof paper put scanning on the scanner can or dehydration impaired to avoid blade.
Embodiment 2
Leaf long 200mm, leaf width 10mm or so feel the photographic method of 14 rice leafs of bacterial leaf-blight:
In addition to the rice leaf under acquiring from living body rice plant is different, remaining operation is same as Example 1.Photo is shown in Fig. 2.
Embodiment 3
Leaf long 400mm, leaf width 10mm or so feel the photographic method of 14 rice leafs of bacterial leaf-blight:
(1) preparation of consumptive material: including solid gum, white waterproof paper (long 410mm* wide 300mm), the ruler of 400mm long, 2B Pencil, blade, test tube (diameter 20mm* long 200mm), the cotton paper towel of big specification, valve bag (long 450mm* wide 350mm), scanning Instrument (can generally scan A4 paper), common sanitary paper towel and clear water.Consumables associated therewith is commercially available.
(2) rice leaf acquisition and keeping: 14 rice leafs under acquiring from living body rice plant are put into togerther Carry out fresh-keeping in one test tube equipped with clear water, clear water floods the lower part of all rice leafs and do not flood the position for having scab.
(3) position of fixed blade is made on waterproof paper: waterproof paper is placed vertically, from waterproof paper left side 0.5cm initial line, is used Ruler and pencil drawn on waterproof paper spacing distance be 20mm 14 root longs be 400mm vertical straight line, while by multiple Cotton paper towel is put into spare in clear water.
(4) blade is fixed on waterproof paper: taking 1 rice leaf, vacuum side of blade vein lug boss is gently pruned with blade Point, make vein and blade in approximately the same plane, coats solid gum in vacuum side of blade, blade is placed on a vertical straight line vertically On, and the vein of blade is corresponding in the position of the vertical straight line, and blade is attached on waterproof paper, the cotton paper towel in clear water is taken Out, twisting goes part clear water that it is made not drip as the cotton paper towel of clear water wetting, and blade is attached on waterproof paper on one side, (vertical on one side I.e.) the cotton paper towel soaked with clear water all covers the blade posted, all blades one by one so operation it is fixed (if one The cotton paper towel of Zhang Qingshui wetting can be by all blade all standings, the cotton paper towel that can be only soaked with a clear water a, if Zhang Qing The cotton paper towel of water wetting cannot be by all blade all standings, the cotton paper towel that can be soaked with multiple clear water, as long as often posting one The cotton paper towel that blade is soaked with clear water immediately all covers the blade surface, so that the blade not dehydration), to all leaves After piece is fixed, cotton paper towel is taken away, the moisture sassafras on each blade surface is done (blotting) with toilet paper, will fix blade Waterproof paper is packed into valve bag and saves.
(5) valve bag equipped with the waterproof paper for fixing blade is put in the scanning of scanner upper curtate, the picture of acquisition is adopted With related software split.Photo is shown in Fig. 3.
Above-described embodiment is according to the case study on implementation under the premise of length of blade, waterproof paper area and scanner size, if leaf Not in case study on implementation range, operator can be according to the above case study on implementation to the interval distance between vertical straight line for leaf length and width From being adjusted, if there are many blade quantity, it can refer to the above case study on implementation grouping and take pictures.For the ordinary skill of the art Personnel can also make several improvement and supplement under the premise of not departing from the present invention, these are improved and supplement should be regarded as this hair Bright protection scope.

Claims (5)

1. a kind of rice leaf photographic method, which comprises the following steps: (1) preparation of consumptive material;(2) rice leaf Acquisition and keeping;(3) position of fixed blade is made on waterproof paper;(4) blade is fixed on waterproof paper, while being packed into self-styled Bag saves;(5) valve bag equipped with the waterproof paper for fixing blade is put in scanning on scanner and obtains photo.
2. rice leaf photographic method as described in claim 1, which is characterized in that the preparation packet of consumptive material described in step (1) It includes and prepares solid gum, waterproof paper, ruler, pencil, blade, test tube, cotton paper towel, toilet paper, valve bag, scanner and clear water.
3. rice leaf photographic method as described in claim 1, which is characterized in that the acquisition of rice leaf described in step (2) With keeping are as follows: will be put into the test tube equipped with clear water from the rice leaf that living body rice plant collects carry out it is fresh-keeping.
4. rice leaf photographic method as described in claim 1, which is characterized in that step is made on waterproof paper described in (3) The position for making fixed blade is a plurality of vertical straight line for drawing distance at equal intervals on waterproof paper with ruler and pencil, spacing distance Size, which is subject between any two rice leafs collected being placed in adjacent two vertical straight lines vertically, gap, erects The quantity of straight straight line is equal with the quantity of rice leaf collected.
5. rice leaf photographic method as described in claim 1, which is characterized in that blade described in step (4) is fixed on On waterproof paper are as follows: take 1 rice leaf, prune vacuum side of blade vein convex portion with blade, keep vein and blade flat at one Solid gum is coated in vacuum side of blade in face, and blade is placed on vertically in a vertical straight line, and the vein of blade is corresponding vertical at this Blade is attached on waterproof paper by linear position, and blade is attached on waterproof paper on one side, on one side should with the cotton paper towel that clear water soaks The blade surface posted all covers, and all blades one by one so fix by operation, after all blades are fixed, takes cotton paper towel away, The moisture sassafras on each blade surface is done with toilet paper.
CN201910519489.6A 2019-06-17 2019-06-17 A kind of rice leaf photographic method Pending CN110248039A (en)

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Application Number Priority Date Filing Date Title
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113916887A (en) * 2021-10-15 2022-01-11 广东省科学院南繁种业研究所 Sugarcane leaf photographing method

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102809357A (en) * 2012-08-17 2012-12-05 华中农业大学 Automatic measuring and analyzing instrument for crop leaf area information
CN103139436A (en) * 2013-03-11 2013-06-05 安徽科技学院 Technique using platform type scanner to save images of plant samples
CN204244334U (en) * 2014-12-24 2015-04-01 沈阳农业大学 Based on Embedded sweep type crops leaf diseases hierarchical detection device
CN105644242A (en) * 2014-11-13 2016-06-08 张琪 Leaf-vein shaving knife for leaf carving
CN108120402A (en) * 2017-12-27 2018-06-05 广西壮族自治区林业科学研究院 A kind of method that plant leaf area is measured based on Photoshop softwares
CN109325630A (en) * 2018-10-12 2019-02-12 南京农业大学 Rice production prediction method under a kind of high temperature stress based on morphological parameters

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102809357A (en) * 2012-08-17 2012-12-05 华中农业大学 Automatic measuring and analyzing instrument for crop leaf area information
CN103139436A (en) * 2013-03-11 2013-06-05 安徽科技学院 Technique using platform type scanner to save images of plant samples
CN105644242A (en) * 2014-11-13 2016-06-08 张琪 Leaf-vein shaving knife for leaf carving
CN204244334U (en) * 2014-12-24 2015-04-01 沈阳农业大学 Based on Embedded sweep type crops leaf diseases hierarchical detection device
CN108120402A (en) * 2017-12-27 2018-06-05 广西壮族自治区林业科学研究院 A kind of method that plant leaf area is measured based on Photoshop softwares
CN109325630A (en) * 2018-10-12 2019-02-12 南京农业大学 Rice production prediction method under a kind of high temperature stress based on morphological parameters

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113916887A (en) * 2021-10-15 2022-01-11 广东省科学院南繁种业研究所 Sugarcane leaf photographing method

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