CN110122183A - A kind of uninterrupted fruiting matrix of non fast growing tree species hayashishita multi items and application - Google Patents
A kind of uninterrupted fruiting matrix of non fast growing tree species hayashishita multi items and application Download PDFInfo
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- CN110122183A CN110122183A CN201910434094.6A CN201910434094A CN110122183A CN 110122183 A CN110122183 A CN 110122183A CN 201910434094 A CN201910434094 A CN 201910434094A CN 110122183 A CN110122183 A CN 110122183A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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Abstract
The present invention relates to a kind of uninterrupted fruiting matrix of non fast growing tree species hayashishita multi items and application, the uninterrupted fruiting matrix component is as follows: corn core bit, broad-leaved wood chip, beanstalk bits, wheat bran, pulverized limestone, land plaster, water-retaining agent.The present invention is passed through using the hayashishita region of non fast growing tree species to mushroom, it picks up the ears, coprinus comatus, ganoderma lucidum four large-scale kinds proportionally carry out hayashishita cultivation using specific cultivation matrix, it realizes after hayashishita regional temperature reaches 5 DEG C or more, the technical effect of uninterrupted fruiting, each kind competitively occurs, color is different, it comes in every shape, head is different, it is confused with errors and omission, time of origin and the difference for growing up to the time are larger, considerably increase the enjoyment of tourism, the enjoyment of picking, for developing bacterium industry tourism, develop forest economy, sufficiently excavate hayashishita resource, accelerate Promoting Industrial structural adjustment, be conducive to remote mountain areas hayashishita industry development and accurate poverty alleviation.
Description
Technical field
The present invention relates to a kind of uninterrupted fruiting matrix of non fast growing tree species hayashishita multi items and applications, belong to edible fungus culturing
Technical field.
Background technique
Since last century the fifties, the whole people of the generation in the big ground of motherland afforest, improve the activities such as afforestation rate
Carried out at normality always, the tree species of such greening, especially height above sea level is higher, on the opposite thin mountainous region of ridge, mostly arbor-vitae, masson pine
The deciduous species such as the needles such as (or black pine), Chinese pine, larch and locust tree, toothed oak wood, relative to poplar, willow, Chinese wax, method paulownia,
For paulownia, such is non fast growing tree species, especially in the mountainous region that naked rock ratio is high, soil layer is partially thin, even more needs decades
Growth period can be only achieved certain canopy density.
Since 2008, scientific research personnel was dedicated to hayashishita cultivation technical research, focused primarily upon middle High aititude hayashishita list
The cultivation of kind, such as pick up the ears, mushroom, agaric, can cultivation in flakes, convenient for concentrating harvesting and managing, scientific research personnel is referred to as
For " hubbed mode ", this kind of hayashishita cultivation mode gradually exposes some problems in exploitation, for example the cultivation phase concentrates, and as a result must
It is so to concentrate picking time, is again difficult to find mushroom in a period of time after harvesting, especially does the production of bacterium industry tourism, largely enable
Visitor has the worry of a kind of " taking a chance ", loses confidence unavoidably to the tourist spot, this one；Second, concentrating the product after harvesting
Urgent need handled or it is fresh sell or process, or even occur rotten mushroom phenomenon sometimes, very unfortunately；Third, single variety centralized production
The employment of management is very concentrated, and busy, which is worked extra shifts or extra hours, catches up with to come over yet, and fruiting intermittent phase administrative staff are idle, very not busy
It dissipates, is equally unfavorable for managing；4th, in the fruiting intermittent phase, because no mushroom can be adopted, visitor and administrative staff no longer go up a hill, and make forest zone
Fire prevention reenter dangerous periods.In short, hubbed mode itself is difficult to the drawbacks of overcoming, scientific research personnel is had been devoted to
Seek solution.
It is eaten under a kind of natural forests as Chinese patent literature CN106069207A (application number 201610708160.0) is disclosed
With bacterium composite ecological cultivation method, the selection including natural forest land, planting season is arranged, and edible fungus culturing, daily management and is adopted
Pluck step.Edible fungi complex ecological cultivation is novel forest economy mode under natural forests, and forest and edible mushroom not only grow sky
Between it is complementary, the factors complementation such as Er Qieguang, heat, water, gas, soil is reciprocal, circulation gain, cooperative development, forms a kind of compound ecology
Development model.The contradiction of forestry long-acting economy and peasant's short-term yield can be solved, it is economical, ecological all good with social benefit, to woods
Industry sustainable development and agriculture, rural areas and farmers Resolving probiems are of great significance.The present invention utilizes hayashishita natural conditions culturing edible fungus, in conjunction with
Tourism picking provides composite cultivation mode under the brand-new natural forests of one kind.But the planting type still has cultivation strain list
One, the problems such as harvest management time concentrates.
Summary of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of uninterrupted fruiting matrix of non fast growing tree species hayashishita multi items and
Using.
Technical solution of the present invention is as follows:
A kind of uninterrupted fruiting matrix of non fast growing tree species hayashishita multi items, it is parts by weight that component is as follows:
180~220 parts of corn core bit, 70~90 parts of broad-leaved wood chip, 70~90 parts of beanstalk bits, 35~45 parts of wheat bran, lime
7~9 parts of powder, 3.5~4.5 parts of land plaster, 0.8~1.2 part of water-retaining agent.
Preferred according to the present invention, it is parts by weight that component is as follows:
200 parts of corn core bit, 80 parts of broad-leaved wood chip, beanstalk considers 80 parts to be worth doing, 40 parts of wheat bran, 8 parts of pulverized limestone, 4 parts of land plaster, protects
1 part of aqua.
Preferred according to the present invention, the partial size of the corncob and soya bean stem meal is not more than 10mm；The grain of the broad-leaved wood chip
Diameter is not more than 5mm.
It is preferred according to the present invention, water-retaining agent water absorbent rate >=120；
Preferred according to the present invention, the moisture content of the matrix is 57%~63%.
Preferred according to the present invention, the pH value of the matrix is 6.5~7.5；The pH adjusting agent of matrix is using quality hundred
Divide the acetic acid that specific concentration is 10% or the limewash that mass percent concentration is 5%.
The preparation method of above-mentioned matrix, steps are as follows:
(1) broad-leaved wood chip is mixed with part pulverized limestone, then in material water quality ratio 1:(0.5~0.7) ratio stir
Uniformly, premix sawdust is made in air storage 6~8 days；
(2) corn core bit and beanstalk bits are mixed with remaining pulverized limestone, then by material water quality ratio 1:(1.0~1.4)
Ratio stir evenly, air storage 4~6 days, be made premix core material；
(3) wheat bran, land plaster are mixed thoroughly jointly, auxiliary material mixture is made；
(4) by water-retaining agent and water 1:(180~220 in mass ratio) ratio mixing, stand 1~2 hour, then with step
(1) the agitated mixing of auxiliary material mixture made from premix sawdust obtained, step (2) premix core material obtained and step (3), i.e.,
?.
Preferred according to the present invention, in the step (1), pulverized limestone accounts for the 50% of pulverized limestone gross mass.
It is preferred according to the present invention, in the step (1), expect water quality ratio 1:0.6, air storage 7 days.
It is preferred according to the present invention, in the step (2), expect water quality ratio 1:1.2, air storage 5 days.
Preferred according to the present invention, in the step (3), the ratio of water-retaining agent and water 1:200 in mass ratio are mixed.
The application of the above-mentioned uninterrupted fruiting matrix of non fast growing tree species hayashishita multi items, steps are as follows:
(i) the uninterrupted fruiting matrix of above-mentioned non fast growing tree species hayashishita multi items is packed, is then sterilized, bacteria stick is made；
(ii) mushroom strain is accessed into bacteria stick made from step (i), the bacterium germination 125~145 days under the conditions of 5~20 DEG C,
Alternatively, bacterium germination 80~90 days under the conditions of 21~28 DEG C；Then, temperature adjustment is to 15~25 DEG C, relative air humidity 70~80%, light
It according to 700~900lx of intensity, gravity-flow ventilation, carries out annesl 20~30 days, lentinus edodes strain stick is made；
(iii) into bacteria stick made from step (i), access is picked up the ears strain, the bacterium germination 30~40 days under the conditions of 5~20 DEG C, or
Person, the bacterium germination 12~18 days under the conditions of 21~28 DEG C；Then, it under the conditions of 0~4 DEG C, carries out mycelia After-mature cultivation 18~22 days,
Bacteria stick of picking up the ears is made；
(iv) strain of coprinus comatus is accessed into bacteria stick made from step (i), the bacterium germination 25~35 days under the conditions of 5~20 DEG C,
Alternatively, bacterium germination 12~18 days under the conditions of 21~28 DEG C；Then, under the conditions of 0~4 DEG C, mycelia After-mature cultivation 18~22 is carried out
It, is made coprinus comatus bacteria stick；
(v) lucidum strain is accessed into bacteria stick made from step (i), the bacterium germination 35~45 days under the conditions of 5~20 DEG C, or
Person, the bacterium germination 22~28 days under the conditions of 21~28 DEG C；Then, it under the conditions of 0~4 DEG C, carries out mycelia After-mature cultivation 18~22 days,
Ganoderma lucidum bacteria stick is made；
(vi) by chicken made from lentinus edodes strain stick made from step (ii), step (iii) bacteria stick obtained of picking up the ears, step (iv)
Ganoderma lucidum bacteria stick made from leg mushroom stick and step (v) activates 12~48 hours under normal temperature conditions, then according to lentinus edodes strain stick: side
Ear bacteria stick: coprinus comatus bacteria stick: the ratio that ganoderma lucidum bacteria stick quantity ratio is 5: 10: 4: 4 is embedded in the canopy density that area is not less than 1000 mu
Thickness of earth covering is 2~5cm on 0.2~0.7 non fast growing forestland, bacteria stick, and 0.3~0.5 meter of cultivation cave interval carries out
Management of producing mushroom.
Preferred according to the present invention, in the step (i), the specification of bacteria stick is to prepare the folding diameter of the double-layer plastic bag of bacteria stick
The flat width x length degree of specification indicates: lentinus edodes strain stick (20~24) cm × (50~60) cm, bacteria stick of picking up the ears (20~24) 22cm × (40~
50) cm, coprinus comatus bacteria stick (15~20) cm × (25~35) cm, ganoderma lucidum bacteria stick (15~20) cm × (25~35) cm.
It is preferred according to the present invention, in the step (ii), mushroom (Lentinus edodes) selected from high temperature type, in
Wide warm type and/or middle low form kind；It is further preferred that the product name of the high temperature type kind is " agriculture perfume (or spice) 69 " bacterium
Strain, it is described in the product name of wide warm type kind be " agriculture perfume (or spice) 66 " bacterial strain, the product name of the middle low form kind is " Shandong flower
No.1 " bacterial strain.
It is preferred according to the present invention, in the step (iii), pick up the ears selected from high temperature modification kind, low form kind and/or in
Wide warm type kind；It is further preferred that the high temperature modification kind is branched oyster mushroom (P.cornucopiae) bacterial strain, the low temperature
Type kind is oyster cap fungus (Pleuotus ostreatus) bacterial strain, it is described in wide warm type kind be pleurotus florida
(P.florida) bacterial strain；
Preferred according to the present invention, in the step (iv), coprinus comatus (Coprinus comatus) is selected from coprinus comatus
Cc258 and/or long leg coprinus comatus；
Preferred according to the present invention, in the step (v), ganoderma lucidum is selected from Ganoderma lucidum (Ganoderma lucidum
(Leyss.exFr.) Karst.) bacterial strain, Han Zhi (Ganoderma lucidum) bacterial strain, black sesame (Fructificatio
Amaurodermatis Rudae) bacterial strain and/or green sesame (Coriolus versicolar (L.:Fr.) Quel.) bacterial strain；
It is preferred according to the present invention, in the step (vi), 0.5~1, each cultivation cave bacteria stick.0.5 bacteria stick is will
After bacteria stick peels plastic film off, is cut from centre, make into two small stubs, one of them is 0.5 bacteria stick.
Preferred according to the present invention, in the step (vi), the canopy density of non fast growing forestland are 0.4~0.7.
Beneficial effect
1, the present invention using non fast growing tree species hayashishita region by mushroom, pick up the ears, coprinus comatus, the large-scale product of ganoderma lucidum four
Kind hayashishita cultivation is proportionally carried out using specific cultivation matrix, realized after hayashishita regional temperature reaches 5 DEG C or more, not between
The technical effect of disconnected fruiting, each kind competitively occur, and color is different, come in every shape, head is different, confused with errors and omission, time of origin
It is larger with the difference that grows up to the time, the enjoyment of tourism, the enjoyment of picking are considerably increased, for the tourism of developing bacterium industry, develops woods
Lower economy, sufficiently excavate hayashishita resource, accelerate Promoting Industrial structural adjustment, be conducive to remote mountain areas hayashishita industry development and precisely
Poverty alleviation；
2, it since invention fruiting speed and fruiting amount are more uniform, thus avoids existing single variety fruiting and concentrates largely
Fruiting causes the pressure of picking, transport etc., especially non fast growing woods to be typically in remote mountain areas, concentrates picking, processing, fortune
Defeated condition is poor, and which is conducive to significantly improve the income of peasant household, reduces the Planting risk of peasant household；
3, due to the present invention using mushroom, pick up the ears, coprinus comatus, the large-scale kinds of ganoderma lucidum four, except temperature is special lower than 5 DEG C
Outside period, remaining time can have mushroom that can see, have mushroom that can adopt, and significantly increase the fruiting time in forest land, effectively reduce fruiting
The neutral gear phase, not only improve subsequent management of producing mushroom, and increase visitor to the enthusiasm on the ground.
Detailed description of the invention
Fig. 1 is that 5 hayashishita of embodiment picks up the ears to concentrate the photo after fruiting；
Fig. 2 is that 5 hayashishita mushroom of embodiment concentrates the photo after fruiting；
Specific embodiment
Below with reference to embodiment, technical scheme is described further, but institute's protection scope of the present invention is not limited to
This.
Strain source
Mushroom (Lentinus edodes) kind " agriculture perfume (or spice) 69 " used in the examples, " agriculture perfume (or spice) 66 ", " Shandong flower No.1 " are
For ordinary commercial products, Shandong Academy of Agricultural Science, Institute of Agricultural Resources and Environment is on sale；
Branched oyster mushroom (P.cornucopiae) bacterial strain used in the examples, oyster cap fungus (Pleuotus
Ostreatus) bacterial strain, pleurotus florida (P.florida) bacterial strain are ordinary commercial products, Shandong Academy of Agricultural Sciences's agriculture
Industry resource and environment research institute is on sale；
Coprinus comatus (Coprinus comatus) kind coprinus comatus Cc258 used in the examples, long leg coprinus comatus are
Ordinary commercial products, Shandong Academy of Agricultural Science, Institute of Agricultural Resources and Environment are on sale；
Ganoderma lucidum (Ganoderma lucidum (Leyss.exFr.) Karst.) bacterial strain, Korea Spro used in the examples
Sesame (Ganoderma lucidum) bacterial strain, black sesame (Fructificatio Amaurodermatis Rudae) bacterial strain, green sesame
(Coriolus versicolar (L.:Fr.) Quel.) bacterial strain is ordinary commercial products, Shandong Academy of Agricultural Sciences's agricultural money
Source is on sale with Environmental Research Institute；
Reagent
Water-retaining agent used in the examples is to inhale the ordinary commercial products that pure water multiplying power is 120, Shandong Province Yanggu County dragon's fountain
Chemical plant is on sale.
Embodiment 1
A kind of uninterrupted fruiting matrix of non fast growing tree species hayashishita multi items, it is parts by weight that component is as follows:
200 parts of corn core bit, 80 parts of broad-leaved wood chip, beanstalk considers 80 parts to be worth doing, 40 parts of wheat bran, 8 parts of pulverized limestone, 4 parts of land plaster, protects
1 part of aqua；
The partial size of the corncob and soya bean stem meal is not more than 10mm；The partial size of the broad-leaved wood chip is not more than 5mm；
The moisture content of the matrix is 60%；The pH value of matrix is 7, and the pH adjusting agent of matrix is that mass percent concentration is
The limewash that 10% acetic acid or mass percent concentration is 5%.
The preparation method of above-mentioned matrix, steps are as follows:
(1) broad-leaved wood chip is mixed with the pulverized limestone of 50% weight, is then stirred in the ratio of material water quality ratio 1:0.6 equal
It is even, air storage 7 days, premix sawdust is made；
(2) corn core bit and beanstalk bits are mixed with remaining pulverized limestone, is then stirred in the ratio of material water quality ratio 1:1.2
It mixes uniformly, air storage 5 days, premix core material is made；
(3) wheat bran, land plaster are mixed thoroughly jointly, auxiliary material mixture is made；
(4) ratio of water-retaining agent and water 1:200 in mass ratio are mixed, stands 1.5 hours, be then made with step (1)
Premix sawdust, the agitated mixing of auxiliary material mixture made from step (2) premix core material obtained and step (3) to get.
Embodiment 2
A kind of uninterrupted fruiting matrix of non fast growing tree species hayashishita multi items, it is parts by weight that component is as follows:
180 parts of corn core bit, 90 parts of broad-leaved wood chip, 70 parts of beanstalk bits, 45 parts of wheat bran, 7 parts of pulverized limestone, 4.5 parts of land plaster,
0.8 part of water-retaining agent.
The partial size of the corncob and soya bean stem meal is not more than 10mm；The partial size of the broad-leaved wood chip is not more than 5mm；
The moisture content of the matrix is 57%；The pH value of matrix is 7.5, and the pH adjusting agent of matrix is mass percent concentration
The limewash that acetic acid or mass percent concentration for 10% are 5%.
The preparation method of above-mentioned matrix, steps are as follows:
(1) broad-leaved wood chip is mixed with the pulverized limestone of 50% weight, is then stirred in the ratio of material water quality ratio 1:0.5 equal
It is even, air storage 8 days, premix sawdust is made；
(2) corn core bit and beanstalk bits are mixed with remaining pulverized limestone, is then stirred in the ratio of material water quality ratio 1:1.0
It mixes uniformly, air storage 6 days, premix core material is made；
(3) wheat bran, land plaster are mixed thoroughly jointly, auxiliary material mixture is made；
(4) ratio of water-retaining agent and water 1:180 in mass ratio are mixed, stands 2 hours, it is then obtained with step (1)
Premix the agitated mixing of auxiliary material mixture made from sawdust, step (2) premix core material obtained and step (3) to get.
Embodiment 3
A kind of uninterrupted fruiting matrix of non fast growing tree species hayashishita multi items, it is parts by weight that component is as follows:
220 parts of corn core bit, 70 parts of broad-leaved wood chip, 90 parts of beanstalk bits, 35 parts of wheat bran, 9 parts of pulverized limestone, 3.5 parts of land plaster,
1.2 parts of water-retaining agent.
The partial size of the corncob and soya bean stem meal is not more than 10mm；The partial size of the broad-leaved wood chip is not more than 5mm；
The moisture content of the matrix is 63%；The pH value of matrix is 6.5, and the pH adjusting agent of matrix is mass percent concentration
The limewash that acetic acid or mass percent concentration for 10% are 5%.
The preparation method of above-mentioned matrix, steps are as follows:
(1) broad-leaved wood chip is mixed with the pulverized limestone of 50% weight, is then stirred in the ratio of material water quality ratio 1:0.7 equal
It is even, air storage 6 days, premix sawdust is made；
(2) corn core bit and beanstalk bits are mixed with remaining pulverized limestone, is then stirred in the ratio of material water quality ratio 1:1.4
It mixes uniformly, air storage 4 days, premix core material is made；
(3) wheat bran, land plaster are mixed thoroughly jointly, auxiliary material mixture is made；
(4) ratio of water-retaining agent and water 1:220 in mass ratio are mixed, stands 1 hour, it is then obtained with step (1)
Premix the agitated mixing of auxiliary material mixture made from sawdust, step (2) premix core material obtained and step (3) to get.
Comparative example 1
The uninterrupted fruiting matrix of non fast growing tree species hayashishita multi items as described in Example 1, the difference is that, by broad-leaved
Sawdust such as replaces at the beanstalk bits of weight.
The preparation method is the same as that of Example 1.
Comparative example 2
The uninterrupted fruiting matrix of non fast growing tree species hayashishita multi items as described in Example 1, the difference is that, by corn
Core bits such as replace at the broad-leaved wood chip of weight.
The preparation method is the same as that of Example 1.
Embodiment 4
A kind of method of the uninterrupted fruiting of non fast growing tree species hayashishita multi items, steps are as follows:
(i) matrix described in embodiment 1-3 and comparative example 1-2 is packed respectively respectively, is respectively labeled as experimental group 1-5, so
After sterilize, be made bacteria stick；The specification of bacteria stick is to prepare the flat width x length degree table of folding diameter specification of folding bottom (bilayer) polybag of bacteria stick
Show:
Lentinus edodes strain stick 22cm × 55cm, bacteria stick of picking up the ears 22cm × 45cm, coprinus comatus bacteria stick 18cm × 30cm, ganoderma lucidum bacteria stick
18cm×30cm；
(ii) mushroom strain is accessed into bacteria stick made from step (i), the bacterium germination 135 days under the conditions of 12 DEG C, then, temperature adjustment
It to 20 DEG C, relative air humidity 75%, intensity of illumination 800lx, gravity-flow ventilation, carries out annesl 12 days, lentinus edodes strain stick is made；
Mushroom (Lentinus edodes) is selected from " agriculture perfume (or spice) 69 " bacterial strain, " agriculture perfume (or spice) 66 " bacterial strain, " Shandong flower No.1 " bacterial strain and respectively connects
Kind one third lentinus edodes strain stick；
(iii) strain of picking up the ears, the bacterium germination 35 days under the conditions of 12 DEG C, then, 0 are accessed into bacteria stick made from step (i)
It under the conditions of DEG C, carries out mycelia After-mature cultivation 20 days, bacteria stick of picking up the ears is made；
Pick up the ears selected from branched oyster mushroom (P.cornucopiae) bacterial strain, oyster cap fungus (Pleuotus ostreatus) bacterial strain,
Pleurotus florida (P.florida) bacterial strain is respectively inoculated with one third and picks up the ears bacteria stick；
(iv) strain of coprinus comatus is accessed into bacteria stick made from step (i), the bacterium germination 30 days under the conditions of 12 DEG C, then, 0
It under the conditions of DEG C, carries out mycelia After-mature cultivation 20 days, coprinus comatus bacteria stick is made；
Coprinus comatus (Coprinus comatus) is selected from coprinus comatus Cc258 bacterial strain, long leg coprinus comatus strain；Wherein long leg chicken
Leg mushroom bacterial strain is inoculated with 2/3rds coprinus comatus bacteria sticks, coprinus comatus Cc258 strain inoculated one third coprinus comatus bacteria stick；
(v) lucidum strain is accessed into bacteria stick made from step (i), the bacterium germination 40 days under the conditions of 12 DEG C, alternatively, at 25 DEG C
Under the conditions of bacterium germination 25 days；Then, it under the conditions of 0 DEG C, carries out mycelia After-mature cultivation 20 days, ganoderma lucidum bacteria stick is made；
Ganoderma lucidum is selected from Ganoderma lucidum (Ganoderma lucidum (Leyss.exFr.) Karst.) bacterial strain, Han Zhi
(Ganoderma lucidum) bacterial strain, black sesame (Fructificatio Amaurodermatis Rudae) bacterial strain, green sesame
(Coriolus versicolar (L.:Fr.) Quel.) bacterial strain is respectively inoculated with a quarter ganoderma lucidum bacteria stick；
(vi) by chicken made from lentinus edodes strain stick made from step (ii), step (iii) bacteria stick obtained of picking up the ears, step (iv)
Ganoderma lucidum bacteria stick made from leg mushroom stick and step (v) activates 12~48 hours under normal temperature conditions, then according to lentinus edodes strain stick: side
Ear bacteria stick: coprinus comatus bacteria stick: the ratio that ganoderma lucidum bacteria stick quantity ratio is 5: 10: 4: 4 is embedded in 1000 mu of area of canopy density 0.5
Non fast growing forestland, thickness of earth covering is 4~5cm, 0.4~0.5 meter of cultivation cave interval, 1, each cultivation cave bacterium above bacteria stick
Stick carries out management of producing mushroom.
Through counting, various edible fungus biology efficiency are as shown in table 1:
Table 1
| Group | Mushroom biological efficiency | It picks up the ears biological efficiency | Coprinus comatus biological efficiency | Ganoderma lucidum biological efficiency (dry product meter) |
| Experimental group 1 | 160.1% | 190.3% | 140.3% | 15.3% |
| Experimental group 2 | 150.6% | 175.2% | 132.1% | 13.6% |
| Experimental group 3 | 151.3% | 174.7% | 128.9% | 13.4% |
| Experimental group 4 | 109.8% | 128.9% | 133.2% | 11.8% |
| Experimental group 5 | 150.1% | 170.5% | 101.3% | 13.2% |
Embodiment 5
A kind of method of the uninterrupted fruiting of non fast growing tree species hayashishita multi items, steps are as follows:
(i) matrix described in embodiment 1-3 and comparative example 1-2 is packed respectively respectively, is respectively labeled as experimental group 1-5, so
After sterilize, be made bacteria stick；The specification of bacteria stick is to prepare the flat width x length degree table of folding diameter specification of folding bottom (bilayer) polybag of bacteria stick
Show:
Lentinus edodes strain stick 22cm × 55cm, bacteria stick of picking up the ears 22cm × 45cm, coprinus comatus bacteria stick 18cm × 30cm, ganoderma lucidum bacteria stick
18cm×30cm；
(ii) mushroom strain is accessed into bacteria stick made from step (i), the bacterium germination 85 days under the conditions of 25 DEG C；Then, temperature adjustment
It to 20 DEG C, relative air humidity 70%, intensity of illumination 900lx, gravity-flow ventilation, carries out annesl 10 days, lentinus edodes strain stick is made；
Mushroom (Lentinus edodes) is selected from " agriculture perfume (or spice) 69 " bacterial strain；
(iii) strain of picking up the ears, the bacterium germination 15 days under the conditions of 25 DEG C are accessed into bacteria stick made from step (i)；Then, 4
It under the conditions of DEG C, carries out mycelia After-mature cultivation 18 days, bacteria stick of picking up the ears is made；
It picks up the ears selected from branched oyster mushroom (P.cornucopiae) bacterial strain；
(iv) strain of coprinus comatus is accessed into bacteria stick made from step (i), the bacterium germination 18 days under the conditions of 21 DEG C；Then, 4
It under the conditions of DEG C, carries out mycelia After-mature cultivation 22 days, coprinus comatus bacteria stick is made；
Coprinus comatus (Coprinus comatus) is selected from long leg coprinus comatus strain；
(v) lucidum strain is accessed into bacteria stick made from step (i), the bacterium germination 22 days under the conditions of 28 DEG C；Then, at 0 DEG C
Under the conditions of, it carries out mycelia After-mature cultivation 22 days, ganoderma lucidum bacteria stick is made；
Ganoderma lucidum is selected from Ganoderma lucidum (Ganoderma lucidum (Leyss.exFr.) Karst.) bacterial strain；
(vi) by chicken made from lentinus edodes strain stick made from step (ii), step (iii) bacteria stick obtained of picking up the ears, step (iv)
Ganoderma lucidum bacteria stick made from leg mushroom stick and step (v) activates 48 hours under normal temperature conditions, then according to lentinus edodes strain stick: Pleurotus sp2
Stick: coprinus comatus bacteria stick: the ratio that ganoderma lucidum bacteria stick quantity ratio is 5: 10: 4: 4 is embedded in non-speed of 1500 mu of area of the canopy density 0.7
Raw forestland, thickness of earth covering is 2~3cm above bacteria stick, 0.3~0.4 meter of cultivation cave interval, 0.5, each cultivation cave bacteria stick,
Carry out management of producing mushroom.
Through counting, various edible fungus biology efficiency are as shown in table 2:
Table 2
| Group | Mushroom biological efficiency | It picks up the ears biological efficiency | Coprinus comatus biological efficiency | Ganoderma lucidum biological efficiency (dry product meter) |
| Experimental group 1 | 158.6% | 185.7% | 139.2% | 15.1% |
| Experimental group 2 | 146.2% | 166.5% | 130.3% | 13.2% |
| Experimental group 3 | 147.8% | 165.2% | 126.8% | 13.1% |
| Experimental group 4 | 105.9% | 120.1% | 130.7% | 11.6% |
| Experimental group 5 | 146.3% | 162.3% | 100.1% | 13.1% |
Cultivation matrix of the present invention is either rotten to domestomycetes or grass it can be seen from the data of above-mentioned each experimental group
Bacterium all has preferable fruiting effect, and the biological efficiency bacterium of domestomycetes is significantly higher than the cultivation for straw rotting fungus in experimental group 1
The mushroom of the experimental group 4 of matrix is picked up the ears, the biological efficiency of ganoderma lucidum (domestomycetes), at the same in experimental group 1 straw rotting fungus biology
Efficiency bacterium is significantly higher than the biological efficiency of the coprinus comatus (straw rotting fungus) for the experimental group 5 of the cultivation matrix of domestomycetes.
Claims (10)
1. a kind of uninterrupted fruiting matrix of non fast growing tree species hayashishita multi items, which is characterized in that component is as follows, is parts by weight:
180~220 parts of corn core bit, 70~90 parts of broad-leaved wood chip, 70~90 parts of beanstalk bits, 35~45 parts of wheat bran, pulverized limestone 7~
9 parts, 3.5~4.5 parts of land plaster, 0.8~1.2 part of water-retaining agent.
2. uninterrupted fruiting matrix as described in claim 1, which is characterized in that component is as follows, is parts by weight:
200 parts of corn core bit, 80 parts of broad-leaved wood chip, 80 parts of beanstalk bits, 40 parts of wheat bran, 8 parts of pulverized limestone, 4 parts of land plaster, water-retaining agent
1 part.
3. uninterrupted fruiting matrix as described in claim 1, which is characterized in that the partial size of the corncob and soya bean stem meal is little
In 10mm；The partial size of the broad-leaved wood chip is not more than 5mm；
Preferably, water-retaining agent water absorbent rate >=120；
Preferably, the moisture content of the matrix is 57%~63%；
Preferably, the pH value of the matrix is 6.5~7.5；The pH adjusting agent of matrix is to use mass percent concentration for 10%
Acetic acid or mass percent concentration be 5% limewash.
4. the preparation method of uninterrupted fruiting matrix described in claim 1, which is characterized in that steps are as follows:
(1) broad-leaved wood chip is mixed with part pulverized limestone, then in material water quality ratio 1:(0.5~0.7) ratio stir evenly,
Air storage 6~8 days, premix sawdust is made；
(2) corn core bit and beanstalk bits mixs with remaining pulverized limestone, then press and expect water quality ratio 1:(1.0~1.4) ratio
Example stirs evenly, and air storage 4~6 days, premix core material is made；
(3) wheat bran, land plaster are mixed thoroughly jointly, auxiliary material mixture is made；
(4) by water-retaining agent and water 1:(180~220 in mass ratio) ratio mixing, stand 1~2 hour, then with step (1)
The agitated mixing of auxiliary material mixture made from premix sawdust obtained, step (2) premix core material obtained and step (3) to get.
5. preparation method as claimed in claim 4, which is characterized in that in the step (1), pulverized limestone accounts for pulverized limestone gross mass
50%；
Preferably, in the step (1), expect water quality ratio 1:0.6, air storage 7 days；
Preferably, in the step (2), expect water quality ratio 1:1.2, air storage 5 days；
Preferably, in the step (3), the ratio of water-retaining agent and water 1:200 in mass ratio are mixed.
6. the application of the uninterrupted fruiting matrix of non fast growing tree species hayashishita multi items described in claim 1, which is characterized in that step is such as
Under:
(i) the uninterrupted fruiting matrix of non fast growing tree species hayashishita multi items described in claim 1 is packed, is then sterilized, bacterium is made
Stick；
(ii) mushroom strain is accessed into bacteria stick made from step (i), the bacterium germination 125~145 days under the conditions of 5~20 DEG C, alternatively,
The bacterium germination 80~90 days under the conditions of 21~28 DEG C；Then, temperature adjustment to 15~25 DEG C, relative air humidity 70~80%, illumination it is strong
700~900lx, gravity-flow ventilation are spent, is carried out annesl 20~30 days, lentinus edodes strain stick is made；
(iii) into bacteria stick made from step (i), access is picked up the ears strain, the bacterium germination 30~40 days under the conditions of 5~20 DEG C, alternatively,
The bacterium germination 12~18 days under the conditions of 21~28 DEG C；Then, it under the conditions of 0~4 DEG C, carries out mycelia After-mature cultivation 18~22 days, system
Must pick up the ears bacteria stick；
(iv) strain of coprinus comatus is accessed into bacteria stick made from step (i), the bacterium germination 25~35 days under the conditions of 5~20 DEG C, alternatively,
The bacterium germination 12~18 days under the conditions of 21~28 DEG C；Then, it under the conditions of 0~4 DEG C, carries out mycelia After-mature cultivation 18~22 days, system
Obtain coprinus comatus bacteria stick；
(v) lucidum strain is accessed into bacteria stick made from step (i), the bacterium germination 35~45 days under the conditions of 5~20 DEG C, alternatively,
Bacterium germination 22~28 days under the conditions of 21~28 DEG C；Then, it under the conditions of 0~4 DEG C, carries out mycelia After-mature cultivation 18~22 days, is made
Ganoderma lucidum bacteria stick；
(vi) by coprinus comatus made from lentinus edodes strain stick made from step (ii), step (iii) bacteria stick obtained of picking up the ears, step (iv)
Ganoderma lucidum bacteria stick made from bacteria stick and step (v) activates 12~48 hours under normal temperature conditions, then according to lentinus edodes strain stick: Pleurotus sp2
Stick: coprinus comatus bacteria stick: the ratio that ganoderma lucidum bacteria stick quantity ratio is 5: 10: 4: 4 is embedded in canopy density of the area not less than 1000 mu 0.2
~0.7 non fast growing forestland, thickness of earth covering is 2~5cm above bacteria stick, and 0.3~0.5 meter of cultivation cave interval carries out fruiting
Management.
7. application as claimed in claim 6, which is characterized in that in the step (i), the specification of bacteria stick is to prepare the double of bacteria stick
The flat width x length degree of folding diameter specification of layer polybag indicates: lentinus edodes strain stick (20~24) cm × (50~60) cm, bacteria stick of picking up the ears (20~
24) 22cm × (40~50) cm, coprinus comatus bacteria stick (15~20) cm × (25~35) cm, ganoderma lucidum bacteria stick (15~20) cm × (25
~35) cm.
8. application as claimed in claim 6, which is characterized in that in the step (ii), mushroom (Lentinus edodes) choosing
From high temperature type, in wide warm type and/or middle low form kind；It is further preferred that the product name of the high temperature type kind
For " agriculture perfume (or spice) 69 " bacterial strain, it is described in the product name of wide warm type kind be " agriculture perfume (or spice) 66 " bacterial strain, the quotient of the middle low form kind
The name of an article is known as " Shandong flower No.1 " bacterial strain；
Preferably, in the step (iii), pick up the ears selected from high temperature modification kind, low form kind and/or in wide warm type kind；Into
One step is preferred, and the high temperature modification kind is branched oyster mushroom (P.cornucopiae) bacterial strain, and the low form kind is rough skin side
Ear (Pleuotus ostreatus) bacterial strain, it is described in wide warm type kind be pleurotus florida (P.florida) bacterial strain；
Preferably, in the step (iv), coprinus comatus (Coprinus comatus) is selected from coprinus comatus Cc258 and/or long leg chicken
Leg mushroom；
Preferably, in the step (v), ganoderma lucidum is selected from Ganoderma lucidum (Ganoderma lucidum (Leyss.exFr.)
Karst.) bacterial strain, Han Zhi (Ganoderma lucidum) bacterial strain, black sesame (Fructificatio Amaurodermatis
Rudae) bacterial strain and/or green sesame (Coriolus versicolar (L.:Fr.) Quel.) bacterial strain.
9. application as claimed in claim 6, which is characterized in that in the step (vi), 0.5~1, each cultivation cave bacteria stick,
0.5 bacteria stick is after peeling bacteria stick off plastic film, to cut from centre, make into two small stubs, one of them is 0.5 bacteria stick.
10. application as claimed in claim 6, which is characterized in that in the step (vi), the canopy density of non fast growing forestland
0.4~0.7.
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