CN110051695A - A kind of drug and preparation method thereof preventing senile dementia - Google Patents
A kind of drug and preparation method thereof preventing senile dementia Download PDFInfo
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- CN110051695A CN110051695A CN201910115686.1A CN201910115686A CN110051695A CN 110051695 A CN110051695 A CN 110051695A CN 201910115686 A CN201910115686 A CN 201910115686A CN 110051695 A CN110051695 A CN 110051695A
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Abstract
The present invention relates to pharmaceutical technology fields, and in particular to a kind of drug and preparation method thereof for preventing senile dementia.A kind of drug and preparation method thereof preventing senile dementia, comprising the following steps: A, crushing；B, ethyl alcohol impregnates；C, refluxing extraction；D, it is concentrated under reduced pressure, obtains American cockroach ethyl alcohol concentrate；E, grease is layered；F, it is concentrated under reduced pressure, obtains American-cockroach-extract medicinal extract；G, American-cockroach-extract medicinal extract, sugar are taken by weight, and water is uniformly mixed；H, high-temperature sterilization；I, lactobacillus inoculum, anaerobic fermentation are added；J, after-ripening；K, vacuum refrigeration is dry, crushes to get the drug of prevention senile dementia.The present invention makes to assemble the specific effective ingredient of senile dementia in American cockroach, macromolecular substances are reduced, and useful small-molecule active substance such as fatty acid, polypeptide and amino acid content increase, to improve prevention and improvement of the simple American-cockroach-extract to senile dementia.
Description
Technical field
The present invention relates to pharmaceutical technology fields, and in particular to a kind of drug and preparation method thereof for preventing senile dementia.
Background technique
The senile dementia of the namely popular upper theory of Alzheimer disease (AD) is a kind of carry out sexual development of onset concealment
Nervous system degenerative disease.Clinically with memory disorders, aphasia, appraxia, agnosia, the damage of visual space technical ability, execution function
The performance of the generalized dementias such as obstacle and personality and behavior change is characterized.People of the Alzheimer disease in over-65s age bracket
Illness rate is 5% in group, and more than 85 years old illness rate increases to 25%, and 95 years old or more old man is then up to 60%, but senile at present silly
Ideal treatment method not yet is stayed, therefore early prevention is critically important.
Many people think, with advancing age, failure of memory, it is slow in reacting be the performance of normal aging, but cure
It learns development and discloses the early symptom that failure of memory may be Alzheimer disease (senile dementia), be that brain goes wrong
Attention device, therefore when failure of memory occurs in old man in manager, to draw attention, see a doctor as early as possible.
American cockroach (Periplanetaamericana) is the maximum insect of volume in Blattidae, at polypide long 29~35
Millimeter, bronzing, wing are longer than abdomen end.Feeler is very long, there is biggish butterfly brown marking among pronotary, after speckle
Edge has complete yellow band line, originates in South America, and feeding habits are extensive, are fond of sugar and starch, contaminated food.
American cockroach is traditional Chinese medicine, is used as medicine with adult.Taste cold in nature is salty, toxic, have acid, have the scattered stasis of blood, disperse accumulation,
The functions such as removing toxic substances, Li Shui, detumescence.Modern studies have found that American cockroach epidermis contain sclerotin, chitin, bromine, zinc, nickel, it is violent,
The elements such as potassium, calcium, titanium, chlorine, sulphur, silicon, aluminium, magnesium, muscle hydrolyze 13 kinds of amino acid, and body is stored micro-element B1, B2, niacin and resisted
Bad hematic acid etc., lymph contain erythrothioneine, dragon containing trehalose, trehalase, glycoprotein, inositol, protocatechuic acid grape glucoside etc., entirety
Shrimp neosine, trigonelline, glycine, glycine betaine, anus alkali, trimethylamine, adenine etc..
American cockroach dries polypide extract, contains WHF (polyalcohols, epidermal growth factor), viscous propylhomoserin, viscous sugared propylhomoserin
And a variety of amino acid isoreactivity substances, have and cell Proliferation and new granulation tissue is promoted to increase, accelerates disease damage tissue repair, add
The effects of fast necrotic tissue falls off, improves body's immunity.
China Patent No. be 201510088479.3 patent applications disclose it is a kind of treat neurodegenerative disease include Ah
Wurz sea Mo's disease, Parkinson's disease, chorea, different type spinocerebellar ataxia, amyotrophic lateral sclerosis, spinal cord
The American cockroach liquid medicine of a variety of diseases such as muscular atrophy, syringomyelia and myasthenia gravis.American cockroach liquid medicine of the invention,
It is made of the Materials Solvents auxiliary material of following parts by weight, 5-10 parts of American cockroach, is added to 90-95 parts of 30-50 degree white wine；Or
It 3-10 parts of American cockroach, is added to 5 degree of -18 degree rice wine or 90-95 parts of yellow rice wine, impregnates 20-30 days, filter respectively to obtain the final product；Such as use
Rice wine, which is soaked, need to decoct 15 minutes, filter after cooling, and sweet-scented osmanthus sauce is equipped with after removing slag or honey is a small amount of, the same day drinks；With yellow rice wine and
The American cockroach wine that white wine is soaked can be stored the long period, if sterilizing after bottling through pasteurization or co-60 radiation, can store
It is more than half a year；Often taking American cockroach liquid medicine of the invention has prevention effect to alzheimer's disease.
American cockroach liquid medicine made from the patent is not suitable for all groups, such as in neurodegenerative disease patient, exists
There is taboo person to alcohol, the liquid medicine cannot be taken；Specially treated is not carried out to American cockroach in the liquid medicine manufacturing process, only
It is that American cockroach adds wine is brewed to form, without removing the peculiar smell of American cockroach well, mouthfeel is also bad, will have a direct impact on patient
Compliance, under the premise of keeping American cockroach original curative effect, by the processing certain to American cockroach, so as to improve mouth
Sense, is very necessary；In addition, the liquid medicine is liquid, it has not been convenient to carry, and storage time is too short.
Summary of the invention
It is an object of the invention to overcome the deficiency of the above-mentioned prior art, provide a kind of drug for preventing senile dementia and its
Preparation method, this method make to assemble the specific effective ingredient of senile dementia in American cockroach, and macromolecular substances are reduced, and useful
Small-molecule active substance such as fatty acid, polypeptide and amino acid content increase, to improve simple American-cockroach-extract pair
The prevention and improvement of senile dementia.
The present invention is attained in that using following technology
A kind of drug and preparation method thereof preventing senile dementia, comprising the following steps:
A, dry American cockroach polypide is crushed, crosses 10 meshes, American cockroach coarse powder is made；
B, the ethyl alcohol of American cockroach coarse powder concentration 90%~95% obtained by step A is impregnated, ethyl alcohol liquid level is made to be higher by coarse powder liquid level 5
American cockroach coarse powder mixed liquor is made in~10cm；
C, by the investment extractor of American cockroach coarse powder mixed liquor obtained by step B, 70~85 DEG C at a temperature of carry out normal pressure 3~
5 refluxing extractions, inventory, which is less than, extracts the 2/3 of tank volume, carries out first time extraction to American cockroach coarse powder mixed liquor, adds beauty
The ethyl alcohol of 5~8 times of the big Lian coarse powder in continent amount concentration 90%~95%, extraction time are 4~6 hours；It extracts and adds every time after second
The ethyl alcohol of 3~6 times of American cockroach coarse powder amount concentration 90%~95%, extraction time are 3~5 hours；Combined extract filters beautiful
The big Lian ethanol extract in continent；
D, American cockroach ethanol extract obtained by step C is put into single-effect concentrator, in vacuum degree -0.05~-0.06Mpa, temperature
It is concentrated under reduced pressure under conditions of 50~60 DEG C of degree, until obtaining American cockroach ethyl alcohol concentrate when specific gravity is 1.05~1.15；
E, American cockroach ethyl alcohol concentrate obtained by step D is moved into degreasing tank, addition American cockroach ethyl alcohol concentrate weight 7~
The purified water of 10 times of amounts, to constant temperature stirring 10~60 minutes again after 85~95 DEG C, mixing speed was 20~60 for warming while stirring
Rev/min, then heat preservation, which stands 1~5 hour, is layered grease, takes lower layer's aqueous；
F, lower layer's aqueous obtained by step E to be released, centrifugal filtration, filtrate moves into inspissator, at 60~80 DEG C of temperature, vacuum degree-
Be concentrated under reduced pressure under the conditions of 0.05~-0.06MPa, be concentrated into specific gravity be 1.25~1.35 when, obtain American-cockroach-extract medicinal extract；
G, American-cockroach-extract medicinal extract 1 part is taken obtained by step F by weight, and sugar 0.l~0.2 part, is stirred by 1~2 part of water
Uniformly；Filtering, filters out insoluble impurities, obtains clear mixed solution；
H, mixing liquid obtained by step G is taken to carry out high-temperature sterilization under the conditions of 100~120 DEG C of temperature, the time 10~15 minutes, so
After be cooled to 25~45 DEG C, obtain sterilized liquid；
I, sterilized liquid obtained by step H is taken, lactobacillus inoculum is then added, inoculative proportion is 0.5%~5%；Then by temperature control
System seals anaerobic fermentation 12~24 hours at 35~45 DEG C, until pH value terminates to ferment when reaching 3.8~4.5, is fermented
Liquid；
J, fermentation liquid obtained by step I is put into storage tank, is then transferred to after-ripening 12~36 hours in 0~Cool Room 4 DEG C, makes cream
Sour bacterium further degrades to polymer substances such as albumen, fat in fermentation liquid, generate required small molecule fatty acid, polypeptide with
And amino acid isoreactivity substance；
K, the fermentation liquid for terminating step J after-ripening takes out, and is put into freeze-drying disk, is transferred in vacuum freeze drier,
So that moisture is directly sublimed into gaseous state under the conditions of temperature -10~-50 DEG C, 1.3~13Pa of pressure, make material dewatering, when freeze-drying
Between about 24~48 hours, water content be 5.0%~8.0% when take out；Then 10~40 mesh are crushed to get preventing and improving old age
Dull-witted drug.
Preferably, the step C can use following technical scheme: American cockroach coarse powder mixed liquor obtained by step B is thrown
Enter in extractor, in 70~85 DEG C at a temperature of progress 3 refluxing extractions of normal pressure, inventory is less than the 2/3 of extraction tank volume, right
American cockroach coarse powder mixed liquor carries out first time extraction, adds the ethyl alcohol of 5 times of American cockroach coarse powder amount concentration 92%, extraction time 5
Hour；The ethyl alcohol of second of extraction plus 5 times of American cockroach coarse powder amount concentration 92%, extraction time are 4 hours；Third time, which is extracted, to be added
The ethyl alcohol of 5 times of American cockroach coarse powder amount concentration 92%, extraction time are 4 hours；Merge extracting solution three times, filters to obtain American cockroach
Ethanol extract.
It is highly preferred that the step E can use following technical scheme: by American cockroach ethyl alcohol concentrate obtained by step D
It moves into degreasing tank, the purified waters of 8 times of American cockroach ethyl alcohol concentrate weight amounts is added, warming while stirring is to after 85~95 DEG C
Constant temperature stirs 40 minutes again, and mixing speed is 40 revs/min, and then heat preservation, which stands 4 hours, is layered grease, takes lower layer's aqueous.
It is highly preferred that sugar is that sucrose or glucose are mixed with sucrose by weight 1:l~2 in the step F.
It is highly preferred that the lactic acid bacteria in the step I is selected from Lactobacillus casei, streptococcus thermophilus, Lactobacillus rhamnosus, plant
Object lactobacillus, lactobacillus acidophilus, lactobacillus bulgaricus, lactobacillus delbruockii subspecies bulgaricus, lactobacillus paracasei and Roy
One or more of formula lactobacillus.
It is highly preferred that lactic acid bacteria in the step I is Lactobacillus casei, streptococcus thermophilus and Lactobacillus rhamnosus, three
Kind strain inoculative proportion 1:1.8~2.2:1.8~2.2；Or lactic acid bacteria is lactobacillus acidophilus, streptococcus thermophilus and Bao Jiali
Sub- lactobacillus, strain ratio are 1:1.0~2.0:1；Or lactic acid bacteria is lactobacillus plantarum, streptococcus thermophilus and De Shi cream bar
Bacterium subspecies, strain ratio are 1:0.8~1.2:0.8~1.2.
It is highly preferred that the lactobacillus inoculum ratio in the step I is 2%~3%, fermentation temperature is controlled at 40~42 DEG C,
At 15~20 hours, fermentation ends control point pH value was 4.0~4.2 for fermentation time control.
Beneficial effects of the present invention:
1, the process route that the present invention uses can make poly- to the specific effective ingredient for preventing and improving senile dementia in American cockroach
Collection, to improve prevention and improvement of the simple American-cockroach-extract to senile dementia.
2, the present invention passes through lactobacillus-fermented, so that the peculiar peculiar smell of the insect of American-cockroach-extract is able to mitigate or disappear
It removes, and the fragrance with lactic acid.
3, the prevention that obtains of the present invention and improve the macromolecular substances that the drug of senile dementia contains and reduce, and it is useful small
Molecular activity substance such as fatty acid, polypeptide and amino acid content increase.
4, present invention preserves all nutritional ingredients and active material of American cockroach, the work of probiotics strain is also maintained
Power, and water content is low, quality is stablized, and easily stored, transport, carries sale, and long shelf-life was up to 3 years or more.
5, the present invention uses vacuum refrigeration and crushing, keeps the drug for preventing and improving senile dementia loose because of freeze-drying
It is porous, and there is instant effect, and good fluidity, instant packed and transport.
Specific embodiment
Embodiment 1
A kind of drug and preparation method thereof preventing senile dementia, comprising the following steps:
A, 200Kg dry American cockroach polypide is crushed, crosses 10 meshes, American cockroach coarse powder is made；
B, the ethyl alcohol of American cockroach coarse powder concentration 95% obtained by step A is impregnated, ethyl alcohol liquid level is made to be higher by coarse powder liquid level 5cm, made
Obtain American cockroach coarse powder mixed liquor；
C, by the investment extractor of American cockroach coarse powder mixed liquor obtained by step B, 70~85 DEG C at a temperature of carry out normal pressure 5 times
Refluxing extraction, inventory, which is less than, extracts the 2/3 of tank volume, carries out first time extraction to American cockroach coarse powder mixed liquor, adds
The ethyl alcohol of 1000Kg concentration 95%, extraction time are 4 hours；The ethyl alcohol for adding 600Kg concentration 95% is extracted after second every time, is mentioned
Taking the time is 3 hours；Combined extract filters to obtain American cockroach ethanol extract；
D, American cockroach ethanol extract obtained by step C is put into single-effect concentrator, in vacuum degree -0.05~-0.06Mpa, temperature
It is concentrated under reduced pressure under conditions of 50~60 DEG C of degree, until obtaining American cockroach ethyl alcohol concentrate when specific gravity is 1.08；
E, American cockroach ethyl alcohol concentrate obtained by step D is moved into degreasing tank, the purified water of 280Kg is added, rises while stirring
Temperature was to constant temperature stirring 60 minutes again after 85~95 DEG C, and mixing speed is 20 revs/min, and then heat preservation, which stands 1 hour, is layered grease,
Take lower layer's aqueous；
F, lower layer's aqueous obtained by step E to be released, centrifugal filtration, filtrate moves into inspissator, at 60~80 DEG C of temperature, vacuum degree-
Be concentrated under reduced pressure under the conditions of 0.05~-0.06MPa, be concentrated into specific gravity be 1.28 when, obtain American-cockroach-extract medicinal extract 25.2Kg；
G, American-cockroach-extract medicinal extract 1Kg obtained by step F is taken by weight, and sugar 0.lKg, 1 Kg of water are uniformly mixed；It crosses
Filter, filters out insoluble impurities, obtains clear mixed solution；
H, it takes mixing liquid obtained by step G to carry out high-temperature sterilization under the conditions of 100 DEG C of temperature, the time 10 minutes, is subsequently cooled to
25 DEG C, obtain sterilized liquid；
I, sterilized liquid obtained by step H is taken, lactobacillus inoculum, inoculative proportion 0.5% are then added；Then temperature is controlled
It 35~45 DEG C, seals anaerobic fermentation 12~24 hours, until pH value terminates to ferment when reaching 3.8, obtains fermentation liquid；
J, fermentation liquid obtained by step I is put into storage tank, is then transferred to after-ripening 12 hours in 0~Cool Room 4 DEG C, makes lactic acid bacteria
It further degrades to polymer substances such as albumen, fat in fermentation liquid, generates required small molecule fatty acid, polypeptide and ammonia
Base acid isoreactivity substance；
K, the fermentation liquid for terminating step J after-ripening takes out, and is put into freeze-drying disk, is transferred in vacuum freeze drier,
So that moisture is directly sublimed into gaseous state under the conditions of -10 DEG C of temperature, 1.3~13Pa of pressure, makes material dewatering, sublimation drying is about
24~48 hours, water content was taken out when being 5.0%；Then 10 mesh are crushed to get preventing and improving the drug of senile dementia
1.08Kg。
Sugar is sucrose in the step F.
Lactic acid bacteria in the step I is Lactobacillus casei, streptococcus thermophilus and Lactobacillus rhamnosus, three kinds of strain inoculations
Ratio 1:1.8:1.8.
Using above-mentioned process flow, mainly for the preparation of the drug for preventing and improving senile dementia, extract yield reaches
12.6%, specific effective ingredient yield content may be up to 16.2%.
Embodiment 2
A kind of drug and preparation method thereof preventing senile dementia, comprising the following steps:
A, 200Kg dry American cockroach polypide is crushed, crosses 10 meshes, American cockroach coarse powder is made；
B, the ethyl alcohol of American cockroach coarse powder concentration 95% obtained by step A is impregnated, ethyl alcohol liquid level is made to be higher by coarse powder liquid level 6cm, made
Obtain American cockroach coarse powder mixed liquor；
C, by the investment extractor of American cockroach coarse powder mixed liquor obtained by step B, 70~85 DEG C at a temperature of carry out normal pressure 5 times
Refluxing extraction, inventory, which is less than, extracts the 2/3 of tank volume, carries out first time extraction to American cockroach coarse powder mixed liquor, adds
The ethyl alcohol of 1200Kg concentration 95%, extraction time are 4 hours；The ethyl alcohol for adding 800Kg concentration 95% is extracted after second every time, is mentioned
Taking the time is 3 hours；Combined extract filters to obtain American cockroach ethanol extract；
D, American cockroach ethanol extract obtained by step C is put into single-effect concentrator, in vacuum degree -0.05~-0.06Mpa, temperature
It is concentrated under reduced pressure under conditions of 50~60 DEG C of degree, until obtaining American cockroach ethyl alcohol concentrate when specific gravity is 1.09；
E, American cockroach ethyl alcohol concentrate obtained by step D is moved into degreasing tank, the purified water of 285Kg is added, rises while stirring
Temperature was to constant temperature stirring 50 minutes again after 85~95 DEG C, and mixing speed is 20 revs/min, and then heat preservation, which stands 2 hours, is layered grease,
Take lower layer's aqueous；
F, lower layer's aqueous obtained by step E to be released, centrifugal filtration, filtrate moves into inspissator, at 60~80 DEG C of temperature, vacuum degree-
Be concentrated under reduced pressure under the conditions of 0.05~-0.06MPa, be concentrated into specific gravity be 1.30 when, obtain American-cockroach-extract medicinal extract 26.5Kg；
G, American-cockroach-extract medicinal extract 1Kg obtained by step F is taken by weight, and sugar 0.lKg, water 1Kg are uniformly mixed；It crosses
Filter, filters out insoluble impurities, obtains clear mixed solution；
H, it takes mixing liquid obtained by step G to carry out high-temperature sterilization under the conditions of 100 DEG C of temperature, the time 11 minutes, is subsequently cooled to
25 DEG C, obtain sterilized liquid；
I, sterilized liquid obtained by step H is taken, lactobacillus inoculum, inoculative proportion 1% are then added；Then temperature is controlled 35
It~45 DEG C, seals anaerobic fermentation 12~24 hours, until pH value terminates to ferment when reaching 3.9, obtains fermentation liquid；
J, fermentation liquid obtained by step I is put into storage tank, is then transferred to after-ripening 12 hours in 0~Cool Room 4 DEG C, makes lactic acid bacteria
It further degrades to polymer substances such as albumen, fat in fermentation liquid, generates required small molecule fatty acid, polypeptide and ammonia
Base acid isoreactivity substance；
K, the fermentation liquid for terminating step J after-ripening takes out, and is put into freeze-drying disk, is transferred in vacuum freeze drier,
So that moisture is directly sublimed into gaseous state under the conditions of -20 DEG C of temperature, 1.3~13Pa of pressure, makes material dewatering, sublimation drying is about
24~48 hours, water content was taken out when being 5.0%；Then 10 mesh are crushed to get preventing and improving the drug of senile dementia
1.09Kg。
Sugar is sucrose in the step F.
Lactic acid bacteria in the step I is Lactobacillus casei, streptococcus thermophilus and Lactobacillus rhamnosus, three kinds of strain inoculations
Ratio 1:2.2:2.2.
Using above-mentioned process flow, mainly for the preparation of the drug for preventing and improving senile dementia, extract yield reaches
13.8%, specific effective ingredient yield content may be up to 16.4%.
Embodiment 3
A kind of drug and preparation method thereof preventing senile dementia, comprising the following steps:
A, 200Kg dry American cockroach polypide is crushed, crosses 10 meshes, American cockroach coarse powder is made；
B, the ethyl alcohol of American cockroach coarse powder concentration 92% obtained by step A is impregnated, ethyl alcohol liquid level is made to be higher by coarse powder liquid level 7cm, made
Obtain American cockroach coarse powder mixed liquor；
C, by the investment extractor of American cockroach coarse powder mixed liquor obtained by step B, 70~85 DEG C at a temperature of carry out normal pressure 3 times
Refluxing extraction, inventory, which is less than, extracts the 2/3 of tank volume, carries out first time extraction to American cockroach coarse powder mixed liquor, adds
The ethyl alcohol of 1000Kg concentration 92%, extraction time are 5 hours；It is extracted every time after second and adds 1000Kg times of American cockroach coarse powder
The ethyl alcohol of concentration 92% is measured, extraction time is 4 hours；Combined extract filters to obtain American cockroach ethanol extract；
D, American cockroach ethanol extract obtained by step C is put into single-effect concentrator, in vacuum degree -0.05~-0.06Mpa, temperature
It is concentrated under reduced pressure under conditions of 50~60 DEG C of degree, until obtaining American cockroach ethyl alcohol concentrate when specific gravity is 1.11；
E, American cockroach ethyl alcohol concentrate obtained by step D is moved into degreasing tank, the purified water of 320Kg is added, rises while stirring
Temperature was to constant temperature stirring 40 minutes again after 85~95 DEG C, and mixing speed is 40 revs/min, and then heat preservation, which stands 3 hours, is layered grease,
Take lower layer's aqueous；
F, lower layer's aqueous obtained by step E to be released, centrifugal filtration, filtrate moves into inspissator, at 60~80 DEG C of temperature, vacuum degree-
Be concentrated under reduced pressure under the conditions of 0.05~-0.06MPa, be concentrated into specific gravity be 1.32 when, obtain American-cockroach-extract medicinal extract 27.7Kg；
G, American-cockroach-extract medicinal extract 1Kg obtained by step F is taken by weight, and sugar 0.l5Kg, water 1.5Kg are uniformly mixed；
Filtering, filters out insoluble impurities, obtains clear mixed solution；
H, it takes mixing liquid obtained by step G to carry out high-temperature sterilization under the conditions of 110 DEG C of temperature, the time 12 minutes, is subsequently cooled to
30 DEG C, obtain sterilized liquid；
I, sterilized liquid obtained by step H is taken, lactobacillus inoculum, inoculative proportion 2% are then added；Then temperature is controlled 40
It~42 DEG C, seals anaerobic fermentation 15~20 hours, until pH value terminates to ferment when reaching 4.0, obtains fermentation liquid；
J, fermentation liquid obtained by step I is put into storage tank, is then transferred to after-ripening 24 hours in 0~Cool Room 4 DEG C, makes lactic acid bacteria
It further degrades to polymer substances such as albumen, fat in fermentation liquid, generates required small molecule fatty acid, polypeptide and ammonia
Base acid isoreactivity substance；
K, the fermentation liquid for terminating step J after-ripening takes out, and is put into freeze-drying disk, is transferred in vacuum freeze drier,
So that moisture is directly sublimed into gaseous state under the conditions of -30 DEG C of temperature, 1.3~13Pa of pressure, makes material dewatering, sublimation drying is about
24~48 hours, water content was taken out when being 6.0%；Then 24 mesh are crushed to get preventing and improving the drug of senile dementia
1.13Kg。
Sugar is that glucose is mixed with sucrose by weight 1:l in the step F.
Lactic acid bacteria in the step I is lactobacillus acidophilus, streptococcus thermophilus and lactobacillus bulgaricus, and strain ratio is
1:1:1。
Using above-mentioned process flow, mainly for the preparation of the drug for preventing and improving senile dementia, extract yield reaches
15.50%, specific effective ingredient yield content may be up to 17.20%.
Embodiment 4
A kind of drug and preparation method thereof preventing senile dementia, comprising the following steps:
A, 200Kg dry American cockroach polypide is crushed, crosses 10 meshes, American cockroach coarse powder is made；
B, the ethyl alcohol of American cockroach coarse powder concentration 92% obtained by step A is impregnated, ethyl alcohol liquid level is made to be higher by coarse powder liquid level 8cm, made
Obtain American cockroach coarse powder mixed liquor；
C, by the investment extractor of American cockroach coarse powder mixed liquor obtained by step B, 70~85 DEG C at a temperature of carry out normal pressure 3 times
Refluxing extraction, inventory, which is less than, extracts the 2/3 of tank volume, carries out first time extraction to American cockroach coarse powder mixed liquor, adds
The ethyl alcohol of 1000Kg concentration 92%, extraction time are 5 hours；The ethyl alcohol for adding 1000Kg concentration 92% is extracted after second every time,
Extraction time is 4 hours；Combined extract filters to obtain American cockroach ethanol extract；
D, American cockroach ethanol extract obtained by step C is put into single-effect concentrator, in vacuum degree -0.05~-0.06Mpa, temperature
It is concentrated under reduced pressure under conditions of 50~60 DEG C of degree, until obtaining American cockroach ethyl alcohol concentrate when specific gravity is 1.12；
E, American cockroach ethyl alcohol concentrate obtained by step D is moved into degreasing tank, the purified water of 318Kg is added, rises while stirring
Temperature was to constant temperature stirring 30 minutes again after 85~95 DEG C, and mixing speed is 40 revs/min, and then heat preservation, which stands 4 hours, is layered grease,
Take lower layer's aqueous；
F, lower layer's aqueous obtained by step E to be released, centrifugal filtration, filtrate moves into inspissator, at 60~80 DEG C of temperature, vacuum degree-
Be concentrated under reduced pressure under the conditions of 0.05~-0.06MPa, be concentrated into specific gravity be 1.32 when, obtain American-cockroach-extract medicinal extract 31.4Kg；
G, American-cockroach-extract medicinal extract 1Kg, sugar 0.l5Kg, 1.5 Kg of water obtained by step F are taken by weight, are stirred
It is even；Filtering, filters out insoluble impurities, obtains clear mixed solution；
H, it takes mixing liquid obtained by step G to carry out high-temperature sterilization under the conditions of 110 DEG C of temperature, the time 13 minutes, is subsequently cooled to
35 DEG C, obtain sterilized liquid；
I, sterilized liquid obtained by step H is taken, lactobacillus inoculum, inoculative proportion 3% are then added；Then temperature is controlled 40
It~42 DEG C, seals anaerobic fermentation 15~20 hours, until pH value terminates to ferment when reaching 4.2, obtains fermentation liquid；
J, fermentation liquid obtained by step I is put into storage tank, is then transferred to after-ripening 24 hours in 0~Cool Room 4 DEG C, makes lactic acid bacteria
It further degrades to polymer substances such as albumen, fat in fermentation liquid, generates required small molecule fatty acid, polypeptide and ammonia
Base acid isoreactivity substance；
K, the fermentation liquid for terminating step J after-ripening takes out, and is put into freeze-drying disk, is transferred in vacuum freeze drier,
So that moisture is directly sublimed into gaseous state under the conditions of -40 DEG C of temperature, 1.3~13Pa of pressure, makes material dewatering, sublimation drying is about
24~48 hours, water content was taken out when being 7.0%；Then 24 mesh are crushed to get preventing and improving the drug of senile dementia
1.14Kg。
Sugar is that glucose is mixed with sucrose by weight 1:l in the step F.
Lactic acid bacteria in the step I is lactobacillus acidophilus, streptococcus thermophilus and lactobacillus bulgaricus, and strain ratio is
1: 2.0:1。
Using above-mentioned process flow, mainly for the preparation of the drug for preventing and improving senile dementia, extract yield reaches
15.8%, specific effective ingredient yield content may be up to 17.23%.
Embodiment 5
A kind of drug and preparation method thereof preventing senile dementia, comprising the following steps:
A, 200Kg dry American cockroach polypide is crushed, crosses 10 meshes, American cockroach coarse powder is made；
B, the ethyl alcohol of American cockroach coarse powder concentration 90% obtained by step A is impregnated, ethyl alcohol liquid level is made to be higher by coarse powder liquid level 9cm, made
Obtain American cockroach coarse powder mixed liquor；
C, by the investment extractor of American cockroach coarse powder mixed liquor obtained by step B, 70~85 DEG C at a temperature of carry out normal pressure 4 times
Refluxing extraction, inventory, which is less than, extracts the 2/3 of tank volume, carries out first time extraction to American cockroach coarse powder mixed liquor, adds
The ethyl alcohol of 1400Kg concentration 90%, extraction time are 6 hours；The ethyl alcohol for adding 1000Kg concentration 90% is extracted after second every time,
Extraction time is 5 hours；Combined extract filters to obtain American cockroach ethanol extract；
D, American cockroach ethanol extract obtained by step C is put into single-effect concentrator, in vacuum degree -0.05~-0.06Mpa, temperature
It is concentrated under reduced pressure under conditions of 50~60 DEG C of degree, until obtaining American cockroach ethyl alcohol concentrate when specific gravity is 1.13；
E, American cockroach ethyl alcohol concentrate obtained by step D is moved into degreasing tank, the purified water of 366Kg is added, rises while stirring
Temperature was to constant temperature stirring 20 minutes again after 85~95 DEG C, and mixing speed is 60 revs/min, and then heat preservation, which stands 5 hours, is layered grease,
Take lower layer's aqueous；
F, lower layer's aqueous obtained by step E to be released, centrifugal filtration, filtrate moves into inspissator, at 60~80 DEG C of temperature, vacuum degree-
Be concentrated under reduced pressure under the conditions of 0.05~-0.06MPa, be concentrated into specific gravity be 1.27 when, obtain American-cockroach-extract medicinal extract 31.2Kg；
G, American-cockroach-extract medicinal extract 1Kg obtained by step F is taken by weight, and sugar 0.2Kg, 2 Kg of water are uniformly mixed；It crosses
Filter, filters out insoluble impurities, obtains clear mixed solution；
H, it takes mixing liquid obtained by step G to carry out high-temperature sterilization under the conditions of 120 DEG C of temperature, the time 14 minutes, is subsequently cooled to
40 DEG C, obtain sterilized liquid；
I, sterilized liquid obtained by step H is taken, lactobacillus inoculum, inoculative proportion 4% are then added；Then temperature is controlled 35
It~45 DEG C, seals anaerobic fermentation 12~24 hours, until pH value terminates to ferment when reaching 4.4, obtains fermentation liquid；
J, fermentation liquid obtained by step I is put into storage tank, is then transferred to after-ripening 36 hours in 0~Cool Room 4 DEG C, makes lactic acid bacteria
It further degrades to polymer substances such as albumen, fat in fermentation liquid, generates required small molecule fatty acid, polypeptide and ammonia
Base acid isoreactivity substance；
K, the fermentation liquid for terminating step J after-ripening takes out, and is put into freeze-drying disk, is transferred in vacuum freeze drier,
So that moisture is directly sublimed into gaseous state under the conditions of -50 DEG C of temperature, 1.3~13Pa of pressure, makes material dewatering, sublimation drying is about
24~48 hours, water content was taken out when being 8.0%；Then 40 mesh are crushed to get preventing and improving the drug of senile dementia
1.17Kg。
Sugar is that glucose is mixed with sucrose by weight 1:2 in the step F.
Lactic acid bacteria in the step I is lactobacillus plantarum, streptococcus thermophilus and Lactobacillus delbrueckii subspecies, and strain ratio is
1:0.8:0.8。
Using above-mentioned process flow, mainly for the preparation of the drug for preventing and improving senile dementia, extract yield reaches
16.02%, specific effective ingredient yield content may be up to 15.8%.
Embodiment 6
A kind of drug and preparation method thereof preventing senile dementia, comprising the following steps:
A, 200Kg dry American cockroach polypide is crushed, crosses 10 meshes, American cockroach coarse powder is made；
B, the ethyl alcohol of American cockroach coarse powder concentration 90% obtained by step A is impregnated, ethyl alcohol liquid level is made to be higher by coarse powder liquid level 10cm,
American cockroach coarse powder mixed liquor is made；
C, by the investment extractor of American cockroach coarse powder mixed liquor obtained by step B, 70~85 DEG C at a temperature of carry out normal pressure 4 times
Refluxing extraction, inventory, which is less than, extracts the 2/3 of tank volume, carries out first time extraction to American cockroach coarse powder mixed liquor, adds
The ethyl alcohol of 1600Kg concentration 90%, extraction time are 6 hours；The ethyl alcohol for adding 1200Kg concentration 90% is extracted after second every time,
Extraction time is 5 hours；Combined extract filters to obtain American cockroach ethanol extract；
D, American cockroach ethanol extract obtained by step C is put into single-effect concentrator, in vacuum degree -0.05~-0.06Mpa, temperature
It is concentrated under reduced pressure under conditions of 50~60 DEG C of degree, until obtaining American cockroach ethyl alcohol concentrate when specific gravity is 1.14；
E, American cockroach ethyl alcohol concentrate obtained by step D is moved into degreasing tank, the purified water of 398Kg is added, rises while stirring
Temperature was to constant temperature stirring 10 minutes again after 85~95 DEG C, and mixing speed is 60 revs/min, and then heat preservation, which stands 5 hours, is layered grease,
Take lower layer's aqueous；
F, lower layer's aqueous obtained by step E to be released, centrifugal filtration, filtrate moves into inspissator, at 60~80 DEG C of temperature, vacuum degree-
Be concentrated under reduced pressure under the conditions of 0.05~-0.06MPa, be concentrated into specific gravity be 1.27 when, obtain American-cockroach-extract medicinal extract 31.3Kg；
G, American-cockroach-extract medicinal extract 1Kg obtained by step F is taken by weight, and sugar 0.2Kg, 2 Kg of water are uniformly mixed；It crosses
Filter, filters out insoluble impurities, obtains clear mixed solution；
H, it takes mixing liquid obtained by step G to carry out high-temperature sterilization under the conditions of 120 DEG C of temperature, the time 15 minutes, is subsequently cooled to
45 DEG C, obtain sterilized liquid；
I, sterilized liquid obtained by step H is taken, lactobacillus inoculum, inoculative proportion 5% are then added；Then temperature is controlled 35
It~45 DEG C, seals anaerobic fermentation 12~24 hours, until pH value terminates to ferment when reaching 4.5, obtains fermentation liquid；
J, fermentation liquid obtained by step I is put into storage tank, is then transferred to after-ripening 36 hours in 0~Cool Room 4 DEG C, makes lactic acid bacteria
It further degrades to polymer substances such as albumen, fat in fermentation liquid, generates required small molecule fatty acid, polypeptide and ammonia
Base acid isoreactivity substance；
K, the fermentation liquid for terminating step J after-ripening takes out, and is put into freeze-drying disk, is transferred in vacuum freeze drier,
So that moisture is directly sublimed into gaseous state under the conditions of -50 DEG C of temperature, 1.3~13Pa of pressure, makes material dewatering, sublimation drying is about
24~48 hours, water content was taken out when being 8.0%；Then 40 mesh are crushed to get preventing and improving the drug of senile dementia
1.18Kg。
Sugar is that glucose is mixed with sucrose by weight 1:2 in the step F.
Lactobacillus plantarum, streptococcus thermophilus and Lactobacillus delbrueckii subspecies in the step I, strain ratio are 1:1.2:
1.2。
Using above-mentioned process flow, mainly for the preparation of the drug for preventing and improving senile dementia, extract yield reaches
16.20%, specific effective ingredient yield content may be up to 15.50%.
Embodiment 7
A kind of drug and preparation method thereof preventing senile dementia, comprising the following steps:
A, 200Kg dry American cockroach polypide is crushed, crosses 10 meshes, American cockroach coarse powder is made；
B, the ethyl alcohol of American cockroach coarse powder concentration 92% obtained by step A is impregnated, ethyl alcohol liquid level is made to be higher by coarse powder liquid level 7cm, made
Obtain American cockroach coarse powder mixed liquor；
C, by the investment extractor of American cockroach coarse powder mixed liquor obtained by step B, 70~85 DEG C at a temperature of carry out normal pressure 3 times
Refluxing extraction, inventory, which is less than, extracts the 2/3 of tank volume, carries out first time extraction to American cockroach coarse powder mixed liquor, adds
The ethyl alcohol of 1000Kg concentration 92%, extraction time are 5 hours；The ethyl alcohol for adding 1000Kg concentration 92% is extracted after second every time,
Extraction time is 4 hours；Combined extract filters to obtain American cockroach ethanol extract；
D, American cockroach ethanol extract obtained by step C is put into single-effect concentrator, in vacuum degree -0.05~-0.06Mpa, temperature
It is concentrated under reduced pressure under conditions of 50~60 DEG C of degree, until obtaining American cockroach ethyl alcohol concentrate when specific gravity is 1.05；
E, American cockroach ethyl alcohol concentrate obtained by step D is moved into degreasing tank, the purified water of 322Kg is added, rises while stirring
Temperature was to constant temperature stirring 30 minutes again after 85~95 DEG C, and mixing speed is 40 revs/min, and then heat preservation, which stands 3 hours, is layered grease,
Take lower layer's aqueous；
F, lower layer's aqueous obtained by step E to be released, centrifugal filtration, filtrate moves into inspissator, at 60~80 DEG C of temperature, vacuum degree-
Be concentrated under reduced pressure under the conditions of 0.05~-0.06MPa, be concentrated into specific gravity be 1.32 when, obtain American-cockroach-extract medicinal extract 27.7Kg；
G, American-cockroach-extract medicinal extract 1Kg, sugar 0.l5Kg, 1.5 Kg of water obtained by step F are taken by weight, are stirred
It is even；Filtering, filters out insoluble impurities, obtains clear mixed solution；
H, it takes mixing liquid obtained by step G to carry out high-temperature sterilization under the conditions of 110 DEG C of temperature, the time 12 minutes, is subsequently cooled to
30 DEG C, obtain sterilized liquid；
I, sterilized liquid obtained by step H is taken, lactobacillus inoculum, inoculative proportion 2% are then added；Then temperature is controlled 40
It~42 DEG C, seals anaerobic fermentation 15~20 hours, until pH value terminates to ferment when reaching 4.0, obtains fermentation liquid；
J, fermentation liquid obtained by step I is put into storage tank, is then transferred to after-ripening 24 hours in 0~Cool Room 4 DEG C, makes lactic acid bacteria
It further degrades to polymer substances such as albumen, fat in fermentation liquid, generates required small molecule fatty acid, polypeptide and ammonia
Base acid isoreactivity substance；
K, the fermentation liquid for terminating step J after-ripening takes out, and is put into freeze-drying disk, is transferred in vacuum freeze drier,
So that moisture is directly sublimed into gaseous state under the conditions of -30 DEG C of temperature, 1.3~13Pa of pressure, makes material dewatering, sublimation drying is about
24~48 hours, water content was taken out when being 6.0%；Then 24 mesh are crushed to get preventing and improving the drug of senile dementia
1.11Kg。
Sugar is that glucose is mixed with sucrose by weight 1:l in the step F.
Lactic acid bacteria in the step I is lactobacillus paracasei.
Using above-mentioned process flow, mainly for the preparation of the drug for preventing and improving senile dementia, extract yield reaches
14.20%, specific effective ingredient yield content may be up to 16.80%.
Embodiment 8
A kind of drug and preparation method thereof preventing senile dementia, comprising the following steps:
A, 200Kg dry American cockroach polypide is crushed, crosses 10 meshes, American cockroach coarse powder is made；
B, the ethyl alcohol of American cockroach coarse powder concentration 92% obtained by step A is impregnated, ethyl alcohol liquid level is made to be higher by coarse powder liquid level 8cm, made
Obtain American cockroach coarse powder mixed liquor；
C, by the investment extractor of American cockroach coarse powder mixed liquor obtained by step B, 70~85 DEG C at a temperature of carry out normal pressure 3 times
Refluxing extraction, inventory, which is less than, extracts the 2/3 of tank volume, carries out first time extraction to American cockroach coarse powder mixed liquor, adds
The ethyl alcohol of 1000Kg concentration 92%, extraction time are 5 hours；The ethyl alcohol for adding 1000Kg concentration 92% is extracted after second every time,
Extraction time is 4 hours；Combined extract filters to obtain American cockroach ethanol extract；
D, American cockroach ethanol extract obtained by step C is put into single-effect concentrator, in vacuum degree -0.05~-0.06Mpa, temperature
It is concentrated under reduced pressure under conditions of 50~60 DEG C of degree, until obtaining American cockroach ethyl alcohol concentrate when specific gravity is 1.15；
E, American cockroach ethyl alcohol concentrate obtained by step D is moved into degreasing tank, the purified water of 332Kg is added, rises while stirring
Temperature was to constant temperature stirring 40 minutes again after 85~95 DEG C, and mixing speed is 40 revs/min, and then heat preservation, which stands 4 hours, is layered grease,
Take lower layer's aqueous；
F, lower layer's aqueous obtained by step E to be released, centrifugal filtration, filtrate moves into inspissator, at 60~80 DEG C of temperature, vacuum degree-
Be concentrated under reduced pressure under the conditions of 0.05~-0.06MPa, be concentrated into specific gravity be 1.32 when, obtain American-cockroach-extract medicinal extract 31.4Kg；
G, American-cockroach-extract medicinal extract 1Kg, sugar 0.15Kg, 1.5 Kg of water obtained by step F are taken by weight, are stirred
It is even；Filtering, filters out insoluble impurities, obtains clear mixed solution；
H, it takes mixing liquid obtained by step G to carry out high-temperature sterilization under the conditions of 110 DEG C of temperature, the time 13 minutes, is subsequently cooled to
35 DEG C, obtain sterilized liquid；
I, sterilized liquid obtained by step H is taken, lactobacillus inoculum, inoculative proportion 3% are then added；Then temperature is controlled 40
It~42 DEG C, seals anaerobic fermentation 15~20 hours, until pH value terminates to ferment when reaching 4.2, obtains fermentation liquid；
J, fermentation liquid obtained by step I is put into storage tank, is then transferred to after-ripening 24 hours in 0~Cool Room 4 DEG C, makes lactic acid bacteria
It further degrades to polymer substances such as albumen, fat in fermentation liquid, generates required small molecule fatty acid, polypeptide and ammonia
Base acid isoreactivity substance；
K, the fermentation liquid for terminating step J after-ripening takes out, and is put into freeze-drying disk, is transferred in vacuum freeze drier,
So that moisture is directly sublimed into gaseous state under the conditions of -40 DEG C of temperature, 1.3~13Pa of pressure, makes material dewatering, sublimation drying is about
24~48 hours, water content was taken out when being 7.0%；Then 24 mesh are crushed to get preventing and improving the drug of senile dementia
1.10Kg。
Sugar is that glucose is mixed with sucrose by weight 1:l in the step F.
Lactic acid bacteria in the step I is Roy's formula lactobacillus.
Using above-mentioned process flow, mainly for the preparation of the drug for preventing and improving senile dementia, extract yield reaches
14.50%, specific effective ingredient yield content may be up to 16.60%.
Embodiment 9
It is that the present invention prevents and the drug of improvement senile dementia declines hypomnesia, judgement, is apathy, anti-below
Answer the improvement result of the symptoms such as blunt:
Case-data and grouping: 86, slight senile dementia is diagnosed as through hospital；It is divided into treatment group 47 and control group 39.
Treatment group male 28, female 19；Age 50 ~ 90 years old；The course of disease 6 months ~ 5 years；Hypomnesia 21 (44.7%), under judgement
14 (29.8%) drops, and it is 12 (25.5%) apathy, slow in reacting.Control group male 23, female 16；Age 50 ~ 90 years old；Disease
Journey 6 months ~ 3 years；Hypomnesia 16 (41%), judgement 12 (30.8%) of decline, 11 apathy, slow in reacting
(28.2%).
Method: control group takes orally Ares (donepezil hydrochloride tablet), treatment dosage 5mg/ times (1 times a day), sleeps every night
It is preceding oral, it takes one month.Treatment group is taken after mixing it with water using the drug 10g boiled water of present invention prevention and improvement senile dementia, and daily 3
It is secondary, sooner or later before meals and each 1 time before sleeping；It takes one month, duration of medication quits smoking, wine and vinegar-pepper irritable food.2 after treatment end
It is checked in all to hospital.
Curative effect judging standard: (1) effective: the above symptom all disappears, and memory is remarkably reinforced, can divide event
Analysis, thinking, judgement, have corresponding emotional responses to environmental stimuli, begin to focus on and current events around occur, and reaction is quick；(2) have
Effect: the above symptom is obviously improved, and memory, judgement, emotion and action reaction, which have, to be obviously improved；(3) invalid: more than
Symptom does not mitigate or aggravates, and memory is badly damaged, and loses judgement, and emotion becomes irritable uneasy from indifferent, often walks about and do not stop,
It can be seen that the urinary incontinence.Obvious effective rate+effective percentage=total effective rate.
Test data (is shown in Table 1, wherein n number of delegates, percentages, which are taken to round up, retains one decimal place)
1: two group of curative effect of table compares
As a result: after active treatment, the obvious effective rate and total effective rate for the treatment of group patient is obviously higher than control group.
Embodiment 10
Be below the present invention prevention and improve senile dementia drug be badly damaged to memory, cognitive disorder, aphasis, think of
Tie up the improvement result of the symptoms such as obstacle, disorientation, self care ability obstacle:
Case-data and grouping: 92, middle heavy senile dementia is diagnosed as through hospital；Wherein male 53, female 39；Age 50 ~ 90
Year, average age (70 ± 15) year；The course of disease 2 ~ 12 years, average course of disease (7 ± 4) year；The equal voluntary participation of selected patient this
Research, and informed consent form is signed with patient.It is randomly divided into treatment group and control group, every group each 46, the year of two groups of patients
Age, gender and the equal no significant difference of the course of disease, are comparable (P > 0.05).
Method: control group oral hydrochloride U.S. dollar tablet, the dosage for the treatment of first week are daily 5mg(half, morning clothes), second
All each half of 10mg(daily, twice daily), third week, 15mg(took a piece of morning daily, and take half in the afternoon), the 4th week starts
The later daily 20mg(of maintenance dose each one, twice daily), treat two months.Treatment group is using present invention prevention and improves
The drug 10g boiled water of senile dementia is taken after mixing it with water, and 3 times a day, sooner or later before meals and each 1 time before sleeping, the course for the treatment of is one month, continuous use
2 courses for the treatment of, duration of medication quits smoking, wine and vinegar-pepper irritable food.It is answered to hospital within 2 weeks or so after two groups of patient's treatment ends
It looks into.
Curative effect judging standard: (1) effective: the above symptom all disappears, and memory is remarkably reinforced, and cognition, language competence are extensive
Multiple, thinking is clear, and visual space ability is remarkably reinforced, and can make orientation to time, place and personage, restore self care ability；
(2) effectively: the above symptom is obviously improved, and memory, cognition, language competence, which have, to be obviously improved, and the disturbance of thought makes moderate progress, extensive
The visual space ability of multiple simple structure, time, place and disturbance of orientation to person improve, and self care ability makes moderate progress；(3) nothing
Effect: the above symptom does not mitigate or aggravates, and serious memory loss completely loses self-care ability.Obvious effective rate+effective percentage=total effective
Rate.
Test data (is shown in Table 2, wherein n number of delegates, percentages, which are taken to round up, retains one decimal place)
2: two groups of curative effects of table compare
As a result: after active treatment, the obvious effective rate and total effective rate for the treatment of group patient is obviously higher than control group.
Claims (7)
1. a kind of drug and preparation method thereof for preventing senile dementia, which comprises the following steps:
A, dry American cockroach polypide is crushed, crosses 10 meshes, American cockroach coarse powder is made；
B, the ethyl alcohol of American cockroach coarse powder concentration 90%~95% obtained by step A is impregnated, ethyl alcohol liquid level is made to be higher by coarse powder liquid level 5
American cockroach coarse powder mixed liquor is made in~10cm；
C, by the investment extractor of American cockroach coarse powder mixed liquor obtained by step B, 70~85 DEG C at a temperature of carry out normal pressure 3~
5 refluxing extractions, inventory, which is less than, extracts the 2/3 of tank volume, carries out first time extraction to American cockroach coarse powder mixed liquor, adds beauty
The ethyl alcohol of 5~8 times of the big Lian coarse powder in continent amount concentration 90%~95%, extraction time are 4~6 hours；It extracts and adds every time after second
The ethyl alcohol of 3~6 times of American cockroach coarse powder amount concentration 90%~95%, extraction time are 3~5 hours；Combined extract filters beautiful
The big Lian ethanol extract in continent；
D, American cockroach ethanol extract obtained by step C is put into single-effect concentrator, in vacuum degree -0.05~-0.06Mpa, temperature
It is concentrated under reduced pressure under conditions of 50~60 DEG C of degree, until obtaining American cockroach ethyl alcohol concentrate when specific gravity is 1.05~1.15；
E, American cockroach ethyl alcohol concentrate obtained by step D is moved into degreasing tank, addition American cockroach ethyl alcohol concentrate weight 7~
The purified water of 10 times of amounts, to constant temperature stirring 10~60 minutes again after 85~95 DEG C, mixing speed was 20~60 for warming while stirring
Rev/min, then heat preservation, which stands 1~5 hour, is layered grease, takes lower layer's aqueous；
F, lower layer's aqueous obtained by step E to be released, centrifugal filtration, filtrate moves into inspissator, at 60~80 DEG C of temperature, vacuum degree-
Be concentrated under reduced pressure under the conditions of 0.05~-0.06MPa, be concentrated into specific gravity be 1.25~1.35 when, obtain American-cockroach-extract medicinal extract；
G, American-cockroach-extract medicinal extract 1 part is taken obtained by step F by weight, and sugar 0.l~0.2 part, is stirred by 1~2 part of water
Uniformly；Filtering, filters out insoluble impurities, obtains clear mixed solution；
H, mixing liquid obtained by step G is taken to carry out high-temperature sterilization under the conditions of 100~120 DEG C of temperature, the time 10~15 minutes, so
After be cooled to 25~45 DEG C, obtain sterilized liquid；
I, sterilized liquid obtained by step H is taken, lactobacillus inoculum is then added, inoculative proportion is 0.5%~5%；Then by temperature control
System seals anaerobic fermentation 12~24 hours at 35~45 DEG C, until pH value terminates to ferment when reaching 3.8~4.5, is fermented
Liquid；
J, fermentation liquid obtained by step I is put into storage tank, is then transferred to after-ripening 12~36 hours in 0~Cool Room 4 DEG C, makes cream
Sour bacterium further degrades to polymer substances such as albumen, fat in fermentation liquid, generate required small molecule fatty acid, polypeptide with
And amino acid isoreactivity substance；
K, the fermentation liquid for terminating step J after-ripening takes out, and is put into freeze-drying disk, is transferred in vacuum freeze drier,
So that moisture is directly sublimed into gaseous state under the conditions of temperature -10~-50 DEG C, 1.3~13Pa of pressure, make material dewatering, when freeze-drying
Between about 24~48 hours, water content be 5.0%~8.0% when take out；Then 10~40 mesh are crushed to get preventing and improving old age
Dull-witted drug.
2. a kind of drug and preparation method thereof for preventing senile dementia according to claim 1, which is characterized in that the step
Rapid C can use following technical scheme: American cockroach coarse powder mixed liquor obtained by step B be put into extractor, at 70~85 DEG C
At a temperature of carry out 3 refluxing extractions of normal pressure, inventory be less than extract tank volume 2/3, to American cockroach coarse powder mixed liquor into
Row extracts for the first time, adds the ethyl alcohol of 5 times of American cockroach coarse powder amount concentration 92%, and extraction time is 5 hours；Second of extraction plus beauty
The ethyl alcohol of 5 times of the big Lian coarse powder in continent amount concentration 92%, extraction time are 4 hours；Third time is extracted plus 5 times of amounts of American cockroach coarse powder are dense
The ethyl alcohol of degree 92%, extraction time are 4 hours；Merge extracting solution three times, filters to obtain American cockroach ethanol extract.
3. a kind of drug and preparation method thereof for preventing senile dementia according to claim 1, which is characterized in that the step
Rapid E can use following technical scheme: American cockroach ethyl alcohol concentrate obtained by step D being moved into degreasing tank, it is big that America is added
The purified water of 8 times of Lian ethyl alcohol concentrate weight amounts, to constant temperature stirring 40 minutes again after 85~95 DEG C, stirring was fast for warming while stirring
Degree is 40 revs/min, and then heat preservation, which stands 4 hours, is layered grease, takes lower layer's aqueous.
4. a kind of drug and preparation method thereof for preventing senile dementia according to claim 1, which is characterized in that the step
Sugar is that sucrose or glucose are mixed with sucrose by weight 1:l~2 in rapid F.
5. a kind of drug and preparation method thereof for preventing senile dementia according to claim 1, which is characterized in that the step
Lactic acid bacteria in rapid I is selected from Lactobacillus casei, streptococcus thermophilus, Lactobacillus rhamnosus, lactobacillus plantarum, lactobacillus acidophilus, guarantor
Add one or more of Leah lactobacillus, lactobacillus delbruockii subspecies bulgaricus, lactobacillus paracasei and Roy's formula lactobacillus.
6. a kind of drug and preparation method thereof for preventing senile dementia according to claim 1, which is characterized in that the step
Lactic acid bacteria in rapid I is Lactobacillus casei, streptococcus thermophilus and Lactobacillus rhamnosus, three kinds of strain inoculative proportion 1:1.8~
2.2:1.8~2.2；Or lactic acid bacteria is lactobacillus acidophilus, streptococcus thermophilus and lactobacillus bulgaricus, strain ratio is 1:
1.0~2.0:1；Or lactic acid bacteria is lactobacillus plantarum, streptococcus thermophilus and Lactobacillus delbrueckii subspecies, strain ratio is 1:0.8
~1.2:0.8~1.2.
7. a kind of drug and preparation method thereof for preventing senile dementia according to claim 1, which is characterized in that the step
Lactobacillus inoculum ratio in rapid I is 2%~3%, and at 40~42 DEG C, fermentation time control is small 15~20 for fermentation temperature control
When, fermentation ends control point pH value is 4.0~4.2.
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| CN113274413A (en) * | 2021-07-01 | 2021-08-20 | 大理大学 | Periplaneta americana effective part extract, and extraction method, application and pharmaceutical composition thereof |
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