CN101402915A - High-efficiency photobioreactor for cultivation of photosynthetic bacteria - Google Patents

High-efficiency photobioreactor for cultivation of photosynthetic bacteria Download PDF

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Publication number
CN101402915A
CN101402915A CNA2008100414162A CN200810041416A CN101402915A CN 101402915 A CN101402915 A CN 101402915A CN A2008100414162 A CNA2008100414162 A CN A2008100414162A CN 200810041416 A CN200810041416 A CN 200810041416A CN 101402915 A CN101402915 A CN 101402915A
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Prior art keywords
water cycle
photosynthetic bacterium
light source
water circulation
brush
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Granted
Application number
CNA2008100414162A
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Chinese (zh)
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CN101402915B (en
Inventor
黄旭雄
庄林川
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Shanghai Maritime University
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Shanghai Maritime University
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/24Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00Means for providing, directing, scattering or concentrating light
    • C12M31/10Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED

Abstract

The invention discloses a high efficient photo-bioreactor for the culture of photosynthetic bacteria, which consists of a control system, a transparent cylindrical barrel body, an internal light source, an internal modulator tube sleeve brush, a water circulation flow guide device, a heating device and an electronic cooling device. The photo-bioreactor is characterized in that: the system is totally sealed; the internal light source and an external natural light source can be utilized simultaneously, the internal light source is arranged at the centre of the barrel body and protected by a quartziferous modulator tube sleeve, and the outer wall of which is provided with the lift type annular brush; the water circulation flow guide device is positioned at one side of the barrel body and comprises a mini submerged pump and a water circulation guide pipe; the lower end of the water circulation guide pipe is connected with a pump and the 1/3 upper part is provided with a column of water circulation outlets, direction of which is not vertical to the tangent plane of the barrel wall at the closer side, thus ensuring liquid in the reactor is fully stirred under anaerobic conditions; and the heating device and the cooling device are integrated in the water circulation guide pipe. The photo-bioreactor has convenient operation, low cost and safe production and can fulfill high efficient and continuous culture of photosynthetic bacteria.

Description

A kind of high-efficiency photobioreactor of cultivating photosynthetic bacterium
Affiliated technical field
The present invention relates to a kind of efficient, practical, bioreactor cheaply that is used to cultivate photosynthetic bacterium.
Background technology
Photosynthetic bacterium is that a class is under different physical environments, has multiple heterotrophism function (fixed nitrogen, denitrogenation, solid carbon, sulfide oxidation etc.), with the material cycle (nitrogen, phosphorus, sulphur) of occurring in nature substantial connection is arranged, in natural purification process, play an important role, can under anaerobic not put the general name of the photosynthetic gram negative bacterium of oxygen.At present, photosynthetic bacterium has been widely used in a plurality of fields such as aquatic products, agricultural, environmental protection, medicine.In aquaculture, photosynthetic bacterium can be used as the water quality cleansing agent of aquaculture water, additive, fish, shrimp, the somatomedin of the shellfish young and the purposes such as control of fish and shrimp diseases of Articial bait making.In the aquaculture process, probiotic bacteriums such as use photosynthetic bacterium are accepted by increasing raiser as the important production measure of green safety fishery products.
The training method of photosynthetic bacterium has lonely illumination cultivation of totally enclosed and open gas illumination cultivation two classes that decline at present.The lonely illumination cultivation of totally enclosed is more satisfactory photosynthetic bacterium training method, photosynthetic bacterium growth speed is fast under appropriate condition, the living contaminants degree is low, cultivate the cell density height that can reach, but carrying out culture condition has relatively high expectations, generally only limit to the laboratory and carry out cultivation on a small scale, can not satisfy the productivity needs with flask.The open gas illumination cultivation that declines generally adopts large-sized open mouthed container cultivations such as cement pit, and little inflation during cultivation is slowly stirred the photosynthetic bacterium nutrient solution in the container.The open gas illumination cultivation required equipment that declines is fairly simple, can be used for the productivity large scale culturing, but the living contaminants degree is big in the culturing process, poor growth, and it is low to cultivate the cell density that can reach, and cultivates failure easily.
Bioreactor is a kind of typical closed culture apparatus.Since the 1980's, the researchdevelopment of external bioreactor is rapid, is mainly used in the cultivation of unicellular algae, has now developed the bioreactor of various ways such as duct type, flat, pot type.The principal feature of various bioreactors is: maximum surface area and volume ratio, the most effective light-source system, luminous energy are delivered to the shortest light path of little algae, the most effective mixed cycle etc.Utilize bioreactor can carry out high-density, high yield and high-efficient culture unicellular algae.But the manufacturing cost of conventional bioreactor is high, and the envrionment conditions of using is required harshness, has limited its application in aquaculture production.In addition, because the culturing process of unicellular algae is the process of an aerobic product oxygen, and the optimal culture condition of photosynthetic bacterium is the anaerobic environment, therefore directly the conventional bioreactor of use is cultivated photosynthetic bacterium and is not suitable for, show as the production cost height, the attached wall of bacterium is serious, influences the luminous energy transmission, and the growth efficiency of photosynthetic bacterium is difficult to reach re-set target.
Summary of the invention
Technical problem to be solved by this invention is that the photosynthetic bacterium at increasingly extensive application in the aquaculture provides a kind of easy to operate, with low cost, high-efficiency photobioreactor safe in utilization, use this bioreactor to cultivate photosynthetic bacterium, the optimum temperuture and the competent illumination condition of photosynthetic bacterium growth can be provided, and have and effectively prevent the pollution of extraneous assorted bacterium and harmful animal photosynthetic bacterium growth, have easy to operate, with low cost, safe in utilization, can realize the round-the-clock high-density cultured continuously of photosynthetic bacterium.
The technical solution adopted for the present invention to solve the technical problems is: a kind of high-efficiency photobioreactor of cultivating photosynthetic bacterium, comprise Controlling System, transparent cylindrical staving (magnetic force brush is installed), built-in light source, Built-in light pipe box brush, water cycle guiding device, heating and electronic refrigeration plant, fluid inlet and liquid outlet.Described reactor is totally-enclosed; described built-in light source is positioned at the staving center of circle; protect by the clear lamp pipe box; the light tube jacket outer wall is equipped with the pull-type brush; described water cycle guiding device is partial to staving one side, is made up of the water cycle conduit of miniature immersible pump, both ends open, and water cycle conduit lower ending opening connects miniature immersible pump; a file posticum is established with the upper part in conduit lower end 1/3, and described heating and electronic refrigeration plant are incorporated in the water cycle conduit.
Described a kind of high-efficiency photobioreactor of cultivating photosynthetic bacterium is characterized in that built-in light source is plant fluorescent tube or fluorescent tube bulb.
Described a kind of high-efficiency photobioreactor of cultivating photosynthetic bacterium is characterized in that the light tube jacket upper end is installed in circular bung center, and is detached type.
Described a kind of high-efficiency photobioreactor of cultivating photosynthetic bacterium, the brush that it is characterized in that the pull-type brush is an annular, around light tube jacket, its material is the stainless steel steel wire ring of sponge wrapping, the pull bar of hairbrush is a stainless steel steel wire bar, the aperture on the bung is passed in the upper end, and the pull-type hairbrush can slide up and down along light tube jacket under the effect of external force.
Described a kind of high-efficiency photobioreactor of cultivating photosynthetic bacterium is characterized in that water cycle conduit upper end is installed on the bung, and is detached type that the tangent plane of the direction of the posticum of water cycle conduit and reactor round tank wall is 15 °~75 ° angles.
Described a kind of high-efficiency photobioreactor of cultivating photosynthetic bacterium, it is characterized in that water cycle conduit upper end open is on bung, the supply lead of heating rod, refrigerator pipes, temperature probe and the miniature immersible pump opening by water cycle conduit upper end respectively enters and is fixed in water cycle pipe inner wall four sides, and the opening double as fluid inlet of water cycle conduit upper end also seals with cork.
Compared with prior art, advantage of the present invention is: the one, and the bioreactor of totally enclosed photosynthetic bacterium has effectively prevented the pollution to the cultivation photosynthetic bacterium of extraneous assorted bacterium and harmful animal, can realize the highly purified cultivation of photosynthetic bacterium; The 2nd, built-in light source can utilize natural light and built-in light source simultaneously in conjunction with transparent staving, and the maximum long-pending and volume ratio of optical surface is provided in the design, makes the light source utilization ratio reach more high-level efficiency, has ensured the sufficient light source of photosynthetic bacterium high-density growth; The 3rd, by the magnetic force brush on the bucket wall, installed with on light tube jacket the light tube jacket brush is installed, can effectively prevent to ensure that because of the photosynthetic bacterium problem that transmittance that the attached wall of light causes reduces that becomes luminous energy can effectively penetrate nutrient solution; The 4th, the water circle device of installing structurally helps the abundant stirring that inside reactor is cultivated liquid, liquid during device work in the reactor is under the acting in conjunction of micro pump and current and bucket wall, existing circulation is up and down stirred, also there is horizontal cyclic to stir, so not only avoid photosynthetic bacterium to sink to the bottom effectively, and can ensure that all photosynthetic bacteriums fully accept illumination and grow, use water circle device of the present invention simultaneously and guaranteed the anaerobic condition of nutrient solution in the whipping process to greatest extent; The 5th, temperature control system can be controlled the temperature of photosynthetic bacterium nutrient solution exactly, makes it to remain in the optimum temperature range of photosynthetic bacterium growth; The 6th, total system is easy to install and use, and is with low cost, can realize efficient a large amount of cultivation of photosynthetic bacterium, is adapted at aquafarm and uses.
Description of drawings
Fig. 1 is an one-piece construction synoptic diagram of the present invention.
Fig. 2 is a bung part vertical view of the present invention.
Fig. 3 is the bucket of the present invention structural map at the end.
Fig. 4 is bung of the present invention and the side perspective view that is installed in annex on the bung.
In the accompanying drawing, 1. cylindrical staving, 2. circular bung, 3. semisphere stainless steel base, 4. liquid outlet, 5. valve, 6. A-frame, 7. magnetic force brush, 8. plant fluorescent tube, 9. light tube jacket, 10. lamp cover brush, 11. stainless steel steel wires, 12. miniature immersible pump, 13. water cycle conduits, 14. recycle the water hole, 15. the temperature sense probe, 16. heating rods, 17. refrigerator pipes, 18. electronic refrigerator, 19. silica gel plugs, 20. control enclosurees.
Embodiment
Describe embodiments of the invention in detail below in conjunction with accompanying drawing.
Embodiment 1:
A kind of high-efficiency photobioreactor of cultivating photosynthetic bacterium, comprise 30 centimetres of the diameters that transparent acrylic makes, high 120 centimetres cylindrical staving (1), there is the acrylic bung (2) of a circle staving upper end, by 4 bayonet sockets (21) can with staving (1) tight closure, the staving lower end is a semisphere stainless steel base (3), the liquid outlet (4) of a 2cm diameter is opened at the base center, liquid outlet is connecting valve (5), the bucket back welding connects A-frame (6), on the bucket wall magnetic force brush (7) is installed, built-in light source is one long 100 centimetres a plant fluorescent tube (8), the light tube jacket (9) of a quartziferous bottom end seal of fluorescent tube outside cover, light tube jacket (9) upper end is fixed on bung (2) center by threaded connector, an annular lamp pipe box brush (10) is installed in light tube jacket (9) outside, light tube jacket brush (10) is connected on the stainless steel steel wire (11), push-and-pull stainless steel steel wire (11) can make light tube jacket brush (10) upwards slide along light tube jacket (9) downwards, the water cycle guiding device mainly is made up of the water cycle conduit (13) of a miniature immersible pump (12) and both ends open, the water outlet of submersible pump (12) and water cycle conduit (13) lower ending opening join, water cycle conduit (13) upper end is fixed on bung (2) one sides by threaded connector and is opened on outside the bung (2), a file posticum (14) is established with the upper part in water cycle conduit (13) lower end 1/3, a temperature sense probe (15) is installed at water cycle conduit (13) position that links to each other with miniature immersible pump (12), inner chamber in water cycle conduit (13), a 100W heating rod (16) is installed in 1/3 scope of lower end, a refrigerator pipes (17) is installed on top, refrigerator pipes (17) links to each other with electronic refrigerator (18), the opening double as fluid inlet of water cycle conduit (13) on bung (2), fluid inlet seals with the silica gel plug (19) of a plug-in, bung (2) is gone up a control enclosure (20) is installed, built-in light source is installed on the control enclosure, refrigeration, heating, the trip switch of device such as water cycle and connection lead, control enclosure is connected by the plug-in joint with each work original paper, behind default working parameter, can realize switch lamp, automatically heat or refrigeration, functions such as automated cycle.
Implementation result:
2008 in the Shanghai Ocean University laboratory and Weishan County, the Shandong plant photosynthetic bacterium of using this reactor, the closed lonely illumination cultivation of conventional Nylon Bag and the little gas illumination cultivation of open glass flume to carry out 6 batches respectively cultivate, at identical culture temperature (30 ℃ ± 1 ℃), under identical inoculum density (1: 20) and the identical culture medium condition, use this reactor through 5 days cultivation, the concentration of photosynthetic bacterium can reach 8.7*10 10Cell/mL can the amount of gathering be 60L at every turn; The closed lonely illumination cultivation of traditional Nylon Bag was cultivated through 7 days, and the concentration of photosynthetic bacterium can reach 4.2*10 10Cell/mL can the amount of gathering be 10L at every turn.Traditional opening declines the gas illumination cultivation through 15 days cultivation, and the concentration of photosynthetic bacterium can reach 7.5*10 9Cell/mL can the amount of gathering be 50L at every turn.Compare with traditional photosynthetic bacterium culture apparatus, the growth population of unit of water body photosynthetic bacterium is the comparison index with the unit time, the growth efficiency of device cultivation photosynthetic bacterium of the present invention is 2.9 times of the closed illumination cultivation of conventional nylon bag, is 34.8 times of the little gas illumination cultivation of traditional open tank.

Claims (6)

1, a kind of high-efficiency photobioreactor of cultivating photosynthetic bacterium comprises Controlling System, transparent cylindrical staving, built-in light source, Built-in light pipe box brush, water cycle guiding device, heating and electronic refrigeration plant, fluid inlet and liquid outlet.It is characterized in that described reactor is totally-enclosed; described built-in light source is positioned at the staving center of circle; protect by the clear lamp pipe box; the light tube jacket outer wall is equipped with the pull-type brush; described water cycle guiding device is partial to staving one side, is made up of the water cycle conduit of miniature immersible pump, both ends open, and the outlet of water cycle conduit lower end connects miniature immersible pump; 1/3 establishes row with the upper part recycles the water hole, and described heating and refrigeration plant are incorporated in the water cycle conduit.
2, a kind of according to claim 1 high-efficiency photobioreactor of cultivating photosynthetic bacterium is characterized in that described built-in light source is plant fluorescent tube or fluorescent tube bulb.
3, a kind of according to claim 1 high-efficiency photobioreactor of cultivating photosynthetic bacterium is characterized in that described light tube jacket upper end is installed in circular bung center, and is detached type.
4, a kind of according to claim 1 high-efficiency photobioreactor of cultivating photosynthetic bacterium, the brush that it is characterized in that described pull-type brush is an annular, around light tube jacket, its material is the stainless steel steel wire ring of sponge wrapping, the pull bar of hairbrush is a stainless steel steel wire bar, the aperture on the bung is passed in the upper end, and the pull-type hairbrush can slide up and down along light tube jacket under the effect of external force.
5, a kind of according to claim 1 high-efficiency photobioreactor of cultivating photosynthetic bacterium, it is characterized in that described water cycle conduit upper end is installed on the bung, and be detached type, the tangent plane of the direction of the posticum of water cycle conduit and reactor round tank wall is 15 °~75 ° angles.
6, a kind of according to claim 1 high-efficiency photobioreactor of cultivating photosynthetic bacterium, it is characterized in that described water cycle conduit upper end open is on bung, the supply lead of heating rod, refrigerator pipes, temperature probe and the miniature immersible pump opening by water cycle conduit upper end respectively enters and is fixed in water cycle pipe inner wall four sides, and the opening double as fluid inlet of water cycle conduit upper end also seals with cork.
CN2008100414162A 2008-08-05 2008-08-05 High-efficiency photobioreactor for cultivation of photosynthetic bacteria Expired - Fee Related CN101402915B (en)

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Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2469198A (en) * 2009-04-04 2010-10-06 Robert Terrence Perry Bioreactor having interior illumination, removable reflector and fluid circulation system
CN101974412A (en) * 2010-09-30 2011-02-16 广州大学 Photosynthetic hydrogen preparation device
CN102973961A (en) * 2012-11-22 2013-03-20 华侨大学 Ozone sterilization method for photobioreactor for culturing photosynthetic bacteria in large scale
CN102994424A (en) * 2012-11-22 2013-03-27 华侨大学 Method for efficiently and continuously collecting super-concentrated photosynthetic bacteria thalli
CN103122306A (en) * 2011-11-21 2013-05-29 镇江日泰生物工程设备有限公司 Photosynthetic cultivation fermentation device
CN105969661A (en) * 2016-07-08 2016-09-28 中国科学院上海高等研究院 Column type and pyramid-tube type coupled photoproduction reactor, application and culture method
CN106148183A (en) * 2016-07-08 2016-11-23 中国科学院上海高等研究院 There is photoproduction reaction device, application and the cultural method of bionical fractal tree
CN108220150A (en) * 2018-03-09 2018-06-29 岳晗禹 A kind of Bacteria Culture experimental provision
CN110240998A (en) * 2018-03-07 2019-09-17 曾坚 The closed bioreactor of the shell and tube of automatic cleaning tube wall
CN112063519A (en) * 2020-09-14 2020-12-11 中国科学院重庆绿色智能技术研究院 Photosynthetic bacteria continuous culture device and control method thereof
CN116875451A (en) * 2023-09-07 2023-10-13 青岛中仁澳兰生物工程有限公司 Efficient bacterial fermentation culture device
RU224755U1 (en) * 2024-01-26 2024-04-02 Федеральное государственное бюджетное образовательное учреждение высшего образования "Дальневосточный государственный технический рыбохозяйственный университет" (ФГБОУ ВО "Дальрыбвтуз") Photobioreactor for oxygen generation based on microalgae Chlorella vulgaris

Family Cites Families (3)

* Cited by examiner, † Cited by third party
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CN2199988Y (en) * 1994-07-16 1995-06-07 北京威格尔国际合作发展公司 Photosynthetic bacteria liquid culture apparatus
WO2001039591A2 (en) * 1999-11-30 2001-06-07 Brassica Protection Products, L.L.C. Airlift bioreactor for harvesting sprouts
CN2918431Y (en) * 2006-06-12 2007-07-04 河南农业大学 Photosynthetic microorganism hydrogen production reactor

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2469198A (en) * 2009-04-04 2010-10-06 Robert Terrence Perry Bioreactor having interior illumination, removable reflector and fluid circulation system
CN101974412A (en) * 2010-09-30 2011-02-16 广州大学 Photosynthetic hydrogen preparation device
CN101974412B (en) * 2010-09-30 2013-03-13 广州大学 Photosynthetic hydrogen preparation device
CN103122306A (en) * 2011-11-21 2013-05-29 镇江日泰生物工程设备有限公司 Photosynthetic cultivation fermentation device
CN102973961A (en) * 2012-11-22 2013-03-20 华侨大学 Ozone sterilization method for photobioreactor for culturing photosynthetic bacteria in large scale
CN102994424A (en) * 2012-11-22 2013-03-27 华侨大学 Method for efficiently and continuously collecting super-concentrated photosynthetic bacteria thalli
CN102994424B (en) * 2012-11-22 2015-08-05 华侨大学 A kind of high-efficiency and continuous collects the method for hyperconcentration photosynthetic bacterial thallus
CN106148183A (en) * 2016-07-08 2016-11-23 中国科学院上海高等研究院 There is photoproduction reaction device, application and the cultural method of bionical fractal tree
CN105969661A (en) * 2016-07-08 2016-09-28 中国科学院上海高等研究院 Column type and pyramid-tube type coupled photoproduction reactor, application and culture method
CN105969661B (en) * 2016-07-08 2018-05-15 中国科学院上海高等研究院 Pillar coupling pyramid tubular type photoproduction reaction device, application and cultural method
CN106148183B (en) * 2016-07-08 2018-06-19 中国科学院上海高等研究院 Photoproduction reaction device, application and cultural method with bionical Fractal Tree shape structure
CN110240998A (en) * 2018-03-07 2019-09-17 曾坚 The closed bioreactor of the shell and tube of automatic cleaning tube wall
CN108220150A (en) * 2018-03-09 2018-06-29 岳晗禹 A kind of Bacteria Culture experimental provision
CN112063519A (en) * 2020-09-14 2020-12-11 中国科学院重庆绿色智能技术研究院 Photosynthetic bacteria continuous culture device and control method thereof
CN112063519B (en) * 2020-09-14 2022-06-10 中国科学院重庆绿色智能技术研究院 Photosynthetic bacteria continuous culture device and control method thereof
CN116875451A (en) * 2023-09-07 2023-10-13 青岛中仁澳兰生物工程有限公司 Efficient bacterial fermentation culture device
CN116875451B (en) * 2023-09-07 2023-12-01 青岛中仁澳兰生物工程有限公司 Efficient bacterial fermentation culture device
RU224755U1 (en) * 2024-01-26 2024-04-02 Федеральное государственное бюджетное образовательное учреждение высшего образования "Дальневосточный государственный технический рыбохозяйственный университет" (ФГБОУ ВО "Дальрыбвтуз") Photobioreactor for oxygen generation based on microalgae Chlorella vulgaris

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