CN113916887A - Sugarcane leaf photographing method - Google Patents
Sugarcane leaf photographing method Download PDFInfo
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- CN113916887A CN113916887A CN202111204167.6A CN202111204167A CN113916887A CN 113916887 A CN113916887 A CN 113916887A CN 202111204167 A CN202111204167 A CN 202111204167A CN 113916887 A CN113916887 A CN 113916887A
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- leaf
- sugarcane
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- sugarcane leaves
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- 240000000111 Saccharum officinarum Species 0.000 title claims abstract description 98
- 235000007201 Saccharum officinarum Nutrition 0.000 title claims abstract description 98
- 238000000034 method Methods 0.000 title claims abstract description 22
- 210000005069 ears Anatomy 0.000 claims abstract description 52
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 36
- 239000002390 adhesive tape Substances 0.000 claims abstract description 16
- 238000004321 preservation Methods 0.000 claims abstract description 3
- 210000003462 vein Anatomy 0.000 claims abstract description 3
- 230000003020 moisturizing effect Effects 0.000 claims description 15
- 240000006409 Acacia auriculiformis Species 0.000 claims description 2
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 11
- 239000000853 adhesive Substances 0.000 description 7
- 230000001070 adhesive effect Effects 0.000 description 7
- 239000004744 fabric Substances 0.000 description 5
- 238000005286 illumination Methods 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000124033 Salix Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000000473 mesophyll cell Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 230000005068 transpiration Effects 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N2021/8466—Investigation of vegetal material, e.g. leaves, plants, fruits
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a sugarcane leaf photographing method, which comprises the following steps: taking down sugarcane leaves in the field, wherein the length of the sugarcane leaves from leaf ears to leaf tails is 1-1.5 m, and carrying out moisture preservation treatment on the leaf ears; after the sugarcane leaves are transported back to a room, the leaf ear parts are soaked in water for no more than 24 hours, the water submerges the leaf ear parts for 3-12 cm to keep the sugarcane leaves in a spread state, the leaf ears are cut off after moisture in the leaf ear parts is sucked dry and are placed on a photographed background, three sections of double-sided adhesive tape vertical to leaf veins are respectively stuck to the head part, the middle part and the tail part of the back of the sugarcane leaves, so that the sugarcane leaves are fixed on the photographed background and are kept in the spread state; and (6) taking a picture. The sugarcane leaf unfolding device enables the sugarcane leaves before photographing to be kept in the unfolding state within 24 hours, can clearly shoot the sugarcane leaves which are completely unfolded, and is beneficial to the technical research of the sugarcane leaves.
Description
Technical Field
The invention belongs to the field of biotechnology research, and relates to a sugarcane leaf photographing method.
Background
The leaf is the most important component of the plant, and the green flat body with a large surface area can facilitate the gas exchange and the light energy absorption between mesophyll cells and the outside, so that the photosynthesis and the transpiration can be realized. The sugarcane leaves are plant leaves which are rich in resources and low in price, contain various antioxidant components such as flavonoids, polyphenols and the like, and are important sources of exogenous antioxidant substances for human bodies. Sugarcane top is also a high-quality organic fertilizer source which can be utilized on the spot.
Therefore, the research on the sugarcane leaves is of great significance, and among them, the selection of proper sugarcane leaves for research is particularly important through observation of the shapes, colors and the like of the sugarcane leaves, and in later published papers and periodicals, the requirements on photographs of the sugarcane leaves are very strict, but the sugarcane leaves curl a few minutes after being collected and are not easy to fix on a photographic background, so that the research on the sugarcane leaves is very unfavorable. The invention provides a sugarcane leaf photographing method, which can keep sugarcane leaves in a unfolding state within 24 hours before photographing and can clearly shoot the sugarcane leaves which are completely unfolded.
The invention content is as follows:
the invention aims to provide a photographing method of sugarcane leaves, which can obtain a picture of the sugarcane leaves with good stretching.
The technical scheme for achieving the aim comprises the following steps.
A photographing method of sugarcane leaves comprises the following steps:
(1) taking down sugarcane leaves in the field, wherein the length of the sugarcane leaves from leaf ears to leaf tails is 1-1.5 m, and carrying out moisture preservation treatment on the leaf ears;
(2) after the sugarcane leaves are transported back to a room, the leaf ear parts are soaked in water for no more than 24 hours, the water submerges the leaf ear parts for 3-12 cm to keep the sugarcane leaves in a spread state, the leaf ears are cut off after moisture in the leaf ear parts is sucked dry and are placed on a photographed background, three sections of double-sided adhesive tape vertical to leaf veins are respectively stuck to the head part, the middle part and the tail part of the back of the sugarcane leaves, so that the sugarcane leaves are fixed on the photographed background and are kept in the spread state;
(3) and (6) taking a picture.
In some embodiments, the length of the double-sided adhesive tape does not exceed the width of the leaf width of the head, middle and tail of the corresponding sugarcane leaf.
In some of these embodiments, the water in step (2) is 5-12 cm above the lobe segment, more preferably, the water in step (2) is 5-10 cm above the lobe segment.
In some embodiments, the method further comprises attaching double-sided adhesive to the back of the sugarcane leaf at the crimps.
In some embodiments, the sugarcane leaf blade described in step (1) is taken from the sugarcane variety willow city 01-122 tillering stage.
In some embodiments, the moisturizing treatment of the leaf ears in step (1) is to moisturize the leaf ears by placing a moisturizing bag on the leaf ears.
In some of these embodiments, the lengths of the double-sided adhesive tapes are 1-6 cm, respectively, and more preferably, the lengths of the double-sided adhesive tapes are 2-5 cm, respectively.
In some of these embodiments, the auris sativa is immersed in water in step (2) for a period of time ranging from 5 to 24 hours, more preferably from 6 to 21 hours.
In some of these embodiments, the photographed background in step (3) is black waterproof paper.
The sugarcane leaves are curled within a few minutes after being collected and cannot be easily fixed on a photographing background, and in long-term experience, the inventor of the application finds that if the sugarcane leaves picked from a field are subjected to moisturizing treatment and transported back to the room, leaf ears are placed in water for 3-12 centimeters (particularly preferably more than 5 centimeters), the sugarcane leaves are not easy to shrink, so that the sugarcane leaves before photographing are kept in a unfolded state as far as possible within 24 hours without being remarkably shrunk, double-sided adhesive instead of transparent adhesive is selected for adhering the sugarcane leaves, photographed pictures can be prevented from being easily reflected, the sugarcane leaves are completely displayed, the whole pictures are cleaner and tidier, and the research on the sugarcane leaves can provide good assistance.
Drawings
FIG. 1: the camera of the sugarcane leaf in comparative example 1 takes a picture.
FIG. 2: the camera of the sugarcane leaf in example 1 takes a picture.
Fig. 3 is a photograph taken by a camera of a sugarcane leaf in comparative examples 1 to 3, wherein 2cm represents comparative example 2, 5cm represents comparative example 1, 12cm represents comparative example 3, and ck represents that the sugarcane leaf is not processed, i.e., the leaf is collected and not discharged, but is directly placed on a table.
Detailed Description
The following examples of the present invention are experimental methods without specifying specific conditions, generally according to conventional conditions, or according to conditions recommended by the manufacturer. The various chemicals used in the examples are commercially available.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.
The terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusions. For example, a process, method, apparatus, product, or device that comprises a list of steps is not limited to only those steps or components listed, but may alternatively include other steps or components not listed, or inherent to such process, method, product, or device.
The "plurality" referred to in the present invention means two or more. "and/or" describes the association relationship of the associated objects, meaning that there may be three relationships, e.g., a and/or B, which may mean: a exists alone, A and B exist simultaneously, and B exists alone. The character "/" generally indicates that the former and latter associated objects are in an "or" relationship.
In order that the invention may be more fully understood, reference will now be made to the following description. The present invention may be embodied in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
Example 1
(1) Collecting 10 leaves of sugarcane variety Liucheng 01-122 at tillering stage in field, wherein the length of the obtained sugarcane leaves is about 1.0-1.5 m from leaf ear parts (extensions of leaf edges at two sides of the joint of a leaf sheath and the leaves, which are in a shape of multiple ears or sickles) to leaf tails, putting the leaf ear parts into a moisturizing bag for moisturizing, and transporting the leaf ear parts back to the room (about 0.5 hour);
(2) after the sugarcane leaves are transported back to a room, the leaf ear parts are soaked in water for 6-7 hours, the water submerges 5cm of the leaf ear parts to keep the leaves unfolded, the leaf ear water is sucked by dry cloth and then immediately placed on black waterproof paper, double-sided adhesive tapes are respectively pasted on the head parts (0.1-0.15 m away from the leaf ear), the middle parts (0.5-0.7 m away from the leaf ear) and the tail parts (0.1-0.15 m away from the leaf tail) of the back surfaces of the sugarcane leaves to fix the leaves and keep the unfolded state, the length of each section of double-sided adhesive tape is 2-5 cm, and the length of each double-sided adhesive tape is not more than the leaf width of the head parts, the middle parts and the tail parts of the corresponding sugarcane leaves;
(3) and adjusting the illumination angle to take a picture.
Example 2
(1) Collecting 10 leaves of sugarcane variety Liucheng 01-122 at tillering stage in field, wherein the length of the obtained sugarcane leaves from leaf ears to leaf tails is 1.0-1.5 m, placing the leaf ears in a moisturizing bag for moisturizing, and transporting the leaves back to the room (0.5 hour);
(2) after the sugarcane leaves are transported back to the room, the leaf ears are put in water for 20-21 hours, the water submerges the leaf ears by 5cm to keep the leaves unfolded, the leaf ears are dried by dry cloth and then immediately put on black waterproof paper, double-sided adhesive tapes are respectively pasted on the head (0.1-0.15 m away from the leaf ears), the middle (0.5-0.7 m away from the leaf ears) and the tail (0.1-0.15 m away from the leaf tails) of the back sides of the sugarcane leaves to fix the leaves and keep the unfolded state, and the length of each section of double-sided adhesive tape is 1-6 cm;
(3) and adjusting the illumination angle to take a picture.
Comparative example 1
(1) Collecting 10 leaves of sugarcane variety Liucheng 01-122 at tillering stage in field, respectively obtaining sugarcane leaves with the length of 1.0-1.5 m from leaf ear to leaf tail, placing the leaf ear in a moisturizing bag for moisturizing, and transporting the leaf ear back to the room (0.5 hour);
(2) after the sugarcane leaves are transported back to the room, the leaf ears are put in water for 6-7 hours, the water submerges 5cm above the leaf ears to keep the leaves unfolded, the leaf ears are immediately put on black waterproof paper after moisture of the leaf ears is absorbed by dry cloth, and the head (0.1-0.15 m away from the leaf ears), the middle (0.5-0.7 m away from the leaf ears) and the tail (0.1-0.15 m away from the leaf tails) at the back of the sugarcane leaves are respectively stuck with transparent glue to fix the leaves and keep the unfolded state;
(3) and adjusting the illumination angle to take a picture.
Comparative example 2
(1) Collecting 10 leaves of sugarcane variety Liucheng 01-122 at tillering stage in field, wherein the length of the obtained sugarcane leaves from leaf ears to leaf tails is 1.0-1.5 m, placing the leaf ears in a moisturizing bag for moisturizing, and transporting the leaf ears back to the room (0.5 hour);
(2) after the sugarcane leaves are transported back to the room, the leaf ears are put in water for 6-7 hours, the water does not exceed 3cm beyond the leaf ears by 2cm, so as to keep the leaves unfolded, the water is absorbed by dry cloth and then immediately put on black waterproof paper, double-sided adhesive tapes are respectively pasted on the head (0.1-0.15 m away from the leaf ears), the middle (0.5-0.7 m away from the leaf ears) and the tail (0.1-0.15 m away from the leaf tails) of the back surfaces of the sugarcane leaves so as to fix the leaves and keep the unfolded state, the double-sided adhesive tapes are also pasted on the back surfaces of the shrunken parts, and the lengths of the double-sided adhesive tapes are respectively 1-6 cm;
(3) and adjusting the illumination angle to take a picture.
Comparative example 3
(1) Collecting 10 leaves of sugarcane variety Liucheng 01-122 at tillering stage in field, wherein the length of the obtained sugarcane leaves from leaf ears to leaf tails is about 1.0-1.5 m, placing the leaf ears in a moisturizing bag for moisturizing, and transporting the leaves back to the room (0.5 hour);
(2) after the sugarcane leaves are transported back to the room, the leaf ears are put in water for 20-21 hours, the water submerges the leaf ears by 12 centimeters to keep the leaves unfolded, the water is absorbed by dry cloth and then immediately put on black waterproof paper, double-sided adhesive is respectively pasted on the head (0.1-0.15 meter from the leaf ears), the middle (0.5-0.7 meter from the leaf ears) and the tail (0.1-0.15 meter from the leaf tails) of the back surfaces of the sugarcane leaves to fix the leaves and keep the unfolded state, the length of each section of double-sided adhesive is 1-6 centimeters, the back surfaces of the shrunken parts are also pasted with the double-sided adhesive, and the length of the double-sided adhesive is 1-6 centimeters;
(3) and adjusting the illumination angle to take a picture.
Results of the experiment
The photo taken in example 1 is shown in fig. 2, when the leaf ear is put in water for 5-7 hours and the water is 5cm over the leaf ear, the sugarcane leaf is not shriveled, the sugarcane leaf can be smoothly stuck by double-sided adhesive tape, the shot photo is not easy to reflect light, the sugarcane leaf is completely displayed, and the whole photo is very clean and tidy. In example 2, the sugarcane leaves did not shrink when the leaf ears were placed in water for 20-21 hours and the water was 5cm over the leaf ears. The photograph taken in comparative example 1 is shown in fig. 1, and the sugarcane leaf is stuck with a transparent tape which not only reflects light but also shields a part of the leaf, although the leaf is also properly treated and the unfolded state of the leaf is good. In comparative example 2, when the sugarcane leaves were placed in water and the water was 2cm over the leaf ears, some of the sugarcane leaves were shriveled. In comparative example 3, when the leaf ears were put in water for 20 to 21 hours and the water was over 12cm of the leaf ears, the sugarcane leaves were not shrunken and the photographing effect was also good.
Therefore, the sugarcane leaf blade ear parts are creatively placed in water, and when the water is 3cm above the sugarcane leaf blade ears and especially 5-12 cm below the sugarcane leaf blade ears, the sugarcane leaf blades can still be enabled not to shrink in a large area even if the sugarcane leaf blade leaves are placed for 20 hours and cannot be photographed in time, then the double-sided adhesive tape is selected to be pasted on the sugarcane leaf blades, the photographed photos can still achieve that the sugarcane leaf blades are completely unfolded, the photographed photos are not reflective, the whole photos are clean and tidy, and the sugarcane leaf blade is well helped for researching the sugarcane leaf blades.
While the preferred embodiments of the present invention have been described in detail, it will be understood by those skilled in the art that the invention is not limited thereto, and that various changes and modifications may be made without departing from the spirit of the invention, and the scope of the appended claims is to be accorded the full range of equivalents.
Claims (10)
1. A photographing method of sugarcane leaves is characterized by comprising the following steps:
(1) taking down sugarcane leaves in the field, wherein the length of the sugarcane leaves from leaf ears to leaf tails is 1-1.5 m, and carrying out moisture preservation treatment on the leaf ears;
(2) after the sugarcane leaves are transported back to a room, the leaf ear parts are soaked in water for no more than 24 hours, the water submerges the leaf ear parts for 3-12 cm to keep the sugarcane leaves in a spread state, the leaf ears are cut off after moisture in the leaf ear parts is sucked dry and are placed on a photographed background, three sections of double-sided adhesive tape vertical to leaf veins are respectively stuck to the head part, the middle part and the tail part of the back of the sugarcane leaves, so that the sugarcane leaves are fixed on the photographed background and are kept in the spread state;
(3) and (6) taking a picture.
2. The photographing method of claim 1, wherein the length of the double-sided tape does not exceed the width of the leaf width of the head, the middle and the tail of the corresponding sugarcane leaf.
3. The photographing method according to claim 1 or 2, wherein the water in step (2) is 5-12 cm over the auricle part, preferably, the water in step (2) is 5-10 cm over the auricle part.
4. The photographing method according to claim 1 or 2, further comprising attaching a double-sided tape to the back of the shrunken part of the sugarcane leaf.
5. The photographing method according to claim 1, wherein the sugarcane leaf in the step (1) is taken from the sugarcane variety Liucheng 01-122 tillering stage.
6. The photographing method according to claim 1, wherein the moisturizing treatment is performed on the leaf ears in step (1) by placing a moisturizing bag on the leaf ears to moisturize the leaf ears.
7. The photographing method according to claim 2, wherein the lengths of the double-sided tapes are 1 to 6 cm, respectively.
8. The photographing method according to claim 7, wherein the lengths of the double-sided tapes are 2 to 5cm, respectively.
9. The photographing method according to any one of claims 1 to 8, wherein the auris foliae is immersed in water for 5 to 24 hours in the step (2).
10. The photographing method according to any one of claims 1 to 8, wherein the background of the photographing in step (3) is black waterproof paper.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111204167.6A CN113916887A (en) | 2021-10-15 | 2021-10-15 | Sugarcane leaf photographing method |
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| CN202111204167.6A CN113916887A (en) | 2021-10-15 | 2021-10-15 | Sugarcane leaf photographing method |
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| CN113916887A true CN113916887A (en) | 2022-01-11 |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2466351C1 (en) * | 2011-06-08 | 2012-11-10 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Поволжский государственный технологический университет" | Method of measuring surface area of leaves of woody plants |
| CN110248039A (en) * | 2019-06-17 | 2019-09-17 | 云南省农业科学院生物技术与种质资源研究所 | A kind of rice leaf photographic method |
-
2021
- 2021-10-15 CN CN202111204167.6A patent/CN113916887A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2466351C1 (en) * | 2011-06-08 | 2012-11-10 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Поволжский государственный технологический университет" | Method of measuring surface area of leaves of woody plants |
| CN110248039A (en) * | 2019-06-17 | 2019-09-17 | 云南省农业科学院生物技术与种质资源研究所 | A kind of rice leaf photographic method |
Non-Patent Citations (1)
| Title |
|---|
| 许美玲: "活动式田间拍摄背景的设计及烟草种质特性照片拍摄技术", 植物遗传资源学报, vol. 09, no. 04, 16 December 2008 (2008-12-16), pages 540 - 544 * |
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