CN110240998A

CN110240998A - The closed bioreactor of the shell and tube of automatic cleaning tube wall

Info

Publication number: CN110240998A
Application number: CN201810212907.2A
Authority: CN
Inventors: 曾坚
Original assignee: Individual
Current assignee: Individual
Priority date: 2018-03-07
Filing date: 2018-03-07
Publication date: 2019-09-17

Abstract

Luminous energy microorganism pilot scale culture be unable to do without bioreactor, various reactor is each has something to recommend him but has quite a few drawbacks, the maximum drawback of closed bioreactor is: can not clean the internal attachment to shut out the light with water in closed situation, scheme used by the present invention solves the problems, such as this is: on reactor tank body, with several heavy caliber high transparency lucite tubes or tempered glass pipe, uprightly through the bottom and top of tank body, electric light source is hung in glass tube, become the bigbore light-emitting glass tube of several submergences in a liquid after energization to provide illumination to Object of Development, 2 circle cleaning brush up and down is covered on every glass tube, glass tube is cleaned with water by the floating drum driving in tank, blowing into compressed air to floating drum, then floating drum floats, to floating drum injection, then water floating drum sinks, intake and exhaust solenoid valve is controlled to open, it closes, it can be The attachment on automatic cleaning tube wall is realized in closed reactor.

Description

The closed bioreactor of the shell and tube of automatic cleaning tube wall

The invention belongs to luminous energy field of microbial culture technology:

Luminous energy microbial cultivation process is be unable to do without bioreactor (reaction facility), bioreactor (reaction facility) Multifarious, the Closed photobioreactor of indoor large is scale high efficiency culture production high-purity luminous energy microorganism Essential equipment, but do not come out so far.

Technical background

The utensil of the photosynthetic bacteria of large-scale artificial culture at present mainly has transparent plastic pail, transparent plastic bottle or transparent plastic Bag etc..The facility of culture microalgae then has open great Chi culture, open raceway pond culture, the culture of plate glass cylinder, heavy caliber Glass tube culture etc., they are each has something to recommend him but all there are many drawbacks.

The specific drawback of photosynthetic bacteria culture is: large-scale artificial culture photosynthetic bacteria, currently without capacity 1000L or more Closed large-scale bioreactor emerges, production method be nearly all by medium liquid and strain after mixing evenly, pour into In transparent plastic barrel, transparent plastic bottle or transparent polybag, it is placed under sunlight or irradiation allows it to be proliferated under light, in sun Then unavoidable night and rainy days if shining under light, using light-illuminating, then the efficiency of light energy utilization is too low, in incubation in order to It avoids catching the harmful microorganisms such as the miscellaneous algae of miscellaneous bacteria, after pouring into medium liquid and strain, bung bottle cap sack, which must cover tightly, to be tightened, In order to make photosynthetic bacteria strain suspend uniformly, it must shake by bag for several times to avoid precipitating by bucket by bottle, take a lot of work really daily Laborious inefficiency.

The outdoor open raceway pond of open great Chi or outdoor is come the specific drawback for cultivating microalgae: vulnerable to other biologies Pollution is restricted obvious, unavoidable low temperature day, rainy days and the night no light of encountering by weather it cannot be guaranteed that survive and purity In the case of then stop growing, be proliferated that slow algae solution concentration is low and harvesting is difficult.

It is using the drawbacks of plate glass cylinder or heavy caliber glass comb bioreactor culture: although being not easily susceptible to other biologies Pollution, the more outdoor open raceway pond of open great Chi or outdoor of growth rate is fast, but nevertheless suffers from weather and night outdoors No light restricts, and maximum drawback is then not open reactor cover board just not cleaning inside reactor with water, microalgae and Photosynthetic bacteria has phototaxis from birth, likes accumulation in the reactor and is attached on the Xiang Guangbi of reactor, as adhesive layer is too late When remove get deeper, light source is seriously blocked, cause Closed photobioreactor efficiency decline, such as in reaction time Opening cover board by force, the reaction was continued afterwards to clean with water, then is easy to cannot be guaranteed purity by other biological pollutions, since there are sealed shapes Internal attachment can not be cleaned under state with water, and this is difficult to the drawbacks of overcoming, therefore above-mentioned plate glass cylinder bioreactor is unable to get It promotes and applies, the example for the successful application that is not reported so far, for heavy caliber glass comb reactor because being architectural limitation, pipeline is very long Just inner wall can not be cleaned with water with utensil at all, even more can not think to clean with water halfway, the method that can be used can only be to speed up algae in pipeline The flow velocity of liquid, but produce little effect, because of attachment firm pasting, so heavy caliber glass comb reactor is similarly unable to get and pushes away Wide application, did not met the example for having been reported that successful application so far yet.

The drawbacks of different type bioreactor in order to overcome above-mentioned large-scale artificial culture photosynthetic bacteria and microalgae, The design provides the closed optical-biological reaction of shell and tube of (capacity 1000L or more) automatic cleaning tube wall attachment of a large size Device, it can be adhered to the attachment on tube wall in closed inside reactor automatic cleaning, and light is made not to be attached to object covering To improve light efficiency, its electric light source is immersed in culture liquid but does not contact with liquid, and replaces difference with can be convenient The electric light source of spectrum is that scale high efficiency manually cultivates high purity photosynthetic bacterium and microalgae reason to adapt to cultivated microorganism The bioreactor thought.

The technical solution adopted by the present invention to solve the technical problems has: one, irradiating light source from the outside and be changed to from interior Portion's irradiation；Two, using submarine pressurized water tank principle, the attachment cleaned on tube wall is driven with the floating drum of inside reactor, point is had Multiple floating drum forms and share single floating drum form.

Multiple floating drum forms: on cylindrical tank, with several heavy calibers (More than) high transparency it is organic Glass tube or tempered glass pipe, uprightly through the bottom and top of tank body, the overall image lotus rhizome crosscutting similar to a section runs through Sealing shroud sealing in position keeps its water-tight, and the incandescent lamp bulb for being linked to be string or fluorescent tube or LED are hung in glass inside pipe wall The electric light sources such as lamp become the bigbore light-emitting glass tube of several submergences in a liquid to give tank intracorporal culture pair after energization As providing illumination, electric light source is not contacted with pot liquid, avoids electric leakage accident, and there have cooling water jecket outside tank body to be warm in tank to adjust It spends, every glass tube is all individually wound on glass tube with two rings for being fixed on floating drum upper and lower by two floating drums equipped with one in tank On cleaning brush composition floating sweeper, the porting of all floating drums and all use hose respectively into discharge outlet in reactor Be connected in parallel, intake and exhaust hose and into scupper hose draw reactor outside be connected to compressed air hose and water head tank, react The outer porting of device is connected to threeway one end, the another both ends of threeway in succession respectively an air inlet normally closed solenoid valve and an exhaust it is normal Solenoid valve is closed, is opened when intake solenoid valve is powered, compressed air enters each floating drum through intake and exhaust hose, by the hydraulic pressure in floating drum It is arranged out to the water head tank being higher by above reactor top is placed in, floating drum floats upwards at this time, drives cleaning brush on glass tube Attachment cleaned with water upwards, when floating drum float to top when intake solenoid valve power-off close, exhaust solenoid valve be powered open, Water in water head tank injects each floating drum, and the air in floating drum is slowly discharged through intake and exhaust hose to outside tank body, and floating drum is slow Sinking cleans downwards the attachment on glass tube with water with cleaning brush, and daily cleans with water after number is set with two micro computers Time control switch controls two solenoid valves in air inlet-outlet pipe threeway, realizes and automatically controls, and reactor upper side has one to go out Liquid mouth is connect with bottom liquid inlet mouth with pipeline, and a pipeline pump is housed between pipeline, the liquid pipeline pump that liquid outlet is come out It is pumped into reactor after pressurization, liquid is made to stir circulation up and down, acting on is to keep strain in reactor in liquid or algae equal It is even to receive illumination.

Share single floating drum form: similar, smaller for the specification and few tubulation reactor with more floating drum forms, clearly Brush is driven by floating drum, and operating procedure is identical, is only distinguished and is driven cleaning brush in multiple keg buoys and individually share Big floating drum drives cleaning brush, shares single floating drum form and requires strictly tubulation arrangement, it is necessary to uniformly arrange around concentric circles, The cleaning brush that can go sliding set on glass tube by the several shift forks for stretching to each glass tube being fixed on shared floating drum.

Detailed description of the invention:

Attached drawing 1 is the structure principle chart of more than the one floating drum culture photosynthetic bacteria of scheme；

Attached drawing 2 is to sink down into bottom schematic view after more than the one floating drum floating drum of scheme fills the water；

Attached drawing 3 is the structure principle chart of more than the one floating drum culture chlorella of scheme；

Attached drawing 4 is individual glass pipe and single group floating sweeper schematic diagram；

Attached drawing 5 is the structure principle chart that scheme two shares floating drum culture photosynthetic bacteria.

Below in conjunction with Figure of description, with embodiment, the invention will be further described:

The first, for cultivating photosynthetic bacteria, attached drawing 1 is explained；Reactor tank body 1 is made of resistant material, (the reactor tank body of this example use stainless steel manufacturing specification for), it is supported by four supporting legs 18, glass tube 2 is straight It stands through the bottom and top of reactor tank body 1, (this example uses 6Lucite tube) sealing shroud is used up and down 3 seal impenetrating mouth gaps, hang incandescent lamp bulb string 4 in 2 inner wall of glass tube, every glass tube 2 is peripherally equipped with by upper and lower two The floating sweeper of cleaning brush 13 and left and right two floating drums 17 composition, the porting of all floating drums 17 of six groups of floating sweepers With it is in parallel respectively into discharge outlet, total porting 11 in 1 middle part outside of reactor tank body is led to intake and exhaust hose 19, with into row Water hose 20 lead to the middle part of reactor tank body 1 outside always into discharge outlet 12, confirm bottom liquid valve door 15 and sample tap faucet After 16 close, culture medium and strain mixed liquor close material inlet valve 5 from top after material inlet valve 5 fills reactor tank body 1, follow Ring liquid outlet 7 is usedPvc pipe is connect with the inlet of circulating pump 24, culture solution through circulating pump 24 pressurization after, through bottom into Mouthful normally opened valve 14 enter to be formed in reactor tank body 1 from bottom and circulate strain is avoided to precipitate, cooling water is from collet Water inlet 9 enters cooling jacket 8, is discharged from collet water outlet 10 to adjust the temperature in reactor tank body 1,23 note of water head tank Full water, 4 electrified light emitting incubation of incandescent lamp bulb string start, and the normally closed power-off of air solenoid valve 21 is in closed state, and energization is opened Normally closed air solenoid valve 22 is opened, compressed air enters each floating drum 17 through total porting 11 and intake and exhaust hose 19 at this time, respectively Water in a floating drum 17 is extruded by compressed air, through pressing upper water head tank 23 into scupper hose 20 and always into discharge outlet 12, is covered Floating sweeper on glass tube 2 floats, and cleaning brush 13 upwards cleans glass tube 2 with water, when floating sweeper floats to top Normally closed air solenoid valve 22 and all power-off of normally closed air solenoid valve 21, compressed air be enclosed in the water of the inner head tank of floating drum 17 without Method injection, floating sweeper just float on above always, and the sweeper that such as to float sinks to bottom, only by normally closed air solenoid valve 21 It is powered and opens, the air in floating drum 17 is discharged, and the water in water head tank 23 is just slowly injected into each floating drum 17, floats and cleans Device, which slowly sinks, downwards again cleans glass tube 2 with water with cleaning brush 13, normally closed air solenoid valve 21 and normally closed air solenoid valve 22 are controlled by two micro computer time control switch, and floating sweeper floats, sinks to being spaced according to set time automatically to glass Pipe 2 is cleaned with water, entire incubation, and the pressure in reactor tank body 1 is automatically adjusted by the breather valve 6 of top, with the external world Pressure is consistent, and liquid is discharged from liquid valve 15 after cultivation cycle.

The second, attached drawing 3 is explained for cultivating chlorella；

Chlorella culture and photosynthetic bacteria culture presented hereinbefore are similar, and the essentially identical technique of process slightly has difference, It is participated in since photosynthetic bacteria can not need air with Anaerobic culturel, incubation, and chlorella incubation needs to absorb dioxy Change carbon, so incubation needs are constantly passed through air, the carbon dioxide in air is allowed to be dissolved in culture solution, it is extra in air The oxygen released after ingredient and chlorella reaction will be discharged in time, and technique, which is a difference in that, is exported to bottom in circulating pump 24 A Venturi nozzle 25 is sealed in pipeline between portion's normally opened valve 14, the air entry of Venturi nozzle 25 is by air filter 26 provide clean air, and continuously enter in reactor tank body 1, and extra gas is discharged from top breather valve 6, by It is different from the spectrum that photosynthetic bacteria is liked in the spectrum that chlorella is liked, so must be by the incandescent lamp string 4 in every glass tube 2 It is replaced with fluorescent lamp beam 27, other operating procedures are consistent with the step of cultivating photosynthetic bacteria, are not repeated herein.

Third, for cultivating photosynthetic bacteria, attached drawing 5 is explained；

Structure of reactor is similar, distinguishes at only one, is exactly the difference of more floating drums and shared monobuoy, shares floating drum Fixed two groups of shift forks 29, every shift fork up and down are connect with the cleaning brush 13 covered on glass tube 2 respectively on 28, by sharing floating drum 28 clean glass tube 2 with water to drive to clean with water, and the operating procedure of incubation is consistent with more floating drum fully reactings, here no longer It repeats.

The bright beneficial effect of the present invention has the following aspects:

One, efficiently utilizes electric light source, and the waste irradiation range of electric light source is only limitted to two ports of every glass tube, absolutely Most of light source is all radiated in the liquid in reactor tank.

Two, energy automatic cleaning in vapor tight tank body is attached to the attachment in glass tube walls, keeps electric light source not covered, right Culture solution remains intensity of illumination, to shorten cultivation cycle, manually cleans link with water due to removing from, reduces labor cost and section Province cleans the time with water, to improve the utilization rate of reactor.

Three, will not can be obtained high purity product, be able in the microorganism of vapor tight tank In vivo culture by other biological pollutions Improve added value of product.

For four, since the electric light source of different spectrum is easy to change, inflation cycle and sealing and circulating conversion are simple, adapt to difference The luminous energy microculture of type can accomplish a variety of luminous energy microorganisms of a set of bioreactor culture to save equipment investment.

Claims

1. the closed bioreactor of the shell and tube of automatic cleaning tube wall, technical characteristic is:

One, in reactor tank body upright several heavy calibers (More than) lucite tube or tempering of high transparency Glass tube, and run through tank base and top cover, the electric light such as incandescent lamp bulb or fluorescent lamp or LED light are hung in glass inside pipe wall Source, electric light source do not contact with pot liquid and avoid electric leakage accident, become the heavy caliber fluorescent glass of submergence in a liquid after energization Pipe.

Two, every glass pipe outer wall is furnished with one group by two rings around the annular cleaning brush of glass tube, and annular cleaning brush is by tank Floating drum driving, the air inlet-outlet pipe and intake-discharge pipe of floating drum connect with the compressed air hose in the tank body external world and water head tank respectively, Intake and exhaust are controlled with the closure or openness of valve, being blown into compressed air to floating drum, then floating drum floats, and it is empty to exhaust compression in floating drum Then water floating drum sinking is injected after gas, the annular cleaning brush being looped around on glass tube is as floating drum floats and sinks automatically to glass tube On attachment clean with water.