CN110178838A - A kind of production method of green plants plasticizing sample - Google Patents
A kind of production method of green plants plasticizing sample Download PDFInfo
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- CN110178838A CN110178838A CN201910532858.5A CN201910532858A CN110178838A CN 110178838 A CN110178838 A CN 110178838A CN 201910532858 A CN201910532858 A CN 201910532858A CN 110178838 A CN110178838 A CN 110178838A
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- plastifying liquid
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
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Abstract
The present invention relates to a kind of production methods of green plasticizing sample comprising following steps: (1) prepared by plastifying liquid: PEG being configured to certain density plastifying liquid, sorbierite is added, dissolution adjusts plastifying liquid pH=6-8, spare；(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, carry out pure water and bathe immersion treatment；(3) it is plasticized: treated plant is all soaked in the plastifying liquid got ready, place and be plasticized in dark place, wipe plasticiser and obtain plasticizing sample；Method provided by the present invention is easy to operate, and manufacturing process is quick, safe and non-stimulating.Fusion cycle is short, can protect color by fidelity steadily in the long term.
Description
Technical field
The present invention relates to a kind of production methods of green plasticizing sample, belong to plant Techniques of preserving field.
Background technique
Medicinal plant sample is the related disciplines such as " Chinese pharmacology ", " medicinal botany ", " science of identification of Chinese materia medica ", " pharmacognosy ",
It is indispensable important referring to archival objects, it is teaching, indispensable component part in scientific research.Currently, common sample point
Following three types: exsiccata, dipping specimen and plasticizing sample.Exsiccata waxed plant leaves sample is by suppressing and being dried
Plant specimen, defect is fugitive color, broken and be easy to be damaged by insects, go mouldy, unsuitable long-term preservation.Dipping specimen be pass through by
Plant is soaked in after fixation treatment and saves the obtained sample of liquid, and defect is to save that liquid is often toxic, volatile, smell
Irritation is unfavorable for ornamental study.Plasticizing sample is favored due to the features such as its is pollution-free, form is lively and by people, and
Polyethylene glycol due to its is mild, compatibility is good, low toxicity is non-stimulated and be applied to plasticizing preparation of specimen in, in the prior art for
The report for being plasticized sample is as follows:
Publication No. CN1826887A discloses the plasticizing preparation method of animal and plant sample, includes sterilizing anti-corrosion, cleaning and plasticizing
Process is plasticized in process, and sample to be made gradually is soaked in concentration and incrementally increases by 10% plasticiser polyethylene glycol for 100%
In, the moisture in sample is replaced with polyethylene glycol.Its fabrication cycle is long, and the plasticizing of plant is not furtherd investigate.
Publication No. CN103548817A is related to the production method of plant plasticizing sample, and this method includes following plasticizing step
It is rapid: the plant after fixation to be successively soaked in the solution that 5 polyethylene glycol mass fractions are sequentially increased, and each adjacent two is molten
The difference of the polyethylene glycol mass fraction of liquid is in 15%-30%, until the polyethylene glycol mass fraction of last time solution is
100%；The time impregnated every time controlled at 5-20 hours, and the rear time impregnated is not less than the time of previous immersion.
A kind of method of CN107047545A green plants plasticizing sample and its application of obtained sample include fixation and
Plasticizing process, plasticizing specifically: the good green plants of fixation is put into 10~15h of immersion in 20% polyglycol solution, is connect
Be sequentially placed into 40% polyglycol solution 8~10h of immersion, impregnate 8~10h in 60% polyglycol solution, 80%
Polyglycol solution in impregnate 8~10h.
Above method is directed to the plasticizing of plant, is generally all carried out, was solidified by two steps of fixation treatment and plasticizing
Cheng Xuanyong inorganic solvent handles plant, such as Cu2+Acetum (provide acidic environment and Cu2+), principle is leaf
Piece Determination of Chlorophyll molecular structure is the porphyrin ring that magnesium ion is complexed, in acidic environment, H+Mg can be replaced2+, formed and go magnesium leaf green
Element, blade is brown, and de-magnesium pheophytin is easy to Cu2+In conjunction with forming more stable chloroplll cuprum, blade reverts to green again.
Manufacturing process uses glacial acetic acid more, has biggish irritation to operator, and copper ion is excessive using not environmentally.Plasticizing process
It is plasticized in such a way that gradient polyethylene glycol impregnates step by step, there is experimental implementation complexity, the defect of processing time length.
Summary of the invention
Technical problem to be solved by the invention is to provide it is a kind of it is easy to operate, fusion cycle is short, can protect steadily in the long term
The production method of the green plasticizing sample of Zhen Baose.
To solve the above problems, the technical solution used in the present invention is:
A kind of production method of green plasticizing sample comprising following steps:
(1) prepared by plastifying liquid: PEG being configured to certain density plastifying liquid, sorbierite is added, dissolution adjusts plastifying liquid pH=6-
8, it is spare；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, carry out pure water and bathe immersion treatment；
(3) it is plasticized: treated plant is all soaked in the plastifying liquid got ready, place and be plasticized in dark place, wipe modeling
Agent obtains plasticizing sample.
In some embodiment of the present invention, PEG configuration concentration is 40wt%-60wt% in step (1).
In some embodiment of the present invention, PEG is PEG200-600 in step (1).
In some embodiment of the present invention, the solvent of plastifying liquid is the pure water after degassing in step (1).At some
In the embodiment of invention, the additional proportion of sorbierite is 0.1-0.3g/5ml in step (1).
In some embodiment of the present invention, in step (2) pure water bathe temperature be 50-60 DEG C, processing the time be
60s-120s。
In some embodiment of the present invention, pure water bath is degassing pure water bath in step (2).
In some embodiment of the present invention, steps are as follows:
(1) prepared by plastifying liquid: the distilled water after PEG200-600 and degassing is configured to the plastifying liquid of 40%-60%, additional proportion
For the sorbierite of 0.1-0.3g/5ml, dissolution adjusts plastifying liquid pH=6-8；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, in the pure water bath after being placed in 50-60 DEG C of degassing
Handle 60s-120s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place, wipe modeling
Agent obtains plasticizing sample.
The beneficial effects of adopting the technical scheme are that
Method provided by the present invention is easy to operate, and manufacturing process is quick, safe and non-stimulating.Fusion cycle is short, can be steady for a long time
Determine fidelity and protects color.
The present invention influences the factor that chlorophyll decomposes from control in the fixation stage, obtains the chlorophyll in green plants sample
To save.Before plasticizing, the pure water bath processing of degassing of certain temperature, effectively destruction chlorophyllase are first used, plant senescence leaf is delayed
The plant physiologies phenomenon such as Huang completes plant fixation.The method is economic and environment-friendly, is not related to the replacement process of metal ion.
The present invention during plasticizing using the degassing plastifying liquid simultaneously dissolved with sorbierite and polyethylene glycol at
Reason, the time is short, can fast implement plasticizing, and production method is simpler, quick.
Detailed description of the invention
In order to illustrate more clearly of the specific embodiment of the invention or the effect of technical solution in the prior art, to provide
Attached drawing reference.
Fig. 1 is the plasticizing sample obtained using the method for the embodiment of the present invention 6.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, invention is carried out combined with specific embodiments below
Clear, complete description.
In following embodiment, it is 5% KOH and 0.1M HCl that pH, which adjusts agents useful for same,.
Embodiment 1
(1) prepared by plastifying liquid: the distilled water after PEG200 and degassing being configured to the plastifying liquid of 40wt% or so, additional proportion is
The sorbierite of 0.1g/5ml, if sorbierite not readily dissolves, can heating water bath be allowed to dissolve, adjust plastifying liquid pH=7；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, in the pure water bath after being placed in 50 ± 1 DEG C of degassings
Manage 60s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place；
(4) it saves: sample after plasticizing is taken out, wipe plastifying liquid, loading specimen bottle, when sample non-use is stored in dark place.
Embodiment 2
(1) prepared by plastifying liquid: the distilled water after PEG200 and degassing being configured to the plastifying liquid of 60wt% or so, additional proportion is
The sorbierite of 0.1g/5ml, if sorbierite not readily dissolves, can heating water bath be allowed to dissolve, adjust plastifying liquid pH=7；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, in the pure water bath after being placed in 50 ± 1 DEG C of degassings
Manage 90s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place；
(4) it saves: sample after plasticizing is taken out, wipe plastifying liquid, loading specimen bottle, when sample non-use is stored in dark place.
Embodiment 3
(1) prepared by plastifying liquid: the distilled water after PEG200 and degassing being configured to the plastifying liquid of 60wt% or so, additional proportion is
The sorbierite of 0.2g/5ml, if sorbierite not readily dissolves, can heating water bath be allowed to dissolve, adjust plastifying liquid pH=7；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, in the pure water bath after being placed in 50 ± 1 DEG C of degassings
Manage 120s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place；
(4) it saves: sample after plasticizing is taken out, wipe plastifying liquid, loading specimen bottle, when sample non-use is stored in dark place.
Embodiment 4
(1) prepared by plastifying liquid: the distilled water after PEG400 and degassing being configured to the plastifying liquid of 40wt% or so, additional proportion is
The sorbierite of 0.1g/5ml, if sorbierite not readily dissolves, can heating water bath be allowed to dissolve, adjust plastifying liquid pH=7；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, in the pure water bath after being placed in 55 ± 1 DEG C of degassings
Manage 60s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place；
(4) it saves: sample after plasticizing is taken out, wipe plastifying liquid, loading specimen bottle, when sample non-use is stored in dark place.
Embodiment 5
(1) prepared by plastifying liquid: the distilled water after PEG400 and degassing being configured to the plastifying liquid of 60wt% or so, additional proportion is
The sorbierite of 0.1g/5ml, if sorbierite not readily dissolves, can heating water bath be allowed to dissolve, adjust plastifying liquid pH=7；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, in the pure water bath after being placed in 55 ± 1 DEG C of degassings
Manage 90s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place；
(4) it saves: sample after plasticizing is taken out, wipe plastifying liquid, loading specimen bottle, when sample non-use is stored in dark place.
Embodiment 6
(1) prepared by plastifying liquid: the distilled water after PEG400 and degassing being configured to the plastifying liquid of 60wt% or so, additional proportion is
The sorbierite of 0.2g/5ml, if sorbierite not readily dissolves, can heating water bath be allowed to dissolve, adjust plastifying liquid pH=7；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, in the pure water bath after being placed in 55 ± 1 DEG C of degassings
Manage 120s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place；
(4) it saves: sample after plasticizing is taken out, wipe plastifying liquid, loading specimen bottle, when sample non-use is stored in dark place.
Embodiment 7
(1) prepared by plastifying liquid: the distilled water after PEG600 and degassing being configured to the plastifying liquid of 60wt% or so, additional proportion is
The sorbierite of 0.1g/5ml, if sorbierite not readily dissolves, can heating water bath be allowed to dissolve, adjust plastifying liquid pH=7；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, in the pure water bath after being placed in 55 ± 1 DEG C of degassings
Manage 60s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place；
(4) it saves: sample after plasticizing is taken out, wipe plastifying liquid, loading specimen bottle, when sample non-use is stored in dark place.
Embodiment 8
(1) prepared by plastifying liquid: the distilled water after PEG600 and degassing being configured to the plastifying liquid of 60wt% or so, additional proportion is
The sorbierite of 0.2g/5ml, if sorbierite not readily dissolves, can heating water bath be allowed to dissolve, adjust plastifying liquid pH=7；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, in the pure water bath after being placed in 55 ± 1 DEG C of degassings
Manage 90s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place；
(4) it saves: sample after plasticizing is taken out, wipe plastifying liquid, loading specimen bottle, when sample non-use is stored in dark place.
Embodiment 9
(1) prepared by plastifying liquid: the distilled water after PEG600 and degassing being configured to the plastifying liquid of 60wt% or so, additional proportion is
The sorbierite of 0.2g/5ml, if sorbierite not readily dissolves, can heating water bath be allowed to dissolve, adjust plastifying liquid pH=7；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, in the pure water bath after being placed in 55 ± 1 DEG C of degassings
Manage 120s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place；
(4) it saves: sample after plasticizing is taken out, wipe plastifying liquid, loading specimen bottle, when sample non-use is stored in dark place.
Embodiment 10
(1) prepared by plastifying liquid: the distilled water after PEG200 and degassing being configured to the plastifying liquid of 40wt% or so, additional proportion is
The sorbierite of 0.3g/5ml, if sorbierite not readily dissolves, can heating water bath be allowed to dissolve, adjust plastifying liquid pH=7；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, in the pure water bath after being placed in 60 ± 1 DEG C of degassings
Manage 60s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place；
(4) it saves: sample after plasticizing is taken out, wipe plastifying liquid, loading specimen bottle, when sample non-use is stored in dark place.
Embodiment 11
(1) prepared by plastifying liquid: the distilled water after PEG200 and degassing being configured to the plastifying liquid of 60wt% or so, additional proportion is
The sorbierite of 0.3g/5ml, if sorbierite not readily dissolves, can heating water bath be allowed to dissolve, adjust plastifying liquid pH=7；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, in the pure water bath after being placed in 60 ± 1 DEG C of degassings
Manage 120s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place；
(4) it saves: sample after plasticizing is taken out, wipe plastifying liquid, loading specimen bottle, when sample non-use is stored in dark place.
Embodiment 12
(1) prepared by plastifying liquid: the distilled water after PEG400 and degassing being configured to the plastifying liquid of 60wt% or so, additional proportion is
The sorbierite of 0.3g/5ml, if sorbierite not readily dissolves, can heating water bath be allowed to dissolve, adjust plastifying liquid pH=7；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, in the pure water bath after being placed in 60 ± 1 DEG C of degassings
Manage 60s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place；
(4) it saves: sample after plasticizing is taken out, wipe plastifying liquid, loading specimen bottle, when sample non-use is stored in dark place.
Embodiment 13
(1) prepared by plastifying liquid: the distilled water after PEG400 and degassing being configured to the plastifying liquid of 40wt% or so, additional proportion is
The sorbierite of 0.2g/5ml, if sorbierite not readily dissolves, can heating water bath be allowed to dissolve, adjust plastifying liquid pH=7；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, in the pure water bath after being placed in 60 ± 1 DEG C of degassings
Manage 120s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place；
(4) it saves: sample after plasticizing is taken out, wipe plastifying liquid, loading specimen bottle, when sample non-use is stored in dark place.
Embodiment 14
(1) prepared by plastifying liquid: the distilled water after PEG600 and degassing being configured to the plastifying liquid of 60wt% or so, additional proportion is
The sorbierite of 0.3g/5ml, if sorbierite not readily dissolves, can heating water bath be allowed to dissolve, adjust plastifying liquid pH=7；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, in the pure water bath after being placed in 60 ± 1 DEG C of degassings
Manage 60s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place；
(4) it saves: sample after plasticizing is taken out, wipe plastifying liquid, loading specimen bottle, when sample non-use is stored in dark place.
Embodiment 15
(1) prepared by plastifying liquid: the distilled water after PEG600 and degassing being configured to the plastifying liquid of 40wt% or so, additional proportion is
The sorbierite of 0.2g/5ml, if sorbierite not readily dissolves, can heating water bath be allowed to dissolve, adjust plastifying liquid pH=7；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, in the pure water bath after being placed in 60 ± 1 DEG C of degassings
Manage 120s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place；
(4) it saves: sample after plasticizing is taken out, wipe plastifying liquid, loading specimen bottle, when sample non-use is stored in dark place.
Embodiment 16
(1) prepared by plastifying liquid: the distilled water after PEG400 and degassing being configured to the plastifying liquid of 40wt% or so, additional proportion is
The sorbierite of 0.2g/5ml, if sorbierite not readily dissolves, can heating water bath be allowed to dissolve, adjust plastifying liquid pH=6；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, in the pure water bath after being placed in 55 ± 1 DEG C of degassings
Manage 60s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place；
(4) it saves: sample after plasticizing is taken out, wipe plastifying liquid, loading specimen bottle, when sample non-use is stored in dark place.
Embodiment 17
(1) prepared by plastifying liquid: the distilled water after PEG400 and degassing being configured to the plastifying liquid of 60wt% or so, additional proportion is
The sorbierite of 0.2g/5ml, if sorbierite not readily dissolves, can heating water bath be allowed to dissolve, adjust plastifying liquid pH=8；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, in the pure water bath after being placed in 55 ± 1 DEG C of degassings
Manage 120s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place；
(4) it saves: sample after plasticizing is taken out, wipe plastifying liquid, loading specimen bottle, when sample non-use is stored in dark place.
Comparative example 1
(1) prepared by plastifying liquid: the distilled water after PEG400 and degassing being configured to the plastifying liquid of 50wt% or so, adjusts plastifying liquid
pH=7；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, in the pure water bath after being placed in 55 ± 1 DEG C of degassings
Manage 90s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place；
(4) it saves: sample after plasticizing is taken out, wipe plastifying liquid, loading specimen bottle, when sample non-use is stored in dark place.
Comparative example 2
(1) prepared by plastifying liquid: the distilled water after PEG400 and degassing being configured to the plastifying liquid of 50wt% or so, additional proportion is
The glycerol of 0.2g/5ml adjusts plastifying liquid pH=7；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, in the pure water bath after being placed in 55 ± 1 DEG C of degassings
Manage 90s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place；
(4) it saves: sample after plasticizing is taken out, wipe plastifying liquid, loading specimen bottle, when sample non-use is stored in dark place.
Comparative example 3
(1) prepared by plastifying liquid: the distilled water after PEG400 and degassing being configured to the plastifying liquid of 50wt% or so, additional proportion is
The mannitol of 0.2g/5ml adjusts plastifying liquid pH=7；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, in the pure water bath after being placed in 55 ± 1 DEG C of degassings
Manage 90s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place；
(4) it saves: sample after plasticizing is taken out, wipe plastifying liquid, loading specimen bottle, when sample non-use is stored in dark place.
Comparative example 4
(1) prepared by plastifying liquid: the distilled water after PEG400 and degassing being configured to the plastifying liquid of 50wt% or so, additional proportion is
The sorbierite of 0.2g/5ml, if sorbierite not readily dissolves, can heating water bath be allowed to dissolve, measurement pH be 4
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, in the pure water bath after being placed in 55 ± 1 DEG C of degassings
Manage 90s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place；
(4) it saves: sample after plasticizing is taken out, wipe plastifying liquid, loading specimen bottle, when sample non-use is stored in dark place.
Comparative example 5
(1) prepared by plastifying liquid: PEG400 and water being configured to the plastifying liquid of 50wt% or so, additional proportion is the mountain of 0.2g/5ml
Pears alcohol, if sorbierite not readily dissolves, can heating water bath be allowed to dissolve, measurement pH be 10
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, in the pure water bath after being placed in 55 ± 1 DEG C of degassings
Manage 90s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place；
(4) it saves: sample after plasticizing is taken out, wipe plastifying liquid, loading specimen bottle, when sample non-use is stored in dark place.
Comparative example 6
(1) prepared by plastifying liquid: the distilled water after PEG400 and degassing being configured to the plastifying liquid of 30wt% or so, additional proportion is
The sorbierite of 0.2g/5ml, if sorbierite not readily dissolves, can heating water bath be allowed to dissolve, adjust plastifying liquid pH=7；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, in the pure water bath after being placed in 55 ± 1 DEG C of degassings
Manage 90s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place；
(4) it saves: sample after plasticizing is taken out, wipe plastifying liquid, loading specimen bottle, when sample non-use is stored in dark place.
Comparative example 7
(1) prepared by plastifying liquid: the distilled water after PEG400 and degassing being configured to the plastifying liquid of 70wt% or so, additional proportion is
The sorbierite of 0.2g/5ml, if sorbierite not readily dissolves, can heating water bath be allowed to dissolve, adjust plastifying liquid pH=7；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, in the pure water bath after being placed in 55 ± 1 DEG C of degassings
Manage 90s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place；
(4) it saves: sample after plasticizing is taken out, wipe plastifying liquid, loading specimen bottle, when sample non-use is stored in dark place.
Comparative example 8
(1) prepared by plastifying liquid: the distilled water after PEG400 and degassing being configured to the plastifying liquid of 50wt% or so, additional proportion is
The sorbierite of 0.05g/5ml, if sorbierite not readily dissolves, can heating water bath be allowed to dissolve, adjust plastifying liquid pH=7；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, in the pure water bath after being placed in 55 ± 1 DEG C of degassings
Manage 90s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place；
(4) it saves: sample after plasticizing is taken out, wipe plastifying liquid, loading specimen bottle, when sample non-use is stored in dark place.
Comparative example 9
(1) prepared by plastifying liquid: the distilled water after PEG400 and degassing being configured to the plastifying liquid of 50wt% or so, additional proportion is
The sorbierite of 0.4g/5ml, if sorbierite not readily dissolves, can heating water bath be allowed to dissolve, adjust plastifying liquid pH=7；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, in the pure water bath after being placed in 55 ± 1 DEG C of degassings
Manage 90s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place；
(4) it saves: sample after plasticizing is taken out, wipe plastifying liquid, loading specimen bottle, when sample non-use is stored in dark place.
Comparative example 10
(1) prepared by plastifying liquid: the distilled water after PEG400 and degassing being configured to the plastifying liquid of 50wt% or so, additional proportion is
The sorbierite of 0.2g/5ml, if sorbierite not readily dissolves, can heating water bath be allowed to dissolve, adjust plastifying liquid pH=7；
(2) it is plasticized: plant is all soaked in novel plastifying liquid, placed 24 hours in dark place；
(3) it saves: sample after plasticizing is taken out, wipe plastifying liquid, loading specimen bottle, when sample non-use is stored in dark place.
Comparative example 11
(1) prepared by plastifying liquid: the distilled water after PEG400 and degassing being configured to the plastifying liquid of 50wt% or so, additional proportion is
The sorbierite of 0.2g/5ml, if sorbierite not readily dissolves, can heating water bath be allowed to dissolve, adjust plastifying liquid pH=7；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, handled in the pure water bath after being placed in 45 DEG C of degassings
90s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place；
(4) it saves: sample after plasticizing is taken out, wipe plastifying liquid, loading specimen bottle, when sample non-use is stored in dark place.
Comparative example 12
(1) prepared by plastifying liquid: PEG400 and water being configured to the plastifying liquid of 50wt% or so, additional proportion is the mountain of 0.2g/5ml
Pears alcohol, if sorbierite not readily dissolves, can heating water bath be allowed to dissolve, adjust plastifying liquid pH=7；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, handled in the pure water bath after being placed in 65 DEG C of degassings
90s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place；
(4) it saves: sample after plasticizing is taken out, wipe plastifying liquid, loading specimen bottle, when sample non-use is stored in dark place.
Comparative example 13
(1) prepared by plastifying liquid: PEG400 being configured to the plastifying liquid of 50wt% or so with the distilled water that do not deaerate, additional proportion is
The sorbierite of 0.2g/5ml, if sorbierite not readily dissolves, can heating water bath be allowed to dissolve, adjust plastifying liquid pH=7；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, be placed in the 55 DEG C of pure water not deaerated baths and handle
90s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place；
(4) it saves: sample after plasticizing is taken out, wipe plastifying liquid, loading specimen bottle, when sample non-use is stored in dark place.
1 hot test of effect example
The sample obtained for embodiment 1-17, comparative example 1-13 is placed 72 hours, under the conditions of 55 ± 2 DEG C of temperature to sample
Form investigated, the results are shown in Table 1.
1 high temperature test result of table
2 high humidity test result of effect example
Sample for obtaining for embodiment 1-17, comparative example 1-12 is put under 40 ± 2 DEG C of temperature, 93 ± 3% damp conditions
It sets 7 days, the form of each sample is investigated, the results are shown in Table 2
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although
Present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: it still may be used
To modify to technical solution documented by previous embodiment or equivalent replacement of some of the technical features；And
These are modified or replaceed, the spirit and model of technical solution of the embodiment of the present invention that it does not separate the essence of the corresponding technical solution
It encloses.
Claims (8)
1. a kind of production method of green plasticizing sample, which is characterized in that it includes the following steps:
(1) prepared by plastifying liquid: PEG being configured to certain density plastifying liquid, sorbierite is added, dissolution adjusts plastifying liquid pH=6-
8, it is spare；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, carry out pure water and bathe immersion treatment；
(3) it is plasticized: treated plant is all soaked in the plastifying liquid got ready, place and be plasticized in dark place, wipe modeling
Agent obtains plasticizing sample.
2. a kind of production method of green plasticizing sample according to claim 1, which is characterized in that PEG matches in step (1)
Setting concentration is 40wt%-60wt%.
3. a kind of production method of green plasticizing sample according to claim 1, which is characterized in that PEG is in step (1)
PEG200-600。
4. a kind of production method of green plasticizing sample according to claim 1, which is characterized in that plasticizing in step (1)
The solvent of liquid is the pure water after degassing.
5. a kind of production method of green plasticizing sample according to claim 1, which is characterized in that sorb in step (1)
The additional proportion of alcohol is 0.1-0.3g/5ml.
6. a kind of production method of green plasticizing sample according to claim 1, which is characterized in that pure water in step (2)
The temperature of bath is 50-60 DEG C, and the processing time is 60s-120s.
7. a kind of production method of green plasticizing sample according to claim 1, which is characterized in that pure water in step (2)
Bath is degassing pure water bath.
8. a kind of production method of green plasticizing sample according to claim 1, which is characterized in that steps are as follows:
(1) prepared by plastifying liquid: the distilled water after PEG200-600 and degassing is configured to the plastifying liquid of 40%-60%, additional proportion
For the sorbierite of 0.1-0.3g/5ml, dissolution adjusts plastifying liquid pH=6-8；
(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, in the pure water bath after being placed in 50-60 DEG C of degassing
Handle 60s-120s；
(3) it is plasticized: treated plant is all soaked in novel plastifying liquid, placed 24 hours in dark place, wipe modeling
Agent obtains plasticizing sample.
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| CN111337319A (en) * | 2020-02-13 | 2020-06-26 | 大连医科大学 | Water bath device for fault plasticized specimen and water bath hardening method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111337319A (en) * | 2020-02-13 | 2020-06-26 | 大连医科大学 | Water bath device for fault plasticized specimen and water bath hardening method thereof |
| WO2021159953A1 (en) * | 2020-02-13 | 2021-08-19 | 大连医科大学 | Water bath device for sectional plastinated specimen, and hardening method by water bathing |
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