CN110095456A - A kind of tobacco virus detection display and application method - Google Patents
A kind of tobacco virus detection display and application method Download PDFInfo
- Publication number
- CN110095456A CN110095456A CN201910292423.8A CN201910292423A CN110095456A CN 110095456 A CN110095456 A CN 110095456A CN 201910292423 A CN201910292423 A CN 201910292423A CN 110095456 A CN110095456 A CN 110095456A
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- shell
- tobacco
- needle
- detection
- virus
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Abstract
The invention discloses a kind of tobacco virus detection display and application methods, comprising: several extraction needles of setting in shell and the housing accommodating chamber；The housing cross-sectional is cirque structure, the shell of cirque structure is the connection of at least two semicircular structures, the shell of two semicircular structures is connected by shaft, sealing film is equipped at the tip inlets for extracting needle, the internal ring wall of the shell is run through at the tip for extracting needle, central point of the tip towards the shell, the extraction needle is double-deck pipe body, described inner tube one end for extracting needle connects described outer tube one end for extracting needle, said inner tube free end connects piston, and the outer tube accommodating chamber is provided with the detection agent of detection virus；When using display is detected, the shell is inserted in and is fixed in tobacco stalk；Inside the tip insertion tobacco stalk crust for extracting needle；Tobacco juice enters said inner tube, is entered in the outer tube accommodating chamber by the free end of said inner tube, mixes with the detection agent of detection virus, through the variation of shell observation detection agent.
Description
Technical field
The present invention relates to phytopathogen Prevention Technique more particularly to a kind of tobacco virus detection display and users
Method.
Background technique
Tobacco virus is that the major class disease most wide, generation is the most universal is distributed on current tobacco leaf production.Currently, China
It has been found that tobacco virus have 16 kinds, wherein be led to tobacco mosaic disease virus mainly have 3 kinds of viruses such as TMV, CMV, PVY.
Most area virosis is with several viruses such as tobacco cucumber mosaic virus sick (CMV), tobacco mosaic viruses sick (TMV)
Disease mixing occurs, superinfection.Field diseased plant rate generally 20%~40%, weight up to 40%~80% (some local plot
Up to 100%).After Tobacco Infected virus, chlorophyll is damaged, and photosynthesis weakens, and leaf growth is suppressed, and leaf is small, lopsided,
Underproduction amplitude is up to 20%~80%.After virosis occurs, the quality of tobacco leaf is also seriously affected, quality is deteriorated.
Tobacco virus causes the tobacco underproduction to be reduced, reduces simultaneously because it is found that will lead to large area Tobacco Infected not in time
The quality of tobacco, at present tobacco virus detection cannot accomplish to detect in viral initial phase as a result, miss the best prevention and treatment time,
It cannot be detected at any time during plant strain growth, not accomplish to monitor constantly.
Summary of the invention
The present invention overcomes the deficiencies in the prior art, a kind of tobacco virus detection display and application method are provided.
In order to achieve the above objectives, the first technical solution that the present invention uses are as follows: a kind of tobacco virus detection display,
It include: several extraction needles of setting in shell and the housing accommodating chamber, which is characterized in that the housing cross-sectional is circle
Ring structure, the shell of cirque structure are the connection of at least two semicircular structures, the shell of two semicircular structures
Body is connected by shaft, sealing film is equipped at the tip inlets for extracting needle, the tip for extracting needle is through the shell
Internal ring wall, the tip is towards the central point of the shell, and the extraction needle is double-deck pipe body, described inner tube one end for extracting needle
Described outer tube one end for extracting needle is connected, said inner tube free end connects piston, and the outer tube accommodating chamber is provided with detection virus
Detection agent.
In a preferred embodiment of the present invention, the inspection for the detection virus being equipped in the outer tube receiving cavity of several extraction needles
It is different to survey agent.
In a preferred embodiment of the present invention, the piston connects traction element.
In a preferred embodiment of the present invention, the traction element is through the blunt end for extracting needle, the cross of the blunt end
Section is circular configuration, and the cross section of the blunt end is greater than the cross section at the tip, and the cross section at the tip is ring-dot type
Structure.
In a preferred embodiment of the present invention, the piston can slide in said inner tube accommodating chamber.
In a preferred embodiment of the present invention, the piston is trapezium structure, the big one end connection in the piston cross-section face
Sealing ring is arranged in one end of traction element, the traction element at the extraction needle blunt end.
In a preferred embodiment of the present invention, at least one handle is connected on the shell.
In a preferred embodiment of the present invention, fixation member is arranged in the enclosure interior, is capable of fixing the extraction needle, makes
It is more firm in insertion plant to extract needle, the fixation member is to be flexibly connected with the extraction needle, can facilitate replacement shell
The extraction needle in internal portion.
Second of technical solution that the present invention uses are as follows: a kind of application method of tobacco virus detection display, including
Following steps:
(1) shell is inserted in and is fixed in tobacco stalk；
(2) inside the tip insertion tobacco stalk crust for extracting needle；
(3) tobacco juice enters said inner tube, is entered in the outer tube accommodating chamber by the free end of said inner tube, with detection
The detection agent mixing of virus, through the variation of shell observation detection agent.
In a preferred embodiment of the present invention, the skin free edge of two semicircular structures can be socketed, and adjust inner ring
The size of area and perimeter, the rotation of handle one end connect the shell of one of semicircular structure, and the free end of handle can
It is inserted on the shell of another semicircular structure, handle is connected the shell of two semicircular structures.
In a preferred embodiment of the present invention, the shell has material composition flexible, and it is curved to can produce deformation generation
Song can carry out fitting winding around the shape of tobacco stalk plant, and the structure for forming helix is wrapped in the plant of different thicknesses
On.
In a preferred embodiment of the present invention, the sealing film for extracting needle tip opening is release membranes, described
Sealing film can be melted in tobacco stalk juice, and juice, which flows into, after the sealing film contact juice melts extracts needle inner tube.
In a preferred embodiment of the present invention, the shell is transparent shell, and the extraction needle wall is transparent wall, is drawn
Dynamic traction element, makes traction element be detached from said inner tube, extracts the juice of tobacco stalk, transparent shell and transparent extraction needle
The tobacco juice and the mixed phenomenon of the intracorporal detection agent of outer tube accommodating chamber of inner tube extraction can be observed.
In a preferred embodiment of the present invention, the piston is detached from said inner tube free end, in said inner tube accommodating chamber
Air pressure is less than the air pressure outside the extraction needle, and the juice in tobacco stalk sucks inner tube, enters outer tube capacity by inner tube free end
It receives in cavity, juice and detection liquid mixing are detected.
In a preferred embodiment of the present invention, the tobacco juice mixes unconverted for health plant with viral diagnosis agent
Strain, generating discoloration or precipitating is plant in spite of illness.
The invention solves the defect existing in the background technology, the present invention have it is following the utility model has the advantages that
(1) present invention effectively detects tobacco virus, and can clearly observe testing result, detection device
Structure is simple, easy to carry, and production cost is low, and application method is simple, and detection speed is fast, improves the prevention and treatment speed of tobacco virus
Degree, prevents the large area of virosis to infect.
(2) needle is extracted in the present invention and realizes self-priming plant juice using pressure difference, and device environmental protection is pollution-free, being capable of basis
The different growth time section of tobacco is detected.
(3) shell can adjust the compactness of the two according to tobacco plant in the present invention, and shell made of flexible material subtracts
Few scratch injury of the shell to plant epidermis, the mode of spiral winded type can be detected (especially edge to plant body many places
The detection of plant axial direction or length direction), and plant stalk can be made to grow vertically, play fixed function.
(4) handle detection display easy to carry can fix shell when carrying out virosis detection in the present invention
On plant body.
(5) inventive shell and extraction needle are that transparent material is made, mixed convenient for observation tobacco juice and viral diagnosis agent
The variation for closing liquid, capable of quickly judging plant, whether virus infection is sick, keeps observation more intuitive.
(6) the extraction needle inside inventive shell can be replaced, and opening fixation member will test used mention
It takes needle to take out, is replaced with new extraction needle, is immobilized in the shell, flexible choice
Detailed description of the invention
Present invention will be further explained below with reference to the attached drawings and examples；
Fig. 1 is the three-dimensional structure diagram of the preferred embodiment of the present invention；
Fig. 2 is the three-dimensional structure diagram of the preferred embodiment of the present invention；
Fig. 3 is the three-dimensional structure diagram of the preferred embodiment of the present invention；
Fig. 4 is the three-dimensional structure diagram of the preferred embodiment of the present invention；
Fig. 5 is the three-dimensional structure diagram of the preferred embodiment of the present invention；
Fig. 6 is the three-dimensional structure diagram of the preferred embodiment of the present invention；
In figure: shell 1；Extract needle 2；Handle 3；Shaft 4；Inner tube 5；Piston 6；Traction element 7.
Specific embodiment
Presently in connection with drawings and examples, the present invention is described in further detail, these attached drawings are simplified signal
Figure, the basic structure of the invention will be illustrated schematically only, therefore it only shows the composition relevant to the invention.
It should be noted that it can directly on the other part when component is referred to as " being fixed on " another component
Or there may also be components placed in the middle.When a component is considered as " connection " another component, it, which can be, is directly connected to
To another component or it may be simultaneously present component placed in the middle.Term as used herein "upper", "lower", "inner", "outside",
"left", "right" and similar statement for illustrative purposes only, are not meant to be the only embodiment.
As shown in figures 1 to 6, a kind of tobacco virus detects display, comprising: setting in shell 1 and 1 accommodating chamber of shell
Several extract needle 2, which is characterized in that 1 cross section of shell is cirque structure, and the shell 1 of cirque structure is at least two
Semicircular structure connection, the shell 1 of two semicircular structures are connected by shaft 4, are extracted and are equipped with sealing at the tip inlets of needle 2
The internal ring wall of shell 1 is run through at film, the tip for extracting needle 2, and towards the central point of shell 1, extraction needle 2 is double-deck pipe body at tip, is mentioned
5 one end of inner tube of needle 2 is taken to connect the outer tube one end for extracting needle 2,5 free end of inner tube connects piston 6, and outer tube accommodating chamber is provided with inspection
Survey the detection agent of virus.
Specifically, the detection agent for the detection virus being equipped in the outer tube receiving cavity of several extraction needles 2 is different, detection
Agent can be detection tobacco mosaic viruses disease, tobacco cucumber mosaic virus disease, tobacco potato Y virus disease.
Specifically, piston 6 connects traction element 7, and traction element 7 is circle through the blunt end for extracting needle 2, the cross section of blunt end
Shape structure, the cross section of blunt end are greater than the cross section at tip, and the cross section at tip is ring-dot type structure, and piston 6 can be in inner tube 5
Sliding in accommodating chamber, piston 6 are trapezium structure, one end of the big one end connection traction element 7 in 6 cross section of piston, traction element 7
Through extracting, sealing ring is set at 2 blunt end of needle.
Specifically, at least one handle 3 is connected on shell 1, and fixation member is set inside shell 1, is capable of fixing extraction needle
2, keep extraction needle 2 more firm in insertion plant, fixation member is to be flexibly connected with needle 2 is extracted, and can facilitate and replace shell 1
Internal extraction needle 2.
A kind of application method of tobacco virus detection display, includes the following steps: in embodiment
(1) shell 1 is inserted in and is fixed in tobacco stalk；
(2) it extracts inside the tip insertion tobacco stalk crust of needle 2；
(3) tobacco juice enters inner tube 5, is entered in outer tube accommodating chamber by the free end of inner tube 5, the detection with detection virus
Agent mixing, the variation of detection agent is observed through shell 1.
Specifically, 1 free end of shell of two semicircular structures can be socketed, adjust inner ring area and perimeter it is big
Small, the rotation of 3 one end of handle connects the shell 1 of one of semicircular structure, and the free end of handle 3 is inserted into another semicircle
On the shell 1 of shape structure, handle 3 is connected the shell 1 of two semicircular structures.
Specifically, shell 1 has material composition flexible, can produce deformation and bends, can plant around tobacco stalk
The shape of strain carries out fitting winding, and the structure for forming helix is wrapped on the plant of different thicknesses.
Specifically, extracting the sealing film at 2 tip inlets of needle is release membranes, and sealing film can be melted in tobacco stalk juice
In, juice, which flows into, after sealing film contact juice melts extracts 2 inner tube 5 of needle.
Specifically, shell 1 is transparent shell 1, and extraction 2 tube wall of needle is transparent wall, pulls traction element 7, makes tractive unit
Part 7 is detached from inner tube 5, extracts the juice of tobacco stalk, and transparent shell 1 and transparent extraction needle 2 can observe the extraction of inner tube 5
Tobacco juice and the mixed phenomenon of the intracorporal detection agent of outer tube accommodating chamber.
Specifically, piston 6 is detached from 5 free end of inner tube, and the air pressure in 5 accommodating chamber of inner tube is less than the air pressure extracted outside needle 2,
Juice in tobacco stalk sucks inner tube 5, is entered in outer tube receiving cavity by 5 free end of inner tube, and juice and detection liquid are mixed into
Row detection.
Specifically, tobacco juice mixes unconverted for healthy plant with viral diagnosis agent, generates discoloration or precipitating
For plant in spite of illness, different viral diagnosis agent is housed in different extraction needles 2, to tobacco mosaic viruses disease, tobacco cucumber
The viruses such as mosaic virus, tobacco potato Y virus disease are detected.
Specifically, detection display can carry out virosis detection to doubtful viral plant in laboratory, or directly
It will test display to be arranged on tobacco plant body, the variation of detection agent observed in daily field management, extract 2 tip of needle
It, being capable of normal growth and development to the basic fanout free region of plant body.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
Based on the above description of the preferred embodiments of the present invention, through the above description, related personnel completely can be with
Without departing from the scope of the technological thought of the present invention', various changes and amendments are carried out.The technical scope of this invention
It is not limited to the contents of the specification, it is necessary to determine the technical scope according to the scope of the claims.
Claims (10)
1. a kind of tobacco virus detects display, comprising: several extractions of setting in shell and the housing accommodating chamber
Needle, which is characterized in that the housing cross-sectional is cirque structure, and the shell of cirque structure is at least two semicircle
Structure connection, the shell of two semicircular structures are connected by shaft, and sealing film is equipped at the tip inlets for extracting needle,
The internal ring wall of the shell, central point of the tip towards the shell, the extraction needle are run through in the tip for extracting needle
For double-deck pipe body, described inner tube one end for extracting needle connects described outer tube one end for extracting needle, and the connection of said inner tube free end is lived
Plug, the outer tube accommodating chamber are provided with the detection agent of detection virus.
2. a kind of tobacco virus according to claim 1 detects display, it is characterised in that: several extract the outer of needle
The detection agent for the detection virus being equipped in pipe receiving cavity is different.
3. a kind of tobacco virus according to claim 1 detects display, it is characterised in that: the piston connection traction
Component.
4. a kind of tobacco virus according to claim 3 detects display, it is characterised in that: the traction element runs through
The blunt end for extracting needle.
5. a kind of tobacco virus according to claim 1 detects display, it is characterised in that: the piston can be in institute
State sliding in inner tube accommodating chamber.
6. a kind of tobacco virus according to claim 1 detects display, it is characterised in that: be connected on the shell
A few handle.
7. a kind of application method of tobacco virus detection display according to claim 1, which is characterized in that including such as
Lower step:
(1) shell is inserted in and is fixed in tobacco stalk；
(2) inside the tip insertion tobacco stalk crust for extracting needle；
(3) tobacco juice enters said inner tube, is entered in the outer tube accommodating chamber by the free end of said inner tube, with detection virus
Detection agent mixing, through the shell observation detection agent variation.
8. in a kind of application method (1) of tobacco virus detection display according to claim 7, it is characterised in that:
The skin free edge of two semicircular structures can be socketed, and adjust the area of inner ring and the size of perimeter, the rotation of handle one end
The shell of one of semicircular structure is connected, the free end of handle is inserted on the shell of another semicircular structure, hand
Handle is connected the shell of two semicircular structures.
9. in a kind of application method (2) of tobacco virus detection display according to claim 7, it is characterised in that:
The sealing film for extracting needle tip opening is release membranes, and the sealing film can be melted in tobacco stalk juice.
10. in a kind of application method (3) of tobacco virus detection display according to claim 7, it is characterised in that:
The shell is transparent shell, and the extraction needle wall is transparent wall, pulls traction element, is detached from traction element in described
Pipe, extracts the juice of tobacco stalk, transparent shell and transparent extractions needle can observe the tobacco juice of inner tube extraction with outside
The mixed phenomenon of detection agent in pipe receiving cavity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910292423.8A CN110095456B (en) | 2019-04-12 | 2019-04-12 | Tobacco virus detection and development device and use method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910292423.8A CN110095456B (en) | 2019-04-12 | 2019-04-12 | Tobacco virus detection and development device and use method |
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| Publication Number | Publication Date |
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| CN110095456A true CN110095456A (en) | 2019-08-06 |
| CN110095456B CN110095456B (en) | 2021-08-20 |
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| CN201910292423.8A Active CN110095456B (en) | 2019-04-12 | 2019-04-12 | Tobacco virus detection and development device and use method |
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