CN110075146A - A kind of canker sore spray and preparation method thereof - Google Patents
A kind of canker sore spray and preparation method thereof Download PDFInfo
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Abstract
The present invention provides a kind of canker sore sprays and preparation method thereof, belong to treatment canker sore technical field, the component including following volumes number: accounts for 75~85 parts of fragrant plant alcohol extracting thing, 5~15 parts of stem cell extract, 3~8 parts of deionized water, 2~6 parts of preservative and vitamin B20.1~0.5 part；The concentration of excretion body protein matter is 25~35mg/mL in the stem cell extract；The pH value of the canker sore spray is 6.6~7.11.Canker sore aerosol therapy effect provided by the invention is good, feels mild comfortable when use, and without scar after healing, the total effective rate to oral cavity ulcers is 97.22%.
Description
Technical field
The present invention relates to treatment canker sore technical field more particularly to a kind of canker sore spray and its preparation sides
Method.
Background technique
Ulcer spot is more common in lingual surface, lip, gum etc..Sharp ache when canker sore is broken out, local cusalgia is obvious, sternly
Severe one also will affect diet, speak, can also concurrent halitosis, chronic pharyngitis etc., if be not treated in time, pole is caused to daily life
It is big inconvenient.There are many its risk factors, such as local trauma, stimulant, bacterial contamination, endocrine imbalance, gloomy, spiritual tight
It opens to wait and can all cause ulcer.
Recurrent oral ulceration is roughly divided into three kinds: small aphtha (minor aphthous ulcers), big aphtha (major
Aphthous ulcers), herpetiform ulceration (andherpetiform ulcers), and it is most commonly seen with small aphthous ulcer, it is multiple
Hair rate is high.Clinically the treatment of small aphthous canker sore can be divided into: (1) supportive treatment of suiting the medicine to the illness；(2) specially treated is treated；(3)
Prophylactic treatment.The Topical treatment regimens of standard carry out the remissions such as pain usually using some drugs, including analgestic, anti-
Microorganism agent, antiphlogistic, steroids, vitamin B2Deng.It clinically there is no radical cure recurrent oral ulceration completely, Er Qieyi at present
As for, bigger and deep canker sore (major aphthous ulcers) healing after can be partially formed
Scar, with the extension of time, the scar formed after this ulcer healing also can gradually soften, absorbs, become smaller, but local mucous membrane
It is difficult to restore as before.
Summary of the invention
Aiming at the problems existing in the prior art, the present invention provides a kind of canker sore spray and preparation method thereof,
Canker sore aerosol therapy effect provided by the invention is good, feels mild comfortable when use, without scar after healing.
The present invention provides a kind of canker sore spray, the component including following volumes number:
Account for 75~85 parts of fragrant plant alcohol extracting thing, 5~15 parts of stem cell extract, 3~8 parts of deionized water, preservative 2~6
Part and vitamin B20.1~0.5 part；
The concentration of excretion body protein matter is 25~35mg/mL in the stem cell extract；
The pH value of the canker sore spray is 6.6~7.11.
Preferably, the component including following volumes number:
Account for 80 parts of fragrant plant alcohol extracting thing, 10 parts of stem cell extract, 5 parts of deionized water, 4 parts of preservative and vitamin B2
0.3 part；
The concentration of excretion body protein matter is 30mg/mL in the stem cell extract.
Preferably, the preparation method for accounting for fragrant plant alcohol extracting thing, comprising the following steps:
1) 1~2h of extraction after fragrant plant mixes with the first ethanol solution will be accounted for, the first filtrate and the first filter residue are obtained after filtering；
The volumn concentration of first ethanol solution is 60~70%；
2) 1~2h is extracted after the first filter residue for obtaining the step 1) is mixed with the second ethanol solution, is obtained after filtering
Second filtrate and the second filter residue；The volumn concentration of second ethanol solution is 55~65%；
3) 1~2h is extracted after the second filter residue for obtaining the step 2) is mixed with third ethanol solution, is obtained after filtering
Third filtrate and third filter residue；The volumn concentration of the third ethanol solution is 55~65%；
4) the second filtrate that the first filtrate that the step 1) obtains, step 2) obtain and the third that step 3) obtains are filtered
Liquid mixing, obtains filtrate, the filtrate decompression is concentrated, obtain accounting for fragrant plant alcohol extracting thing；The body for accounting for fragrant plant alcohol extracting thing
Product is (1~5) with the weight ratio for accounting for fragrant plant: 1.
Preferably, it is 1:(15~20 that the step 1), which accounts for fragrant plant and the mass ratio of the first ethanol solution).
Preferably, the mass ratio of first filter residue of step 2) and the second ethanol solution is 1:(8~12).
Preferably, the mass ratio of second filter residue of step 3) and third ethanol solution is 1:(6~8).
Preferably, the stem cell extract preparation method the following steps are included:
A, using the method for routine culture human umbilical cord mesenchymal stem cells, human umbilical cord mesenchymal stem cells and people's umbilical cord are obtained
Mescenchymal stem cell culture supernatant；The quantity of the human umbilical cord mesenchymal stem cells is 3 × 108It is a；
B, ultrasonication is carried out after mixing the human umbilical cord mesenchymal stem cells with physiological saline, is received using conventional method
Collect excretion body；
C, the excretion body in human umbilical cord mesenchymal stem cells culture supernatant is collected using conventional method；
D, it after mixing the obtained excretion body of excretion body that the step c is obtained, step d and physiological saline, obtains dry thin
Born of the same parents' extract, the concentration of excretion body protein matter is 25~35mg/mL in the stem cell extract.
Preferably, the excretion body that the step c is obtained carries out BCA determination of protein concentration, and the concentration of excretion body protein matter is
9.5mg/ml。
Preferably, the excretion body that the step d is obtained carries out BCA determination of protein concentration, and the concentration of excretion body protein matter is
53.6mg/ml。
The present invention also provides the preparation methods of the canker sore spray described in above-mentioned technical proposal, comprising: will be described
Vitamin B2After aqueous slkali dissolves with account for fragrant plant alcohol extracting thing, after deionized water mixes, then successively mentioned with preservative, stem cell
It takes object to mix, obtains canker sore spray.
The present invention provides a kind of canker sore spray, the component including following volumes number: fragrant plant alcohol extracting thing is accounted for
75~85 parts, 5~15 parts of stem cell extract, 3~8 parts of deionized water, 2~6 parts of preservative and vitamin B20.1~0.5
Part；The concentration of excretion body protein matter is 25~35mg/mL in the stem cell extract；The pH value of the canker sore spray
It is 6.6~7.11.
In the present invention, the fragrant plant alcohol extracting thing that accounts for includes a variety of aromatic alcohol compounds such as aromatic alcohol and geraniol,
There is anti-inflammatory, antipruritic, moisturizing and repair using fragrant plant alcohol extracting thing is accounted for；Excretion body protein matter in stem cell extract has
Effect improves oral cavity partial inflammation, promotes mouth epithelial cells growth to repair scar position；Vitamin B2 is to canker sore
Treatment has the effect of promotion.After curing canker sore using canker sore spray provided by the invention, no scar.
The invention has the beneficial effects that:
Canker sore aerosol therapy effect provided by the invention is good, feels mild comfortable when use, without scar after healing,
Total effective rate to oral cavity ulcers is 97.22%.And raw materials used is food-grade, is free of antibiotic and hormone, and it is right
Oral cavity throat is mildly non-stimulated, has no toxic side effect.
Detailed description of the invention
Fig. 1 is that patient uses the 1st day canker sore healing result of canker sore spray；
Fig. 2 is that patient uses the 4th day canker sore healing result of canker sore spray；
Fig. 3 is that patient uses the 8th day canker sore healing result of canker sore spray；
Fig. 4 is that patient uses the 10th day canker sore healing result of canker sore spray.
Specific embodiment
The present invention provides a kind of canker sore spray, the component including following volumes number: fragrant plant alcohol extracting thing is accounted for
75~85 parts, 5~15 parts of stem cell extract, 3~8 parts of deionized water, 2~6 parts of preservative and vitamin B20.1~0.5
Part；The concentration of excretion body protein matter is 25~35mg/mL in the stem cell extract；The pH value of the canker sore spray
It is 6.6~7.11.
Canker sore spray provided by the invention includes that volume parts are 75~85 parts and account for fragrant plant alcohol extracting thing, preferably
It is 78~82 parts, more preferably 80 parts.
In the present invention, the preparation method for accounting for fragrant plant alcohol extracting thing, preferably includes following steps:
1) 1~2h of extraction after fragrant plant mixes with the first ethanol solution will be accounted for, the first filtrate and the first filter residue are obtained after filtering；
The volumn concentration of first ethanol solution is 60~70%；
2) 1~2h is extracted after the first filter residue for obtaining the step 1) is mixed with the second ethanol solution, is obtained after filtering
Second filtrate and the second filter residue；The volumn concentration of second ethanol solution is 55~65%；
3) 1~2h is extracted after the second filter residue for obtaining the step 2) is mixed with third ethanol solution, is obtained after filtering
Third filtrate and third filter residue；The volumn concentration of the third ethanol solution is 55~65%；
4) the second filtrate that the first filtrate that the step 1) obtains, step 2) obtain and the third that step 3) obtains are filtered
Liquid mixing, obtains filtrate, the filtrate decompression is concentrated, obtain accounting for fragrant plant alcohol extracting thing；The body for accounting for fragrant plant alcohol extracting thing
Product is (1~5) with the weight ratio for accounting for fragrant plant: 1.
The present invention will preferably account for after fragrant plant mixes with the first ethanol solution 1~2h of extraction, obtained after filtering the first filtrate and
First filter residue.In the present invention, the time of the extraction is more preferably 1.5h.In the present invention, it is described account for fragrant plant preferably account for
Fragrant plant fresh flower.In the present invention, the volumn concentration of first ethanol solution is preferably 60~70%, more preferably
65%.In the present invention, the mass ratio for accounting for fragrant plant and the first ethanol solution is preferably 1:(15~20), more preferably 1:
18.In the present invention, the extraction is preferably carried out using the conventional common reflux extraction method of Chinese medicine.
The present invention extracts 1~2h after preferably mixing the first obtained filter residue with the second ethanol solution, and the is obtained after filtering
Two filtrates and the second filter residue.The time of the extraction is more preferably 1.5h in the present invention.In the present invention, second ethyl alcohol
The volumn concentration of solution is preferably 55~65%, and more preferably 60%.In the present invention, first filter residue and the second second
The mass ratio of alcoholic solution is preferably 1:(8~12), more preferably 1:10.
The present invention extracts 1~2h after preferably mixing the second obtained filter residue with third ethanol solution, and the is obtained after filtering
Three filtrates and third filter residue.In the present invention, the time of the extraction is more preferably 1.5h.In the present invention, the third second
The volumn concentration of alcoholic solution is preferably 55~65%, and more preferably 60%.In the present invention, second filter residue and third
The mass ratio of ethanol solution is preferably 1:(6~8).
In the present invention, described to account in fragrant plant alcohol extracting thing containing there are many aromatic alcohol compound (the total ingredients of Zhan
24.92%) such as aromatic alcohol (accounting for about 5.97%) and geraniol (accounting for about 10.2%).In the present invention, described to account for fragrant plant alcohol extracting
Object has the function of anti-inflammatory, antipruritic, moisturizing and reparation.
It is 5~15 parts of stem cell extract that canker sore spray provided by the invention, which includes volume fraction, preferably 8
~12 parts, more preferably 10 parts.
In the present invention, the preparation method of the stem cell extract preferably includes following steps:
A, using the method for routine culture human umbilical cord mesenchymal stem cells, human umbilical cord mesenchymal stem cells and people's umbilical cord are obtained
Mescenchymal stem cell culture supernatant；The quantity of the human umbilical cord mesenchymal stem cells is 3 × 108It is a；
B, ultrasonication is carried out after mixing the human umbilical cord mesenchymal stem cells with physiological saline, is received using conventional method
Collect excretion body；
C, the excretion body in human umbilical cord mesenchymal stem cells culture supernatant is collected using conventional method；
D, it after mixing the obtained excretion body of excretion body that the step c is obtained, step d and physiological saline, obtains dry thin
Born of the same parents' extract, the concentration of excretion body protein matter is 25~35mg/mL in the stem cell extract.
Present invention preferably employs the methods of routine culture human umbilical cord mesenchymal stem cells, obtain human umbilical cord mesenchymal stem cells
With human umbilical cord mesenchymal stem cells culture supernatant.The present invention does not have special limit to the source of the human umbilical cord mesenchymal stem cells
It is fixed, using routine.In the present invention, the quantity for cultivating obtained human umbilical cord mesenchymal stem cells is preferably 3 × 108
It is a.
The present invention carries out ultrasonication after preferably mixing the human umbilical cord mesenchymal stem cells with physiological saline, using normal
Rule method collects excretion body.In the present invention, the quantity of the human umbilical cord mesenchymal stem cells and the volume ratio of physiological saline are excellent
It is selected as 3 × 108It is a: 300mL.
In the present invention, the parameter setting of the ultrasonication is preferably 600W；4℃；Work 2s interval 2s ultrasound
10min。
Obtained ultrasonication object is preferably centrifuged 10min by the present invention at 300g, 4 DEG C, removes cell fragment, in collection
Clearly, then 2500g, 4 DEG C of centrifugation 30min collect supernatant to remove Apoptosis corpusculum, and 13000g is centrifuged 1h and removes blood platelet
Impurity collects supernatant, and last 100000g, 4 DEG C of centrifugation 70min obtain excretion body.Obtained excretion body is carried out BCA by the present invention
Determination of protein concentration, the concentration of excretion body protein matter are 9.5mg/ml.
Present invention preferably employs conventional methods to collect the excretion body in human umbilical cord mesenchymal stem cells culture supernatant.This hair
It is bright that human umbilical cord mesenchymal stem cells culture supernatant is preferably centrifuged 10min at 2000g, 4 DEG C, cell fragment is removed, is collected
Supernatant, 13000g are centrifuged 1h and remove blood platelet impurity, collect supernatant, last 100000g, 4 DEG C of centrifugation 70min obtain excretion body.
Obtained excretion body is carried out BCA determination of protein concentration by the present invention, and the concentration of excretion body protein matter is 53.6mg/ml.
On the excretion body and human umbilical cord mesenchymal stem cells culture that the present invention obtains broken human umbilical cord mesenchymal stem cells
After the excretion body that clear liquid obtains is dissolved in after physiological saline mixing in equal volume respectively, stem cell extract is obtained.
In the present invention, the concentration of excretion body protein matter is 25~35mg/mL in the stem cell extract, preferably
30mg/mL.In the present invention, the stem cell extract is effectively improved oral cavity partial inflammation, promotes mouth epithelial cells growth
To repair scar position.
It is 3~8 parts of deionized water that canker sore spray provided by the invention, which includes volume parts, preferably 5 parts.
It is 2~6 parts of preservative that canker sore spray provided by the invention, which includes volume parts, preferably 4 parts.
In the present invention, the preparation method of the preservative preferably includes: by methyl p-hydroxybenzoate and 1,3- fourth two
Alcoholic solution mixing stirs to clarify, and sufficiently dissolves, obtains preservative.In the present invention, the matter of the methyl p-hydroxybenzoate
Amount and the volume ratio of 1,3 butylene glycol solution are preferably 15g:250ml.The present invention does not have the concentration of the 1,3 butylene glycol solution
There is particular determination, using the concentration of conventional dissolution methyl p-hydroxybenzoate.
Oral spray provided by the invention includes the vitamin B that volume parts are 0.1~0.5 part2, preferably 0.3 part.
In the present invention, the vitamin B2The effect for having promotion to the treatment of canker sore.
In the present invention, the pH value of the oral spray is 6.6~7.11.In the present invention, canker sore spray is adjusted
The reagent that mist agent uses is preferably sodium hydroxide solution and citric acid.In the present invention, the concentration of the sodium hydroxide is preferably
1mol/L。
The present invention also provides the preparation methods of the canker sore spray described in above-mentioned technical proposal, comprising: will be described
Vitamin B2After aqueous slkali dissolves with account for fragrant plant alcohol extracting thing, after deionized water mixes, then successively mentioned with preservative, stem cell
It takes object to mix, obtains canker sore spray.
In the present invention, the aqueous slkali is preferably sodium hydroxide solution.In the present invention, the concentration of the sodium hydroxide
Preferably 1mol/L.
In the present invention, the canker sore spray is slightly in faint yellow, transparent clarification, mildly bitter flavor.
In the present invention, the application method of the canker sore spray preferably includes: it is early, middle and late and each 1 time before sleeping,
5ml/ times, totally 4 times, it must not drink or feed in 30 minutes after administration every time.
Technical solution provided by the invention is further described in detail combined with specific embodiments below, skill of the invention
Art scheme includes but is not limited to following embodiment.
Embodiment 1
The preparation method of canker sore spray:
Step 1: the preparation of preservative: 250ml 1,3-BDO is added in precise 15g methyl p-hydroxybenzoate
Solution stirs to clarify, and sufficiently dissolves, and stands half an hour observation whether there is or not insoluble matter precipitation, obtains preservative；
Step 2: amount of liquid precise vitamin B is prepared by practical20.06g is added a little sodium hydroxide solution, fills
After dividing dissolution, accurate measure accounts for fragrant plant alcohol extracting thing 16ml, and deionized water 1ml is added；
Step 3: taking preservative 0.08ml, is mixed into above-mentioned solution, stirring, so that being uniformly mixed；
Step 4: taking stem cell extract 2ml, stirring, so that being uniformly mixed, finally uses sodium hydroxide or sodium citrate tune
Saving pH value is 7.0 to get canker sore spray 20ml.
Account for fragrant plant alcohol extracting thing accounts for fragrant plant fresh flower the preparation method comprises the following steps: taking, refluxing extraction the 3 times: 1st time plus 18 times amount
65% ethyl alcohol extracts 1.5h；2 times plus 10 times of 60% ethyl alcohol extract 1.5h；3rd time plus 8 times of 60% ethyl alcohol extract 1.5h.Every time
Three layers of filtered through gauze, merging filtrate concentrate filtrate to and account for 5 times of volumes of fragrant plant fresh flower weight, must account for fragrant plant alcohol extracting thing；
Account in fragrant plant alcohol extracting thing containing there are many aromatic alcohol compound (the total ingredient 24.92% of Zhan) such as aromatic alcohol (accounting for about 5.97%) and
Geraniol (accounts for about 10.2%).
Stem cell extract the preparation method comprises the following steps: filling stem cell in vitro pilot scale culture between people's umbilical cord: choose people's umbilical cord between
Stem cell seed cell is filled, by 1 × 105A cell density is seeded to (GMP grades) of four T175 culture bottles cultures, according to conventional thin
Born of the same parents cultivate operation and cell are collected after centrifugation after digestion when cell melts rate up to 95% or more, then press 4 × 107A cell density passes
In generation, is seeded to cell factory (GMP grades), and addition is cultivated without phenol red complete medium, and a full dose is carried out in incubation and is changed
Liquid can harvest to obtain 3 × 10 when until cell confluency is up to 95% or more8Umbilical cord mesenchymal stem cells, and collector's navel
Band mescenchymal stem cell culture supernatant；
The human umbilical cord mesenchymal stem cells of collection carry out ultrasonic disruption: pressing 1 × 108Cell quantity is added to 100ml physiology
Salt water (0.9% sodium-chloride water solution), cell crushing instrument parameter is set as (600W；4℃；Work 2s interval 2s ultrasound
10min), 300g, 4 DEG C of centrifugation 10min remove cell fragment, collect supernatant, and then 2500g, 4 DEG C of centrifugation 30min are thin to remove
Born of the same parents' apoptotic body collects supernatant, and 13000g is centrifuged 1h and removes blood platelet impurity, collects supernatant, last 100000g, 4 DEG C of centrifugations
70min obtains excretion body precipitating, carries out BCA determination of protein concentration, and the concentration of excretion body protein matter is 9.5mg/ml；
Mescenchymal stem cell culture supernatant is collected, 2000g, 4 DEG C of centrifugation 10min remove cell fragment, collect supernatant,
13000g is centrifuged 1h and removes blood platelet impurity, collects supernatant, and last 100000g, 4 DEG C of centrifugation 70min obtain excretion body precipitating,
BCA determination of protein concentration is carried out, the concentration of excretion body protein matter is 53.6mg/ml；
It is pressed with stem cell culture supernatant excretion body according to surveyed protein concentration after mescenchymal stem cell excretion body after broken
Ratio mixing, which is added to together again in physiological saline, obtains required whole protein concentration (30mg/ml), is stirred until homogeneous up to stem cell
Extract.
Embodiment 2
The preparation method of canker sore spray:
Step 1: the preparation of preservative: 250ml 1,3-BDO is added in precise 15g methyl p-hydroxybenzoate
Solution stirs to clarify, and sufficiently dissolves, and stands half an hour observation whether there is or not insoluble matter precipitation, obtains preservative；
Step 2: amount of liquid precise vitamin B is prepared by practical20.02g is added a little sodium hydroxide solution, fills
After dividing dissolution, prepared by correct amount Example 1 accounts for fragrant plant alcohol extracting thing 14ml, and deionized water 4ml is added；
Step 3: taking preservative 0.08ml, is mixed into above-mentioned solution, stirring, so that being uniformly mixed；
Step 4: stem cell extract 1ml prepared by Example 1, stirring finally use hydroxide so that being uniformly mixed
It is 7.0 to get canker sore spray 20ml that sodium or sodium citrate, which adjust pH value,.
Embodiment 3
The preparation method of canker sore spray:
Step 1: the preparation of preservative: 250ml 1,3-BDO is added in precise 15g methyl p-hydroxybenzoate
Solution stirs to clarify, and sufficiently dissolves, and stands half an hour observation whether there is or not insoluble matter precipitation, obtains preservative；
Step 2: amount of liquid precise vitamin B is prepared by practical20.1g is added a little sodium hydroxide solution, fills
After dividing dissolution, prepared by correct amount Example 1 accounts for fragrant plant alcohol extracting thing 15ml, and deionized water 1.86ml is added；
Step 3: taking preservative 0.04ml, is mixed into above-mentioned solution, stirring, so that being uniformly mixed；
Step 4: stem cell extract 3ml prepared by Example 1, stirring finally use hydroxide so that being uniformly mixed
It is 7.0 to get canker sore spray 20ml that sodium or sodium citrate, which adjust pH value,.
The physical and chemical index of canker sore spray prepared by embodiment 1 is as follows:
Character: this product is quantitative pressing type aerosol agent, and liquid is slightly in faint yellow, transparent clarification, the oral cavity of mildly bitter flavor in bottle
Ulcer spray.
Quality testing: (1) dipping a small amount of mouthspray liquid with pH test paper, and measuring pH value is 6-7.(2) liquid is distinguished
It is packaged in transparent spray bottle, observes in 4 DEG C of refrigerator cold-storages and in placing one week or so in 37 DEG C of insulating box without lamination.
3 months are stood, also without lamination, feels unchanged after, smell is unchanged, no denaturalization phenomenon.
Irritation detection: zoopery is taken, takes cavy 32, is randomly divided into 4 groups, multiple dosing is the same as single-dose mode
In same position successive administration, administration time is identical with metering, and 4d is used continuously.Skin test appraisal result shows single and more
After secondary use spray and ethanol solution, no erythema and oedema (average response value < 0.5) show canker sore spray more
It is mild non-stimulated.Standards of grading are ground referring to " Chemical induced irritation, anaphylaxis and hemolytic investigative technique guideline " project
Study carefully a group Chemical induced irritation, anaphylaxis and hemolytic investigative technique guideline State Food and Drug Administration,
2005:2-7.The results are shown in Table 1.
1 mouthspray liquid of table is to skin irritation mean scores
Embodiment 4
Dental ulcer treatment effect test
General data:
With in January, 2018~2018 year December the canker sore aerosol therapy prepared using embodiment 1 72 mouths
Chamber ulcers are research object, wherein male 41, female 31, the age 9~71 years old, average (41.6 ± 3.0) year.It is included in mark
Quasi-: 1. clinical diagnosis is diagnosed as canker sore；2. patient agrees to this research and signature informed consent form.Exclusion criteria: it excludes
Oral cavity malignant disease, disease of immune system patient.Two groups of patient's general information are comparable (P > 0.05).
Method:
Patient's Dobell's solution is given before tested to carry out blotting surface with disinfecting cotton swab containing gargling.Patient then uses reality
Apply the canker sore spray of the preparation of example 1, it is early, middle and late and before sleeping it is each primary, 5ml/ times, totally 4 times.It is right after all patient medications
Patient carries out observation in 30 minutes, sees whether the sign of any possible acute allergic reaction, and every time 30 after administration
It must not drink or feed in minute.
Observation evaluation curative effect index:
Ulcer spot size, exudate level (being shown in Table 2) are carried out the 1st, 4,8 and 10 day for receiving mouthspray treatment
Measurement evaluation.It obtains in patient group and pretherapy and post-treatment pain scores between group, patient pain's degree is carried out using VAS pain scores
Scoring, total score are 10 points, and 0 point of expression is painless, and score is higher, and expression pain is more serious.
The classification of 2 exudate level of table
| Classification | Exudate level (Exudation Level) |
| 0 | Nothing |
| 1 | A small amount of exudate |
| 2 | Medium exudate |
| 3 | A large amount of exudates, with pseudomembrane |
Finally, using patient's curative effect between opinion poll point system comparative group.Effective: pain and ulcer disappear in patient 3d；
Effective: patient pain mitigates, healing in 4~7d of ulcer；Invalid: patient pain aggravates, and ulcer does not heal.This treatment data is adopted
Collection is handled in strict accordance with principle of statistics and with software SPSS17.0 analysis, and P < 0.05 indicates that difference has statistical significance.
The results show that being restored such as using being almost recovered in after oral spray 4 days to the 10th day observation ulcer spot scar
Just, oral mucosa repairing effect is good.After treatment, patient's total effective rate 97.22%, data difference has statistical significance between group
(P<0.05).It the results are shown in Table 3~6.
3 two groups of patient outcomes of table [n (%)]
4 ulcer size of table reduces situation
| Time | Group | Ulcer size (mm) | P value |
| 1st day | Experimental group | 5.20±0.20 | 0.207 |
| 4th day | Experimental group | 2.70±2.40 | 0.012 |
| 8th day | Experimental group | 0.20±0.71 | 0.000 |
| 10th day | Experimental group | 0.00±0.00 | 0.000 |
5 exudate level of table compares
| Time | Group | Exudate level | P value |
| 1st day | Experimental group | 1.40±0.10 | 0.441 |
| 4th day | Experimental group | 0.60±0.20 | 0.030 |
| 8th day | Experimental group | 0.00±0.00 | 0.000 |
| 10th day | Experimental group | 0.00±0.00 | 0.000 |
6 canker sores VAS of table scoring is compared
| Time | Group | VAS scoring | P value |
| 1st day | Experimental group | 7.00±0.40 | 0.081 |
| 4th day | Experimental group | 2.50±1.36 | 0.010 |
| 8th day | Experimental group | 0.00±0.71 | 0.000 |
| 10th day | Experimental group | 0.00±0.00 | 0.000 |
Comparative example 1
General data:
With in January, 2018~2018 year December the 72 canker sore patients treated using this mouthspray be research pair
As, wherein male 41, female 31, the age 9~71 years old, average (41.6 ± 3.0) year.Be included in standard: 1. clinical diagnosis is diagnosed as
Recurrent oral ulceration；2. canker sore diameter less than 8cm, 1~3 not etc., the duration be less than 72h.Exclusion criteria: it excludes
Oral cavity malignant disease, disease of immune system patient.
Control group patient is sprayed as placebo using physiological saline, it is early, middle and late and before sleeping it is each once, 5ml/ times,
Totally 4 times.Remaining is the same as embodiment 3.
After treatment, the total effective rate of control group is 66.67%, and data difference has statistical significance (P < 0.05) between group,
It the results are shown in Table 7~10.
7 two groups of patient outcomes of table [n (%)]
| Group | Significantly | Effectively | In vain | Total effective rate |
| Control group | 20(55.56) | 4(11.11) | 12(33.33) | 24(66.67) |
| χ2It examines | 2.130 | 2.144 | 8.881 | 9.116 |
| P value | 0.152 | 0.108 | 0.014 | 0.003 |
8 ulcer size of table reduces situation
| Time | Group | Ulcer size (mm) | P value |
| 1st day | Control group | 4.80±1.44 | 0.207 |
| 4th day | Control group | 3.70±0.80 | 0.012 |
| 8th day | Control group | 2.30±1.45 | 0.000 |
| 10th day | Control group | 1.80±1.80 | 0.000 |
9 exudate level of table compares
| Time | Group | Exudate level | P value |
| 1st day | Control group | 1.80±0.30 | 0.441 |
| 4th day | Control group | 1.50±0.70 | 0.030 |
| 8th day | Control group | 0.60±0.30 | 0.000 |
| 10th day | Control group | 0.50±0.22 | 0.000 |
10 canker sores VAS of table scoring is compared
| Time | Group | VAS scoring | P value |
| 1st day | Control group | 6.70±0.70 | 0.081 |
| 4th day | Control group | 4.10±1.35 | 0.010 |
| 8th day | Control group | 2.30±0.78 | 0.000 |
| 10th day | Control group | 0.80±0.80 | 0.000 |
By above embodiments, it can be concluded that, canker sore aerosol therapy effect provided by the invention is good, and when use is felt
Mild comfortable, without scar after healing, the total effective rate to oral cavity ulcers is 97.22%.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of canker sore spray, which is characterized in that the component including following volumes number:
Account for 75~85 parts of fragrant plant alcohol extracting thing, 5~15 parts of stem cell extract, 3~8 parts of deionized water, 2~6 parts of preservative and
Vitamin B20.1~0.5 part；
The concentration of excretion body protein matter is 25~35mg/mL in the stem cell extract；
The pH value of the canker sore spray is 6.6~7.11.
2. canker sore spray according to claim 1, which is characterized in that the component including following volumes number:
Account for 80 parts of fragrant plant alcohol extracting thing, 10 parts of stem cell extract, 5 parts of deionized water, 4 parts of preservative and vitamin B20.3 part；
The concentration of excretion body protein matter is 30mg/mL in the stem cell extract.
3. canker sore spray according to claim 1 or 2, which is characterized in that the system for accounting for fragrant plant alcohol extracting thing
Preparation Method, comprising the following steps:
1) 1~2h of extraction after fragrant plant mixes with the first ethanol solution will be accounted for, the first filtrate and the first filter residue are obtained after filtering；It is described
The volumn concentration of first ethanol solution is 60~70%；
2) 1~2h is extracted after the first filter residue for obtaining the step 1) is mixed with the second ethanol solution, obtains second after filtering
Filtrate and the second filter residue；The volumn concentration of second ethanol solution is 55~65%；
3) 1~2h is extracted after the second filter residue for obtaining the step 2) is mixed with third ethanol solution, obtains third after filtering
Filtrate and third filter residue；The volumn concentration of the third ethanol solution is 55~65%；
4) the second filtrate that the first filtrate that the step 1) obtains, step 2) obtain and the third filtrate that step 3) obtains is mixed
It closes, obtains filtrate, the filtrate decompression is concentrated, obtain accounting for fragrant plant alcohol extracting thing；The volume for accounting for fragrant plant alcohol extracting thing with
The weight ratio for accounting for fragrant plant is (1~5): 1.
4. canker sore spray according to claim 3, which is characterized in that the step 1) accounts for fragrant plant and the first ethyl alcohol
The mass ratio of solution is 1:(15~20).
5. canker sore spray according to claim 3, which is characterized in that first filter residue of step 2) and the second second
The mass ratio of alcoholic solution is 1:(8~12).
6. canker sore spray according to claim 3, which is characterized in that second filter residue of step 3) and third second
The mass ratio of alcoholic solution is 1:(6~8).
7. canker sore spray according to claim 1 or 2, which is characterized in that the preparation of the stem cell extract
Method the following steps are included:
A, it using the method for routine culture human umbilical cord mesenchymal stem cells, obtains filling between human umbilical cord mesenchymal stem cells and people's umbilical cord
Matter stem cell culture supernatant；The quantity of the human umbilical cord mesenchymal stem cells is 3 × 108It is a；
B, ultrasonication is carried out after mixing the human umbilical cord mesenchymal stem cells with physiological saline, is collected using conventional method outer
Secrete body；
C, the excretion body in human umbilical cord mesenchymal stem cells culture supernatant is collected using conventional method；
D, it after mixing the obtained excretion body of excretion body that the step c is obtained, step d and physiological saline, obtains stem cell and mentions
Object is taken, the concentration of excretion body protein matter is 25~35mg/mL in the stem cell extract.
8. canker sore spray according to claim 7, which is characterized in that the excretion body that the step c is obtained carries out
BCA determination of protein concentration, the concentration of excretion body protein matter are 9.5mg/ml.
9. canker sore spray according to claim 7, which is characterized in that the excretion body that the step d is obtained carries out
BCA determination of protein concentration, the concentration of excretion body protein matter are 53.6mg/ml.
10. the preparation method of canker sore spray according to any one of claims 1 to 9, comprising: by the vitamin B2Through
After aqueous slkali dissolution with account for fragrant plant alcohol extracting thing, after deionized water mixes, then successively mixed with preservative, stem cell extract,
Obtain canker sore spray.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110652491A (en) * | 2019-09-30 | 2020-01-07 | 广州赛琅生物技术有限公司 | Skin care product with effect of removing striae gravidarum and preparation method and application thereof |
| CN110721198A (en) * | 2019-11-08 | 2020-01-24 | 赵凯 | Preparation process of exosome spray for preventing epilepsy from producing brain injury |
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| CN109010523A (en) * | 2018-05-30 | 2018-12-18 | 北京壹典壹生生物技术有限公司 | A kind of canker sore ointment machin preparation method prepared with umbilical cord mesenchymal stem cells secrete cytokines |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110652491A (en) * | 2019-09-30 | 2020-01-07 | 广州赛琅生物技术有限公司 | Skin care product with effect of removing striae gravidarum and preparation method and application thereof |
| CN110652491B (en) * | 2019-09-30 | 2021-09-21 | 广州赛琅生物技术有限公司 | Skin care product with effect of removing striae gravidarum and preparation method and application thereof |
| CN110721198A (en) * | 2019-11-08 | 2020-01-24 | 赵凯 | Preparation process of exosome spray for preventing epilepsy from producing brain injury |
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