CN110150577A - A kind of preparation method and application for dripping chickens' extract - Google Patents
A kind of preparation method and application for dripping chickens' extract Download PDFInfo
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- CN110150577A CN110150577A CN201910410120.1A CN201910410120A CN110150577A CN 110150577 A CN110150577 A CN 110150577A CN 201910410120 A CN201910410120 A CN 201910410120A CN 110150577 A CN110150577 A CN 110150577A
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
- A23L13/30—Meat extracts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
- A23L13/50—Poultry products, e.g. poultry sausages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/10—General methods of cooking foods, e.g. by roasting or frying
- A23L5/13—General methods of cooking foods, e.g. by roasting or frying using water or steam
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a kind of preparation method and application for dripping chickens' extract, this method comprises: taking raw material chicken, after defrosting, cleaning, cutting packing and drying, obtain raw material chicken nugget to be processed；Raw material chicken material to be processed is sealed boiling, boiling temperature is 100~115 DEG C, the time is 8~for 24 hours, obtain drop chickens' extract stoste；After the drop chickens' extract stoste cooling of step (2), it is filtered, filters remaining chicken and chicken bone；It is centrifuged again, isolates grease and a small amount of solid residue, obtain purification drop chickens' extract.Preparation method of the present invention passes through the temperature and time of strict control Sealed steam cooking, significantly improve the content and quality of protein and essential amino acid in drop chickens' extract, and then improve effective nutritional ingredient of drop chickens' extract, the malnutrition of pregnant woman, low birth weight infant and/or growth retardation is significantly improved, and improves the anti-fatigue effect of drop chickens' extract.
Description
Technical field
The present invention relates to drop chickens' extract production technical field more particularly to a kind of preparation method and application for dripping chickens' extract.
Background technique
Low birth weight infant (low birth weight infant, LBWI) is that China pays close attention to crowd.It is most of low
The problems such as nutritious absorption rate of birth weight infants is poor, and allelotaxis is incomplete, mental retardation, therefore increase essence
Refreshing disease, the risk of the illnesss such as infectious diseases.Some researches show that be removed, low birth weight when limiting the factor grown
Youngster's speed of growth will will increase, and this phenomenon is referred to as catch-up growth phenomenon, and the collocation by changing nutrition is advantageously implemented
The catch-up growth of low birth weight infant, enhancing development.Normal diet feeding method cannot fully meet low birth weight
The growth and development demand of youngster needs more nutrients support for low birth weight infant.
It drips chickens' extract and is originated from TaiWan, China, it, will the essence of chicken extracting drop by drop by the way of Steam by water bath drop
Protein in chicken is hydrolyzed to polypeptide.It is high to drip chickens' extract nutritive value, protein and a variety of amino acid rich in needed by human body are
A kind of good infant, pregnant woman and the elderly's nutrient product.
Currently, for the research in terms of drop chicken bouillon product process modification it has been reported that but negligible amounts.
Application publication number discloses a kind of production method for dripping chickens' extract for the application for a patent for invention of CN106722324A, including
Following steps: raw material chicken being placed in enclosed environment and carries out boiling, and controlling boiling temperature is 100~160 DEG C, and cooking pressure is
101.3~618.1KPa, digestion time are 3~6h, and drop chickens' extract is made.
Application publication number discloses a kind of production method for dripping chickens' extract for the application for a patent for invention of CN108925861A, including
Raw material chicken is placed in closed drop extraction bucket, water vapour carries out pressurization vaporization drop extraction, control vaporization drop extraction in drop extraction bucket external heat
Temperature is 105~160 DEG C, and pressure is 1.5~2.5MPa, and the drop extraction time is 3~8 hours, and drop chickens' extract is made.
Although the above method, which has, realizes continuous batch production, improves the yield of drop chickens' extract and improve small molecule ammonia
The advantages that base acid content；But the growth and development demand to really meet low birth weight infant, low birth weight infant is provided
More nutritional supports, then need it is further to the production technology of drop chickens' extract improved, to improve protein and must in drop chickens' extract
Need total lipid content in the content and quality, reduction drop chickens' extract of amino acid.
Summary of the invention
The present invention provides a kind of preparation method and application for dripping chickens' extract, the preparation method is obtained to drip chickens' extract not only albumen
The content and quality of matter and essential amino acid significantly improve, and can improve low birth weight infant immune organ and cranial nerve group
The development knitted alleviates the fatigue of malnutritive pregnant woman, meets the growth and development demand of low birth weight infant, to low birth weight infant
More nutritional supports are provided.
Specific technical solution is as follows:
A kind of preparation method for dripping chickens' extract, comprising the following steps:
(1) raw material chicken is taken, after defrosting, cleaning, cutting packing and drying, obtains raw material chicken nugget to be processed；
(2) raw material chicken material to be processed being sealed boiling, boiling temperature is 100~115 DEG C, the time is 8~for 24 hours,
Obtain drop chickens' extract stoste；
(3) it by after the drop chickens' extract stoste cooling of step (2), is filtered, filters remaining chicken and chicken bone；Carry out again from
The heart isolates grease and a small amount of solid residue, obtains purification drop chickens' extract.
Innovation of the invention is: by the temperature and time of strict control Sealed steam cooking, significantly improving drop chicken
The content and quality of protein and essential amino acid in essence, so improve drop chickens' extract effective nutritional ingredient, to pregnant woman, it is low go out
The malnutrition of raw body weight youngster and/or growth retardation is significantly improved, and improves the antifatigue function of drop chickens' extract
Effect.
Preferably, the boiling temperature is 104~112 DEG C in step (2), the time is 12~20 h.
It is further preferred that the boiling temperature is 108 DEG C, time 16h.
Further, in step (3), the revolving speed of the centrifugation is 1000~1200rpm, and the time is 10~15min.
Most fat can be removed using conventional filtration and the method for centrifugation, but to make to drip total fat in chickens' extract
Content < 0.05%, then need to carry out in-depth filtration.
Further, in step (3), after centrifuge separation, in-depth filtration is also carried out；
The step of in-depth filtration are as follows: the drop chickens' extract solution after centrifuge separation is added to the sample of cleaning deep bed filter
In product ware, deep bed filter valve is opened, the control rate of filtration is 50~100 mL/min, carries out in-depth filtration, is refined
Drip chickens' extract.
The filter material of deep bed filter is made of paper fiber or double-layer filter cloth, tortuous and elongated filtration channel is formed, to drop
The existing surface of fat drop and internal interception function in chickens' extract, and have suction-operated, achieve the effect that almost to completely remove fat.
It further, further include step (4)；
Step (4): after chickens' extract sealed package is dripped in the purification, sterilization treatment is carried out, cools down after sterilizing, is dripped
Chicken bouillon product；
The temperature of the sterilization treatment is 120~125 DEG C, and the time is 10~20min；
The step of cooling are as follows: 15~25 DEG C are cooled in 25~35min.
Sterilization treatment is carried out after sealed package not only ensure that bactericidal effect, but also the drop chickens' extract after sterilization at normal temperature can
Enough long-term preservations and transport, carry with it is edible when it is more convenient.
The present invention provides drip chickens' extract made from a kind of above-mentioned preparation method.
The present invention also provides the drop chickens' extracts to improve pregnant woman, low birth weight infant and/or growth retardation in preparation
Application in the underfed functional food of youngster.
The present invention is had found by mouse experiment, if to the female mice (that is: malnutritive female mice) of daily consumption Low protein diet
Feeding drop chickens' extract, can obviously increase that the weight of female mice, newborn mouse be nascent and postnatal weight, improve newborn mouse immune organ and cranial nerve
The development of tissue；Show that the method for the present invention drop chickens' extract obtained has improvement pregnant woman, low birth weight infant and/or growth and development slow
The slow underfed effect of youngster, can be used for preparing improves pregnant woman, low birth weight infant and/or growth retardation malnutrition
Functional food.
The present invention also provides the drop chickens' extracts to prepare the application in anti-fatigue effect food, in particular in nutrition
Bad pregnant woman.The present invention has found that drop chickens' extract has adult female mice physical fatigue caused by Low protein diet by mouse experiment
Apparent antagonism, being mainly manifested in extends female mice walking weight load significantly, and serum urea nitrogen and lactic acid concn are significant
Decline, it is basic to restore to normal level；It is antifatigue to show that the method for the present invention drop chickens' extract obtained has the function of, can be used for preparing
Antifatigue functional food especially prepares the antifatigue functional food of malnutritive pregnant woman.
Compared with prior art, the invention has the following advantages:
(1) preparation method of the present invention passes through the temperature and time of strict control Sealed steam cooking, significantly improves in drop chickens' extract
The content and quality of protein and essential amino acid, and then effective nutritional ingredient of drop chickens' extract is improved, to pregnant woman, low raw body out
The malnutrition of weight youngster and/or growth retardation is significantly improved, and improves the anti-fatigue effect of drop chickens' extract.
(2) preparation method of the present invention is compared with the traditional method, and reduces boiling temperature, is extended digestion time, is substantially increased
Drip the content and quality of protein and essential amino acid in chickens' extract.
Specific embodiment
The invention will be further described combined with specific embodiments below, and what is be exemplified below is only specific implementation of the invention
Example, but protection scope of the present invention is not limited only to this.
Embodiment 1
A kind of preparation method for dripping chickens' extract, the specific steps are as follows:
(1) it will be put into defrosting pond and thaw by the freezing raw material chicken checked and accepted, until raw material chicken temperature is 4 DEG C；Raw material
It after chicken is put into service sink progress first wash, is cut, is dispensed with 20 kilograms/barrel, carried out in dust proof workshop secondary clear
It washes, after chicken nugget is dried at normal temperature, is put into dry steamer, and seal, outside air is stopped to enter；
(2) steamer being sealed in step (1) is warming up to 104 DEG C, digestion time 12h, obtains drop chickens' extract stoste；
(3) the cold stoste of chickens' extract will be dripped but to room temperature, drop chickens' extract stoste after cooling is taken out from steamer, pours into clean receipts
Collect in bucket, filter remaining chicken and chicken bone using strainer, be then fed into centrifugal device and be centrifuged 15min under 1000rpm, separates
Grease and a small amount of solid residue out, then in-depth filtration is carried out, exquisite drop chickens' extract is made；
The specific steps of in-depth filtration are as follows:
A) deep bed filter is cleaned, it is spare after filter material is clean；
B) the 5L drop chickens' extract solution after taking centrifuge separation, is slowly poured into the planchet of deep bed filter, opens deep layer mistake
Filter valve, the control rate of filtration are 50mL/min, collect filtered exquisite drop chickens' extract；
(4) purification drop chickens' extract is packed into sealed food grade aluminium foil bag after being sufficiently stirred, and after check comprehensively, is sent into bactericidal unit
Middle progress deep layer sterilizing, control sterilization temperature are 121 DEG C, sterilize after 15min, 20 DEG C are cooled in 30min, obtain drop chickens' extract
Product.
Embodiment 2
A kind of preparation method for dripping chickens' extract, the specific steps are as follows:
(1) it will be put into defrosting pond and thaw by the freezing raw material chicken checked and accepted, until raw material chicken temperature is 4 DEG C；Raw material
It after chicken is put into service sink progress first wash, is cut, is dispensed with 20 kilograms/barrel, carried out in dust proof workshop secondary clear
It washes, after chicken nugget is dried at normal temperature, is put into dry steamer, and seal, outside air is stopped to enter；
(2) steamer being sealed in step (1) is warming up to 108 DEG C, digestion time 16h, obtains drop chickens' extract stoste；
(3) the cold stoste of chickens' extract will be dripped but to room temperature, drop chickens' extract stoste after cooling is taken out from steamer, pours into clean receipts
Collect in bucket, filter remaining chicken and chicken bone using strainer, be then fed into centrifugal device and be centrifuged 15min under 1200rpm, separates
Grease and a small amount of solid residue out, then in-depth filtration is carried out, exquisite drop chickens' extract is made；
The specific steps of in-depth filtration are as follows:
A) deep bed filter is cleaned, it is spare after filter material is clean；
B) the 10L drop chickens' extract solution after taking centrifuge separation, is slowly poured into the planchet of deep bed filter, opens deep layer mistake
Filter valve, the control rate of filtration are 100mL/min, collect filtered exquisite drop chickens' extract；
(4) purification drop chickens' extract is packed into sealed food grade aluminium foil bag after being sufficiently stirred, and after check comprehensively, is sent into bactericidal unit
Middle progress deep layer sterilizing, control sterilization temperature are 121 DEG C, sterilize after 15min, 20 DEG C are cooled in 30min, obtain drop chickens' extract
Product.
Embodiment 3
A kind of preparation method for dripping chickens' extract, the specific steps are as follows:
(1) it will be put into defrosting pond and thaw by the freezing raw material chicken checked and accepted, until raw material chicken temperature is 4 DEG C；Raw material
It after chicken is put into service sink progress first wash, is cut, is dispensed with 20 kilograms/barrel, carried out in dust proof workshop secondary clear
It washes, after chicken nugget is dried at normal temperature, is put into dry steamer, and seal, outside air is stopped to enter；
(2) steamer being sealed in step (1) is warming up to 112 DEG C, digestion time 20h, obtains drop chickens' extract stoste；
(3) the cold stoste of chickens' extract will be dripped but to room temperature, drop chickens' extract stoste after cooling is taken out from steamer, pours into clean receipts
Collect in bucket, filter remaining chicken and chicken bone using strainer, be then fed into centrifugal device and be centrifuged 10min under 1200rpm, separates
Grease and a small amount of solid residue out, then in-depth filtration is carried out, exquisite drop chickens' extract is made；
The specific steps of in-depth filtration are as follows:
A) deep bed filter is cleaned, it is spare after filter material is clean；
B) the 10L drop chickens' extract solution after taking centrifuge separation, is slowly poured into the planchet of deep bed filter, opens deep layer mistake
Filter valve, the control rate of filtration are 100mL/min, collect filtered exquisite drop chickens' extract；
(4) purification drop chickens' extract is packed into sealed food grade aluminium foil bag after being sufficiently stirred, and after check comprehensively, is sent into bactericidal unit
Middle progress deep layer sterilizing, control sterilization temperature are 121 DEG C, sterilize after 15min, 20 DEG C are cooled in 30min, obtain drop chickens' extract
Product.
Comparative example 1
A kind of preparation method for dripping chickens' extract, the specific steps are as follows:
(1) it will be put into defrosting pond and thaw by the freezing raw material chicken checked and accepted, until raw material chicken temperature is 4 DEG C；Raw material
It after chicken is put into service sink progress first wash, is cut, is dispensed with 20 kilograms/barrel, carried out in dust proof workshop secondary clear
It washes, after chicken nugget is dried at normal temperature, is put into dry steamer, and seal, outside air is stopped to enter；
(2) steamer being sealed in step (1) is warming up to 120 DEG C, digestion time 6h, obtains drop chickens' extract stoste；
(3) the cold stoste of chickens' extract will be dripped but to room temperature, drop chickens' extract stoste after cooling is taken out from steamer, pours into clean receipts
Collect in bucket, filter remaining chicken and chicken bone using strainer, be then fed into centrifugal device and be centrifuged 15min under 1200rpm, separates
Grease and a small amount of solid residue out, then in-depth filtration is carried out, exquisite drop chickens' extract is made；
The specific steps of in-depth filtration are as follows:
A) deep bed filter is cleaned, it is spare after filter material is clean；
B) the 10L drop chickens' extract solution after taking centrifuge separation, is slowly poured into the planchet of deep bed filter, opens deep layer mistake
Filter valve, the control rate of filtration are 100mL/min, collect filtered exquisite drop chickens' extract；
(4) purification drop chickens' extract is packed into sealed food grade aluminium foil bag after being sufficiently stirred, and after check comprehensively, is sent into bactericidal unit
Middle progress deep layer sterilizing, control sterilization temperature are 121 DEG C, sterilize after 15min, 20 DEG C are cooled in 30min, obtain drop chickens' extract
Product.
Comparative example 2
A kind of preparation method for dripping chickens' extract, the specific steps are as follows:
(1) it will be put into defrosting pond and thaw by the freezing raw material chicken checked and accepted, until raw material chicken temperature is 4 DEG C；Raw material
It after chicken is put into service sink progress first wash, is cut, is dispensed with 20 kilograms/barrel, carried out in dust proof workshop secondary clear
It washes, after chicken nugget is dried at normal temperature, is put into dry steamer, and seal, outside air is stopped to enter；
(2) steamer being sealed in step (1) is warming up to 90 DEG C, digestion time 26h, obtains drop chickens' extract stoste；
(3) the cold stoste of chickens' extract will be dripped but to room temperature, drop chickens' extract stoste after cooling is taken out from steamer, pours into clean receipts
Collect in bucket, filter remaining chicken and chicken bone using strainer, be then fed into centrifugal device and be centrifuged 15min under 1200rpm, separates
Grease and a small amount of solid residue out, then in-depth filtration is carried out, exquisite drop chickens' extract is made；
The specific steps of in-depth filtration are as follows:
A) deep bed filter is cleaned, it is spare after filter material is clean；
B) the 10L drop chickens' extract solution after taking centrifuge separation, is slowly poured into the planchet of deep bed filter, opens deep layer mistake
Filter valve, the control rate of filtration are 100mL/min, collect filtered exquisite drop chickens' extract；
(4) purification drop chickens' extract is packed into sealed food grade aluminium foil bag after being sufficiently stirred, and after check comprehensively, is sent into bactericidal unit
Middle progress deep layer sterilizing, control sterilization temperature are 121 DEG C, sterilize after 15min, 20 DEG C are cooled in 30min, obtain drop chickens' extract
Product.
Embodiment 4
A kind of preparation method for dripping chickens' extract, the specific steps are as follows:
(1) it will be put into defrosting pond and thaw by the freezing raw material chicken checked and accepted, until raw material chicken temperature is 4 DEG C；Raw material
It after chicken is put into service sink progress first wash, is cut, is dispensed with 20 kilograms/barrel, carried out in dust proof workshop secondary clear
It washes, after chicken nugget is dried at normal temperature, is put into dry steamer, and seal, outside air is stopped to enter；
(2) steamer being sealed in step (1) is warming up to 108 DEG C, digestion time 16h, obtains drop chickens' extract stoste；
(3) the cold stoste of chickens' extract will be dripped but to room temperature, drop chickens' extract stoste after cooling is taken out from steamer, pours into clean receipts
Collect in bucket, filter remaining chicken and chicken bone using strainer, is then fed into 1000rpm in centrifugal device and is centrifuged 10min, isolate
Exquisite drop chickens' extract is made in grease and a small amount of solid residue；
(4) purification drop chickens' extract is packed into sealed food grade aluminium foil bag after being sufficiently stirred, and after check comprehensively, is sent into bactericidal unit
Middle progress deep layer sterilizing, control sterilization temperature are 121 DEG C, sterilize after 15min, 20 DEG C are cooled in 30min, obtain drop chickens' extract
Product.
Protein, amino acid and total lipid content are carried out to drop chickens' extract made from Examples 1 to 4 and comparative example 1~2
Measurement, measuring method and result are as follows:
One, test method
(1) protein content determination (Kjeldahl's method)
It weighs drop chickens' extract sample 0.5g to move into dry 100mL Kjeldahl nitrogen determination flask accurately to 0.001g, be added
0.2g copper sulphate and 6g potassium sulfate slightly shake up rear bottleneck and put a small funnel, the 20mL concentrated sulfuric acid be added, by bottle with the oblique Zhi Yu of 45° angle
On foraminate asbestos gauge, using universal electric furnace, the hot digestion in draught cupboard starts with low-temperature heat, complete to content
Portion's charing after foam stops, then increases temperature and keeps slightly boiled, and digestion to liquid continues to heat in after blue-green clear
0.5h is removed and is let cool, and is carefully added 20mL water, after letting cool, is nondestructively transferred in 100mL volumetric flask, and determines nitrogen with a small amount of washing
Bottle, washing lotion are incorporated in volumetric flask, add water to be settled to scale, mixing is spare, as digestive juice.
It checks and washs micro nitrogen testing device.Acid reagent 20mL is measured in triangular flask receiving bottle, mixing instruction is added
Agent 2~3 is dripped, and is inserted into the lower end of condenser pipe under micro nitrogen testing device boric acid liquid level, accurate to draw 10.0mL treatments of the sample liquid,
Reaction chamber is flowed by small funnel, and reaction chamber is flowed into 10mL distillation water washing injection port, rodlike glass plug stoppers.Make 10mL hydrogen-oxygen
Change sodium solution and pour into small glass cup, lifting Bo Sai makes it slowly flow into reaction chamber, is rinsed with a small amount of water immediately by glass corking heavily fortified point, and add
Water, with anti-gas-leak, starts to distill in small glass cup.After being passed through steam transpiration 10min, mobile receiving bottle, liquid level leaves solidifying pipe lower end,
Redistillation 2min.Then it is rinsed outside condenser pipe lower end with a small amount of water, triangular flask is removed, with 0.01mol/L hydrochloric acid standard solution
It is titrated to grey or bluish violet is terminal.Record titration consumption hydrochloric acid standard solution (mL) calculates sample protein matter content.
(2) total lipid content measures
It weighs the drop chickens' extract sample 5g after mixing to be placed in evaporating dish accurately to 0.001g, about 20g quartz sand is added, in
After being evaporated on boiling water bath, in electric drying oven with forced convection after 100 DEG C ± 5 DEG C dry 30min, take out, it is finely ground, all move into filter
In paper tube.Evaporating dish and the glass bar for being stained with sample, are cleaned with the absorbent cotton for speckling with ether, and cotton is put into filtration paper cylinder
It is interior.Filtration paper cylinder is put into the extracting barrel of Soxhlet extractor, connection has been dried to the receiving bottle of constant weight, by extractor condenser pipe
End is added at anhydrous ether or petroleum ether to 2/3rds of bottle internal volume, in heating in water-bath, makes anhydrous ether or petroleum ether
Constantly reflux extracting 10h.At the end of extraction, 1 drop extracting solution is picked up with ground glass stick, is shown on ground glass stick without oil mark
Extraction finishes.It removes receiving bottle, recycles anhydrous ether or petroleum ether, in water-bath when bottle internal solvent residue 1.5mL to be received or so
On be evaporated, then at 100 DEG C ± 5 DEG C dry 1h, weigh after putting in drier cooling 0.5h to constant weight.Calculate total lipid content.
(3) Contents of Amino Acids
The drop chickens' extract sample 5g after mixing is weighed, accurately to 0.001g, is put into hydrolysis pipe, the hydrochloric acid of 6 mol/L is added
15mL is added the phenol 3~4 newly distilled and drips, then hydrolysis pipe is put into refrigerant, freezes 3~5min, then be connected to vacuum pump
On exhaust tube, vacuumize, be then charged with high pure nitrogen, then vacuum nitrogen gas, in triplicate after, in nitrogen charging gaseity lower sealing
Or tighten screw cap and be placed on the hydrolysis pipe sealed in 110 ± 1 DEG C of thermostatic drying chamber, after hydrolysis for 24 hours, take out cooling.It beats
Boiled water solution pipe after filtering hydrolyzate, hydrolysis pipe is repeatedly rinsed with deionized water, hydrolyzate is transferred completely into 50mL volumetric flask
It is interior to use deionized water constant volume.Filtrate 1mL is drawn in 5mL volumetric flask, with vacuum desiccator in 40~50 DEG C of dryings, residue is used
2mL moisture dissolves twice, re-dry, is repeated twice, is finally evaporated, with the buffer solution of 1~2mL pH2.2, for instrument
Device measurement is used.It is accurate to draw 0.200mL kilnitamin standard, it is diluted to 5mL with the buffer of pH 2.2, this standard dilution
Concentration is 5.00nmol/50 μ L, as the amino acid standard of upper machine measurement, with automatic amino acid analyzer with external standard method
The amino acid content of Specimen Determination liquid.
Two, test result
As a result: seeing Tables 1 and 2.
The content of protein, amino acid, total fat in the different embodiments of table 1 or comparative example drop chickens' extract obtained
| Digestion time (h) | Boiling temperature (DEG C) | Protein content (wt%) | Total fat contains (wt%) | |
| Embodiment 1 | 12 | 104 | 5.10 | <0.05 |
| Embodiment 2 | 16 | 108 | 6.70 | <0.05 |
| Embodiment 3 | 20 | 112 | 5.29 | <0.05 |
| Comparative example 1 | 6 | 120 | 2.87 | <0.05 |
| Comparative example 2 | 26 | 90 | 0.75 | <0.05 |
| Embodiment 4 | 16 | 108 | 6.28 | 3.14 |
Amino acid composition and content (g/100g) in the different embodiments of table 2 or comparative example drop chickens' extract obtained
*Essential amino acid
Embodiment 4
1. materials and methods
(1) experimental animal and tested material: 75 SPF grades of healthy adult Female ICR mices, 8 week old, weight are 27 ± 3g；
75 SPF grades of healthy adult male ICR mouses, 6 week old, 25 ± 3g are purchased from this Leco Corp. of Shanghai.Drop chickens' extract is embodiment 1
With product made from comparative example 1, comparative example 2.
(2) feed and reagent: malonaldehyde (MDA), reduced glutathione (GSH), glutathione peroxidase are tested
(GSH-Px), superoxide dismutase (SOD), peroxidase (CAT), lactic acid (LD) testing cassete, urea nitrogen (BUN) reagent
Box (urease method), Bioengineering Research Institute is built up in Nanjing；Standard feed, Low protein diet, one mouse of Changzhou mouse, two company.
(3) animal packet and index determining: Animal adaptability is raised after a week, is chosen 75 female mices, is randomly divided into 5
Group: normal group, model group is dripped chickens' extract group I (product made from embodiment 2), is dripped chickens' extract group II (product made from comparative example 1),
It drips chickens' extract group III (product made from comparative example 2).Normal group gives standard feed, and model group gives Low protein diet, drips chickens' extract
Group gives 8 weeks drop chickens' extracts while giving Low protein diet.All mouse of experimentation are kept illumination in 12 hours, and 12 is small
When it is dark, 21 DEG C -22 DEG C of normal temperature are lived in, in the environment of relative humidity 50%-70%.The body of every group of animal is recorded weekly
Change again.
After experiment carries out 8 weeks, every female mice weight bearing (lead of 10% weight) swimming time is recorded.Each group presses male and female 1:
The mating of 1 ratio, records the average litter size, newborn mouse litter weight at birth, 2 weeks weight of every group of animal.
After newborn mouse 21 days are separated from milk, 5 groups of female mices are subjected to swimming exercise 10 minutes and extract eyeball taking blood, measure cream in serum
Acid and urea nitrogen content.
Newborn mouse after being separated from milk continues to give feed and drop chickens' extract 3 weeks by above-mentioned grouping.The measurement of Morris water maze test is young
Mouse ability of learning and memory.Newborn mouse is put to death, cerebral hippocampal tissue is quickly taken, -80 DEG C of preservations measure oxidative stress parameter and cerebral hippocampal
Bax, bcl-1 expression conditions；Thymus gland, spleen are taken, is weighed.
2. experimental result
The influence for the female mice body weight increase that 2.1 drop chickens' extracts feed Low protein diet
Within entire experimental period, normal group, model group, the average food-intake for dripping chickens' extract group I~III animal, amount of drinking water
Without significant difference.
As shown in table 3, the 1st~3 week, normal group, model group, drip chickens' extract group female mice I~III average weight growth rate without
Significant difference.At the 4th week, the average weight growth rate of chickens' extract group I female mice is dripped compared with model group, significant difference (P < 0.05).
5th~8 week, the average weight growth rate of chickens' extract group I female mice is dripped compared with model group, significant difference (P < 0.01).With model group
It compares, drop chickens' extract group II, III cannot be such that female mice average weight significantly increases.This illustrates the egg in the drop chickens' extract obtained of embodiment 1
The nutritional ingredients such as white matter compensate for the auxotrophy of Low protein diet to a certain extent, play actively to the growth of female mice
Facilitation.
Table 3 drip chickens' extract to female mice body weight growth rate influence (%)
Note: organizing ratio with normal,##P<0.01；With model group ratio,*P < 0.05,**P<0.01。
Influence of the 2.2 drop chickens' extracts to newborn mouse body weight increase
As shown in table 4, model group newborn mouse is averaged litter weight at birth, 2 weeks body weight growth rates, 4 weeks body weight growth rates, 6 weeks weight
Growth rate is substantially less than normal group (P < 0.05).Compared with model group, drop chickens' extract group I newborn mouse is averaged litter weight at birth, Zhou Ti
Weight growth rate dramatically increases (P < 0.05 or P < 0.01).Drop chickens' extract group II, III newborn mouse be averaged litter weight at birth, week body weight increase
Rate is compared with model group, without significant changes.
Table 4 drips influence of the chickens' extract to newborn mouse litter weight at birth and body weight growth rate
Note: organizing ratio with normal,#P < 0.05,##P<0.01；With model group ratio,*P < 0.05,**P<0.01。
Influence of the 2.3 drop chickens' extracts to newborn mouse Development of Immune Organs
As shown in table 5, model group newborn mouse immune organ thymus gland, spleen and weight ratio (thymus index, spleen index) are aobvious
It writes and is lower than normal group (P < 0.01).Compared with model group, drop chickens' extract group I newborn mouse thymus index, spleen index dramatically increase (P <
0.01).Chickens' extract group II, III newborn mouse thymus index, spleen index ratio are dripped compared with model group, without significant changes.This illustrates reality
The nutritional ingredients such as the essential amino acid in the drop chickens' extract obtained of example 1 are applied, facilitation is played to the development of newborn mouse immune organ.
Table 5 drips influence of the chickens' extract to newborn mouse Development of Immune Organs
Note: organizing ratio with normal,##P<0.01；With model group ratio,**P<0.01。
Influence of the 2.4 drop chickens' extracts to newborn mouse ability of learning and memory
As shown in table 6, model group newborn mouse finds water maze plateau time and significantly extends, and the number of shuttle platform is substantially less than
Control group (P < 0.01).Compared with model group, drop chickens' extract group I newborn mouse finds the number of water maze plateau time, shuttle platform
(P < 0.01) is significantly improved, it is horizontal close to normal group.Drop chickens' extract group II, III newborn mouse finds water maze plateau time, shuttles
The number of platform is compared with model group, without significant changes.
Table 6 drips influence of the chickens' extract to newborn mouse ability of learning and memory
Note: organizing ratio with normal,##P<0.01；With model group ratio,**P<0.01。
2.5 drop chickens' extracts to newborn mouse cerebral hippocampal tissue oxidizing stress parameter influence
As shown in table 7, GSH is horizontal compared with normally group newborn mouse, in model group newborn mouse cerebral hippocampal tissue and GSH-Px, CAT,
SOD activity significantly reduces, and the horizontal significant raising (P < 0.01) of MDA illustrates have to newborn mouse nervous system development to Low protein diet
It significantly affects.Compared with model group, drop chickens' extract group I newborn mouse cerebral hippocampal GSH is horizontal and GSH-Px, CAT, SOD activity are significant is risen
Height, MDA level significantly reduce (P < 0.01), horizontal close to normal group.Drip chickens' extract group II, III newborn mouse cerebral hippocampal GSH, MDA
Horizontal and GSH-Px, CAT, SOD activity is compared with model group, without significant changes.
Table 7 drips influence of the chickens' extract to newborn mouse cerebral hippocampal oxidative stress parameter
Note: organizing ratio with normal,##P<0.01；With model group ratio,**P<0.01。
Influence of the 2.6 drop chickens' extracts to newborn mouse cerebral hippocampal tissue bax, bcl-2 gene expression
Bax and bcl-2 albumen is apoptosis regulatory protein family member.The overexpression of bax albumen can accelerate Apoptosis,
And bcl-2 has protective effect to Apoptosis.As shown in table 8, compared with normally group newborn mouse, model group newborn mouse cerebral hippocampal tissue
Middle bax gene relative expression quantity significantly increases, and bcl-2 gene relative expression quantity significantly reduces (P < 0.01), illustrates that low albumen is raised
Material can promote newborn mouse cranial nerve cell apoptosis.Compared with model group, drop chickens' extract group I newborn mouse bax gene relative expression quantity is significant
It reduces, bcl-2 gene relative expression quantity significantly increases (P < 0.01), horizontal close to normal group.Drip chickens' extract group II, III newborn mouse
Bax, bcl-2 gene relative expression quantity are compared with model group in cerebral hippocampal tissue, without significant changes.
Table 8 drips influence of the chickens' extract to newborn mouse cerebral hippocampal tissue bax, bcl-2 gene expression
Note: organizing ratio with normal,##P<0.01；With model group ratio,**P<0.01。
The female mice anti-fatigue effect that 2.7 drop chickens' extracts feed Low protein diet
As shown in table 9, before mating mating, the walking weight load of model group female mice is obviously shortened compared with normal group, is had
There is significant difference (P < 0.01).Drip chickens' extract group I female mice swimming time with model group compared with, with significant difference (P <
0.01) swimming time for, dripping chickens' extract group II, III female mice extends without significant.
As shown in table 10, at the end of entire experiment, after model group female mice power exhausts swimming, serum urea nitrogen and lactic acid concn
It is all remarkably higher than normal group.Compared with model group, drop chickens' extract group I female mice urea in serum nitrogen concentration is significantly reduced with lactic acid
(P < 0.01), it is horizontal close to normal group, these results illustrate the drop chickens' extract obtained of embodiment 2 to caused by Low protein diet at
Year, female mice physical fatigue was with apparent antagonism.Dripping chickens' extract group II, III female mice serum urea nitrogen and lactic acid concn improves not
Significantly.
Table 9 drips influence of the chickens' extract to female mice walking weight load
Note: organizing ratio with normal,##P<0.01；With model group ratio,**P<0.01。
Table 10 drips influence of the chickens' extract to female mice serum urea nitrogen and lactic acid content
Note: organizing ratio with normal,##P<0.01；With model group ratio,**P<0.01。
To sum up, pass through enhancing development test, Development of Immune Organs test, the evaluation of improvement learning and memory, antioxygen
Change test, cranial nerve cell protection test, swimming test and anti-fatigue test etc. research shows that being made by specific process conditions
Drop chickens' extract to caused by Low protein diet adult ICR adult female mice and newborn mouse body weight growth rate decline dramatically increase work
With, ability of learning and memory bad to newborn mouse Development of Immune Organs decline, cerebral hippocampal tissue oxidizing stress, cranial nerve cell apoptosis
Female mice walking weight load is shortened in the effect of being significantly improved, and serum creatinine, urea nitrogen levels raising etc. all have apparent short of money
Anti- effect.This illustrates to drip the nutritional ingredients such as the protein (especially essential amino acid) in chickens' extract, largely compensates for
The auxotrophy of Low protein diet plays positive promotion to the body weight increase of newborn mouse, immune and nervous system development and makees
With.
Above description has shown and described several preferred embodiments of invention, but as previously described, it should be understood that invention is not
It is confined to form disclosed herein, should not be regarded as an exclusion of other examples, and can be used for various other combinations, modification
And environment, and can be carried out within that scope of the inventive concept describe herein by the above teachings or related fields of technology or knowledge
Change.And changes and modifications made by those skilled in the art do not depart from the spirit and scope of invention, then it all should be in the appended power of invention
In the protection scope that benefit requires.
Claims (9)
1. a kind of preparation method for dripping chickens' extract, which comprises the following steps:
(1) raw material chicken is taken, after defrosting, cleaning, cutting packing and drying, obtains raw material chicken nugget to be processed；
(2) raw material chicken material to be processed being sealed boiling, boiling temperature is 100~115 DEG C, the time is 8~for 24 hours, it obtains
Drip chickens' extract stoste；
(3) it by after the drop chickens' extract stoste cooling of step (2), is filtered, filters remaining chicken and chicken bone；It is centrifuged again,
Grease and a small amount of solid residue are isolated, purification drop chickens' extract is obtained.
2. the preparation method of drop chickens' extract as described in claim 1, which is characterized in that in step (2), the boiling temperature is
104~112 DEG C, the time is 12~20h.
3. the preparation method of drop chickens' extract as described in claim 1, which is characterized in that in step (2), the boiling temperature is
108 DEG C, time 16h.
4. the preparation method of drop chickens' extract as described in claim 1, which is characterized in that in step (3), the revolving speed of the centrifugation is
1000~1200rpm, time are 10~15min.
5. as described in claim 1 drop chickens' extract preparation method, which is characterized in that in step (3), after centrifuge separation, also into
Row in-depth filtration；
The step of in-depth filtration are as follows: the drop chickens' extract solution after centrifuge separation is added to the planchet of cleaning deep bed filter
In, deep bed filter valve is opened, the control rate of filtration is 50~100mL/min, carries out in-depth filtration, obtains purification drop chicken
Essence.
6. the preparation method of drop chickens' extract as described in claim 1, which is characterized in that further include step (4)；
Step (4): after chickens' extract sealed package is dripped in the purification, carrying out sterilization treatment, cool down after sterilizing, obtains drop chickens' extract
Product；
The temperature of the sterilization treatment is 120~125 DEG C, and the time is 10~20min；
The step of cooling are as follows: 15~25 DEG C are cooled in 25~35min.
7. a kind of preparation method as described in any one of claims 1 to 6 is obtained to drip chickens' extract.
8. drop chickens' extract improves pregnant woman, low birth weight infant and/or growth retardation battalion in preparation as claimed in claim 7
Support the application in undesirable functional food.
9. drop chickens' extract is preparing the application in anti-fatigue effect food as claimed in claim 7.
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| CN115944049A (en) * | 2022-12-28 | 2023-04-11 | 抚州民生农业科技有限公司 | Production method and production equipment for extracting chicken essence stock solution |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101043825A (en) * | 2004-01-30 | 2007-09-26 | 康尼格拉食品及食品配料有限公司 | Process for producing a low fat, concentrated meat from meat by-products |
| CN103315363A (en) * | 2013-05-21 | 2013-09-25 | 天津科技大学 | Seabuckthorn fruit normal juice degreasing processing method |
| CN105986520A (en) * | 2016-01-29 | 2016-10-05 | 安徽凤凰滤清器股份有限公司 | Mixed filler added filter paper for hydraulic filter |
| CN106722324A (en) * | 2017-01-12 | 2017-05-31 | 曹成刚 | A kind of production method and production equipment for dripping chickens' extract |
| CN206549312U (en) * | 2017-03-11 | 2017-10-13 | 昆山市华滤环保科技有限公司 | A kind of Novel super-thin fiber high precision filter bag |
| CN107970680A (en) * | 2017-11-30 | 2018-05-01 | 武汉钢铁有限公司 | A kind of paper tape formula ungrease treatment filtration system and method with precoating function |
| CN108925861A (en) * | 2018-07-20 | 2018-12-04 | 史家怡 | A kind of production method for dripping chickens' extract |
-
2019
- 2019-05-17 CN CN201910410120.1A patent/CN110150577A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101043825A (en) * | 2004-01-30 | 2007-09-26 | 康尼格拉食品及食品配料有限公司 | Process for producing a low fat, concentrated meat from meat by-products |
| CN103315363A (en) * | 2013-05-21 | 2013-09-25 | 天津科技大学 | Seabuckthorn fruit normal juice degreasing processing method |
| CN105986520A (en) * | 2016-01-29 | 2016-10-05 | 安徽凤凰滤清器股份有限公司 | Mixed filler added filter paper for hydraulic filter |
| CN106722324A (en) * | 2017-01-12 | 2017-05-31 | 曹成刚 | A kind of production method and production equipment for dripping chickens' extract |
| CN206549312U (en) * | 2017-03-11 | 2017-10-13 | 昆山市华滤环保科技有限公司 | A kind of Novel super-thin fiber high precision filter bag |
| CN107970680A (en) * | 2017-11-30 | 2018-05-01 | 武汉钢铁有限公司 | A kind of paper tape formula ungrease treatment filtration system and method with precoating function |
| CN108925861A (en) * | 2018-07-20 | 2018-12-04 | 史家怡 | A kind of production method for dripping chickens' extract |
Non-Patent Citations (1)
| Title |
|---|
| 张迪钧: "探讨国产寡产鸡制成之滴鸡精对不同年龄(青少年期、壮年期、老年期)及性别之小鼠抗疲劳之效果", 《HTTPS://JOUR.DUXIU.COM/THESISDETAIL.JSP?DXNUMBER=390107990222&D=B00DD1FF04D18E6E04511A78A4C52CAC》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115944049A (en) * | 2022-12-28 | 2023-04-11 | 抚州民生农业科技有限公司 | Production method and production equipment for extracting chicken essence stock solution |
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