CN110055249B - 降低THEM6基因表达的siRNA、重组载体及其应用 - Google Patents
降低THEM6基因表达的siRNA、重组载体及其应用 Download PDFInfo
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- CN110055249B CN110055249B CN201910209397.8A CN201910209397A CN110055249B CN 110055249 B CN110055249 B CN 110055249B CN 201910209397 A CN201910209397 A CN 201910209397A CN 110055249 B CN110055249 B CN 110055249B
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- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
- C12N2740/15041—Use of virus, viral particle or viral elements as a vector
- C12N2740/15043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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Abstract
Description
试剂 | 体积(μl) |
ddH<sub>2</sub>O | 41 |
10×反应缓冲液 | 5 |
纯化的GV493质粒DNA(1μg/μL) | 2 |
AgeI(10U/μl) | 1 |
EcoRI(10U/μl) | 1 |
Total | 50 |
试剂 | 体积(μl) |
线性化载体(100ng/μl) | 1 |
双链DNA(100ng/μl) | 1 |
10×T4 DNA连接酶缓冲液 | 2 |
T4 DNA连接酶 | 1 |
双蒸水(ddH<sub>2</sub>O) | 补足至20 |
Claims (3)
Priority Applications (1)
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CN201910209397.8A CN110055249B (zh) | 2019-03-19 | 2019-03-19 | 降低THEM6基因表达的siRNA、重组载体及其应用 |
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CN201910209397.8A CN110055249B (zh) | 2019-03-19 | 2019-03-19 | 降低THEM6基因表达的siRNA、重组载体及其应用 |
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Publication Number | Publication Date |
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CN110055249A CN110055249A (zh) | 2019-07-26 |
CN110055249B true CN110055249B (zh) | 2021-10-15 |
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CN101437536A (zh) * | 2006-03-23 | 2009-05-20 | 诺华有限公司 | 抗肿瘤细胞抗原抗体治疗 |
CN104471402A (zh) * | 2012-04-13 | 2015-03-25 | 鹿特丹伊拉斯谟大学医疗中心 | 用于三阴性乳腺癌的生物标志 |
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Effective date of registration: 20220530 Address after: No.1 Huanghai Road, Shuiji sub district office, Laixi City, Qingdao City, Shandong Province 266600 Patentee after: Qingdao Yanding Biomedical Technology Co.,Ltd. Address before: 2081, building a, 88 Jianghai West Road, Liangxi District, Wuxi City, Jiangsu Province, 214000 Patentee before: Wuxi Xiangyuan Information Technology Co.,Ltd. Effective date of registration: 20220530 Address after: 2081, building a, 88 Jianghai West Road, Liangxi District, Wuxi City, Jiangsu Province, 214000 Patentee after: Wuxi Xiangyuan Information Technology Co.,Ltd. Address before: 224008 no.283, Jiefang South Road, Yancheng City, Jiangsu Province Patentee before: JIANGSU VOCATIONAL College OF MEDICINE |
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Denomination of invention: SiRNA, Recombinant Vector and Their Applications for Reducing the Expression of THEM6 Gene Effective date of registration: 20230721 Granted publication date: 20211015 Pledgee: Weihai commercial bank Limited by Share Ltd. Qingdao branch Pledgor: Qingdao Yanding Biomedical Technology Co.,Ltd. Registration number: Y2023980049237 |
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