CN110169290A - A kind of Matrix formulation procedure for preventing and treating orchid diseases - Google Patents
A kind of Matrix formulation procedure for preventing and treating orchid diseases Download PDFInfo
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- CN110169290A CN110169290A CN201910426013.8A CN201910426013A CN110169290A CN 110169290 A CN110169290 A CN 110169290A CN 201910426013 A CN201910426013 A CN 201910426013A CN 110169290 A CN110169290 A CN 110169290A
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- bacillus subtilis
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention discloses a kind of Matrix formulation procedures for preventing and treating orchid diseases, more particularly to field of agricultural production technologies, the matrix is mainly made using bacillus subtilis and pearl by main component, specific preparation process includes following operating procedure: step 1: select biologically active bacillus subtilis and perlite as raw material, it is spare；Step 2: it using bacillus subtilis biologically active in step 1 as bacterium germination strain, and is seeded in corresponding culture medium and is fermented using conventional method.The Matrix formulation procedure that the present invention prevents and treats orchid diseases is simple, easy to use, the bacillus subtilis that can be carried by perlite kills the various phytopathy originals, to achieve the effect that prevent and treat orchid diseases, active constituent bacillus subtilis used in the present invention belongs to probiotics simultaneously, the problems such as capable of avoiding environmental disruption, pathogen drug resistance caused by due to using chemical pesticide, while orchid plant strain growth can also be promoted.
Description
Technical field
The present invention relates to field of agricultural production technologies, it is more particularly related to a kind of base for preventing and treating orchid diseases
Matter preparation method.
Background technique
Orchid is one of the famous flower of China, and the fragrance of a flower of orchid is elegant noble and unsullied, and flower pattern small and exquisite exquisiteness is called and spends middle gentleman.
But after pest and disease damage occurs for orchid, influence very much colored ornamental value.The common disease of orchid has: southern blight, anthracnose, leaf
Blight and tiller maize ear rot.Wherein, orchid southern blight: morbidity is most heavy when watering overly moist or improper ventilation, has especially just planted children soon
Seedling is most vulnerable, and infection site can generate brown scab, can expand rapidly in the environment of high temperature and humidity, dark brown spot is finally presented；
Cymbidium anthracnose: too close, improper ventilation is planted, water stress or injured wound etc. are easily susceptible, and blue strain illness initial stage will appear bright
Aobvious brown recess dot, can then be gradually expanded to form circular irregular scab, scab center will appear bad when serious
Subcutaneous ulcer phenomenon；Causing The Leaf Blight of Orchid: blade tip generates black fleck, and irregular scab is gradually expanded into, and scab periphery forms dark brown,
Centre is in ficelle, and entire blade is spread when serious, fallen leaves of finally withering；The symptom of tiller maize ear rot: tiller bud and blade are betided
On, there is water soaking mode yellow fleck in bastem portion in initial stage, forms dirty-green boiled water after 2-3 days and scalds the big patch of shape, finally goes out
The existing rotten block of dark brown frowzy, serious to lead to the dead strain of rotten bud, for tiller maize ear rot in early summer overcast and rainy season, air humidity is higher,
Often occur, mainly by wound infection, such as touch folding, insect bite, this disease most easily occurs for Chinese cymbidium, sword-leaved cymbidium young shoot.
The patent of invention of 107094814 A of patent application publication CN discloses a kind of disease control medicament of orchid,
By weight, it is made of the following components: 10-15 parts of garlic, 15-20 parts of onion, 2-3 parts of ginger, 2-3 parts of sweet potato, niblet
23 parts, 1-2 parts of mushroom, 0.1-0.2 parts of Radix Codonopsis, 0.5-1 parts of fructus lycii, 1-2 parts of Radix zanthoxyli, 0.2-0.4 parts of radix bupleuri, jujube 0.5-1
Part, 810 parts of Dalmatian chrysanthemum, 1-2 parts of cordate houttuynia, 5-8 parts of wormwood, 3-5 parts of toad grass, 0.5-1 parts of tealeaves.One kind provided by the invention
The disease control medicament of orchid, to control efficiency and the common orchid diseases pesticide control effect phase on the market of orchid diseases
It is imitative, but the present invention uses pure plant preparation, and nonhazardous is safer, and initiative use paste medicament, easy to use, drug effect
Persistently, avoiding commercially available medicament needs timing to spray the inconvenience of wine, is worth of widely use.
But it is in actual use, still there is more disadvantage, such as used raw material type is more, and technique is cumbersome.
Summary of the invention
In order to overcome the drawbacks described above of the prior art, the embodiment of the present invention provides a kind of matrix system for preventing and treating orchid diseases
Preparation Method is sprayed on the perlite that code heap is placed in step 2 after being filtered by the bacillus subtilis bacterium solution that will be fermented,
It is placed in natural air drying in the ventilated environment without other miscellaneous bacterias, this preparation method is simple, easy to use, and can take by perlite
The bacillus subtilis of band kills the various phytopathy originals, to achieve the effect that prevent and treat orchid diseases, while used in the present invention
Active constituent bacillus subtilis belongs to probiotics, can avoid environmental disruption, pathogen caused by due to using chemical pesticide
The problems such as drug resistance, while orchid plant strain growth can also be promoted, to solve the problems mentioned in the above background technology.
To achieve the above object, the invention provides the following technical scheme: it is a kind of prevent and treat orchid diseases Matrix formulation procedure,
The matrix is mainly made using bacillus subtilis and pearl by main component, and specific preparation process includes following operating procedure:
Step 1: selecting biologically active bacillus subtilis and perlite as raw material, spare；
Step 2: using bacillus subtilis biologically active in step 1 as bacterium germination strain, and conventional method is used
It is seeded in corresponding culture medium and is fermented；
Step 3: carrying out the placement of code heap for the perlite in step 1, be placed in the environment of the ventilation without other miscellaneous bacterias,
Give over to inoculating strain use；
Step 4: it after the bacillus subtilis bacterium solution fermented in step 2 filtering, is sprayed on code in step 2 and stacks
On the perlite set, it is placed in natural air drying in the ventilated environment without other miscellaneous bacterias, can be prepared by the matrix of prevention and treatment orchid diseases.
In a preferred embodiment, the bacillus subtilis in the step 1 be deposit number is ACCC-
10619 bacillus subtilis.
In a preferred embodiment, raw material percentage in the step 1, each component are as follows: have
The bacillus subtilis 1.00-20.00% of bioactivity, perlite 99-80%.
In a preferred embodiment, culture medium raw material used in the fermentation of bacillus subtilis be beancake powder,
Corn flour, potassium dihydrogen phosphate and water.
In a preferred embodiment, each component is by weight in culture medium used in the fermentation of bacillus subtilis
Percentage meter: beancake powder 10%, corn flour 10%, potassium dihydrogen phosphate 0.8%, water 79.2%.
In a preferred embodiment, bacillus subtilis active in the step 2 is in the medium
Fermentation time is 30 days, and fermentation temperature is 20-30 DEG C.
In a preferred embodiment, the bulk density that perlite code heap is placed in the step 3 is 150kg/
m3, and selected perlite partial size is 1.2-5mm, weight is 450-600kg/m when saturation absorbs water3。
Technical effect and advantage of the invention:
1, the present invention prevention and treatment orchid diseases cultivation matrix formula it is simple, using bacillus subtilis as bactericidal activity at
Point, the various pathogenic microorganisms for infecting orchid can be effectively prevented；
2, the matrix of present invention prevention and treatment prevention and treatment orchid diseases is easy to use, can be used for the anti-orchid diseases of large area；
3, orchid diseases are prevented using matrix of the present invention, pathogen can be effectively prevent to infect orchid plant；
4, the matrix of present invention prevention and treatment prevention and treatment orchid diseases, is raw material with bacillus subtilis and perlite, effectively avoids
Because chemical pesticide use caused by environmental pollution；
5, in the matrix of present invention prevention and treatment prevention and treatment orchid diseases, bacillus subtilis, which has orchid growth, remarkably promotes work
With.
Specific embodiment
Below in conjunction in the embodiment of the present invention, technical solution in the embodiment of the present invention is carried out clearly and completely
Description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based on this hair
Embodiment in bright, every other implementation obtained by those of ordinary skill in the art without making creative efforts
Example, shall fall within the protection scope of the present invention.
Embodiment 1
The present invention provides a kind of Matrix formulation procedure for preventing and treating orchid diseases, the matrix mainly with bacillus subtilis and
Pearl is made of main component, and specific preparation process includes following operating procedure:
Step 1: biologically active deposit number is selected to make for the bacillus subtilis of ACCC-10619 and perlite
For raw material, and percentage, each component are as follows: biologically active bacillus subtilis 10%, perlite 90%,
It is spare；
Step 2: using bacillus subtilis biologically active in step 1 as bacterium germination strain, and conventional method is used
It is seeded in the culture medium that raw material is beancake powder, corn flour, potassium dihydrogen phosphate and water and is fermented, and by fermentation time control
It is made as 30 days, fermentation temperature control is 20-30 DEG C, and each component by weight hundred in culture medium used in fermentation of bacillus subtilis
Score meter: beancake powder 10%, corn flour 10%, potassium dihydrogen phosphate 0.8%, water 79.2%；
Step 3: carrying out the placement of code heap for the perlite in step 1, be placed in the environment of the ventilation without other miscellaneous bacterias,
Give over to inoculating strain use, wherein the bulk density that perlite code heap is placed is 150kg/m3, and selected perlite partial size is
1.2-5mm, weight is 450-600kg/m when saturation absorbs water3；
Step 4: it after the bacillus subtilis bacterium solution fermented in step 2 filtering, is sprayed on code in step 2 and stacks
On the perlite set, it is placed in natural air drying in the ventilated environment without other miscellaneous bacterias, can be prepared by the matrix of prevention and treatment orchid diseases.
Embodiment 2
The present invention provides a kind of Matrix formulation procedure for preventing and treating orchid diseases, the matrix mainly with bacillus subtilis and
Pearl is made of main component, and specific preparation process includes following operating procedure:
Step 1: biologically active deposit number is selected to make for the bacillus subtilis of ACCC-10619 and perlite
For raw material, and percentage, each component are as follows: biologically active bacillus subtilis 15%, perlite 85%,
It is spare；
Step 2: using bacillus subtilis biologically active in step 1 as bacterium germination strain, and conventional method is used
It is seeded in the culture medium that raw material is beancake powder, corn flour, potassium dihydrogen phosphate and water and is fermented, and by fermentation time control
It is made as 30 days, fermentation temperature control is 20-30 DEG C, and each component by weight hundred in culture medium used in fermentation of bacillus subtilis
Score meter: beancake powder 10%, corn flour 10%, potassium dihydrogen phosphate 0.8%, water 79.2%；
Step 3: carrying out the placement of code heap for the perlite in step 1, be placed in the environment of the ventilation without other miscellaneous bacterias,
Give over to inoculating strain use, wherein the bulk density that perlite code heap is placed is 150kg/m3, and selected perlite partial size is
1.2-5mm, weight is 450-600kg/m when saturation absorbs water3；
Step 4: it after the bacillus subtilis bacterium solution fermented in step 2 filtering, is sprayed on code in step 2 and stacks
On the perlite set, it is placed in natural air drying in the ventilated environment without other miscellaneous bacterias, can be prepared by the matrix of prevention and treatment orchid diseases.
Embodiment 3
The present invention provides a kind of Matrix formulation procedure for preventing and treating orchid diseases, the matrix mainly with bacillus subtilis and
Pearl is made of main component, and specific preparation process includes following operating procedure:
Step 1: biologically active deposit number is selected to make for the bacillus subtilis of ACCC-10619 and perlite
For raw material, and percentage, each component are as follows: biologically active bacillus subtilis 5%, perlite 95%,
It is spare；
Step 2: using bacillus subtilis biologically active in step 1 as bacterium germination strain, and conventional method is used
It is seeded in the culture medium that raw material is beancake powder, corn flour, potassium dihydrogen phosphate and water and is fermented, and by fermentation time control
It is made as 30 days, fermentation temperature control is 20-30 DEG C, and each component by weight hundred in culture medium used in fermentation of bacillus subtilis
Score meter: beancake powder 10%, corn flour 10%, potassium dihydrogen phosphate 0.8%, water 79.2%；
Step 3: carrying out the placement of code heap for the perlite in step 1, be placed in the environment of the ventilation without other miscellaneous bacterias,
Give over to inoculating strain use, wherein the bulk density that perlite code heap is placed is 150kg/m3, and selected perlite partial size is
1.2-5mm, weight is 450-600kg/m when saturation absorbs water3；
Step 4: it after the bacillus subtilis bacterium solution fermented in step 2 filtering, is sprayed on code in step 2 and stacks
On the perlite set, it is placed in natural air drying in the ventilated environment without other miscellaneous bacterias, can be prepared by the matrix of prevention and treatment orchid diseases.
By 3 embodiments, and with existing pesticide control Comparative Examples I :+10% phenyl ether methyl cyclic of 70% thiophanate-methyl suspending agent
Azoles water dispersible granules；Comparative Example II :+42% mancozeb suspending agent of 40% Bravo suspending agent, comparative example are (precious with conventional substrate
It Zhu Yan) cultivates, handles the 30 orchid plant newly transplanted respectively.Experiment is repeated as many times, and the results are shown in Table 1.
1 different substrates of table/medicament is to Major Diseases preventive effect in sword-leaved cymbidium 4
As seen from Table 1, embodiment 1, embodiment 2 and embodiment 3 are all larger than 90% to 4 kinds of Major Diseases preventive effects of orchid, show
It writes and is higher than two groups of comparative examples I and II.
In summary: the present invention is by selecting biologically active deposit number for the bacillus subtilis of ACCC-10619
As bacterium germination strain, and conventional method is used to be seeded to raw material as the culture medium of beancake powder, corn flour, potassium dihydrogen phosphate and water
In ferment, and be 30 days by fermentation time control, fermentation temperature control is 20-30 DEG C, and fermentation of bacillus subtilis institute
Each component percentage in culture medium: beancake powder 10%, corn flour 10%, potassium dihydrogen phosphate 0.8%, water
79.2%, meanwhile, by partial size be 1.2-5mm, perlite carry out the placement of code heap, be placed in the environment of the ventilation without other miscellaneous bacterias
In, give over to inoculating strain use, wherein the bulk density that perlite code heap is placed is 150kg/m3, weight is when saturation absorbs water
450-600kg/m3, after two kinds of raw material preparations are good, after the bacillus subtilis bacterium solution filtering fermented, it is sprayed in step 2
On the perlite that code heap is placed, it is placed in natural air drying in the ventilated environment without other miscellaneous bacterias, can be prepared by prevention and treatment orchid diseases
Matrix, preparation method is simple, easy to use, and can pass through the bacillus subtilis that perlite carries and kill various phytopathies
The original, to achieve the effect that prevent and treat orchid diseases, while active constituent bacillus subtilis used in the present invention belongs to probiotics,
The problems such as capable of avoiding environmental disruption, pathogen drug resistance caused by due to using chemical pesticide, while orchid can also be promoted to plant
Strain growth.
Finally, it should be noted that firstly, relating only to be related to the embodiment of the present disclosure in embodiment disclosed by the invention
Structure, other structures, which can refer to, to be commonly designed, and under not conflict situations, the same embodiment of the present invention and different embodiment can be with
It is combined with each other；
Secondly: the foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, all in the present invention
Spirit and principle within, according to equivalent changes and modifications within the scope of the patent application of the present invention, should all belong to of the invention and cover
Range.
Claims (7)
1. a kind of Matrix formulation procedure for preventing and treating orchid diseases, it is characterised in that: the matrix is mainly with bacillus subtilis and treasure
Pearl is made of main component, and specific preparation process includes following operating procedure:
Step 1: selecting biologically active bacillus subtilis and perlite as raw material, spare；
Step 2: using bacillus subtilis biologically active in step 1 as bacterium germination strain, and using conventional method by its
It is seeded in corresponding culture medium and ferments；
Step 3: the perlite in step 1 is subjected to the placement of code heap, is placed in the environment of the ventilation without other miscellaneous bacterias, gives over to
Inoculating strain is used；
Step 4: after the bacillus subtilis bacterium solution fermented in step 2 filtering, it is sprayed on what code heap in step 2 was placed
On perlite, it is placed in natural air drying in the ventilated environment without other miscellaneous bacterias, can be prepared by the matrix of prevention and treatment orchid diseases.
2. a kind of Matrix formulation procedure for preventing and treating orchid diseases according to claim 1, it is characterised in that: the step 1
In bacillus subtilis be bacillus subtilis that deposit number is ACCC-10619.
3. a kind of Matrix formulation procedure for preventing and treating orchid diseases according to claim 1, it is characterised in that: the step 1
Middle raw material percentage, each component are as follows: biologically active bacillus subtilis 1.00-20.00%, perlite
99-80%.
4. a kind of Matrix formulation procedure for preventing and treating orchid diseases according to claim 1, it is characterised in that: the withered grass bud
Culture medium raw material used in spore bacillus fermentation is beancake powder, corn flour, potassium dihydrogen phosphate and water.
5. a kind of Matrix formulation procedure for preventing and treating orchid diseases according to claim 4, it is characterised in that: the withered grass bud
Each component percentage in culture medium used in spore bacillus fermentation: beancake powder 10%, corn flour 10%, potassium dihydrogen phosphate
0.8%, water 79.2%.
6. a kind of Matrix formulation procedure for preventing and treating orchid diseases according to claim 1, it is characterised in that: the step 2
In the fermentation time of active bacillus subtilis in the medium be 30 days, fermentation temperature is 20-30 DEG C.
7. a kind of Matrix formulation procedure for preventing and treating orchid diseases according to claim 1, it is characterised in that: the step 3
The bulk density that middle perlite code heap is placed is 150kg/m3, and selected perlite partial size is 1.2-5mm, when saturation absorbs water
Weight is 450-600kg/m3。
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Application publication date: 20190827 |
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