CN110178484A - A method of promoting astragalus membranaceus seed fast-germination - Google Patents
A method of promoting astragalus membranaceus seed fast-germination Download PDFInfo
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- CN110178484A CN110178484A CN201910641357.0A CN201910641357A CN110178484A CN 110178484 A CN110178484 A CN 110178484A CN 201910641357 A CN201910641357 A CN 201910641357A CN 110178484 A CN110178484 A CN 110178484A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
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- Environmental Sciences (AREA)
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- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
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- Soil Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
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- Pretreatment Of Seeds And Plants (AREA)
Abstract
The present invention relates to seed processing technology field, in particular to a kind of method for promoting astragalus membranaceus seed fast-germination.This method comprises the following steps: astragalus membranaceus seed being soaked in seed treatment, 20~30h of immersion is protected from light；Seed treatment is the PEG6000 aqueous solution of quality concentration expressed in percentage by volume 22%~28%；It will be layered on the filter paper or gauze of wetting after astragalus membranaceus seed cleaning after immersion, be placed in seed germination box and sprout, keep wetting state in germination box.After the present invention is to astragalus membranaceus seed processing, the germination rate and speed of germinating of astragalus membranaceus seed are not only effectively raised, but also further promote the elongation of rhizome.
Description
Technical field
The present invention relates to seed processing technology field, in particular to a kind of method for promoting astragalus membranaceus seed fast-germination.
Background technique
Radix Astragali also known as astragalus have more than 2000 years history as medicinal so far, have enhancing body's immunity, protect
Liver, diuresis, anti-aging, resisting stress, decompression and wide antibacterial action.Due to a large amount of excavations in recent years, cause wild Radix Astragali
Quantity is sharply reduced, and has the danger for tending to extinction.For in response to the market demand, artificial growth means have to be taken.But Radix Astragali kind skin
Containing wax, grease and pectic substance, there is strong phenomenon, i.e. seed coat loses permeability, even if under suitable external environment
Can not imbibition sprout, seriously affect the yield and quality of Radix Astragali, become restrict Radix Astragali production principal element.
Seed sprouting is the critical stage that plant starts growth and development, and the key condition for promoting or seed being inhibited to sprout is
Extraneous ecological condition.Therefore, it needs to carry out seed before sowing strong to abolish processing.
105519280 A patent of application publication number CN discloses a kind of method that promotion astragalus membranaceus seed is sprouted, including will be yellow
Stilbene seed is pre-processed before sprouting, the pretreatment be by plant growth regulator 60-70%, fungicide 5-10%,
Insecticide 9-13%, Chinese medicine liquid 10-20% are formulated a kind of obtained pretreatment fluid and are sprayed at astragalus membranaceus seed surface.Chinese medicine liquid by
Infusion forms after following composition weight proportion: 15-20 parts of Radix Angelicae Sinensis, 10-15 parts of radix bupleuri, 10-12 parts of radix euphorbiae lantu, 8-10 parts of the tuber of stemona,
5-8 parts of Radix Glycyrrhizae, 3-5 parts of Chinese anise, 2-3 parts of leaves of Hawthorn, 1-2 parts of stir-bake to yellowish beans.Application publication number CN 107710956 A, CN
104798486 108719299 A patents of A, CN.Seed treatment complicated composition in these patents, it is cumbersome, and germinate
Rate needs to be further increased.
Summary of the invention
In view of this, the present invention provides a kind of methods for promoting astragalus membranaceus seed fast-germination.The processing method, Neng Gouyou
Sprouting and the root growth of astragalus membranaceus seed are imitated and rapidly promote, it is economical and practical, it is simple to be applicable in, it has a vast market space and answers
Use prospect.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of methods for promoting astragalus membranaceus seed fast-germination, and this method comprises the following steps:
Astragalus membranaceus seed is soaked in seed treatment, 20~30h of immersion is protected from light；Seed treatment is quality volume basis
The PEG6000 aqueous solution of concentration 22%~28%；
It will be layered on the filter paper or gauze of wetting after astragalus membranaceus seed cleaning after immersion, be placed in seed germination box and sprout,
The box that germinates is interior to keep wetting state.
Preferably, being protected from light immersion for 24 hours.
Preferably, seed treatment is the PEG6000 aqueous solution of quality concentration expressed in percentage by volume 25%.
Preferably, the number of plies of filter paper or gauze is 2~3 layers.
Preferably, sprouting being layered on the gauze of wetting after the astragalus membranaceus seed cleaning after immersion with being placed in seed germination box
Further comprised the steps of: between hair be rolled into again with the covering of 2~3 layers of gauze it is cylindric.
Preferably, the temperature sprouted is 24~28 DEG C.
Preferably, the temperature of sprouting is 25 DEG C.
Preferably, the temperature sprouted is alternating temperature, alternating temperature program are as follows: 22~24 DEG C/14~16 DEG C, every 12h rotation.
Preferably, alternating temperature program are as follows: 23 DEG C/15 DEG C.
Preferably, the time of sprouting is 5~8 days.
Preferably, cleaning, which is adopted, washes with water, and the number of cleaning is 3 times.
The present invention provides a kind of methods for promoting astragalus membranaceus seed fast-germination.This method comprises the following steps: by Radix Astragali
Seed is soaked in seed treatment, is protected from light 20~30h of immersion；Seed treatment is quality concentration expressed in percentage by volume 22%~28%
PEG6000 aqueous solution；It will be layered on the filter paper or gauze of wetting after astragalus membranaceus seed cleaning after immersion, be placed in seed germination box
Middle sprouting, germination box is interior to keep wetting state.The invention has the advantages that
After the present invention is to astragalus membranaceus seed processing, the germination rate and speed of germinating of astragalus membranaceus seed are not only effectively raised, and
And further promote the elongation of rhizome.
Seed treatment agent prescription of the present invention is simple, and securely and reliably, facility requirements are low, and operation is easy, suitable for mass production,
Astragalus membranaceus seed sprouting and production practices can be rapidly promoted, astragalus membranaceus seed germinating time can be shortened and promote root growth.
In component of the invention, seed germination rate is can be improved in the PEG6000 of suitable concentration, promotes plant establishment, is improved
The resistance of seed and seedling.The gas permeability of gauze ensure that the aerobic respiration of seed and the space of Radix Astragali root growth, gauze
Water imbibition advantage be sufficiently to ensure moisture needed for Radix Astragali germinates, and can be avoided rotten by bubble.Alternating temperature stratification processing
The seed dormancy of Partial Species can be broken, this is the experimental results showed that alternating temperature stratification processing is suitable for astragalus membranaceus seed.
Specific embodiment
The invention discloses a kind of method for promoting astragalus membranaceus seed fast-germination, those skilled in the art can be used for reference herein
Content is suitably modified realization of process parameters.In particular, it should be pointed out that all similar substitutions and modifications are to those skilled in the art
It is it will be apparent that they are considered as being included in the present invention for member.Method and application of the invention has passed through preferably real
Example is applied to be described, related personnel obviously can not depart from the content of present invention, in spirit and scope to method described herein
It is modified or appropriate changes and combinations with application, carrys out implementation and application the technology of the present invention.
The present invention provides a kind of methods for promoting astragalus membranaceus seed fast-germination, comprising the following steps:
(1) prepared by seed treatment: weighing PEG6000 25.00g in beaker, adds after distilled water stirs to clarify and shift
To 100ml volumetric flask and constant volume.
(2) seed treatment impregnates: by astragalus membranaceus seed with after distilled water repeated flushing 3 times, after being blotted with dry filter paper rapidly
Put 150ml triangular flask into.The seed treatment that step (1) is produced is added to be protected from light triangle bottle closure, impregnates 24 hours.
(3) alternating temperature stratification is handled: being cut 4 layers of the gauze (hospital gauze) of suitable size, is soaked with distilled water, by step
(2) treated, and astragalus membranaceus seed is placed on two layers of gauze with distilled water repeated flushing 3 times, then is rolled into two layers of gauze covering
It is cylindric, it is put into plastic casing after being fixed using rubber band, keeps suitable distilled water persistently to supply in box.Finally by plastic casing
It is put into growth cabinet, 23 DEG C/15 DEG C of temperature setting, every 12 hour rotations.
Preferably, PEG6000 concentration is 25%.
Preferably, inorganic agent soaking time is 24 hours.
Preferably, being handled using gauze device and 23 DEG C/15 DEG C alternating temperature stratifications.
Seed, reagent or material used can be by markets in the method provided by the invention for promoting astragalus membranaceus seed fast-germination
It buys.PEG6000 is purchased from Beijing DingGuo ChangSheng Biology Technology Co., Ltd.
Below with reference to embodiment, the present invention is further explained:
Embodiment 1
A method of promoting astragalus membranaceus seed fast-germination, comprising the following steps:
(1) prepared by seed treatment: weighing PEG6000 (Beijing DingGuo ChangSheng Biology Technology Co., Ltd) 25.00g
In beaker, adds after distilled water stirs to clarify and be transferred to 100ml volumetric flask and constant volume.
(2) seed treatment impregnates: by astragalus membranaceus seed with after distilled water repeated flushing 3 times, after being blotted with dry filter paper rapidly
Put 150ml triangular flask into.The seed treatment that step (1) is produced is added to be protected from light triangle bottle closure, impregnates 24 hours.
(3) filter paper, room temperature processing: 2 layers of filter paper of suitable size are cut, are soaked with distilled water, treated by step (2)
Astragalus membranaceus seed is placed on filter paper with distilled water repeated flushing 3 times, after be put into plastic casing, filter paper wetting state is kept in box.
Plastic casing is finally put into growth cabinet, 25 DEG C of temperature setting.
Embodiment 2
A method of promoting astragalus membranaceus seed fast-germination, comprising the following steps:
(1) prepared by seed treatment: weighing PEG6000 (Beijing DingGuo ChangSheng Biology Technology Co., Ltd) 25.00g
In beaker, adds after distilled water stirs to clarify and be transferred to 100ml volumetric flask and constant volume.
(2) seed treatment impregnates: by astragalus membranaceus seed with after distilled water repeated flushing 3 times, after being blotted with dry filter paper rapidly
Put 150ml triangular flask into.The seed treatment that step (1) is produced is added to be protected from light triangle bottle closure, impregnates 24 hours.
(3) gauze, room temperature processing: 4 layers of the gauze (hospital gauze) of suitable size is cut, is soaked with distilled water, by step
(2) treated, and astragalus membranaceus seed is placed on two layers of gauze with distilled water repeated flushing 3 times, then is rolled into two layers of gauze covering
It is cylindric, it is put into plastic casing after being fixed using rubber band, keeps suitable distilled water persistently to supply in box.Finally by plastic casing
It is put into growth cabinet, 25 DEG C of temperature setting.
Embodiment 3
(1) prepared by seed treatment: weighing PEG6000 (Beijing DingGuo ChangSheng Biology Technology Co., Ltd) 25.00g
In beaker, adds after distilled water stirs to clarify and be transferred to 100ml volumetric flask and constant volume.
(2) seed treatment impregnates: by astragalus membranaceus seed with after distilled water repeated flushing 3 times, after being blotted with dry filter paper rapidly
Put 150ml triangular flask into.The seed treatment that step (1) is produced is added to be protected from light triangle bottle closure, impregnates 24 hours.
(3) gauze, alternating temperature stratification processing: cutting 4 layers of the gauze (hospital gauze) of suitable size, soaked with distilled water, will
Step (2) treated astragalus membranaceus seed is placed on two layers of gauze with distilled water repeated flushing 3 times, then is covered with two layers of gauze
Be rolled into it is cylindric, using rubber band it is fixed after be put into plastic casing, keep suitable distilled water persistently to supply in box.It finally will modeling
Magazine is put into growth cabinet, and 23 DEG C/15 DEG C of temperature setting, every 12 hour rotations.
Test example 1
The astragalus membranaceus seed of full grains is selected, embodiment is impregnated 24 hours with seed treatment, control group distillation water logging
Bubble 24 hours.Three kinds of germination environment are respectively room temperature filter paper, room temperature gauze, 23 DEG C/15 DEG C alternating temperature gauzes.Embodiment and control group
Totally 6 processing, each processing seed amount is 50, is repeated three times, long every 24 hour record germination seed numbers and rhizome
It is long to calculate germination percentage, germinating energy and average rhizome for degree, continuous record 5 days.Test result is shown in Table 1:
1 germination of table statistics
| Group | Germination percentage (%) | Germinating energy (%) | Average root stem length (cm) |
| Embodiment 1 | 56.7 | 50.0 | 3.79 |
| Control group 1 | 40.0 | 38.3 | 3.75 |
| Embodiment 2 | 60.5 | 59.0 | 3.92 |
| Control group 2 | 51.6 | 50.0 | 3.84 |
| Embodiment 3 | 68.5 | 66.0 | 3.96 |
| Control group 3 | 58.5 | 58.5 | 3.81 |
In component of the invention, seed germination rate is can be improved in the PEG6000 of suitable concentration, promotes plant establishment, is improved
The resistance of seed and seedling.The gas permeability of gauze ensure that the aerobic respiration of seed and the space of Radix Astragali root growth, gauze
Water imbibition advantage be sufficiently to ensure moisture needed for Radix Astragali germinates, and can be avoided rotten by bubble.Alternating temperature stratification processing
The seed dormancy of Partial Species can be broken, this is the experimental results showed that alternating temperature stratification processing is suitable for astragalus membranaceus seed.
Test example 2
The astragalus membranaceus seed of full grains is selected, the present embodiment is impregnated 24 hours with various concentration seed treatment, control group
With distilled water immersion 24 hours.Germination environment is room temperature filter paper, embodiment and control totally 6 processing, each processing seed amount
It is 50, repeats three times, it is continuous to record 5 days every 24 hour record germination seed numbers and rhizome length, calculating germination percentage,
Germinating energy and average rhizome are long.Test result is shown in Table 2:
2 germination of table statistics
| Group | Germination percentage (%) | Germinating energy (%) | Average root stem length (cm) |
| 10%PEG6000 | 43.33 | 42.67 | 3.59 |
| 15%PEG6000 | 56.67 | 53.33 | 3.66 |
| 20%PEG6000 | 50.00 | 46.67 | 3.69 |
| 25%PEG6000 | 65.00 | 60.00 | 3.70 |
| 30%PEG6000 | 50.00 | 48.33 | 3.67 |
| H2O | 43.33 | 41.67 | 3.57 |
In the component of this test, the PEG6000 of 25% concentration is the optimization process of astragalus membranaceus seed germination, suitable concentration
Seed germination rate can be improved in PEG6000, promotes plant establishment, improves the resistance of seed and seedling.
Test example 3
The astragalus membranaceus seed of full grains is selected, embodiment was with distilled water immersion 24 hours.Three kinds of germination environment are respectively normal
Warm filter paper, room temperature gauze, room temperature sponge, temperature are 20 DEG C or so.Totally 3 processing, each processing seed amount are embodiment
It 50, repeats three times, every 24 hour record germination seed numbers and rhizome length, continuous record 5 days calculates germination percentage, germination
Gesture and average rhizome are long.Test result is shown in Table 3:
3 germination of table statistics
| Group | Germination percentage (%) | Germinating energy (%) | Average root stem length (cm) |
| Filter paper | 40 | 38.35 | 3.64 |
| Sponge | 36.67 | 36.65 | 3.52 |
| Gauze | 51.67 | 50.00 | 3.76 |
In the component of this test, Radix Astragali germinates optimization process for gauze device, and the gas permeability of gauze, ensure that seed has
The space of oxygen breathing and Radix Astragali root growth, the water imbibition advantage of gauze are moisture needed for capable of sufficiently ensureing Radix Astragali germination, and
And it can be avoided rotten by bubble.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of method for promoting astragalus membranaceus seed fast-germination, which comprises the steps of:
Astragalus membranaceus seed is soaked in seed treatment, 20~30h of immersion is protected from light；The seed treatment is quality volume basis
The PEG6000 aqueous solution of concentration 22%~28%；
It will be layered on the filter paper or gauze of wetting after astragalus membranaceus seed cleaning after immersion, be placed in seed germination box and sprout, germinate
Wetting state is kept in box.
2. the method according to claim 1, wherein described be protected from light immersion for 24 hours.
3. the method according to claim 1, wherein the seed treatment is quality concentration expressed in percentage by volume 25%
PEG6000 aqueous solution.
4. the method according to claim 1, wherein the number of plies of the filter paper or gauze is 2~3 layers.
5. the method according to claim 1, wherein the yarn that wetting will be layered on after the astragalus membranaceus seed cleaning after immersion
On cloth and be placed in seed germination box sprout between further comprise the steps of: covered again with 2~3 layers of gauze be rolled into it is cylindric.
6. the method according to claim 1, wherein the temperature of the sprouting is 24~28 DEG C.
7. the method according to claim 1, wherein the temperature of the sprouting is alternating temperature, alternating temperature program are as follows: 22~
24 DEG C/14~16 DEG C, every 12h rotation.
8. the method according to the description of claim 7 is characterized in that the alternating temperature program are as follows: 23 DEG C/15 DEG C.
9. the method according to claim 1, wherein the time of the sprouting is 5~8 days.
10. method according to any one of claim 1 to 9, which is characterized in that the cleaning, which is adopted, to be washed with water, and is cleaned
Number be 3 times.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111662143A (en) * | 2020-07-22 | 2020-09-15 | 中国农业科学院特产研究所 | Fertilizer special for astragalus membranaceus and fertilizing method for improving yield of astragalus membranaceus |
| CN113179704A (en) * | 2021-06-18 | 2021-07-30 | 东北农业大学 | Method for improving germination rate of Chinese medicinal material astragalus seeds |
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2019
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| JPS63268716A (en) * | 1987-04-11 | 1988-11-07 | ビーエーエスエフ・アクチエンゲゼルシヤフト | Water-soluble copolymer, manufacture and use |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111662143A (en) * | 2020-07-22 | 2020-09-15 | 中国农业科学院特产研究所 | Fertilizer special for astragalus membranaceus and fertilizing method for improving yield of astragalus membranaceus |
| CN111662143B (en) * | 2020-07-22 | 2022-05-31 | 中国农业科学院特产研究所 | Fertilizer special for astragalus membranaceus and fertilizing method for improving yield of astragalus membranaceus |
| CN113179704A (en) * | 2021-06-18 | 2021-07-30 | 东北农业大学 | Method for improving germination rate of Chinese medicinal material astragalus seeds |
| CN113179704B (en) * | 2021-06-18 | 2024-04-26 | 东北农业大学 | Method for improving germination rate of astragalus seeds of traditional Chinese medicinal materials |
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Application publication date: 20190830 |