CN110050796B

CN110050796B - Pesticide for preventing and treating bemisia tabaci and using method thereof

Info

Publication number: CN110050796B
Application number: CN201910386160.7A
Authority: CN
Inventors: 张安盛; 邢光耀; 周仙红; 张秀霞; 庄乾营; 于毅
Original assignee: Institute of Plant Protection Shandong Academy of Agricultural Sciences
Current assignee: Institute of Plant Protection Shandong Academy of Agricultural Sciences
Priority date: 2019-05-09
Filing date: 2019-05-09
Publication date: 2021-07-02
Anticipated expiration: 2039-05-09
Also published as: CN110050796A

Abstract

The invention belongs to the technical field of pesticides, and particularly relates to an insecticide for preventing and treating bemisia tabaci and a using method thereof, namely a deltamethrin and guadipyr compound insecticide and a using method thereof. The pesticide for preventing and treating bemisia tabaci comprises the following raw materials in percentage by weight: deltamethrin and guadipyr account for 2.0 percent of the total weight, the auxiliary agent accounts for 5.0 to 15.0 percent, and the filler carrier accounts for 83.0 to 93.0 percent. The insecticidal composition adopts two medicaments as main components, and is matched with an auxiliary agent and a filler carrier, so that the effects of complementary advantages and complementary effects are achieved. The method has the advantages of simple process, high yield, no generation of three wastes and effective improvement of the control effect on the bemisia tabaci, and all the input raw materials are converted into products.

Description

Pesticide for preventing and treating bemisia tabaci and using method thereof

Technical Field

The invention belongs to the technical field of pesticides, particularly relates to an insecticide for preventing and treating bemisia tabaci and a using method thereof, and particularly relates to a deltamethrin and guadipyr compound insecticide and a using method thereof.

Background

Bemisia tabaci (Bemisia tabaci) belongs to the family Bemisia, Hemisalidae, and is an important pest of crops. The pest has wide host range, and can damage vegetables, cotton, tobacco, etc. Bemisia tabaci is mainly harmful to the back of host leaves, plant juice is absorbed by thorns, plants are weak, nymphs and adults can also secrete honeydew to induce the generation of sooty mould, and when the density is high, leaves are black, so that photosynthesis is seriously influenced; besides direct damage, bemisia tabaci also spreads a variety of viral diseases, seriously affecting host plant yield and quality. The aphid has the characteristics of high diffusion speed, high reproduction rate, high resistance increase and the like, and is difficult to be effectively controlled by common chemical agents.

Currently, the medicaments for preventing and controlling bemisia tabaci produced mainly comprise clothianidin, imidacloprid, acetamiprid, beta-cypermethrin, beta-cyhalothrin, thiamethoxam and other compound medicaments, but the prevention and control effect on the bemisia tabaci is not ideal. The pesticide is used unscientific for a long time, so that the resistance of the bemisia tabaci is enhanced, and the harm of the bemisia tabaci cannot be effectively controlled despite the continuous increase of the dosage.

Deltamethrin, the common name in English, is Deltamethrin, is a pyrethroid insecticide. The chemical name of the compound is "(S) -alpha-cyanophenoxybenzyl (1R, 3R) -3- (2, 2-dibromoethenyl) -2, 2-dimethylcyclopropane carboxylate", and the structural formula is as follows:

the Guadipyr is a neonicotinoid insecticide with a novel structure developed by Chinese agriculture university, and is a novel efficient insecticidal series compound which is obtained by combining the active structures of neonicotinoids (represented by imidacloprid) and semicarbazone insecticides (represented by indoxacarb) into the same molecule and has the characteristics of neonicotinoids and sodium ion channel inhibitors. The chemical name is 1-nitro-3- [ (6-chloropyridin-3-yl) methyl ] -4-pentamethylene aminoguanidine', and the structural formula is as follows:

at present, the report of the combination of deltamethrin and guadipyr for preventing and controlling bemisia tabaci does not exist.

Disclosure of Invention

In order to solve the problems, the applicant finds that the deltamethrin and the guadipyr are compounded according to a specific proportion to show a remarkable synergistic effect on the basis of intensive research, and the obtained novel pesticide has a good control effect on bemisia tabaci.

The pesticide for preventing and treating bemisia tabaci comprises the following raw materials in percentage by weight: 2.0 percent of deltamethrin and guadipyr, 5.0 to 15.0 percent of auxiliary agent and 83.0 to 93.0 percent of filler carrier.

In the invention, the insecticidal mechanism of the deltamethrin is as follows: acts on a sodium ion channel of a nerve membrane of the pest, simultaneously inhibits the activation of sodium ions and the passivation of sodium ions and potassium ions, ensures that the action point position cannot reach a normal value, prolongs the negative post potential, causes repeated radiation, continuously excites the insect, finally generates neurotoxin, and leads the pest to die. The preparation has contact poisoning and stomach poisoning effects, has certain repelling and antifeedant effects, and has good control effect on various pests such as Hemiptera (Bemisia tabaci, aphid, etc.), Lepidoptera (Heliothis armigera, beet armyworm, etc.). The drug resistance monitoring shows that when the pesticide is used independently, pests are easy to generate drug resistance, so that the cost performance of controlling the intractable pest bemisia tabaci is remarkably reduced.

In the invention, the insecticidal mechanism of the guadipyr is as follows: selectively inhibit nicotinic acid acetylcholinesterase receptors of central nervous systems of pests and block sodium ion channels in insect nerve cells, and the insecticidal composition has multiple action targets, so that normal conduction of central nerves of the pests is blocked, and nerve cells lose functions. The pesticide has good systemic effect, and has good control effect on various pests such as Hemiptera (whitefly, aphid, etc.), Lepidoptera (cotton bollworm, beet armyworm, etc.). The drug resistance monitoring shows that when the pesticide is used alone, pests are easy to generate drug resistance, so that the cost performance of controlling intractable pests is obviously reduced.

In the invention, when the two substances are combined, because the deltamethrin and the guadipyr have different action modes, the advantages are complemented, and the control effect on the bemisia tabaci is effectively improved.

The auxiliary agent is a compound agricultural auxiliary agent and is a mixture of 2-naphthalene sulfonic acid formaldehyde polymer sodium salt (96% in Wuhan, a product of Co-creation science and technology Co., Ltd.), sodium dodecyl sulfate (98% in Ornkian synthetic chemistry Co., Ltd.) and nekal (98% in Zhengzhou Chengnio chemical products Co., Ltd.). The sodium salt of the 2-naphthalenesulfonic acid formaldehyde polymer plays a role of a dispersing agent, the lauryl sodium sulfate plays a role of wetting and permeating, the tobacco whitefly and crops can absorb effective components of the composition, the surface tension of water can be obviously reduced by the aid of the nefara, the nefara has excellent penetrating power and wetting performance, the rewettability is also excellent, and the composition has emulsifying, diffusing and foaming performances. The sodium salt of the 2-naphthalenesulfonic acid formaldehyde polymer, the sodium dodecyl sulfate and the nekal can be scientifically compounded and used, can effectively play roles in dispersing, wetting, permeating and suspending, and is beneficial to the absorption of the active ingredients of the composition by bemisia tabaci and crops. The higher the absorption rate of the effective components of the composition by the bemisia tabaci is, the better the killing effect on the bemisia tabaci is; the higher the absorption rate of the effective components of the composition by crops, the higher the killing effect of the bemisia tabaci during the process of puncturing the cells of the crops to suck the juice. The auxiliary agent improves the control effect of the composition on bemisia tabaci, can effectively control bemisia tabaci with high reproduction rate, increased resistance and difficult control, realizes the pesticide reduction control of the pests, and has important significance. The raw materials added by the aid are all converted into products, the yield is high, three wastes are not generated, and the control effect on the bemisia tabaci is effectively improved. The sodium salt of the 2-naphthalenesulfonic acid formaldehyde polymer accounts for 6.0% of the total weight of the pesticide; the sodium dodecyl sulfate accounts for 1.8 percent of the total weight of the pesticide; the nekal accounts for 2.2% of the total weight of the pesticide.

The filler carrier is diatomite (purchased from Kede chemical Co., Ltd. of Shouguang), and can be widely used in industrial production due to excellent adsorption performance.

The invention is characterized in that deltamethrin and guadipyr are respectively mixed according to the weight ratio of 9.0:1.0,7.0: 3.0,5.0: 5.0,3.0: 7.0,1.0: 9.0, compounding and carrying out indoor toxicity measurement, wherein the co-toxicity coefficients to the bemisia tabaci 24 hours after the pesticide is applied are respectively 142.72, 124.53, 93.71, 61.19 and 44.72. The results show that: compared with a single agent, the compounded preparation of deltamethrin and guadipyr according to the weight ratio of 9.0-7.0:1.0-3.0 has obvious synergistic effect, wherein the weight ratio of deltamethrin to guadipyr is 9.0: the effect is most prominent at 1.0, and the co-toxicity coefficient to the bemisia tabaci 24 hours after the drug is applied is 142.72. The specific steps and results are detailed in indoor combined toxicological determination.

Indoor combined toxicology determination:

the test agent is a compound formed by 98.5% of deltamethrin technical (produced by Jiangsu Yangxiang agrichemical chemical Co., Ltd.), 96% of Wubichonggua technical (produced by Hefeixiangyu chemical Co., Ltd.) and two technical materials according to weight proportion.

The test method comprises the following steps:

a blade dipping method is adopted. On the basis of a pre-test, a test reagent is dissolved by N, N-dimethylformamide and diluted into 7-8 series concentrations by 0.05 percent of Tween-80 for useDetermination of Single dose LC₅₀(ii) a Deltamethrin and guadipyr are respectively mixed according to the mass ratio of effective components of 9.0:1.0,7.0: 3.0,5.0: 5.0,3.0: 7.0,1.0: 9.0 preparing the medicament compositions with different components, diluting the medicament compositions into 7-8 series concentrations according to the method, and measuring LC of the different medicament compositions₅₀. Beating fresh tobacco leaves into a leaf dish by using a puncher with the diameter of 2.4cm, soaking the leaves in the liquid medicine for 10s, putting the leaves on absorbent paper, airing, clamping the leaves into a glass tube (with the diameter of 2.4cm and the height of 10cm) with 1ml of agar added in advance by using tweezers, and taking 1 piece of the glass tube; sucking the test Bemisia tabaci adults into a pest sucking device, standing for 2 hours, removing dead pests, and moving the healthy Bemisia tabaci adults into a glass tube, wherein 30-40 heads of Bemisia tabaci are repeated each time. Sealing the glass tube by a cotton plug, and then placing the glass tube at the temperature (25 +/-1) DEG C, the relative humidity (70 +/-5)%, the illumination L: d ═ 16: in the illumination incubator of 8, the number of dead insects and the number of live insects of the bemisia tabaci are checked after 24 hours, and the person who can not crawl by touching the insect body with the tip of the writing brush is regarded as dead. Each treatment was repeated 3 times and clear water was used as a control. The control group with the mortality rate below 10% is effective test.

The data processing method comprises the following steps:

calculating respective Process LC Using SPSS₅₀Calculating the co-toxicity coefficient (CTC) of the pharmaceutical composition by the grandsinope method:

co-toxicity coefficient (CTC) ═ mix Actual Toxicity Index (ATI) ÷ mix Theoretical Toxicity Index (TTI) ] x 100

Actual Toxicity Index (ATI) of mixed preparation (LC of standard preparation)₅₀LC of mixing agent₅₀)×100

The Theoretical Toxicity Index (TTI) of the mixture is the Toxicity Index (TI) of the A component multiplied by the content of A in the effective components of the mixture and the Toxicity Index (TI) of the B component multiplied by the content of B in the effective components of the mixture (in the invention, A is deltamethrin, B is guadipyr)

Virulence index (TI) ═ LC of standard agents₅₀LC of test agent₅₀)×100

When the co-toxicity coefficient is obviously more than 100, the synergistic effect is shown; approaching 100, indicating an additive effect; obviously less than 100, indicating antagonism.

Indoor combined virulence determination results:

table 1 shows that deltamethrin and guadipyr are compounded, when the weight ratio of deltamethrin to guadipyr is 9.0-8.0:1.0-2.0, the co-toxicity coefficient (CTC) to bemisia tabaci 24 hours after application is 142.72-124.53, and the compound has obvious synergistic effect, wherein the effect is most prominent at 9.0:1.0, and the co-toxicity coefficient is 142.72; the weight ratio of the two components is 5.0-1.0:5.0-9.0, and the product has antagonistic effect and co-toxicity coefficient (CTC) of 93.71-44.72.

TABLE 1 indoor Combined toxicology assay of deltamethrin E-guadipyr for Bemisia tabaci

According to the result of the indoor combined toxicological determination, the optimal component proportion and the optimal component proportion for preventing and treating the bemisia tabaci are determined as follows:

the preferable component proportion is as follows:

the optimal component ratio is as follows:

the pesticide for preventing and treating bemisia tabaci comprises the following specific preparation steps: weighing the raw materials in proportion, uniformly mixing the raw materials by using a jet mill, and crushing the mixture to obtain the powder with the particle size of 5-10 microns.

The pesticide provided by the invention is used for preventing and controlling bemisia tabaci, and comprises the following specific steps: mixing the pesticide with water, and spraying, wherein the pesticide dosage is 16-20g/667m²The amount of water used was 60kg/667m²。

In conclusion, the invention adopts deltamethrin and guadipyr as main components and is assisted by a composite auxiliary agent, thereby achieving the effects of complementary advantages and complementary effects, obviously improving the pesticide effect, showing obvious synergistic effect and having important significance for effectively preventing and controlling pests such as bemisia tabaci and the like. In addition, the raw materials added by the aid are all converted into products, the yield is high, and three wastes are not generated.

Detailed Description

Example 1:

the pesticide for preventing and treating bemisia tabaci comprises the following raw materials in percentage by weight:

components	Weight (kilogram)
		Deltamethrin	18
Wubichonggua	2
		NNO (2-Naphthalenesulfonic acid formaldehyde Polymer sodium salt)	60
K12 (sodium dodecyl sulfate)	18
		Pull-apart powder (diisobutyl naphthalene sulfonate)	22
Diatomite	880

The specific production operation is as follows: weighing the raw materials in proportion, uniformly mixing the raw materials on a mixer of a jet mill, conveying the raw materials to a storage tank, feeding the raw materials into a bin of the jet mill through a feeder, adjusting working pressure and air pressure, and feeding the mixture into a crushing chamber for crushing. The crushed mixture enters a classifier from a discharge port, and coarse powder returns to a raw material tank through a rotary valve and is crushed together with the raw material. Crushing, feeding into a cyclone separator, collecting in a finished product bin, mixing the finished product and the superfine powder in a double-helix conical mixer, and feeding into a bin on a packaging machine. Sampling (250 g) for analysis: the particle size of the composition is controlled to be 5-10 microns, the deltamethrin is more than or equal to 1.8 percent, the guadipyr is more than or equal to 0.2 percent, the NNO (sodium salt of 2-naphthalenesulfonic acid-formaldehyde polymer) is more than or equal to 6.0 percent, the nekal (sodium diisobutylnaphthalenesulfonate) is more than or equal to 1.8 percent, the K12 (sodium dodecyl sulfate) is more than or equal to 2.2 percent, and the product is qualified.

Deltamethrin-penta-imidacloprid guanidine agricultural chemical insecticide bemisia tabaci test

The test agent is 2% deltamethrin-guadipyr insecticide described in example 1 of the present invention; the control agent is 50% clothianidin water dispersible granule (produced by United pesticide industry Co., Ltd. in Shandong province). The test is carried out on a sunlight greenhouse cucumber plant in the handling place of the Yuhuang temple street in Shandong, Jinan City, Shanxi province, and the cucumber variety to be tested is 3966. The test treatment is 2 percent of deltamethrin-guadipyr per 667m²20g, 16g of clothianidin water dispersible granules with the concentration of 50 percent per 667m²Using 8g as standard control, spraying clear water as blank control, and repeating for 4 times (16 cells) with area of 40m². Spraying the pesticide 1 time in 11 days 6 months in 2018, wherein the pesticide is a knapsack Singapore Linong sprayer. The water consumption is 60kg/667m². The investigation method comprises the following steps: sampling is carried out at 5 points in each cell, 4 cucumbers are fixed at each point, the upper part of each cucumber is marked with 5 leaves, and the number of the live insects of bemisia tabaci on the marked leaves is recorded in each investigation. Calculating the reduction rate of population and correcting the prevention effect.

The data processing method comprises the following steps:

rate of oral cavity decline (%) (number of active insects before and after the drug) ÷ number of oral cavity before and after the drug × 100

Positive control effect (%) - (reduction rate of population in treatment area-reduction rate of population in control area) ÷ (reduction rate of population in 100-control area) × 100

The test results (table 2) show that 20g and 16g of 2% deltamethrin-penta-imidacloprid guanidine in example 1 have better control effect on bemisia tabaci, and the positive control effect of 7d after application is 94.73% and 92.58% respectively, which is obviously better than that of the standard control.

TABLE 2 field control of Bemisia tabaci with the insecticide of example 1

Example 2:

components	Weight (kilogram)
		Deltamethrin	14
Wubichonggua	6
		NNO (2-Naphthalenesulfonic acid formaldehyde Polymer sodium salt)	60
K12 (sodium dodecyl sulfate)	18
		Pull-apart powder (diisobutyl naphthalene sulfonate)	22
Diatomite	880

The specific production operation was the same as in example 1.

The deltamethrin-penta imidacloprid guanidine insecticide obtained in example 2 was used for the test for controlling Bemisia tabaci, the procedure was the same as in example 1, and the test results are shown in Table 3.

Experiment for controlling Bemisia tabaci by deltamethrin-E-Piperidium guanidine agricultural chemical insecticide

The test results (table 3) show that 20g and 16g of 2% deltamethrin-penta-imidacloprid guanidine in example 2 have better control effect on bemisia tabaci, and the positive control effect of 7d after application is 93.77% and 90.86% respectively, which are obviously better than the effect of the standard control.

TABLE 3 field control of Bemisia tabaci with the insecticide of example 2

Claims

1. The pesticide for preventing and controlling bemisia tabaci is characterized by comprising the following components in parts by weight: the raw materials comprise: 20 kg of deltamethrin and guadipyr, 100 kg of auxiliary agent and 880 kg of filler carrier;

the optimal weight ratio of the deltamethrin to the guadipyr is 9.0: 1.0.

the auxiliary agent is a compound auxiliary agent of 2-naphthalene sulfonic acid formaldehyde polymer sodium salt, sodium dodecyl sulfate and nekal powder;

60kg of sodium salt of the 2-naphthalenesulfonic acid-formaldehyde polymer; 18 kg of sodium dodecyl sulfate; 22 kg of nekal powder;

the filler carrier is diatomite.

2. The method for preparing an insecticide for controlling bemisia tabaci as claimed in claim 1, wherein: the method comprises the following specific steps: weighing the raw materials in proportion, uniformly mixing the raw materials by using a jet mill, and crushing the mixture to obtain the powder with the particle size of 5-10 microns.

3. The use method of the pesticide for controlling bemisia tabaci as claimed in claim 1 is characterized by comprising the following specific steps: mixing the pesticide with water, and spraying, wherein the pesticide dosage is 16-20g/667m²The amount of water used was 60kg/667m²。