CN109060800A - A kind of fish-egg, larval morphometrics method for drafting - Google Patents
A kind of fish-egg, larval morphometrics method for drafting Download PDFInfo
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- CN109060800A CN109060800A CN201810632792.2A CN201810632792A CN109060800A CN 109060800 A CN109060800 A CN 109060800A CN 201810632792 A CN201810632792 A CN 201810632792A CN 109060800 A CN109060800 A CN 109060800A
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Abstract
The present invention relates to a kind of fish-eggs, larval morphometrics method for drafting, stage of development judgement and preliminary Identification of Species are carried out to fish-egg and larva and juvenile based on biological anatomical lens, shooting processing is carried out by morphological feature of the high resolution microscope to fish-egg and larva and juvenile type, then by fish-egg, each position shooting picture of larva and juvenile carries out folded figure processing with Photoshop software, final export has the fish-egg, the picture of all clear morphological features of larva and juvenile, fish-egg by the way that every is taken pictures again, larva and juvenile carries out molecular biology and handles determining species, then picture is carried out with species corresponding, by by photo print, picture is copied on template to be handled, it is put into scanner and is scanned processing, finally obtain fish-egg, the morphological feature and developmental morphology figure of larva and juvenile.The present invention overcomes previous fish-egg, larval morphometrics features to draw the expert's just drawable deficiency for needing long campaigns to paint and be engaged in fish feature related fields, it is shown that its quickly, accurately and efficiently advantage.
Description
Technical field
The present invention relates to fishery fields, and in particular to a kind of fish-egg, larval morphometrics method for drafting.
Background technique
The identification of early development stage fish species is the basis of fish early life history research.Traditional fish early stage provides
Source category discrimination method is mainly based on early stage individual morphology feature, using variety classes early development stage various denumerable
Difference in character, metric characters and description character carries out different types of differentiation.It is external for larva and juvenile research history
The research of larva and juvenile is carried out very early, or even can be traced to the 80s and 90s in 19th century, focus primarily upon fish spawning habit and
The research of embryonic development.The developed countries such as America and Europe, Japan are due to long-term in terms of fish early life history related discipline research
Accumulation possesses huge fish earlier life research scientific research personnel troop, forms complete fish early stage resource form abundant
The materials of identification.Due to the dynamic change of early life history stage individual morphology method, characteristic of division is limited, particularly some relationships are closed
It is in close type, morphological differences is extremely subtle, and there are the subjective judgements of error and sorter for qualification result.Thus with warp
Allusion quotation morphological method must be familiar with the young morphological feature of each section, genus and species, and the grasp different developmental phases individual morphology of system becomes
Change, and the basis compared according to it as form between variety classes, often the expert of the long campaigns area research just can be carried out
Identification.In recent years, it with the fast development of Protocols in Molecular Biology, is provided to solve fish early development stage classification problem
A kind of new approach.This method utilizes higher genetic homogeneity between species population, obtains inter-species by known type sample
Diagnostic characteristics, and using these features to sample to be detected carry out according to identification.The method overcome main in Morphological Identification
The strong disadvantage of the property seen also has many advantages, such as high sensitivity, reproducible, answers extensively in fish early stage resource category is identified
With identification based on biological anatomical lens to fish-egg and larva and juvenile type, by carrying out individual morphology to fish-egg and larva and juvenile type
Developmental morphology figure is observed and drawn to feature, and previous morphological feature drafting generally requires long campaigns drawing and is engaged in fish
The expert of feature related fields can just draw out the morphological feature figure of fish early stage resource, and process is time-consuming and laborious and efficiency is lower.
Summary of the invention
In view of the above-mentioned problems, being solved existing the object of the present invention is to provide a kind of fish-egg, the form method for drafting of larva and juvenile
The deficiency that the waters such as ocean, fresh water fish-egg, larval morphometrics feature are drawn.The present invention to achieve the above object, using following skill
Art scheme:
A kind of fish-egg, larval morphometrics method for drafting, which comprises the following steps:
(1) sample of the acquisition of fish-egg and larva and juvenile sample, acquisition is fixed using 5% formalin solution；
(2) fish-egg chosen and larva and juvenile are carried out to stage of development judgement and preliminary Identification of Species under biological anatomical lens；
(3) sample of preliminary Identification of Species is put under high resolution microscope and processing of taking pictures is carried out to fish-egg, larva and juvenile；
(4) folded figure processing is carried out to shooting picture with photo handling software, exports picture；
(5) fish-egg for processing of taking pictures in step 3, larva and juvenile are subjected to molecular biology processing；
(6) fish-egg of the type determined after molecular biology is handled in step 5 and larva and juvenile and step 4 are handled
Picture is corresponded to；Determine the morphological feature of this stage of development corresponding to this kind of fish-egg, larva and juvenile.
Preferably, in the step 1, ocean fish-egg, larva and juvenile use aperture to swim for large size made of 0.505mm sieve Juan
Biology carries out surface layer horizontal trawl and vertical trawl net；The acquisition of the fish-egg, larva and juvenile of inland river is quantified using brailer
Acquisition, while auxiliary qualitative collection is carried out using Jianggang net.
Preferably, the fish-egg of the step 3 is taken pictures in processing, the shape of ovum, ovum diameter, egg membrane position, oily ball quantity and straight
Diameter, yolk size, idiosome form, pigment shape and distributed areas, idiosome muscle segment, the shape of each major organs and position carry out
It takes pictures；In the processing of taking pictures of larva and juvenile, take pictures first to the form of larva and juvenile, record body is long, before long, eye diameter, anus away from,
Body height, muscle segment number, gill cover spine, dentition, the shape and distributing position of anus position and pigment；Then larva and juvenile fin ray is carried out
Shooting, includes the growth and decline of fin film, the differentiation and developmental state of each fin, the number of spine and fin ray, fin ray size, the position of fin ray
It sets, the development condition and its morphological feature of tail fin bone.
Preferably, in the step 4: using Photoshop software that the shooting picture of fish-egg, larva and juvenile is each with carrying out
Figure processing is folded at clear position, exports the picture with this fish-egg, the clear morphological feature of larva and juvenile.
Preferably, young to fish-egg and son using the Cytochrome c oxidase I gene (COI) of mitochondria in the step 5
Fish respective segments carry out amplification sequencing, and what is be then compared with COI sequence database determines fish-egg and larva and juvenile
Type.
Preferably, include the steps that in the method for drafting as follows: by fish-egg, larva and juvenile photo print；By template
It is layered on picture, fish-egg, larval morphometrics feature is copied on template, then scanning saves as picture, determines this kind
Morphological feature corresponding to class fish-egg, larva and juvenile and developmental morphology figure.
The present invention is based on biological anatomical lens to carry out stage of development judgement and preliminary Identification of Species to fish-egg and larva and juvenile, passes through
High resolution microscope carries out shooting processing to the morphological feature of fish-egg and larva and juvenile type, finally with Photoshop software into
Figure processing is folded at each position of row fish-egg, larva and juvenile, and final export has the picture of this fish-egg, all morphological features of larva and juvenile, so
It is handled again by template arriving tracing piece afterwards, finally obtains developmental morphology figure, overcome previous fish-egg, larval morphometrics
Feature draws the expert for needing long campaigns to paint and be engaged in fish feature related fields just drawable deficiency, have quickly,
Accurately and efficiently advantage.
Figure of description
The development depiction of Fig. 1 decapterus maruadsi early forms；
Fig. 2 yellowfin tuna early forms develop depiction；
The recessed shoulder Scad early forms of Fig. 3 rouge eye develop depiction；
Fig. 4 Tiao Wen La early forms develop depiction.
Specific embodiment
Technical solution of the present invention is described in detail below by specific embodiment, but the scope of the present invention
It is not restricted by the embodiments.The present invention is a kind of fish-egg, larval morphometrics method for drafting, using following steps:
(1) acquisition of fish-egg and larva and juvenile sample, the acquisition of ocean fish-egg, larva and juvenile is according to " standard of marine survey "
(GB127631-7-91) use aperture for macroplankton net (network interface internal diameter 80cm, length made of 0.505mm sieve Juan
270cm, network interface area are 0.5m2) according to the progress surface layer horizontal trawl of pre-set investigation station site and vertical trawl net；Inland
The acquisition of the fish-egg, larva and juvenile in river nets the imitative brailer changed using Bongo and carries out quantitative collection, while use is according to fisherman's well rope
The Jianggang net of net restructuring carries out auxiliary qualitative collection, and the sample of acquisition is fixed using 5% formalin solution；
(3) fish-egg chosen and larva and juvenile are carried out to stage of development judgement and preliminary type under laboratory biological anatomical lens
Identification；
(4) sample of preliminary Identification of Species is put under high resolution microscope and processing of taking pictures is carried out to fish-egg, larva and juvenile:
Fish-egg is taken pictures in processing, the shape of ovum, ovum diameter, egg membrane position, oily ball quantity and diameter, yolk size, idiosome form, pigment shape
Shape and distributed areas, idiosome muscle segment, the shape of each major organs and position are taken pictures；It is first in the processing of taking pictures of larva and juvenile
First take pictures to the form of larva and juvenile, record body is long, before long, eye diameter, anus away from, body height, muscle segment number, gill cover spine, dentition, anus
The shape and distributing position of door position and pigment；Then the shooting for carrying out larva and juvenile fin ray, includes the growth and decline of fin film, each fin
Differentiation and developmental state, the number of spine and fin ray, fin ray size, the position of fin ray, the development condition and its form of tail fin bone
Feature；
(5) the shooting picture of fish-egg, larva and juvenile is subjected to each position with Photoshop software and folds figure processing, export tool
There is the picture of all clear morphological features of this fish-egg, larva and juvenile；
(6) fish-egg, the larva and juvenile then every taken pictures carry out molecular biology processing, utilize the cell color of mitochondria
Plain c oxidizing ferment I gene (COI) carries out amplification sequencing to fish-egg and larva and juvenile respective segments, then carries out with COI sequence database
The method of analysis is compared to determine the type of fish-egg and larva and juvenile；
(7) by every fish after molecular biology is handled determine type and front Photoshop processing picture into
Row corresponds to, that is, determines the morphological feature of this stage of development corresponding to this kind of fish-egg, larva and juvenile；
(8) by fish-egg, the final photo print of larva and juvenile to A4 paper；Printed A4 paper is placed on desktop, being specifically used to
It copies and the template of tracing is layered on A4 paper；Fish-egg, larval morphometrics feature are copied on template, sketch the contours fish
The morphological feature at each position of ovum, larva and juvenile；
(11) template copied is put into scanner and is scanned processing, the picture scanned is imported into computer, from
And determine morphological feature and developmental morphology figure corresponding to the type fish-egg, larva and juvenile；
(12) fish-egg and larva and juvenile of stage of development judgement and preliminary Identification of Species will be carried out under laboratory biological anatomical lens
It is selected, primarily determines and each type different growing periods are subjected to processing of taking pictures, then every fish-egg, larva and juvenile are carried out
State step process.
Finally it should be noted that above example is only with illustrating technical solution of the present invention, rather than model is protected to the present invention
The limitation enclosed, although explaining in detail referring to preferred embodiment to the present invention, those skilled in the art should be managed
Solution, can be with modification or equivalent replacement of the technical solution of the present invention are made, without departing from the reality of the invention technical solution
Matter and range.
Claims (6)
1. a kind of fish-egg, larval morphometrics method for drafting, which comprises the following steps:
(1) sample of the acquisition of fish-egg and larva and juvenile sample, acquisition is fixed using 5% formalin solution；
(2) fish-egg chosen and larva and juvenile are carried out to stage of development judgement and preliminary Identification of Species under biological anatomical lens；
(3) sample of preliminary Identification of Species is put under high resolution microscope and processing of taking pictures is carried out to fish-egg, larva and juvenile；
(4) folded figure processing is carried out to shooting picture with photo handling software, exports picture；
(5) fish-egg for processing of taking pictures in step 3, larva and juvenile are subjected to molecular biology processing；
(6) picture for handling the fish-egg of the type determined after molecular biology is handled in step 5 and larva and juvenile and step 4
It is corresponded to；Determine the morphological feature of this stage of development corresponding to this kind of fish-egg, larva and juvenile.
2. fish-egg according to claim 1, larval morphometrics method for drafting, it is characterised in that: in the step 1, ocean
Fish-egg, larva and juvenile use aperture to carry out surface layer horizontal trawl and vertical trawl net for macroplankton made of 0.505mm sieve Juan；
The acquisition of the fish-egg, larva and juvenile of inland river carries out quantitative collection using brailer, while carrying out assisting qualitative adopt using Jianggang net
Collection.
3. fish-egg according to claim 1, larval morphometrics method for drafting, it is characterised in that: the fish-egg of the step 3 is clapped
According in processing, the shape of ovum, ovum diameter, egg membrane position, oily ball quantity and diameter, yolk size, idiosome form, pigment shape and point
Cloth region, idiosome muscle segment, the shape of each organ and position are taken pictures；In the processing of taking pictures of larva and juvenile, first to larva and juvenile
Form take pictures, record body is long, before long, eye diameter, anus away from, body height, muscle segment number, gill cover spine, dentition, anus position and
The shape and distributing position of pigment；Then the shooting for carrying out larva and juvenile fin ray, includes the growth and decline of fin film, the differentiation of each fin and hair
Educate situation, the number of spine and fin ray, fin ray size, the position of fin ray, the development condition and its morphological feature of tail fin bone.
4. fish-egg according to claim 3, larval morphometrics method for drafting, which is characterized in that in the step 4: using
The shooting picture of fish-egg, larva and juvenile is folded figure processing with each clear position is carried out by Photoshop software, and export has this fish
The picture of the clear morphological feature of ovum, larva and juvenile.
5. fish-egg according to claim 1, larval morphometrics method for drafting, it is characterised in that: in the step 5, utilize
The Cytochrome c oxidase I gene (COI) of mitochondria carries out amplification sequencing to fish-egg and larva and juvenile respective segments, then with COI
What sequence database was compared determines the type of fish-egg and larva and juvenile.
6. fish-egg according to claim 1, larval morphometrics method for drafting, which is characterized in that include the steps that as follows:
By fish-egg, larva and juvenile photo print；Template is layered on picture, fish-egg, larval morphometrics feature are carried out on template
It copies, then scanning saves as picture, determines morphological feature and developmental morphology figure corresponding to the type fish-egg, larva and juvenile.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110909590A (en) * | 2019-09-19 | 2020-03-24 | 华东师范大学 | Method for acquiring plant morphological structure image |
| RU2779424C1 (en) * | 2021-07-19 | 2022-09-06 | Федеральное государственное автономное образовательное учреждение высшего образования "Сибирский федеральный университет" | Method for identification of sturgeon habitat using biochemical markers |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110909590A (en) * | 2019-09-19 | 2020-03-24 | 华东师范大学 | Method for acquiring plant morphological structure image |
| RU2779424C1 (en) * | 2021-07-19 | 2022-09-06 | Федеральное государственное автономное образовательное учреждение высшего образования "Сибирский федеральный университет" | Method for identification of sturgeon habitat using biochemical markers |
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