CN110172169A - A kind of preparation method of food package film - Google Patents
A kind of preparation method of food package film Download PDFInfo
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- CN110172169A CN110172169A CN201910480053.0A CN201910480053A CN110172169A CN 110172169 A CN110172169 A CN 110172169A CN 201910480053 A CN201910480053 A CN 201910480053A CN 110172169 A CN110172169 A CN 110172169A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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Abstract
The present invention discloses a kind of preparation method of food package film, is related to Food Packaging technology field.After the preparation method of the food package film is the following steps are included: highland barley bran is mixed with water, wheat bran mixed liquor is made in sterilization treatment；The bacterium solution of aureobasidium pullulans is seeded in the wheat bran mixed liquor, fermentation liquid is cultivated to obtain；The fermentation liquid is centrifuged, collecting supernatant and ethyl alcohol is added into the supernatant makes to generate precipitating, collects precipitating；After deposition and purification processing, dry polyoses extract；After the polyoses extract is dissolved in water, glycerol is added and stirs evenly, casting solution is made, after the casting solution bed board, baking, obtains food package film.The present invention is directed to prepare a kind of food package film, have the advantages that mechanical performance and barrier characteristics are good.
Description
Technical field
The present invention relates to Food Packaging technology field, in particular to a kind of preparation method of food package film.
Background technique
Traditional food package film is mainly the synthetic plastics such as polyethylene, polypropylene, but these synthetic plastics are also increasingly
Non-degradable, the non-renewable and characteristics such as influence food safety are exposed, causes serious pollution to environment, and increase blowdown
The additional investment of processing.With the ideological raising of people, consumer more pursues environmental protection, safety, green, organic food
Packaging material, the food package film of edibility become the hot spot of current research.
A kind of food package film the most popular of research now is prepared by natural macromolecular, has edibility, biology
Degradability, good-looking appearance, certain barrier and barrier property, it is nontoxic and pollution-free, inexpensive the features such as, but due to its main structure
As the stronger polysaccharide molecule of hydrophily, this kind of food package films has the defect for hindering moist and bad mechanical property mostly.
In addition, highland barley have " three high two is low " nutritional ingredient form, i.e., high protein, high dietary-fiber, homovitamin,
The features such as low fat, low sugar, is widely used in the fields such as food, feed, brewing and medical treatment.With the rise of highland barley processing industry,
The by-product of highland barley products is also increase accordingly, and the highland barley bran after highland barley processing is equally rich in multiple nutritional components, but highland barley
Wheat bran is mostly used for the preparation of feed and fails to play its maximum value.
Summary of the invention
The main object of the present invention is to propose a kind of preparation method of food package film, it is intended to prepare a kind of food packaging
Film has the advantages that mechanical performance and barrier characteristics are good.
To achieve the above object, the present invention proposes a kind of preparation method of food package film, the system of the food package film
Preparation Method the following steps are included:
After highland barley bran is mixed with water, wheat bran mixed liquor is made in sterilization treatment；
The bacterium solution of aureobasidium pullulans is seeded in the wheat bran mixed liquor, fermentation liquid is cultivated to obtain；
The fermentation liquid is centrifuged, collecting supernatant and ethyl alcohol is added into the supernatant makes to generate precipitating, and it is heavy to collect
It forms sediment；
After deposition and purification processing, dry polyoses extract；
After the polyoses extract is dissolved in water, glycerol is added and stirs evenly, casting solution is made, the casting solution is spread
After plate, baking, food package film is obtained.
Optionally, described the bacterium solution of aureobasidium pullulans is seeded in the wheat bran mixed liquor, cultivate to obtain fermentation liquid
In step,
Inoculum concentration is 2~4 (v/v) %；And/or
The time of the culture is 2~5 days.
Optionally, described the bacterium solution of aureobasidium pullulans is seeded in the wheat bran mixed liquor, cultivate to obtain fermentation liquid
Before step further include:
Mutagenic and breeding is carried out to aureobasidium pullulans, obtains the mutagenic strain for not producing melanin；
The mutagenic strain is inoculated into fluid nutrient medium, after cultivating 24~36h, obtains the bacterium solution of aureobasidium pullulans.
Optionally, the step of mutagenic and breeding is carried out to aureobasidium pullulans, obtains the mutagenic strain for not producing melanin packet
It includes:
Bacteria suspension is made in aureobasidium pullulans；
Under gnotobasis, stirring condition, to 5~10s of the bacteria suspension ultraviolet irradiation；
It will be cultivated 3~5 days on the bacterial suspension inoculation to PDA culture medium after ultraviolet irradiation, obtain and do not produce melanin
Mutagenic strain.
Optionally, described that the mutagenic strain is inoculated into fluid nutrient medium, after cultivating 24~36h, must sprout short stalk
In the step of bacterium solution of mould, the fluid nutrient medium includes: 30~50 parts of sucrose, dipotassium hydrogen phosphate 3~6 according to parts by weight
Part, 0.5~2 part of sodium chloride, 0.1~0.5 part of bitter salt, 0.3~1 part of ammonium sulfate, 2~5 parts of yeast extract and distillation
800~1500 parts of water.
Optionally, described after mixing highland barley bran with water, the step of wheat bran mixed liquor is made in sterilization treatment, includes:
After highland barley bran is crushed, 100~200 meshes are crossed, highland barley bran powder is obtained；
2~5 parts of highland barley bran powder are taken, 40~100 parts of deionized waters are added, suspension is made；
After adjusting the suspension pH to 5~6.5, the sterilization treatment at 120~125 DEG C obtains wheat bran mixed liquor.
Optionally, described to be centrifuged the fermentation liquid, collecting supernatant and ethyl alcohol is added into the supernatant makes to generate
Precipitating, collecting the step of precipitating includes:
The fermentation liquid is centrifuged, supernatant is collected；
After ethyl alcohol is added into the supernatant, it is placed in 10~15h under 0~4 DEG C of environment, collects precipitating.
Optionally, after the processing by the deposition and purification, the step of dry polyoses extract in, the purification process
Comprise the following steps: removal starch removes removing protein and removal small molecule salt.
Optionally, it is described the polyoses extract is dissolved in water after, be added glycerol stir evenly, casting solution is made, by institute
After stating casting solution bed board, baking, in the step of obtaining food package film,
The mass ratio of the polyoses extract and glycerol is (0.1~0.4): (0.06~0.1)；And/or
The temperature of the baking is 30~50 DEG C.
In technical solution of the present invention, using aureobasidium pullulans fermentation highland barley bran, due to being rich in β-Portugal in highland barley bran
Glycan and the high polysaccharide of other nutritive values, beta glucan have swelling character, certain retention ability and gel characteristic, can be with
Improve the mechanical performance of film；It is extracellular linear more can to secrete a kind of water solubility when fermenting highland barley bran for aureobasidium pullulans simultaneously
Sugar-pulullan polysaccharide, pulullan polysaccharide can improve the film forming characteristics of film, and film is made to have good barrier characteristics.Utilize budding
The product of short stalk mold fermentation highland barley bran has both the nutrients that beta glucan, pulullan polysaccharide and other highland barley brans contain
Matter is suitable for market so that manufactured food package film not only has good mechanical performance and barrier characteristics, but also healthy and safe
It promotes.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
Other relevant attached drawings are obtained according to these attached drawings.
Fig. 1 is the flow diagram of an embodiment of the preparation method of food package film proposed by the present invention；
Fig. 2 is the flow diagram of another embodiment of the preparation method of food package film proposed by the present invention.
The embodiments will be further described with reference to the accompanying drawings for the realization, the function and the advantages of the object of the present invention.
Specific embodiment
It in order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below will be in the embodiment of the present invention
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, according to normal conditions or manufacturer builds
The condition of view carries out.Reagents or instruments used without specified manufacturer is the conventional production that can be obtained by commercially available purchase
Product.
It should be noted that the meaning of the "and/or" occurred in full text, including three schemes arranged side by side, with " A and/or B "
For, the scheme including A scheme or B scheme or A and B while satisfaction.In addition, technical solution between each embodiment can be with
It is combined with each other, but must be based on can be realized by those of ordinary skill in the art, when phase occurs in the combination of technical solution
Mutual contradiction or cannot achieve when, will be understood that the combination of this technical solution is not present, also not the present invention claims protection scope
Within.
A kind of food package film the most popular of research now is prepared by natural macromolecular, has edibility, biology
Degradability, good-looking appearance, certain barrier and barrier property, it is nontoxic and pollution-free, inexpensive the features such as, but due to its main structure
As the stronger polysaccharide molecule of hydrophily, this kind of food package films has the defect for hindering moist and bad mechanical property mostly.
In consideration of it, the present invention proposes a kind of preparation method of food package film, one can be prepared by the preparation method
Kind food package film may be used as food packaging, and the food package film has good mechanical performance and barrier characteristics, and
It is healthy and safe.The flow diagram of one embodiment of the preparation method of food package film as shown in connection with fig. 1, the food packaging
The preparation method of film the following steps are included:
Step S30, after mixing highland barley bran with water, wheat bran mixed liquor is made in sterilization treatment.
Wherein, the preferably white highland barley wheat bran of highland barley bran.
It is rich in beta glucan in highland barley, is the most crop of beta glucan content, average content in wheat crops in the world
It is 5%.But it has been investigated that beta glucan is mainly enriched in wheat bran in highland barley, and it is mainly distributed on gluten cell wall
In, in highland barley gluten cell wall containing about 26%.Beta glucan has swelling character, certain retention ability and gel characteristic, can
To improve the mechanical performance of film, good tensile strength, fracture elongation and sealing strength are made it have.In addition, highland barley bran
Also contain other polysaccharide and microelement in skin, using highland barley bran be substrate carry out fermentation can be made rich in beta glucan it is simultaneous its
The polyoses extract of his nutriment.
When it is implemented, step S30 may include:
Step S310, after crushing highland barley bran, 100~200 meshes is crossed, highland barley bran powder is obtained；
Step S320,2~5 parts of highland barley bran powder are taken, 40~100 parts of deionized waters are added, suspension is made；
Step S330, after adjusting the suspension pH to 5~6.5, the sterilization treatment at 120~125 DEG C obtains wheat bran mixing
Liquid.
Step S40, the bacterium solution of aureobasidium pullulans is seeded in the wheat bran mixed liquor, cultivates to obtain fermentation liquid.
Aureobasidium pullulans can secrete a kind of extracellular linear polysaccharide-pulullan polysaccharide of water solubility, Propiram in fermentation
Polysaccharide belongs to amorphous thick substances, and for structure using maltotriose as basic unit and by α -1,6 glycosidic bonds are this as connection
Unique linkage pattern imparts the film made of pulullan polysaccharide with good film forming characteristics: high water soluble, colourless nothing
Taste, transparent, flexibility, oil resistance, oxygen barrier.
In the present embodiment, the bacterium solution of aureobasidium pullulans is seeded in the wheat bran mixed liquor, fermentation liquid is cultivated to obtain.
Using highland barley bran as substrate, the same of pulullan polysaccharide can be made using aureobasidium pullulans fermentation highland barley bran
When extract beta glucan in highland barley bran, the advantages of when both food package film so obtained had not only had both each self film, but also
Can make up mutually to improve defect when each self film: the composite package film has colourless, tasteless, transparent, oil resistance, resistance oxygen
Property, heat-sealable, stronger tensile strength and flexibility, so that it, in fresh-keeping foodstuff, can extend the food fresh keeping phase；And
Its preferable dissolubility also makes it be more suitable for the packaging of the food such as instant noodles oil plant or powder；Meanwhile it also being opened for highland barley bran
New application approach has been warded off, highland barley availability is improved, has facilitated the further genralrlization of highland barley.
Wherein, when being inoculated with, the inoculum concentration of bacterium solution is 2~4 (v/v) %, that is, the bacterium solution volume being inoculated with is mixed for wheat bran
Close 2~4 (v/v) % of liquid product；Incubation time after inoculation is 2~5 days.Wherein one day is for 24 hours, and 2~5 days i.e. 48~
120h。
In addition, the bacterium solution of aureobasidium pullulans can be made using conventional method, i.e., it is the bacterial strain of aureobasidium pullulans is living
Change, cultivate so that bacterium solution is made, specific steps are not described further.In the present embodiment, the aureobasidium pullulans are purchased from Guangdong strain
Collection.
Step S50, the fermentation liquid is centrifuged, collects supernatant and ethyl alcohol is added into the supernatant keeps generation heavy
It forms sediment, collects precipitating.
When it is implemented, may include:
Step S510, the fermentation liquid is centrifuged, collects supernatant；
Step S520, after 0~4 DEG C of ethyl alcohol is added in Xiang Suoshu supernatant, it is placed in 10~15h under 0~4 DEG C of environment, is received
Collection precipitating.
Step S60, by after deposition and purification processing, dry polyoses extract.
Wherein, the purification process comprises the following steps: removal starch removes removing protein and removal small molecule salt.Wherein,
Removal starch goes removing protein and removal small molecule salt process there is no sequencing, also can be used and various conventional goes to clean
The method of matter can be using a variety of methods such as enzymatic hydrolysis, alcohol precipitation or dialysis by taking desizing as an example.
In the present embodiment, the removal starch be may comprise steps of:
Step S611, the calcium nitrate solution that mass fraction is 60~70% is added into the precipitating, obtains suspension；
Step S612, the suspension is placed in boiling water bath after 5~15min, under 3500~4500r/min revolving speed from
10~15min of the heart collects supernatant to get the concentrate of removal starch.
It is described that removing protein is gone to may comprise steps of:
Step S621, chloroform and n-butanol is added into the concentrate of the removal starch, is stood after vibrating 20~40min
10~15min, centrifuging and taking supernatant；
Step S622, the supernatant is taken to repeat step S621 3~5 times to get the concentrate for removing removing protein.
Wherein, the volume ratio of the concentrate of chloroform, n-butanol and removal starch is (4~5): 1:(25~30).
The removal small molecule salt the following steps are included:
Step S630, it after the precipitating being dissolved in water, is placed in the bag filter that molecular cut off is 12000~15000 thoroughly
Analysis 24~48h of processing, collects trapped fluid to get the concentrate of removal small molecule salt.
Further, step S630 can be carried out after removing starch, it may be assumed that the concentrate of the removal starch is placed in retention
24~48h of dialysis treatment in the bag filter that molecular weight is 12000~15000 collects trapped fluid to get the dense of removal small molecule salt
Contracting liquid.
Step S70, after the polyoses extract being dissolved in water, glycerol is added and stirs evenly, casting solution is made, by the casting
After film liquid bed board, baking, food package film is obtained.
In the present embodiment, the mass ratio of the polyoses extract and glycerol is (0.1~0.4): (0.06~0.1)；It is described
The temperature of baking is 30~50 DEG C.
Specifically, 0.1~0.4 part of polyoses extract is dissolved in 15~30 parts of water, 0.06~0.1 part of glycerol is added, stirs
After mixing uniformly, casting solution is made in deaeration processing；Casting solution is poured into mold, in 30~50 DEG C of baking ovens drying film forming to get
Food package film.
In addition, the bacterium solution of the aureobasidium pullulans in step S40 can also select aureobasidium pullulans mutagenic fungi
Bacterium solution, so as to which more preferable, the colourless food package film of transparency is made.Breeding first is carried out to aureobasidium pullulans, is obtained
Bacterium solution is made after more excellent bacterial strain, is used further to step S40.
In another embodiment of the invention, another embodiment process of preparation method of the present invention as shown in connection with fig. 2 is shown
It is intended to, further includes following steps S10 and step S20 before step S40:
Step S10, mutagenic and breeding is carried out to aureobasidium pullulans, obtains the mutagenic strain for not producing melanin.
By to strain mutagenesis breeding, can artificially obtain the strain excellent of needs, in the present embodiment, to short stalk of teething
Mould carries out mutagenic and breeding to select mutagenic strain excellent, without chromogenic element.In this way, it is colorless and transparent to orient acquisition
Food package film.In addition, there are many modes of mutagenic and breeding, can by irradiating various rays or microwave radiation breeding,
It can also promote to make a variation by adding mutagens, in the present embodiment, the mode of mutagenic and breeding is not construed as limiting, mutagenesis is only needed to go out
Bacterial strain is without chromogenic element.
For simplifying operating procedure, reducing the considerations of cost, in a wherein embodiment of the invention, step S10 passes through
As under type is realized:
Step S110, bacteria suspension is made in aureobasidium pullulans；
Step S120, under gnotobasis, stirring condition, to 5~10s of the bacteria suspension ultraviolet irradiation.
Wherein, agitating mode can be mechanical stirring, shaking table oscillation etc., in the present embodiment, the preferred mode of magnetic agitation.
Step S130, by the bacterial suspension inoculation after ultraviolet irradiation to PDA culture medium, (potato dextrose agar is trained
Support base) on cultivate 3~5 days, obtain the mutagenic strain for not producing melanin.
Step S20, it is inoculated with the mutagenic strain and cultivates, obtain the bacterium solution of aureobasidium pullulans.
When carrying out step S20, may include steps of:
The mutagenic strain is inoculated into fluid nutrient medium, after cultivating 24~36h, obtains the bacterium solution of aureobasidium pullulans；
Wherein, the fluid nutrient medium includes: 30~50 parts of sucrose, 3~6 parts of dipotassium hydrogen phosphate, chlorine according to parts by weight
Change 0.5~2 part of sodium, 0.1~0.5 part of bitter salt, 0.3~1 part of ammonium sulfate, 2~5 parts of yeast extract and distilled water 800
~1500 parts.
It should be noted that step S10, S20 and step S30 be without sequencing, it can be before step S30, Zhi Houhuo
Person carries out simultaneously, in Fig. 2, by taking step S10, S20, S30 sequence carries out as an example.
Technical solution of the present invention is described in further detail below in conjunction with specific embodiments and the drawings, it should be understood that
Following embodiment is only used to explain the present invention, is not intended to limit the present invention.
Embodiment 1
It after highland barley bran is crushed, sieves with 100 mesh sieve, obtains highland barley bran powder；2g highland barley bran powder is taken, 40g deionization is added
Suspension is made in water, after adjusting suspension pH to 5, in 120 DEG C of sterilization treatments, obtains wheat bran mixed liquor, spare.
By the 2% of the bacterium solution inoculation wheat bran mixeding liquid volume of aureobasidium pullulans into wheat bran mixed liquor, in 37 DEG C of constant temperature
2d is cultivated in incubator obtains fermentation liquid；Fermentation liquid is centrifuged, after collecting supernatant and 0~4 DEG C of ethyl alcohol being added into supernatant,
It is placed in 10h under 0~4 DEG C of environment, collects precipitating.
The calcium nitrate solution that mass fraction is 60~70% is added into the precipitating, is placed in boiling water bath after 10min, in
It is centrifuged 10min under 4000r/min revolving speed, collects supernatant to get the concentrate of removal starch；Into the concentrate of removal starch
Chloroform and n-butanol is added, stands 10min after vibrating 30min, centrifuging and taking supernatant is spare, and same method repeats precipitating 5 times, with
The concentrate of removing protein must be removed by removing protein；The concentrate for removing removing protein is placed in the bag filter that molecular cut off is 14000
Middle dialysis treatment 48h collects trapped fluid to get the concentrate of removal small molecule salt；The concentrate freezing of small molecule salt will be removed
It is dry, obtain polyoses extract.
After 0.1g polyoses extract is dissolved in water, 0.06g glycerol is added, after mixing evenly, casting solution is made in deaeration processing；
Casting solution is poured into mold, drying film forming is in 30 DEG C of baking ovens to get food package film.
Embodiment 2
After highland barley bran is crushed, 200 meshes are crossed, highland barley bran powder is obtained；3g highland barley bran powder is taken, 80g deionization is added
Suspension is made in water, after adjusting suspension pH to 5.5, in 121 DEG C of sterilization treatments, obtains wheat bran mixed liquor, spare.
By the 3% of the bacterium solution inoculation wheat bran mixeding liquid volume of aureobasidium pullulans into wheat bran mixed liquor, in 37 DEG C of constant temperature
3d is cultivated in incubator obtains fermentation liquid；Fermentation liquid is centrifuged, after collecting supernatant and 0~4 DEG C of ethyl alcohol being added into supernatant,
It is placed in 12h under 0~4 DEG C of environment, collects precipitating.
The calcium nitrate solution that mass fraction is 60~70% is added into the precipitating, is placed in boiling water bath after 15min, in
It is centrifuged 15min under 3500~4500r/min revolving speed, collects supernatant to get the concentrate of removal starch；To the dense of removal starch
Chloroform and n-butanol are added in contracting liquid, stands 12min after vibrating 20min, centrifuging and taking supernatant is spare, and same method repeats to precipitate
5 times, the concentrate of removing protein must be removed to remove protein；It is 14000 that the concentrate for removing removing protein, which is placed in molecular cut off,
Dialysis treatment 48h in bag filter collects trapped fluid to get the concentrate of removal small molecule salt；The concentration of small molecule salt will be removed
Liquid freeze-drying, obtains polyoses extract.
After 0.2g polyoses extract is dissolved in water, 0.07g glycerol is added, after mixing evenly, casting solution is made in deaeration processing；
Casting solution is poured into mold, drying film forming is in 35 DEG C of baking ovens to get food package film.
Embodiment 3
It after highland barley bran is crushed, sieves with 100 mesh sieve, obtains highland barley bran powder；5g highland barley bran powder is taken, 100g deionization is added
Suspension is made in water, after adjusting suspension pH to 6, in 121 DEG C of sterilization treatments, obtains wheat bran mixed liquor, spare.
By the 2.8% of the bacterium solution inoculation wheat bran mixeding liquid volume of aureobasidium pullulans into wheat bran mixed liquor, in 37 DEG C of perseverances
4d is cultivated in warm incubator obtains fermentation liquid；Fermentation liquid is centrifuged, supernatant is collected and 0~4 DEG C of ethyl alcohol is added into supernatant
Afterwards, it is placed in 14h under 0~4 DEG C of environment, collects precipitating.
The calcium nitrate solution that mass fraction is 60~70% is added into the precipitating, is placed in boiling water bath after 5min, in
It is centrifuged 10min under 3500r/min revolving speed, collects supernatant to get the concentrate of removal starch；Into the concentrate of removal starch
Chloroform and n-butanol is added, stands 15min after vibrating 40min, centrifuging and taking supernatant is spare, and same method repeats precipitating 5 times, with
The concentrate of removing protein must be removed by removing protein；The concentrate for removing removing protein is placed in the bag filter that molecular cut off is 14000
Middle dialysis treatment 48h collects trapped fluid to get the concentrate of removal small molecule salt；The concentrate freezing of small molecule salt will be removed
It is dry, obtain polyoses extract.
After 0.3g polyoses extract is dissolved in water, 0.08g glycerol is added, after mixing evenly, casting solution is made in deaeration processing；
Casting solution is poured into mold, drying film forming is in 40 DEG C of baking ovens to get food package film.
Embodiment 4
After highland barley bran is crushed, 120 meshes are crossed, highland barley bran powder is obtained；4g highland barley bran powder is taken, 60g deionization is added
Suspension is made in water, after adjusting suspension pH to 6.5, in 125 DEG C of sterilization treatments, obtains wheat bran mixed liquor, spare.
By the 4% of the bacterium solution inoculation wheat bran mixeding liquid volume of aureobasidium pullulans into wheat bran mixed liquor, in 37 DEG C of constant temperature
5d is cultivated in incubator obtains fermentation liquid；Fermentation liquid is centrifuged, after collecting supernatant and 0~4 DEG C of ethyl alcohol being added into supernatant,
It is placed in 15h under 0~4 DEG C of environment, collects precipitating.
The calcium nitrate solution that mass fraction is 60~70% is added into the precipitating, is placed in boiling water bath after 12min, in
It is centrifuged 10min under 3800r/min revolving speed, collects supernatant to get the concentrate of removal starch；Into the concentrate of removal starch
Chloroform and n-butanol is added, stands 13min after vibrating 30min, centrifuging and taking supernatant is spare, and same method repeats precipitating 5 times, with
The concentrate of removing protein must be removed by removing protein；The concentrate for removing removing protein is placed in the bag filter that molecular cut off is 14000
Middle dialysis treatment 48h collects trapped fluid to get the concentrate of removal small molecule salt；The concentrate freezing of small molecule salt will be removed
It is dry, obtain polyoses extract.
After 0.4g polyoses extract is dissolved in water, 0.1g glycerol is added, after mixing evenly, casting solution is made in deaeration processing；
Casting solution is poured into mold, drying film forming is in 50 DEG C of baking ovens to get food package film.
Embodiment 5
Bacteria suspension is made in aureobasidium pullulans, under gnotobasis, stirring condition, to the bacteria suspension ultraviolet irradiation
Then 5s will be cultivated 4 days on bacterial suspension inoculation to PDA culture medium, be obtained the mutagenic strain for not producing melanin, mutagenic strain is inoculated with
Into fluid nutrient medium, after culture for 24 hours, the bacterium solution of aureobasidium pullulans is obtained, it is spare.Wherein, the component of fluid nutrient medium includes:
Sucrose 30g, dipotassium hydrogen phosphate 6g, sodium chloride 0.5g, bitter salt 0.3g, ammonium sulfate 1g, yeast extract 2g and distilled water
800g。
It after highland barley bran is crushed, sieves with 100 mesh sieve, obtains highland barley bran powder；2g highland barley bran powder is taken, 40g deionization is added
Suspension is made in water, after adjusting suspension pH to 5, in 120 DEG C of sterilization treatments, obtains wheat bran mixed liquor, spare.
By the 2% of the bacterium solution inoculation wheat bran mixeding liquid volume of aureobasidium pullulans into wheat bran mixed liquor, in 37 DEG C of constant temperature
2d is cultivated in incubator obtains fermentation liquid；Fermentation liquid is centrifuged, after collecting supernatant and 0~4 DEG C of ethyl alcohol being added into supernatant,
It is placed in 10h under 0~4 DEG C of environment, collects precipitating.
The calcium nitrate solution that mass fraction is 60~70% is added into the precipitating, is placed in boiling water bath after 10min, in
It is centrifuged 10min under 4000r/min revolving speed, collects supernatant to get the concentrate of removal starch；Into the concentrate of removal starch
Chloroform and n-butanol is added, stands 10min after vibrating 30min, centrifuging and taking supernatant is spare, and same method repeats precipitating 5 times, with
The concentrate of removing protein must be removed by removing protein；The concentrate for removing removing protein is placed in the bag filter that molecular cut off is 14000
Middle dialysis treatment 48h collects trapped fluid to get the concentrate of removal small molecule salt；The concentrate freezing of small molecule salt will be removed
It is dry, obtain polyoses extract.
After 0.1g polyoses extract is dissolved in water, 0.06g glycerol is added, after mixing evenly, casting solution is made in deaeration processing；
Casting solution is poured into mold, drying film forming is in 30 DEG C of baking ovens to get food package film.
Embodiment 6
Bacteria suspension is made in aureobasidium pullulans, under gnotobasis, stirring condition, to the bacteria suspension ultraviolet irradiation
Then 8s will be cultivated 3 days on bacterial suspension inoculation to PDA culture medium, be obtained the mutagenic strain for not producing melanin, mutagenic strain is inoculated with
Into fluid nutrient medium, after cultivating 30h, the bacterium solution of aureobasidium pullulans is obtained, it is spare.Wherein, the component of fluid nutrient medium includes:
Sucrose 40g, dipotassium hydrogen phosphate 3g, sodium chloride 1.5g, bitter salt 0.1g, ammonium sulfate 0.8g, yeast extract 5g and distillation
Water 1500g.
After highland barley bran is crushed, 200 meshes are crossed, highland barley bran powder is obtained；3g highland barley bran powder is taken, 80g deionization is added
Suspension is made in water, after adjusting suspension pH to 5.5, in 121 DEG C of sterilization treatments, obtains wheat bran mixed liquor, spare.
By the 3.8% of the bacterium solution inoculation wheat bran mixeding liquid volume of aureobasidium pullulans into wheat bran mixed liquor, in 37 DEG C of perseverances
3.5d is cultivated in warm incubator obtains fermentation liquid；Fermentation liquid is centrifuged, supernatant is collected and 0~4 DEG C of second is added into supernatant
After alcohol, it is placed in 13h under 0~4 DEG C of environment, collects precipitating.
The calcium nitrate solution that mass fraction is 60~70% is added into the precipitating, is placed in boiling water bath after 15min, in
It is centrifuged 15min under 3500~4500r/min revolving speed, collects supernatant to get the concentrate of removal starch；To the dense of removal starch
Chloroform and n-butanol are added in contracting liquid, stands 12min after vibrating 20min, centrifuging and taking supernatant is spare, and same method repeats to precipitate
5 times, the concentrate of removing protein must be removed to remove protein；It is 14000 that the concentrate for removing removing protein, which is placed in molecular cut off,
Dialysis treatment 48h in bag filter collects trapped fluid to get the concentrate of removal small molecule salt；The concentration of small molecule salt will be removed
Liquid freeze-drying, obtains polyoses extract.
After 0.3g polyoses extract is dissolved in water, 0.07g glycerol is added, after mixing evenly, casting solution is made in deaeration processing；
Casting solution is poured into mold, drying film forming is in 32 DEG C of baking ovens to get food package film.
Embodiment 7
Bacteria suspension is made in aureobasidium pullulans, under gnotobasis, stirring condition, to the bacteria suspension ultraviolet irradiation
Then 7s will be cultivated 4.5 days on bacterial suspension inoculation to PDA culture medium, be obtained the mutagenic strain for not producing melanin, mutagenic strain be connect
Kind after cultivating 36h, obtains the bacterium solution of aureobasidium pullulans into fluid nutrient medium, spare.Wherein, the group subpackage of fluid nutrient medium
It includes: sucrose 35g, dipotassium hydrogen phosphate 4g, sodium chloride 2g, bitter salt 0.5g, ammonium sulfate 0.3g, yeast extract 4g and distillation
Water 1200g.
It after highland barley bran is crushed, sieves with 100 mesh sieve, obtains highland barley bran powder；5g highland barley bran powder is taken, 100g deionization is added
Suspension is made in water, after adjusting suspension pH to 6, in 121 DEG C of sterilization treatments, obtains wheat bran mixed liquor, spare.
By the 2.8% of the bacterium solution inoculation wheat bran mixeding liquid volume of aureobasidium pullulans into wheat bran mixed liquor, in 37 DEG C of perseverances
4d is cultivated in warm incubator obtains fermentation liquid；Fermentation liquid is centrifuged, supernatant is collected and 0~4 DEG C of ethyl alcohol is added into supernatant
Afterwards, it is placed in 14h under 0~4 DEG C of environment, collects precipitating.
The calcium nitrate solution that mass fraction is 60~70% is added into the precipitating, is placed in boiling water bath after 5min, in
It is centrifuged 10min under 3500r/min revolving speed, collects supernatant to get the concentrate of removal starch；Into the concentrate of removal starch
Chloroform and n-butanol is added, stands 15min after vibrating 40min, centrifuging and taking supernatant is spare, and same method repeats precipitating 5 times, with
The concentrate of removing protein must be removed by removing protein；The concentrate for removing removing protein is placed in the bag filter that molecular cut off is 14000
Middle dialysis treatment 48h collects trapped fluid to get the concentrate of removal small molecule salt；The concentrate freezing of small molecule salt will be removed
It is dry, obtain polyoses extract.
After 0.2g polyoses extract is dissolved in water, 0.08g glycerol is added, after mixing evenly, casting solution is made in deaeration processing；
Casting solution is poured into mold, drying film forming is in 40 DEG C of baking ovens to get food package film.
Embodiment 8
Bacteria suspension is made in aureobasidium pullulans, under gnotobasis, stirring condition, to the bacteria suspension ultraviolet irradiation
Then 10s will be cultivated 5 days on bacterial suspension inoculation to PDA culture medium, be obtained the mutagenic strain for not producing melanin, mutagenic strain be connect
Kind after cultivating 32h, obtains the bacterium solution of aureobasidium pullulans into fluid nutrient medium, spare.Wherein, the group subpackage of fluid nutrient medium
It includes: sucrose 50g, dipotassium hydrogen phosphate 5g, sodium chloride 1g, bitter salt 0.2g, ammonium sulfate 0.6g, yeast extract 3g and distillation
Water 1000g.
After highland barley bran is crushed, 160 meshes are crossed, highland barley bran powder is obtained；Take 4.5g highland barley bran powder, be added 70g go from
Suspension is made in sub- water, after adjusting suspension pH to 6.5, in 125 DEG C of sterilization treatments, obtains wheat bran mixed liquor, spare.
By the 4.2% of the bacterium solution inoculation wheat bran mixeding liquid volume of aureobasidium pullulans into wheat bran mixed liquor, in 37 DEG C of perseverances
5d is cultivated in warm incubator obtains fermentation liquid；Fermentation liquid is centrifuged, supernatant is collected and 0~4 DEG C of ethyl alcohol is added into supernatant
Afterwards, it is placed in 15h under 0~4 DEG C of environment, collects precipitating.
The calcium nitrate solution that mass fraction is 60~70% is added into the precipitating, is placed in boiling water bath after 12min, in
It is centrifuged 10min under 3800r/min revolving speed, collects supernatant to get the concentrate of removal starch；Into the concentrate of removal starch
Chloroform and n-butanol is added, stands 13min after vibrating 30min, centrifuging and taking supernatant is spare, and same method repeats precipitating 5 times, with
The concentrate of removing protein must be removed by removing protein；The concentrate for removing removing protein is placed in the bag filter that molecular cut off is 14000
Middle dialysis treatment 48h collects trapped fluid to get the concentrate of removal small molecule salt；The concentrate freezing of small molecule salt will be removed
It is dry, obtain polyoses extract.
After 0.4g polyoses extract is dissolved in water, 0.1g glycerol is added, after mixing evenly, casting solution is made in deaeration processing；
Casting solution is poured into mold, drying film forming is in 50 DEG C of baking ovens to get food package film.Comparative example 1
0.3g pulullan polysaccharide is weighed, 20mL water is added, the heating stirring 1.5h at 75 DEG C obtains pulullan polysaccharide solution.
0.06g glycerol is added into pulullan polysaccharide solution, after mixing evenly, casting solution is made in deaeration processing；By casting film
Liquid pours into mold, and drying film forming is in 30~50 DEG C of baking ovens to get food package film.
Content detection
The polyoses content in film that respectively prepared by detection Examples 1 to 8 as follows, and record as shown in table 1.
Beta glucan content: according to " the extracting and developing purifying of Jia Ying highland barley bran water solubility beta glucan and property are ground
Study carefully [D], East China University of Science, the method provided in 2013 " is detected；
Pulullan polysaccharide amount: it is detected according to QBT 4485-2013 " pulullan polysaccharide " method.
Polyoses content detects in 1 food package film of table
Performance test
The film prepared respectively to Examples 1 to 8 and comparative example carries out following performance test, and records result such as 2 institute of table
Show.
Detection project includes: that the moist, oxygen barrier of resistance, grease transit dose, molten water time, transparency, sealing strength, tension are strong
Degree.
Detection method:
Tensile strength: according to GB/T 1040.3-2006 " the measurement third portion of plastic tensile performance: film and thin slice
Experimental condition " method, is detected using TA physical property measurement instrument；
It hinders moist: according to GB1037-1988 " plastic film and sheet material water vapor permeability test method (cup type method) " method,
It is detected using moisture vapor transmission cup；
Oxygen barrier: according to ASTM D 3985-95 method, OX-TRAN2/21MD oxygen is applied under room temperature, condition of normal pressure
Transmission measurement instrument is detected.(constant incubator-chitosan/lentinan edible film performance study and characterization)
The molten water time: taking the membrane sample for being cut into 2cm × 2cm specification, is placed in the beaker for filling with 90 DEG C of water, starts to count
When, when observing in water under sunlight without particulate matter after a period of time, stop timing, film dissolution expends the time for the molten water time；
Grease transit dose: it is detected according to GB/T 16929-1997 " the saturating oiliness of packaging material test method " method；
Transparency: according to " Zavareze Eda, R, et al.Development of oxidised and heat-
Moisture treated potato starch film [J] .Food Chem, 2012.132 (1): provides in 344-50 "
Method is detected；
Sealing strength: according to QBT2358-1988 " plastic film packaging bag heat sealing strength test method " method, using TA
The detection of physical property measurement instrument.
2 performance test of table
As seen from the above table, food package film made from Examples 1 to 8 have the moist, oxygen barrier of good resistance, saturating oiliness,
Water solubility, transparency, sealing strength, tensile strength and fracture elongation, and hinder moist, tensile strength, transparency, fracture are prolonged
It stretches and is substantially better than comparative example on rate and sealing strength, it is better to illustrate that food package film prepared by preparation method of the present invention has
Mechanical performance and barrier characteristics.
The above is only a preferred embodiment of the present invention, is not intended to limit the scope of the invention, for this field
For technical staff, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any
Modification, equivalent replacement, improvement etc. should all be included within the scope of the present invention.
Claims (9)
1. a kind of preparation method of food package film, which comprises the following steps:
After highland barley bran is mixed with water, wheat bran mixed liquor is made in sterilization treatment；
The bacterium solution of aureobasidium pullulans is seeded in the wheat bran mixed liquor, fermentation liquid is cultivated to obtain；
The fermentation liquid is centrifuged, collecting supernatant and ethyl alcohol is added into the supernatant makes to generate precipitating, collects precipitating；
After deposition and purification processing, dry polyoses extract；
After the polyoses extract is dissolved in water, glycerol is added and stirs evenly, casting solution is made, by the casting solution bed board, dries
After system, food package film is obtained.
2. the preparation method of food package film as described in claim 1, which is characterized in that the bacterium by aureobasidium pullulans
Liquid is seeded in the wheat bran mixed liquor, cultivate fermentation liquid the step of in,
Inoculum concentration is 2~4 (v/v) %；And/or
The time of the culture is 2~5 days.
3. the preparation method of food package film as described in claim 1, which is characterized in that the bacterium by aureobasidium pullulans
Liquid is seeded in the wheat bran mixed liquor, cultivate fermentation liquid the step of before further include:
Mutagenic and breeding is carried out to aureobasidium pullulans, obtains the mutagenic strain for not producing melanin；
The mutagenic strain is inoculated into fluid nutrient medium, after cultivating 24~36h, obtains the bacterium solution of aureobasidium pullulans.
4. the preparation method of food package film as claimed in claim 3, which is characterized in that described to be carried out to aureobasidium pullulans
Mutagenic and breeding, the step of obtaining the mutagenic strain for not producing melanin include:
Bacteria suspension is made in aureobasidium pullulans；
Under gnotobasis, stirring condition, to 5~10s of the bacteria suspension ultraviolet irradiation；
It will be cultivated 3~5 days on the bacterial suspension inoculation to PDA culture medium after ultraviolet irradiation, obtain the mutagenesis for not producing melanin
Bacterial strain.
5. the preparation method of food package film as claimed in claim 3, which is characterized in that described to be inoculated with the mutagenic strain
Into fluid nutrient medium, after cultivating 24~36h, in the step of obtaining the bacterium solution of aureobasidium pullulans, the fluid nutrient medium is by weight
Amount number meter include: 30~50 parts of sucrose, 3~6 parts of dipotassium hydrogen phosphate, 0.5~2 part of sodium chloride, bitter salt 0.1~
0.5 part, 0.3~1 part of ammonium sulfate, 2~5 parts of yeast extract and 800~1500 parts of distilled water.
6. the preparation method of food package film as described in claim 1, which is characterized in that described to be mixed by highland barley bran and water
After conjunction, the step of wheat bran mixed liquor is made in sterilization treatment, includes:
After highland barley bran is crushed, 100~200 meshes are crossed, highland barley bran powder is obtained；
2~5 parts of highland barley bran powder are taken, 40~100 parts of deionized waters are added, suspension is made；
After adjusting the suspension pH to 5~6.5, the sterilization treatment at 120~125 DEG C obtains wheat bran mixed liquor.
7. the preparation method of food package film as described in claim 1, which is characterized in that it is described to be centrifuged the fermentation liquid,
Collect supernatant and into the supernatant be added ethyl alcohol make generate precipitating, collect precipitating the step of include:
The fermentation liquid is centrifuged, supernatant is collected；
After 0~4 DEG C of ethyl alcohol is added into the supernatant, it is placed in 10~15h under 0~4 DEG C of environment, collects precipitating.
8. the preparation method of food package film as described in claim 1, which is characterized in that described to handle the deposition and purification
Afterwards, in the step of dry polyoses extract, the purification process is comprised the following steps: removal starch removes removing protein and goes
Except small molecule salt.
9. the preparation method of food package film as described in claim 1, which is characterized in that described that the polyoses extract is molten
Yu Shuihou is added glycerol and stirs evenly, casting solution is made, after the casting solution bed board, baking, the step of obtaining food package film
In,
The mass ratio of the polyoses extract and glycerol is (0.1~0.4): (0.06~0.1)；And/or
The temperature of the baking is 30~50 DEG C.
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