CN110208076B - Digestion device for amino acid digestion experiment - Google Patents
Digestion device for amino acid digestion experiment Download PDFInfo
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- CN110208076B CN110208076B CN201910515038.5A CN201910515038A CN110208076B CN 110208076 B CN110208076 B CN 110208076B CN 201910515038 A CN201910515038 A CN 201910515038A CN 110208076 B CN110208076 B CN 110208076B
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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Abstract
The invention discloses a digestion device for an amino acid digestion experiment, and particularly relates to a laboratory instrument, which comprises an accommodating inner cylinder, a solid-liquid mixing mechanism and an outer sleeve; the solid-liquid mixing mechanism comprises a fixing column, a first elastic piece, a mixing barrel and a storage mechanism; the bottom end of the blending cylinder is provided with a through hole which is matched with the fixed column to penetrate through, the first elastic piece is sleeved on the outer side wall of the fixed column, one end of the first elastic piece is fixed with the bottom end of the containing inner cylinder, and the other end of the first elastic piece is fixed with the bottom of the blending cylinder; the opening end of the outer sleeve is provided with an opening and closing mechanism for sealing gas or ventilating, the accommodating inner cylinder and the solid-liquid mixing mechanism are both arranged inside the outer sleeve, one side of the outer sleeve, which faces away from the opening end, is provided with a joint communicated with the nitrogen vent pipe, correspondingly, the side wall of the accommodating inner cylinder is provided with a nitrogen gas outflow hole for nitrogen gas to flow out of the outer sleeve, and a gap for nitrogen gas to pass through is reserved between the accommodating inner cylinder and the outer sleeve. The invention has the advantage of being convenient for experimenters to realize homogenization through oscillation.
Description
Technical Field
The invention relates to a laboratory instrument, in particular to a digestion device for an amino acid digestion experiment.
Background
When the elements in the sample are measured, the sample needs to be digested. The digestion treatment has the functions of destroying organic matters, dissolving particulate matters and oxidizing elements to be detected in various valence states into single high valence state or converting the elements into inorganic compounds easy to decompose.
Wherein, in the experimental process of digesting the amino acid, the digestion is carried out in an anaerobic environment. It is common practice for the experimenter to add the sample and reagents to a small glass tube and to introduce a sufficient amount of nitrogen gas into the glass tube to expel the air, which is then sealed with an alcohol torch. However, in the above experimental process, when the sample powder is added into the glass tube, it is often difficult for the middle portion of the sample powder to sufficiently contact with the reagent, thereby affecting the digestion effect.
Disclosure of Invention
The invention provides a digestion device for an amino acid digestion experiment, which has the advantage of facilitating experimenters to realize homogenization through oscillation.
In order to achieve the purpose, the invention provides the following technical scheme:
a digestion device for an amino acid digestion experiment comprises an accommodating inner cylinder, a solid-liquid mixing mechanism and an outer sleeve; the solid-liquid mixing mechanism comprises a fixing column, a first elastic piece, a mixing cylinder and a storage mechanism for storing reagents, wherein the fixing column is detachably connected with the lower end of the accommodating inner cylinder; the bottom end of the blending cylinder is provided with a through hole matched with the fixing column to penetrate through, the first elastic piece is sleeved on the outer side wall of the fixing column, one end of the first elastic piece is fixed with the bottom end of the containing inner cylinder, and the other end of the first elastic piece is fixed with the bottom of the blending cylinder; the open end of outer sleeve is equipped with the start-stop gear that sealed gas or ventilate, it all sets up in the inside of outer sleeve to hold inner tube and solid-liquid mixing mechanism, outer sleeve open end one side dorsad is equipped with the joint with the switch-on of nitrogen gas breather pipe, correspondingly, the lateral wall that holds the inner tube is equipped with the nitrogen gas stream that supplies the nitrogen gas outflow to get into the outer sleeve and exports, holds and leaves the clearance that supplies the nitrogen gas to pass through between inner tube and the outer sleeve.
Preferably, one end of the fixing column, which faces away from the accommodating inner cylinder, is provided with a fixing end socket; the storage mechanism comprises a connecting column, a connecting rod, a hollow positioning sleeve with two open ends and a liquid storage cylinder; one end of the connecting column is detachably connected with the fixed end, the other end of the connecting column is fixed with the connecting rod, and one end of the connecting rod, which is back to the connecting column, is fixed with the outer side wall of the positioning sleeve; the outer sleeve comprises a top part, the liquid storage cylinder is fixed with the inward side of the top part, and the liquid storage cylinder slides in the hollow part of the positioning sleeve; the storage mechanism also comprises an opening and closing piece which is used for controlling the reagent to flow out along with the up-and-down shaking of the blending cylinder.
Preferably, the opening and closing piece comprises a positioning disc, a push rod, a second elastic piece and a sealing disc, wherein the positioning disc is arranged at one end, back to the liquid storage cylinder, of the positioning sleeve; the middle part of the positioning disc is fixed with the ejector rod, and the positioning disc is provided with a liquid outlet groove for the reagent to flow out; correspondingly, the liquid storage barrel is equipped with the spacing ring towards ejector pin one side, the top fixedly connected with second elastic component of spacing ring, the one end and the sealed disc of second elastic component spacing ring dorsad are fixed mutually, the diameter of sealed disc is less than the liquid storage barrel.
Preferably, one end of the liquid storage cylinder, which faces away from the sealing disc, is provided with a liquid injection through hole, and the liquid injection through hole is provided with a plug.
Preferably, the outer sleeve further comprises a side part and a columnar connecting part, one end of the side part is fixed with the top, the other end of the side part is detachably connected with the connecting part, and a sealing plate is fixedly arranged on the inner side of the connecting part; the opening and closing mechanism comprises a connecting rod and a sliding sleeve ring, wherein the connecting rod and the sliding sleeve ring are fixed with the lower end of the sealing plate; the sealing plate is provided with a nitrogen gas outflow groove, and the wall of the nitrogen gas outflow groove is connected with an airflow sealing plate in a unilateral rotating manner; the upper end of the sliding lantern ring is provided with a jacking rod which jacks up the airflow sealing plate to reserve a gap for nitrogen to pass through.
Preferably, the digestion device further comprises a detection mechanism for detecting whether the air in the inner area of the outer sleeve is exhausted, the detection mechanism comprises an air storage cylinder which is detachably connected with the connecting part, a fixing plate is arranged at the opening position of the air storage cylinder, a nitrogen through groove is formed in the fixing plate, a through hole which is matched with the connecting rod to penetrate is formed in the fixing plate, and the air storage cylinder is made of transparent materials.
Preferably, an indication slide plate is slidably provided in the air reservoir.
Preferably, the junction of the side part and the top part is provided with an arc-shaped fixing part which is suitable for accommodating the insertion of the inner cylinder.
Preferably, the mixing cylinder is provided with a plurality of circular holes through which the mixture of the sample and the reagent passes.
Preferably, the one end of fixed column dorsad fixed end is equipped with the stationary blade, correspondingly, the bottom of holding the inner tube is equipped with the caulking groove that the adaptation stationary blade rotated the back and imbeds.
The invention has the beneficial effects that:
in the present invention, the experimenter may place the sample on the mixing drum and inject the reagents into the storage mechanism. Because the fixed end head and the bottom end of the mixing cylinder are arranged in a sliding way. So the experiment personnel accessible rocks the outer sleeve from top to bottom for mixing section of thick bamboo moves in vertical direction, and with store the liquid of flowing out in the mechanism and carry out the mixing. When the sample and the reagent are mixed uniformly, the mixture flows into the bottom of the containing inner cylinder to wait for digestion reaction. Because the amino acid digestion reaction is anaerobic, an experimenter can introduce nitrogen through the joint and discharge the nitrogen from the nitrogen outlet hole into the outer sleeve along the gap between the inner containing cylinder and the outer sleeve. And finally, discharging the nitrogen through the opening and closing mechanism, and performing a microwave digestion experiment when the air in the outer sleeve is exhausted. By using the digestion device provided by the invention, an experimenter can fully mix the sample and the reagent, so that the digestion effect is better.
Drawings
FIG. 1 is a schematic structural diagram of a digestion apparatus for an amino acid digestion experiment in the present example;
FIG. 2 is a schematic sectional view of the digestion apparatus for an amino acid digestion experiment in the present embodiment;
FIG. 3 is an enlarged view of portion A of FIG. 2;
fig. 4 is an enlarged view of a portion B in fig. 2.
In the figure: 1. mixing barrel, 2, hold the inner tube, 21, nitrogen gas outlet hole, 31, the end cap, 33, the liquid storage barrel, 34, sealing disc, 35, the second elastic component, 36, the lateral part, 37, the top, 370, the arc fixed part, 38, the caulking groove, 4, the connecting part, 41, the airflow sealing plate, 42, the sealing plate, 43, the connecting rod, 44, the slip lantern ring, 440, the jack-up pole, 5, the gas receiver, 51, the fixed plate, 52, the nitrogen gas through groove, 53, the perforation, 61, the fixed column, 62, the first elastic component, 64, the fixed end, 65, the spliced pole, 66, the connecting rod, 67, the position sleeve, 68, the positioning disk, 69, the ejector pin.
Detailed Description
The embodiment provides a technical scheme:
as shown in figures 1-4, the digestion device for the amino acid digestion experiment comprises an accommodating inner cylinder 2, a solid-liquid uniformly mixing mechanism and an outer sleeve. The solid-liquid blending mechanism comprises a fixing column 61 detachably connected with the lower end of the accommodating inner cylinder 2, a first elastic piece 62, a blending cylinder 1 and a storage mechanism for storing reagents. The bottom of a mixing section of thick bamboo 1 is equipped with the through-hole that adaptation fixed column 61 passed, and the lateral wall of fixed column 61 is located to first elastic component 62 cover, and the one end of first elastic component 62 is fixed mutually with the bottom that holds inner tube 2, and the other end is fixed with 1 bottom of a mixing section of thick bamboo. The open end of outer sleeve is equipped with the start-stop mechanism of sealed gas or ventilation, holds inner tube 2 and solid-liquid mixing mechanism and all sets up in the inside of outer sleeve, and outer sleeve back to open end one side is equipped with the joint with the switch-on of nitrogen gas breather pipe, correspondingly, holds the lateral wall of inner tube 2 and is equipped with the nitrogen gas outflow hole 21 that supplies the nitrogen gas outflow to get into the outer sleeve, holds and leaves the clearance that supplies the nitrogen gas to pass through between inner tube 2 and the outer sleeve.
In the present invention, the experimenter may place the sample on the mixing drum 1 and inject the reagent into the storage mechanism. Because the fixed end head and the bottom end of the mixing cylinder 1 are arranged in a sliding way. So the experiment personnel accessible rocks the outer sleeve from top to bottom for mixing section of thick bamboo 1 moves in vertical direction, and with store the liquid of flowing out in the mechanism and carry out the mixing. When the sample and the reagent are mixed uniformly, the mixture flows into the bottom of the accommodating inner barrel 2 to wait for digestion reaction. Since the digestion reaction of amino acid is anaerobic, the experimenter can introduce nitrogen through the joint and discharge the nitrogen from the nitrogen outlet hole 21 into the outer sleeve along the gap between the inner containing cylinder and the outer sleeve. And finally, discharging the nitrogen through the opening and closing mechanism, and performing a microwave digestion experiment when the air in the outer sleeve is exhausted. By using the digestion device provided by the invention, an experimenter can fully mix the sample and the reagent, so that the digestion effect is better.
As shown in fig. 3, the end of the fixing post 61 facing away from the inner receptacle 2 is provided with a fixing head 64. The storage mechanism comprises a connecting column 65, a connecting rod 66, a hollow positioning sleeve 67 with two open ends and a liquid storage cylinder 33. One end of the connecting column 65 is detachably connected with the fixed end 64, the other end of the connecting column is fixed with the connecting rod 66, and one end of the connecting rod 66, which is back to the connecting column 65, is fixed with the outer side wall of the positioning sleeve 67. The outer sleeve comprises a top part 37, the liquid storage cylinder 33 is fixed with the inward side of the top part 37, and the liquid storage cylinder 33 slides in the hollow part of the positioning sleeve 67. The storage mechanism also comprises an opening and closing piece which is used for controlling the reagent to flow out along with the up-and-down shaking of the blending cylinder 1.
As shown in fig. 4, the opening and closing member includes a positioning plate 68, a push rod 69, a second elastic member 35, and a sealing disk 34, which are disposed at an end of the positioning sleeve 67 facing away from the reservoir 33. The middle part of the positioning disc 68 is fixed with the mandril 69, and the positioning disc 68 is provided with a liquid outlet groove for the reagent to flow out. Correspondingly, a limiting ring is arranged on one side of the liquid storage cylinder 33 facing the ejector rod 69, a second elastic part 35 is fixedly connected above the limiting ring, one end, back to the limiting ring, of the second elastic part 35 is fixed with the sealing disc 34, and the diameter of the sealing disc 34 is smaller than that of the liquid storage cylinder 33. When the experimenter shakes up and down, the blending cylinder 1 moves in the vertical direction. At the same time, the positioning sleeve 67 fixed to the connection post 65 is also moved in the vertical direction. When the ejector rod 69 ejects the sealing disc 34 upwards, the reagent in the liquid storage barrel 33 can flow out along with the gap between the sealing disc 34 and the inner side wall of the liquid storage barrel 33, and finally flows out from the liquid outlet groove to be uniformly mixed with the sample.
As shown in fig. 4, one end of the liquid storage cylinder 33 opposite to the sealing disc 34 is provided with a liquid injection through hole, and the liquid injection through hole is provided with a plug 31.
As shown in fig. 2, the outer sleeve further includes a side portion 36 and a connecting portion 4 in a column shape, one end of the side portion 36 is fixed to the top portion 37, the other end is detachably connected to the connecting portion 4, and a sealing plate 42 is fixedly disposed inside the connecting portion 4. The opening and closing mechanism comprises a connecting rod 43 and a sliding sleeve ring 44, wherein the connecting rod 43 and the sliding sleeve ring 44 are fixed to the lower end of the sealing plate 42, and the sliding sleeve ring 44 is sleeved on the outer side wall of the connecting rod 43 in a sliding mode. The sealing plate 42 is provided with a nitrogen gas outflow groove, and a gas flow sealing plate 41 is rotatably connected to the wall of the nitrogen gas outflow groove on one side. The upper end of the slip collar 44 is provided with a jack-up rod 440 that jacks up the flow seal plate 41 to leave a gap through which nitrogen gas passes. The airflow sealing plate 41 and the sealing plate 42 are connected in a reversible manner (only schematically shown in the figure) and can only be turned towards the inner side of the connecting part 4. When the experimenter drives the jacking rod 440 on the sliding collar 44 to jack the gas flow sealing plate 41 upwards, the nitrogen gas can be exhausted.
As shown in fig. 2, the present embodiment further includes a detection mechanism for detecting whether the air in the inner area of the outer sleeve is exhausted, the detection mechanism includes an air cylinder 5 detachably connected to the connection portion 4, a fixing plate 51 is disposed at an opening of the air cylinder 5, a nitrogen through groove 52 is disposed on the fixing plate 51, the fixing plate 51 is provided with a through hole 53 adapted to the connection rod 43 to pass through, and the air cylinder 5 is made of a transparent material.
In the present embodiment, an indication slide plate is slidably provided inside the air cylinder 5. The experimenter can judge whether the air in the outer sleeve is completely discharged or not by observing the indicating sliding disc in the air storage cylinder 5. In principle, it is assumed that the air in the inner region of the outer sleeve is exhausted as long as the volume of the exhausted air corresponds to the volume of the inner region of the outer sleeve.
As shown in FIG. 2, the junction of the side portion 36 and the top portion 37 is provided with an arc-shaped fixing portion 370 adapted to receive the inner cylinder 2.
In this embodiment, the kneading cylinder 1 is provided with a plurality of circular holes through which the mixture of the sample and the reagent passes.
As shown in fig. 2 (in the state of fig. 2, the fixing piece 63 is not rotated into the insertion groove 38 for fixing), one end of the fixing post 61 opposite to the fixing end 64 is provided with the fixing piece 63, and correspondingly, the bottom of the accommodating inner cylinder 2 is provided with the insertion groove 38 which is matched with the fixing piece 63 and is inserted after rotating.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the invention can be made, and equivalents and modifications of some features of the invention can be made without departing from the spirit and scope of the invention.
Claims (9)
1. A digestion device for an amino acid digestion experiment is characterized by comprising an accommodating inner cylinder (2), a solid-liquid mixing mechanism and an outer sleeve;
the solid-liquid blending mechanism comprises a fixing column (61) detachably connected with the lower end of the accommodating inner cylinder (2), a first elastic piece (62), a blending cylinder (1) and a storage mechanism for storing reagents;
the bottom end of the blending cylinder (1) is provided with a through hole through which the adaptive fixing column (61) penetrates, the outer side wall of the fixing column (61) is sleeved with the first elastic piece (62), one end of the first elastic piece (62) is fixed with the bottom end of the containing inner cylinder (2), and the other end of the first elastic piece is fixed with the bottom end of the blending cylinder (1);
the opening end of the outer sleeve is provided with a sealing gas or ventilating opening and closing mechanism, the accommodating inner cylinder (2) and the solid-liquid uniformly mixing mechanism are both arranged inside the outer sleeve, one side of the outer sleeve, which is opposite to the opening end, is provided with a connector communicated with the nitrogen gas ventilating pipe, correspondingly, the side wall of the accommodating inner cylinder (2) is provided with a nitrogen gas flow outlet (21) for nitrogen gas to flow out and enter the outer sleeve, and a gap for nitrogen gas to pass through is reserved between the accommodating inner cylinder (2) and the outer sleeve;
one end of the fixing column (61), which is back to the accommodating inner cylinder (2), is provided with a fixing end head (64);
the storage mechanism comprises a connecting column (65), a connecting rod (66), a hollow positioning sleeve (67) with two open ends and a liquid storage cylinder (33);
one end of the connecting column (65) is detachably connected with the fixed end (64), the other end of the connecting column is fixed with the connecting rod (66), and one end of the connecting rod (66) back to the connecting column (65) is fixed with the outer side wall of the positioning sleeve (67);
the outer sleeve comprises a top part (37), the liquid storage cylinder (33) is fixed with the inward side of the top part (37), and the liquid storage cylinder (33) slides in the hollow part of the positioning sleeve (67);
the storage mechanism also comprises a starting and closing piece which is rocked up and down along with the blending cylinder (1) and is used for controlling the reagent to flow out.
2. The digestion device for the amino acid digestion experiment according to the claim 1, wherein the opening and closing piece comprises a positioning disc (68), a mandril (69), a second elastic piece (35) and a sealing disc (34) which are arranged at one end of a positioning sleeve (67) which is opposite to the liquid storage cylinder (33);
the middle part of the positioning disc (68) is fixed with the ejector rod (69), and the positioning disc (68) is provided with a liquid outlet groove for the reagent to flow out;
correspondingly, liquid storage barrel (33) are equipped with the spacing ring towards ejector pin (69) one side, the top fixedly connected with second elastic component (35) of spacing ring, the one end and the sealed disc (34) of second elastic component (35) spacing ring dorsad are fixed mutually, the diameter of sealed disc (34) is less than liquid storage barrel (33).
3. The digestion device for the amino acid digestion experiment according to the claim 2, characterized in that the end of the liquid storage cylinder (33) opposite to the sealing disc (34) is provided with a liquid injection through hole, and the liquid injection through hole is provided with a plug (31).
4. The digestion apparatus for amino acid digestion experiments according to claim 1, wherein the outer sleeve further comprises a side part (36) and a connecting part (4) in a column shape, one end of the side part (36) is fixed with the top part (37), the other end is detachably connected with the connecting part (4), and a sealing plate (42) is fixedly arranged on the inner side of the connecting part (4);
the opening and closing mechanism comprises a connecting rod (43) and a sliding sleeve ring (44), wherein the connecting rod (43) is fixed with the lower end of the sealing plate (42), and the sliding sleeve ring (44) is sleeved on the outer side wall of the connecting rod (43) in a sliding manner;
the sealing plate (42) is provided with a nitrogen gas outflow groove, and the wall of the nitrogen gas outflow groove is connected with an airflow sealing plate (41) in a manner of rotating on one side;
the upper end of the sliding sleeve ring (44) is provided with a jacking rod (440) which jacks up the airflow sealing plate (41) to leave a gap for nitrogen to pass through.
5. The digestion device for the amino acid digestion experiment according to claim 1, further comprising a detection mechanism for detecting whether the air in the inner area of the outer sleeve is exhausted, wherein the detection mechanism comprises an air storage cylinder (5) detachably connected with the connecting part (4), a fixing plate (51) is arranged at the opening part of the air storage cylinder (5), a nitrogen through groove (52) is arranged on the fixing plate (51), the fixing plate (51) is provided with a through hole (53) for the adaptive connecting rod (43) to pass through, and the air storage cylinder (5) is made of a transparent material.
6. The digestion device for amino acid digestion experiments according to claim 5, wherein the inside of the air cylinder (5) is slidably provided with an indicating slide plate.
7. The digestion apparatus for amino acid digestion experiments as claimed in claim 4, wherein the junction of the side portion (36) and the top portion (37) is provided with an arc-shaped fixing portion (370) adapted to accommodate the insertion of the inner barrel (2).
8. The digestion device for the amino acid digestion experiment according to the claim 1, characterized in that the mixing drum (1) is provided with a plurality of round holes for the mixture of the sample and the reagent to pass through.
9. The digestion device for amino acid digestion experiments as claimed in claim 1, characterized in that the end of the fixed column (61) opposite to the fixed end head (64) is provided with a fixed plate (63), and correspondingly, the bottom of the containing inner cylinder (2) is provided with a caulking groove (38) which is matched with the fixed plate (63) and is embedded after the fixed plate (63) rotates.
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