CN110089733A - A method of black fungus nutrient solution is prepared using food processing residues - Google Patents
A method of black fungus nutrient solution is prepared using food processing residues Download PDFInfo
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- CN110089733A CN110089733A CN201910403264.4A CN201910403264A CN110089733A CN 110089733 A CN110089733 A CN 110089733A CN 201910403264 A CN201910403264 A CN 201910403264A CN 110089733 A CN110089733 A CN 110089733A
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention belongs to Edible Fungi and manufacture fields, and in particular to a method of black fungus nutrient solution is prepared using food processing residues.The following steps are included: S1, black fungus slant strains culture；Black fungus liquid shaking bottle strain is made in S2, Liquid Culture；S3, solid state fermentation obtain black fungus zymophyte pompon；S4, supernatant is taken after adding pure water to boil black fungus bacterial pompon, then citric acid, malic acid, granulated sugar, honey, pure water mixing preparation, with citric acid tune pH value to 3.8-4.5 is added, it bottles, cover while hot, sterilization, being after the assay was approved black fungus nutrient solution.The present invention is using corncob as the main fermentation raw material of fermented black fungus fermentation, fermentation raw material is easy to get, is at low cost, solve the problems, such as that corncob results in waste of resources as the directly burned processing of waste, the black fungus nutrient solution being prepared is rich in amino acid and polysaccharide, and nutritive value is high.
Description
Technical field
The invention belongs to Edible Fungi and processing technique fields, are more particularly to a kind of utilization food processing residues system
The method of standby black fungus nutrient solution.
Background technique
Black fungus is a kind of edible and medicinal fungi, full of nutrition and with good health-care effect, referred to as " black rarity "
" street cleaner of human body ".Black fungus is rich in carbohydrate, protein and cellulose, and fat content is lower, can improve intestines
Stomach, moistening lung gut purge have good effect to weight-reducing and anti-treat constipation etc..The content of mineral substances such as iron, calcium are high in black fungus simultaneously,
Iron content is about 8 times of pork liver, can improve anaemia, makes the ruddy delicacy of skin.In black fungus also containing polysaccharide, adenosine, colloid,
Melanin and other functional ingredient, have the pharmacological actions such as strengthen immunity, reducing blood lipid, antithrombotic, antitumor, anticoagulation, have compared with
High edible and medical value.
Currently, China produces about 1.5 hundred million tons of corn per year, the yield of corresponding corncob is estimated also at 40,000,000 tons or so, so
A large amount of corncob is other than part is used as the raw material of the products such as furfural, xylitol, greatly by as agricultural wastes,
Direct burning processing, causes the significant wastage of resource.
In addition, there are cost of material height, on tunning without effective in the technology in black fungus liquid fermentation field
The problems such as ground extraction and application.Currently, there is not yet using the corncob of food processing residues as black fungus solid state fermentation culture
The report of black fungus nutrient solution is made in object.
Summary of the invention
To solve the above-mentioned problems, the present invention provides the method for preparing black fungus nutrient solution using food processing residues,
Using corncob as the main fermentation raw material of fermented black fungus fermentation, it is direct as food processing waste to solve corncob
The problem of burned processing results in waste of resources, the black fungus nutrient solution being prepared is rich in amino acid and polysaccharide, nutritive value
It is high.
The object of the present invention is to provide a kind of method for preparing black fungus nutrient solution using food processing residues, features
It is: the described method comprises the following steps:
S1, black fungus slant strains culture
Aseptically by black fungus strain, it is connected in PDA culture medium, and is cultivated 29-32 days at 35-38 DEG C, to
Mycelia covers with inclined-plane, obtains widened black fungus slant strains；
S2, Liquid Culture
In the widened black fungus slant strains access level liquid shaking flask that S1 is obtained, every bottle meets strain 1.5cm2,
30-33 DEG C, shake culture 6-8 days under conditions of 100-180rpm/min, black fungus liquid shaking bottle strain is made；
S3, solid state fermentation:
Using maize cob meal as the primary raw material of black fungus solid-state fermentation culture medium, it is inoculated with black fungus liquid shaking bottle strain, is obtained
Obtain black fungus zymophyte pompon；
S4, the black fungus bacterial pompon 20-25% for obtaining S3, add 2-3 times of pure water to boil 10-15 minutes, take supernatant,
Then citric acid 0.06-0.08%, malic acid 0.02-0.04%, granulated sugar 3-5%, honey 0.5-1.5% is added, adds pure water
To 100%, mixing preparation, with citric acid tune pH value to 3.8-4.5, deployed black fungus seasoning liquid is bottled while hot, is covered,
Sterilization is black fungus nutrient solution after the assay was approved.
Preferably, in S2, the level-one shaking flask expands the preparation of the culture medium of culture are as follows: corn juice 50-150mL, grape
Sugared 5-20g, KH2PO4 1-5g、MgSO4·7H2O 1-5g、ZnSO4·7H2O 0.1-0.5g adds water to be settled to 1000mL, and pH is certainly
So.
Preferably, in S3, the fermentation medium used in the solid state fermentation, raw material composition includes: jade by weight
Rice core powder 60-80%, wheat bran 20-40%, the preparation of fermentation medium are as follows: maize cob meal and wheat bran are stirred, added
Water makes the water content of compost reach 65-70%.
Preferably, the maize cob meal is that corncob crushed to 80-120 mesh to obtain.
Preferably, in S3, the inoculation is to connect black fungus liquid shaking bottle strain for the inoculum concentration of 5-10% with volume ratio
Kind, the condition of culture of solid state fermentation are as follows: 24-28 DEG C, cultivate 18-25 days, mycelia can cover with bottle, obtain black fungus zymophyte
Matter.
Preferably, the formula of the PDA culture medium are as follows: peeled potatoes 200g, glucose 20g, agar 20g add water
It is settled to 1L, pH is natural.
Compared with prior art, the method provided by the invention for preparing black fungus nutrient solution using food processing residues,
It has the advantages that
Using corncob as the main fermentation raw material of fermented black fungus fermentation, fermentation raw material is easy to get, is at low cost the present invention,
And solve the problems, such as that corncob results in waste of resources as the directly burned processing of food processing waste, what is be prepared is black
Agaric nutrient solution is rich in amino acid and polysaccharide, and nutritive value is high.
Specific embodiment
The present invention is described in detail combined with specific embodiments below, but should not be construed as limitation of the invention.It is following
The test method of actual conditions is not specified in embodiment, operates usually according to normal condition, due to not being related to inventive point, thus it is not right
Its step is described in detail.
The formula for the PDA culture medium that the present invention uses are as follows: peeled potatoes 200g, glucose 20g, agar 20g add water fixed
Hold to 1L, pH is natural (Suo Laibao).
The method provided by the invention for preparing black fungus nutrient solution using food processing residues, specifically includes following implementation
Example.
Embodiment 1
A method of black fungus nutrient solution being prepared using food processing residues, the described method comprises the following steps:
S1, black fungus slant strains culture
Aseptically by black fungus strain, it is connected in PDA culture medium, and is cultivated 30 days at 36 DEG C, it is long to mycelia
Full inclined-plane, obtains widened black fungus slant strains；
S2, Liquid Culture
In the widened black fungus slant strains access level liquid shaking flask that S1 is obtained, every bottle meets strain 1.5cm2,
32 DEG C, shake culture 7 days under conditions of 120rpm/min, black fungus liquid shaking bottle strain is made；
S3, solid state fermentation:
Using maize cob meal as the primary raw material of black fungus solid-state fermentation culture medium, it is inoculated with black fungus liquid shaking bottle strain, is obtained
Obtain black fungus zymophyte pompon；
S4, the black fungus bacterial pompon 23% for obtaining S3, add 2 times of pure water to boil 13 minutes, take supernatant, be then added
Citric acid 0.07%, malic acid 0.03%, granulated sugar 4%, honey 1% add pure water to 100%, mixing preparation, with citric acid tune
Deployed black fungus seasoning liquid is bottled while hot, covered, sterilized, be after the assay was approved black fungus nutrition to 4.0 by pH value
Liquid.
Wherein, in S2, the level-one shaking flask expands the preparation of the culture medium of culture are as follows: corn juice 100mL, glucose 15g,
KH2PO4 3g、MgSO4·7H2O3g、ZnSO4·7H2O 0.3g, adds water to be settled to 1000mL, and pH is natural.
Wherein, in S3, the fermentation medium used in the solid state fermentation, raw material composition includes: corn by weight
Core powder 70%, wheat bran 30%, the preparation of fermentation medium are as follows: maize cob meal and wheat bran are stirred, water is added, makes to cultivate
The water content of material reaches 68%.
Above-mentioned maize cob meal is to sieve with 100 mesh sieve corncob crushing.
Wherein, in S3, the inoculation is the inoculum concentration inoculation for being 8% with volume ratio by black fungus liquid shaking bottle strain, Gu
The condition of culture of state fermentation are as follows: 26 DEG C, cultivate 20 days, mycelia can cover with bottle, obtain black fungus Zymocyte.
Embodiment 2
A method of black fungus nutrient solution being prepared using food processing residues, the described method comprises the following steps:
S1, black fungus slant strains culture
Aseptically by black fungus strain, it is connected in PDA culture medium, and is cultivated 32 days at 35 DEG C, it is long to mycelia
Full inclined-plane, obtains widened black fungus slant strains；
S2, Liquid Culture
In the widened black fungus slant strains access level liquid shaking flask that S1 is obtained, every bottle meets strain 1.5cm2,
30 DEG C, shake culture 8 days under conditions of 180rpm/min, black fungus liquid shaking bottle strain is made；
S3, solid state fermentation:
Using maize cob meal as the primary raw material of black fungus solid-state fermentation culture medium, it is inoculated with black fungus liquid shaking bottle strain, is obtained
Obtain black fungus zymophyte pompon；
S4, the black fungus bacterial pompon 20% for obtaining S3, add 3 times of pure water to boil 10 minutes, take supernatant, be then added
Citric acid 0.06%, malic acid 0.02%, granulated sugar 3%, honey 0.5% add pure water to 100%, and mixing preparation uses citric acid
It adjusts pH value to 3.8, deployed black fungus seasoning liquid is bottled while hot, cover, is sterilized, is after the assay was approved black fungus battalion
Nutrient solution.
Wherein, in S2, the level-one shaking flask expands the preparation of the culture medium of culture are as follows: corn juice 50mL, glucose 5g,
KH2PO4 1g、MgSO4·7H2O 1g、ZnSO4·7H2O 0.1g, adds water to be settled to 1000mL, and pH is natural.
Wherein, in S3, the fermentation medium used in the solid state fermentation, raw material composition includes: corn by weight
Core powder 60%, wheat bran 40%, the preparation of fermentation medium are as follows: maize cob meal and wheat bran are stirred, water is added, makes to cultivate
The water content of material reaches 65%.
Above-mentioned maize cob meal is that corncob crushed to 80 meshes to obtain.
Wherein, in S3, the inoculation is the inoculum concentration inoculation for being 5% with volume ratio by black fungus liquid shaking bottle strain, Gu
The condition of culture of state fermentation are as follows: 24 DEG C, cultivate 18 days, mycelia can cover with bottle, obtain black fungus Zymocyte.
Embodiment 3
A method of black fungus nutrient solution being prepared using food processing residues, the described method comprises the following steps:
S1, black fungus slant strains culture
Aseptically by black fungus strain, it is connected in PDA culture medium, and is cultivated 29 days at 38 DEG C, it is long to mycelia
Full inclined-plane, obtains widened black fungus slant strains；
S2, Liquid Culture
In the widened black fungus slant strains access level liquid shaking flask that S1 is obtained, every bottle meets strain 1.5cm2,
33 DEG C, shake culture 6 days under conditions of 100rpm/min, black fungus liquid shaking bottle strain is made；
S3, solid state fermentation:
Using maize cob meal as the primary raw material of black fungus solid-state fermentation culture medium, it is inoculated with black fungus liquid shaking bottle strain, is obtained
Obtain black fungus zymophyte pompon；
S4, the black fungus bacterial pompon 20% for obtaining S3, add 2 times of pure water to boil 15 minutes, take supernatant, be then added
Citric acid 0.08%, malic acid 0.04%, granulated sugar 5%, honey 1.5% add pure water to 100%, and mixing preparation uses citric acid
It adjusts pH value to 4.5, deployed black fungus seasoning liquid is bottled while hot, cover, is sterilized, is after the assay was approved black fungus battalion
Nutrient solution.
Wherein, in S2, the level-one shaking flask expands the preparation of the culture medium of culture are as follows: corn juice 150mL, glucose 20g,
KH2PO4 5g、MgSO4·7H2O5g、ZnSO4·7H2O 0.5g, adds water to be settled to 1000mL, and pH is natural.
Wherein, in S3, the fermentation medium used in the solid state fermentation, raw material composition includes: corn by weight
Core powder 80%, wheat bran 20%, the preparation of fermentation medium are as follows: maize cob meal and wheat bran are stirred, water is added, makes to cultivate
The water content of material reaches 70%.
Above-mentioned maize cob meal is that corncob crushed to 120 meshes to obtain.
Wherein, in S3, the inoculation is the inoculum concentration inoculation for being 10% with volume ratio by black fungus liquid shaking bottle strain, Gu
The condition of culture of state fermentation are as follows: 28 DEG C, cultivate 258 days, mycelia can cover with bottle, obtain black fungus Zymocyte.
In order to verify effect of the invention, we are illustrated by taking embodiment 1 as an example, by black fungus nutrition of the invention
Liquid carries out amino acid detection, and the results are shown in Table 1；The black fungus nutrient solution of 1-3 of the embodiment of the present invention is subjected to polysaccharide inspection
It surveys, the results are shown in Table 2.
The amino acid of the black fungus nutrient solution of the invention of table 1 detects table
The black fungus nutrient solution that the embodiment of the present invention 1 obtains, after carrying out amino acid detection, total amino content is 510mg/
100mL, wherein 7 kinds of methionine, isoleucine, leucine, valine, lysine, threonine and phenylalanine must amino
The total 251.15mg/100mL of acid, it is necessary to half of the amino acid content close to total amino acid content.
The polysaccharide of the black fungus nutrient solution of the invention of table 2 detects table
| Project | Embodiment 1 | Embodiment 2 | Embodiment 3 |
| Polysaccharide (mg/100mL) | 132 | 118 | 125 |
The black fungus nutrient solution that 1-3 of the embodiment of the present invention is obtained is rich in polysaccharide, in every 100mL black fungus nutrient solution extremely
Polysaccharide containing 118mg less.
It should be noted that repeat in order to prevent, description of the invention preferred embodiment and effect, although having described
The preferred embodiment of the present invention, once a person skilled in the art knows basic creative concepts, then can be to these
Embodiment makes other change and modification.So the following claims are intended to be interpreted as including preferred embodiment and falls into
All change and modification of the scope of the invention.
Obviously, various changes and modifications can be made to the invention without departing from essence of the invention by those skilled in the art
Mind and range.In this way, if these modifications and changes of the present invention belongs to the range of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to include these modifications and variations.
Claims (6)
1. a kind of method for preparing black fungus nutrient solution using food processing residues, it is characterised in that: the following steps are included:
S1, black fungus slant strains culture
Aseptically by black fungus strain, it is connected in PDA culture medium, and is cultivated 29-32 days at 35-38 DEG C, to mycelia
Inclined-plane is covered with, widened black fungus slant strains are obtained；
S2, Liquid Culture
In the widened black fungus slant strains access level liquid shaking flask that S1 is obtained, every bottle meets strain 1.5cm2, in 30-33
DEG C, shake culture 6-8 days under conditions of 100-180rpm/min, black fungus liquid shaking bottle strain is made；
S3, solid state fermentation:
Using maize cob meal as the raw material of black fungus solid-state fermentation culture medium, it is inoculated with black fungus liquid shaking bottle strain, obtains black fungus
Zymophyte pompon；
S4, by percentage to the quality, the black fungus bacterial pompon 20-25% that S3 is obtained add 2-3 times of pure water to boil 10-15 points
Clock takes supernatant, and citric acid 0.06-0.08%, malic acid 0.02-0.04%, granulated sugar 3-5%, honey 0.5- is then added
1.5%, add pure water to 100%, mixing preparation, with citric acid tune pH value to 3.8-4.5, by deployed black fungus seasoning liquid
It bottles, cover while hot, sterilization, being after the assay was approved black fungus nutrient solution.
2. the method according to claim 1 for preparing black fungus nutrient solution using food processing residues, which is characterized in that
In S2, every liter of level-one shaking flask expands the preparation of the culture medium of culture are as follows: corn juice 50-150mL, glucose 5-20g,
KH2PO4 1-5g、MgSO4·7H2O1-5 g、ZnSO4·7H2O 0.1-0.5g, adds water to be settled to 1000mL.
3. the method according to claim 1 for preparing black fungus nutrient solution using food processing residues, which is characterized in that
In S3, the solid-state fermentation culture medium raw material composition includes: maize cob meal 60-80%, wheat bran 20-40%, fermentation by weight
The preparation of culture medium are as follows: maize cob meal and wheat bran are stirred, water is added, the water content of compost is made to reach 65-70%.
4. the method according to claim 3 for preparing black fungus nutrient solution using food processing residues, which is characterized in that
The maize cob meal is that corncob crushed to 80-120 mesh to obtain.
5. the method according to claim 1 for preparing black fungus nutrient solution using food processing residues, which is characterized in that
In S3, the inoculation is to be inoculated with black fungus liquid shaking bottle strain for the inoculum concentration of 5-10% with volume ratio, the training of solid state fermentation
The condition of supporting are as follows: 24-28 DEG C, cultivate 18-25 days, mycelia can cover with bottle, obtain black fungus Zymocyte.
6. the method according to claim 1 for preparing black fungus nutrient solution using food processing residues, which is characterized in that
The formula of PDA culture medium described in every liter are as follows: peeled potatoes 200g, glucose 20g, agar 20g add water to be settled to 1L.
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