CN110240439A - A kind of preparation method of the luminous ecological substrate of the cured high-strength light of microorganism - Google Patents

A kind of preparation method of the luminous ecological substrate of the cured high-strength light of microorganism Download PDF

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CN110240439A
CN110240439A CN201910575112.2A CN201910575112A CN110240439A CN 110240439 A CN110240439 A CN 110240439A CN 201910575112 A CN201910575112 A CN 201910575112A CN 110240439 A CN110240439 A CN 110240439A
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preparation
substrate
microorganism
bacterium solution
raw material
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CN110240439B (en
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马强
舒杭
肖衡林
黄朝纲
刘昭
付华飞
向俊宸
胡斌
陈智
万娟
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Hubei University of Technology
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Hubei University of Technology
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    • C—CHEMISTRY; METALLURGY
    • C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
    • C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
    • C04B28/00—Compositions of mortars, concrete or artificial stone, containing inorganic binders or the reaction product of an inorganic and an organic binder, e.g. polycarboxylate cements
    • C—CHEMISTRY; METALLURGY
    • C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
    • C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
    • C04B41/00—After-treatment of mortars, concrete, artificial stone or ceramics; Treatment of natural stone
    • C04B41/009—After-treatment of mortars, concrete, artificial stone or ceramics; Treatment of natural stone characterised by the material treated
    • C—CHEMISTRY; METALLURGY
    • C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
    • C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
    • C04B41/00—After-treatment of mortars, concrete, artificial stone or ceramics; Treatment of natural stone
    • C04B41/45—Coating or impregnating, e.g. injection in masonry, partial coating of green or fired ceramics, organic coating compositions for adhering together two concrete elements
    • C04B41/50—Coating or impregnating, e.g. injection in masonry, partial coating of green or fired ceramics, organic coating compositions for adhering together two concrete elements with inorganic materials
    • C04B41/5007—Coating or impregnating, e.g. injection in masonry, partial coating of green or fired ceramics, organic coating compositions for adhering together two concrete elements with inorganic materials with salts or salty compositions, e.g. for salt glazing
    • C04B41/5014—Coating or impregnating, e.g. injection in masonry, partial coating of green or fired ceramics, organic coating compositions for adhering together two concrete elements with inorganic materials with salts or salty compositions, e.g. for salt glazing containing sulfur in the anion, e.g. sulfides
    • C—CHEMISTRY; METALLURGY
    • C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
    • C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
    • C04B41/00—After-treatment of mortars, concrete, artificial stone or ceramics; Treatment of natural stone
    • C04B41/60—After-treatment of mortars, concrete, artificial stone or ceramics; Treatment of natural stone of only artificial stone
    • C04B41/61—Coating or impregnation
    • C04B41/65—Coating or impregnation with inorganic materials
    • C—CHEMISTRY; METALLURGY
    • C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
    • C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
    • C04B2111/00—Mortars, concrete or artificial stone or mixtures to prepare them, characterised by specific function, property or use
    • C04B2111/00017—Aspects relating to the protection of the environment
    • C—CHEMISTRY; METALLURGY
    • C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
    • C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
    • C04B2111/00—Mortars, concrete or artificial stone or mixtures to prepare them, characterised by specific function, property or use
    • C04B2111/00474—Uses not provided for elsewhere in C04B2111/00
    • C04B2111/00732—Uses not provided for elsewhere in C04B2111/00 for soil stabilisation
    • C—CHEMISTRY; METALLURGY
    • C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
    • C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
    • C04B2111/00—Mortars, concrete or artificial stone or mixtures to prepare them, characterised by specific function, property or use
    • C04B2111/00474—Uses not provided for elsewhere in C04B2111/00
    • C04B2111/00767—Uses not provided for elsewhere in C04B2111/00 for waste stabilisation purposes
    • C—CHEMISTRY; METALLURGY
    • C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
    • C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
    • C04B2111/00—Mortars, concrete or artificial stone or mixtures to prepare them, characterised by specific function, property or use
    • C04B2111/80—Optical properties, e.g. transparency or reflexibility
    • C04B2111/807—Luminescent or fluorescent materials
    • C—CHEMISTRY; METALLURGY
    • C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
    • C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
    • C04B2201/00—Mortars, concrete or artificial stone characterised by specific physical values
    • C04B2201/50—Mortars, concrete or artificial stone characterised by specific physical values for the mechanical strength

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Ceramic Engineering (AREA)
  • Materials Engineering (AREA)
  • Structural Engineering (AREA)
  • Organic Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention belongs to the ecological slope protection fields of civil engineering, a kind of preparation method of luminous ecological substrate of the cured high-strength light of microorganism is specifically disclosed, which is prepared from the following materials: polypropylene fibre, high impact polystyrene, acrylic resin, bacterium solution, consolidating fluid, earth complex luminescent component, raw material soil and water.The present invention is added high impact polystyrene, acrylic resin, increases substrate intensity by the way that polypropylene fibre is added in the soil body to soil body reinforcement.Substrate is formed after microorganism solidification, substrate weight is light, intensity is high, the pollution-free and feature that can shine to assign, and can reduce the slope surface load of side slope, increases slope stability.Substrate of the invention meets protecting slope ecology requirement, can be widely used in side slope protection and landscape design, high-strength light and landscape effect is significant, is that a kind of application range is wide, ecological substrate of strong applicability.

Description

A kind of preparation method of the luminous ecological substrate of the cured high-strength light of microorganism
Technical field
The present invention relates to the technical field of ecological slope protection of civil engineering, and in particular to a kind of cured high-strength light of microorganism The preparation method of luminous ecology substrate.
Background technique
Ecological slope protection and slope landscape engineering are for the purpose of engineering construction and environmental protection, using substrate to the anchor of ground Gu effect, carries out slope surface layer and superficial layer is stablized, slope surface disaster, soil erosion are prevented, is made to reach and extend construction project With the service life, the recovery of engineering wound is carried out rapidly, improves ecology, a practicable engineering technology of purpose of beautifying the environment, Have become the important engineering project that China's traffic of mountain area, urban construction and Real Estate Development landscape are made.China's ecological vestige Huge advance is achieved, has effectively contained soil erosion, ecological degeneration as caused by engineering construction and slope instability problem.But it is raw State substrate often not can guarantee higher intensity requirement during lighting, above have certain limitations in application, and in slope surface Before plant growth, the Scene construction of side slope is difficult, and furthermore ecological protection measure does not have instruction guide function.
It is poor and right in engineering material production engineering that traditional bank substrate consumes a large amount of resource, heavy workload, durability Environmental pollution is serious, is unable to planting plants and flowers and plants, it is difficult to afforest, finally largely destroy the ecological ring of original harmony Border.The solid luminous substrate of microorganism is mainly consolidated soil by the chemical effect of microbial reaction, prevented erosion, and is given birth to meeting When state environment needs, scape can also be made, meets the multifunctionality of slope protection substrate, the ambient enviroment where further betterment works Micro climate, the ecological environment of region where repairing and improveing engineering.
Summary of the invention
For the deficiencies in the prior art, the base the present inventors considered that lightweight after solidifying with microorganism shines Material can significantly improve its strength characteristics and physical characteristic, act in terms of slope landscape constructs and is oriented to instruction significantly, using model It will be very wide for enclosing.
The main object of the present invention is to increase the intensity of substrate and make it have the characteristics of luminescence, meets slope reinforcement need It asks, side slope protection and Scene construction, and then the preparation method that a kind of cured high-strength light of microorganism shines ecological substrate is provided. This method is added high impact polystyrene, acrylic resin, increases by the way that polypropylene fibre is added in the soil body to soil body reinforcement Substrate intensity.Substrate is formed after microorganism solidification, substrate weight is light, intensity is high, the pollution-free and spy that can shine to assign Point can reduce the slope surface load of side slope, increase slope stability.
To achieve the goals above, the technical solution adopted by the present invention is that:
A kind of preparation method of the luminous ecological substrate of the cured high-strength light of microorganism, the specific steps are as follows:
(1) preparation solidification microbial inoculum 1 and luminescent microorganism bacterium solution 2；
The solidification microorganism is Pasteur's sporosarcina (Sporosarcina sp.) and/or Brevibacillus laterosporus (Brevibacillus laterosorus)；
The luminescent microorganism be Photobacterium (Photobacterium), genus Shewanella (Shewanella) and/ Or Photorhabdus (Photorhabdus)；
Further, the solidification microorganism is Pasteur's sporosarcina；
Further, the luminescent microorganism is abalone luminous bacillus and/or photobacterium phosphoreum；
Further, the culture medium for solidifying microorganism is NH4+- YE culture medium；The culture medium of the luminescent microorganism is 2216E fluid nutrient medium；
The bacterium solution 1 the preparation method comprises the following steps: by the solidification microbial inoculant to NH4+When cultivating one section in-YE culture medium Between after collect thallus, obtain bacterium solution 1, the OD600 value of bacterium solution 1 is 1.80-2.20；
The bacterium solution 2 the preparation method comprises the following steps: the luminescent microorganism is seeded in 2216E fluid nutrient medium, culture one Bacterium solution is collected after the section time, then is centrifuged abandoning supernatant, collects thallus, thallus redissolution is taken (to contain in 0.01mol/L pH=7.0PBS 10mmol/L EDTA, 1mmol/L DTT (dithiothreitol (DTT))), it redissolves than being 2.5g thallus/(20-40) ml PBS, obtains bacterium solution 2；
(2) consolidating fluid is prepared；
Further, the consolidating fluid is by peptone, beef extract, glycerol, NaHCO3、MgSO4, urea and Ca (CH3COO)2With Water composition.
Further, each component content in the consolidating fluid are as follows: peptone 2.0g/L, beef extract 4.0g/L, glycerol 4.0g/L, NaHCO32.0g/L、MgSO45.0g/L, urea 30.0g/L and Ca (CH3COO)2 55.0g/L。
(3) after taking raw material soil to set baking oven drying, 1mm sieve is crossed, it is spare；
Further, the raw material soil is riverway sludge matter soil.
(4) toward sequentially adding polypropylene fibre, high impact polystyrene, acrylic acid tree in the sieving raw material soil of step (3) Rouge；
(5) water is added into mixture obtained by step (4), is then stirred evenly in blender；
(6) earth complex luminescent component is added into mixture obtained by step (5)；
Further, the earth complex luminescent component are as follows: firefly luciferase, D- luciferin, ethylenediamine tetra-acetic acid (EDTA) and Dithiothreitol (DTT) (DTT)；
Firefly luciferase: D- luciferin: EDTA:DTT weight ratio is 1:(1-2): (3-4): (1-2)；
(7) successively bacterium solution 1 obtained by trickle irrigation step (1) and bacterium solution 2 into sample obtained by step (6), are then immersed in cementing Consolidating fluid is sprayed in liquid or into sample, after conserving 28d, obtains the luminous ecological substrate of the cured high-strength light of microorganism；
In the practical operation of building site, by inject bacterium solution after sample mixed it is uniform after be sprayed in side slope, then to sample Consolidating fluid is sprayed, by repeatedly spraying, forms high-strength light luminescent substrate after sufficiently combining.
The dosage of bacterium solution 1: raw material soil weight=1mL:(1-6 in step (3) after drying, sieving) g；
The dosage of bacterium solution 2: raw material soil weight=1mL:(3-8 in step (3) after drying, sieving) g；
Further, the dosage of bacterium solution 1: raw material soil weight=1mL:(2.5-3.5 in step (3) after drying, sieving) g；
The dosage of bacterium solution 2: raw material soil weight=1mL:(4.5-5.5 in step (3) after drying, sieving) g；
Further, stirring rate is set as 110-125r/min in the step (5), and mixing time is 10~20min.
Further, the polypropylene fibre uses 9mm grades.
Further, the high impact polystyrene particle uses diameter 2-3mm grades.
Further, the acrylic resin particle uses diameter 1-2mm grades.
Further, the substrate each component volume is (to account for the quality percentage of drying in step (3), the raw material soil after sieving Number):
Polypropylene fibre (0.2%~1%), high impact polystyrene (1%~5%), acrylic resin (1%~3%), Water (50-70%), earth complex luminescent component (0.2%~0.4%).
Since ATP is present in all living cells including microorganism, in firefly luciferase FL and Mg2+Work Under, D- luciferin LH2Adenylylation occurs with ATP and is activated, the D- luciferin and firefly luciferin after activation Enzyme combines, and forms D- luciferin-AMP complex, and release pyrophosphoric acid (PPi).The complex is by molecular oxygen oxidation, shape At excited state complex P*- EAMP releases CO2, emit light when the compound returns to ground state from excitation state.Its reaction equation are as follows:
FL·Ln-AMP+O2→[P*-FL·AMP]+CO2
[P*-FL·AMP]→FL-P+hv+AMP
Polypropylene fibre has many advantages, such as that intensity is high, elasticity is good, wear-resisting, corrosion-resistant, and self property stabilization will not be with Microbial reaction and decompose, and by generate precipitation of calcium carbonate it is cemented together, interfacial force can be formed, can effectively be inhibited Crack extension increases solidification intensity and improves the toughness and globality of lightweight substrate, prevents the soil body from destroying suddenly, solid for microorganism Change provides attachment condition, improves solidification effect.
High impact polystyrene (HIPS) is the thermoplastic material made of elastomer-modified polystyrene, has and easily adds Work has rigidity, dimensionally stable and transparent characteristic, plays the role of toughening blast.The material itself is transparent, and microorganism issues Light can pass through material and emit, have a very big promotion to the brightness inside substrate, also solve itself after microorganism solidification The limitation of low impact strength.
Acrylic resin has good guarantor's light colour retention, water-fast corrosion resistance, fast drying, is easy to construct, and has excellent Richness, gloss, hardness, solvent resistance, weatherability, not only improve substrate overall brightness, while also increasing substrate Intensity.
Ethylenediamine tetra-acetic acid (EDTA) and dithiothreitol (DTT) (DTT) can protect the active group of enzyme active center, prevent The sulfydryl of firefly luciferase is oxidized.
Since infiltration coefficient and water absorption rate and cycle grouting are presented negative correlativing relation, cycle grouting reaches three times or more When, solidification effect variation tendency gradually tends to be steady, and in addition the hole of lightweight substrate is more, also provides for the solidification of microorganism Good reacting environment, the precipitation of calcium carbonate generated can be secured firmly in substrate pores, and keep internal material all cementing Together, it is obviously improved the intensity of lightweight substrate, the substrate intensity after solidification much meets ecology Gu Po requirement.
The technical scheme is that special microbial reaction being fixed on selected carrier, making its high aggregation and being protected Bioactivity is held, the technology that can quickly, be largely proliferated under optimum conditions.The advantages of this technology is the bacterium itself used Be it is nontoxic and pollution-free, environment will not be impacted, do not have toxic gas discharge during the reaction, and probe intensity is special Property and physical characteristics are good, and luminescent properties are superior.By urease-producing microorganism catalysis hydrolysis of urea, in the feelings for having calcium source Calcium carbonate crystal is generated under condition, and part calcium carbonate product provides medium for photobacteria, can launch after after chemical reaction Light, the luminescent substrate can be effectively carried out ecological slope protection, it can caused by being effectively prevented landslide, reduction soil erosion etc. Loss, and for landscape angle, ecological slope protection can also afforest and beautify exposed slope surface, thus more ecoscape Effect.Luminescent substrate is applied into slope project, can be used as sight spot decoration, various landscape patterns is constructed and is used as guide mark Know.Substrate can be carried out in conjunction with side slope own characteristic, designed multi-level luminescent designs in slope surface, be combined with each other with retaining wall, phase It mutually influences, reaches landscaping effect, ecocatas-trophe can also be reduced, protection environment is convenient for driver and conductor to eliminate fatigue, improves and appreciate Degree.Substrate can also be designed as to artistic written form or pictorial form, show cultural characteristics.To sum up, in slope treatment In landscape design, project cost can be reduced again by not only improving security protection, and can generate ecoscape, the places of cultural interest and landscape The comprehensive benefit of aesthstic three can finally embody its social benefit.
By substrate applications in geotechnical engineering slope protection field, substrate cracking resistance, anti-permeability performance and machinery can be significantly improved Intensity.Luminescent substrate has wide practical use in fields such as side slope protection, landscape designs, adaptable strong, widely applicable The features such as, the requirement of characteristic, environmental protection and aesthetics design is embodied, current environmentally protective, man and nature and harmonious development are met Theory.
Compared with prior art, the advantages and beneficial effects of the present invention are:
The advantage of the invention is that the multifunction of the composite base material, has the characteristics that strong applicability, use scope are wide.This The high-strength light substrate applications of invention can play the role of preferably reinforcing and going along with sb. to guard him, can adequately protect soil when side slope protection Body is firm, convenient for greening, is good for the environment, while substrate itself shines can be used as functional form ornament materials, in the side of beautifying the environment Face has positive effect.
Detailed description of the invention
Fig. 1 is the excitation spectrum of substrate made from embodiment 1.
Specific embodiment
Technical solution of the present invention is described in further detail below in conjunction with specific embodiments.It should be understood that institute Purpose for embodiment is the content that the present invention is further explained, and cannot be construed to protect the present invention in any sense The limitation of range.
In following embodiment, (model indicates 9mm grades of PPF-9-S-400/10 polypropylene fibres are as follows: length 9mm, tension Intensity is greater than the polypropylene fiber filaments fiber of 400MPa, section elongation percentage greater than 10%) it is purchased from auspicious prosperous cellulose plant；Diameter 2-3mm grades of PH-879 high impact polystyrenes are plasticized Co., Ltd purchased from outstanding achievement；1-2mm grades of DEGALAN P24N acrylic acid of diameter Resin is purchased from Guangzhou Bo Feng Chemical Industry Science Co., Ltd；11859 Pasteur's sporosarcina of ATCC, 27561 abalone of ATCC shine Bacillus, 11040 photobacterium phosphoreum of ATCC are purchased from Shanghai Lian Mai bioengineering Co., Ltd；Firefly luciferase (FL, 13.75mg/mL), D- luciferin (Ln, 264364) is purchased from Promega company；2216E fluid nutrient medium is purchased from Qing Daohai Rich Bioisystech Co., Ltd；EDTA, dithiothreitol (DTT) (DTT) are purchased from Sigma company；Other are conventional reagent and instrument.
Embodiment 1:
A kind of the step of luminous ecological substrate of the cured high-strength light of microorganism, preparation method, is as follows:
(1) it prepares bacterium solution: Pasteur's sporosarcina is seeded to NH4+(culture medium is extracted containing yeast on-YE culture medium Object 20.0g/L, (NH4)2SO410.0g/L adjusts pH value to 9) with 2g/LNaOH solution, in 25 DEG C, 140r/min shaken cultivation Thallus is collected after 12h, obtains bacterium solution 1, and measuring its OD600 value with F-2500 sepectrophotofluorometer is 2.068；By abalone luminous bacillus It is seeded in 2216E fluid nutrient medium, 25 DEG C of constant temperature 150r/min oscillation, cultivates and collect bacterium solution after 12h, 10000r/min, 4 DEG C It is centrifuged 20min, abandons supernatant, collects thallus, (EDTA, DTT concentration are divided in the PBS in 0.01mol/L pH=7.0PBS for redissolution Wei 10mmol/L, 1mmol/L), it redissolves than being 2.5g thallus/30ml PBS, obtains bacterium solution 2；
(2) it prepares consolidating fluid: weighing peptone, beef extract, glycerol, NaHCO3、MgSO4, urea and Ca (CH3COO)2, mix Constant volume after adding 1L water to dissolve after conjunction, the final concentration of each substance is respectively peptone 2.0g/L, beef extract 4.0g/L, glycerol 4.0g/ L、NaHCO32.0g/L、MgSO45.0g/L, urea 30.0g/L and Ca (CH3COO)255.0g/L turning in 30 DEG C, 200r/min It is stirred 18h under speed, forms consolidating fluid；
(3) riverway sludge raw material soil 150g is taken, after setting baking oven drying, is crossed spare after 1mm is sieved；
(4) 9mm polypropylene fibre (volume is sequentially added by certain volume proportion in native toward the sieving raw material of step (3) Be the 0.2% of raw material soil property amount), it is 2-3mm grades of high impact polystyrene particles of diameter (volume be raw material soil property amount 1%), straight 1-2mm grades of acrylic resin particles of diameter (volume is the 1% of raw material soil property amount)；
(5) 90g water (volume is the 60% of raw material soil property amount) is added to mixture obtained by step (4), then in blender In stir evenly (setting stirring rate be 110-125r/min, mixing time be 10~20min)；
(6) 4.77mg firefly luciferase, 7.21mg D- luciferin are sequentially added to mixture obtained by step (5), 16.75mg ethylenediamine tetra-acetic acid (EDTA) and 6.94mg dithiothreitol (DTT) (DTT) are sequentially added after mixing evenly；
(7) sample obtained by step (6) is packed into substrate mold, using geotechnological woven fabric, be sewn into diameter is mold The cylinder of the upper opening of 3.9cm, a height of 8cm can delay the loss of bacterium solution and nutrient solution, increase solidification effect.To examination Sample successively bacterium solution 1,30mL bacterium solution 2 obtained by slow trickle irrigation 50mL step (1), stir evenly, by 3 layers of filling of gained sample point, often Reality is hit in layering after layer about 50g sample is packed into sample preparation device, guarantees that specimen surface is smooth.Sample after injection bacterium solution is immersed in 1.5L Consolidating fluid in be put into standard curing box and conserve, curing temperature is (20 ± 2) DEG C, and relative humidity is greater than 95%, after maintenance for 24 hours Demoulding continues to conserve；To get the luminous ecological substrate of the cured high-strength light of microorganism after maintenance to design age 28d.
Embodiment 2:
A kind of luminous ecological substrate of the cured high-strength light of microorganism, preparation method are poly- in addition to being added without in step (4) Other than tacryl, remaining is same as Example 1.
Embodiment 3:
A kind of luminous ecological substrate of the cured high-strength light of microorganism, preparation method is in addition to shining abalone in step (1) Bacillus replaces with other than photobacterium phosphoreum, remaining is same as Example 1.
Embodiment 4:
A kind of luminous ecological substrate of the cured high-strength light of microorganism, preparation method will be in addition to that will be added 9mm in step (4) (volume is raw material soil for 2-3mm grades of polypropylene fibre (volume be raw material soil property amount 1%), diameter high impact polystyrene particles The 5% of quality), other than 1-2mm grades of acrylic resin particles of diameter the 3% of raw material soil property amount (volume be), remaining with implementation Example 1 is identical.
Test example 1:
The luminous ecological substrate of the cured high-strength light of microorganism is made in embodiment 1, is divided using TU-1810 UV, visible light Emission spectrum (the λ that photometric determination is 461nm to transmitting lightB)；Substrate obtained by 1 step of embodiment (7) is taken out into weighing about 60g carries out unconfined compression strength test (with reference to " ecological slope protection theory and technology " certainly), and measuring substrate soil strength is 645kPa meets ecology Gu Po requirement.
Launch wavelength is set as 460nm~462nm, the excitation wavelength within the scope of 220nm~550nm is scanned, exists respectively Occur fluorescence peak at 232nm, 273nm and 342nm, sees Fig. 1.Make excitation wavelength with 232nm, the unstressed configuration at 460nm~462nm Peak.The wherein excitation wavelength that 273nm and 342nm are, it is final to determine that excitation wavelength is 342nm, launch wavelength 461nm, Illustrate to react with D- luciferin can generation wavelength be 461nm fluorescence.
Test example 2:
The luminous ecological substrate of the cured high-strength light of microorganism is made in embodiment 2, is divided using TU-1810 UV, visible light Emission spectrum (the λ that photometric determination is 453nm to transmitting lightB)；Substrate obtained by 2 step of embodiment (7) is taken out into weighing about 59g carries out unconfined compression strength test, and measuring substrate soil strength is 552kPa (intensity reduces by 14.4% on year-on-year basis), meets Ecological Gu Po requirement.
Test example 3:
The luminous ecological substrate of the cured high-strength light of microorganism is made in embodiment 3, is divided using TU-1810 UV, visible light Emission spectrum (the λ that photometric determination is 442nm to transmitting lightB)；Substrate obtained by 3 step of embodiment (7) is taken out into weighing about 60g carries out unconfined compression strength test, and measuring substrate soil strength is 618kPa, meets ecology Gu Po requirement.
Test example 4:
The luminous ecological substrate of the cured high-strength light of microorganism is made in embodiment 4, is divided using TU-1810 UV, visible light Emission spectrum (the λ that photometric determination is 455nm to transmitting lightB)；Substrate obtained by 3 step of embodiment (7) is taken out into weighing about 61g carries out unconfined compression strength test, and measuring substrate soil strength is 634kPa, meets ecology Gu Po requirement.
It should be pointed out that specific embodiment described above can make those skilled in the art that this hair be more fully understood It is bright, but do not limit the invention in any way.Therefore, it will be appreciated by those skilled in the art that still can be carried out to the present invention Modification or equivalent replacement；And all do not depart from the technical solution and its improvement of spirit and technical spirit of the invention, it should all Cover in the scope of protection of the patent of the present invention.

Claims (9)

  1. The preparation method of ecological substrate 1. a kind of cured high-strength light of microorganism shines, its step are as follows:
    (1) preparation solidification microbial inoculum 1 and luminescent microorganism bacterium solution 2；
    The solidification microorganism is Pasteur's sporosarcina and/or Brevibacillus laterosporus；
    The luminescent microorganism is Photobacterium, genus Shewanella and/or Photorhabdus；
    (2) consolidating fluid is prepared；
    The consolidating fluid is by peptone, beef extract, glycerol, NaHCO3、MgSO4, urea and Ca(CH3COO)2It is formed with water；
    (3) after taking raw material soil to set baking oven drying, 1mm sieve is crossed, it is spare；
    (4) toward sequentially adding polypropylene fibre, high impact polystyrene, acrylic resin in the sieving raw material soil of step (3)；
    (5) water is added into mixture obtained by step (4), then stirs evenly；
    (6) earth complex luminescent component is added into mixture obtained by step (5)；
    The earth complex luminescent component are as follows: firefly luciferase, D- luciferin, ethylenediamine tetra-acetic acid and dithiothreitol (DTT)；
    (7) sample, is then immersed in cementing by successively bacterium solution 1, bacterium solution 2 obtained by trickle irrigation step (1) into sample obtained by step (6) Consolidating fluid is sprayed in liquid or into sample, after maintenance, obtains the luminous ecological substrate of the cured high-strength light of microorganism.
  2. 2. preparation method according to claim 1, it is characterised in that: the raw material soil is riverway sludge matter soil.
  3. 3. preparation method according to claim 1, it is characterised in that: the solidification microorganism is that Pasteur's gemma eight folds ball Bacterium.
  4. 4. preparation method according to claim 1, it is characterised in that: the luminescent microorganism be abalone luminous bacillus and/or Photobacterium phosphoreum.
  5. 5. preparation method according to claim 1, it is characterised in that:
    The bacterium solution 1 the preparation method comprises the following steps: by the solidification microbial inoculant to NH4+After cultivating a period of time in-YE culture medium Thallus is collected, bacterium solution 1 is obtained, the OD600 value of bacterium solution 1 is 1.80-2.20.
  6. 6. preparation method according to claim 1, it is characterised in that:
    The bacterium solution 2 the preparation method comprises the following steps: the luminescent microorganism is seeded in 2216E fluid nutrient medium, when cultivating one section Between after collect bacterium solution, then be centrifuged abandoning supernatant, collect thallus, thallus is taken to redissolve in EDTA containing 10mmol/L, 1mmol/L DTT 0.01mol/L, pH=7.0 PBS in, redissolve than be 2.5g thallus/(20-40) ml PBS, obtain bacterium solution 2.
  7. 7. preparation method according to claim 1, it is characterised in that:
    The dosage of bacterium solution 1: raw material soil weight=1mL:(1-6 in step (3) after drying, sieving) g；
    The dosage of bacterium solution 2: raw material soil weight=1mL:(3-8 in step (3) after drying, sieving) g.
  8. 8. any preparation method in -7 according to claim 1, it is characterised in that:
    The substrate each component volume is to account for the mass percentage of the raw material soil after drying in step (3), sieving are as follows:
    Polypropylene fibre 0.2% ~ 1%, high impact polystyrene 1% ~ 5%, acrylic resin 1% ~ 3%, water 50%-70%, earth complex luminescent component 0.2%~0.4%。
  9. 9. any preparation method in -7 according to claim 1, it is characterised in that:
    Firefly luciferase: D- luciferin: EDTA:DTT weight ratio is 1:(1-2): (3-4): (1-2).
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111875306A (en) * 2020-08-10 2020-11-03 中国科学院武汉岩土力学研究所 Microorganism enhancement method for three-dimensional printing of solid cement-based solid model
CN113620616A (en) * 2020-05-08 2021-11-09 博湃建筑科技（上海）有限公司 Method for producing cementing material from Bayer process red mud and concrete admixture
CN115288108A (en) * 2022-09-05 2022-11-04 吉林大学 Self-water-absorbing microorganism curing composite fiber material and preparation method thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1888317A (en) * 2006-07-31 2007-01-03 潘英豪 Illuminating light reflective concrete curb and producing method thereof
CN105040675A (en) * 2015-09-14 2015-11-11 东南大学 Sandy soil microbe solidification method and apparatus
CN109900880A (en) * 2019-03-25 2019-06-18 贵州大学 A kind of MICP test method using immobilized microorganism technique

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1888317A (en) * 2006-07-31 2007-01-03 潘英豪 Illuminating light reflective concrete curb and producing method thereof
CN105040675A (en) * 2015-09-14 2015-11-11 东南大学 Sandy soil microbe solidification method and apparatus
CN109900880A (en) * 2019-03-25 2019-06-18 贵州大学 A kind of MICP test method using immobilized microorganism technique

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
赵述智等: "《实用建筑材料试验手册》", 31 March 1998, 北京：中国建筑工业出版社 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113620616A (en) * 2020-05-08 2021-11-09 博湃建筑科技（上海）有限公司 Method for producing cementing material from Bayer process red mud and concrete admixture
CN113620616B (en) * 2020-05-08 2023-10-17 博湃建筑科技（上海）有限公司 Method for producing cementing material from Bayer process red mud and concrete admixture
CN111875306A (en) * 2020-08-10 2020-11-03 中国科学院武汉岩土力学研究所 Microorganism enhancement method for three-dimensional printing of solid cement-based solid model
CN111875306B (en) * 2020-08-10 2021-12-07 中国科学院武汉岩土力学研究所 Microorganism enhancement method for three-dimensional printing of solid cement-based solid model
CN115288108A (en) * 2022-09-05 2022-11-04 吉林大学 Self-water-absorbing microorganism curing composite fiber material and preparation method thereof

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