CN110208076A - A kind of digestion instrument for amino acid resolution experiment - Google Patents

A kind of digestion instrument for amino acid resolution experiment Download PDF

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Publication number
CN110208076A
CN110208076A CN201910515038.5A CN201910515038A CN110208076A CN 110208076 A CN110208076 A CN 110208076A CN 201910515038 A CN201910515038 A CN 201910515038A CN 110208076 A CN110208076 A CN 110208076A
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China
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cylinder
fixed
outer sleeve
inner cylinder
nitrogen
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CN201910515038.5A
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CN110208076B (en
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李樱红
徐梦
徐涛
龚青
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ZHEJIANG INSTITUTE FOR FOOD AND DRUG CONTROL
Zhejiang Special Equipment Inspection and Research Institute
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ZHEJIANG INSTITUTE FOR FOOD AND DRUG CONTROL
Zhejiang Special Equipment Inspection and Research Institute
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/44Sample treatment involving radiation, e.g. heat

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention discloses a kind of digestion instruments for amino acid resolution experiment, and in particular to a kind of laboratory apparatus, including accommodate inner cylinder, solid-liquid mixing mechanism and outer sleeve;Solid-liquid mixing mechanism includes fixed column, the first elastic component, mixes cylinder and stocking mechanism;The bottom end for mixing cylinder is equipped with the through-hole that adaptation fixed column passes through, and the first elastic component is sheathed on the lateral wall of fixed column, and one end of the first elastic component is mutually fixed with the bottom end for accommodating inner cylinder, and the other end is mutually fixed with cylinder bottom is mixed;The open end of outer sleeve is equipped with the mechanism for opening/closing of sealing gas or ventilation, it accommodates inner cylinder and solid-liquid mixing mechanism is all set in the inside of outer sleeve, outer sleeve is equipped with the connector connected with nitrogen snorkel backwards to open end side, correspondingly, the side wall for accommodating inner cylinder is equipped with the nitrogen tap hole for entering outer sleeve for nitrogen outflow, and there are the gaps passed through for nitrogen between receiving inner cylinder and outer sleeve.The present invention has the advantages that experimenter can be facilitated to realize homogeneous by concussion.

Description

A kind of digestion instrument for amino acid resolution experiment
Technical field
The present invention relates to a kind of laboratory apparatus, specially a kind of digestion instrument for amino acid resolution experiment.
Background technique
In the measurement to the element in sample, need to carry out resolution processing to sample.The effect of resolution processing is to destroy Organic matter and dissolved particles object, and the element oxide to be measured of various valence states at single high-valence state or is converted into easily decomposes Inorganic compound.
It wherein, in the experimentation of resolution amino acid, is carried out in oxygen-free environment.Experimenter's common practice It is to be added to sample and reagent in tiny glass tube, and be passed through enough nitrogen air is discharged, then into glass tube It is sealed using alcohol blast burner.However, in above-mentioned experimentation, when sample powder is added to glass tube, sample powder Intermediate position be often difficult to come into full contact with reagent, and then will affect resolution effect.
Summary of the invention
The present invention provides it is a kind of for amino acid resolution experiment digestion instrument, the digestion instrument have can facilitate experiment The advantages of personnel realize homogeneous by concussion.
To achieve the above object, the invention provides the following technical scheme:
A kind of digestion instrument for amino acid resolution experiment, including accommodate inner cylinder, solid-liquid mixing mechanism and outer sleeve; The solid-liquid mixing mechanism includes the fixed column being detachably connected with receiving inner cylinder lower end, the first elastic component, mixes cylinder and use In the stocking mechanism for depositing installed reagents;The bottom end for mixing cylinder is equipped with the through-hole that adaptation fixed column passes through, first elastic component It is sheathed on the lateral wall of fixed column, one end of first elastic component is mutually fixed with the bottom end for accommodating inner cylinder, the other end and mixing Cylinder bottom is mutually fixed;The open end of the outer sleeve is equipped with the mechanism for opening/closing of sealing gas or ventilation, the receiving inner cylinder and Solid-liquid mixing mechanism is all set in the inside of outer sleeve, and the outer sleeve is equipped with backwards to open end side and connects with nitrogen snorkel Connector, correspondingly, it is described accommodate inner cylinder side wall be equipped with for nitrogen outflow enter outer sleeve nitrogen tap hole, accommodate inner cylinder There are the gaps passed through for nitrogen between outer sleeve.
Preferably, the fixed column is equipped with fixed end backwards to the one end for accommodating inner cylinder;The stocking mechanism includes connecting Connect column, connecting rod, both ends open and hollow positioning sleeve and liquid storage cylinder;One end of the connecting column and fixed end are detachable Connection, the other end are mutually fixed with connecting rod, and the connecting rod is mutually fixed backwards to one end of connecting column and the lateral wall of positioning sleeve;Institute Stating outer sleeve includes top, and the liquid storage cylinder and the side inwardly at top are mutually fixed, and the liquid storage cylinder is in the hollow portion of positioning sleeve Position sliding;The stocking mechanism further includes the opening and closing element for teetertottering with cylinder is mixed and being used to control reagent outflow.
Preferably, the opening and closing element includes that positioning sleeve is arranged in backwards to the positioning disk of liquid storage cylinder one end, mandril, the second bullet Property part and sealed disc;The middle part of the positioning disk is mutually fixed with mandril, and the positioning disk, which is equipped with, goes out liquid for what reagent flowed out Slot;Correspondingly, the liquid storage cylinder is equipped with stop collar towards mandril side, and the second elasticity is fixedly connected with above the stop collar Part, second elastic component are mutually fixed backwards to one end of stop collar with sealed disc, and the diameter of the sealed disc is less than liquid storage Cylinder.
Preferably, the liquid storage cylinder is equipped with fluid injection through-hole backwards to one end of sealed disc, the fluid injection through-hole is equipped Plug.
Preferably, the outer sleeve further includes side and the interconnecting piece being in the form of a column, one end and top of the side Mutually fixed, the other end is detachably connected with interconnecting piece, is fixed with sealing plate on the inside of the interconnecting piece;The mechanism for opening/closing packet Include the connecting rod mutually fixed with sealing plate lower end and slip collar, the slip collar is slidably located in the lateral wall of connecting rod On;The sealing plate is equipped with nitrogen spout, and the cell wall of the nitrogen spout unilateral can rotationally be connected with air current sealed plate; The upper end of the slip collar is equipped with and jacks up air current sealed plate upwards to reserve the jack-up bar in the gap passed through for nitrogen.
Preferably, the digestion instrument further includes the detection machine whether drained for detecting the air of outer sleeve interior zone Structure, the testing agency include the gas receiver being detachably connected with interconnecting piece, and the opening position of the gas receiver is equipped with fixed plate, The fixed plate is equipped with nitrogen through slot, and the fixed plate is equipped with the perforation that adaptation connecting rod passes through, and the gas receiver is by transparent Material is made.
Preferably, the internal slide of the gas receiver is equipped with instruction sliding plate.
Preferably, the side is equipped with the junction at top is adapted to the arc fixed part for accommodating inner cylinder insertion.
Preferably, the cylinder that mixes is equipped with the circular hole that multiple mixtures for sample and reagent pass through.
Preferably, the fixed column is equipped with fixinig plate backwards to one end of fixed end, correspondingly, the receiving inner cylinder Bottom is equipped with the caulking groove being embedded in after adaptation fixinig plate rotation.
The invention has the benefit that
In the present invention, sample can be placed on by experimenter mixes on cylinder, and reagent is injected in stocking mechanism.Due to Fixed end and the bottom end for mixing cylinder are sliding settings.Therefore experimenter can be by the outer sleeve that fluctuates, so that mixing cylinder It moves, and is mixed with the liquid flowed out in stocking mechanism in the vertical direction.After sample and reagent mix, it can be flowed into The bottom of inner cylinder is accommodated, to wait for resolution reaction.Since amino acid resolution reaction is anaerobism, experimenter can be by nitrogen It is passed through by connector, and is discharged by nitrogen tap hole, along the gap accommodated between inner cylinder and outer sleeve, enter outer sleeve In.Nitrogen is discharged eventually by mechanism for opening/closing, when the air inside outer sleeve is drained, can carry out micro-wave digestion experiment. Experimenter can adequately be mixed sample and reagent, by using digestion instrument provided by the invention so that disappearing Solve better effect.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of the digestion instrument for amino acid resolution experiment in the present embodiment;
Fig. 2 is a kind of the schematic diagram of the section structure of the digestion instrument for amino acid resolution experiment in the present embodiment;
Fig. 3 is the portion the A enlarged drawing in Fig. 2;
Fig. 4 is the portion the B enlarged drawing in Fig. 2.
In figure: 1, mixing cylinder, 2, receiving inner cylinder, 21, nitrogen tap hole, 31, plug, 33, liquid storage cylinder, 34, sealed disc, 35, the second elastic component, 36, side, 37, top, 370, arc fixed part, 38, caulking groove, 4, interconnecting piece, 41, air current sealed plate, 42, sealing plate, 43, connecting rod, 44, slip collar, 440, jack-up bar, 5, gas receiver, 51, fixed plate, 52, nitrogen through slot, 53, Perforation, 61, fixed column, the 62, first elastic component, 64, fixed end, 65, connecting column, 66, connecting rod, 67, positioning sleeve, 68, fixed Position disk, 69, mandril.
Specific embodiment
The present embodiment provides a kind of technical solutions:
As shown in figures 1-4, a kind of digestion instrument for amino acid resolution experiment, including accommodate inner cylinder 2, solid-liquid mixing Mechanism and outer sleeve.Solid-liquid mixing mechanism includes and accommodates fixed column 61, the first elastic component that 2 lower end of inner cylinder is detachably connected 62, cylinder 1 and the stocking mechanism for depositing installed reagents are mixed.The bottom end for mixing cylinder 1 is equipped with the through-hole that adaptation fixed column 61 passes through, First elastic component 62 is sheathed on the lateral wall of fixed column 61, and one end of the first elastic component 62 is mutually fixed with the bottom end for accommodating inner cylinder 2, The other end is mutually fixed with 1 bottom of cylinder is mixed.The open end of outer sleeve is equipped with the mechanism for opening/closing of sealing gas or ventilation, accommodates inner cylinder 2 and solid-liquid mixing mechanism be all set in the inside of outer sleeve, outer sleeve is equipped with backwards to open end side and connects with nitrogen snorkel Logical connector 31, correspondingly, the side wall for accommodating inner cylinder 2 are equipped with the nitrogen tap hole 21 for entering outer sleeve for nitrogen outflow, accommodate There are the gaps passed through for nitrogen between inner cylinder 2 and outer sleeve.
In the present invention, sample can be placed on by experimenter mixes on cylinder 1, and reagent is injected in stocking mechanism.By It is that sliding is arranged in the bottom end of fixed end and mixing cylinder 1.Therefore experimenter can be by the outer sleeve that fluctuates, so that mixing Cylinder 1 moves in the vertical direction, and is mixed with the liquid flowed out in stocking mechanism.After sample and reagent mix, it can flow Enter to the bottom for accommodating inner cylinder 2, to wait for resolution reaction.Since amino acid resolution reaction is anaerobism, experimenter can Nitrogen is passed through by connector 31, and is discharged by nitrogen tap hole 21, along the gap accommodated between inner cylinder and outer sleeve, is entered Into outer sleeve.Nitrogen is discharged eventually by mechanism for opening/closing, when the air inside outer sleeve is drained, can carry out microwave and disappear Solution experiment.Experimenter can adequately be mixed sample and reagent by using digestion instrument provided by the invention, thus So that resolution better effect.
As shown in figure 3, fixed column 61 is equipped with fixed end 64 backwards to the one end for accommodating inner cylinder 2.Stocking mechanism includes connection Column 65, connecting rod 66, both ends open and hollow positioning sleeve 67 and liquid storage cylinder 33.One end of connecting column 65 and fixed end 64 It is detachably connected, the other end is fixed with 66 phase of connecting rod, and connecting rod 66 is backwards to one end of connecting column 65 and the outside of positioning sleeve 67 Wall is mutually fixed.Outer sleeve includes top 37, and liquid storage cylinder 33 and the side inwardly at top 37 are mutually fixed, and liquid storage cylinder 33 is in positioning sleeve 67 Hollow part sliding.Stocking mechanism further includes the opening and closing element for teetertottering with cylinder 1 is mixed and being used to control reagent outflow.
As shown in figure 4, opening and closing element include be arranged in positioning sleeve 67 backwards to the positioning disk 68 of 33 one end of liquid storage cylinder, mandril 69, Second elastic component 35 and sealed disc 34.The middle part of positioning disk 68 is fixed with 69 phase of mandril, and positioning disk 68 is equipped with for reagent flow Liquid outlet groove out.Correspondingly, liquid storage cylinder 33 is equipped with stop collar towards 69 side of mandril, is fixedly connected with second above stop collar Elastic component 35, the second elastic component 35 are fixed backwards to one end of stop collar with 34 phase of sealed disc, and the diameter of sealed disc 34 is less than Liquid storage cylinder 33.When experimenter teetertotters, mixes cylinder 1 and move in the vertical direction.Meanwhile being fixed with 65 phase of connecting column Positioning sleeve 67 also moves in the vertical direction therewith.When mandril 69 jacks up sealed disc 34 upwards, the reagent of liquid storage cylinder 33 It can be flowed out with the gap between 33 inner sidewall of sealed disc 34 and liquid storage cylinder, finally outflow and sample blending from liquid outlet groove.
As shown in figure 4, liquid storage cylinder 33 is equipped with fluid injection through-hole backwards to one end of sealed disc 34, fluid injection through-hole is equipped with plug 31。
As shown in Fig. 2, outer sleeve further includes side 36 and the interconnecting piece being in the form of a column 4, one end and top 37 of side 36 Mutually fixed, the other end is detachably connected with interconnecting piece 4, and the inside of interconnecting piece 4 is fixed with sealing plate 42.Mechanism for opening/closing include with The connecting rod 43 and slip collar 44 that 42 lower end of sealing plate is mutually fixed, slip collar 44 are slidably located in the outside of connecting rod 43 On wall.Sealing plate 42 is equipped with nitrogen spout, and the cell wall of nitrogen spout unilateral can rotationally be connected with air current sealed plate 41.It is sliding The upper end of dynamic lantern ring 44, which is equipped with, jacks up air current sealed plate 41 upwards to reserve the jack-up bar 440 in the gap passed through for nitrogen.Gas Stream sealing plate 41 and sealing plate 42 are turnable connection (only illustrating in figure), and can only turning inside out towards interconnecting piece 4. When experimenter drives the jack-up bar 440 on slip collar 44 to jack up air current sealed plate 41 upwards, nitrogen can be discharged.
As shown in Fig. 2, the present embodiment further includes the detection machine whether drained for detecting the air of outer sleeve interior zone Structure, testing agency include the gas receiver 5 being detachably connected with interconnecting piece 4, and the opening position of gas receiver 5 is equipped with fixed plate 51, Gu Fixed board 51 is equipped with nitrogen through slot 52, and fixed plate 51 is equipped with the perforation 53 that adaptation connecting rod 43 passes through, and gas receiver 5 is by transparent material Matter is made.
In the present embodiment, the internal slide of gas receiver 5 is equipped with instruction sliding plate.Experimenter can be by observing gas receiver 5 Interior instruction sliding plate judges whether the air in outer sleeve is all discharged.As long as in the volume of air and outer sleeve that are discharged in principle When the volume in portion region is consistent, i.e. the air of expression outer sleeve interior zone has drained.
As shown in Fig. 2, side 36 is equipped with the arc fixed part 370 for being adapted to and accommodating inner cylinder 2 and being inserted into the junction at top 37.
In the present embodiment, it mixes cylinder 1 and is equipped with the circular hole that multiple mixtures for sample and reagent pass through.
(state in Fig. 2 is that fixinig plate 63 is not transferred to fixation in caulking groove 38) as shown in Figure 2, fixed column 61 is backwards to fixed One end of end 64 is equipped with fixinig plate 63, and correspondingly, the bottom for accommodating inner cylinder 2 is embedding equipped with being embedded in after the adaptation rotation of fixinig plate 63 Slot 38.
Although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art, It is still possible to modify the technical solutions described in the foregoing embodiments, or part of technical characteristic is carried out etc. With replacement, all within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in this Within the protection scope of invention.

Claims (10)

1. a kind of digestion instrument for amino acid resolution experiment, which is characterized in that including accommodating inner cylinder (2), solid-liquid mixing machine Structure and outer sleeve;
The solid-liquid mixing mechanism includes and accommodates fixed column (61), the first elastic component that inner cylinder (2) lower end is detachably connected (62), cylinder (1) and the stocking mechanism for depositing installed reagents are mixed;
The bottom end for mixing cylinder (1) is equipped with the through-hole that adaptation fixed column (61) passes through, and first elastic component (62) is sheathed on The lateral wall of fixed column (61), one end of first elastic component (62) and the bottom end for accommodating inner cylinder (2) are mutually fixed, the other end with Cylinder (1) bottom is mixed mutually to fix;
The open end of the outer sleeve is equipped with the mechanism for opening/closing of sealing gas or ventilation, and the receiving inner cylinder (2) and solid-liquid are mixed Even mechanism is all set in the inside of outer sleeve, and the outer sleeve is equipped with the connector connected with nitrogen snorkel backwards to open end side (31), correspondingly, the side wall for accommodating inner cylinder (2) is equipped with the nitrogen tap hole (21) for entering outer sleeve for nitrogen outflow, holds Receive between inner cylinder (2) and outer sleeve that there are the gaps passed through for nitrogen.
2. a kind of digestion instrument for amino acid resolution experiment according to claim 1, which is characterized in that the fixation Column (61) is equipped with fixed end (64) backwards to the one end for accommodating inner cylinder (2);
The stocking mechanism includes connecting column (65), connecting rod (66), both ends open and hollow positioning sleeve (67) and liquid storage Cylinder (33);
One end of the connecting column (65) is detachably connected with fixed end (64), and the other end is mutually fixed with connecting rod (66), institute Connecting rod (66) are stated mutually to fix backwards to the one end of connecting column (65) and the lateral wall of positioning sleeve (67);
The outer sleeve includes top (37), and the liquid storage cylinder (33) and the side inwardly of top (37) are mutually fixed, the liquid storage Cylinder (33) is slided in the hollow part of positioning sleeve (67);
The stocking mechanism further includes the opening and closing element for teetertottering with cylinder (1) is mixed and being used to control reagent outflow.
3. a kind of digestion instrument for amino acid resolution experiment according to claim 2, which is characterized in that the opening and closing Part includes positioning disk (68), mandril (69), second elastic component (35) of the setting in positioning sleeve (67) backwards to liquid storage cylinder (33) one end And sealed disc (34);
The middle part of the positioning disk (68) is mutually fixed with mandril (69), and the positioning disk (68), which is equipped with, goes out liquid for what reagent flowed out Slot;
Correspondingly, the liquid storage cylinder (33) is equipped with stop collar towards mandril (69) side, is fixedly connected above the stop collar Have the second elastic component (35), second elastic component (35) is mutually fixed backwards to one end of stop collar with sealed disc (34), described The diameter of sealed disc (34) is less than liquid storage cylinder (33).
4. a kind of digestion instrument for amino acid resolution experiment according to claim 3, which is characterized in that the liquid storage Cylinder (33) is equipped with fluid injection through-hole backwards to the one end of sealed disc (34), and the fluid injection through-hole is equipped with plug (31).
5. a kind of digestion instrument for amino acid resolution experiment according to claim 1, which is characterized in that the housing Cylinder further includes side (36) and the interconnecting piece (4) being in the form of a column, and one end and top (37) of the side (36) are mutually fixed, another End is detachably connected with interconnecting piece (4), is fixed with sealing plate (42) on the inside of the interconnecting piece (4);
The mechanism for opening/closing includes the connecting rod (43) mutually fixed with sealing plate (42) lower end and slip collar (44), the cunning Dynamic lantern ring (44) are slidably located on the lateral wall of connecting rod (43);
The sealing plate (42) is equipped with nitrogen spout, and it is close that the cell wall of the nitrogen spout unilateral can rotationally be connected with air-flow Sealing plate (41);
The upper end of the slip collar (44) is equipped with jacks up air current sealed plate (41) to reserve the gap passed through for nitrogen upwards Jack-up bar (440).
6. a kind of digestion instrument for amino acid resolution experiment according to claim 1, which is characterized in that further include using In the testing agency whether air of detection outer sleeve interior zone drains, the testing agency includes removable with interconnecting piece (4) The gas receiver (5) of connection is unloaded, the opening position of the gas receiver (5) is equipped with fixed plate (51), and the fixed plate (51) is equipped with Nitrogen through slot (52), the fixed plate (51) are equipped with the perforation (53) that adaptation connecting rod (43) passes through, and the gas receiver (5) is by saturating Bright material is made.
7. a kind of digestion instrument for amino acid resolution experiment according to claim 1, which is characterized in that the gas storage The internal slide of cylinder (5) is equipped with instruction sliding plate.
8. a kind of digestion instrument for amino acid resolution experiment according to claim 1, which is characterized in that the side (36) it is equipped with the junction of top (37) and is adapted to the arc fixed part (370) for accommodating inner cylinder (2) insertion.
9. a kind of digestion instrument for amino acid resolution experiment according to claim 1, which is characterized in that the mixing Cylinder (1) is equipped with the circular hole that multiple mixtures for sample and reagent pass through.
10. a kind of digestion instrument for amino acid resolution experiment according to claim 1, which is characterized in that described solid Fixed column (61) is equipped with fixinig plate (63) backwards to the one end of fixed end (64), and correspondingly, the bottom for accommodating inner cylinder (2) is equipped with The caulking groove (38) being embedded in after adaptation fixinig plate (63) rotation.
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