CN110214970B - Tobacco leaf enzyme treatment process and system - Google Patents
Tobacco leaf enzyme treatment process and system Download PDFInfo
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- CN110214970B CN110214970B CN201910464302.7A CN201910464302A CN110214970B CN 110214970 B CN110214970 B CN 110214970B CN 201910464302 A CN201910464302 A CN 201910464302A CN 110214970 B CN110214970 B CN 110214970B
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- 235000002637 Nicotiana tabacum Nutrition 0.000 title claims abstract description 200
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 146
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 146
- 238000000034 method Methods 0.000 title claims abstract description 85
- 244000061176 Nicotiana tabacum Species 0.000 title 1
- 241000208125 Nicotiana Species 0.000 claims abstract description 199
- 238000002360 preparation method Methods 0.000 claims abstract description 53
- 238000002791 soaking Methods 0.000 claims abstract description 50
- 238000006243 chemical reaction Methods 0.000 claims abstract description 28
- 239000012295 chemical reaction liquid Substances 0.000 claims description 17
- 230000002779 inactivation Effects 0.000 claims description 13
- 230000005540 biological transmission Effects 0.000 claims description 8
- 239000003480 eluent Substances 0.000 claims description 7
- 238000010828 elution Methods 0.000 claims description 5
- 238000005507 spraying Methods 0.000 claims description 5
- 238000005119 centrifugation Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 14
- 229940088598 enzyme Drugs 0.000 description 124
- 239000000523 sample Substances 0.000 description 20
- 238000001514 detection method Methods 0.000 description 9
- 239000000126 substance Substances 0.000 description 9
- 230000002411 adverse Effects 0.000 description 8
- SNICXCGAKADSCV-JTQLQIEISA-N (-)-Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 description 7
- 229960002715 nicotine Drugs 0.000 description 7
- SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 description 7
- 239000013068 control sample Substances 0.000 description 6
- 238000011156 evaluation Methods 0.000 description 6
- 230000001953 sensory effect Effects 0.000 description 6
- 108091005508 Acid proteases Proteins 0.000 description 5
- 229920002472 Starch Polymers 0.000 description 5
- 230000007613 environmental effect Effects 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 235000019698 starch Nutrition 0.000 description 5
- 239000008107 starch Substances 0.000 description 5
- 108010059892 Cellulase Proteins 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 4
- 229940106157 cellulase Drugs 0.000 description 4
- 239000001913 cellulose Substances 0.000 description 4
- 229920002678 cellulose Polymers 0.000 description 4
- 235000019504 cigarettes Nutrition 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 229920001277 pectin Polymers 0.000 description 3
- 230000000704 physical effect Effects 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 108010065511 Amylases Proteins 0.000 description 2
- 102000013142 Amylases Human genes 0.000 description 2
- 108091005507 Neutral proteases Proteins 0.000 description 2
- 102000035092 Neutral proteases Human genes 0.000 description 2
- 235000019418 amylase Nutrition 0.000 description 2
- 239000000084 colloidal system Substances 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000007794 irritation Effects 0.000 description 2
- 239000001814 pectin Substances 0.000 description 2
- 235000010987 pectin Nutrition 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 1
- 108090000145 Bacillolysin Proteins 0.000 description 1
- 108010084185 Cellulases Proteins 0.000 description 1
- 102000005575 Cellulases Human genes 0.000 description 1
- 108010059820 Polygalacturonase Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 229940025131 amylases Drugs 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical compound OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 229940079919 digestives enzyme preparation Drugs 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 108010093305 exopolygalacturonase Proteins 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 210000003701 histiocyte Anatomy 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000019505 tobacco product Nutrition 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/20—Biochemical treatment
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
- A24B3/18—Other treatment of leaves, e.g. puffing, crimpling, cleaning
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Manufacture Of Tobacco Products (AREA)
Abstract
The invention discloses a tobacco leaf enzyme treatment process and a system, wherein the process comprises the following steps: soaking treatment: soaking tobacco leaves in an enzyme preparation reaction solution for 10s-2 min; draining: draining the soaked tobacco leaves and caching for a period of time, wherein the moisture content of the tobacco leaves is reduced to be less than or equal to 25% after draining treatment; and (3) centrifugal treatment: centrifuging the drained tobacco leaves until the moisture content is 16% -19%; and (3) storage treatment: storing the centrifuged tobacco leaves for a period of time. According to the tobacco leaf enzyme treatment process, the tobacco leaves are sequentially subjected to soaking treatment, draining treatment, centrifugal treatment and storage treatment, so that the enzyme preparation is ensured to be fully contacted and fully reacted with the tobacco leaves, the process time can be effectively shortened, the enzyme treatment effect is improved, and the quality of the tobacco leaves is effectively improved.
Description
Technical Field
The invention relates to the field of tobacco threshing and redrying processing, in particular to a tobacco leaf enzyme treatment process and a tobacco leaf enzyme treatment system.
Background
The aroma and taste of tobacco have a significant impact on the quality of tobacco products. In the threshing and redrying process, the quality of tobacco leaves is improved and enhanced by adopting an enzyme feeding technology. Common enzyme adding technologies can be divided into two types, namely adding enzyme before the leaf redrying process and adding enzyme after the leaf redrying process.
For the process of adding enzyme before the leaf redrying procedure, the existing tobacco leaf enzyme treatment process is as follows: and (3) feeding the tobacco flakes subjected to threshing and air separation into a conventional enzyme adding machine, spraying the diluted enzyme preparation onto the tobacco flakes in an atomized form, storing for a period of time, and then feeding into a tobacco leaf redrying machine for redrying.
Because a large amount of colloid and waxy components are attached to the surface of the leaf, the enzyme preparation is not easy to enter the tobacco cells to react. Moreover, the atomized enzyme preparation is difficult to be in full contact with tobacco leaves, and the contact effect is poor, so that the time of the tobacco enzyme treatment process is as long as more than four hours. Based on the defects of the enzyme treatment process, the existing tobacco leaf enzyme treatment process has long time and poor effect, and the quality of tobacco leaves is difficult to effectively improve.
Therefore, how to provide a tobacco leaf enzyme treatment process which has good effect and can effectively shorten the process time and improve the quality of tobacco leaves becomes a technical problem which needs to be solved urgently in the field.
Disclosure of Invention
The invention aims to provide a new technical scheme of the tobacco leaf enzyme treatment process, which has good effect and can effectively shorten the process time and improve the quality of tobacco leaves.
According to a first aspect of the invention, there is provided a tobacco leaf enzyme treatment process.
The tobacco leaf enzyme treatment process comprises the following steps:
soaking treatment: soaking tobacco leaves in an enzyme preparation reaction solution for 10s-2 min;
draining: draining the soaked tobacco leaves and caching for a period of time so that the moisture content of the tobacco leaves is reduced to be less than or equal to 25%;
and (3) centrifugal treatment: centrifuging the drained tobacco leaves until the moisture content is 16% -19%;
and (3) storage treatment: storing the centrifuged tobacco leaves for a period of time.
Optionally, the concentration of the enzyme preparation reaction solution adopted in the soaking treatment is 0.03 wt% -1.0 wt%, and the pH value of the enzyme preparation reaction solution is 2.0-8.0.
Optionally, the process temperature of the soaking treatment is 35-65 ℃.
Optionally, the soaking time of the soaking treatment is 30s-1 min.
Optionally, the process time of the draining treatment is 20min to 30 min.
Optionally, the rotation speed of the centrifugal treatment is 700r/min-800r/min, and the time is 2min-4 min.
Optionally, the storage treatment process temperature is 30-60 ℃, the ambient humidity is 50-80%, and the storage treatment time is 30min-1 h.
Optionally, before the soaking treatment, an elution treatment is further included, specifically as follows:
and spraying an eluent on the surface of the tobacco leaves.
Optionally, the storage treatment further comprises inactivation treatment, specifically as follows:
and (4) carrying out enzyme inactivation treatment on the stored tobacco leaves.
According to a second aspect of the present invention, there is provided a tobacco leaf enzyme treatment system for carrying out the tobacco leaf enzyme treatment process of the present invention.
The tobacco enzyme treatment system comprises a soaking tank, a transmission device, a centrifugal device and a storage device; wherein,
an enzyme preparation reaction liquid for soaking tobacco leaves is arranged in the soaking tank;
one end of the transmission device is positioned at the bottom of the soaking tank, and the other end of the transmission device is connected with the centrifugal device;
the centrifugal device is arranged for centrifuging the tobacco leaves conveyed by the conveying device;
the storage device is configured for storing tobacco leaves.
According to the tobacco leaf enzyme treatment process, the tobacco leaves are sequentially subjected to soaking treatment, draining treatment, centrifugal treatment and storage treatment, so that the enzyme preparation is ensured to be fully contacted and fully reacted with the tobacco leaves, the process time can be effectively shortened, the enzyme treatment effect is improved, and the quality of the tobacco leaves is effectively improved.
Other features of the present invention and advantages thereof will become apparent from the following detailed description of exemplary embodiments thereof, which proceeds with reference to the accompanying drawings.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description, serve to explain the principles of the invention.
FIG. 1 is a flow chart of an embodiment of the tobacco leaf enzyme treatment process of the present disclosure.
Detailed Description
Various exemplary embodiments of the present invention will now be described in detail with reference to the accompanying drawings. It should be noted that: the relative arrangement of the components and steps, the numerical expressions and numerical values set forth in these embodiments do not limit the scope of the present invention unless specifically stated otherwise.
The following description of at least one exemplary embodiment is merely illustrative in nature and is in no way intended to limit the invention, its application, or uses.
Techniques, methods, and apparatus known to those of ordinary skill in the relevant art may not be discussed in detail but are intended to be part of the specification where appropriate.
In all examples shown and discussed herein, any particular value should be construed as merely illustrative, and not limiting. Thus, other examples of the exemplary embodiments may have different values.
As shown in fig. 1, the tobacco leaf enzyme treatment process of the present disclosure includes the following steps:
soaking treatment: soaking tobacco leaf in enzyme preparation reaction solution for 10s-2 min.
Too short soaking time of the tobacco leaves in the enzyme preparation reaction liquid can cause the tobacco leaves not to be fully contacted with the enzyme preparation reaction liquid, and too long soaking time can cause the moisture content of the tobacco leaves to be too large, thereby having adverse effect on subsequent treatment. In addition, too long soaking time can affect the content of chemical components (such as nicotine) in the tobacco leaves, so that the quality of the tobacco leaves is not easy to control. Therefore, the tobacco leaves are soaked in the enzyme preparation reaction liquid for 10s-2min, so that the tobacco leaves are favorably and fully contacted with the enzyme preparation reaction liquid, and the adverse effects on the subsequent treatment and the quality of the tobacco leaves are avoided.
Further, the soaking time can be controlled within 30s-1 min.
The concentration of the enzyme preparation reaction solution and the soaking process parameters can influence the enzyme treatment effect of the tobacco leaves.
Common enzyme preparations include acid proteases, neutral proteases, cellulases, pectic enzymes, saccharifying enzymes, amylases, and complex enzymes comprising at least two of the foregoing enzymes. Generally, the concentration of the enzyme preparation reaction solution used in the immersion treatment may be 0.03 wt% to 1.0 wt%, and the pH of the enzyme preparation reaction solution may be 2.0 to 8.0. The process temperature of the soaking treatment can be 35-65 ℃. Further, the concentration of the enzyme preparation reaction liquid adopted in the soaking treatment can be 0.03 wt% -0.08 wt%, the pH value of the enzyme preparation reaction liquid can be 2.0-7.5, and the process temperature of the soaking treatment can be 40-60 ℃.
For acid protease, the process temperature of the soaking treatment can be 40-60 ℃, the pH value of the enzyme preparation reaction liquid can be 2.0-4.0, and the concentration of the enzyme preparation reaction liquid can be 0.05 wt%.
For neutral protease, the process temperature of the soaking treatment can be 40-60 ℃, the pH value of the enzyme preparation reaction liquid can be 5.5-7.5, and the concentration of the enzyme preparation reaction liquid can be 0.07 wt%.
For the cellulase, the process temperature of the soaking treatment may be 50 ℃, the pH of the enzyme preparation reaction solution may be 5.0, and the concentration of the enzyme preparation reaction solution may be 0.08 wt%.
For amylase, the process temperature for the soaking treatment may be 50 ℃, the pH of the enzyme preparation reaction solution may be 7.0, and the concentration of the enzyme preparation reaction solution may be 0.03 wt%.
Draining: and draining the soaked tobacco leaves and caching for a period of time, wherein the moisture content of the tobacco leaves is reduced to be less than or equal to 25% after draining treatment.
The time of the draining treatment affects the contact time of the enzyme preparation reaction liquid on the surface of the tobacco leaves and the tobacco leaves, too short draining time can cause that the redundant enzyme preparation reaction liquid cannot be drained from the tobacco leaves, and too long draining time can affect the content of chemical components (such as nicotine) in the tobacco leaves, cause tobacco leaves to be bonded and affect the quality of the tobacco leaves. Therefore, the process time of the draining treatment is controlled to be 20min-30min, which is beneficial to fully contacting the tobacco leaves with the enzyme preparation reaction liquid and does not generate adverse effect on the quality of the tobacco leaves.
And (3) centrifugal treatment: and centrifuging the drained tobacco leaves until the moisture content is 16-19%.
The tobacco leaves after the centrifugal treatment are stored, and the stored tobacco leaves enter a redrying procedure. Too high a moisture content of the centrifuged tobacco leaves will adversely affect the storage process and the redrying process. Therefore, controlling the moisture content of the centrifuged tobacco leaves to 16% to 19% can have a favorable effect on the subsequent processes.
In addition, the rotation speed and time of the centrifugal treatment can affect the physical properties of the tobacco leaves, such as flexibility and the like, so that the rotation speed and time of the centrifugal treatment need to be reasonably matched so as to reduce the moisture content of the tobacco leaves to 16% -19% and simultaneously not to bring adverse effects on the physical properties of the tobacco leaves. The rotation speed of the centrifugal treatment is controlled to be 700r/min-800r/min, and the time is controlled to be 2min-4min, so that the moisture content of the tobacco leaves can be reduced, and the stability of the physical properties of the tobacco leaves can be ensured.
And (3) storage treatment: storing the centrifuged tobacco leaves for a period of time to enable the enzyme preparation to fully react with the tobacco leaves.
The storage treatment is beneficial to the full reaction of the enzyme preparation and the tobacco leaves. The temperature of the storage treatment process is controlled to be 30-60 ℃, the environmental humidity is controlled to be 50-80%, and the time is controlled to be 30min-1h, so that the full reaction of the enzyme preparation and the tobacco leaves is facilitated, and the adverse effect on the properties of the tobacco leaves is not brought. Furthermore, the process temperature of the storage treatment can be controlled to be 40-60 ℃, and the environmental humidity can be controlled to be 60-80%.
The tobacco leaf enzyme treatment process disclosed by the invention can be carried out after tobacco leaves are subjected to threshing and air separation. The tobacco leaves obtained after the tobacco leaf enzyme treatment process can be subjected to redrying treatment.
According to the tobacco leaf enzyme treatment process, the tobacco leaves are sequentially subjected to soaking treatment, draining treatment, centrifugal treatment and storage treatment, so that the enzyme preparation is ensured to be fully contacted and fully reacted with the tobacco leaves, the process time can be effectively shortened, the enzyme treatment effect is improved, and the quality of the tobacco leaves is effectively improved.
In one embodiment of the tobacco leaf enzyme treatment process of the present disclosure, an elution process is further included before the soaking process. The elution process was specifically as follows: and spraying an eluent on the surface of the tobacco leaves.
The eluent can perform the elution function on the colloid and the wax on the surface of the tobacco leaves, and is more beneficial to the enzyme preparation to enter the histiocyte of the tobacco leaves for reaction. A common eluent may for example be a weak acid (e.g. carbonic acid). In order to avoid that excessive eluent has adverse effect on the tobacco leaves, the eluent can be dispersed on the surface of the tobacco leaves by means of spraying.
In one embodiment of the tobacco leaf enzyme treatment process of the present disclosure, the storage treatment further comprises an inactivation treatment. The inactivation treatment is specifically as follows: and (4) carrying out enzyme inactivation treatment on the stored tobacco leaves.
The enzyme preparation may be inactivated during the redrying process, but the temperature of the redrying process is high and the time is long, and the enzyme preparation may react with the chemical substances in the tobacco leaves in an adverse way during the redrying process. Therefore, after the storage treatment, the tobacco leaves can be subjected to enzyme inactivation treatment before the tobacco leaves enter the redrying process. The inactivation treatment in the tobacco leaf enzyme treatment process disclosed by the invention is high-temperature rapid inactivation treatment. In specific implementation, the process parameters of the inactivation treatment can be as follows: the temperature is 70-75 ℃ and the time is 5-10 s. The inactivation rate of the enzyme preparation in the tobacco leaves after inactivation treatment can reach 30-60%, which is beneficial to the improvement of the quality of the tobacco leaves in the subsequent redrying process.
The present disclosure also provides a tobacco leaf enzyme treatment system.
The tobacco leaf enzyme treatment system comprises a soaking tank, a transmission device, a centrifugal device and a storage device.
The soaking tank is internally provided with enzyme preparation reaction liquid for soaking tobacco leaves and can be used for a soaking treatment process of a tobacco leaf enzyme treatment process.
One end of the transmission device is positioned at the bottom of the soaking tank, and the other end of the transmission device is connected with the centrifugal device. The conveying device can convey the tobacco leaves in the soaking tank to the centrifugal device. The conveying device not only can convey the tobacco leaves, but also can be used for implementing the draining treatment process of the tobacco leaf enzyme treatment process. The transport device may be a conveyor belt, for example.
The centrifugal device may be used to centrifuge tobacco leaves transported by the conveying device. The centrifugal device can be used for implementing the centrifugal treatment process of the tobacco leaf enzyme treatment process. The centrifugation device may for example be a centrifuge.
The storage device may be used to store tobacco leaves. The storage device can be used for implementing the storage treatment process of the tobacco leaf enzyme treatment process. The storage device may for example be a leaf locker.
The experimental procedures used in the examples below are conventional unless otherwise specified, the materials and reagents used therein are commercially available, and the equipment used in the experiments are well known to those skilled in the art without otherwise specified.
Example 1
100kg of tobacco leaves (2018/flue-cured tobacco/Yunnan Dali/Hongda B1F) are soaked in the enzyme preparation reaction solution for 30 s. Wherein the enzyme preparation reaction solution is an acid protease reaction solution, the process temperature of the soaking treatment is 50 ℃, the pH value of the enzyme preparation reaction solution is 4.0, and the concentration of the acid protease is 0.07 wt%. And (3) draining the soaked tobacco leaves and caching for 20min, wherein the moisture content of the tobacco leaves is reduced to 20% after draining treatment. And (4) centrifuging the drained tobacco leaves until the moisture content is 18%, wherein the rotation speed of the centrifugal treatment is 700r/min, and the time is 2 min. And (3) storing the centrifuged tobacco leaves for 30min at the temperature of 50 ℃ and the environmental humidity of 60% to obtain an enzyme-treated tobacco leaf sample S1.
The chemical components of the enzyme-treated tobacco leaf sample S1 were measured, and the tobacco leaves without enzyme treatment were used as a control, and the measurement results are shown in the following table:
TABLE 1 tobacco leaf chemical composition detection data sheet
| Test sample | Total sugar wt% | Nicotine wt.% | Starch wt% | Protein wt.% |
| S1 | 31.993 | 3.846 | 5.8 | 4.056 |
| Control sample | 32.433 | 3.857 | 5.4 | 4.943 |
As can be seen from Table 1, the total sugar, starch and nicotine contents of the enzyme-treated tobacco leaf sample S1 and the tobacco leaves without enzyme treatment are different, and the protein content difference between the enzyme-treated tobacco leaf sample S1 and the tobacco leaves without enzyme treatment is more obvious.
Preparing a single tobacco (finished tobacco lamina) from the enzyme-treated tobacco sample S1, and performing sensory evaluation detection by taking the single tobacco prepared from the tobacco which is not subjected to enzyme treatment as a control sample, wherein the detection results are as follows:
TABLE 2 comparison of sensory evaluation results for single cigarette
As can be seen from table 2, the enzyme-treated tobacco sample S1 was improved in aroma and irritation and improved in oral comfort compared to the non-enzyme-treated tobacco.
Example 2
100kg of tobacco leaves (2018/flue-cured tobacco/Yunnan Kunming/Hongda B2F) are soaked in the enzyme preparation reaction liquid for 1 min. Wherein the enzyme preparation reaction solution is a cellulase reaction solution, the process temperature of the soaking treatment is 50 ℃, the pH value of the enzyme preparation reaction solution is 5, and the concentration of the cellulase is 0.08 wt%. And (3) draining the soaked tobacco leaves and caching for 30min, wherein the moisture content of the tobacco leaves is reduced to 20% after draining treatment. And (4) centrifuging the drained tobacco leaves until the moisture content is 18%, wherein the rotation speed of the centrifugal treatment is 800r/min, and the time is 3 min. And (4) storing the centrifuged tobacco leaves for 40min at the temperature of 50 ℃ and the environmental humidity of 70% to obtain an enzyme-treated tobacco leaf sample S2.
The chemical components of the enzyme-treated tobacco leaf sample S2 were measured, and the tobacco leaves without enzyme treatment were used as a control, and the measurement results are shown in the following table:
TABLE 3 tobacco leaf chemical composition detection data sheet
| Test sample | Total sugar wt% | Nicotine wt.% | Starch wt% | Protein wt.% | Cellulose wt.% |
| S2 | 31.99 | 3.75 | 5.73 | 4.056 | 9.02 |
| Control sample | 31.80 | 3.69 | 5.69 | 4.943 | 9.71 |
As can be seen from Table 3, the total sugar, starch, protein and nicotine contents of the enzyme-treated tobacco leaf sample S2 and the non-enzyme-treated tobacco leaf were different, and the cellulose content difference between the enzyme-treated tobacco leaf sample S2 and the non-enzyme-treated tobacco leaf was significant.
Preparing a single tobacco (finished tobacco lamina) from the enzyme-treated tobacco sample S2, and performing sensory evaluation detection by taking the single tobacco prepared from the tobacco which is not subjected to enzyme treatment as a control sample, wherein the detection results are as follows:
TABLE 4 comparison of sensory evaluation results for single cigarette
As can be seen from Table 4, the enzyme-treated tobacco sample S2 was improved in aroma and offensive odor compared to non-enzyme-treated tobacco.
Example 3
100kg of tobacco leaves are soaked in the enzyme preparation reaction solution for 40 s. Wherein the enzyme preparation reaction solution is a composite enzyme reaction solution of acid protease, cellulase and pectinase in a mass ratio of 1:1:1, the soaking temperature is 50 ℃, the pH value of the enzyme preparation reaction solution is 7, and the concentration of the composite enzyme is 0.03 wt%. Draining the soaked tobacco leaves and caching for 25min, wherein the moisture content of the tobacco leaves is reduced to 20% after draining treatment. And (4) centrifuging the drained tobacco leaves until the moisture content is 18%, wherein the rotation speed of the centrifugal treatment is 750r/min, and the time is 2.5 min. And (3) storing the centrifuged tobacco leaves for 50min at the temperature of 50 ℃ and the environmental humidity of 65% to obtain an enzyme-treated tobacco leaf sample S3.
The chemical components of the enzyme-treated tobacco leaf sample S3 were measured, and the tobacco leaves without enzyme treatment were used as a control, and the measurement results are shown in the following table:
TABLE 5 tobacco leaf chemical composition detection data sheet
| Test sample | Total sugar wt% | Nicotine wt.% | Pectin wt.% | Starch wt% | Protein wt.% | Cellulose wt.% |
| S3 | 32.767 | 3.68 | 14.667 | 8.017 | 4.983 | 9.127 |
| Control sample | 30.073 | 3.74 | 15.067 | 7.987 | 5.070 | 9.600 |
As can be seen from Table 5, the total sugar of the enzyme-treated tobacco sample S3 was significantly increased and the cellulose, pectin and protein were reduced compared to the non-enzyme-treated tobacco.
Preparing a single tobacco (finished tobacco lamina) from the enzyme-treated tobacco sample S3, and performing sensory evaluation detection by taking the single tobacco prepared from the tobacco which is not subjected to enzyme treatment as a control sample, wherein the detection results are as follows:
TABLE 6 comparison of sensory evaluation results for single cigarette
As can be seen from Table 6, the enzyme-treated tobacco sample S3 was improved in aroma quality, aroma quantity and irritation as compared to non-enzyme-treated tobacco.
Although some specific embodiments of the present invention have been described in detail by way of examples, it should be understood by those skilled in the art that the above examples are for illustrative purposes only and are not intended to limit the scope of the present invention. It will be appreciated by those skilled in the art that modifications may be made to the above embodiments without departing from the scope and spirit of the invention. The scope of the invention is defined by the appended claims.
Claims (10)
1. The tobacco leaf enzyme treatment process is characterized by comprising the following steps:
soaking treatment: soaking tobacco leaves in an enzyme preparation reaction solution for 10s-2 min;
draining: draining the soaked tobacco leaves and caching for a period of time, wherein the moisture content of the tobacco leaves is reduced to be less than or equal to 25% after draining treatment;
and (3) centrifugal treatment: centrifuging the drained tobacco leaves until the moisture content is 16% -19%;
and (3) storage treatment: storing the centrifuged tobacco leaves for a period of time.
2. The tobacco leaf enzyme treatment process according to claim 1, wherein the concentration of the enzyme preparation reaction solution adopted in the soaking treatment is 0.03 wt% -1.0 wt%, and the pH value of the enzyme preparation reaction solution is 2.0-8.0.
3. The tobacco leaf enzyme treatment process according to claim 2, wherein the process temperature of the soaking treatment is 35 ℃ to 65 ℃.
4. The tobacco leaf enzyme treatment process according to claim 1, wherein the soaking time of the soaking treatment is 30s-1 min.
5. The tobacco leaf enzyme treatment process according to claim 1, wherein the process time of the draining treatment is 20min-30 min.
6. The tobacco leaf enzyme treatment process according to claim 1, wherein the rotation speed of the centrifugal treatment is 700r/min-800r/min, and the time is 2min-4 min.
7. The tobacco leaf enzyme treatment process according to claim 1, wherein the storage treatment process temperature is 30-60 ℃, the ambient humidity is 50-80%, and the time is 30min-1 h.
8. The tobacco leaf enzyme treatment process according to any one of claims 1 to 7, further comprising an elution treatment before the soaking treatment, in particular as follows:
and spraying an eluent on the surface of the tobacco leaves.
9. The tobacco leaf enzyme treatment process according to any one of claims 1 to 7, further comprising an inactivation treatment after the storage treatment, in particular as follows:
and (4) carrying out enzyme inactivation treatment on the stored tobacco leaves.
10. A tobacco leaf enzyme treatment system for carrying out the tobacco leaf enzyme treatment process according to any one of claims 1 to 9, comprising a steeping cistern, a conveying means, a centrifugation means and a storage means; wherein,
an enzyme preparation reaction liquid for soaking tobacco leaves is arranged in the soaking tank;
one end of the transmission device is positioned at the bottom of the soaking tank, and the other end of the transmission device is connected with the centrifugal device;
the centrifugal device is arranged for centrifuging the tobacco leaves conveyed by the conveying device;
the storage device is configured for storing tobacco leaves.
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