CN110100678B - Prevention and treatment method for potato late blight - Google Patents

Prevention and treatment method for potato late blight Download PDF

Info

Publication number
CN110100678B
CN110100678B CN201910357207.7A CN201910357207A CN110100678B CN 110100678 B CN110100678 B CN 110100678B CN 201910357207 A CN201910357207 A CN 201910357207A CN 110100678 B CN110100678 B CN 110100678B
Authority
CN
China
Prior art keywords
late blight
potato late
potato
seed dressing
agent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201910357207.7A
Other languages
Chinese (zh)
Other versions
CN110100678A (en
Inventor
张博
齐军山
张悦丽
马立国
祁凯
李长松
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institute of Plant Protection Shandong Academy of Agricultural Sciences
Original Assignee
Institute of Plant Protection Shandong Academy of Agricultural Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institute of Plant Protection Shandong Academy of Agricultural Sciences filed Critical Institute of Plant Protection Shandong Academy of Agricultural Sciences
Priority to CN201910357207.7A priority Critical patent/CN110100678B/en
Publication of CN110100678A publication Critical patent/CN110100678A/en
Application granted granted Critical
Publication of CN110100678B publication Critical patent/CN110100678B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/06Coating or dressing seed
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/25Root crops, e.g. potatoes, yams, beet or wasabi
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Plant Pathology (AREA)
  • Soil Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Botany (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The invention belongs to the technical field of agricultural pest control, and particularly relates to a control method of potato late blight. According to the method for preventing and treating the potato late blight, the seed dressing agent which comprises the flax lignans, the bacterial cellulose and the spirulina polysaccharides as active ingredients is used for dressing the potato seeds, and the good biological bacteriostatic performance of the seed dressing agent is utilized, so that the efficient bacteriostatic treatment can be performed on the potato seeds at the beginning of planting, the potato late blight can be effectively prevented and treated, the field prevention and control effect is good, and the potato late blight is safe to test crops in the application range.

Description

Prevention and treatment method for potato late blight
Technical Field
The invention belongs to the technical field of agricultural pest control, and particularly relates to a control method of potato late blight.
Background
Potato Late Blight (Potato Late Blight) is a devastating oomycete disease caused by phytophthora infestans that results in death of Potato stems and leaves and rot of tubers. When the leaves are infected, green brown spots are soaked in water at the leaf tips or leaf margins, the peripheries of the infected spots are provided with light green halos, the infected spots are rapidly enlarged and brown when the humidity is high, a circle of white mould, namely cyst stalks and sporangia, is most obvious particularly on the leaf backs, the infected spots become brown and dry when being dried, are crisp and easy to crack, do not see white mould, and have slow expansion speed, and brown streak spots appear when the stems or leaf stalks are infected; the seriously ill leaves can wither and contract, so that the whole plant is black and rotten, even one scorched field emits putrefactive odor; the tubers are infected by brown or purple brown large disease spots, are slightly sunken, and the subcutaneous potato flesh at the diseased part is brown and slowly expands or is rotten all around.
The pathogenic bacteria of the potato late blight mainly live through the winter in potato blocks by mycelium, and the potato blocks with the pathogenic bacteria are sown, so that the potato blocks do not germinate or die after germination and unearthed, some unearthed potato blocks become central diseased plants, and sporangiums generated in diseased parts are re-infected by airflow propagation to form a diseased center, so that the disease is spread and expanded rapidly from point to surface; the sporangia on the diseased leaves can infiltrate into soil along with rainwater or irrigation water to infect potato blocks to form diseased potatoes, and the diseased potatoes become a main infection source in the next year. Therefore, the potato late blight has great harm to the planting of the potatoes.
The method for preventing and treating potato late blight in the prior art mainly comprises the following steps: 1. selecting disease-free seed potatoes to reduce initial infection sources, strictly removing the disease potatoes each time in the processes of autumn harvest and cellar entry, winter storage and cellar checking, cellar exit, dicing, vernalization and the like, conditionally establishing a disease-free seed reserving land and performing disease-free seed reserving; 2. the cultivation management is enhanced, the seedlings are sowed early in a proper period, the field with loose soil and good drainage is selected for planting, the field humidity is reduced, the robust growth of plants is promoted, and the disease resistance is enhanced; 3. crop rotation and stubble replacement are performed to prevent continuous cropping and continuous cropping with solanaceae crops or close to planting, crop rotation with cruciferous vegetables is performed for more than 3 years, and adjacent planting with potatoes is avoided; 4. strengthening field management, applying enough base fertilizer, applying formula fertilizer, avoiding applying nitrogen fertilizer partially, applying phosphorus and potassium fertilizers additionally, preventing and removing weeds in time after field planting, reasonably training branches, pinching and branching according to the fruiting habits of different varieties, reducing nutrient consumption and promoting the growth of main stems; 5. reasonable close planting, wherein different close planting modes are adopted according to the growth period and fruiting habit of different varieties, so that the ventilation and light transmission conditions in the field can be improved, the field humidity is reduced, and the occurrence of diseases is reduced; 6. sterilizing the potato seeds, and optionally sterilizing with proper disinfectant; 7. in the early disease stage, proper medicament can be selected for controlling the disease in the early disease stage of the plant. In the control method, the seeds are correspondingly subjected to bacteriostatic treatment in the early planting stage, so that the control method has a remarkable effect on controlling the potato late blight, and the development of the method for efficiently controlling the potato late blight has important significance on potato planting.
Disclosure of Invention
Therefore, the technical problem to be solved by the invention is to provide a method for preventing and treating potato late blight.
In order to solve the technical problems, the method for preventing and treating the potato late blight comprises the steps of carrying out seed dressing treatment on a seed dressing agent for preventing and treating the potato late blight and potato seeds during sowing;
the effective components of the seed dressing agent comprise the following components in a mass ratio of 1-3: 3-8 of flax lignan and bacterial cellulose.
Preferably, the effective components of the seed dressing agent comprise the following components in a mass ratio of 2: 5, and bacterial cellulose.
Preferably, the effective component of the seed dressing agent also comprises spirulina polysaccharide.
Specifically, the mass ratio of the flax lignans to the bacterial cellulose to the spirulina polysaccharide is 1-3: 3-8: 2-4.
Specifically, the mass ratio of the flax lignans to the bacterial cellulose to the spirulina polysaccharide is 2: 5: 3.
preferably, the mass content of the effective components in the seed dressing agent is not less than 20 wt%.
Preferably, the seed dressing agent is used in an amount of 1-3 wt% of the weight of the potato seeds based on the amount of the effective ingredients.
Specifically, the seed dressing agent comprises a water suspending agent and water dispersible granules.
In the case of water dispersible granules, those skilled in the art use the following major adjuvants and formulate them by conventional methods: the dispersant is selected from polycarboxylate, lignosulfonate, alkyl naphthalene sulfonate and NNO (alkyl naphthalene sulfonate formaldehyde condensate); the wetting agent is selected from polyoxyethylene alcohol, alkyl sulfate, alkyl sulfonate and naphthalene sulfonate; the disintegrating agent is selected from ammonium sulfate, urea, sucrose, glucose, citric acid, succinic acid, sodium bicarbonate, nekal BX (sodium dibutylnaphthalenesulfonate), and K-12 (sodium laurylsulfate); the binder is selected from diatomite, corn starch, PVA, carboxymethyl (ethyl) cellulose and microcrystalline cellulose; the filler is selected from ammonium sulfate, diatomaceous earth, kaolin, white carbon black, light calcium, sepiolite, talcum powder, attapulgite, pottery clay and the like.
The preparation of the water dispersible granules can be carried out by methods known to those skilled in the art, such as: according to the formula proportion, the raw medicine is uniformly mixed with a dispersing agent, a wetting agent, a binder, a filler and the like, powder is obtained after airflow crushing, a proper amount of water phase is added for mixing, granulation is carried out in a granulator, and then drying and screening are carried out, so that the water dispersible granule is obtained.
For aqueous suspensions, the skilled worker uses the following essential auxiliaries and formulates them by conventional methods: the dispersant is selected from polycarboxylate, lignosulfonate, alkylnaphthalene sulfonate, TERSPERSE 2425 (alkylnaphthalene sulfonate, available from Hensman, USA); the emulsifier is selected from Nongru 700# (common name: alkylphenol formaldehyde resin polyoxyethylene ether), Nongru 2201, span-60 # (common name: sorbitan monostearate), emulsifier T-60 (common name: sorbitan monostearate polyoxyethylene ether), Nongru 1601# (common name: phenethylphenol polyoxyethylene polyoxypropylene ether), TERSPERSE 4894 (product of Hensmei company in USA) and FS3000 (phosphate ester type anionic surfactant); the wetting agent is selected from alkylphenol polyoxyethylene polyether formaldehyde condensate sulfate, alkylphenol polyoxyethylene ether phosphate, phenethylphenol polyoxyethylene ether phosphate, alkyl sulfate, alkyl sulfonate, naphthalene sulfonate, TERSPERSE 2500 (available from Henshime company, USA), SXC (sulfonic acid type anionic surfactant); the thickener is selected from xanthan gum, polyvinyl alcohol, magnesium aluminum silicate, and bentonite; the preservative is selected from benzoic acid, sodium benzoate, BIT (1, 2-benzisothiazolin-3-one); the defoaming agent is an organic silicon defoaming agent; the antifreeze is selected from ethylene glycol, propylene glycol, glycerol, and urea.
The preparation of the aqueous suspension may be carried out by methods known to the person skilled in the art, for example: according to the formula proportion, water is used as a medium, the original drug, the dispersing agent, the suspending agent, the antifreezing agent and the like are added into a high-shear emulsifying machine for shearing, and then a sand mill is used for sanding to obtain the suspending agent.
Preferably, the method for preventing and treating potato late blight further comprises the step of drying the seed-dressing treated potato seeds in the shade.
The invention also discloses application of the method for preventing and treating the potato late blight in the field of prevention and treatment of the potato late blight.
According to the method for preventing and treating the potato late blight, the seed dressing agent which comprises the flax lignans, the bacterial cellulose and the spirulina polysaccharides as active ingredients is used for dressing the potato seeds, and the good biological bacteriostatic performance of the seed dressing agent is utilized, so that the efficient bacteriostatic treatment can be performed on the potato seeds at the beginning of planting, the potato late blight can be effectively prevented and treated, the field prevention and control effect is good, and the potato late blight is safe to test crops in the application range.
Detailed Description
Example 1
The seed dressing agent for preventing and treating potato late blight, which is described in the embodiment, comprises the following effective components in mass ratio of 2: 5, and bacterial cellulose.
The seed dressing agent of the embodiment is prepared by taking flax lignan and bacterial cellulose as effective components, selecting a proper auxiliary agent and a proper filler to be uniformly mixed according to a conventional method in the prior art, performing air flow crushing to obtain powder, adding a certain amount of water to mix, granulating in a granulator, and drying and screening to obtain granular water dispersible granules, wherein the mass content of the effective components in the seed dressing agent is controlled to be 20 wt%.
Example 2
The seed dressing agent for preventing and treating potato late blight, which is described in the embodiment, comprises the following effective components in mass ratio of 3: 3, and bacterial cellulose.
The seed dressing agent of the embodiment is prepared by taking flax lignan and bacterial cellulose as effective components, selecting a proper auxiliary agent and a proper filler to be uniformly mixed according to a conventional method in the prior art, performing air flow crushing to obtain powder, adding a certain amount of water to mix, granulating in a granulator, and drying and screening to obtain granular water dispersible granules, wherein the mass content of the effective components in the seed dressing agent is controlled to be 30 wt%.
Example 3
The seed dressing agent for preventing and treating potato late blight, which is described in the embodiment, comprises the following effective components in mass ratio of 1: 8, secoisolariciresinol diglucoside and bacterial cellulose.
The seed dressing agent of the embodiment is prepared by taking flax lignan and bacterial cellulose as effective components, selecting a proper auxiliary agent and a proper filler to be uniformly mixed according to a conventional method in the prior art, performing air flow crushing to obtain powder, adding a certain amount of water to mix, granulating in a granulator, and drying and screening to obtain granular water dispersible granules, wherein the mass content of the effective components in the seed dressing agent is controlled to be 40 wt%.
Example 4
The seed dressing agent for preventing and treating potato late blight, which is described in the embodiment, comprises the following effective components in mass ratio of 2: 5: 3, flax lignan, bacterial cellulose and spirulina polysaccharide.
In the bactericide of this embodiment, the flax lignan, the bacterial cellulose and the spirulina polysaccharide are used as effective components, water is used as a medium according to a conventional method in the prior art, and the active components, the dispersing agent, the suspending agent, the antifreezing agent and the like are added into a high shear emulsifying machine to be sheared for 30min, and then the mixture is sanded by a sand mill to obtain the water suspending agent. In this example, the mass content of the above effective components in the seed dressing is controlled to be 25 wt%.
Example 5
The seed dressing agent for preventing and treating potato late blight, which is described in the embodiment, comprises the following effective components in mass ratio of 1: 3: 2, secoisolariciresinol diglucoside, bacterial cellulose and spirulina polysaccharide.
In the bactericide of this embodiment, the flax lignan, the bacterial cellulose and the spirulina polysaccharide are used as effective components, water is used as a medium according to a conventional method in the prior art, and the active components, the dispersing agent, the suspending agent, the antifreezing agent and the like are added into a high shear emulsifying machine to be sheared for 30min, and then the mixture is sanded by a sand mill to obtain the water suspending agent. In this example, the mass content of the above effective components in the seed dressing is controlled to be 35 wt%.
Example 6
The seed dressing agent for preventing and treating potato late blight, which is described in the embodiment, comprises the following effective components in mass ratio of 3: 8: 4 flax lignan, bacterial cellulose and spirulina polysaccharide.
In the bactericide of this embodiment, the flax lignan, the bacterial cellulose and the spirulina polysaccharide are used as effective components, water is used as a medium according to a conventional method in the prior art, and the active components, the dispersing agent, the suspending agent, the antifreezing agent and the like are added into a high shear emulsifying machine to be sheared for 30min, and then the mixture is sanded by a sand mill to obtain the water suspending agent. In this example, the mass content of the above effective components in the seed dressing is controlled to be 30 wt%.
Examples of the experiments
1. Biological assay
In the experimental example, a method of the agricultural industry standard NY/T1156.2-2006 of the people's republic of China is adopted, a test object is a commercial potato late blight pathogenic bacterium Phytophthora infestans (Mont.) de Bary purchased from Zhongnong Hua Biotech Co., Ltd, and a medicine-containing culture medium method is adopted: 6mL of liquid medicine with serial concentrations (shown in the following table 1) obtained by compounding each single agent and each effective component is respectively added into 54mL of PDA culture medium cooled to 45 ℃ to prepare a medicine-containing culture medium plate with the required final concentration, and the medicine-free plate is used as a control. Then, 6mm diameter hypha blocks are prepared from the edges of the pathogenic bacteria colony cultured for 7 days, and the hypha blocks are moved to each series of drug-containing culture media with the hypha facing downwards, and each treatment is repeated for 4 times. After the treatment, the cells are placed in a constant-temperature biochemical incubator at 25 +/-1 ℃ for dark culture, the diameter of the bacterial colony is measured after the test treatment is carried out for 3d, the average value of 4 times of repetition is obtained, namely the average bacterial colony diameter, the growth diameter of the treated average bacterial colony is obtained, namely the average bacterial colony diameter-5 mm, the inhibition rate of the effective components compounded in each proportion is calculated according to the following formula, and the inhibition rate is recorded in the following table 1.
Figure BDA0002045782350000071
TABLE 1 test agent concentration setting protocol and inhibition ratio results (%)
Agents/concentrations (mg/L) 10mg/L 20mg/L 30mg/L
Flax lignan (A) 18.03% 21.45% 24.15%
Bacterial cellulose (B) 28.88% 36.75% 45.12%
Spirulina polysaccharide (C) 10.34% 14.23% 19.72%
A:B=2:5 62.61% 75.35% 87.15%
A:B=3:3 55.14% 65.23% 78.73%
A:B=1:8 59.33% 70.82% 81.11%
A:B:C=2:5:3 71.03% 83.55% 96.87%
A:B:C=1:3:2 65.13% 77.25% 90.38%
A:B:C=3:8:4 68.98% 81.21% 94.05%
The data in the table show that the biological agent prepared by taking the flax lignan, the bacterial cellulose and the spirulina polysaccharide as active ingredients has good bacteriostatic activity on the isolated pathogenic bacteria of the potato late blight.
2. Field test
The method comprises the steps of taking potato seeds to be planted, cutting the potato seeds into blocks for standby, carrying out conventional seed dressing treatment according to the mixing ratio of the following medicament (calculated by effective components) and seeds, adding water into the effective components in each ratio for mixing during seed dressing, then carrying out seed dressing treatment, then airing in the absence of sunlight, sowing, and taking the condition that the seed dressing treatment is not carried out under the same culture condition as a contrast. The incidence (%) of the plants in the field was investigated 30 days after sowing.
The morbidity and control effect under the compounding of each proportion are counted and recorded in the following table 2.
Table 2 test agent concentration setting protocol and control results (%)
Figure BDA0002045782350000072
Figure BDA0002045782350000081
As can be seen from the field control test results, the bactericide disclosed by the invention takes the flax lignans, the bacterial cellulose and the spirulina polysaccharide as active ingredients, can effectively control the attack of the potato late blight, has a good field control effect, and is safe to test crops in a pesticide application range.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.

Claims (10)

1. A method for preventing and treating potato late blight is characterized by comprising the step of carrying out seed dressing treatment on a seed dressing agent for preventing and treating potato late blight and potato seeds during sowing;
the effective components of the seed dressing agent comprise the following components in a mass ratio of 1-3: 3-8 of flax lignan and bacterial cellulose.
2. The method for controlling potato late blight according to claim 1, wherein the effective ingredients of the seed dressing agent comprise, by mass, 2: 5, and bacterial cellulose.
3. The method for controlling potato late blight according to claim 1 or 2, wherein the effective ingredient of the seed dressing agent further comprises spirulina platensis polysaccharide.
4. The method for controlling potato late blight according to claim 3, wherein the mass ratio of the flax lignan to the bacterial cellulose to the spirulina polysaccharide is 1-3: 3-8: 2-4.
5. The method for preventing and treating potato late blight according to claim 4, wherein the mass ratio of the flax lignans to the bacterial cellulose to the spirulina polysaccharide is 2: 5: 3.
6. the method for controlling potato late blight according to any one of claims 1 to 5, wherein the seed dressing agent contains the active ingredient in an amount of not less than 20 wt% by mass.
7. The method for controlling potato late blight according to any one of claims 1 to 6, wherein the seed dressing agent is used in an amount of 1 to 3 wt% based on the mass of the potato seeds, based on the amount of the active ingredient.
8. The method for controlling potato late blight according to any one of claims 1 to 7, wherein the seed dressing agent comprises an aqueous suspension agent, water dispersible granules.
9. The method for controlling potato late blight according to any one of claims 1 to 8, further comprising a step of drying the seed-dressing-treated potato seeds in the shade.
10. Use of the method for controlling potato late blight according to any one of claims 1 to 9 in the field of control of potato late blight.
CN201910357207.7A 2019-04-29 2019-04-29 Prevention and treatment method for potato late blight Active CN110100678B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910357207.7A CN110100678B (en) 2019-04-29 2019-04-29 Prevention and treatment method for potato late blight

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201910357207.7A CN110100678B (en) 2019-04-29 2019-04-29 Prevention and treatment method for potato late blight

Publications (2)

Publication Number Publication Date
CN110100678A CN110100678A (en) 2019-08-09
CN110100678B true CN110100678B (en) 2021-09-14

Family

ID=67487625

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201910357207.7A Active CN110100678B (en) 2019-04-29 2019-04-29 Prevention and treatment method for potato late blight

Country Status (1)

Country Link
CN (1) CN110100678B (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103467463A (en) * 2013-09-18 2013-12-25 南开大学 Lignin derivatives as well as preparation method and use thereof
CN107821455A (en) * 2017-10-16 2018-03-23 湖南农业大学 A kind of tobacco health upgrading Chinese medicine preparation and its application method
CN108244150A (en) * 2017-12-18 2018-07-06 广州美林生态科技有限公司 Novel crops insect prevention, mould proof, expelling parasite lotion and its preparation method and application
CN109152367A (en) * 2016-02-09 2019-01-04 巴斯夫欧洲公司 Mixture and composition comprising bacillus genus bacterial strain or its metabolin and other biological pesticides

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105315118A (en) * 2015-07-10 2016-02-10 李玉山 Integrated extracting and separating method for active ingredients of Chinese stellera roots

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103467463A (en) * 2013-09-18 2013-12-25 南开大学 Lignin derivatives as well as preparation method and use thereof
CN109152367A (en) * 2016-02-09 2019-01-04 巴斯夫欧洲公司 Mixture and composition comprising bacillus genus bacterial strain or its metabolin and other biological pesticides
CN107821455A (en) * 2017-10-16 2018-03-23 湖南农业大学 A kind of tobacco health upgrading Chinese medicine preparation and its application method
CN108244150A (en) * 2017-12-18 2018-07-06 广州美林生态科技有限公司 Novel crops insect prevention, mould proof, expelling parasite lotion and its preparation method and application

Also Published As

Publication number Publication date
CN110100678A (en) 2019-08-09

Similar Documents

Publication Publication Date Title
CN106818875B (en) Fruit tree root rot control agent and method
CN111109023B (en) Method for crop rotation and intercropping of 3 crops of sugarcane, silage corn and millet
CN110616172B (en) Bacillus with crucifer clubroot prevention and treatment function and preparation method and application thereof
CN107836314A (en) A kind of Cultivation of High Yield Peanuts method for releasing continuous cropping obstacle
CN104839180B (en) Fungicide composition and application thereof
EA039311B1 (en) Pesticide composition containing thidiazuron and polyglutamic acid
CN114605203A (en) Compound microbial fertilizer and application thereof
CN110651651A (en) Method for preventing and treating tomato stem rot and root rot diseases
CN110100678B (en) Prevention and treatment method for potato late blight
CN1154786A (en) Non-traditional high-yield cultivation technology for interplanting maize in wheat field
CN114617127B (en) Pesticide sterilization composition and application thereof
CN105475051A (en) Method for preventing radix psammosilenes root rot disease
CN110100830B (en) Bactericide for preventing and treating potato late blight and preparation method thereof
RU2481760C2 (en) Method of plant cultivation
CN104973994B (en) A kind of anti-continuous cropping disease organic fertilizer of cucumber
CN113349223A (en) Plant microorganism seed dressing agent and use method thereof
CN107006159A (en) A kind of method of industrialized agriculture secondary salinization soil reparation improvement
CN106588508B (en) Special fertilizer for preventing and treating clubroot of cruciferous crops as well as preparation method and application thereof
CN105394067A (en) Suspended seed coating containing thifluzamide, metalaxyl and clothianidin
CN102515973B (en) Method for producing organic fertilizer for controlling soil meloidogyne
CN112674103A (en) Antibacterial composition, pesticide bactericide and method for preventing and treating diseases of crops
CN108812700A (en) A kind of granule containing Zhongshengmycin and bacillus subtilis
CN110100821A (en) A kind of biological agent and the preparation method and application thereof for preventing and treating the late blight of potato
CN105061036B (en) The biological organic fertilizer produced using mushroom mushroom tang and tobacco stems and its application
CN108934823A (en) A method of cultivating watermelon

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant