CN100374122C - Calf serum deprotein oral cavity ointment and its preparation method - Google Patents
Calf serum deprotein oral cavity ointment and its preparation method Download PDFInfo
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Abstract
The present invention discloses a deproteinized calf serum oral cavity ointment and a preparing method thereof. The oral cavity ointment comprises the compositions by weight of 0.1 to 0.4 share of dry deproteinized calf serum extract powder, 4 to 6 shares of benzocaine, 12.8 to 14.4 shares of sodium carboxymethyl cellulose, 16 to 24 shares of pectin, 004 to 0.06 share of ethylparaben, 0.8 to 1.2 shares of menthol, 20 to 30 shares of liquid paraffin and 30 to 40 shares of white vaseline. The preparing method comprises the steps that dry deproteinized calf serum extract powder is prepared; 4 to 6 shares by weight of benzocaine, 12.8 to 14.4 shares by weight of sodium carboxymethyl cellulose, 16 to 24 shares by weight of pectin, 004 to 0.06 share by weight of ethylparaben, 0.8 to 1.2 shares by weight of menthol, 20 to 30 shares by weight of liquid paraffin and 30 to 40 shares by weight of white vaseline are taken, uniformly mixed with additives and then sterilized; 0.1 to 0.4 share of the dry deproteinized calf serum extract powder is taken and is uniformly mixed with the sterilized mixture to obtain the deproteinized calf serum oral cavity ointment.
Description
Technical field
The present invention relates to biochemical drug oral cavity ointment preparation and preparation method thereof.
Background technology
At present, the pharmaceutical dosage form of calf serum protein-removing extract has liquid drugs injection, gel and capsule, wherein being most widely used with liquid drugs injection.Liquid drugs injection and capsule are the whole body administrations, can not topical application; Gel is the topical application dosage form, but existing gel poor adhesion, and substrate is water-soluble substances, if be used for partial smearing in the oral cavity, retention time is very short, does not have due effect.
Summary of the invention
The purpose of this invention is to provide a kind of is the oral cavity ointment of active component with the calf serum protein-removing extract.
Calf serum deprotein oral cavity ointment provided by the present invention, its prescription comprises following components by weight portion: calf serum protein-removing extract dry powder 0.1-0.4 part (being equivalent to Deporteinnized calf serum extracting solution 2.5%-10%), benzocaine 4-6 part, sodium carboxymethyl cellulose 12.8-14.4 part, pectin 16-24 part, ethyl hydroxybenzoate 0.04-0.06 part, Mentholum 0.8-1.2 part, liquid paraffin 20-30 part, white vaseline 30-40 part.
The prescription of preferred calf serum deprotein oral cavity ointment is: 0.2 part in calf serum protein-removing extract dry powder, 5 parts of benzocaine, 13.6 parts of sodium carboxymethyl cellulose, 20 parts of pectin, 0.05 part of ethyl hydroxybenzoate, 1 part of Mentholum, 25 parts of liquid paraffin, 35.15 parts of white vaseline.
Second purpose of the present invention provides a kind of preparation method of above-mentioned calf serum deprotein oral cavity ointment.
The preparation method of calf serum deprotein oral cavity ointment provided by the present invention may further comprise the steps:
1) preparation calf serum protein-removing extract dry powder;
2) take off and state components by weight portion: benzocaine 4-6 part, sodium carboxymethyl cellulose 12.8-14.4 part, pectin 16-24 part, ethyl hydroxybenzoate 0.04-0.06 part, Mentholum 0.8-1.2 part, liquid paraffin 20-30 part, white vaseline 30-40 part; Behind above-mentioned substrate and additive mix homogeneously, sterilization;
3) get calf serum protein-removing extract dry powder 0.1-0.4 part that step 1) prepares, with step 2) behind the mixture mixing of sterilization, obtain calf serum deprotein oral cavity ointment.
In above-mentioned preparation method, the preparation method of calf serum protein-removing extract dry powder can be with reference to patent (a kind of method of extracting calf blood protein-removed extraction of the previous application of the inventor in the described step 1), the patent No.: ZL 96120777.9) promptly adopts 1-6 monthly age children cattle venous blood, separation of serum, use the ethanol Deproteinization, ethanol is removed in decompression, in the liquid of remainder, add the compound protein enzyme hydrolysis, centrifugal, stay supernatant, supernatant is carried out ultrafiltration hold back, the filtrate that collection obtains is calf blood (protein removed) extracting solution (the solid quality percentage composition is about 4%), after the degerming, lyophilizing obtains calf blood protein-removed extraction dry powder; Described young cattle breeds is preferably the purebred cattle in northeast; During with the ethanol Deproteinization, used ethanol is the ethanol of quality percentage composition 96%, and the ethanol consumption is the twice of serum volume; Alcoholic acid method is removed in decompression: use the rotary evaporation in vacuo instrument, 37 ℃, 1 atmospheric pressure decompression removes ethanol; Time with the compound protein enzyme hydrolysis is 20-28 hour, is preferably 24 hours; Adding the centrifugal condition of the laggard row of compound protein enzyme hydrolysis is: 8000-12000rpm, 4 ℃, 20-40 minute, be preferably 10000rpm, 4 ℃, 30 minutes; It is 4000-6000 dalton that supernatant is carried out the aperture that ultrafiltration cuts used hollow fiber column, is preferably 5000 dalton.
Step 2) addition of various substrate and additive (ratio of weight and number) is preferably in: 5 parts of benzocaine, 13.6 parts of sodium carboxymethyl cellulose, 20 parts of pectin, 0.05 part of ethyl hydroxybenzoate, 1 part of Mentholum, 25 parts of liquid paraffin, 35.15 parts of white vaseline; The condition that substrate and additive agent mixture are sterilized is: under 121 ℃, and autoclaving 20-30min.
The invention provides a kind of is the oral cavity ointment and preparation method thereof of active component with the calf serum protein-removing extract.This oral cavity ointment can be used for the treatment of various oral diseases, as the pain of oral mucosa, gingiva, lip and damage etc., specifically comprise recurrent stomatitis, herpetic stomatitis, radiation stomatitis, chronic angular cheilitis, gingivitis, periodontitis, take off after pressing pain, teething pain, tartar curettage and gingivectomy, the edge of the covering of decorative sursery surgical wound surface and denture is joined etc. in the mucosa transplantation postoperative wound covering, extraction, oral cavity.Relieving pain of the present invention, oral cavity partial are used, pain relieving at once, and viscosity is moderate, and the oral cavity retention time is suitable, has remedied the defective that existing injection and capsule can not oral cavity partial be used, and has also overcome the deficiency of gel adhesion difference.In addition, this oral cavity ointment preparation method is simple, has the feasibility of suitability for industrialized production.The present invention has higher actual application value in stomatology and field of medicaments.
The invention will be further described below in conjunction with specific embodiment.
The specific embodiment
Method therefor is conventional method if no special instructions among the following embodiment.Described percentage composition is the quality percentage composition.
The preparation of embodiment 1, calf serum deprotein oral cavity ointment
Produce 1000 calf serum deprotein oral cavity ointments (calf serum protein-removing extract dry powder content: 0.1%, be equivalent to contain Deporteinnized calf serum extracting solution 2.5%), detailed process may further comprise the steps:
1, the preparation of calf serum protein-removing extract dry powder
Preparation calf serum protein-removing extract dry powder under aseptic condition, concrete grammar is: adopt the venous blood of the purebred cattle in northeast at 1-6 monthly age, 4000rpm, 4 ℃ of centrifugal 15 minutes separation of serum; Get 5L serum, add 10L 96% ethanol, constantly stir, left standstill 60 minutes, make protein denaturation precipitation, through 20000rpm, 4 ℃ of centrifugal 30min, abandon precipitation then, obtain the 12.5L supernatant, utilize the rotary evaporation in vacuo instrument, 37 ℃, 1 atmospheric pressure decompression removes ethanol; Add the 1.5g compound protease in remaining liquid, 37 ℃ of hydrolysis 24 hours 10000rpm, 4 ℃, centrifugal 30 minutes, are stayed supernatant; Supernatant is held back with 5000 dalton's hollow fiber column ultrafilter, collects the filtrate that obtains and is the Deporteinnized calf serum extracting solution, obtains 1000mL (g) Deporteinnized calf serum extracting solution; After the degerming of extracting solution usefulness microporous filter membrane, lyophilizing 24 hours promptly gets 40g calf serum protein-removing extract dry powder.
2, the mixing of substrate and additive and sterilization
Get benzocaine 250g, sodium carboxymethyl cellulose 720g, pectin 900g, ethyl hydroxybenzoate 3g, Mentholum 60g, liquid paraffin 1150g, white vaseline 1912g, behind above-mentioned substrate and additive mix homogeneously, under 121 ℃, autoclaving 25min.
3, the acquisition of calf serum deprotein oral cavity ointment and packing, detection
Get the calf serum protein-removing extract dry powder 5g of step 1 preparation, behind the mixture mixing of step 2, obtain calf serum deprotein oral cavity ointment through sterilizing.With the packing of ointment tucker, be distributed into per 5,10g/ and prop up two kinds of specifications.The calf serum deprotein oral cavity ointment of present embodiment 1 preparation is qualified after testing.
The preparation of embodiment 2, calf serum deprotein oral cavity ointment
Produce 1000 calf serum deprotein oral cavity ointments (calf serum protein-removing extract dry powder content: 0.2%, be equivalent to contain Deporteinnized calf serum extracting solution 5%), detailed process may further comprise the steps:
1, the preparation of calf serum protein-removing extract dry powder
Preparation calf serum protein-removing extract dry powder under aseptic condition, concrete grammar is: adopt the venous blood of the purebred cattle in northeast at 1-6 monthly age, 4000rpm, 4 ℃ of centrifugal 15 minutes separation of serum; Get 5L serum, add 10L 96% ethanol, constantly stir, left standstill 60 minutes, make protein denaturation precipitation, through 20000rpm, 4 ℃ of centrifugal 30min, abandon precipitation then, obtain the 12.5L supernatant, utilize the rotary evaporation in vacuo instrument, 37 ℃, 1 atmospheric pressure decompression removes ethanol; In remaining liquid, add the 1.5g compound protease, 37 ℃ of hydrolysis 24 hours, 10000rpm, 4 ℃ centrifugal 30 minutes, stay supernatant again; Supernatant is held back with 5000 dalton's hollow fiber column ultrafilter, collects the filtrate that obtains and is the calf blood (protein removed) extracting solution, obtains 1000mL (g) Deporteinnized calf serum extracting solution; After the degerming of extracting solution usefulness microporous filter membrane, lyophilizing 24 hours promptly gets 40g calf serum protein-removing extract dry powder.
2, the mixing of substrate and additive and sterilization
Get benzocaine 250g, sodium carboxymethyl cellulose 680g, pectin 1000g, ethyl hydroxybenzoate 2.5g, Mentholum 50g, liquid paraffin 1250g, white vaseline 1757.5g, behind above-mentioned substrate and additive mix homogeneously, under 121 ℃, autoclaving 25min.
3, the acquisition of calf serum deprotein oral cavity ointment and packing, detection
Get the calf serum protein-removing extract dry powder 10g of step 1 preparation, behind the mixture mixing of step 2, obtain calf serum deprotein oral cavity ointment through sterilizing.With the packing of ointment tucker, be distributed into per 5,10g/ and prop up two kinds of specifications.The calf serum deprotein oral cavity ointment of present embodiment preparation is qualified after testing.
The preparation of embodiment 3, calf serum deprotein oral cavity ointment
Producing 1000 calf serum deprotein oral cavity ointments (calf serum protein-removing extract dry powder content: 0.4%, be equivalent to contain Deporteinnized calf serum extracting solution 10%) detailed process may further comprise the steps:
1, the preparation of calf serum protein-removing extract dry powder
Preparation calf serum protein-removing extract dry powder under aseptic condition, concrete grammar is: adopt the venous blood of the purebred cattle in northeast at 1-6 monthly age, 4000rpm, 4 ℃ of centrifugal 15 minutes separation of serum; Get 5L serum, add 10L 96% ethanol, constantly stir, left standstill 60 minutes, make protein denaturation precipitation, through 20000rpm, 4 ℃ of centrifugal 30min, abandon precipitation then, obtain the 12.5L supernatant, utilize the rotary evaporation in vacuo instrument, 37 ℃, 1 atmospheric pressure decompression removes ethanol; In remaining liquid, add the 1.5g compound protease, 37 ℃ of hydrolysis 24 hours, 10000rpm, 4 ℃ centrifugal 30 minutes, stay supernatant again; Supernatant is held back with 5000 dalton's hollow fiber column ultrafilter, collects the filtrate that obtains and is the calf blood (protein removed) extracting solution, obtains 1000mL (g) Deporteinnized calf serum extracting solution; After the degerming of extracting solution usefulness microporous filter membrane, lyophilizing 24 hours promptly gets 20g calf serum protein-removing extract dry powder.
2, the mixing of substrate and additive and sterilization
Get benzocaine 250g, sodium carboxymethyl cellulose 640g, pectin 1100g, ethyl hydroxybenzoate 2g, Mentholum 40g, liquid paraffin 1350g, white vaseline 1598g, behind above-mentioned substrate and additive mix homogeneously, under 121 ℃, autoclaving 30min.
3, the acquisition of calf serum deprotein oral cavity ointment and packing, detection
Get the calf serum protein-removing extract dry powder 20g of step 1 preparation, behind the mixture mixing of step 2, obtain calf serum deprotein oral cavity ointment through sterilizing.With the packing of ointment tucker, be distributed into per 5,10g/ and prop up two kinds of specifications.The calf serum deprotein oral cavity ointment of present embodiment preparation is qualified after testing.
The influence that embodiment 4, detection calf serum deprotein oral cavity ointment heal to the rabbit oral ulcer
Medicine:
Be subjected to the calf blood (protein removed) oral cavity ointment of reagent product: embodiment 1,2,3 preparations, specification be 5 the gram/, the content of calf serum protein-removing extract dry powder was respectively for 0.1%, 0.2%, 0.4% (being equivalent to contain calf serum protein-removing extract 2.5%, 5%, 10%).
The positive control medicine: commercially available peaceful health mouth hose lining, specification be 3.5 the gram/.
Laboratory animal: 20 of health large ear rabbits, body weight 2.0kg-2.5kg, male, female all can (doe be the unpregnancy person).
Rabbit oral ulcer replication of Model: list of references (Xiong Fengqin, Li Shanhua, kingly way etc. oral ulcer medicine film is to the therapeutical effect of the experimental oral ulcer of Cavia porcellus. modern stomatology magazine, 1997,11 (4): 259-260; Chen Ke, Ren Yuguang, Luo Yu rather waits the animal experiment study of .Nd:YAG laser therapy oral ulcer. use laser, 1998,18 (3): the method 135-136) grinds the hole that diameter is 3mm with plastic centrifuge tube bottom, is stained with 90% carbolic acid (moistening, do not overflow be degree) with cotton pellet and puts aperture place in the pipe; Rabbit is used 3% Nembutal vein anesthetic, retract upperlip, expose buccal, oral cavity mucosa, centrifuge tube is perpendicular to oral mucosa, burnt 30 seconds, and made local oral mucosa be pale asphyxia, in wound surface formation next day roun ulcer, the surface is congested, no pseudomembrane, and the border is more clear.Two ulcer surfaces about same rabbit is burnt.
Calf serum deprotein oral cavity ointment with embodiment 1,2,3 preparations is an example, detects the influence of calf serum deprotein oral cavity ointment of the present invention to the healing of rabbit oral ulcer.Concrete experimental technique is as follows: laboratory animal is divided into five groups at random, i.e. three of calf serum deprotein oral cavity ointment test group (basic, normal, high three dosage), positive drug matched group and blank group, every group of 4 rabbits, 8 ulcer surfaces.Carry out following treatment behind the ulcer:
The I group is the blank group, and wound surface is coated with normal saline;
The II group is coated with 0.1% calf serum deprotein oral cavity ointment, every day 4 times for the low dosage test group;
The III group is coated with 0.2% calf serum deprotein oral cavity ointment, every day 4 times for middle dosetest group;
The IV group is coated with 0.4% calf serum deprotein oral cavity ointment, every day 4 times for the high dose test group;
V organizes positive matched group, is coated with peaceful health mouth hose lining, every day 1 time;
Each group continues medication until ulcer healing.
In the therapeutic process, observe and write down the situation index of ulcer area and different time ulcer healing, simultaneously ulcer hyperemia, the edema situation of respectively forming face in the therapeutic process are carried out integration relatively, the integration standard is as follows: do not have hyperemia, edema note 0 minute; Mild hyperaemia, edema note 1 minute; Moderate hyperemia, edema note 2 minutes; Severe hyperemia, edema note 3 minutes; Each is organized data and all represents with means standard deviation, with t check carrying out statistical analysis.Experimental result is as follows:
(1) calf serum deprotein oral cavity ointment is to the influence of ulcer hyperemia, edema degree integration
Burn and local became canescence the same day, form ulcer next day, ulcer contrafluxion, edema are serious, alleviate gradually after the treatment, respectively organize ulcer hyperemia in the treatment, the statistical result of edema degree integrated value is as shown in table 1:
Respectively organize ulcer hyperemia, the statistical result of edema degree integrated value in table 1 treatment
| Group | n | Before the treatment | Treat after 3 days | ||
| Total mark | x±SD | Total mark | x±SD | ||
| Dosage group high dose group positive controls in the blank group low dose group | 8 8 8 8 8 | 24 24 24 24 24 | 3±0 3±0 3±0 3±0 3±0 | 19 13 10 9 12 | 2.38±0.74 1.63±0.52 * 1.25±0.71 ** 1.13±0.64 ** 1.50±0.53 * |
Annotate: compare * P＜0.05, * * P＜0.01 with the blank group.
Treat after 3 days, the total mark of basic, normal, high dosage calf serum deprotein oral cavity ointment group reduced to 13,10,9 fens by 24 minutes before the treatment respectively, and positive controls reduced to 12 fens in preceding 24 minutes by treatment, and the blank group reduced to 19 fens by 24 minutes.The above results shows that calf serum deprotein oral cavity ointment of the present invention can obviously alleviate the congestion and edema degree of ulcer.
(2) calf serum deprotein oral cavity ointment is to the influence of rabbit oral ulcer area
The ulcer area situation of change of each group is as shown in table 2 in the treatment:
The ulcer area situation of change (x ± SDmm of each group of different time in table 2 treatment
2, n=8)
| Group | Before the treatment | Treat after 2 days | Treat after 3 days | Treat after 4 days |
| Dosage group high dose group positive controls in the blank group low dose group | 46.63±1.60 47.10±1.51 46.81±1.61 47.13±1.48 46.78±1.77 | 64.99±0.94 46.64±1.16* 40.58±0.37* 39.98±0.83* 46.35±0.69* | 65.46±0.81 32.75±0.47* 23.44±0.51* 20.63±0.61* 31.44±0.72* | 38.37±0.74 15.52±0.58* 8.93±0.81* 7.16±0.48* 14.47±0.70* |
Annotate: * and matched group be P＜0.01 relatively.
The treatment of middle and high dosage group calf serum deprotein oral cavity ointment after 2 days visible ulcer area obviously dwindle, and prolongation in time acts on more remarkable; Low dose group and positive controls ulcer area after treating 3 days also obviously dwindles, each group is compared difference with the blank group and is had significance meaning (P＜0.01), shows that calf serum deprotein oral cavity ointment of the present invention has healing effect preferably to oral ulcer.
(3) oral cavity ointment is to the influence of rabbit oral ulcer healing time
The oral ulcer healing time statistical result of each experimental group rabbit is as shown in table 3:
Table 3 is respectively organized the statistical result of ulcer healing time
| Group | The total number of ulcer surface | Healing ulcer surface number | The average healing (x ± SDd) | |||
| Treated 4 days | Treated 5 days | Treated 6 days | Treated 7 days | |||
| Dosage group high dose group positive controls in the blank group low dose group | 8 8 8 8 8 | 0 1 5 7 1 | 1 4 3 1 5 | 2 3 1 0 2 | 5 0 0 0 0 | 6.50±0.76 5.25±0.71 a 4.38±0.52 ab 4.13±0.35 ace 5.13±0.64 ad |
Annotate: a and blank group be P＜0.01 relatively, and b and low dose group be P＜0.05 relatively, and c and low dosage be P＜0.01 relatively, and d and low dose group be P＞0.05 relatively, and e and middle dosage group be P＞0.05 relatively.
The middle and high dosage group treatment of calf serum deprotein oral cavity ointment most of healing of ulcer after 4 days, low dose group and positive controls are in treatment also healing substantially of ulcer after 5 days, the The average healing of each group is compared obvious shortening (P＜0.01) with the blank group, show that calf serum deprotein oral cavity ointment of the present invention can obviously shorten the healing time of rabbit oral ulcer, have the effect of tangible promotion oral ulcer healing.
Claims (7)
1. calf serum deprotein oral cavity ointment, it is characterized in that: the raw material for preparing this oral cavity ointment is: calf serum protein-removing extract dry powder 0.1-0.4 part, benzocaine 4-6 part, sodium carboxymethyl cellulose 12.8-14.4 part, pectin 16-24 part, ethyl hydroxybenzoate 0.04-0.06 part, Mentholum 0.8-1.2 part, liquid paraffin 20-30 part, white vaseline 30-40 part.
2. calf serum deprotein oral cavity ointment according to claim 1, it is characterized in that: the raw material for preparing described oral cavity ointment is: 0.2 part in calf serum protein-removing extract dry powder, 5 parts of benzocaine, 13.6 parts of sodium carboxymethyl cellulose, 20 parts of pectin, 0.05 part of ethyl hydroxybenzoate, 1 part of Mentholum, 25 parts of liquid paraffin, 35.15 parts of white vaseline.
3. the preparation method of the described calf serum deprotein oral cavity ointment of claim 1 may further comprise the steps:
1) preparation calf serum protein-removing extract dry powder;
2) take off and state components by weight portion: benzocaine 4-6 part, sodium carboxymethyl cellulose 12.8-14.4 part, pectin 16-24 part, ethyl hydroxybenzoate 0.04-0.06 part, Mentholum 0.8-1.2 part, liquid paraffin 20-30 part, white vaseline 30-40 part; Behind above-mentioned substrate and additive mix homogeneously, sterilization;
3) get calf serum protein-removing extract dry powder 0.1-0.4 part that step 1) prepares, with step 2) behind the mixture mixing of sterilization, obtain calf serum deprotein oral cavity ointment.
4. the preparation method of calf serum deprotein oral cavity ointment according to claim 3, it is characterized in that: the preparation method of calf serum protein-removing extract dry powder is in the described step 1): adopt 1-6 monthly age children cattle venous blood, separation of serum, use the ethanol Deproteinization, ethanol is removed in decompression, in the liquid of remainder, add the compound protein enzyme hydrolysis, centrifugal, stay supernatant, supernatant is carried out ultrafiltration hold back, collecting the filtrate that obtains is the calf blood (protein removed) extracting solution, after the degerming, lyophilizing obtains calf blood protein-removed extraction dry powder.
5. the preparation method of calf serum deprotein oral cavity ointment according to claim 4, it is characterized in that: described young cattle breeds is the purebred cattle in northeast; During with the ethanol Deproteinization, used ethanol is the ethanol of quality percentage composition 96%, and the ethanol consumption is the twice of serum volume; Alcoholic acid method is removed in decompression: use the rotary evaporation in vacuo instrument, 37 ℃, 1 atmospheric pressure decompression removes ethanol; Time with the compound protein enzyme hydrolysis is 20-28 hour; Adding the centrifugal condition of the laggard row of compound protein enzyme hydrolysis is: 8000-12000rpm, 4 ℃, 20-40 minute; It is 4000-6000 dalton that supernatant is carried out the aperture that ultrafiltration cuts used hollow fiber column.
6. the preparation method of calf serum deprotein oral cavity ointment according to claim 5 is characterized in that: the time of described hydrolysis with compound protease is 24 hours; Adding the centrifugal condition of the laggard row of compound protein enzyme hydrolysis is: 10000rpm, 4 ℃, 30 minutes; It is 5000 dalton that supernatant is carried out the aperture that ultrafiltration cuts used hollow fiber column.
7. the preparation method of calf serum deprotein oral cavity ointment according to claim 3, it is characterized in that: the addition of various substrate and additive is described step 2): 5 parts of benzocaine, 13.6 parts of sodium carboxymethyl cellulose, 20 parts of pectin, 0.05 part of ethyl hydroxybenzoate, 1 part of Mentholum, 25 parts of liquid paraffin, 35.15 parts of white vaseline; The condition that substrate and additive agent mixture are sterilized is: under 121 ℃, and autoclaving 20-30min.
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| CN102309522B (en) * | 2011-09-23 | 2013-02-13 | 沈阳斯佳科技发展有限公司 | Deproteinised porcine blood extract oral paste and preparation technology thereof |
| CN106727893A (en) * | 2016-12-01 | 2017-05-31 | 四川好医生攀西药业有限责任公司 | Pharmaceutical composition for radiation stomatitis and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1183280A (en) * | 1996-11-27 | 1998-06-03 | 锦州医学院科技开发部 | Method for extracting deproteinized calf blood extract |
| CN1586499A (en) * | 2004-07-08 | 2005-03-02 | 合肥兆峰科大药业有限公司 | Calf blood deproteinizing extract, its eye ointment preparation and its preparing method |
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2005
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1183280A (en) * | 1996-11-27 | 1998-06-03 | 锦州医学院科技开发部 | Method for extracting deproteinized calf blood extract |
| CN1586499A (en) * | 2004-07-08 | 2005-03-02 | 合肥兆峰科大药业有限公司 | Calf blood deproteinizing extract, its eye ointment preparation and its preparing method |
Non-Patent Citations (2)
| Title |
|---|
| 小牛去蛋白提取物对大鼠深二度烧伤创面愈合作用的研究. 王金华等.锦州医学院学报，第6卷第25期. 2004 * |
| 小牛血去蛋白提取物对大鼠实验性胃溃疡的疗效观察. 才宁等.中国药理学通报，第8卷第19期. 2003 * |
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Address after: 121013 Songshan street, Taihe District, Jinzhou, Liaoning Province, No. 55 Patentee after: Aohong Pharmaceutical Co., Ltd., Jinzhou Address before: 121013 No. 10 West exposed street, Taihe District, Liaoning, Jinzhou Patentee before: Aohong Pharmaceutical Co., Ltd., Jinzhou |