CN115024319A - Application of lygus pratensis host plant volatile matter - Google Patents
Application of lygus pratensis host plant volatile matter Download PDFInfo
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- CN115024319A CN115024319A CN202210655048.0A CN202210655048A CN115024319A CN 115024319 A CN115024319 A CN 115024319A CN 202210655048 A CN202210655048 A CN 202210655048A CN 115024319 A CN115024319 A CN 115024319A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P19/00—Pest attractants
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- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
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- A01N37/30—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof containing the groups —CO—N< and, both being directly attached by their carbon atoms to the same carbon skeleton, e.g. H2N—NH—CO—C6H4—COOCH3; Thio-analogues thereof
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Abstract
The invention discloses application of a lygus pratensis host plant volatile matter. The application of the lygus pratensis host plant volatile matter in controlling lygus pratensis comprises any one or more of phenylacetaldehyde, ocimene, methyl salicylate, butyl isothiocyanate, trans-2-hexenol and linalool. According to the invention, through researching volatile chemical substances of five lygus lucorum host plants, namely rape, cotton, black nightshade, chenopodium glaucum and purslane, a compound with an obvious trapping effect on lygus lucorum is screened out, and the compound can be used as an attractant for lygus lucorum and provides a new way for effectively controlling lygus lucorum.
Description
Technical Field
The invention relates to the technical field of plant disease and insect pest control, in particular to application of a lygus pratensis host plant volatile matter.
Background
Lygus pratensis is an important agricultural pest in Xinjiang, in the 50-70 th ages of 20 th century, Lygus pratensis is a serious pest to cotton, the maximum quantity of hundreds of plants can reach 190, the pest rate of cotton plants is almost 100 percent, the pest rate is a main reason for cotton bud and boll shedding, a cotton field is not controlled timely, and the yield is reduced by more than 50 percent; in the 80-90 s, along with outbreak and corresponding chemical control of cotton aphids and cotton bollworms in Xinjiang, lygus pratensis are treated at the same time, the population quantity is reduced, the harm is reduced, and the lygus pratensis become secondary pests. In the 21 st century, with the adjustment of planting structure of crops in Xinjiang, the change of farming system, especially the large-area planting of transgenic cotton, the urbanization construction, the change of climatic conditions and other factors, lygus pratensis again develops disasters. The damage rate of the cotton field bolls reaches 56%, the cotton field bolls are transferred to orchards such as red dates, walnuts, bergamot pears and the like, the fruit trees are damaged and aggravated, and damaged fruits grow up to form hericium erinaceus and pimple pears, which seriously threatens the sustainable development of Xinjiang cotton and forest fruit industries.
Plant volatiles play an important role in the host selection process of phytophagous insects. The phytophagous insects are eventually able to find and reach the host plant, often where volatile substances released by the plant play an important role, helping the phytophagous insects to orient the host plant. For example, the host orientation of potato beetles to potatoes is dependent on the odor produced by the leaves of the potato plant; the diamondback moth Plutella xylostella has directional behavior on mustard oil, namely isothiocyanate, emitted by crucifers; trans-2 hexenal, nonanal, benzaldehyde, linalool in cabbage have strong attraction to Spodoptera litura. Research on behavior regulation of plant volatile substances on lygus insects has also increased significantly in recent years. Studies found that the female lygus lucorum appeared sensitive to host plant volatiles, while the male reacted slowly. Researches such as Sunpeng and the like show that the adult lygus pratensis has the largest EAG reaction value caused by acetic acid cis-3-hexenyl ester and nonanal, and the difference between female and male is obvious; in the olfactory behavior determination, the behavioral reaction difference of lygus pratensis on most concentrations of nonanal, farnesene, alpha-pinene, 1-caryophyllene, cis-3-hexenyl acetate and 2-methyl-1-ol is obvious, and the behavioral reaction difference of the andrid only on partial concentrations of the alpha-pinene and the nonanal is obvious, so that a scientific basis is provided for the research and development of lygus pratensis attractant or repellent strategy.
Therefore, the plant volatile matter with trapping effect on the lygus pratensis is screened out, can be used as the lygus pratensis attractant, and has important significance on the control of the lygus pratensis.
Disclosure of Invention
The invention aims to provide application of a lygus pratensis host plant volatile matter to solve the problems in the prior art.
In order to achieve the purpose, the invention provides application of lygus pratensis host plant volatile matters in controlling lygus pratensis, wherein the lygus pratensis host plant volatile matters comprise any one or more of phenylacetaldehyde, ocimene, methyl salicylate, butyl isothiocyanate, trans-2-hexenol and linalool.
Further, application of phenylacetaldehyde and/or butyl isothiocyanate in prevention and treatment of lygus pratensis female.
Further, application of phenylacetaldehyde in controlling lygus pratensis female insects.
Further, the concentration of phenylacetaldehyde is 100 +/-10 mu L/mL.
Further, the concentration of the butyl isothiocyanate is 100 +/-10 mu L/mL.
A second aspect of the invention provides the use of a lygus pratensis host plant volatile as described above in the preparation of a lygus pratensis attractant.
The invention has the following advantages:
according to the invention, through researching volatile chemical substances of five lygus lucorum host plants, namely rape, cotton, black nightshade, chenopodium glaucum and purslane, a compound with an obvious trapping effect on lygus lucorum is screened out, and the compound can be used as an attractant for lygus lucorum and provides a new way for effectively controlling lygus lucorum.
Drawings
Fig. 1 is the volatile GC-MS detection profiles of 5 host plants of lygus pratensis, wherein, rape a; b, chenopodium glaucum; c purslane; d, black nightshade; and E, cotton.
FIG. 2 is the antennal potential response of plant bug females and males to 6 host plant volatile substances, wherein 1 Butyl isothiocyanate sec-Butyl, 2 Methyl salicylate, 3 Phenylacetaldehyde phenyl acetate, 4 Ocimene Ocine, 5 trans-2-hexenol trans-2-Hexen-1-ol, 6 Linalool linaol.
Detailed Description
The present invention is described in terms of specific embodiments, and other advantages and benefits of the present invention will become apparent to those skilled in the art from the following disclosure. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the following examples:
test material
A test insect source: the lygus pratensis is collected from ten roadside weeds of the first soldier of the production and construction of Xinjiang, and the collected adults are put into an insect cage and are placed into an artificial climate box for feeding. The breeding conditions are as follows: temperature 27 + -1 deg.C, humidity 60 + -5%, and illumination 16:8(L: D).
Test host plants: rape, cotton, black nightshade, chenopodium glaucum and purslane.
Instrumentation and equipment
Gas chromatography-triple quadrupole mass spectrometer TSQ 9000(Thermo scientific, USA), digital display constant temperature water bath (OLABO, Shandong), manual SPME sample injector, 50/30 μm DVB/CAR/PDMS extraction head (Supelco, USA), four-arm olfactometer XL4-30-300 (Nanjing Celai, China), Vacuum pump (Vacuum pump, Tuthill, USA), electroantennal potentiometer EAG (SYNTECH, Germany), etc.
Example 1
Isolation and identification of host plant volatiles
Fresh plants harvested from the field were wrapped around the wound with a moist cotton ball to reduce the escape of volatiles from the wound. Weighing 5.0g of fresh picked sample, placing into a 100mL sampling bottle, sealing, balancing in a water bath at 40 ℃ for 20min, inserting a 50/30 μm DVB/CAR/PDMS extraction head, extracting at 40 ℃ for 40min, and resolving for 3 min. Taking out, immediately inserting into a GC-MS injection port, and extracting volatile substances at the injection port temperature of 250 ℃.
And identifying and analyzing the volatile matters by using a gas chromatography-triple quadrupole mass spectrometer, and searching a spectrum library to be NIST. GC conditions were as follows: DB-5MS column (30m × 0.25mm, 0.25 μm); temperature rising procedure: the initial temperature is 50 ℃, the temperature is kept for 2min, the temperature is increased to 250 ℃ at the speed of 10 ℃/min, the temperature is kept for 35min, and the operation is carried out for 57 min; the carrier gas is high-purity helium (99.999%); the sample is injected without flow splitting, and the flow rate is 1 mL/min. MS conditions: the ion source is an Electron Ionization (EI) source, and the temperature of the ion source is 260 ℃; electron energy 70 eV; the temperature of a sample inlet is 250 ℃; the solvent is delayed for 1min, and the scanning range is 50-500 m/z.
After 5 host plant volatile substances were extracted by GC-MS technique, the separated host plant compounds were identified by computer retrieval technique as shown in FIG. 1. The rape identifies 9 compounds, 9 chenopodium glaucum and 11 cotton; 10 kinds of black nightshade; purslane has a minimum of 6, and a total of 25 compounds have been identified, including alkanes, alcohols, esters, aldehydes, and other classes of compounds. The alkane comprises 5 kinds, 5 kinds of alcohols, 4 kinds of aldehydes, 3 kinds of terpenes, 3 kinds of acids, 2 kinds of esters, 2 kinds of sulfides and 1 kind of ketones. In 5 host plants, 2-hexenal is shared by 4 plants of rape, chenopodium glaucum, purslane and solanum nigrum; 2,6, 10-trimethyltetradecane and ocimene are shared by 3 plants; phenylacetaldehyde, methyl salicylate, 3-hexen-1-ol, cis-2-hexen-1-ol, eicosapentaenoic acid, trans-2-hexen-1-ol, 1-penten-3-ol and 1, 4-pentadiene are shared by two host plants; other compounds not mentioned are unique to each host plant.
The percentage content of each component of the plant volatile matter is calculated, and the result of an area normalization method is shown in table 1.
TABLE 1 types and relative amounts of volatiles released by host plants
Note: a, rape; b, chenopodium glaucum; c, purslane; d, black nightshade; e cotton
In the rape, the relative content of dimethyl trithione reaches 31.49%, the relative content of dimethyl tetrasulfide reaches 23.63%, and the relative content of butyl isothiocyanate reaches 4.12%; the relative content of 2-hexenal in chenopodium glaucum is 38.93%; in cotton, the relative content of beta-pinene reaches 37.48 percent; in the solanum nigrum, the relative content of cis-2-hexene-1-alcohol reaches 10.87 percent; compounds not listed, in a state where their relative contents were all less than 3%, were present in each host plant. The invention selects 6 kinds of odor compounds, purchases standard products, and the detailed information is shown in table 2.
TABLE 2 purity and Source of Standard Compounds
Example 2
EAG response of lygus pratensis to compound standards
The EAG determination method comprises the following steps: sleeving a 6-10-day-old lygus pratensis adult by using a finger-shaped pipe, placing the adult in an ice surface frost halo with the abdomen facing upwards under a stereoscopic microscope, rapidly cutting off the adult by using a double-sided blade along the middle part of the front foot leg section of the lygus pratensis, observing whether a tissue membrane at a separation part overflows a tissue fluid or not, and if the tissue membrane is cracked, preparing again; if the tissue membrane is not ruptured, the next experiment can be performed. Then cutting off a small part of the tips of the two tentacles, slightly inserting the two tentacles by aligning a prepared reference electrode (0.9% NaCl solution is soaked in a capillary tube) with a tissue membrane at the incision, slightly approaching the lower part and not inserting too deeply when the reference electrode is inserted, stopping inserting when slight resistance is sensed, connecting the reference electrode with a head to an EAG micro-operation table, firstly adjusting a knob on the micro-operation table, and using an insect needle to assist in poking one tentacle into the capillary tube of the recording electrode, and then using the insect needle to poke the other tentacle into the capillary tube. The two electrodes are connected by silver wires, and after the steps are carried out, the EAG system is connected.
After the EAG system is communicated, blank control-paraffin oil, internal reference-leaf alcohol are arranged, internal reference and odor standard samples with the concentration of 100 mu L/mL are prepared, and the test is carried out by the dosage of 10 mu L. Each adelphocoris suturalis adult was tested in the order "control-reference-random testing of the remaining standard compounds-reference-control". During the test, the indoor environment is controlled at 25 +/-2 ℃ and the humidity is 60-70%. By the following formula: r ═ volatile reaction value-control reaction value)/(reference reaction value-control reaction value), and R is the EAG reaction relative value of the volatile. The results of the response of the adelphocoris suturalis female and male adults to the antennal potentials of 6 host plant volatile substances are shown in figure 2, wherein the letters a and b in the figure are different and represent that the adelphocoris suturalis has significance at the level of significant difference (P < 0.05) tested by a Duncan's new complex range method.
As can be seen from FIG. 2, there is a difference in EAG response of female and male lygus pratensis to each compound. The highest EAG value is phenylacetaldehyde and the lowest EAG value is linalool. EAG value of female: phenylacetaldehyde > ocimene > methyl salicylate > butyl isothiocyanate > trans-2-hexenol > linalool; male EAG value: methyl salicylate > ocimene > phenylacetaldehyde > trans-2-hexenol > butyl isothiocyanate > linalool. For the same compounds tested, the EAG value of the male worms is smaller than that of the female worms.
Example 3
Olfactory tendency behavior reaction of lygus pratensis to compound standard
The tropism of the adelphocoris suturalis to the adelphocoris suturalis adults to trans-2-hexenol, phenylacetaldehyde, linalool, ocimene, methyl salicylate and butyl isothiocyanate is determined by using a four-arm olfactometer. Folding filter paper strip (0.5cm × 5cm) in half, and dropping 10uL of single component-containing liquidThe concentration of the pure liquid paraffin solution in the test is 100 mu L/mL, and after 30s, the filter paper strip is put into a wide-mouth bottle to be used as an odor source, and the liquid paraffin is used as a solvent and a control. And releasing the single lygus pratensis adults to the insect placing holes, timing when the single lygus pratensis adults are positioned in the central area, and observing the behavioral response of the lygus pratensis adults within 5 min. The lygus pratensis is placed in the central circle, and if adults cross a certain arm for 3cm and stay in the area of the arm for more than 5s, the adults are considered to be attracted by the odor source in the direction to generate a tendency behavior. The adult insects are tested once, the hose is changed by exchanging gas for 10 times of test, and the host plants are changed for 20 times of test. Each group of treatments tested the adult male and female lygus pratensis at 60 heads, and the adult lygus pratensis was starved for 5h before use in the experiment. After the test, the four-arm olfactometer, the gas washing bottle and the connecting rubber tube are cleaned by ethanol. Chi 2 was used to test whether adult lygus pratensis selected for hypothesis H between the two taste sources 0 For a 50:50 theoretical distribution, the χ 2 value and the corresponding significance level P value were calculated.
The formula I is as follows: the luring rate is treated insect number/tested total insect number multiplied by 100%
The formula II is as follows: the avoidance rate is that the number of the control insects/the total number of the tested insects is multiplied by 100 percent
The formula III is as follows: the reaction rate is (treated worm number + control worm number)/total worm number tested x 100%
The results of the behavioral responses of females and males to 6 plant volatiles are shown in tables 3 and 4. The experimental data were statistically analyzed using SPSS22.0 statistical software.
Table 3 behavioral response of lygus pratensis females to 6 plant volatiles
Note: saliency represents P < 0.05, represents P < 0.01, and ns represents P > 0.05.
TABLE 4 behavioral response of Nephocoris pratensis on volatile substances of 6 host plants
Note: in significance ns means P > 0.05.
The trending behavior of lygus pratensis suggests that females are more susceptible to volatile attraction than males under the same compounds. According to the analysis of a chi 2 test, the behavioral response of phenylacetaldehyde to female insects shows very obvious difference (P is less than 0.01), and the behavioral response of phenylacetaldehyde to butyl isothiocyanate shows obvious difference (P is less than 0.05); and the behavioral response differences of the lygus pratensis male insect on 6 compounds of trans-2-hexenol, phenylacetaldehyde, linalool, ocimene, methyl salicylate and butyl isothiocyanate are not obvious.
According to the invention, active substances are determined by comparing GC-MS graphs, a spectrum library is searched to be NIST, and after the active substances are identified and screened, the taxis response of the lygus pratensis to volatile substances of various host plants is researched. The results show that after GC-MS identification, 9 compounds, 9 chenopodium glaucum, 11 cotton, 10 black nightshade herbs and 6 purslane herbs are identified from rape. Through literature reference and combination of early-stage research results of a subject group, 6 host plant volatile substances are screened to determine EAG and tendency behavior reactions of lygus pratensis, and the results show that the EAG reactions of female and male lygus pratensis to various compounds are different, and the EAG value of male lygus pratensis is smaller than that of female lygus pratensis. The highest EAG value is phenylacetaldehyde and the lowest EAG value is linalool. EAG value of female: phenylacetaldehyde > ocimene > methyl salicylate > butyl isothiocyanate > trans-2-hexenol > linalool; the differences in EAG values between males and individual compounds were not significant. The behavioral response of phenylacetaldehyde to female insects is remarkably different (P is less than 0.01), and the behavioral response of phenylacetaldehyde to butyl isothiocyanate is remarkably different (P is less than 0.05); the differences in the behavioral responses of males to 6 compounds were not significant.
According to the method, the adelphocoris suturalis female is obtained to have obvious selectivity on phenylacetaldehyde by combining the EAG and the behavior reaction of the adelphocoris suturalis to 6 compounds, the compound capable of regulating and controlling the tendency behavior of the adelphocoris suturalis a potential candidate component for configuring an attractant for the adelphocoris suturalis female, and a regulating and controlling agent for the tendency behavior of the adelphocoris suturalis female can be designed to be used for the measurement and prevention and control of the adelphocoris suturalis female.
Although the invention has been described in detail with respect to the general description and the specific embodiments, it will be apparent to those skilled in the art that modifications and improvements may be made based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (6)
1. The application of the lygus pratensis host plant volatile matters in controlling lygus pratensis is characterized in that the lygus pratensis host plant volatile matters comprise any one or more of phenylacetaldehyde, ocimene, methyl salicylate, butyl isothiocyanate, trans-2-hexenol and linalool.
2. Use according to claim 1, characterized in that phenylacetaldehyde and/or butyl isothiocyanate is used for controlling lygus pratensis females.
3. Use according to claim 1, characterized in that phenylacetaldehyde is used for controlling lygus pratensis females.
4. Use according to any one of claims 1 to 3, wherein the phenylacetaldehyde is present in a concentration of 100 ± 10 μ L/mL.
5. The use according to any one of claims 1 to 3, wherein the concentration of butyl isothiocyanate is 100 ± 10 μ L/mL.
6. Use of the lygus pratensis host plant volatile of claim 1 in the preparation of a lygus pratensis attractant.
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| NL2033791A NL2033791B1 (en) | 2022-06-10 | 2022-12-22 | Host plant volatile of l.pratensis |
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| CN110100818B (en) * | 2019-05-31 | 2021-05-11 | 塔里木大学 | Lygus pratensis attractant and preparation method and application thereof |
| CN112335654B (en) * | 2019-08-08 | 2022-03-18 | 中国农业科学院植物保护研究所 | Composition and attractant for attracting red-neck common-room hornets, and preparation and application thereof |
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