CN110199869A - A kind of horse bubble melon modification method - Google Patents
A kind of horse bubble melon modification method Download PDFInfo
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- CN110199869A CN110199869A CN201910535876.9A CN201910535876A CN110199869A CN 110199869 A CN110199869 A CN 110199869A CN 201910535876 A CN201910535876 A CN 201910535876A CN 110199869 A CN110199869 A CN 110199869A
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- melon
- seed
- pollination
- horse
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Abstract
A kind of horse bubble melon modification method, includes the following steps: that (1) hybrid parent selects；(2) selection of melon section is sat；(3) pollination time selects；(4) artificial pollination；(5) it is harvested after step 4 hybridization melon is mature, obtains full seed, second year plantation；(6) gained seed plants a generation after planting step 5 kind, is selfed a generation using the method for selfing；(7) seed for harvesting step 6, a plantation selfing generation, continuously plants 2-3 generation for selfed seed offspring；(8) step 7 is obtained and stablizes the plantation of single plant progeny seed, selfing, harvest seed；(9) step 8 is obtained into seed plantation, observes production performance, determines new varieties, declare cultivar registration.Melon volume becomes larger horse bubble after improvement, pulp thickens, sugariness increases, fruit type becomes ellipse, solves wild horse poor, the less problem of edible part of steeping melon edibility.
Description
Technical field
The present invention relates to field of plant variety breeding technology more particularly to a kind of horse to steep melon modification method.
Background technique
Horse steeps melon, and Curcurbitaceae, cucumber plants, nickname horse bezoar, small wild melon, pony bubble, horse bubble melon (mutation) is in Henan agriculture
Village is also referred to as horse bezoar egg.It does not often eat, watches, annual herb, source area Africa entangles and overgrows, entangling every section has one
Tendril, Ye Youbing, wedge shaped or heart-shaped, blade face is rougher, there is seta.7, August is bloomed, Hua Huangse, monoecism color, flower
Hat is split with 3-5, the long ellipse circular of ovary, style sleeve length, and 3 pieces of column cap.Melon varies, maximum as goose egg, the smallest as knob
Button.Melon taste has perfume (or spice) to have sweet tea, has acid to have hardship, and melon skin color has green, flower, white to have with green item.Seed is faint yellow, flat, long
Ellipse circular, surface is smooth, kernel white, flowering fruit bearing stage summer.There are a little cultivation in China, various regions, north and south, are commonly wild.Horse steeps melon
A kind of wild plant that Northern Area of Huaihe River is generally grown, the past it as weeds, hoe lest not to the utmost, currently, discovery Ma Pao
Melon is that one object of one kind can be mostly used, the higher wild plant of economic value can squeeze containing a large amount of oil especially in seed
Oil is a kind of good, extremely promising oil crops.Horse steep melon be it is wild melon, document shows horse bubble melon and cucurbit
Section's muskmelon platymiscium is closer to, it is circle in appearance, also close to muskmelon.Germicidal efficacy, horse, which steeps melon, has female series result
Characteristic.Column cap leakage, pollination are easy, theoretically can be with Cucumis plant hybridization.Because it is more that horse steeps melon seed, can extract oil,
But edibility is poor, edible part is less, and the muskmelon platymiscium that such as improve optional meat thickness is preferable, and the current country rarely has Ma Pao
The case that hybridizes of melon and muskmelon, therefore be with a wide range of applications for the improved application of horse bubble melon.
Summary of the invention
The purpose of the present invention is to provide a kind of horses to steep melon modification method, to solve the above technical problems.
The present invention using following technical scheme in order to solve the above technical problems, realized:
A kind of horse bubble melon modification method, includes the following steps:
(1) hybrid parent selects: wild horse bubble melon is cooked female parent；Cultivation muskmelon is cooked male parent；
(2) sit the selection of melon section: horse steeps melon cultivation based on not pruning, and is advisable in 5-6 section seat melon；
(3) pollination time selects: artificial pollination when -10 when the selection morning 7, the pollination in same plot are complete in -10 days 7 days
At；
(4) artificial pollination: the muskmelon male flower that the selection same day opens removes petal, exposes stamen, stamen pollen is gently applied
It is put on horse bubble melon pistil stigma, 2-3 female flowers can be applied in every male flower；
(5) it is harvested after gained hybridization melon is mature after step 4 pollination, method removal impurity is washed with water, obtains full seed, the
It plants within 2 years, is control with wild horse bubble melon, the hereditary shape and knot melon characteristic of hybridization melon are recorded in observation；
(6) gained seed plants a generation after planting step 5 kind, is selfed a generation using the method for selfing, harvests seed, enter
Strain identifies garden, identifies strain disease resistance, result, heat resistance in strain identification garden, Superior line harvests seed, remaining is washed in a pan
It eliminates；
(7) seed for harvesting step 6, a plantation selfing generation, selfed seed offspring is continuously planted 2-3 generation, product are observed
Kind stability；
(8) step 7 is obtained and stablizes the plantation of single plant progeny seed, selfing, harvest seed；
(9) step 8 is obtained into seed plantation, observes production performance, determines new varieties, declare cultivar registration.
Preferably, stamen pollen can be collected into vessel when artificial pollination in the step (3), then uses banister brush
Pollen is dipped, pistil stigma is gently smeared.
Preferably, when step (3) artificial pollination, female flower is sprayed using the drop-proof agent of 10 mg/kgs.
Preferably, as continuous overcast and rainy in met with when step (3) artificial pollination, first 1 day can rained to female flower and male flower
Paper cap is put on, female flower still puts on paper cap after pollination, removes after 3 days；It such as shower, should pollinate within 2 hours after the rain, if pollination
It meets with and drenches with rain in 3 hours afterwards, then need to mend and award 1 time.
Preferably, the female flower after artificial pollination will hang up playing cards, indicate pollination date.
Preferably, the temperature of plantation is maintained at 15 DEG C or more.
Preferably, cultivation muskmelon male parent is Dongfangmi No.1 in the step (1).
The beneficial effects of the present invention are:
1, melon volume becomes larger the horse bubble after improveing, pulp thickens, sugariness increases, fruit type becomes ellipse, solves wild
Horse bubble melon edibility is poor, the less problem of edible part.
2, it is female chapiter and male flower pollen physiological activity most vigorous period when -10 when the morning 7, is artificial pollination at this time
Best Times；The pollination in same plot is completed in -10 days 7 days, and bear fruit phase and picking time of melon can in this way concentrated, convenient
Management.
3, as continuous overcast and rainy in met with when artificial pollination, paper cap can be put on to female flower and male flower raining first 1 day, it is female after pollination
Flower still puts on paper cap, removes after 3 days；It such as shower, should pollinate within 2 hours after the rain, if meeting with rain in 3 hours after pollination
Leaching is then needed to mend and be awarded 1 time, in this way it is possible to prevente effectively from rainwater influences normal pollination.
4, the female flower after artificial pollination will hang up playing cards, indicate pollination date, can not only prevent from repeating to pollinate in this way, and
Convenient for determining the muskmelon maturity period, with sharp timely collecting.
Specific embodiment
In order to be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, tie below
Specific embodiment is closed, the present invention is further explained, and however, the following embodiments are merely preferred embodiments of the present invention, and not all.
Based on the implementation example in the implementation mode, those skilled in the art's obtained other realities without making creative work
Example is applied, protection scope of the present invention is belonged to.Experimental method in following embodiments is unless otherwise specified conventional method,
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
A kind of horse bubble melon modification method, includes the following steps:
(1) hybrid parent selects: wild horse bubble melon is cooked female parent；Dongfangmi No.1 cultivation muskmelon is cooked male parent；
Maternal wild horse bubble melon is precocious, and the time of infertility 90 days, fruit development period 34 days；Growing way is prosperous by force, Flower node the 4th
Section, section of bearing fruit Section 6；Growth period crust light green color, it is golden yellow after mature；Spheroidal, meat are 0.2 centimetre thick；10 grams or so are weighed,
Soluble solid 1-2 degree, meat is fine and smooth, sweet mouthfeel.
Male parent is that Dongfangmi No.1 is improved variety resource, is commercially introduced；Appearance white, ellipse is precocious,
The time of infertility 93 days, fruit development period 35 days；Growing way is prosperous by force；Flower node Section 5, section of bearing fruit Section 6,1-1.5 lis of meat thickness
Rice, weighs 130 grams.
(2) sit the selection of melon section: horse steeps melon cultivation based on not pruning, and is advisable in 5-6 section seat melon；
(3) pollination time selects: artificial pollination when -10 when the selection morning 7, the pollination in same plot are complete in -10 days 7 days
At；
(4) artificial pollination: the muskmelon male flower that the selection same day opens removes petal, exposes stamen, stamen pollen is gently applied
It is put on horse bubble melon pistil stigma, 2-3 female flowers can be applied in every male flower；
(5) it is harvested after gained hybridization melon is mature after step 4 pollination, method removal impurity is washed with water, obtains full seed, the
It plants within 2 years, is control with wild horse bubble melon, the hereditary shape and knot melon characteristic of hybridization melon are recorded in observation；
(6) gained seed plants a generation after planting step 5 kind, is selfed a generation using the method for selfing, harvests seed, enter
Strain identifies garden, identifies strain disease resistance, result, heat resistance in strain identification garden, Superior line harvests seed, remaining is washed in a pan
It eliminates；
(7) seed for harvesting step 6, a plantation selfing generation, selfed seed offspring is continuously planted 2-3 generation, product are observed
Kind stability；
(8) step 7 is obtained and stablizes the plantation of single plant progeny seed, selfing, harvest seed；
(9) step 8 is obtained into seed plantation, observes production performance, determines new varieties Huang Ma Pao, declare cultivar registration.
In step (3) when artificial pollination, stamen pollen can be collected into vessel, then dip pollen with banister brush, gently
Dab smears pistil stigma.
When step (3) artificial pollination, female flower is sprayed using the drop-proof agent of 10 mg/kgs.
It is as continuous overcast and rainy in met with when step (3) artificial pollination, paper cap can be put on to female flower and male flower raining first 1 day, awarded
Female flower still puts on paper cap after powder, removes after 3 days；It such as shower, should pollinate within 2 hours after the rain, if meeting in 3 hours after pollination
Chance drenches with rain, then needs to mend and award 1 time.
Female flower after artificial pollination will hang up playing cards, indicate pollination date.
Yellow horse steeps breediness are as follows:
The kind is precocious, and the time of infertility 106 days, fruit development period 35 days, growing way was prosperous by force, Flower node Section 5, section of bearing fruit
Position Section 6；Crust yellow, 45 grams of mean fruit weight or so；Fruit type ellipse, averagely vertical 5.87 centimetres of diameter, 4.74 centimetres of transverse diameter,
Fruit type index 1.24；Meat is 0.73 centimetre thick, soluble solid 4-5 degree, and meat is fine and smooth, and mouthfeel is slightly sweet.
Suitable planting area:
This kind is suitable for planting in Anhui Province's North of Huai River spring, summer.
Cultural technigue:
1. mu is kept a full stand of seedings 3500 plants mu with 0.1 kilogram of seed；
2. March in spring plants, plantation in July summer；
3. cultivating naturally, cross of not pruning, nature result；
4. mu 30 kilograms of compound fertilizer of application；
5. 15 DEG C of suitable planting temperature or more.
The horse bubble melon of the present embodiment breeding is improved the breed better than in place of original kind are as follows:
1. weight becomes larger, by wild 10g or so, improve to weigh about 45g；
2. fruit type changes: by wild circle, becoming ellipse；
3. pulp increases: increasing to thick 0.73cm by wild thick 0.2cm；
4. sugariness increases: being increased to 4-5 degree by wild 1-2 degree.
Therefore improvement purpose has been reached, has steeped melon for improvement horse in next step and established application foundation.
The basic principles, main features and advantages of the present invention have been shown and described above.The technology of the industry
For personnel it should be appreciated that the present invention is not limited to the above embodiments, described in the above embodiment and specification is only the present invention
Preference, be not intended to limit the invention, without departing from the spirit and scope of the present invention, the present invention also has various
Changes and improvements, these changes and improvements all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by institute
Attached claims and its equivalent thereof.
Claims (7)
1. a kind of horse steeps melon modification method, which comprises the steps of:
(1) hybrid parent selects: wild horse bubble melon is cooked female parent；Cultivation muskmelon is cooked male parent；
(2) sit the selection of melon section: horse steeps melon cultivation based on not pruning, and is advisable in 5-6 section seat melon；
(3) pollination time selects: artificial pollination when -10 when the selection morning 7, the pollination in same plot are completed in -10 days 7 days；
(4) artificial pollination: the muskmelon male flower that the selection same day opens removes petal, exposes stamen, stamen pollen is gently applied to
Horse is steeped on melon pistil stigma, and 2-3 female flowers can be applied in every male flower；
(5) it is harvested after gained hybridization melon is mature after step 4 pollination, method removal impurity is washed with water, obtains full seed, second year
Plantation, is control with wild horse bubble melon, and the hereditary shape and knot melon characteristic of hybridization melon are recorded in observation；
(6) gained seed plants a generation after planting step 5 kind, is selfed a generation using the method for selfing, seed is harvested, into strain
It identifies garden, identifies strain disease resistance, result, heat resistance in strain identification garden, Superior line harvests seed, remaining is eliminated；
(7) seed for harvesting step 6, a plantation selfing generation, selfed seed offspring is continuously planted 2-3 generation, observation kind is steady
It is qualitative；
(8) step 7 is obtained and stablizes the plantation of single plant progeny seed, selfing, harvest seed；
(9) step 8 is obtained into seed plantation, observes production performance, determines new varieties, declare cultivar registration.
2. a kind of horse according to claim 1 steeps melon modification method, it is characterised in that: artificial pollination in the step (3)
When, stamen pollen can be collected into vessel, then dip pollen with banister brush, gently smear pistil stigma.
3. a kind of horse according to claim 1 steeps melon modification method, it is characterised in that: when step (3) artificial pollination,
Female flower is sprayed using the drop-proof agent of 10 mg/kgs.
4. a kind of horse according to claim 1 steeps melon modification method, it is characterised in that: when step (3) artificial pollination
Continuous wet weather is such as met with, puts on paper cap to female flower and male flower within first 1 day raining, female flower still puts on paper cap after pollination, takes after 3 days
Under；It such as shower, should pollinate within 2 hours, drench with rain if being met in 3 hours after pollination after the rain, need to mend and award 1 time.
5. a kind of horse according to claim 1 steeps melon modification method, it is characterised in that: the female flower after artificial pollination will be hung up
Playing cards indicate pollination date.
6. a kind of horse according to claim 1 steeps melon modification method, it is characterised in that: the temperature of plantation be maintained at 15 DEG C with
On.
7. a kind of horse according to claim 1 steeps melon modification method, it is characterised in that: cultivate muskmelon in the step (1)
Male parent is Dongfangmi No.1.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910535876.9A CN110199869A (en) | 2019-06-20 | 2019-06-20 | A kind of horse bubble melon modification method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910535876.9A CN110199869A (en) | 2019-06-20 | 2019-06-20 | A kind of horse bubble melon modification method |
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| CN110199869A true CN110199869A (en) | 2019-09-06 |
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| CN201910535876.9A Withdrawn CN110199869A (en) | 2019-06-20 | 2019-06-20 | A kind of horse bubble melon modification method |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2274974A1 (en) * | 2005-12-21 | 2011-01-19 | Monsanto Invest N.V. | Closterovirus-resistant melon plants |
| CN107306788A (en) * | 2017-08-16 | 2017-11-03 | 镇江瑞繁农艺有限公司 | It is a kind of to obtain Cucumis wild species and the method for cultivation thick-skinned melon cenospecies |
-
2019
- 2019-06-20 CN CN201910535876.9A patent/CN110199869A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2274974A1 (en) * | 2005-12-21 | 2011-01-19 | Monsanto Invest N.V. | Closterovirus-resistant melon plants |
| CN107306788A (en) * | 2017-08-16 | 2017-11-03 | 镇江瑞繁农艺有限公司 | It is a kind of to obtain Cucumis wild species and the method for cultivation thick-skinned melon cenospecies |
Non-Patent Citations (4)
| Title |
|---|
| B KUBICK等: "Inheritance of some characters in muscmelons(Cucumis Melo L.)", 《GENETICA POLONICA》 * |
| 孙廷等: "《设施园艺生产技术》", 30 September 2015 * |
| 戴照义等: "《优质西瓜高效栽培技术》", 30 September 2010 * |
| 湖北省烟草科研所编: "《湖北省烟草生产技术培训教程》", 31 October 2013 * |
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Application publication date: 20190906 |