CN110108632A - A kind of detection method of the plant tissue anti-dehydrating ability based on energy variation - Google Patents
A kind of detection method of the plant tissue anti-dehydrating ability based on energy variation Download PDFInfo
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- CN110108632A CN110108632A CN201910332352.XA CN201910332352A CN110108632A CN 110108632 A CN110108632 A CN 110108632A CN 201910332352 A CN201910332352 A CN 201910332352A CN 110108632 A CN110108632 A CN 110108632A
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N19/00—Investigating materials by mechanical methods
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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Abstract
The detection method for the plant tissue anti-dehydrating ability based on energy variation that the invention discloses a kind of belongs to Drought-resistant Breeding and crop information detection field, comprising the following steps: take plant tissue to be measured to be cleared up with water, and surface moisture is blotted；The maximum bearing capacity of different deformation undertissue is measured, the linear model between deformation quantity and maximum bearing capacity is constructed, obtains elasticity modulus；Fixed deformation is set, the internal force change curve of different dehydration moment plant tissues is measured, obtains probe depression distance；According to above-mentioned linear model and probe depression distance, probe is calculated using definite integral method and pushes accumulation in the process to plant tissue work done；The coefficient of variation of above-mentioned energy between the different dehydration moment is calculated, accordingly the anti-dehydrating ability of quantitative comparison difference plant tissue.The present invention uses physical method, and as a result accuracy is high, simple and quick, can also be detected to the drought-resistant ability of plant according to the anti-dehydrating feature of tissue.
Description
Technical field
The invention belongs to Drought-resistant Breedings and crop information detection technique field, and in particular to a kind of based on energy variation
The detection method of plant tissue anti-dehydrating ability.
Background technique
Arid refers to that long-term precipitation is on the low side, causes to be air-dried, soil water shortage, and the intracorporal moisture of plant is made to wane,
Influence normal growth and development and the underproduction, the underproduction caused by arid be arid and semi-arid lands's grain-production major obstacle it
One.Identification of Drought is exactly to carry out characterization and evaluation by the size of plant drought ability, since plant is in very long evolutionary process
Arid is resisted and adapts in many ways, so form a variety of drought resisting mechanism.
Wherein the anti-dehydrating ability of plant tissue plays an important role in terms of plant is to the adaptation of drought environment.Plant
The anti-dehydrating ability of tissue can be compared by the speed that the flow of water changes, however, leaf water potential is easy quickly by environmental change
Fluctuation；Immediately the index equally frequent quilt such as variation of stomatal conductance, transpiration rate, leaf water content, stem diameter of plant leaf blade
As the indirect detection method for obtaining plant tissue anti-dehydrating ability, measurement perhaps expends the time or lacks accuracy.It is red
Outer thermal image analysis is equally larger by Environmental Factors.Plant electro physiology is widely used in plant growth and development and water regime
Diagnosis, measure the influence vulnerable to clamping device and blade contact area and chucking power, cause measurement error.
Therefore, need to establish measurement a kind of neither affected by environment, and can quickly detecting and have higher accuracy
Method, the anti-dehydrating ability of quantitative comparison difference plant tissue.
Summary of the invention
The technical problem to be solved by the present invention is to provide a kind of inspection of plant tissue anti-dehydrating ability based on energy variation
Survey method can accurately detect the energy variation situation of plant tissue by mechanical index, and then reflect its water regime, easy
Fast, scientific support is provided for plant drought resistance detection technique.
A kind of detection method of the plant tissue anti-dehydrating ability based on energy variation, comprising the following steps:
Step 1, selection is grown fine to measuring plants and consistent tissue is several, and different deformation gradient is arranged, measures each shape
Become the maximum bearing capacity of lower plant tissue；
Step 2 constructs the linear model between deformation quantity and maximum bearing capacity, obtains the elasticity modulus of plant tissue；
Dehydration processing is dried to selected plant tissue to be measured in step 3；
Fixed deformation is arranged in step 4, measures plant tissue in the internal force change curve at different dehydration moment, is popped one's head in
Depression distance；
Step 5 is calculated during probe pushes and is accumulated to plant tissue work done, as energy；
Step 6 calculates the coefficient of variation of energy between the different dehydration moment；
Step 7, the anti-dehydrating ability of quantitative comparison difference plant tissue.
Further, the setting for fixing deformation in the step 1 in different deformation gradient and step 4 must be with plant group
It knits not premised on by irreversible damage.
Further, the linear model in the step 2 is Hooke's law formula F=kx, and wherein F is pressure, and x is deformation,
K is elasticity modulus.
Further, the probe depression distance in the step 4 are as follows: measurement tissue internal force change curve pressure probe with
Until plant tissue contact start to the position for pressing to set fixation deformation, the distance that is run therebetween.
Further, in the step 5, the calculation formula of function are as follows: W=FS, wherein W is function, and F is pressure, and S is under probe
Press distance.
Further, in the step 5, during probe pushes, pressure increases with the increase of probe depression distance,
Its variation meets Hooke's law, and definite integral method can be used and calculate probe accumulation to plant tissue work done, i.e.,Wherein b is institute after probe and plant tissue contact start to the position for pressing to set fixation deformation
The maximum distance of operation.
Further, in the step 6, the calculation formula of the coefficient of variation of energy are as follows:Wherein CV is to become
Different coefficient, SD are standard deviation, and MN is the average value of the function at different dehydration moment in step 5.
Further, in the step 7, the anti-dehydrating ability of different plant tissues passes through variation calculated in step 6
Coefficient carries out quantitative comparison.
Advantages of the present invention is as follows:
1) this method be based on organization mechanics index computation organization anti-dehydrating ability, as a result not by measurement surrounding equipment environment because
Son limitation, as a result accurately, high reliablity.
2) measurement of this method based on tissue elasticity modulus, pressure work done during computation organization is pressed, according to
Conservation of energy principle can be preferably by the change of tissue energy caused by moisture to analyze the moisture situation of change of plant tissue
Change and connected with the anti-dehydrating ability of plant, preferably adaptability of the reflection plant to drought environment.
3) this method calculates the moisture changing condition for obtaining plant tissue based on mechanical characteristic, by physics and plant physiology
It learns and organically combines, the test process of plant physiology index is more simple and direct.
Detailed description of the invention
Fig. 1 is the graph of relation between deformation quantity and maximum bearing capacity using Hooke's law formula fitting；
Fig. 2 is the internal force change curve of 0 dehydration moment ivy stem.
Specific embodiment
Concrete scheme of the invention is described further below in conjunction with attached drawing.
Basic principle of the invention are as follows:
Plant passes through long-term evolution, all from formalness, anatomic construction and from internal physiological biochemical characteristic etc.
Form the enduring dehydrate ability under the delaying dehydrate and the low flow of water under the high flow of water.Wall mechanical intensity is higher, maintenance group
The ability for knitting turgescence is stronger, and the energy variation situation of plant tissue caused by moisture is measured by mechanical index, is able to reflect
The resistance to fracture ability of cell wall characterizes Genes For Plant Tolerance water separation capability.
For plant tissue by cell composition, the cell wall for constituting cell is an elastic membrane.Therefore, plant tissue cell is whole
On can be considered as elastic material, in loading process, before plant tissue wrecks, meet between external pressure and tissue deformation
Hooke's law formula.
Hooke's law formula are as follows:
F=kx (1)
Wherein F is pressure, and x is deformation, and k is elasticity modulus.
The calculation formula of function are as follows:
W=FS (2)
Wherein W is function, and F is pressure, and S is the pressure probe and plant tissue contact start for measuring tissue internal force change curve
Until the position for pressing to set fixation deformation, the distance that is run therebetween.
During probe pushes, pressure increases in real time with the increase of depression distance, and variation meets Hooke's law, can
It is calculated by equation F=kS.
It is arranged in the case of fixed deformation, presses to plant tissue, what probe did plant tissue accumulation during pushing
Function can be used definite integral method and be calculated, it may be assumed that
Wherein b is probe and plant tissue contact start to after pressing to the position of set fixation deformation, is run
Maximum distance.
In plant tissue dehydration process, the variation of its internal physiological and energy can be caused.The mechanical strength of plant tissue is got over
The resistance to fracture ability of height, cell wall is stronger, and anti-dehydrating ability is stronger.To tissue course of exerting pressure in, under same deformation, pressure
It is more to tissue work done.Therefore, under same deformation, different dehydration moment pressure are to plant tissue work done, that is, energy
Variation between consumption is more violent, reflects that the variation of its moisture is more significant, tissue anti-dehydrating ability is weaker.Amplitude of variation can pass through
The coefficient of variation is calculated, and formula is as follows:
Wherein CV is the coefficient of variation, and SD is standard deviation, and MN is the forces on tissue work done at different dehydration moment
Average value.
It by the comparison of CV between different plant tissues, that is, can determine that its anti-dehydrating ability, reflect drought resistance.
Specific implementation process of the invention is as follows:
Step 1 takes the flesh tissue to measuring plants of normal growth, and encases plant limb base portion with wet cloth, slows down water
Dispersion hair；It is rapidly returned to laboratory, after clearing up the tissue surface dust, more with growing way on the scissors interception flesh tissue
Consistent segment is several, is put into moisturizing in wet cloth.Different deformation gradient is set, measures each deformation undertissue most using Texture instrument
Big bearing capacity；Wherein, the setting of each deformation quantity must be to organize not premised on by irreversible damage.
Step 2 constructs the linear model between deformation quantity and maximum bearing capacity, obtains the elasticity modulus of tissue；Linear mould
Type is Hooke's law, i.e. formula (1).
Dehydration processing is dried to selected plant tissue to be measured in step 3.
Fixed deformation is arranged in step 4, and the internal force using Texture instrument measurement plant tissue at the different dehydration moment changes bent
Line obtains probe depression distance；Wherein, the setting of fixed deformation equally must be to organize not premised on by irreversible damage；Probe
Depression distance be measure tissue internal force change curve probe and plant tissue contact start arrive press to set by fixation shape
Until the position of change, the distance that is run therebetween.
Step 5 is calculated during probe pushes and is accumulated to plant tissue work done, as energy；Function is calculated as public affairs
Formula (2)；During probe pushes, pressure increases with the increase of probe depression distance, and variation meets Hooke's law, can adopt
Probe accumulation is calculated to plant tissue work done, i.e. formula (3) with definite integral method.
Step 6 calculates the coefficient of variation of energy between the different dehydration moment；The calculation formula of the coefficient of variation of energy is public affairs
Formula (4).
Step 7, according to the anti-dehydrating energy between coefficient of variation quantitative comparison difference plant tissue calculated in step 6
Power.
Embodiment:
The ivy of normal growth and the fresh braches of epipremnum aureum are taken, and encases plant limb base portion with wet cloth, slows down moisture
It distributes；It is rapidly returned to laboratory, after clearing up the axis surface dirt, more with growing way on the scissors interception fresh braches
Totally 10 sections of consistent stem 10mm, it is put into moisturizing in wet cloth.It is arranged different deformation gradient (0,5%, 10%, 15%, 20%), measurement
The maximum bearing capacity (being shown in Table 1) of stem under each deformation.
The maximum bearing capacity (N) of ivy and epipremnum aureum stem under each deformation of table 1
The relationship between deformation quantity and maximum bearing capacity is fitted using Hooke's law formula, matched curve such as Fig. 1.
The fit equation of ivy and epipremnum aureum is respectively F=92.23x, R2=0.90, P < 0.0001 and F=28.54x, R2=
0.91, P < 0.0001.Therefore the elasticity modulus k value of ivy and epipremnum aureum stem is respectively 92.23 and 28.54.
Dehydration processing is dried to selected axis to be measured；Fixed shape, which is arranged, becomes 22%, measures two kinds of plants respectively
Object stem obtains probe depression distance in the internal force change curve at different dehydration moment.Become with the internal force of 0 dehydration moment ivy stem
Figure is done for change curve, sees Fig. 2.The depression distance popped one's head under two kinds of fixed deformation of plant difference dehydration moment setting is obtained, is seen
Table 2.
The depression distance (mm) popped one's head under fixed deformation is arranged in 2 two kinds of plant difference dehydration moment of table
During probe pushes, pressure increases with the increase of probe depression distance, and variation meets Hooke's law, can
Using definite integral method, calculates separately during probe pushes under the different dehydration moment and accumulate to two kinds of axis work done, see
Table 3.
Accumulation is to two kinds of axis work done (10 during probe pushes under the different dehydration moment of table 3-3J)
Pressure can to axis work done between calculating the different dehydration moment by the calculation formula of the coefficient of variation of energy
The coefficient of variation for measuring consumption, is shown in Table 4.
Pressure is to axis work done, that is, energy consumption coefficient of variation between the different dehydration moment of table 4
As shown in Table 4, pressure is low to ivy stem work done, that is, energy consumption coefficient of variation between the different dehydration moment
Moisture in epipremnum aureum, ivy stem is more stable, shows that it, can be in drought environment with stronger anti-dehydrating ability
It is lower to maintain preferable moisture retention properties, embody preferable drought resistance.This is consistent with actual conditions.
Described above is only that presently preferred embodiments of the present invention should be said the present invention is not limited to enumerate above-described embodiment
Bright, under the introduction of this specification, all equivalent substitutes for being made obvious become anyone skilled in the art
Shape form, all falls within the essential scope of this specification, ought to be protected by the present invention.
Claims (8)
1. a kind of detection method of the plant tissue anti-dehydrating ability based on energy variation, which comprises the following steps:
Step 1, selection is grown fine to measuring plants and consistent tissue is several, and different deformation gradient is arranged, measures under each deformation
The maximum bearing capacity of plant tissue；
Step 2 constructs the linear model between deformation quantity and maximum bearing capacity, obtains the elasticity modulus of plant tissue；
Dehydration processing is dried to selected plant tissue to be measured in step 3；
Fixed deformation is arranged in step 4, measures internal force change curve of the plant tissue at the different dehydration moment, obtains probe and pushes
Distance；
Step 5 is calculated during probe pushes and is accumulated to plant tissue work done, as energy；
Step 6 calculates the coefficient of variation of energy between the different dehydration moment；
Step 7, the anti-dehydrating ability of quantitative comparison difference plant tissue.
2. the detection method of the plant tissue anti-dehydrating ability according to claim 1 based on energy variation, feature exist
In the setting for fixing deformation in the step 1 in different deformation gradient and step 4 must be with plant tissue not by irreversible
Premised on damage.
3. the detection method of the plant tissue anti-dehydrating ability according to claim 1 based on energy variation, feature exist
In the linear model in the step 2 is Hooke's law formula F=kx, and wherein F is pressure, and x is deformation, and k is elasticity modulus.
4. the detection method of the plant tissue anti-dehydrating ability according to claim 1 based on energy variation, feature exist
In probe depression distance in the step 4 are as follows: the pressure probe of measurement tissue internal force change curve is contacted with plant tissue
Start the distance run therebetween until the position for pressing to set fixation deformation.
5. the detection method of the plant tissue anti-dehydrating ability according to claim 1 based on energy variation, feature exist
In, in the step 5, the calculation formula of function are as follows: W=FS, wherein W is function, and F is pressure, and S is probe depression distance.
6. the detection method of the plant tissue anti-dehydrating ability according to claim 1 or 3 based on energy variation, feature
It is, in the step 5, during probe pushes, pressure increases with the increase of probe depression distance, and variation meets
Hooke's law can be used definite integral method and calculate probe accumulation to plant tissue work done, i.e.,Wherein b is
The maximum distance run after probe and plant tissue contact start to the position for pressing to set fixation deformation.
7. the detection method of the plant tissue anti-dehydrating ability according to claim 1 based on energy variation, feature exist
In, in the step 6, the calculation formula of the coefficient of variation of energy are as follows:Wherein CV is the coefficient of variation, and SD is mark
Quasi- deviation, MN are the average value of the function at different dehydration moment in step 5.
8. the detection method of the plant tissue anti-dehydrating ability according to claim 1 based on energy variation, feature exist
In in the step 7, the anti-dehydrating ability of different plant tissues is quantified by the coefficient of variation calculated in step 6
Compare.
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