CN110824199A

CN110824199A - Ultrasonic emission adjusting device and method for USICM

Info

Publication number: CN110824199A
Application number: CN201911061104.2A
Authority: CN
Inventors: 庄健; 郑强强; 程磊; 郭俊康; 贾康; 洪军
Original assignee: Xian Jiaotong University
Current assignee: Xian Jiaotong University
Priority date: 2019-11-01
Filing date: 2019-11-01
Publication date: 2020-02-21
Anticipated expiration: 2039-11-01
Also published as: CN110824199B

Abstract

The ultrasonic emission adjusting device and method for the USICM are light in weight and good in dynamic characteristics. The device comprises an ultrasonic transmitting module and a sample culture dish module; the ultrasonic transmitting module comprises a bottom plate, an ultrasonic transmitting head fixed on the bottom plate and an acoustic lens arranged on the ultrasonic transmitting head; the sample culture dish module comprises a spiral ring, a culture dish, a left side plate and a right side plate; the left side plate and the right side plate are both arranged in an arc shape and symmetrically fixed on the bottom plate, and the inner side of the arc shape is provided with threads; the screw ring is in threaded fit with the threads of the left side plate and the right side plate together; the culture dish is fixed on the spiral ring, and a round hole is arranged at the center of the culture dish. According to the method, a prepared sample is sealed and fixed above a circular hole of a culture dish, then the culture dish with a spiral ring is screwed on a left side plate and a right side plate, the upper and lower heights of the culture dish are adjusted according to the inherent characteristics of an ultrasonic emission head and an acoustic lens and the specific requirements of an experiment, and the USICM ultrasonic emission adjustment is completed.

Description

Ultrasonic emission adjusting device and method for USICM

Technical Field

The invention relates to the field of scanning probe microscopes, in particular to an ultrasonic emission adjusting device and method for USICM.

Background

A Scanning Ion Conductance Microscope (SICM) is a Scanning Probe Microscope (SPM) developed gradually in recent years, which acquires surface topography information and structural characteristics of a sample by collecting current signals in an ultra-micro glass tube probe. The scanning ion conductance microscope has no contact with the sample in the scanning process, has no damage to the sample (such as living cells), and is a non-contact scanning probe microscope. And the method can carry out nano-scale imaging on the three-dimensional surface morphology of the sample to be detected under physiological conditions, and is particularly suitable for researching living cells under physiological conditions. In addition, the ion conductance microscope has the advantages of no requirement on the conductivity of a tested sample, simple sample preparation, high imaging resolution and the like, so that the ion conductance microscope is widely applied to the fields of medicine, life science, nano processing and the like in recent years. However, SICM can only acquire topographical information or structural characteristics of a sample surface, and cannot image structures inside cells, so that the inside of cells cannot be studied more deeply.

Fluorescence microscopy (fluoroscope microscopy) is currently the main device for observing the internal structure of cells. Some substances in cells, such as chlorophyll and the like, can fluoresce after being irradiated by ultraviolet rays; some other substances can not fluoresce, but can also fluoresce after being dyed by fluorescent dye or fluorescent antibody and being irradiated by ultraviolet ray, and through development for many years, people have developed various fluorescent dyes or fluorescent antibodies for different organelles, so that the fluorescent microscope has been widely applied to the field of life science and can carry out positioning qualitative observation and analysis on the structure, the shape, the protein expression, the subcellular structure and the like of tissues and cells. However, the fluorescence microscope can only observe one fluorescence at a time, and cannot simultaneously observe the structure of the whole cell, multiple observations are required to observe a plurality of fluorescence-labeled samples, the samples are damaged greatly, and some fluorescent dyes or fluorescent antibodies have cytotoxicity or may change the original properties of the cells. In addition, the fluorescence microscope can only observe under a two-dimensional environment, and the fluorescence scattering light is too strong, so that the actual resolution is greatly reduced. Although the laser confocal scanning microscope (LSCM) has high resolution, three-dimensional shape measurement can be realized, multiple laser channels can be scanned simultaneously, and multiple channels can be detected simultaneously, but the sample also needs fluorescent marks, is complicated to manufacture, and changes the original state of cells. While Electron Microscopy (EM) requires use under vacuum conditions, it is clearly not suitable for imaging living cells.

Ultrasonic waves have high frequency, the wavelength of the ultrasonic waves is much shorter than that of sound waves, diffraction phenomena are not obvious, directional and concentrated beams can be easily obtained, the beams can be propagated along straight lines within a certain distance, partial reflection can occur when the beams meet obstacles or on a medium interface, and the reflection and refraction laws are followed. The characteristic of Ultrasonic waves is utilized, the SICM platform is transformed on the existing SICM platform, an Ultrasonic emission source is added below a sample, external excitation is applied to the sample in the process of scanning the sample, the difference of the internal structure of the sample can affect the propagation of the Ultrasonic waves, and further different changes of the surface topography of the sample are caused, the internal structure of the sample can be detected through the changes, and the basic principle of an Ultrasonic Scanning Ion Conductance Microscope (USICM) is the basic principle of the USICM. In theory, three-dimensional imaging of all structures within a cell under physiological conditions can be achieved using the USICM, and therefore, in order to achieve the above experimental objectives, an ultrasound emission modulation device for the USICM platform is required.

Disclosure of Invention

Aiming at the problems in the prior art, the invention provides an ultrasonic emission adjusting device and method for USICM, which have the advantages of compact structure, light weight and good dynamic characteristics.

The invention is realized by the following technical scheme:

an ultrasonic emission adjustment device for USICM comprises an ultrasonic emission module and a sample culture dish module;

the ultrasonic transmitting module comprises a bottom plate, an ultrasonic transmitting head fixed on the bottom plate and an acoustic lens arranged on the ultrasonic transmitting head;

the sample culture dish module comprises a spiral ring, a culture dish, a left side plate and a right side plate; the left side plate and the right side plate are both arranged in an arc shape and symmetrically fixed on the bottom plate, and the inner side of the arc shape is provided with threads; the screw ring is in threaded fit with the threads of the left side plate and the right side plate together; the culture dish is fixed on the spiral ring, and a round hole is formed in the center of the culture dish;

the helicoidal, the culture dish, the acoustic lens and the ultrasonic transmitting head are arranged along the same axis.

Preferably, the ultrasonic transmitting module further comprises an ultrasonic transmitting head fastening nut; the base plate is provided with a circular bulge with a thread on the outer wall, and the circular bulge is matched with the internal thread of the fastening nut of the ultrasonic emitter to clamp the ultrasonic emitter; the center of the fastening nut of the ultrasonic transmitting head is provided with a through hole for penetrating through the lug boss of the ultrasonic transmitting head.

Furthermore, the ultrasonic transmitting module also comprises an ultrasonic transmitting head wire; an electrode is arranged at the bottom of the ultrasonic transmitting head, a groove is arranged below the annular protrusion on the bottom plate, and the electrode of the ultrasonic transmitting head is led out from the groove; the ultrasonic transmitting head wire is inserted in the groove and connected with the electrode of the ultrasonic transmitting head.

Preferably, the left side plate or the right side plate is provided with scales, and the scales 0 and the upper surface of the ultrasonic transmitting head are on the same horizontal plane.

Preferably, the culture dish is nested and fixed in the inner circle of the coil, and the lower surface of the coil and the upper surface of the bottom of the culture dish are in the same horizontal plane.

Preferably, a groove is formed in the side wall of the culture dish, an Ag/AgCl electrode plug is fixedly arranged in the groove, and an Ag/AgCl electrode lead is inserted into the Ag/AgCl electrode plug; an Ag/AgCl electrode which is electrically connected is fixed on the Ag/AgCl electrode plug.

Preferably, the acoustic lens is cone-shaped, the small end is attached to the center of the upper surface of the ultrasonic emitting head, and the large end is arranged towards the culture dish.

Preferably, the left side plate and the right side plate are fixedly connected to the bottom plate through bolts respectively.

Preferably, the device further comprises a supporting plate; one end of the supporting plate is provided with a piezoelectric ceramic connecting countersunk hole, and the other end of the supporting plate is fixedly connected with the bottom plate.

An ultrasonic wave emission adjusting method for USICM, based on the adjusting device in any one of the above items; sealing and bonding the prepared sample above the circular hole of the culture dish, screwing the culture dish with a screw ring on the left side plate and the right side plate, and adjusting the vertical height of the culture dish according to the inherent characteristics of the ultrasonic emission head and the acoustic lens and the specific requirements of the experiment; and after the adjustment is finished, inserting an Ag/AgCl electrode wire to finish the USICM ultrasonic emission adjustment.

Compared with the prior art, the invention has the following beneficial technical effects:

the ultrasonic emission adjusting device for USICM fixes the ultrasonic emission head, and then forms an adjusting device capable of moving up and down through the matching of the left side plate, the right side plate and the helicoidal ring, so that the culture dish can coaxially move and adjust relative to the ultrasonic emission, the distance between the bottom of the sample and the ultrasonic emission head can be adjusted by adjusting the height of the culture dish according to different characteristics or actual experimental requirements of the ultrasonic emission head, the adjustment of ultrasonic emission is realized, the focusing of the arranged acoustic lens is utilized to ensure the action effect of ultrasonic waves, the ultrasonic emission adjusting device can directly act on the sample by matching with the arranged round hole, and the ultrasonic loss and interference formed by multiple times of passing through a medium interface are avoided.

Furthermore, the inner circle of the coil is fixed with the culture dish, the lower surface of the coil and the upper surface of the bottom of the culture dish are in the same horizontal plane, and the distance between the bottom of the sample and the ultrasonic emission head can be conveniently adjusted by using the so-called reference surface of the lower surface of the coil during adjustment. And meanwhile, the scale is matched with the scale, so that the distance between the bottom of the sample and the upper surface of the ultrasonic transmitting head can be conveniently obtained.

Furthermore, in the prior SICM platform experiment, Ag/AgCl electrodes are freely placed in a solution, and the Ag/AgCl electrodes are easily separated from the solution due to sample local movement in the scanning process, so that a working circuit is disconnected, and the experiment fails. The ultrasonic emission adjusting device for the USICM welds the Ag/AgCl electrode and the wiring terminal together and is fixed on the side wall of a culture dish, only another wiring terminal needs to be inserted on the wiring terminal during an experiment, scanning imaging can be carried out, and the stability and the reliability are greatly improved.

Drawings

FIG. 1 is an isometric view of the overall construction of the device in an example of the invention;

FIG. 2 is a schematic view in another perspective of the overall construction of the device in an example of the invention;

FIG. 3 is a view showing a structure of a culture dish holding structure of the apparatus according to the embodiment of the present invention;

FIG. 4 is a bottom view of FIG. 3;

FIG. 5 is an exploded view of the culture dish module of the apparatus in an example of the invention;

FIG. 6 is an exploded view of the ultrasonic transmitter module of the device in an example of the invention;

in the figure, 1 is a bottom plate, 2 is an ultrasonic emitter, 3 is an ultrasonic emitter fastening nut, 4 is a spiral ring, 5 is a culture dish, 6 is a left side plate, 7 is a right side plate, 8 is an Ag/AgCl electrode plug, 9 is an Ag/AgCl electrode wire, 10 is a first bolt, 11 is a second bolt, 12 is a sample, 13 is an acoustic lens, 14 is a supporting plate, 15 is an ultrasonic emitter wire, 16 is a piezoelectric ceramic connecting countersunk hole, 17 is a bottom plate connecting countersunk hole, 18 is an ultrasonic emitter anode, 19 is an ultrasonic emitter cathode, 20 is a round hole, and 21 is an Ag/AgCl electrode.

Detailed Description

The present invention will now be described in further detail with reference to specific examples, which are intended to be illustrative, but not limiting, of the invention.

The invention relates to an ultrasonic emission adjusting device for USICM, which mainly comprises an ultrasonic emission head, an acoustic lens, a bottom plate, a wave emission head fastening nut, a culture dish, a spiral ring, a left side plate, a right side plate, an Ag/AgCl electrode plug and the like. The device can apply an external excitation to the sample when the sample is scanned, and further push out the structural characteristics inside the sample through the change of the surface topography of the sample, thereby realizing the simultaneous three-dimensional imaging of all structures inside the cell under physiological conditions. The distance between the sample and the ultrasonic transmitting head can be adjusted according to actual needs. Ag/AgCl electrodes are fixed on the side wall of the culture dish to improve the stability and reliability of the experiment.

The invention relates to an ultrasonic emission adjusting device for USICM, which comprises an ultrasonic emission module and a sample culture dish module, wherein the whole device is fixedly connected to piezoelectric ceramics through a supporting plate 14; one end of the supporting plate 14 is provided with a counter bore 16 for connecting to the piezoelectric ceramics; the other end of the supporting plate 14 is provided with a threaded hole and is connected with the bottom plate 1 through a first bolt 10, and in the preferred embodiment, the first bolt 10 is an M3 hexagon socket head cap bolt.

The ultrasonic transmitting module comprises an ultrasonic transmitting head 2, a bottom plate 1 for positioning the ultrasonic transmitting head, an ultrasonic transmitting head fastening nut 3 for clamping the ultrasonic transmitting head, an acoustic lens 13 for converging ultrasonic waves and an ultrasonic transmitting head lead 15; the acoustic lens 13 is formed in a tapered shape and fixed to the center of the upper surface of the ultrasonic transmitter head 2.

The sample culture dish module comprises a sample 12, a culture dish 5 for placing the sample, a left side plate 6 and a right side plate 7 for fixing the culture dish, a spiral ring 4 which is fixed with the culture dish and enables the height of the culture dish to be adjusted up and down, an Ag/AgCl electrode plug 8 and an Ag/AgCl electrode lead 9. The left end and the right end of the bottom plate 1 are provided with countersunk holes which are respectively connected with the left side plate 6 and the right side plate 7 through second bolts 11; the left side plate 6 and the right side plate 7 are both arc-shaped plates, threads are arranged on the inner sides of the arc-shaped plates, and the threads of the left side plate and the right side plate are matched with the helicoidal ring 4 together. The front surface of the right side plate 7 is provided with scales, and the scale 0 and the upper surface of the ultrasonic transmitting head 2 are on the same horizontal plane. The inner circle of the coil 4 is fixed with the culture dish 5, the distance between the lower surface of the coil 4 and the lower surface of the culture dish 5 is 1mm, and the lower surface and the upper surface of the bottom of the culture dish 5 are in the same horizontal plane, and in the preferred embodiment, the second bolt 11 is an M2 hexagon socket head cap screw.

The culture dish 5 has a circular hole 20 at the center for transmitting the ultrasonic waves, and the specimen 12 is adhered above the circular hole 20, and the ultrasonic waves are applied to the specimen 12 through the circular hole 20. The side wall of the culture dish 5 is provided with a groove, an Ag/AgCl electrode plug 8 containing an Ag/AgCl electrode 21 is fixed in the groove, and an Ag/AgCl electrode lead 9 is inserted in the Ag/AgCl electrode plug 8.

The center of the bottom plate 1 is provided with an annular bulge for fixing the ultrasonic emitter 2, and the outer wall of the annular bulge is tapped with threads which are matched with the internal threads of the fastening nut 3 of the ultrasonic emitter to clamp the ultrasonic emitter 2; the ultrasonic transmitter head fastening nut 3 has a through hole at the center thereof for penetrating the boss of the ultrasonic transmitter head 2. A groove is arranged in the middle of the bottom plate 1, the electrode of the ultrasonic transmitting head 2 is led out from the groove, the longer electrode 18 is the anode, and the shorter electrode 19 is the cathode; the ultrasonic transmitting head lead 15 is inserted in the groove and connected with the electrode of the ultrasonic transmitting head 2.

Specifically, as shown in FIG. 1, the present invention provides an ultrasound emission modulation device for USICM, comprising an ultrasound emission module and a sample petri dish module, and a fascia 14 fixedly connecting the entire device to a piezoelectric ceramic.

The main components of the ultrasonic transmitting module are an ultrasonic transmitting head 2 and an ultrasonic transmitting head fastening screw cap 3; the sample culture dish module mainly comprises a left side plate 6, a right side plate 7, a spiral ring 4 and a culture dish 5. The left end of the supporting plate 14 is provided with four piezoelectric ceramic connecting counter bores 16 for fixing the supporting plate 14 on the piezoelectric ceramic. The right end of the supporting plate 14 is provided with four threaded holes M3, correspondingly, the bottom plate 1 is provided with four bottom plate connecting counter bores 17, and the bottom plate 1 and the supporting plate 14 are connected together through the first bolts 10, as shown in figure 2, so that the piezoelectric ceramic supporting plate has good dynamic characteristics.

As shown in FIG. 3, the structure of the fixed culture dish mainly comprises a culture dish 5, a left side plate 6, a right side plate 7, a bottom plate 1 and a coil 4. Four side plates are arranged at four corners of the bottom plate 1 to be connected with countersunk holes, the left side plate 6 and the right side plate 7 are correspondingly provided with two threaded holes respectively, and the left side plate 6 and the right side plate 7 are connected together through second bolts 11, as shown in fig. 4. Left side board 6 and right side board 7 are the arc, and the inboard is equipped with the screw thread, and the screw thread of controlling the curb plate cooperates with helicoidal 4 jointly, and helicoidal 4 can be adjusted from top to bottom, and then drives culture dish and sample and reciprocate.

As shown in FIG. 5, the sample petri dish module comprises a sample 12, an Ag/AgCl electrode plug 8 and an Ag/AgCl electrode lead 9 in addition to the petri dish 5, the left side plate 6, the right side plate 7, the bottom plate 1 and the coil 4. The center of culture dish has a round hole 20, and the sample bonds in round hole 20 top, plays sealed effect when fixed, and the ultrasonic wave can direct action on the sample through round hole 20, avoids the ultrasonic loss and the interference of passing through the formation of medium interface many times. In addition, the inner circle of the coil is fixed with the culture dish, so the heights of the sample and the culture dish can be adjusted by adjusting the height of the coil. The thickness of the bottom plate of the culture dish is 1mm, so the distance between the lower surface of the spiral ring and the lower surface of the culture dish is 1mm, and the distance and the upper surface of the bottom of the culture dish are in the same horizontal plane, so that the distance between the bottom of the sample and the ultrasonic transmitting head can be conveniently adjusted by using the so-called reference surface of the lower surface of the spiral ring during adjustment. The front surface of right side board is equipped with the scale, and 0 scale and ultrasonic emission head's upper surface are on same horizontal plane, can be used for the scale of sample bottom and ultrasonic emission head upper surface distance. In addition, in order to avoid the separation of the Ag/AgCl electrode from the solution in the scanning process, a groove is formed in the side wall of the culture dish, an Ag/AgCl electrode plug containing the Ag/AgCl electrode is directly fixed in the groove, then an Ag/AgCl electrode lead is inserted into the Ag/AgCl electrode plug, and during the experiment, the electrode is always kept to float freely in the solution of the culture dish.

As shown in FIG. 6, the ultrasonic transmitter module comprises an ultrasonic transmitter head 2, a base plate 1 for positioning the ultrasonic transmitter head, an ultrasonic transmitter head fastening nut 3 for clamping the ultrasonic transmitter head, and an acoustic lens 13 for converging ultrasonic waves and an ultrasonic transmitter head wire 15. The center of the bottom plate 1 is provided with a circular ring-shaped bulge for fixing the ultrasonic emitter 2, the outer wall of the circular ring-shaped bulge is tapped with threads, and the circular ring-shaped bulge is matched with the internal threads of the fastening nut 3 of the ultrasonic emitter to clamp the ultrasonic emitter 2. A through hole is formed at the center of the ultrasonic transmitter head fastening nut 3 to penetrate through the boss of the ultrasonic transmitter head 2. The middle of the bottom plate 1 is provided with a groove, the electrode of the ultrasonic transmitting head 2 is led out from the groove, the longer electrode 18 is the anode, the shorter electrode 19 is the cathode, and the ultrasonic transmitting head lead 15 is inserted into the groove and connected with the electrode of the ultrasonic transmitting head 2. The acoustic lens 13 is tapered and fixed to the center of the upper surface of the ultrasonic transmitter head 2, and focuses the ultrasonic waves transmitted from the ultrasonic transmitter head to one point.

The typical process of carrying out the experiment by using the USICM scanning experiment platform provided with the invention is as follows:

when the USICM scanning experiment is carried out by using the ultrasonic emission adjusting device, the ultrasonic emission adjusting method for the USICM is realized by firstly respectively installing the left side plate 6 and the right side plate 7 on the bottom plate 1 by using the second bolt 11, then fixing the bottom plate 1 on the supporting plate 14 by using the first bolt 10, and after the installation is finished, integrally installing the supporting plate 14, the bottom plate 1, the left side plate 6 and the right side plate 7 on the piezoelectric ceramics through the countersunk holes 16 on the supporting plate. Install ultrasonic emission head 2 in the ring channel on bottom plate 1, the electrode is drawn forth along the rectangular groove of bottom plate, then screws up ultrasonic emission head fastening nut 3 and fixes ultrasonic emission head 2, fixes acoustic lens 13 at the upper surface central authorities of emission head 2, inserts ultrasonic emission head wire 15 in the rectangular groove of bottom plate 1 again, is connected with ultrasonic emission head 2's electrode. Then, the coil 4 and the culture dish 5 are fastened together, the distance between the lower surface of the coil 4 and the lower surface of the culture dish 5 is 1mm, and then the Ag/AgCl electrode plug 8 welded with the Ag/AgCl electrode is fixed in the groove on the side wall of the culture dish. To this end, the ultrasonic emission modulation device for USICM is substantially installed.

Subsequently, the prepared specimen 12 is stuck on the top of the culture dish 5, taking care that the seal is well fixed to prevent leakage of the solution or movement of the specimen, and then the culture dish 5 with the coil 4 is screwed on the left and

right side plates

6 and 7, and the up and down height of the culture dish 5 is adjusted according to the inherent characteristics of the ultrasonic wave emitting head 2 and the acoustic lens 13 and the specific needs of the experiment. After the adjustment is completed, the Ag/AgCl electrode lead 9 is inserted into the Ag/AgCl electrode plug 8. And finally, completing the connection adjustment work of other parts of the USICM experiment platform, adjusting relevant parameters, and starting the scanning experiment of the USICM experiment platform.

According to the ultrasonic emission adjusting device for the USICM, the sample, the culture dish, the ultrasonic emission head and the Ag/AgCl electrode are stably and reliably mounted and fixed, and can be used according to different characteristics of the ultrasonic emission head or actual experiment requirements; compared with the traditional scanning ion conductance microscope, the ultrasonic scanning ion conductance microscope adds ultrasonic wave excitation below a sample, and the internal structure of the sample is deduced by detecting the change condition of the surface of the sample under the excitation action. The ultrasonic ion conductance microscope solves the problems that a sample needs to be dyed, all organelles cannot be imaged simultaneously, the resolution ratio is too low or imaging cannot be carried out under the physiological condition of the cell in the existing intracellular imaging technology, and the like, can realize simultaneous three-dimensional imaging of all structures inside the cell under the physiological condition, does not need dyeing or other pretreatment on the sample, greatly simplifies the experimental steps, and provides a new method for subcellular structure imaging of the cell.

Claims

1. An ultrasonic emission adjustment device for USICM is characterized by comprising an ultrasonic emission module and a sample culture dish module;

the ultrasonic transmitting module comprises a bottom plate (1), an ultrasonic transmitting head (2) fixed on the bottom plate (1) and an acoustic lens (13) arranged on the ultrasonic transmitting head (2);

the sample culture dish module comprises a spiral ring (4), a culture dish (5), a left side plate (6) and a right side plate (7); the left side plate (6) and the right side plate (7) are both arranged in an arc shape and symmetrically fixed on the bottom plate (1), and the inner side of the arc shape is provided with threads; the helicoids (4) are in threaded fit with the threads of the left and right side plates together; the culture dish (5) is fixed on the spiral ring (4), and a round hole (20) is formed in the center of the culture dish (5);

the helicoidal (4), the culture dish (5), the acoustic lens (13) and the ultrasonic transmitting head (2) are arranged along the same axis.

2. An ultrasonic emission adjustment device for USICM according to claim 1, characterized in that the ultrasonic emission module further comprises an ultrasonic emission head fastening nut (3); the bottom plate (1) is provided with a circular bulge with a threaded outer wall, and the circular bulge is matched with the internal thread of the ultrasonic emitter fastening nut (3) to clamp the ultrasonic emitter (2); the center of the ultrasonic transmitting head fastening nut (3) is provided with a through hole for penetrating through the lug boss of the ultrasonic transmitting head (2).

3. An ultrasound transmission adjustment device for use in a USICM according to claim 2, wherein said ultrasound transmission module further comprises an ultrasound transmission head wire (15); an electrode is arranged at the bottom of the ultrasonic transmitting head (2), a groove is arranged below the circular-ring-shaped protrusion on the bottom plate (1), and the electrode of the ultrasonic transmitting head (2) is led out from the groove; the ultrasonic transmitting head lead (15) is inserted in the groove and connected with the electrode of the ultrasonic transmitting head (2).

4. An ultrasonic emission control device for USICM according to claim 1, characterized in that the left side plate (6) or the right side plate (7) is provided with a scale, 0 scale is on the same level with the upper surface of the ultrasonic emission head (2).

5. An ultrasound transmission adjustment device for USICM according to claim 1, characterized in that the culture dish (5) is nested and fixed in the inner circle of the coil (4), the lower surface of the coil (4) being in the same level with the upper surface of the bottom of the culture dish (5).

6. An ultrasonic emission control device for USICM according to claim 1 or 5, characterized in that the side wall of the culture dish (5) is provided with a groove, an Ag/AgCl electrode plug (8) is fixedly arranged in the groove, and an Ag/AgCl electrode lead (9) is inserted in the Ag/AgCl electrode plug (8); an Ag/AgCl electrode (21) which is electrically connected is fixed on the Ag/AgCl electrode plug (8).

7. An ultrasound transmission adjustment device for USICM according to claim 1, characterized in that the acoustic lens (13) is cone-shaped, with the small end fitting in the center of the upper surface of the ultrasound transmission head (2) and the large end facing the culture dish.

8. An ultrasonic emission control device for USICM according to claim 1, characterized in that the left side plate (6) and the right side plate (7) are fixedly connected to the base plate (1) by bolts, respectively.

9. An ultrasonic emission regulation device for use in a USICM according to claim 1, further comprising a support plate (14); one end of the supporting plate (14) is provided with a piezoelectric ceramic connecting countersunk hole (16), and the other end is fixedly connected with the bottom plate (1).

10. An ultrasonic emission regulation method for USICM, characterized in that it is based on the regulation device of any one of claims 1-9; the prepared sample (12) is sealed, bonded and fixed above a round hole (20) of a culture dish (5), then the culture dish (5) with a spiral ring (4) is screwed on a left side plate (6) and a right side plate (7), and the vertical height of the culture dish (5) is adjusted according to the inherent characteristics of the ultrasonic transmitting head (2) and the acoustic lens (13) and the specific requirements of the experiment; and after the adjustment is finished, an Ag/AgCl electrode lead (9) is inserted to finish the USICM ultrasonic emission adjustment.