CN110100818B - Lygus pratensis attractant and preparation method and application thereof - Google Patents
Lygus pratensis attractant and preparation method and application thereof Download PDFInfo
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- CN110100818B CN110100818B CN201910473477.4A CN201910473477A CN110100818B CN 110100818 B CN110100818 B CN 110100818B CN 201910473477 A CN201910473477 A CN 201910473477A CN 110100818 B CN110100818 B CN 110100818B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
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Abstract
The embodiment of the invention discloses a lygus pratensis attractant and a preparation method and application thereof, wherein the raw materials for preparing the lygus pratensis attractant comprise sunflower aldehyde and 3-hexene-1-alcohol, and the volume ratio of the sunflower aldehyde to the 3-hexene-1-alcohol is (0.5-1.5): (4-6). Especially, the effect is better when the volume ratio of the sunflower aldehyde to the 3-hexene-1-alcohol is 1:5, and the sunflower aldehyde and the 3-hexene-1-alcohol are respectively diluted into 1:1000 times of liquid before use. The lygus pratensis attractant provided by the embodiment of the invention uses sunflower aldehyde and 3-hexene-1-alcohol as main components, has a good trapping effect, and can achieve the purpose of controlling pests by trapping and killing adult pests in a field.
Description
Technical Field
The embodiment of the invention relates to the technical field of agriculture, and particularly relates to a lygus pratensis attractant and a preparation method and application thereof.
Background
Lygus is the 1 family of hemiptera of the class insecta. The front wing is provided with a wedge, and no single-eye ground bug is generated. Commonly known as lygus lucorum. The hemiptera is the largest family, nearly ten thousand species are known in the world, and all large regions are distributed. Most of lygus insects live on plants, are active in action and fly well. Most groups are mainly phytophagous and have a wide host range, including angiosperms, conifers and ferns. Besides sucking the leaves of plants, the plants like the flowers, buds, fruits and other reproductive organs are favored, and many varieties have obvious habits of chasing flowering plants. In addition to eating vegetable food, many species often eat other small soft insects, and this habit is particularly pronounced during the reproductive period. Thus, even some harmful species may benefit humans by predating other pests. Some groups are mainly predatory and become natural enemies of pests such as aphids.
The lygus pratensis belongs to lygus pratensis, the life habit is 3-4 generations of north 1 year, and adults live through the winter under weeds, dry branches and fallen leaves and soil stones. In next spring, the host takes out the hibernation and moves, the juice is absorbed by stinging on tender leaves, tender stems and flower buds, after a period of time, the host begins to copulate and lay eggs, the eggs are produced in the tender stems, leaf stalks, leaf veins or buds in more than 10 days. Nymphs aged 5 years altogether and emerged into adults over 30 days. Adults and nymphs like to move in the daytime, eat most in the morning and at night, move quickly and are good at hiding. The emergence period is irregular, the cotton field is often transplanted in 6 months, and the woody plant or autumn vegetables are transplanted in autumn. The natural enemies mainly comprise egg parasitic wasps, predatory spiders, adelphocoris suturalis, lygus lucorum and the like.
At present, chemical pesticides are generally adopted for controlling lygus pratensis in the control process of lygus pratensis, but the large and unreasonable application of chemical agents causes serious pollution to the ecological environment of farmlands.
Disclosure of Invention
Therefore, the embodiment of the invention provides the lygus pratensis attractant and the preparation method and the application thereof, so as to solve the problem that the farmland ecological environment is seriously polluted by adopting chemical pesticides in the prior art.
In order to achieve the above object, the embodiments of the present invention provide the following technical solutions:
according to a first aspect of the embodiments of the present invention, there is provided a lygus pratensis attractant, wherein raw materials for preparing the lygus pratensis attractant comprise sunflower aldehyde and 3-hexen-1-ol, and the volume ratio of the sunflower aldehyde to the 3-hexen-1-ol is (0.5-1.5): (4-6).
Furthermore, the volume ratio of the sunflower aldehyde to the 3-hexen-1-ol is 1: 5.
Further, the volume fractions of the components in the raw materials for preparing the lygus pratensis attractant are respectively as follows: 0.5 part of sunflower aldehyde and 4 parts of 3-hexene-1-alcohol.
Further, the volume fractions of the components in the raw materials for preparing the lygus pratensis attractant are respectively as follows: 0.5 part of sunflower aldehyde and 6 parts of 3-hexene-1-alcohol.
Further, the volume fractions of the components in the raw materials for preparing the lygus pratensis attractant are respectively as follows: 1.5 parts of sunflower aldehyde and 4 parts of 3-hexene-1-alcohol.
Further, the volume fractions of the components in the raw materials for preparing the lygus pratensis attractant are respectively as follows: 1.5 parts of sunflower aldehyde and 6 parts of 3-hexene-1-alcohol.
According to a second aspect of the embodiments of the present invention, there is provided a preparation method of lygus pratensis attractant, comprising the following steps:
step one, respectively diluting sunflower aldehyde and 3-hexene-1-alcohol into 1:1000 times of solution for later use;
step two, mixing the sunflower aldehyde and the 3-hexene-1-alcohol according to the weight ratio of (0.5-1.5): and (4-6) mixing the components uniformly according to the volume ratio to prepare the lygus pratensis attractant.
According to a third aspect of embodiments of the present invention, there is provided a use of lygus pratensis attractant for trapping lygus pratensis.
According to a fourth aspect of embodiments of the present invention, there is provided an attractant comprising the lygus pratensis attractant described above.
The embodiment of the invention has the following advantages: the lygus pratensis attractant uses sunflower aldehyde and 3-hexen-1-ol as main components, has a good trapping effect, can reduce the application times and the using amount of pesticides by using the lygus pratensis attractant, reduces ecological pollution, and achieves the purpose of preventing and controlling pests by trapping and killing adults in fields.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be apparent that the drawings in the following description are merely exemplary, and that other embodiments can be derived from the drawings provided by those of ordinary skill in the art without inventive effort.
FIG. 1 shows a graph 10 of the first experiment of the present invention-1(V/V) field trapping effect of plant volatile substances with concentration on the lygus pratensis male and female adults;
FIG. 2 shows a graph 10 of the first experiment of the present invention-2(V/V) field trapping effect of plant volatile substances with concentration on the lygus pratensis male and female adults;
FIG. 3 shows a graph 10 of the first experiment of the present invention-3(V/V) field trapping effect of plant volatile substances with concentration on the lygus pratensis male and female adults;
FIG. 4 shows a graph 10 of the first experiment of the present invention-4(V/V) field trapping effect of plant volatile substances on the lygus pratensis male and female adults.
Detailed Description
The present invention is described in terms of particular embodiments, other advantages and features of the invention will become apparent to those skilled in the art from the following disclosure, and it is to be understood that the described embodiments are merely exemplary of the invention and that it is not intended to limit the invention to the particular embodiments disclosed. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The embodiment provides a lygus pratensis attractant, raw materials for preparing the lygus pratensis attractant comprise sunflower aldehyde and 3-hexen-1-ol, and the volume ratio of the sunflower aldehyde to the 3-hexen-1-ol is (0.5-1.5): (4-6).
Example 2
The embodiment provides a lygus pratensis attractant, wherein the volume ratio of the sunflower aldehyde to the 3-hexen-1-ol is 1: 5.
Example 3
The embodiment provides a lygus pratensis attractant, and the volume fractions of the components in the raw materials for preparing the lygus pratensis attractant are respectively as follows: 0.5 part of sunflower aldehyde and 4 parts of 3-hexene-1-alcohol.
Example 4
The embodiment provides a lygus pratensis attractant, and the volume fractions of the components in the raw materials for preparing the lygus pratensis attractant are respectively as follows: 0.5 part of sunflower aldehyde and 6 parts of 3-hexene-1-alcohol.
Example 5
The embodiment provides a lygus pratensis attractant, and the volume fractions of the components in the raw materials for preparing the lygus pratensis attractant are respectively as follows: 1.5 parts of sunflower aldehyde and 4 parts of 3-hexene-1-alcohol.
Example 6
The embodiment provides a lygus pratensis attractant, and the volume fractions of the components in the raw materials for preparing the lygus pratensis attractant are respectively as follows: 1.5 parts of sunflower aldehyde and 6 parts of 3-hexene-1-alcohol.
Example 7
The embodiment provides a preparation method of lygus pratensis attractant, which comprises the following steps:
step one, respectively diluting sunflower aldehyde and 3-hexene-1-alcohol into 1:1000 times of solution for later use;
step two, mixing the sunflower aldehyde and the 3-hexene-1-alcohol according to the weight ratio of (0.5-1.5): and (4-6) mixing the components uniformly according to the volume ratio to prepare the lygus pratensis attractant.
Example 8
The embodiment provides application of the lygus pratensis attractant to trapping lygus pratensis.
Example 9
The present example provides an attractant comprising the lygus pratensis attractant described above.
The lygus pratensis attractant provided by the embodiment utilizes the sunflower aldehyde and the 3-hexene-1-alcohol as main components, so that the trapping effect of the attractant can be greatly improved, convenience is brought to production of people, and the crop yield is improved.
Experiment one
31 active substances are identified from lygus pratensis hosts, 16 volatile matters are selected, the 16 volatile matters and the compound thereof are subjected to field trapping effect on the basis of research on olfactory action reaction and antenna potential reaction of lygus pratensis adults, and a theoretical basis is provided for developing comprehensive treatment measures of lygus pratensis.
1 test Material
1.1 Experimental materials
Two sides of each color plate of the white pest sticking plate are coated with a layer of 0.4mm pest sticking glue, and the pest sticking plate is a rectangle of 24cm multiplied by 40 cm. Small test tube, bamboo stick, iron wire.
1.2 test reagents
Test reagents: according to the identification of 31 kinds of information compounds in 7 kinds of plant volatiles of lygus pratensis by Sunpeng et al, 16 active substances are selected as test reagents in the experiment, and the test reagents are shown in Table 1.
Table 1 Standard Compound Table2-1the Standard chemical sampling experiment
1.3 field trapping effect of sixteen plant volatile substances on lygus pratensis
The insect sticking plate is white, has the length and the width respectively, is provided with insect sticking glue on the front side and the back side, and is tested in the peripheral garden nursery bases of Tarim university. During the test, no farm work operation such as chemical pesticide spraying, topping and the like and field investigation outside the test are carried out, so that the influence of human interference on the test is avoided. Before the insect sticking board is used in the field, a long wood pole is inserted into the soil and fixed and kept vertical, then the insect sticking board is fixed on the wood pole by a thin iron wire, the bottom of the insect sticking board is slightly higher than the top of weeds, the distance between every two adjacent poles is 15m, and the insect sticking board has an adhesive surface facing south. The plate was centrally suspended with 1ml of a control of the active substance diluted with lanolin paste, which was placed in a 1.5ml sample bottle with the mouth slightly inclined downwards. And 3d, carrying out sign and statistics on the lygus pratensis trapped on the sticky trap. Each active substance analogue was repeated 6 times. The Duncan new double-pole difference method of the DPS6.55 statistical software detects the difference significance.
1.4 field trapping effect of sixteen plant volatile substances on lygus pratensis in concentration ratio
N-nonadecane, n-decanal, 3-ethylene-1-ol, docosane and beta-pinene in different concentrations and proportions are subjected to field trapping tests.
2 results and analysis
2.1 Devicid grass male and female imago pairs 10-1(V/V) field trapping Effect of plant volatile substances
The concentration of the volatile matter in the tested 16 lygus pratensis host plants was 10-1(V/V), the effect of trapping male and female adults with n-decanal was all significantly different from that of the control paraffin (P)<0.05). The trapping effect of the female insects is greater than that of the male insects in the experiment.
The results are shown in FIG. 1, in which 1: tetratetratetratetratetratetratetratetratetratetracontan; octacosane; 3: heptacosane Heptacosan; 4: nonadecane nonacan; 5: stearamide Octadec anamid; 6: spirolactone Spironolactone; 7: heptadecane heptadecen; 8: decanol, deca, lbeta; 9: ionone 3-Buten-2-one, 4- (2, 6, 6-trimethy1-1-cyclohexen-1-yl) -; 10: (Z) -3-hexenyl butyrate-3-enoic acid, 3-hexenylester, (Z) -; 11; 3-Hexen-1-ol; 12: (E) -4-Hexen-1-ol, (E); 13; dodecane; 14: beta-Pinene; 15: z-3-methylbutyrate-3-hexenyl ester cis-3-Hexenylisoverate; 16: pentadecane pentadecene, wherein the data in the figure are mean values +/-standard errors; different letters showed significant differences between different plant volatiles (Kruskal-Wallis test, P < 0.05). FIGS. 2, 3 and 4 are the same.
2.2 Devicid male and female adult pairs 10-2(V/V) field trapping Effect of plant volatile substances
The results are shown in FIG. 2, note: data are mean ± sem; different letters showed significant differences between different plant volatiles (Kruskal-Wallis test, P < 0.05).
The concentration of the volatile matter in the tested 16 lygus pratensis host plants was 10-2In the (V/V), the effect of trapping male and female adults by beta-pinene is all different remarkably compared with the control paraffin (P)<0.05). The trapping effect of the female insects is greater than that of the male insects in the experiment.
2.3 Devicid grass male and female adult pairs 10-3(VV) field trapping effect of plant volatile substances at concentration
The concentration of the volatile matter in the tested 16 lygus pratensis host plants was 10-3(V/V) shows that the trapping effects of heptadecane, n-decanal, beta-pinene, Z-3-methylbutyrate-3-hexenyl ester and pentadecane on male and female adults are all different remarkably from those of the control paraffin (P)<0.05), the number of the male larvae per plate reaches more than 4. The trapping effect of the female insects is greater than that of the male insects in the experiment.
The results are shown in FIG. 3, noting: data are mean ± sem; different letters showed significant differences between different plant volatiles (Kruskal-Wallis test, P < 0.05).
2.4 Devicid grass male and female adult pairs 10-4(V/V) field trapping Effect of plant volatile substances
The concentration of the volatile matter in the tested 16 lygus pratensis host plants was 10-4(V/V), the effect of the stearamide, the (Z) -butyric acid-3-hexenyl ester, the dodecane and the beta-pinene on trapping the male and female adults of the lygus pratensis is obvious in average difference compared with the control paraffin (P)<0.05). The trapping effect of the female insects is greater than that of the male insects in the experiment.
The results are shown in FIG. 4, noting: data are mean ± sem; different letters showed significant differences between different plant volatiles (Kruskal-Wallis test, P < 0.05).
2.5 analysis of plant volatile substances with good trapping effect on adult male and female lygus pratensis
In the tested 16 lygus pratensis host plant volatile matters with different concentrations, 8 volatile matters with better trapping effect and different concentrations are screened out, wherein the concentration is mainly 10-3And 10-4(V/V) was more potent in trapping than other concentrations. The trapping effect is best, the trapping number of the heptadecane, the sunflower aldehyde and the female insect reaches 4 per plate, and the difference with other volatile matters is obvious (p)<0.05)。
Table 1: plant volatile substance with optimal trapping effect on lygus pratensis male and female adults
Data are mean ± sem; different letters showed significant differences between different plant volatiles (Kruskal-Wallis test, P < 0.05). The same goes for
2.6 field trapping effect of adelphocoris suturalis male and female adults on volatile substances of different host plants in different concentration ratios
The trapping effect of the four volatile substances on the adult lygus pratensis in different concentrations and proportions by using a field trapping method is preliminarily determined (see table 2). From the measurement results, the field trapping effect of the female lygus pratensis adults to volatile substances is better than that of the male lygus pratensis adults in most proportion, and the field trapping effect in most proportion in the experiment has obvious reaction difference (P)<0.05), wherein malvalic aldehyde 10-33-hexen-1-ol 10-3(1:5) nonadecane 10-3Beta-pinene 10-4Dodecane 10-3(1:1:5), nonadecane 10-3Beta-pinene 10-4Dodecane 10-3(1:5:1), the trapping effect on the adelphocoris pratensis adult female and male is good, the average number of the female insects in each plate is more than 5, and the differences from other compounds are obvious (P)<0.05)。
Table 2.5 field trapping effect of different proportions of volatile substances on adelphocoris praecox adults
Note: data are mean ± sem; different letters showed significant differences between different plant volatiles (Kruskal-Wallis test, P < 0.05).
3 small knot
The experiment carries out field trapping effect test on 16 volatile substances of the lygus pratensis host plant, and selects plant volatile substances with different concentrations, wherein the plant volatile substances comprise 10 of heptadecane, sunflower aldehyde and beta-pinene-3The trapping effect is good. Screening plant volatile substances with different concentration ratios in trapping effectSunflower aldehyde 10-33-hexen-1-ol 10-3(1:5) nonadecane 10-3Beta-pinene 10-4Dodecane 10-3(1:1:5), nonadecane 10-3Beta-pinene 10-4Dodecane 10-3(1:5:1) has good trapping effect on male and female adults, the trapping quantity of the male worms in each board reaches more than 5, and the three proportions are obviously different from other proportions (P)<0.05)。
Although the invention has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (7)
1. The lygus pratensis attractant is characterized in that raw materials for preparing the lygus pratensis attractant comprise sunflower aldehyde and 3-hexene-1-alcohol, wherein the volume ratio of the sunflower aldehyde to the 3-hexene-1-alcohol is (0.5-1.5): (4-6);
the preparation method of the lygus pratensis attractant comprises the following steps:
step one, respectively diluting sunflower aldehyde and 3-hexene-1-alcohol into 1:1000 times of solution for later use;
step two, mixing the sunflower aldehyde and the 3-hexene-1-alcohol according to the weight ratio of (0.5-1.5): and (4-6) mixing the components uniformly according to the volume ratio to prepare the lygus pratensis attractant.
2. The lygus pratensis attractant according to claim 1, wherein the volume ratio of the decanol to the 3-hexen-1-ol is 1: 5.
3. The lygus pratensis attractant according to claim 1, wherein the volume fractions of the components in the raw materials for preparing the lygus pratensis attractant are respectively: 0.5 part of sunflower aldehyde and 4 parts of 3-hexene-1-alcohol.
4. The lygus pratensis attractant according to claim 1, wherein the volume fractions of the components in the raw materials for preparing the lygus pratensis attractant are respectively: 0.5 part of sunflower aldehyde and 6 parts of 3-hexene-1-alcohol.
5. The lygus pratensis attractant according to claim 1, wherein the volume fractions of the components in the raw materials for preparing the lygus pratensis attractant are respectively: 1.5 parts of sunflower aldehyde and 4 parts of 3-hexene-1-alcohol.
6. The lygus pratensis attractant according to claim 1, wherein the volume fractions of the components in the raw materials for preparing the lygus pratensis attractant are respectively: 1.5 parts of sunflower aldehyde and 6 parts of 3-hexene-1-alcohol.
7. Use of the lygus pratensis attractant according to any one of claims 1 to 6 for trapping lygus pratensis.
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