CN110062880B - 通过受控样品蒸发制备无损冷冻载网 - Google Patents

通过受控样品蒸发制备无损冷冻载网 Download PDF

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Publication number
CN110062880B
CN110062880B CN201780076600.8A CN201780076600A CN110062880B CN 110062880 B CN110062880 B CN 110062880B CN 201780076600 A CN201780076600 A CN 201780076600A CN 110062880 B CN110062880 B CN 110062880B
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sample
carrier structure
adapter
light
temperature
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CN110062880A (zh
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托马斯·布朗
斯特凡·阿诺尔德
亨宁·施塔尔贝格
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Universitaet Basel
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/42—Low-temperature sample treatment, e.g. cryofixation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/44—Sample treatment involving radiation, e.g. heat

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Length Measuring Devices By Optical Means (AREA)
CN201780076600.8A 2016-10-17 2017-10-17 通过受控样品蒸发制备无损冷冻载网 Active CN110062880B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP16194230.5 2016-10-17
EP16194230 2016-10-17
PCT/EP2017/076467 WO2018073242A1 (en) 2016-10-17 2017-10-17 Lossless cryo-grid preparation by controlled sample evaporation

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CN110062880A CN110062880A (zh) 2019-07-26
CN110062880B true CN110062880B (zh) 2022-09-20

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US (1) US11860071B2 (https=)
EP (1) EP3526579A1 (https=)
JP (1) JP7017565B2 (https=)
CN (1) CN110062880B (https=)
WO (1) WO2018073242A1 (https=)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160015025A1 (en) 2013-03-15 2016-01-21 Regents Of The University Of Minnesota Cryopreservative compostions and methods
US11122796B1 (en) 2016-02-19 2021-09-21 Regents Of The University Of Minnesota Cryoprotection compositions and methods
US11311008B2 (en) 2016-04-19 2022-04-26 Regents Of The University Of Minnesota. Cryopreservation compositions and methods involving nanowarming
US20210293674A1 (en) * 2018-07-23 2021-09-23 Regents Of The University Of Minnesota Systems and methods for cryopreservation of biomaterials
US11035766B2 (en) 2019-02-14 2021-06-15 Nanosoft, Llc. Cryogenic transmission electron microscopy sample preparation
US11703429B2 (en) 2019-02-14 2023-07-18 Nanosoft, LLC Cryogenic transmission electron microscopy sample preparation
WO2020176498A1 (en) 2019-02-28 2020-09-03 Regents Of The University Of Minnesota High throughput system for production and vitrification of biomaterials in cryoprotectant droplets
CN109972207B (zh) * 2019-05-16 2024-03-08 沈阳工程学院 一种磁场可控式液桥生成器
US11101104B2 (en) * 2019-08-30 2021-08-24 Fei Company Multi modal cryo compatible GUID grid
CN110823931B (zh) * 2019-10-16 2020-10-27 中国科学院生物物理研究所 一种冷冻电镜样品制备方法
US12467740B2 (en) 2020-02-13 2025-11-11 Hamamatsu Photonics K.K. Imaging unit and measurement device
EP3869182A1 (en) * 2020-02-19 2021-08-25 FEI Company Device and method for determining a property of a sample that is to be used in a charged particle microscope
CN111929248A (zh) * 2020-08-26 2020-11-13 重庆渝微电子技术研究院有限公司 半导体冷热台
WO2022155306A1 (en) * 2021-01-13 2022-07-21 Wisconsin Alumni Research Foundation Freezing and jacketing gas-phase biomolecules with amorphous ice for electron microscopy
CN113101990B (zh) * 2021-04-13 2022-06-21 广西大学 一种用于微流体集成芯片中流体试剂储存和自触发并延时释放的方法
WO2024061904A1 (en) * 2022-09-20 2024-03-28 Cryowrite Ag Gripper unit and transfer system
WO2025029985A1 (en) * 2023-08-01 2025-02-06 Wisconsin Alumni Research Foundation Compositions and methods for preparation of a cryogenic electron microscopy sample
WO2025036907A1 (en) 2023-08-15 2025-02-20 Cryowrite Ag Method for cooling a sample solution in a pipette, a pipette holding and cooling unit and a cooling plate
WO2025157760A1 (en) * 2024-01-25 2025-07-31 Roche Diagnostics International Ag Methods and systems for preparing a sample on a substrate

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1478920A (zh) * 2002-03-25 2004-03-03 ���׿ƹɷ����޹�˾ 光学薄膜厚度控制方法及装置，绝缘多层薄膜及制造装置
CN1605566A (zh) * 1998-08-19 2005-04-13 昭和电工株式会社 无定形含钛的细微颗粒物质及包括由其形成的薄膜的制品
CN104198242A (zh) * 2014-08-28 2014-12-10 中国科学院生物物理研究所 聚焦离子束法制备冷冻含水切片的样品夹持工具和转移盒子
CN104215580A (zh) * 2014-09-23 2014-12-17 中国科学技术大学 用于冷冻光电关联显微成像的方法和装置
CN104561229A (zh) * 2015-01-19 2015-04-29 中国人民解放军第三七一医院 一种纳米细菌的培养与形态学鉴定方法
EP3018467A1 (en) * 2014-11-07 2016-05-11 Linkam Scienctific Instruments Ltd. Microscopic sample preparation

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0490721A (ja) 1990-08-06 1992-03-24 Matsushita Electric Ind Co Ltd 収納家具
JPH0649952U (ja) * 1992-12-14 1994-07-08 石川島播磨重工業株式会社 塗工液の膜厚さ測定装置
JP3221797B2 (ja) * 1994-06-14 2001-10-22 株式会社日立製作所 試料作成方法及びその装置
US9355813B2 (en) 2010-03-24 2016-05-31 Brown University Microfluidic blotless cryo TEM device and method
EP2381236A1 (en) 2010-04-23 2011-10-26 Fei Company Apparatus for preparing a cryogenic TEM specimen
EP2805143A4 (en) * 2012-01-17 2015-08-12 Scripps Research Inst DEVICE AND METHOD FOR PRODUCING SAMPLES FOR ELECTRONIC MICROSCOPY
EP2853847B1 (en) 2013-09-30 2016-02-03 Fei Company Preparation of cryogenic sample for charged-particle microscopy
EP3320322B1 (en) * 2015-07-06 2023-05-10 Universität Basel Lossless cryo-grid preparation stage for high-resolution electron microscopy

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1605566A (zh) * 1998-08-19 2005-04-13 昭和电工株式会社 无定形含钛的细微颗粒物质及包括由其形成的薄膜的制品
CN1478920A (zh) * 2002-03-25 2004-03-03 ���׿ƹɷ����޹�˾ 光学薄膜厚度控制方法及装置，绝缘多层薄膜及制造装置
CN104198242A (zh) * 2014-08-28 2014-12-10 中国科学院生物物理研究所 聚焦离子束法制备冷冻含水切片的样品夹持工具和转移盒子
CN104215580A (zh) * 2014-09-23 2014-12-17 中国科学技术大学 用于冷冻光电关联显微成像的方法和装置
EP3018467A1 (en) * 2014-11-07 2016-05-11 Linkam Scienctific Instruments Ltd. Microscopic sample preparation
CN104561229A (zh) * 2015-01-19 2015-04-29 中国人民解放军第三七一医院 一种纳米细菌的培养与形态学鉴定方法

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Publication number Publication date
JP2019537709A (ja) 2019-12-26
EP3526579A1 (en) 2019-08-21
WO2018073242A1 (en) 2018-04-26
CN110062880A (zh) 2019-07-26
JP7017565B2 (ja) 2022-02-08
US11860071B2 (en) 2024-01-02
US20190250078A1 (en) 2019-08-15

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