CN104415212A - Application of composition of garlic total polysaccharide and sulfur-containing amino acid in resisting skin desquamation and itching - Google Patents
Application of composition of garlic total polysaccharide and sulfur-containing amino acid in resisting skin desquamation and itching Download PDFInfo
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- CN104415212A CN104415212A CN201310376541.XA CN201310376541A CN104415212A CN 104415212 A CN104415212 A CN 104415212A CN 201310376541 A CN201310376541 A CN 201310376541A CN 104415212 A CN104415212 A CN 104415212A
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- China
- Prior art keywords
- sulfur
- amino acid
- containing amino
- bulbus allii
- garlic
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Links
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- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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Abstract
The invention provides a composition of garlic total polysaccharide and sulfur-containing amino acid. The composition mainly comprises garlic water-soluble macromolecule effective components, has good effects on inhibiting scurf and relieving itching, is applicable to various skin desquamation, scurf, ichthyosis, and various skin itching symptoms, and has the functions of supporting right, dispelling evil, and reducing toxicity. The composition has wide application prospects, and can be prepared into medicaments or cosmetics for preventing and treating desquamation and relieving itching with significant curative effect and less toxic or side effects. The invention also provides a method for preparing the composition, which comprises: extracting garlics, performing centrifugation or filtration to remove superfluous impurities so as to obtain the composition meeting the requirements; or purifying the extract through an ion exchange chromatographic column to obtain garlic sulfur-containing amino acid; then performing concentration and alcohol precipitation of an eluate to obtain garlic total polysaccharide, or adding the extract into an organic solvent such as alcohol and the like, and performing precipitation to obtain garlic polysaccharide; finally combining the garlic sulfur-containing amino acid with the polysaccharide according to a weight ratio to obtain the composition meeting the requirements. The preparation method is simple in operation, low in cost, controllable in quality, and suitable for industrialization.
Description
Technical field
The invention belongs to field of medicaments, relate to and a kind ofly comprise the garlic polysaccharide compositions that become be grouped into water miscible with Bulbus Allii sulfur-containing amino acid class, specifically, relate to the compositions that comprises garlic total polysaccharide and Bulbus Allii total amino acids prevents in desquamation and pruritus medicine and cosmetics application in preparation, and the preparation method of said composition.
Background technology
Desquamation (desquamation) is common in the continuous keratinization in normal skin top layer and renewal, but due to quantity little, generally not easily discover.Visible a large amount of desquamation under pathological state.Rice defurfuration is common in measles; Lamellar desquamation is common in scarlet fever; Silvery white squamous desquamation sees psoriasis.
Normal skin epidermis is the keratinized stratified squamous epithelium of abrasion-resistant, the keratinocyte of the prickle cell layer of spinous layer strengthens intercellular connection with intercellular bridge on the one hand, strengthens intercellular bonding force on the other hand between the keratinocyte of cutin deep layer with lipid Membrane cover.The epidermis in this stage has the barrier function of stronger resistance to mechanical power and chemical lesion.Lamellar body includes the lipoid of 2/3 and the protein enzyme of 1/3, when cutin deep layer keratinocyte is to surface migration and when changing cutin shallow-layer keratinocyte into, its intercellular bridge and lipid Membrane cover need participate in degraded through hydrolytic enzyme such as iuntercellular lamellar bodies can normal desquamation.
The phenotype of abnormal desquamation patient presents does not dissociate between keratinocyte and piles up for lamellar squama.Flaggy ichthyosis patient is recessive inheritance's keratinocyte Developmental and Metabolic Disorder, psoriasis is the keratinocyte Developmental and Metabolic Disorder of immunogenicity inflammation, the Developmental and Metabolic Disorder of two keratinocytes all can cause apoptosis incomplete, presenting the ichthyotic pathogenic factor of the different flaggy of desquamation is Cholesterol sulfate vinegar enzyme genetic flaw, cause Cholesterol sulfate vinegar can not normal cleavage, occur abnormal desquamation
Desquamation is usually along with pruritus, and pruritus is the main subjective symptoms of a lot of common skin diseases.But often dysphoria, misery can be brought hard to bear to people.Desquamation and pruritus are a kind of clinical symptoms of obstinate refractory gloomy, be referred to as pruritus only having general or regional pruritus without the dermatosis that visible primary cutaneous is damaged clinically, current front home and abroad lacks to have scratched where it itches effective treatment way to desquamation.Scratch but promote to discharge more histamine, increasing the weight of gargalesthesia, cause even more serious skin injury and inflammation.Present Therapeutic Method takes Claritin mostly usually, oral or smear Hangzhoupro fungus medicine outward, smear adrenocortical hormones external preparation etc.Though said medicine has certain curative effect, oral drugs and external hormone medicine, also have many side effect, can not solve a basic desquamation difficult problem.
The medicine for external use of the antipruritic alleviating pain and detumescence that Chinese patent 02,138,752 1 kinds of pure Chinese medicines are made, it is made up of sulfur, Realgar, Bulbus Allii, Fructus Zanthoxyli, Radix Sophorae Flavescentis, the Radix Stemonae, its each component sulfur: Realgar: Bulbus Allii: Fructus Zanthoxyli: Radix Sophorae Flavescentis: the molecular proportion of the Radix Stemonae is that 1:0.5-5:0.2-4:0.2-5:1-4:1-5 it provides a kind of applicable various crowd's use, to poisonous insect such as treatment mosquito etc. bite there is special result antipruritic, pain relieving, the special medicine for external use of detumescence, this patent is only the compound preparation containing Bulbus Allii, does not carry out correlational study to the effective ingredient of Bulbus Allii and effect.
Chinese patent 200410020382 discloses " the antipruritic varnish of Bulbus Allii local topical ", provide the simple preparation technology of the antipruritic extract of Bulbus Allii, deep research is not carried out to the composition of extract and effect, this method has carried out sort research to the composition of Bulbus Allii, ratio optimization is carried out to the amount of sulfur-containing amino acid wherein and the amount of garlic polysaccharide, obtain Bulbus Allii and prevent desquamation and antipruritic two large constituents: sulfur-containing amino acid class and garlic polysaccharide, remove other compositions of Bulbus Allii, improve safety, effectiveness and the quality controllability of this product.This product sulfur amino acids and garlic polysaccharide class interact, and repair skin function, can alleviate the sense of discomfort of patient, patient's desquamation situation is significantly improved, and relieves the pruritus of skin simultaneously.
Chinese patent 201,010,141,440 1 kinds of removing scurf and relieving itching liquid formulas, the mass percent of each component is as follows: mature vinegar 12%-24%, allicin 8%-12%, ethanol 10%-15%, Radix Polygoni Multiflori extracting solution 5%-8%, soft water 50%-60%.The formula of uniqueness of the present invention and effective composition can be good at changing scalp pH value environment, sterilizing and acceleration scalp blood circulation, can percdation root of hair, supplement hair desired nutritional, improve hair quality, thus effectively can remove the dandruff, and suppress the growth of the dandruff, and finally reach antipruritic object.This patent only mentions the liposoluble constituent allicin of Bulbus Allii, and the water soluble ingredient garlic polysaccharide of not mentioned Bulbus Allii and aminoacid.
Document and patent report be Bulbus Allii extract, the preparation method of allicin and effect, the application of garlic polysaccharide and sulfur-containing amino acid class is not related to; Above-mentioned patent just mentions that Bulbus Allii extract has antipruritic effect, but whether elaboration garlic polysaccharide and sulfur-containing amino acid have antipruritic and suppress scurf effect, also ratio optimization is not carried out to the amount of sulfur-containing amino acid wherein and the amount of garlic polysaccharide, just simple crude extract; Therefore, in currently available technology, the Application and Development of the water soluble ingredient such as garlic polysaccharide and sulfur-containing amino acid goed deep into not enough and comprehensively, need to strengthen Bulbus Allii water soluble ingredient in the research preventing desquamation and antipruritic application.
Summary of the invention
An object of invention is to provide a kind of garlic total polysaccharide and sulfur-containing amino acid compositions prevent in desquamation and antipruritic agent application in preparation, and another object of the present invention is to provide the pharmaceutical composition of a kind of garlic total polysaccharide of obtaining according to described preparation method and sulfur-containing amino acid.
The object of the invention is to be achieved through the following technical solutions:
A kind of compositions comprising garlic total polysaccharide and Bulbus Allii sulfur-containing amino acid class is for the preparation of the application prevented and/or treated in the medicine of desquamation and/or pruritus, cosmetics.
Further described desquamation and pruritus are selected from: scalp desquamation and other local pruritus, psoriatic skin desquamation and pruritus, fungal infection desquamation and pruritus, the defurfuration of measles rice and pruritus, scarlatinous lamellar desquamation and pruritus, skin pruritus, diabetic pruritus, uremic pruritus, anemia of pregnant woman's pruritus, mosquito bite and insect bite pruritus and independent pruritus.
Further described medicine is selected from following dosage form: spray, aerosol, liniment, lotion, externally used paste, suppository, gel, injection, tablet, granule, capsule, soft capsule, drop pill, oral liquid, membrane, powder and pill.
Further described compositions is made up of garlic total polysaccharide and the large constituents of Bulbus Allii sulfur-containing amino acid class two.
Further described Bulbus Allii sulfur-containing amino acid class main component comprises: alliin: (S)-3-(pi-allyl sulfenyl)-ALANINE, S allyl cysteine (Sallylcysteine) and S-propene thiol cysteine;
Further described garlic total polysaccharide is heteropolysaccharide, and the composition after its hydrolysis comprises: arabinose, glucose, fructose, galactose, xylose;
The ratio of further garlic total polysaccharide and Bulbus Allii sulfur-containing amino acid class is 50:1 ~ 1:50 by weight.
The ratio of further garlic total polysaccharide and Bulbus Allii sulfur-containing amino acid class counts 20:1 ~ 1:20 with liquid chromatograph peak by normalization method.
Further garlic total polysaccharide and Bulbus Allii sulfur-containing amino acid class the liquid phase chromatogram condition of ratio measuring be: take octadecylsilane chemically bonded silica as filler, take methanol-water as mobility, condition of gradient elution is: 0 minute ~ 10 minutes: water-methanol (99:1), 10 minutes-30 minutes: water-methanol (50:50), detector: evaporative light scattering detector.The chromatographic peak group first eluting chromatographic column is the chromatographic peak polysaccharide of Bulbus Allii sulfur-containing amino acid class, rear elution chromatography post; The relative retention time of the two is 6 ~ 9
On the other hand, the invention provides the pharmaceutical composition of a kind of garlic total polysaccharide of obtaining according to described preparation method and sulfur-containing amino acid.
Described preparation method comprises the following steps:
1) Bulbus Allii obtains Bulbus Allii crude extract through water, ethanol or other organic solvent extraction: by above-mentioned crude extract, by filtering, comprise filter paper, filter membrane and filter cloth etc., centrifuge remove impurity, the aminoacid regulating the pH of extracting solution to make precipitates, through methods such as alcohol precipitate polysaccharides or water precipitation organic impuritiess, in adjustment extracting solution, the ratio of garlic total polysaccharide and Bulbus Allii sulfur-containing amino acid class, makes the ratio of said two devices reach the scope counting 20:1 ~ 1:20 with liquid chromatograph peak by normalization method.
2) Bulbus Allii is through water extraction, obtains Aqueous extracts;
3) macroporous resin crossed by gained medicinal liquid, and washing and/or ethanol are washed successively, collects eluent, and decompression recycling ethanol is to without alcohol taste, dry, obtains brown or faint yellow Bulbus Allii sulfur-bearing alliin class;
4) or by 2) gained extracting solution crosses cationic ion resin, by water or alkaline solution eluting, collects eluent, and decompression recycling ethanol is to without alcohol taste, or concentrate drying, obtains brown or faint yellow Bulbus Allii sulfur-bearing alliin class;
5) by step 3) or 4) other eluents of gained, concentrated, add ethanol or other precipitant precipitation, obtain garlic polysaccharide;
6) by step 1) concentrated after water extraction, add alcohol settling or other precipitant, obtain garlic polysaccharide;
7) by step 3) or 4) gained Bulbus Allii sulfur-containing amino acid class and 5) or 6) gained garlic polysaccharide is mixed to get the compositions of garlic total polysaccharide and Bulbus Allii sulfur-containing amino acid class, makes the ratio of said two devices reach the scope counting 20:1 ~ 1:20 with liquid chromatograph peak by normalization method.
Said composition application and the clinical effect with good removing scurf and relieving itching, age was 25 ~ 55 years old health volunteer, 50 examples, many and the pruritus of the dandruff, all don't work with various shampoo hair washing, uses garlic polysaccharide spray, be sprayed on before hair washing on scalp, repeatedly smear 2 minutes, after January, 75% patient's scalp situation disappears, and dandruff disappears substantially; Psoriasis decortication bits and Pruritus: the age, 10 examples were secondary, for many years ill 25 ~ 55 years old health volunteer, use fungus external preparation, but decortication bits appear in variable interval every day, Pruritus unbearably, sprays this product, every day 3 times, one time 10 spray, repeatedly smears to and is not having pruritus to feel, do not having obvious scurf to produce, 9 routine gargalesthesias its obviously alleviate, the phenomenon that 6 routine scurfs come off is attained the Way improvement very soon.
Accompanying drawing explanation
Below, describe embodiments of the invention in detail by reference to the accompanying drawings, wherein:
Fig. 1 is the liquid chromatogram of embodiment 1 Bulbus Allii sulfur-containing amino acid and garlic polysaccharide ratio
Fig. 2 is the typical liquid chromatographic figure of embodiment 5 Bulbus Allii sulfur-containing amino acid and garlic polysaccharide
Fig. 3 is the total ion current figure of embodiment 5 Bulbus Allii sulfur-containing amino acid and garlic polysaccharide compositions
Fig. 4 is the molecular ion peak spectrogram of embodiment 5 Garlic propylhomoserin
Detailed description of the invention
Below in conjunction with specific embodiment, and comparable data describes in further detail the present invention.Should be understood that these embodiments just in order to demonstrate the invention, but not limit the scope of the invention by any way.Below in an example, the various process do not described in detail and method are conventional methods as known in the art.Source, the trade name of agents useful for same and be necessary to list its constituent person, all indicate when occurring first, identical reagent used if no special instructions thereafter, all identical with the content indicated first.
The filtration preparation method of embodiment 1 garlic polysaccharide and sulfur-containing amino acid
The fresh stem 100g of ripe Bulbus Allii adds water 1000ml, boil 15 minutes, filtered while hot, place 12 hours, filter again and have polysaccharide composition more, make the ratio of the compositions of garlic total polysaccharide and Bulbus Allii sulfur-containing amino acid class reach the scope counting 20:1 ~ 1:20 with liquid chromatograph peak by normalization method.
The centrifugal preparation method of embodiment 2 Bulbus Allii polysaccharide and sulfur-containing amino acid
Ripe garlic stem 100g, pulverizes water 1000ml in stirring at room temperature 6 hours, places and filter, obtain aqueous extract.Room temperature leaves standstill 12 hours, centrifugal disgorging, then filtration has polysaccharide composition more, makes the ratio of the compositions of garlic total polysaccharide and Bulbus Allii sulfur-containing amino acid class reach the scope counting 20:1 ~ 1:20 with liquid chromatograph peak by normalization method.
The precipitate with ethanol preparation method of embodiment 3 Bulbus Allii polysaccharide and sulfur-containing amino acid
Ripe garlic stem 100g, add water 1000ml in, boil 15 minutes, pulverize, filter, obtain aqueous extract.Room temperature place cooling, add ethanol disgorging, more centrifugal or filter have polysaccharide composition more, make the ratio of the compositions of garlic total polysaccharide and Bulbus Allii sulfur-containing amino acid class reach the scope counting 20:1 ~ 1:20 with liquid chromatograph peak by normalization method.Get above-mentioned solution and cross 0.45 μm of microporous filter membrane, obtain the filtrate of clarification, implant in Pressurized sprays bottle, make garlic polysaccharide spray or varnish, or make emulsifiable paste or gel with suitable adjuvant.
The preparation method of the mistake chromatographic column of embodiment 4 Bulbus Allii polysaccharide and sulfur-containing amino acid
Ripe garlic stem 500g, add water 5000ml in, boil 15 minutes, pulverize, filter, obtain aqueous extract.Room temperature places cooling, adds ethanol disgorging, and the drying of precipitation decompress(ion) obtains garlic polysaccharide: paste-forming rate 15.3% is 55% by the cubage of glucose
Ripe garlic stem 500g, add water 5000ml in, boil 15 minutes, pulverize, filter, obtain aqueous extract.Room temperature places cooling, and centrifugal filtration, obtains settled solution, and above-mentioned solution hydrochloric acid adjust pH is equaled 2, cross cation exchange resin by above-mentioned solution, wash with water and take off unnecessary polysaccharide, then add suitable ammonia eluting, eluent is concentrated, dry, obtain Bulbus Allii sulfur-containing amino acid class;
Get garlic polysaccharide and Bulbus Allii amino acids mixes by weight ratio, obtain above-mentioned composition.Said composition being dissolved in suitable solvent, as made spray or varnish in water, ethanol or the two mixed solvent, or making emulsifiable paste or gel with suitable adjuvant.
The quality control of embodiment 5 garlic polysaccharide and sulfur-containing amino acid
Garlic polysaccharide and sulfur-containing amino acid compositions typical case chromatographic peak get above-described embodiment 4 garlic polysaccharide and Bulbus Allii amino acids compositions, dissolve by mobility, cross 0.45 μm of microporous filter membrane, obtain the filtrate of clarification, get above-mentioned solution injection liquid chromatography, obtain the chromatogram of this product
Liquid phase chromatogram condition:
Chromatographic column: waters XTerra C
184.6mm × 150mm;
Elution requirement: gradient elution
| Time (minute) | Aqueous phase (%) | Methanol (%) |
| 0～10 | 99 | 1 |
| 10～30 | 50 | 50 |
Detector: DAD-ELSD
Sample size: 20 μ l
Result: the chromatographic peak group first eluting chromatographic column is Bulbus Allii sulfur-containing amino acid class, the chromatographic peak polysaccharide of rear elution chromatography post, the relative retention time of the two is 6 ~ 9, and the ratio of the compositions of Bulbus Allii total polysaccharides and Bulbus Allii sulfur-containing amino acid class reaches the scope counting 20:1 ~ 1:20 with liquid chromatograph peak by normalization method.
The extraction solution that the total ion current of sulfur-containing amino acid and the molecular ion peak of main component alliin thereof get the various embodiments described above 1 injects liquid phase mass spectrograph, obtain the liquid chromatography mass spectrometric total ion current figure of this product, total particle flow graph intermediate ion chromatographic peak is mainly the quasi-molecular ions of sulfur-containing amino acid class, and garlic polysaccharide is substantially without quasi-molecular ions.
Chromatographic column: waters XTerra C
184.6mm × 150mm;
Elution requirement: gradient elution
| Time (minute) | Aqueous phase (%) | Methanol (%) |
| 0～10 | 99 | 1 |
| 10～30 | 50 | 50 |
Detector: DAD-MS
Sample size: 20 μ l
Result: the total quasi-molecular ions figure being mainly sulfur-containing amino acid class in total particle flow graph, and the molecular ion peak [M+H] of alliin can be produced in the total ion current peak of sulfur-containing amino acid class
+=178.
The release-prevention of embodiment 6 garlic polysaccharide and sulfur-containing amino acid and antipruritic pharmacological action
Release-prevention is tested: get adult healthy 1 grade of Cavia porcellus 10, body weight 200-300g, male and female half and half, reference culture (trichophyton, dog little armful of daughter bacteria, acrothesium floccosum), recover its pathogenicity before experiment and be inoculated in sabouraud's agar (6abrouraud dextrose agarSDA) slant tube culture medium, 260C cultivates, make each visual field of low power lens (10 × 40) microscopy with normal saline after 10 days and embrace the bacteria suspension that subnumber is no less than 20, be placed in 40C refrigerator for subsequent use.After every guinea pig back both sides depilation, after scratching horny layer (raising success ratio of inoculation) with aseptic injection syringe needle, smear bacteria suspension, each 0.1ml, 2 times 1, totally 3 days.Within the 7th day after final vaccination, get inoculation position hair and scurf, it is experimental subject that fungus microscope examination and cultivation are positive.By infecting each administration 0.1g of animal (spraying 10 times) producing scurf, repeatedly smear 2 minutes, every day 2 times, successive administration is after 1 week, and the production of observation scurf, gets infection site hair simultaneously and scurf does fungus microscope examination and cultivation, judge negative conversion rate and bacterium cloudy refunding, the course for the treatment of is 4 weeks.
Result: after garlic polysaccharide spray administration one week, scurf obviously reduces, during the observation scheduling to last surrounding is always issued, animal scurf disappears substantially, to the average negative conversion rate of trichophyton, dog little armful of daughter bacteria and acrothesium floccosum three kinds of skin trichophyton mentagrophytes infections for adding 80%.
Antipruritic test: get adult healthy Cavia porcellus 10, body weight 200-300g, male and female half and half, shaves hair by each group of right back instep of Cavia porcellus before experiment, shaves hair place with fine sandpaper scratch, and to occur that slight oozing of blood is for degree, scratch area is about 0.5cm
2.Each treated animal all sprays with relative medicine 0.05g (5 spray) at right back instep abrasion, after 10 minutes, drip 0.01% histamine phosphate 25wl/ at wound surface place only, pressed 0.02% every 3 minutes later, 0.03%, 0.04%, 0.05% progressive concentration, is only 25ul/ at every turn, until occur that Cavia porcellus later licks metapedes, finally occurring that the histamine phosphate total amount that Cavia porcellus gives when later licking metapedes is explained for causing to itch, recording causing to itch and explaining of more each treated animal, and carrying out statistical analysis.
Result: garlic polysaccharide spray can improve the itch-threshold value of animal to histamine phosphate, compare with bare substrate group and have significant difference (P < 0.01), both explanations have obvious itching-relieving action when coating medicine-feeding.
Claims (12)
1. the compositions comprising garlic total polysaccharide and Bulbus Allii sulfur-containing amino acid class is for the preparation of the application prevented and/or treated in the medicine of desquamation and/or pruritus, cosmetics.
2. application according to claim 1, is characterized in that: described compositions is made up of garlic total polysaccharide and the large class of Bulbus Allii sulfur-containing amino acid class two.
3. application according to claim 2, is characterized in that: the ratio of garlic total polysaccharide and Bulbus Allii sulfur-containing amino acid class is 50:1 ~ 1:50 by weight, and the optimal proportion of garlic total polysaccharide and Bulbus Allii sulfur-containing amino acid class is 10:3 ~ 3 by weight: 7.
4. application according to claim 2, it is characterized in that: the ratio of garlic total polysaccharide and Bulbus Allii sulfur-containing amino acid class counts 20:1 ~ 1:20 with liquid chromatograph peak area by long-pending normalization method, the optimal proportion of garlic total polysaccharide and Bulbus Allii sulfur-containing amino acid class counts 6:4 ~ 4:6 with liquid chromatograph peak area by normalization method.
5. application according to claim 4, it is characterized in that: garlic total polysaccharide and Bulbus Allii sulfur-containing amino acid class the liquid phase chromatogram condition of ratio measuring be: take octadecylsilane chemically bonded silica as filler, take methanol-water as mobility, condition of gradient elution is: 0 minute ~ 10 minutes: water-methanol (99:1), 10 minutes-30 minutes: water-methanol (50:50), detector: evaporative light scattering detector, the chromatographic peak group first eluting chromatographic column is Bulbus Allii sulfur-containing amino acid class, and the chromatographic peak of rear elution chromatography post is polysaccharide; The ratio of the two counts 20:1 ~ 1 with liquid chromatograph peak area by normalization method: 20.
6. the application according to claim 3 or 4, is characterized in that: described Bulbus Allii sulfur-containing amino acid class main component comprises: alliin: (S)-3-(pi-allyl sulfenyl)-ALANINE, S allyl cysteine (Sallylcysteine) and S-propene thiol cysteine.
7. the application according to claim 3,4 or 6, it is characterized in that: described Bulbus Allii sulfur-containing amino acid class is detected by liquid chromatography mass coupling instrument, and this sulfur-containing amino acid class contains (S)-3-(pi-allyl sulfenyl)-ALANINE (alliin) molecular ion peak [M+H]
+=178.
8. application according to claim 1 and 2, is characterized in that: described garlic total polysaccharide is heteropolysaccharide, and the composition after its hydrolysis comprises: arabinose, glucose, fructose, galactose, xylose.
9. application according to claim 1, is characterized in that: described desquamation disease is selected from: psoriasis, scalp desquamation, desquamation, fungal infection desquamation, the defurfuration of measles rice, scarlatinous lamellar desquamation.
10. the application according to claim 1 or 12, is characterized in that: described desquamation and pruritus are selected from: scalp desquamation and other prurituss, psoriatic skin desquamation and pruritus, fungal infection desquamation and pruritus, the defurfuration of measles rice and pruritus, scarlatinous lamellar desquamation and pruritus, senile skin pruritus, diabetic pruritus, uremic pruritus, anemia of pregnant woman's pruritus, mosquito bite and insect bite pruritus.
11. application according to claim 1 and 2, it is characterized in that: described medicine is selected from following dosage form: spray, aerosol, liniment, lotion, externally used paste, suppository, gel, injection, tablet, granule, capsule, soft capsule, drop pill, oral liquid, membrane, powder and pill.
12. application according to claim 1 and 2, a kind of method preparing the compositions comprising garlic total polysaccharide and Bulbus Allii sulfur-containing amino acid class, is characterized in that: described preparation method comprises the following steps:
1) Bulbus Allii obtains Bulbus Allii crude extract through water, ethanol or other organic solvent extraction: by above-mentioned crude extract, by filtering, centrifugal, tune pH precipitates, adjust the ratio of garlic total polysaccharide and Bulbus Allii sulfur-containing amino acid class through precipitate with ethanol or water precipitating, makes the above-mentioned ratio containing garlic total polysaccharide and Bulbus Allii sulfur-containing amino acid class reach the scope counting 20:1 ~ 1:20 with liquid chromatograph peak by normalization method.
2) Bulbus Allii is through water extraction, obtains Aqueous extracts;
3) macroporous resin crossed by gained medicinal liquid, and washing and/or ethanol are washed successively, collects eluent, and decompression recycling ethanol is to without alcohol taste, dry, obtains brown or faint yellow Bulbus Allii sulfur-bearing alliin class;
4) by 2) gained extracting solution crosses cationic ion resin, by water or alkaline solution eluting, collects eluent, and decompression recycling ethanol is to without alcohol taste, or concentrate drying, obtains brown or faint yellow Bulbus Allii sulfur-bearing alliin class;
5) by step 3) or 4) institute's other eluents of technetium, concentrated, add ethanol or other precipitant precipitation, obtain garlic polysaccharide;
6) by step 1) concentrated after water extraction, add alcohol settling or other precipitant, obtain garlic polysaccharide;
7) by step 3) or 4) gained Bulbus Allii sulfur-containing amino acid class and 5) or 6) gained garlic polysaccharide is mixed to get the compositions of garlic total polysaccharide and Bulbus Allii sulfur-containing amino acid class, makes the ratio of said two devices reach the scope counting 20: 1 ~ 1:20 with liquid chromatograph peak by normalization method.
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