CN110179734A - A kind of reparation moisture retention liquid and facial mask liquid and preparation method thereof - Google Patents
A kind of reparation moisture retention liquid and facial mask liquid and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/0212—Face masks
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
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Abstract
The invention discloses a kind of reparation moisture retention liquids and facial mask liquid and preparation method thereof, it is intended in providing a kind of facial mask liquid containing Chinese herbal medicine extract for having both reparation and moisture-keeping efficacy, belong to cosmetic field.The facial mask liquid, is made of following components in parts by weight: moisture retention liquid 20-30 parts of reparation, 2-6 parts of compound amino acid, 0.1-0.5 parts of carboxymethyl chitosan, 0.2-0.6 parts of hydroxyethyl cellulose, 0.1-0.5 parts of allantoin, 1-5 parts of propylene glycol, 1-3 parts of pentanediol, 3-8 parts of glycerol, 0.1-0.5 parts of preservative and 70-90 parts of water.Facial mask liquid provided by the invention has the function of soothing the skin fatigue, inoxidizability while also having moisturizing, moisturizing；The facial mask liquid containing the Chinese herbal medicine extract is to no skin irritation, the phenomenon that long-time service is not in dermal toxicity, is a kind of health, multi-functional facial mask liquid；The cooperation of gynostemma pentaphylla, dendrobium candidum, extract of: pholidota chinesis lindl active constituent three's unique effects in facial mask liquid can play the good effects of nourishes rough skin.
Description
Technical field
The invention belongs to cosmetic technical fields, and in particular to a kind of reparation moisture retention liquid and facial mask liquid and preparation method thereof.
Background technique
In recent years, as the improvement of people's living standards, people increasingly pay attention to the nursing of skin, modern is to anti-ageing
Old beauty product is more paid attention to, so that demand of the market to anti-aging beauty product is more and more vigorous, every year all to compare faster
Example increases.
For facial mask as one of beauty product, having improves that skin effects are significant, advantage easy to use and deep consumed
Person's likes.Facial mask using when be to be covered on the non-woven fabrics mask for being soaked with facial mask liquid face 15-20 minutes, in this way can be temporary
When face be isolated with extraneous air and pollution, improve skin temperature, expand skin pore, promote sweat gland secretion and newly old generation
It thanks, increase the oxygen content of skin, be conducive to skin and exclude the product of epidermal cell metabolism and the oil substances of accumulation,
Facial mask liquid in pad pasting penetrates into the cuticula of skin epidermis, and skin is made to become soft, naturally bright and flexible.
However, the effect of facial mask on the market mainly pays attention to moisturizing, whitening, nti-freckle at present, but these facial masks often can
Add a large amount of thickener, such as card wave, triethanolamine substance and EDTA chelating agent and the anti-corrosion containing Form aldehyde release body
Agent, to skin, there are certain irritations.
Plant extracts is to extract to be made from pure natural plant, polysaccharide, amino acid, flavonoids and the soap contained
Glycosides isoreactivity substance has the effect of anti-oxidant, moisturizing, and is free from side effects.Therefore, containing the facial mask liquid of plant extracts
Increasingly by the favor of people seeking beauty.
Chinese patent CN 103385848B discloses a kind of toner containing sweet orange flower extract, can replenishing water and preserving moisture,
Nutrient is supplemented to skin, enhancing skin elasticity reduces wrinkle, and dry skin problem is prevented to occur repeatedly.Chinese patent application CN
104887609 A disclose a kind of moisture saver mask liquid, and the facial mask liquid is by water, glycerol, propylene glycol, Natrulon H-10, ethoxy
Urea, snail liquid, deep-sea salmon collagen, Herb Gynostemmae Pentaphylli extract, grass green salicornia europaeal extract, natto fermentation essence, oat β-
Glucan, carbomer, triethanolamine, Sodium Hyaluronate, methyl hydroxybenzoate, Phenoxyethanol and essence composition, the facial mask being prepared
Liquid can effective replenishing water and preserving moisture, be suitable for various skin.But the facial mask liquid has a single function, complex manufacturing technology, does not meet existing
For the requirement of people.Through retrieving, it is not yet found that the composition of dendrobium candidum extract, Herb Gynostemmae Pentaphylli extract, extract of: pholidota chinesis lindl,
And application of the composition in facial mask liquid.
Currently, there are many anti-aging and the method for maintenance skin, it mainly include external cosmetics and drug for oral administration.It uses now
Facial mask is a kind of maintenance method to skin.However, presently commercially available most of facial mask is in use, consumer can feel
Feel not ideal enough.To find out its cause, related with the constituent of its facial mask liquid and manufacture craft.
Curcurbitaceae gynostemma pentaphyllum genus herbaceous perennial vine plant dries all herbal medicine, medicinal material name gynostemma pentaphylla also known as seven with it
Leaf gallbladder, Herba Gynostemmatis, Herba Gynostemmatis, Pentapanax leschenaultii, Herb Gynostemmae Pentaphylli etc..Gynostemma pentaphylla contains saponin(e, flavones, polysaccharide, amino acid, microelement
Deng；Also containing organic acid, alkaloid, vitamin, terpene, phosphatide, protein etc..
Flavones has various effects in Herb Gynostemmae Pentaphylli extract, it is a kind of very strong antioxidant, can effectively remove in vivo
Free radical, it is this prevent oxidation ability be ten times of vitamin E or more, this antioxidation can prevent moving back for cell
Change, aging, the generation of cancer can also be prevented.Blood circulation can also be improved, cholesterol can be reduced, these effects substantially reduce
The disease incidence of cardiovascular and cerebrovascular disease, can also improve the symptom of cardiovascular and cerebrovascular disease.
Saponin(e in Herb Gynostemmae Pentaphylli extract have treat and prevent the cardiovascular diseases such as hypertension, diabetes, constipation, hemorrhoid,
Asthma, migraine, acne, color spot and other effects.Why " mystery " is because its panaxoside constituents is more than to gypenoside
The several times of ginseng.
Pharmacology and clinical research show that gynostemma pentaphylla is almost non-toxic, without side-effects, and there is anticancer, reducing blood lipid, anti-aging to make
With, body immune function can be enhanced, prevent and treat the side effect etc. of glucocorticoid.So gynostemma pentaphylla for cosmetics have it is very big before
Scape.Therefore Herb Gynostemmae Pentaphylli extract is used generally as drug, health care product.Chinese patent application CN 103191172A discloses strand
Application of the blue extract of stock in the cosmetics for preparing skin anti-light aging.Chinese patent application CN 102871924A is disclosed
Application of the Herb Gynostemmae Pentaphylli extract as moisturizer.
Dendrobium candidum chemical component multiplicity mainly has alkaloid, polysaccharide, amino acid, luxuriant and rich with fragrance class compound etc..Possess enhancing machine
Body immunity function, antitumor, anti-aging, antifatigue, hypoglycemic and other effects are potent tight by bioactivation moisturizing compound prescription
Skin is caused, 104127341 A of Chinese patent CN just illustrates dendrobium candidum and adjusts skin collagen metabolism or makeup in preparation
Application in product maintains enough collagenous fibres and elastomer, the aging of delaying skin.
Summary of the invention
It is had a single function to solve facial mask liquid presence in the prior art, the lower defect of safety, the purpose of the present invention exists
In providing a kind of tool anti-oxidant, moisturizing, moisturizing effect, and the higher facial mask liquid and its preparation containing natural extract of safety
Method.
The purpose of the present invention is what is be achieved through the following technical solutions:
A kind of reparation moisture retention liquid, is made of following raw material: 8-10 parts of dendrobium candidum extract, Herb Gynostemmae Pentaphylli extract
8-10 parts, 8-10 parts of extract of: pholidota chinesis lindl.
Further, the Herb Gynostemmae Pentaphylli extract is made by following methods:
(1) gynostemma pentaphylla is dry, then the gynostemma pentaphylla after weighing the drying of 10 parts by weight is ground, and is crossed 60 mesh and is obtained 6 weights
Measure part gynostemma pentaphylla powder, be dispersed in 150mL volume fraction be 75% ethanol solution in gynostemma pentaphylla suspension, make gynostemma pentaphylla with
The mass ratio of 75% ethanol solution is 1:25；
(2) cold after filtrate decompression is filtered after impregnating gynostemma pentaphylla suspension 2 hours in the water-bath that temperature is 80 DEG C
Hiding, after filter residue carries out aforesaid operations again, filtrate will carry out mixing to obtain gynostemma pentaphylla filtrate twice；
(3) ethyl alcohol is recycled by gynostemma pentaphylla filtrate in Rotary Evaporators heating evaporation, obtains concentrate, then puts
Enter baking oven to be dried to get gynostemma pentaphylla crude extract；
(4) the gynostemma pentaphylla crude extract after drying is dissolved in the distilled water of 100ml, obtains gynostemma pentaphylla crude extract solution,
It is to extract 15min under 1:1 room temperature by gynostemma pentaphylla crude extract solution and petroleum ether volume ratio；
It (5) again by volume will be to extract under 1:1 room temperature with ethyl acetate with the aqueous solution after petroleum ether extraction
15min obtains the B extract solution for dissolving in ethyl acetate；
(6) it will be extracted under 1:1 room temperature by volume again with ethyl acetate aqueous solution extracted with n-butanol
15min obtains the C extract solution for dissolving in n-butanol；
(7) the C extract solution of B extract solution and step (6) preparation by step (5) preparation is revolved respectively
Turn be evaporated to it is thick, by the thick shape extracting solution of each group be placed in drying box dry to water content less than 7%, obtain B extract and C
Extract and D extract, by B extract and C extract, 1:0.25-1.5 mixes to obtain Herb Gynostemmae Pentaphylli extract by volume.A, in D
Extract does not have inoxidizability；B, C extract has oxidation resistance stronger, therefore B extract and C extract can be used for maintenance guarantor
In wet liquid.
Further, the dendrobium candidum extract is made by following methods:
S1,60-75 parts by weight dendrobium candidum cleaned, is crushed, be scattered in distilled water, stirring is sufficiently mixed to obtain iron sheet stone
Dry measure used in former times suspension；The mass ratio of the dendrobium candidum and distilled water is 1:5-15；
S2,1.0-1.2 part by weight of cellulose enzyme is added under the conditions of 40-55 DEG C in dendrobium candidum suspension, stirring is filled
Divide reaction 2-5h；
S3, the hemicellulase that 0.7-1.0 parts by weight are added in the suspension of step S2, stirring, holding temperature are 40-
2-3h is sufficiently reacted under the conditions of 55 DEG C；
S4,0.6-1.2 parts by weight pectase, stirring are added in the suspension of step S3, holding temperature is 40-55 DEG C of item
1-2h is sufficiently reacted under part；
S5, the suspension for obtaining step S4 reduce temperature to 35-50 DEG C, and pH adjusting agent is added and adjusts pH to 4-6, is added
It is stirred after 0.6-1.2 parts by weight mannase, keeps 35-50 DEG C of temperature sufficiently reaction 2-3h；
S6, the stirring of 1.0-1.5 parts by weight neutral proteinase is added in the suspension that step S5 is obtained, holding temperature is
After 35-50 DEG C of sufficiently reaction 1-3h, filtering removes filter residue；
S7,120-150 parts by weight are concentrated the filtrate to get dendrobium candidum extract.
Further, the pH adjusting agent of the step S5 is citric acid
Further, 60 DEG C of the thickening temperature of the step S7, time 30min.
Further, the dendrobium candidum is fresh dendrobium candidum stem and leaf.
Further, extract of: pholidota chinesis lindl following methods are made:
A1, by 8 parts by weight Chinese pholidota pseudobulb or herbs it is dry after, ground 60 mesh obtains Chinese pholidota pseudobulb or herb coarse powder；
A2, Chinese pholidota pseudobulb or herb coarse powder is weighed, is dispersed in the ethanol solution that volume fraction is 80% and obtains Chinese pholidota pseudobulb or herb suspended matter, wherein
Chinese pholidota pseudobulb or herb and 80% ethanol solution mass ratio be 1:20；
A3, Chinese pholidota pseudobulb or herb suspended matter is put into microwave extraction tank, microwave power 300W, temperature is kept for 60 DEG C, is extracted
30min obtains Chinese pholidota pseudobulb or herb filtrate and Chinese pholidota pseudobulb or herb filter residue, then Chinese pholidota pseudobulb or herb filter residue repeats the above steps 2-4 times, is mentioned after filtering
It takes；
A4, the Chinese pholidota pseudobulb or herb Filtrate solutions for merging said extracted, obtain Chinese pholidota pseudobulb or herb crude extract；
A5, extract of: pholidota chinesis lindl is obtained after Chinese pholidota pseudobulb or herb crude extract to be carried out to rotary evaporation 15min under the conditions of 60 DEG C.
The invention also includes a kind of reparation moisture saver mask liquid containing gynostemma pentaphylla, according to parts by weight, by following raw material system
It is standby to form: to repair moisture retention liquid 20-30 parts, 2-6 parts of compound amino acid, 0.1-0.5 parts of carboxymethyl chitosan, hydroxyethyl cellulose
0.2-0.6 parts, 0.1-0.5 parts of allantoin, 1-5 parts of propylene glycol, 1-3 parts of pentanediol, 3-8 parts of glycerol, 0.1-0.5 parts of horse bp of outstanding person
With 70-90 parts of distilled water.
The invention also includes a kind of preparation methods of facial mask liquid as claimed in claim 8, comprising the following steps:
B1, distilled water is heated to 50-80 DEG C, allantoin is added, stirred to being completely dissolved, obtain allantoin liquid；
B2, carboxymethyl chitosan and hydroxyethyl cellulose are mixed, and stirred evenly, obtain the first mixed liquor；
B3, glycerol, propylene glycol and pentanediol are sequentially added in the first mixed liquor obtained to B2, is stirred evenly, obtain the
Two mixed liquors；
B4, the allantoin liquid that step B1 is obtained is uniformly mixed with the second mixed liquor that step B3 is obtained, is subsequently added into and repairs
Multiple moisture retention liquid, compound amino acid and Jie Ma bp, stir evenly to get facial mask liquid.
Beneficial effects of the present invention:
(1) facial mask liquid provided by the invention, which contains, repairs the plant extraction ingredients abundant such as moisture retention liquid, can be quickly by skin
Overall absorption gos deep into skin bottom and keeps the skin wet while water lock, hence it is evident that improves dry coarse skin condition, it is thin effectively to calm down skin
Line promotes skin metabolism, and pore refining, the improvement colour of skin is obscure, bright color, and sensitive skin of releiving, and keeps skin soft
Sliding delicate, rich in elastic force, brilliance shines；Energy delay skin aging is used for a long time, color spot is inhibited to achieve the purpose that the tender skin of bright face.It is
A kind of facial mask liquid meeting modern's requirement；
(2) for reparation moisture saver mask liquid provided by the invention to no skin irritation, long-time service is not in dermal toxicity
The phenomenon that, it is a kind of healthy, multi-functional facial mask liquid, is conducive to the large-scale production and sale of the facial mask liquid.
(3) manufacture craft difficulty of the invention is small, and step is simple, and economic cost is few, can industrialized production, be conducive to promote
Using.
(4) mannase main function is: reducing viscosity.Hemicellulase main function is: hemicellulase can be with
Decomposing the polysaccharide in dendrobium candidum is oligosaccharide, reduces viscosity；Pectase main function is: the crushing juice rate of dendrobium candidum is improved,
Accelerate the filtering of dendrobium candidum suspension, promotes clarification.Neutral proteinase main function is: by the macro-molecular protein of dendrobium candidum
It is hydrolyzed into small-molecular peptides or amino acid.
Detailed description of the invention
Fig. 1 is gynostemma pentaphylla and the vitamin C inoxidizability curve of 1 method of embodiment preparation.
Specific embodiment
In order to better understand the present invention, below by embodiment to the present invention into further explanation, embodiment is served only for
It explains the present invention, any restriction can't be constituted to the present invention.
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..Unless otherwise specified, embodiment
Used in the conventional means that are well known to those skilled in the art of technological means, raw materials used is commercial goods.
Embodiment 1:
A kind of reparation moisture saver mask liquid is grouped as by the group of following weight percent:
10 parts by weight of dendrobium candidum extract, gynostemma pentaphylla take 10 parts by weight of object, 8 parts by weight of extract of: pholidota chinesis lindl, compound ammonia
5 parts by weight of base acid, 0.3 parts by weight of carboxymethyl chitosan, 0.5 parts by weight of hydroxyethyl cellulose, 0.3 parts by weight of allantoin, the third two
3 parts by weight of alcohol, 1 parts by weight of pentanediol, 6 parts by weight of glycerol, outstanding horse bp (0.1 parts by weight of Phenoxyethanol and Sensiva SC50 0.1
Parts by weight) 0.2 parts by weight and 70 parts by weight distilled water.
A kind of preparation method of facial mask liquid, comprising the following steps:
B1, distilled water is heated to 50 DEG C, allantoin is added, stirred to being completely dissolved, obtain allantoin liquid；
B2, carboxymethyl chitosan and hydroxyethyl cellulose are mixed, and stirred evenly, obtain the first mixed liquor；
B3, glycerol, propylene glycol and pentanediol are sequentially added in the first mixed liquor obtained to B2, is stirred evenly, obtain the
Two mixed liquors；
B4, the allantoin liquid that step B1 is obtained is uniformly mixed with the second mixed liquor that step B3 is obtained, is subsequently added into and repairs
Multiple moisture retention liquid, compound amino acid and Jie Ma bp, stir evenly to get facial mask liquid.
The extract of: pholidota chinesis lindl following methods are made:
A1, by Chinese pholidota pseudobulb or herb it is dry after, ground 60 mesh obtains Chinese pholidota pseudobulb or herb coarse powder；
A2, Chinese pholidota pseudobulb or herb coarse powder is weighed, is dispersed in the ethanol solution that volume fraction is 80% and obtains Chinese pholidota pseudobulb or herb suspended matter, it is described
Chinese pholidota pseudobulb or herb coarse powder and 80% ethanol solution mass ratio be 1:20；
A3, Chinese pholidota pseudobulb or herb suspended matter is put into microwave extraction tank, microwave power 300W, temperature is kept for 60 DEG C, is extracted
30min obtains Chinese pholidota pseudobulb or herb filtrate and Chinese pholidota pseudobulb or herb filter residue, then Chinese pholidota pseudobulb or herb filter residue repeats the above steps 2 times, is mentioned after filtering
It takes；
A4, the Chinese pholidota pseudobulb or herb Filtrate solutions for merging said extracted, obtain Chinese pholidota pseudobulb or herb crude extract；
A5, extract of: pholidota chinesis lindl is obtained after Chinese pholidota pseudobulb or herb crude extract to be carried out to rotary evaporation 15min at 60 DEG C.
The dendrobium candidum extract is made by following methods:
S1,60 parts by weight dendrobium candidum stems and leaf are cleaned, are crushed, be scattered in distilled water, stirring is sufficiently mixed to obtain iron
Skin dendrobium nobile suspension；The mass ratio of the dendrobium candidum and distilled water is 1:5；
S2,1.0 part by weight of cellulose enzymes are added under the conditions of 40 DEG C in dendrobium candidum suspension, stir, sufficiently react
2h；
S3, the hemicellulase that 0.7 parts by weight are added in the suspension of step S2, stirring, holding temperature are 40 DEG C of items
2h is sufficiently reacted under part；
S4,0.6 parts by weight pectase is added in the suspension of step S3, stirs, holding temperature is filled under the conditions of being 40 DEG C
Divide reaction 1h；
S5, the suspension for obtaining step S4 reduce temperature to 35 DEG C, and lemon acid for adjusting pH is added to 4,0.6 weight is added
It is stirred after part mannase, keeps 35 DEG C of temperature sufficiently reaction 2h；
S6, the stirring of 1.0 parts by weight neutral proteinases is added in the suspension that step S5 is obtained, keeps temperature to be 35 DEG C and fills
After dividing reaction 1h, filtering removes filter residue；
S7,120 parts by weight are concentrated the filtrate to get dendrobium candidum extract；60 DEG C of time 30min of thickening temperature.
The Herb Gynostemmae Pentaphylli extract is made by following methods:
(1) gynostemma pentaphylla is dry, it is then ground, crosses 60 mesh, be dispersed in the ethanol solution that volume fraction is 75%
Gynostemma pentaphylla suspension, make it is dry after gynostemma pentaphylla with 75% ethanol solution mass ratio 1:25；
(2) cold after filtrate decompression is filtered after impregnating gynostemma pentaphylla suspension 2 hours in the water-bath that temperature is 80 DEG C
Hiding, after filter residue carries out aforesaid operations again, filtrate will carry out mixing to obtain gynostemma pentaphylla filtrate twice；
(3) gynostemma pentaphylla filtrate is evaporated into 15min under the conditions of 68 DEG C in Rotary Evaporators, ethanol evaporation obtains dense
Contracting liquid；Baking oven is put to be dried to get gynostemma pentaphylla crude extract；
(4) the gynostemma pentaphylla crude extract after drying is dissolved in the distilled water of 100ml, obtains gynostemma pentaphylla crude extract solution,
It is to extract 15min under 1:1 room temperature by gynostemma pentaphylla crude extract solution and petroleum ether volume ratio, the A for obtaining dissolving in petroleum ether is mentioned
Take object solution；
It (5) again by volume will be to extract under 1:1 room temperature with ethyl acetate with the aqueous solution after petroleum ether extraction
15min obtains the B extract solution for dissolving in ethyl acetate；
(6) it will be extracted under 1:1 room temperature by volume again with ethyl acetate aqueous solution extracted with n-butanol
15min obtains the C extract solution for dissolving in n-butanol；
(7) by D extract, A extract solution, B extract solution after step (6) extract in remaining aqueous solution
After carrying out rotary evaporation 10min with C extract solution, last solution is placed in 80 DEG C of drying boxes after drying 12 hours, is obtained
To A extract, B extract, C extract and D extract, by B extract and C extract, 1:0.25 mixes to obtain strand by volume
Blue extract.
Embodiment 2:
A kind of reparation moisture saver mask liquid is grouped as by the group of following weight percent:
8 parts by weight of dendrobium candidum extract, gynostemma pentaphylla take 8 parts by weight of object, 8 parts by weight of extract of: pholidota chinesis lindl, compounded amino
Sour 5 parts by weight, 0.3 parts by weight of carboxymethyl chitosan, 0.5 parts by weight of hydroxyethyl cellulose, 0.3 parts by weight of allantoin, propylene glycol 3
Parts by weight, 1 parts by weight of pentanediol, 6 parts by weight of glycerol, outstanding horse bp (0.1 weight of 0.1 parts by weight of Phenoxyethanol and Sensiva SC50
Measure part) 0.2 parts by weight and 70 parts by weight distilled water.
A kind of preparation method of facial mask liquid as claimed in claim 8, comprising the following steps:
B1, distilled water is heated to 70 DEG C, allantoin is added, stirred to being completely dissolved, obtain allantoin liquid；
B2, carboxymethyl chitosan and hydroxyethyl cellulose are mixed, and stirred evenly, obtain the first mixed liquor；
B3, glycerol, propylene glycol and pentanediol are sequentially added in the first mixed liquor obtained to B2, is stirred evenly, obtain the
Two mixed liquors；
B4, the allantoin liquid that step B1 is obtained is uniformly mixed with the second mixed liquor that step B3 is obtained, is subsequently added into and repairs
Multiple moisture retention liquid, compound amino acid and Jie Ma bp, stir evenly to get facial mask liquid.
The extract of: pholidota chinesis lindl following methods are made:
A1, by Chinese pholidota pseudobulb or herb it is dry after, ground 60 mesh obtains Chinese pholidota pseudobulb or herb coarse powder；
A2, Chinese pholidota pseudobulb or herb coarse powder is weighed, is dispersed in the ethanol solution that volume fraction is 80% and obtains Chinese pholidota pseudobulb or herb suspended matter, it is described
Chinese pholidota pseudobulb or herb coarse powder and 80% ethanol solution mass ratio be 1:20；
A3, Chinese pholidota pseudobulb or herb suspended matter is put into microwave extraction tank, microwave power 300W, temperature is kept for 60 DEG C, is extracted
30min obtains Chinese pholidota pseudobulb or herb filtrate and Chinese pholidota pseudobulb or herb filter residue, then Chinese pholidota pseudobulb or herb filter residue repeats the above steps 2 times, is mentioned after filtering
It takes；
A4, the Chinese pholidota pseudobulb or herb Filtrate solutions for merging said extracted, obtain Chinese pholidota pseudobulb or herb crude extract；
A5, Chinese pholidota pseudobulb or herb crude extract is obtained into extract of: pholidota chinesis lindl after 60 DEG C of progress rotary evaporation 15min.
The dendrobium candidum extract is made by following methods:
S1,70 parts by weight dendrobium candidum stems and leaf are cleaned, are crushed, be scattered in distilled water, stirring is sufficiently mixed to obtain iron
Skin dendrobium nobile suspension；The mass ratio of the dendrobium candidum and distilled water is 1:10；
S2,1.1 part by weight of cellulose enzymes are added under the conditions of 50 DEG C in dendrobium candidum suspension, stir, sufficiently react
3h；
S3, the hemicellulase that 0.8 parts by weight are added in the suspension of step S2, stirring, keeping temperature is 40-55 DEG C
Under the conditions of sufficiently react 2h；
S4,0.8 parts by weight pectase is added in the suspension of step S3, stirs, holding temperature is filled under the conditions of being 50 DEG C
Divide reaction 1h；
S5, the suspension for obtaining step S4 reduce temperature to 40 DEG C, and lemon acid for adjusting pH is added to 5,0.8 weight is added
It is stirred after part mannase, keeps 40 DEG C of temperature sufficiently reaction 2h；
S6, the stirring of 1.2 parts by weight neutral proteinases is added in the suspension that step S5 is obtained, keeps temperature to be 40 DEG C and fills
After dividing reaction 2h, filtering removes filter residue；
S7,135 parts by weight are concentrated the filtrate to get dendrobium candidum extract；60 DEG C of thickening temperature, time 30min.
The Herb Gynostemmae Pentaphylli extract is made by following methods:
(1) gynostemma pentaphylla is dry, it is then ground, crosses 60 mesh, be dispersed in the ethanol solution that volume fraction is 75%
Gynostemma pentaphylla suspension, make it is dry after gynostemma pentaphylla with 75% ethanol solution mass ratio 1:25；
(2) cold after filtrate decompression is filtered after impregnating gynostemma pentaphylla suspension 2 hours in the water-bath that temperature is 80 DEG C
Hiding, after filter residue carries out aforesaid operations again, filtrate will carry out mixing to obtain gynostemma pentaphylla filtrate twice；
(3) gynostemma pentaphylla filtrate is evaporated into 15min under the conditions of 68 DEG C in Rotary Evaporators, ethanol evaporation obtains dense
Contracting liquid；Baking oven is put to be dried to get gynostemma pentaphylla crude extract；
(4) the gynostemma pentaphylla crude extract after drying is dissolved in the distilled water of 100ml, obtains gynostemma pentaphylla crude extract solution,
It is to extract 15min under 1:1 room temperature by gynostemma pentaphylla crude extract solution and petroleum ether volume ratio, the A for obtaining dissolving in petroleum ether is mentioned
Take object solution；
It (5) again by volume will be to extract under 1:1 room temperature with ethyl acetate with the aqueous solution after petroleum ether extraction
15min obtains the B extract solution for dissolving in ethyl acetate；
(6) it will be extracted under 1:1 room temperature by volume again with ethyl acetate aqueous solution extracted with n-butanol
15min obtains the C extract solution for dissolving in n-butanol；
(7) by D extract, A extract solution, B extract solution after step (6) extract in remaining aqueous solution
After carrying out rotary evaporation 10min with C extract solution, last solution is placed in 80 DEG C of drying boxes after drying 12 hours, is obtained
To A extract, B extract, C extract and D extract, by B extract and C extract, 1:1 mixes to obtain gynostemma pentaphylla by volume
Extract.
Embodiment 3
A kind of reparation moisture saver mask liquid is grouped as by the group of following weight percent:
9 parts by weight of dendrobium candidum extract, gynostemma pentaphylla take 8 parts by weight of object, 9 parts by weight of extract of: pholidota chinesis lindl, compounded amino
Sour 3 parts by weight, 0.1 parts by weight of carboxymethyl chitosan, 0.6 parts by weight of hydroxyethyl cellulose, 0.5 parts by weight of allantoin, propylene glycol 2
Parts by weight, 3 parts by weight of pentanediol, 8 parts by weight of glycerol, outstanding horse bp (0.1 weight of 0.1 parts by weight of Phenoxyethanol and Sensiva SC50
Measure part) 0.2 parts by weight and 80 parts by weight distilled water.
A kind of preparation method of facial mask liquid as claimed in claim 8, comprising the following steps:
B1, distilled water is heated to 80 DEG C, allantoin is added, stirred to being completely dissolved, obtain allantoin liquid；
B2, carboxymethyl chitosan and hydroxyethyl cellulose are mixed, and stirred evenly, obtain the first mixed liquor；
B3, glycerol, propylene glycol and pentanediol are sequentially added in the first mixed liquor obtained to B2, is stirred evenly, obtain the
Two mixed liquors；
B4, the allantoin liquid that step B1 is obtained is uniformly mixed with the second mixed liquor that step B3 is obtained, is subsequently added into and repairs
Multiple moisture retention liquid, compound amino acid and Jie Ma bp, stir evenly to get facial mask liquid.
The extract of: pholidota chinesis lindl following methods are made:
A1, by Chinese pholidota pseudobulb or herb it is dry after, ground 60 mesh obtains Chinese pholidota pseudobulb or herb coarse powder；
A2, Chinese pholidota pseudobulb or herb coarse powder is weighed, is dispersed in the ethanol solution that volume fraction is 80% and obtains Chinese pholidota pseudobulb or herb suspended matter, it is described
Chinese pholidota pseudobulb or herb coarse powder and 80% ethanol solution mass ratio be 1:20；
A3, Chinese pholidota pseudobulb or herb suspended matter is put into microwave extraction tank, microwave power 300W, temperature is kept for 60 DEG C, is extracted
30min obtains Chinese pholidota pseudobulb or herb filtrate and Chinese pholidota pseudobulb or herb filter residue, then Chinese pholidota pseudobulb or herb filter residue repeats the above steps 2-4 times, is mentioned after filtering
It takes；
A4, the Chinese pholidota pseudobulb or herb Filtrate solutions for merging said extracted, obtain Chinese pholidota pseudobulb or herb crude extract；
A5, extract of: pholidota chinesis lindl is obtained after Chinese pholidota pseudobulb or herb crude extract to be carried out to rotary evaporation 15min at 60 DEG C.
The dendrobium candidum extract is made by following methods:
S1,75 parts by weight dendrobium candidum stems and leaf are cleaned, are crushed, be scattered in distilled water, stirring is sufficiently mixed to obtain iron
Skin dendrobium nobile suspension；The mass ratio of the dendrobium candidum and distilled water is 1:15；
S2,1.2 part by weight of cellulose enzymes are added under the conditions of 55 DEG C in dendrobium candidum suspension, stir, sufficiently react
5h；
S3, the hemicellulase that 1.0 parts by weight are added in the suspension of step S2, stirring, holding temperature are 55 DEG C of items
3h is sufficiently reacted under part；
S4,1.2 parts by weight pectases are added in the suspension of step S3, stir, holding temperature is filled under the conditions of being 55 DEG C
Divide reaction 2h；
S5, the suspension for obtaining step S4 reduce temperature to 50 DEG C, and lemon acid for adjusting pH is added to 6,1.2 weight are added
It is stirred after part mannase, temperature 50 C is kept sufficiently to react 3h；
S6, the stirring of 1.5 parts by weight neutral proteinases is added in the suspension that step S5 is obtained, keeps temperature to be 50 DEG C and fills
After dividing reaction 3h, filtering removes filter residue；
S7,150 parts by weight are concentrated the filtrate to get dendrobium candidum extract；60 DEG C of thickening temperature, time 30min.
The Herb Gynostemmae Pentaphylli extract is made by following methods:
(1) gynostemma pentaphylla is dry, it is then ground, crosses 60 mesh, be dispersed in the ethanol solution that volume fraction is 75%
Gynostemma pentaphylla suspension, make it is dry after gynostemma pentaphylla with 75% ethanol solution mass ratio 1:25；
(2) cold after filtrate decompression is filtered after impregnating gynostemma pentaphylla suspension 2 hours in the water-bath that temperature is 80 DEG C
Hiding, after filter residue carries out aforesaid operations again, filtrate will carry out mixing to obtain gynostemma pentaphylla filtrate twice；
(3) by gynostemma pentaphylla filtrate in Rotary Evaporators, 15min is evaporated under the conditions of 68 DEG C, ethanol evaporation obtains dense
Contracting liquid；Baking oven is put to be dried to get gynostemma pentaphylla crude extract；
(4) the gynostemma pentaphylla crude extract after drying is dissolved in the distilled water of 100ml, obtains gynostemma pentaphylla crude extract solution,
It is to extract 15min under 1:1 room temperature by gynostemma pentaphylla crude extract solution and petroleum ether volume ratio, the A for obtaining dissolving in petroleum ether is mentioned
Take object solution；
It (5) again by volume will be to extract under 1:1 room temperature with ethyl acetate with the aqueous solution after petroleum ether extraction
15min obtains the B extract solution for dissolving in ethyl acetate；
(6) it will be extracted under 1:1 room temperature by volume again with ethyl acetate aqueous solution extracted with n-butanol
15min obtains the C extract solution for dissolving in n-butanol；
(7) by D extract, A extract solution, B extract solution after step (6) extract in remaining aqueous solution
After carrying out rotary evaporation 10min with C extract solution, last solution is placed in 80 DEG C of drying boxes after drying 12 hours, is obtained
To A extract, B extract, C extract and D extract, by B extract and C extract, 1:1.5 mixes to obtain strand by volume
Blue extract.
Efficacy assessments
1. estimation of stability
(1) heat resistant test: facial mask fluid samples made of embodiment 1 are respectively charged into the test tube of 2 20mm × 120mm,
Make liquid level about 80mm, beyond the Great Wall clean rubber plug.The constant incubator being pre-adjusted to predetermined temperature is put into wherein one
In, after 24 hours, ready visual contrast is carried out with the facial mask liquid of another test tube after recovery room temperature.
(2) low temperature resistant test: facial mask fluid samples made of embodiment 1 are respectively charged into the test tube of 2 20mm × 120mm,
Make liquid level about 80mm, beyond the Great Wall clean rubber plug.Wherein one is put into and is pre-adjusted into the refrigerator of predetermined temperature, 24 is small
Shi Hou restores to carry out ready visual contrast with the facial mask liquid of another test tube after room temperature.
(3) heat-resisting cold-resistant alternating test: after 1 facial mask liquid of embodiment is carried out heat resistant test, low temperature resistant test is then carried out, such as
This is repeated to facial mask liquid and is layered.Record cycle-index.
(4) test result: showing after cold-resistant and heat-resistant experiment by range estimation, and the facial mask fluid samples of embodiment 1 are outside it
Sight will not change；After milk heat resists cold alternating test 100 times, facial mask liquid layering.
2. moisturizing is evaluated
(1) detection method: in vivo method
60 volunteers are looked for, between 25~45 years old age, 30 people of female, male 30 people.Random point 2 groups, and in identical environment
Under tested, in which:
S1 uses facial mask liquid 1 month or more of embodiment 1, all measures the moisture content of skin to them weekly and through the severe edema due to hypofunction of the spleen
Disperse vector two indices.
S2 is used common hyaluronic acid face mask liquid 1 month or more, equally all measures the moisture content of skin to them weekly
With percutaneous water loss amount two indices.
(2) testing result
It can thus be concluded that replenishing water and preserving moisture effect of the moisturizing moisturizing effect of facial mask liquid of the invention than common hyaluronic acid face mask liquid
It is more significant.
3. repair ability is evaluated
(1) using the anti-oxidant test of gynostemma pentaphylla of 1 method of embodiment preparation (compared with VC)
A. taking by solid-liquid ratio is 1:25 (g/ml), is impregnated 2 hours, is extracted 2 times in the water-bath that temperature is 80 with 75% ethyl alcohol
Extract vacuum drying after solid 0.01g is with the dissolution of the phosphate buffer of PH=6.8 and constant volume is in 25ml volumetric flask；
B. take above-mentioned solution 0,0.5,1.0,1.5,5ml in each 50ml volumetric flask；
C. it is separately added into 5ml 9mmol/L FeSO4Solution, 5ml 9mmol/L salicylic acid-ethanol solution；
D. 0.3% H is being added2O2Solution starting reaction, and distilled water is added to be settled to graduation mark, stand 40 minutes；
E. the absorbance (measurement is three times) of each solution is measured with extinction photometer, and calculates gynostemma pentaphylla to hydroxyl with absorbance
The clearance rate (clearance rate: A0-AX/A0) of free radical；
F. curve with clearance rate done with concentration and compared with the inoxidizability curve of VC；
G. experimental result: as shown in Figure 1, inoxidizability basically reaches unanimously both when concentration reaches 0.04mg/ml, says
It is bright high greater than 0.04mg/ml inoxidizability ratio VC when additive amount of the Herb Gynostemmae Pentaphylli extract in facial mask.
(2) the repair ability evaluation (promoting the experiment of fibrocyte functional examination) of facial mask liquid prepared by embodiment 1
A. instrument and reagent:
A. instrument: 96 orifice plates, enzyme-linked immunosorbent assay instrument, cell incubator, inverted phase contrast microscope, centrifuge, 60mm training
Support ware
B. reagent: pancreatin, repairs moisture saver mask liquid, DMSO, PBS liquid, MTT solution at skin fibroblasts
B. solution allocation:
A.5mg/ml MTT solution: MTT0.5g is weighed, is dissolved in 100ml phosphate buffer (PBS) or without phenol red culture medium
(60 DEG C of water-bath hydrotropies) is placed on 4 DEG C and is kept in dark place with 0.22um membrane filtration to remove the bacterium in solution.It (is configuring
During preservation, container is encased with aluminium-foil paper；It is ready-to-use)
B.PBS formula: NaCl 8g, KCl 0.2g, Na2HPO4 1.44g、KH2PO40.24g, PH to 7.4 is adjusted, be settled to
1ml
C. cell culture and processing:
A. human dermal fibroblast cell is seeded in the culture bottle equipped with appropriate culture solution, is placed in 37 DEG C of 5% dioxy
Change culture in carbon incubator, is grown in monolayer adherence.Every 1-2 days changes the liquid once, and every 3-4 days cell is broken when being bonded to 85%-90%,
Original fluid is discarded, PBS is rinsed 2-3 times, discards PBS, after adding 0.25% trypsase of people moderately to digest, trypsase is discarded,
Add people culture solution 10ml, pipette featheriness is beaten, and is therefrom drawn 5ml cell suspension inoculation in new culture bottle, is put in people's incubator
Culture.
B. the fibroblast in logarithmic growth state is taken, adjustment cell density is 1X106/ ml is inoculated in 96 orifice plates,
For 24 hours, after 50%-60% fusion, hungry 12h has reached cell synchronization for culture.Simultaneously be arranged zeroing hole (culture medium, MTT,
DMSO), well (cell, facial mask liquid, culture solution, MTT, DMSO), (cell, the facial mask liquid of same concentrations are molten for blank control wells
Solve medium, culture solution, MTT, DMSO), Positive control wells (cell, the HSF growth factor of same concentrations, culture solution, MTT,
DMSO), 5 parallel holes are set for every group.
C. it is placed in 37 DEG C of 5% carbon dioxide incubator and is incubated for 16-48h, observed under inverted microscope.
D. every hole adds 20 μ l MTT solution (5mg/ml, i.e. 0.5%MTT) of people, continues to cultivate 4h.If sample and MTT can
Reaction, can first be centrifuged and discard culture solution afterwards, carefully rush 2-3 after with PBS, then plus culture solution of the people containing MTT.
E. culture is terminated, culture solution in hole is carefully sucked.
F. every hole adds 150 μ l DMSO of people, sets low-speed oscillation 10min on shaking table, dissolves crystal sufficiently.Exempt from enzyme-linked
The light absorption value in each hole is measured at epidemic disease detector OD 490nm.
D. result calculates:
Every group of 5 parallel hole light absorption values are averaged, compare light absorption value size between each group, to judge sample to be tested
Facilitate fibrocyte proliferation ability.Experimental result is listed in the table below.
| Group | 1 | 2 | 3 | 4 | 5 | Average value |
| Zeroing hole | 0.1052 | 0.1205 | 0.1325 | 0.1087 | 0.1167 | 0.1167 |
| Well | 0.1213 | 0.097 | 0.1175 | 0.1045 | 0.1101 | 0.1101 |
| Blank control wells | 0.1353 | 0.1155 | 0.1230 | 0.1157 | 0.1224 | 0.1224 |
| Positive control wells | 0.1235 | 0.1363 | 0.1511 | 0.1115 | 0.1306 | 0.1306 |
Conclusion: MTT [3- (4,5- dimethylthiazole -2,5- diphenyltetrazoliumbromide) bromine] is water-soluble yellow stain, meeting
Dark blue crystals not soluble in water (MTT-formazan) is reduced to by NADPH in cell, it is first molten with DMSO (dimethyl alum)
Solution, then light absorption value is read with ferment immunoassay plate reading, if cell activity is stronger, blue is deeper；If cell death is presented
Yellow.Therefore by chart it is found that facial mask liquid has certain repair ability to skin fibroblasts.
The above description is only an embodiment of the present invention, is not intended to limit the scope of the invention, all to utilize this hair
Equivalent structure or equivalent flow shift made by bright description is applied directly or indirectly in other relevant technology necks
Domain is included within the scope of the present invention.
Claims (9)
1. a kind of reparation moisture retention liquid, which is characterized in that be made of following raw material: 8-10 parts of dendrobium candidum extract, twisting
Blue 8-10 parts of the extract of stock, 8-10 parts of extract of: pholidota chinesis lindl.
2. a kind of reparation moisture retention liquid according to claim 1, which is characterized in that the Herb Gynostemmae Pentaphylli extract is by following methods
It is made:
(1) gynostemma pentaphylla is dry, then the gynostemma pentaphylla after weighing the drying of 10 parts by weight is ground, and is crossed 60 mesh and is obtained 6 parts by weight
Gynostemma pentaphylla powder is dispersed in the ethanol solution that 150mL volume fraction is 75% and obtains gynostemma pentaphylla suspension, makes gynostemma pentaphylla and 75%
The mass ratio of ethanol solution is 1:25；
(2) it after impregnating gynostemma pentaphylla suspension 2 hours in the water-bath that temperature is 80 DEG C, refrigerates, filters after filtrate decompression is filtered
After slag carries out aforesaid operations again, filtrate it will carry out mixing to obtain gynostemma pentaphylla filtrate twice；
(3) ethyl alcohol is recycled by gynostemma pentaphylla filtrate in Rotary Evaporators heating evaporation, obtains concentrate, be then placed in baking
Case is dried to get gynostemma pentaphylla crude extract；
(4) the gynostemma pentaphylla crude extract after drying is dissolved in the distilled water of 100ml, obtains gynostemma pentaphylla crude extract solution, by strand
The blue crude extract solution of stock and petroleum ether volume ratio are to extract 15min under 1:1 room temperature；
(5) it again by volume will be to extract 15min under 1:1 room temperature with ethyl acetate with the aqueous solution after petroleum ether extraction, obtain
To the B extract solution for dissolving in ethyl acetate；
(6) it will be obtained with ethyl acetate aqueous solution extracted and n-butanol again by volume to extract 15min under 1:1 room temperature
To the C extract solution for dissolving in n-butanol；
(7) the C extract solution of B extract solution and step (6) preparation by step (5) preparation is subjected to rotation steaming respectively
It is sent to thick, the thick shape extracting solution of each group is placed in drying box and is dried to water content less than 7%, B extract and C is obtained and extracts
Object and D extract, by B extract and C extract, 1:0.25-1.5 mixes to obtain Herb Gynostemmae Pentaphylli extract by volume.
3. a kind of reparation moisture retention liquid according to claim 1, which is characterized in that the dendrobium candidum extract is by with lower section
Method is made:
S1,60-75 parts by weight dendrobium candidum is cleaned, is crushed, be scattered in distilled water, stirring be sufficiently mixed dendrobium candidum is outstanding
Supernatant liquid；The mass ratio of the dendrobium candidum and distilled water is 1:5-15；
S2,1.0-1.2 part by weight of cellulose enzyme is added under the conditions of 40-55 DEG C in dendrobium candidum suspension, stirring is sufficiently anti-
Answer 2-5h；
S3, the hemicellulase that 0.7-1.0 parts by weight are added in the suspension of step S2, stirring, keeping temperature is 40-55 DEG C
Under the conditions of sufficiently react 2-3h；
S4,0.6-1.2 parts by weight pectase, stirring, under the conditions of holding temperature is 40-55 DEG C are added in the suspension of step S3
Sufficiently reaction 1-2h；
S5, the suspension for obtaining step S4 reduce temperature to 35-50 DEG C, and pH adjusting agent is added and adjusts pH to 4-6,0.6- is added
It is stirred after 1.2 parts by weight mannases, keeps 35-50 DEG C of temperature sufficiently reaction 2-3h；
S6, the stirring of 1.0-1.5 parts by weight neutral proteinase is added in the suspension that step S5 is obtained, holding temperature is 35-50
DEG C sufficiently reaction 1-3h after, filtering, remove filter residue；
S7,120-150 parts by weight are concentrated the filtrate to get dendrobium candidum extract.
4. a kind of reparation moisture retention liquid according to claim 3, it is characterised in that: the pH adjusting agent of the step S5 is lemon
Acid.
5. a kind of reparation moisture retention liquid according to claim 3, it is characterised in that: 60 DEG C of the thickening temperature of the step S7,
Time 30min.
6. a kind of reparation moisture retention liquid according to claim 3, it is characterised in that: the dendrobium candidum is fresh iron sheet stone
Dry measure used in former times stem and leaf.
7. a kind of reparation moisture retention liquid according to claim 1, which is characterized in that extract of: pholidota chinesis lindl following methods are made:
A1, by Chinese pholidota pseudobulb or herb it is dry after, ground 60 mesh obtains Chinese pholidota pseudobulb or herb coarse powder；
A2,8 parts by weight Chinese pholidota pseudobulb or herb coarse powder are weighed, are dispersed in the ethanol solution that volume fraction is 80% and obtain Chinese pholidota pseudobulb or herb suspended matter,
Middle Chinese pholidota pseudobulb or herb and 80% ethanol solution mass ratio be 1:20；
A3, Chinese pholidota pseudobulb or herb suspended matter being put into microwave extraction tank, microwave power 300W, temperature is kept for 60 DEG C, 30min is extracted,
Chinese pholidota pseudobulb or herb filtrate and Chinese pholidota pseudobulb or herb filter residue are obtained after filtering, and then Chinese pholidota pseudobulb or herb filter residue repeats the above steps 2-4 times, extracts；
A4, the Chinese pholidota pseudobulb or herb Filtrate solutions for merging said extracted, obtain Chinese pholidota pseudobulb or herb crude extract；
A5, extract of: pholidota chinesis lindl is obtained after Chinese pholidota pseudobulb or herb crude extract to be carried out to rotary evaporation 15min under the conditions of 60 DEG C.
8. a kind of reparation moisture saver mask liquid containing gynostemma pentaphylla, it is characterised in that: according to parts by weight, prepared by following raw material and
At: any 20-30 parts of the reparation moisture retention liquid of claim 1-8,2-6 parts of compound amino acid, carboxymethyl chitosan 0.1-0.5
Part, 0.2-0.6 parts of hydroxyethyl cellulose, 0.1-0.5 parts of allantoin, 1-5 parts of propylene glycol, 1-3 parts of pentanediol, 3-8 parts of glycerol, outstanding person
0.1-0.5 parts and distilled water 70-90 parts of horse bp.
9. a kind of preparation method of facial mask liquid as claimed in claim 8, which comprises the following steps:
B1, distilled water is heated to 50-80 DEG C, allantoin is added, stirred to being completely dissolved, obtain allantoin liquid；
B2, carboxymethyl chitosan and hydroxyethyl cellulose are mixed, and stirred evenly, obtain the first mixed liquor；
B3, glycerol, propylene glycol and pentanediol are sequentially added in the first mixed liquor obtained to B2, is stirred evenly, it is mixed to obtain second
Close liquid；
B4, the allantoin liquid that step B1 is obtained is uniformly mixed with the second mixed liquor that step B3 is obtained, is subsequently added into reparation and protects
Wet liquid, compound amino acid and Jie Ma bp, stir evenly to get facial mask liquid.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111494238A (en) * | 2020-05-19 | 2020-08-07 | 贵州扬生医用器材有限公司 | Repairing and moistening mask containing carboxylated chitosan and preparation method thereof |
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| CN107722087A (en) * | 2017-08-22 | 2018-02-23 | 华南理工大学 | A kind of gynostemma pentaphylla flavone compound and its preparation and the application in antineoplastic |
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Application publication date: 20190830 |