CN110051939A - It is a kind of to motivate cell system based on ultrasonics and the bimodal of electromagnetism - Google Patents
It is a kind of to motivate cell system based on ultrasonics and the bimodal of electromagnetism Download PDFInfo
- Publication number
- CN110051939A CN110051939A CN201910332267.3A CN201910332267A CN110051939A CN 110051939 A CN110051939 A CN 110051939A CN 201910332267 A CN201910332267 A CN 201910332267A CN 110051939 A CN110051939 A CN 110051939A
- Authority
- CN
- China
- Prior art keywords
- module
- cell
- excitation
- bimodal
- ultrasonics
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
- A61N2/00—Magnetotherapy
- A61N2/002—Magnetotherapy in combination with another treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
- A61N2/00—Magnetotherapy
- A61N2/004—Magnetotherapy specially adapted for a specific therapy
- A61N2/006—Magnetotherapy specially adapted for a specific therapy for magnetic stimulation of nerve tissue
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
- A61N2/00—Magnetotherapy
- A61N2/02—Magnetotherapy using magnetic fields produced by coils, including single turn loops or electromagnets
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
- A61N7/00—Ultrasound therapy
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
- A61N7/00—Ultrasound therapy
- A61N2007/0004—Applications of ultrasound therapy
- A61N2007/0021—Neural system treatment
- A61N2007/0026—Stimulation of nerve tissue
Abstract
It is a kind of to motivate cell system based on ultrasonics and the bimodal of electromagnetism, belong to field of medical device.The system is made of power module, signal generating module, drive amplification module, ultrasonic action module, magnetic pumping module and detection module.Pulse signal is sent to drive amplification module by signal generating module；Pulse signal is conveyed directly to ultrasonic action module after the processing of drive amplification module and detects signaling module；Detect quality of the signaling module for detection signal output at any time；Power module is ultrasonic action module and magnetic pumping module for power supply；Magnetic pumping module generates magnetic field and module collective effect is motivated by ultrasound and operates in the excitation of cell.The present invention overcomes the limitations of single modal excitation cell, can further improve the proliferative amount and activity of cell, to provide enough and active good seed cells for autotransplantation；More hope are provided for the treatment of traumatic paraplegia.
Description
Technical field
Cell system is motivated based on ultrasonics and the bimodal of electromagnetism the present invention relates to a kind of, belongs to Medical Devices neck
Domain.
Background technique
The wound that spinal cord injury (Spinal Cord Injury, SCI) is the most serious as a kind of orthopaedics, complication is most
Property disease, the crippling, death rate is high and the treatment method of SCI is extremely limited, and cure rate is extremely low.Spinal cord is damaged
Wound, mesenchymal stem cell (Bone mesenchymal stem cells, BMSCs) have great application potential, it can
To bring positive effect to SCI patient in all respects.After spinal cord injury, there are many effect, examples to nerve regneration by the BMSCs of transplanting
Such as immunological regulation and prevent neuroglial formation；It can promote the integration of graft and host tissue, and pass through cell factor
Regenerative process is supported in secretion with growth factor；A large amount of NTFs can be secreted, axon regeneration is promoted and reduces the formation of chamber,
And improve the ability of the part SCI self-regeneration.
The versatility of BMSCs makes it have substitution or repairs the treatment prospect of damage.Marrow is relatively easily obtained, bypasses
The ethics problem that embryonic stem cell uses.Due to its practicability, and other stem cells can be converted to, BMSCs is considered as
Another source of other kinds of stem cell.Acquisition can be induced with BMSCs when necessary, and these are used for special therapeutic method
Stem cell.Meanwhile there is also limitations for the clinical application of BMSCs: it is generally believed that need to select the BMSCs that extracts self into
Property body in skin grafing and mending SCI, but in marrow be mostly candidate stem cell, BMSCs accounting very little only accounts for 0.001%-0.01%.
For SCI patient, autotransplantation is directly only carried out by the BMSCs collected in marrow, cell concentration probably has deficiency.Therefore have must
By small number of BMSCs in vitro subculture, massive amplification is to meet transplanting needs.Meanwhile for cell
Skin grafing and mending SCI, how to improve the vigor of transplanted cells is that transplanting is successfully crucial.
It is an emerging hot spot that physical stimulation, which intervenes stem cell, have it is easy to operate, can quantify, Small side effects, positioning it is quasi-
The advantages that true.Intervene stem cell by physical stimulation, cell transplantation reparation SCI is carried out after the seed cell optimized and is had more
Safety, also more potential applicability in clinical practice.Low-frequency pulse ultrasound (Low intensity pulsed ultrasound, LIPUS)
And low frequency electromagnetic field (Low frequency electromagnetic fields, LFEMF) is used as the physical stimulation factor, is
Promote effective supplementary means of cell Proliferation and differentiation, but cell concentration after motivating and cell activity cannot be guaranteed.
Summary of the invention
The present invention for stem cell excitation propose it is a kind of based on ultrasonics and the bimodal of electromagnetism excitation cell system,
It can be realized and greatly promote cell proliferation and differentiation under synergistic effect on the scene and further increase the technology of cell viability.
1, it is based on above-mentioned purpose, the technical solution that the present invention takes comprises the steps of:
S1, power module provide power supply for drive amplification module, provide driving for magnetic pumping module；
S2, signal generating module issue pulse signal；
S3, the pulse signal generated by step S2 are amplified by drive amplification module；
S4, ultrasonic action module and detection module are sent to by the pulse signal that step S3 amplifies；
S5, ultrasonic action module generate ultrasonic field after step S3, and magnetic pumping module generates electromagnetism after step S1
, ultrasonic wave and electromagnetic field collective effect are operated in the excitation of cell, influence the proliferation and differentiation of cell；
S6, the cell after step S5 excitation is detected；
S7, will be in the cell transplantation after excitation to rat body；
S8, it evaluates by the rat of step S7；
S9, repair SCI's according to the Bone Marrow Mesenchymal Stem Cells Transplantation after the evaluation optimization of the evaluation result of step S6 and S8
Effect.
Above-mentioned S6 carries out detection to the cell after step S5 excitation
S61, cell bio-activity is detected by Cell Counting Kit-8 (CCK-8) kit；
S62, the variation of TUNEL kit detection Apoptosis situation is utilized；
S63, cell NTFs, including BDNF, NGF and NT- are analyzed by detected by Western blot (Western blot, WB)
3 expression variation.
Above-mentioned S8 carries out evaluation to the rat by step S7
S81, with BBB Scale assessment rat hindlimb motor function；
S82, spinal cord center rat Histological state is damaged with HE dyeing, Immunohistochemical Method evaluation；
S83, NTFs expression at damage spinal cord is evaluated with ELISA.
2, two ways sending can be used in the signal generating module, the first is by the more of computer software control
Performance data capture card emits pulse signal, and second is to provide desired signal using random waveform/function signal generator.
3, the drive amplification module is realized with amplifier circuit, utilizes inverting amplifier and voltage follow
Device, can guarantee to put input signal and be twice and be converted into four tunnel amplified signals and be capable of providing stable output.
4, the ultrasonic action module is the ultrasonic transducer module customized according to demand, using ultrasonic probe,
Its centre frequency is 1MHz, and field angle (2 θ -3dB): 15 °, an end connector is bnc interface, a length of 2m of cable, impedance 585
Ω, direct capacitance 1.1nF, enclosure material are stainless steel.
5, the detection module is to carry out signal detection to amplified signal using oscillograph, if it find that abnormal
It can correct immediately, guarantee the normal table work of ultrasonic action module.
6, the magnetic pumping module is made of coil, and the type that different coils generate magnetic field is different.
7, described to motivate cell system, including low-frequency pulse ultrasonic action system based on ultrasonics and the bimodal of electromagnetism
System and low frequency electromagnetic field excitation system two parts.
The advantage of the invention is that motivating cell simultaneously using ultrasonic technology and electromagnetic field technology both modalities which, overcome
Single mode motivates the limitation of cell, further increases the proliferative amount and proliferation activity of cell, to provide for autotransplantation
Enough and active good seed cells；More hope are provided for the treatment of traumatic paraplegia.
Detailed description of the invention
Fig. 1 is systematic schematic diagram of the invention；
Fig. 2 be in the present invention bimodal excitation cell device structure A (device include ultrasonic probe, unicoil, objective table,
Bracket and pedestal)；
Fig. 3 is that (device includes ultrasonic probe, Helmholtz coil, load to bimodal excitation cell device structure B in the present invention
Object platform, bracket and pedestal)；
Fig. 4 be in the present invention bimodal excitation cell device structure C (device include three ultrasonic probes, three groups of unicoils,
Objective table, bracket and pedestal).
Specific embodiment
Technical solution in the present invention is described in further details below with reference to attached drawing of the invention.It only describes below
Property language and be not restrictive language, cannot be as according to limiting the scope of protection of the present invention.
The present invention overcomes the limitations that cell Proliferation quantity when single modal excitation cell and proliferation activity are inadequate, propose
It is a kind of cell system is motivated based on ultrasonics and the bimodal of electromagnetism, the proliferative amount and proliferation that can further improve cell are lived
Property, to provide enough and active good seed cells for autotransplantation；It is provided more for the treatment of traumatic paraplegia
Hope.
Specific implementation step is as follows:
1, a kind of to motivate cell system based on ultrasonics and the bimodal of electromagnetism, it is main to wrap if Fig. 1 is systematic schematic diagram
Include low-frequency pulse ultrasonic action two parts of system and low frequency electromagnetic field excitation system, middle-low frequency pulse be motivated by ultrasound system by
Power module, signal generating module, drive amplification module, ultrasonic action module and detection module composition, low frequency electromagnetic field excitation
System is made of power module, magnetic pumping module.Fig. 2 is that bimodal motivates cell device structure chart, mainly includes ultrasonic probe
(ultrasonic action device), coil (magnetic actuation means), objective table, bracket.Fig. 3 magnetic actuation means are by round Helmholtz coil
Composition, Fig. 4 driver unit are motivated by ultrasound block combiner with three groups by three groups of coils and form, wherein ultrasonic action module is according to need
The ultrasonic transducer module of customization is sought, magnetic actuation means are made of three groups of circular coil.
2, signal generating module is that pulse signal is sent to drive amplification module；Power module is that drive amplification module mentions
Power supply source；Pumping signal is conveyed directly to ultrasonic action module after the processing of drive amplification module and detects signal mode
Block；Detect quality of the signaling module for detection signal output at any time；Ultrasonic action module is directly made after receiving input signal
Excitation for cell operates.
3, wherein ultrasonic-frequency power supply module is the triple channel HT2332 controllable direct current power supply power supply unit produced using Heng Ji company,
Signal generating module is occurred using AFG3052C type random waveform/function signal of U.S. Tyke (Tektronix) company production
Device, drive amplification module use the THS4062 chip of Texas Instrument's production, and detection module is public using U.S. Tyke Tektronix
Take charge of the one TektronixTDS 2024C oscillograph of the six directions of production.
4, magnetic pumping power module provides power supply for magnetic pumping module, and wherein AC power driving coil generates alternation
Magnetic field, pulse current power supply driving coil generate pulsed magnetic field, the magnetic field and ultrasound that magnetic pumping module generates after power drives
Collective effect is operated in the excitation of cell, and wherein unicoil can pass through the distance between regulating winding and objective table after generating magnetic field
It obtains the magnetic field strength needed and motivates cell；Helmholtz coil middle position can produce uniform magnetic field excitation cell；Three
Coil generates magnetic field and motivates to the different parts of mouse living, and energized position is more accurate, and arousal effect is more obvious.
5, bimodal passes through the detection BMSCs life of Cell Counting Kit-8 (CCK-8) kit after intervening cell jointly
Object activity.
6, the variation of BMSCs Apoptosis situation after being intervened using the detection of TUNEL kit.
7, cell NTFs after being intervened by detected by Western blot (Western blot, WB) analysis, including BDNF, NGF
Expression with NT-3 changes.
8, big with HE dyeing, Immunohistochemical Method evaluation damage spinal cord center with BBB Scale assessment rat hindlimb motor function
Rat tissue state, ELISA evaluation damage NTFs expression at spinal cord, are inquired into this, evaluate BMSC cell transplantation reparation SCI
Effect.
It is only used to illustrate the technical scheme of the present invention above, rather than its limitations；Although being carried out referring to aforementioned to the present invention
Detailed description, those skilled in the art should understand that: its still can to aforementioned documented technical solution into
Row modification, or equivalent substitution of some or all of the technical features.
Claims (9)
1. a kind of motivate cell system based on ultrasonics and the bimodal of electromagnetism, it is characterised in that comprise the steps of:
S1, power module provide power supply for drive amplification module, provide driving for magnetic pumping module；
S2, signal generating module issue pulse signal；
S3, the pulse signal generated by step S2 are amplified by drive amplification module；
S4, ultrasonic action module and detection module are sent to by the pulse signal that step S3 amplifies；
S5, ultrasonic action module generate ultrasonic field after step S3, and magnetic pumping module generates electromagnetic field after step S1, surpasses
Sound wave and electromagnetic field collective effect are operated in the excitation of cell, influence the proliferation and differentiation of cell；
S6, the cell after step S5 excitation is detected；
S7, will be in the cell transplantation after excitation to rat body；
S8, it evaluates by the rat of step S7；
S9, the effect that SCI is repaired according to the Bone Marrow Mesenchymal Stem Cells Transplantation after the evaluation optimization of the evaluation result of step S6 and S8.
2. according to claim 1 a kind of based on ultrasonics and the bimodal of electromagnetism excitation cell system, feature exists
In S6 the following steps are included:
S61, cell bio-activity is detected by Cell Counting Kit-8 (CCK-8) kit；
S62, the variation of TUNEL kit detection Apoptosis situation is utilized；
S63, cell NTFs, including BDNF, NGF and NT-3 are analyzed by detected by Western blot (Western blot, WB)
Expression variation.
3. according to claim 1 a kind of based on ultrasonics and the bimodal of electromagnetism excitation cell system, feature exists
In S8 the following steps are included:
S81, with BBB Scale assessment rat hindlimb motor function；
S82, spinal cord center rat Histological state is damaged with HE dyeing, Immunohistochemical Method evaluation；
S83, NTFs expression at damage spinal cord is evaluated with ELISA.
4. according to claim 1 a kind of based on ultrasonics and the bimodal of electromagnetism excitation cell system, feature exists
In: the signal generating module can be used two ways and issue, the first is the multi-functional number by computer software control
Emit pulse signal according to capture card, second is to provide desired signal using random waveform/function signal generator.
5. according to claim 1 a kind of based on ultrasonics and the bimodal of electromagnetism excitation cell system, feature exists
In: the drive amplification module is realized with amplifier circuit, and inverting amplifier and voltage follower, Neng Goubao are utilized
Card, which puts input signal, to be twice and is converted into four tunnel amplified signals and be capable of providing stable output.
6. according to claim 1 a kind of based on ultrasonics and the bimodal of electromagnetism excitation cell system, feature exists
In: the ultrasonic action module is the ultrasonic transducer module customized according to demand, using ultrasonic probe, center frequency
Rate is 1MHz, and field angle (2 θ -3dB): 15 °, an end connector is bnc interface, and a length of 2m of cable, impedance is 585 Ω, Static Electro
Holding is 1.1nF, and enclosure material is stainless steel.
7. according to claim 1 a kind of based on ultrasonics and the bimodal of electromagnetism excitation cell system, feature exists
In: the detection module is to carry out signal detection to amplified signal using oscillograph, if it find that abnormal can be immediately
Amendment guarantees the normal table work of ultrasonic action module.
8. according to claim 1 a kind of based on ultrasonics and the bimodal of electromagnetism excitation cell system, feature exists
In: magnetic pumping module is made of coil, and magnetic pumping module generates magnetic field and ultrasonic collective effect in cell after power drives
Excitation operation, wherein it is strong to generate the magnetic field that can obtain needs by the distance between regulating winding and objective table behind magnetic field for unicoil
Degree excitation cell；Helmholtz coil middle position can produce uniform magnetic field excitation cell；Three coils generate magnetic field to work
The different parts of mouse are motivated, and energized position is more accurate, and arousal effect is more obvious.
9. according to claim 1 a kind of based on ultrasonics and the bimodal of electromagnetism excitation cell system, feature exists
In: it is described that cell system is motivated based on ultrasonics and the bimodal of electromagnetism, including low-frequency pulse ultrasonic action system and low
Frequency electromagnetic field excitation system two parts, middle-low frequency pulse ultrasonic action system are put by power module, signal generating module, driving
Big module, ultrasonic action module and detection module composition, low frequency electromagnetic field excitation system is by power module, magnetic pumping module group
At.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910332267.3A CN110051939A (en) | 2019-04-22 | 2019-04-22 | It is a kind of to motivate cell system based on ultrasonics and the bimodal of electromagnetism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910332267.3A CN110051939A (en) | 2019-04-22 | 2019-04-22 | It is a kind of to motivate cell system based on ultrasonics and the bimodal of electromagnetism |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110051939A true CN110051939A (en) | 2019-07-26 |
Family
ID=67320397
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910332267.3A Pending CN110051939A (en) | 2019-04-22 | 2019-04-22 | It is a kind of to motivate cell system based on ultrasonics and the bimodal of electromagnetism |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110051939A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110577896A (en) * | 2019-10-25 | 2019-12-17 | 上海市同济医院 | Ultrasonic stimulation device and method capable of promoting proliferation and differentiation of spinal cord-derived neural stem cells |
| CN110824199A (en) * | 2019-11-01 | 2020-02-21 | 西安交通大学 | Ultrasonic emission adjusting device and method for USICM |
| CN113101526A (en) * | 2021-04-12 | 2021-07-13 | 中国医学科学院生物医学工程研究所 | Noninvasive deep brain precise composite field stimulation device based on magnetoacoustic coupling effect |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1784184A (en) * | 2003-04-10 | 2006-06-07 | 光生物科学有限责任公司 | Photomodulation methods and devices for regulating cell proliferation and gene expression |
| CN101237904A (en) * | 2005-06-03 | 2008-08-06 | 康奥尼斯有限公司 | Methods for modulating chondrocyte proliferation using pulsing electromagnetic fields |
| US20130288260A1 (en) * | 2010-10-15 | 2013-10-31 | The Research Foundation Of State University Of New York | Compositions and Methods For Enhancing the Biological Response to Chemical Agents and Physical Stimuli |
-
2019
- 2019-04-22 CN CN201910332267.3A patent/CN110051939A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1784184A (en) * | 2003-04-10 | 2006-06-07 | 光生物科学有限责任公司 | Photomodulation methods and devices for regulating cell proliferation and gene expression |
| CN101237904A (en) * | 2005-06-03 | 2008-08-06 | 康奥尼斯有限公司 | Methods for modulating chondrocyte proliferation using pulsing electromagnetic fields |
| US20130288260A1 (en) * | 2010-10-15 | 2013-10-31 | The Research Foundation Of State University Of New York | Compositions and Methods For Enhancing the Biological Response to Chemical Agents and Physical Stimuli |
Non-Patent Citations (3)
| Title |
|---|
| LI-YI SUN等: "《Effect of Pulsed Electromagnetic Field on the Proliferation and Differentiation Potential of Human Bone Marrow Mesenchymal Stem Cells》", 《BIO ELECTRO MAGNETICS》 * |
| YUN-KYONG CHOI等: "《combined effect of pulsed electromagnetic field and sound wave on in vitro and in vivo neural differentiation of human mesenchymal stem cells》", 《BIOTECHNOLOGY PROGRESS》 * |
| 杨秀芷: "《基于低强度超声激励的细胞活性提升技术研究》", 《中国优秀硕士学位论文全文数据库基础科学辑》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110577896A (en) * | 2019-10-25 | 2019-12-17 | 上海市同济医院 | Ultrasonic stimulation device and method capable of promoting proliferation and differentiation of spinal cord-derived neural stem cells |
| CN110824199A (en) * | 2019-11-01 | 2020-02-21 | 西安交通大学 | Ultrasonic emission adjusting device and method for USICM |
| CN113101526A (en) * | 2021-04-12 | 2021-07-13 | 中国医学科学院生物医学工程研究所 | Noninvasive deep brain precise composite field stimulation device based on magnetoacoustic coupling effect |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110051939A (en) | It is a kind of to motivate cell system based on ultrasonics and the bimodal of electromagnetism | |
| US9493765B2 (en) | Compact subnanosecond high voltage pulse generation system for cell electro-manipulation | |
| Xie et al. | Low‐intensity pulsed ultrasound promotes the proliferation of human bone mesenchymal stem cells by activating PI3K/AKt signaling pathways | |
| CN109124759A (en) | Cooperate with generation method, device, medium and the electronic equipment of electric impulse signal | |
| Denaro et al. | Effect of pulsed electromagnetic fields on human tenocyte cultures from supraspinatus and quadriceps tendons | |
| Nucci et al. | Stem cells labeled with superparamagnetic iron oxide nanoparticles in a preclinical model of cerebral ischemia: a systematic review with meta-analysis | |
| Li et al. | Optimum intensities of ultrasound for PGE2 secretion and growth of osteoblasts | |
| Nurković et al. | Combined effects of electromagnetic field and low-level laser increase proliferation and alter the morphology of human adipose tissue-derived mesenchymal stem cells | |
| Sand et al. | Electrical and mechanical stimulation of hair cells in the mudpuppy | |
| Zhao et al. | Applications of ultrasound to enhance mycophenolic acid production | |
| Fiáth et al. | Large-scale recording of thalamocortical circuits: in vivo electrophysiology with the two-dimensional electronic depth control silicon probe | |
| CN108865881A (en) | Cell function regulator control system and regulation method based on sound magnetic coupling electro photoluminescence principle | |
| CN107669270A (en) | A kind of magnetosonic Electrical imaging method and apparatus of electromagnetic focusing ultrasonic action | |
| Alberto et al. | Electromagnetic information delivery as a new tool in translational medicine | |
| CN105251141A (en) | Closed-loop transcranial magnetoacoustic stimulation device | |
| US11318304B2 (en) | System and method for electroporation controlled by electrical impedance measurements | |
| WO2020011130A1 (en) | Combination wave-based therapeutic apparatus and system | |
| Bohari et al. | Pulsed-low intensity ultrasound enhances extracellular matrix production by fibroblasts encapsulated in alginate | |
| CN104548392B (en) | TCD,transcranial Doppler stimulating apparatus and stimulating method | |
| Jiang et al. | Nerve defect repair by differentiated adipose-derived stem cells and chondroitinase ABC-treated acellular nerves | |
| Li et al. | Cell activity modulation and its specific function maintenance by bioinspired electromechanical nanogenerator | |
| CN110577896A (en) | Ultrasonic stimulation device and method capable of promoting proliferation and differentiation of spinal cord-derived neural stem cells | |
| Bohari et al. | Pulsed low-intensity ultrasound increases proliferation and extracelluar matrix production by human dermal fibroblasts in three-dimensional culture | |
| Lee et al. | The osteogenic differentiation of human dental pulp stem cells through G0/G1 arrest and the p-ERK/Runx-2 pathway by sonic vibration | |
| CN203874283U (en) | Multi-waveform electromagnetic field generator based on HelmHolz coil |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190726 |
|
| WD01 | Invention patent application deemed withdrawn after publication |