CN108208015B - Pesticide suspending agent and preparation method and application thereof - Google Patents
Pesticide suspending agent and preparation method and application thereof Download PDFInfo
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- CN108208015B CN108208015B CN201810129843.XA CN201810129843A CN108208015B CN 108208015 B CN108208015 B CN 108208015B CN 201810129843 A CN201810129843 A CN 201810129843A CN 108208015 B CN108208015 B CN 108208015B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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Abstract
The invention provides a pesticide suspending agent which comprises the following components in percentage by mass: 1.0-10.0% of emamectin benzoate, 2000-12000 IU/mg of bacillus thuringiensis, 0.5-10% of synergist, 4.4-43.5% of adjuvant and the balance of deionized water. The pesticide suspending agent adopts the mixing of two reagents with different action mechanisms, namely emamectin benzoate and bacillus thuringiensis, as the main components of the pesticide, and is favorable for delaying the resistance of pests to a single medicament, thereby improving the pesticide effect of the pesticide, prolonging the use period of the pesticide, simultaneously improving the permeability of the pesticide by matching with a synergist, and the synergistic effect is obvious when the two are mixed with each other, thereby being favorable for reducing the application amount of the pesticide and reducing the cost of the pesticide.
Description
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a pesticide suspending agent containing emamectin benzoate and bacillus thuringiensis, and a preparation method and application thereof.
Background
The rice leaf roller is one of the main pests in rice producing areas in China, is widely distributed in each rice area, and is used for hiding and eating the upper epidermis and mesophyll and only leaving white lower epidermis by using larvae to damage rice and wrapping leaves into longitudinal buds; the damage in the seedling stage affects the normal growth of rice and even withers; the damage from the tillering stage to the jointing stage is reduced, the tillering is reduced, the plant is shortened, and the growth period is delayed; after booting, particularly after heading to the period of full heading, the sword-like leaves are damaged, so that flowering and fructification are influenced, the rate of empty shells is improved, and the thousand kernel weight is reduced.
At present, avermectin and chlorpyrifos are mainly used as main medicaments for preventing and controlling cnaphalocrocis medinalis on rice, and frequent and relatively single medicament application easily enhances the resistance of the cnaphalocrocis medinalis to the avermectin and the chlorpyrifos, so that the medicament effect is reduced, the prevention and control cost is increased, and therefore, new alternative medicaments are required in the market for preventing and controlling the cnaphalocrocis medinalis. The pesticide compounding through different action mechanisms is a simple and quick way for solving the problem of pest resistance, and currently, no related report exists on the fact that emamectin benzoate and bacillus thuringiensis are mixed with each other to serve as a main control agent of the rice leaf rollers.
In addition, fall webworms are quarantine pests worldwide, mainly harm ornamental trees of fruit trees and street trees, especially broad-leaved trees, and cause serious harm to crop trees, wood, fruit trees and the like, the first and second generation larvae are mainly harmed and formed into white net curtains at the middle lower parts of crowns, the third generation larvae are harmed and formed into white net curtains at the middle upper parts of crowns, the low-age larvae eat mesophyll in the net curtains, the damaged leaves only leave leaf veins in a white film shape but are withered and yellow, the eating leaves enter a binge eating period after 5 years, are incised and holes, and when the damage is serious, the leaves are eaten by light and then transferred.
At present, single agents such as emamectin benzoate, matrine, bacillus thuringiensis and the like are mainly used for preventing and treating the fall webworms, the prevention and treatment effect is poor, and a compound product of the emamectin benzoate and the bacillus thuringiensis is not reported.
Disclosure of Invention
The invention aims to solve the problems of single component and poor pest control effect of the existing pesticide for controlling rice leaf rollers and fall webworms.
Therefore, the embodiment of the invention provides a pesticide suspending agent which comprises the following components in percentage by mass: 1.0-10.0% of emamectin benzoate, 2000-12000 IU/mg of bacillus thuringiensis, 0.5-10% of synergist, 4.4-43.5% of adjuvant and the balance of deionized water.
Further, the auxiliary agents comprise preservatives, dispersing agents, wetting agents, thickening agents, antifreezing agents and defoaming agents, and the percentage content of each component in the total mass of the pesticide suspending agent is as follows: 0.2-1.0% of preservative, 1.0-10.0% of dispersing agent, 1.0-10.0% of wetting agent, 0.1-2.0% of thickening agent, 1.0-10.0% of antifreezing agent and 0.1-0.5% of defoaming agent.
As one embodiment, the pesticide suspension comprises the following components in percentage by mass: 2.0-8.0% of emamectin benzoate, 4000-12000 IU/mg of bacillus thuringiensis, 0.5-5.0% of azone, 0.3-0.8% of sodium benzoate, 2.0-8.0% of polycarboxylate, 2.0-10.0% of phosphate, 2.0-8.0% of nonylphenol polyoxyethylene ether, 0.2-1.8% of xanthan gum, 1.0-10.0% of ethylene glycol, 0.1-0.5% of organic silicon defoamer and the balance of deionized water.
As one embodiment, the pesticide suspension comprises the following components in percentage by mass: 3.0-7.0% of emamectin benzoate, 5000-10000 IU/mg of bacillus thuringiensis, 1.0-7.0% of thiazone, 0.3-0.6% of sodium benzoate, 4.0-10.0% of polycarboxylate, 1.0-5.0% of sodium lignosulfonate, 3.0-10.0% of alkylphenol polyoxyethylene, 0.2-1.8% of xanthan gum, 1.0-10.0% of propylene glycol, 0.1-0.5% of organic silicon defoamer and the balance of deionized water.
As one embodiment, the pesticide suspension comprises the following components in percentage by mass: 4.0-6.0% of emamectin benzoate, 6000-8000 IU/mg of bacillus thuringiensis, 1.0-10.0% of organic silicon synergist, 0.3-0.5% of sodium benzoate, 5.0-7.0% of polycarboxylate, 2.0-4.0% of sodium dodecyl sulfate, 4.0-5.0% of nonylphenol polyoxyethylene ether, 0.2-0.4% of xanthan gum, 1.0-10.0% of glycerol, 0.1-0.5% of organic silicon defoamer and the balance of deionized water.
As one embodiment, the pesticide suspension comprises the following components in percentage by mass: 1.0-4.0% of emamectin benzoate, 2000-6000 IU/mg of bacillus thuringiensis, 1.0-10.0% of organic silicon synergist, 0.3-0.5% of sodium benzoate, 1.0-4.0% of polycarboxylate, 2.0-4.0% of phosphate, 1.0-5.0% of fatty alcohol-polyoxyethylene ether, 0.2-0.4% of xanthan gum, 1.0-10.0% of ethylene glycol, 0.1-0.5% of organic silicon defoamer and the balance of deionized water.
As one embodiment, the pesticide suspension comprises the following components in percentage by mass: 2.0-6% of emamectin benzoate, 2000-6000 IU/mg of bacillus thuringiensis, 0.5-3.0% of fast T, 0.3-0.5% of sodium benzoate, 1.0-4.0% of polycarboxylate, 2.0-4.0% of phosphate, 2.0-8.0% of nonylphenol polyoxyethylene ether, 0.2-1.8% of xanthan gum, 1.0-10.0% of propylene glycol, 0.1-0.5% of organic silicon defoamer and the balance of deionized water.
As one embodiment, the pesticide suspension comprises the following components in percentage by mass: 2.0 percent of emamectin benzoate, 4000IU/mg of bacillus thuringiensis, 1.0 percent of fast T, 0.4 percent of sodium benzoate, 3.0 percent of polycarboxylate, 3.0 percent of phosphate, 6.0 percent of nonylphenol polyoxyethylene ether, 0.2 percent of xanthan gum, 5.0 percent of propylene glycol, 0.4 percent of organic silicon defoamer and the balance of deionized water.
In addition, the invention also provides a preparation method of the pesticide suspending agent, which comprises the following steps:
1) putting the emamectin benzoate, the bacillus thuringiensis, the synergist and the auxiliary agent into deionized water according to the design amount, and shearing in a high-speed shearing machine for 1h to obtain a mixed solution;
2) pumping the mixed solution obtained in the step 1) into a sand mill through a pneumatic diaphragm pump, serially connecting two sand mills for sanding for 3 hours to enable the particle size of particles in the mixed solution to be less than 5 mu m, pumping the sanded suspension into a shearing homogenizing kettle through the pneumatic diaphragm pump for homogenizing for 1 hour to obtain the pesticide suspending agent.
The pesticide suspending agent provided by the invention is used for preventing and controlling cnaphalocrocis medinalis and fall webworms.
In the invention, the emamectin benzoate can enhance the action of nerve substances such as glutamic acid and gamma-aminobutyric acid (GABA), so that a large amount of chloride ions enter nerve cells, the cell function is lost, nerve conduction is disturbed, larvae stop feeding immediately after contacting, irreversible paralysis occurs, the highest lethality rate is reached within 3-4 days, the activity on cnaphalocrocis medinalis and fall webworm is extremely high, the good effect is achieved at very low dosage, and the insect control process has no harm on beneficial insects, thereby being beneficial to the comprehensive control of the pests, expanding the insecticidal spectrum and reducing the toxicity on human and livestock; the bacillus thuringiensis can generate two major toxins, namely endotoxin (parasporal crystals) and exotoxin, so that pests stop eating the bacillus thuringiensis, and finally the pests die due to hunger and poisoning, so that the bacillus can be used as a microbial source low-toxicity pesticide, and the two components of the emamectin benzoate and the bacillus thuringiensis are different in action mechanism and are mixed with each other for preventing and controlling the pests, and are favorable for delaying the resistance of the pests to a single pesticide, so that the pesticide effect is improved, and the use period of the pesticide is prolonged; meanwhile, the compounded synergist effectively increases the permeability of the emamectin benzoate and the bacillus thuringiensis on the surface of the plant, can reduce the surface tension of the diluent of the suspending agent, reduces the contact angle between the sprayed liquid medicine and the crop, enables the liquid medicine to be easily attached to the surface of the crop leaf, cannot fall to the ground once being stained with the leaf, improves the utilization rate of the medicine, and increases the conduction of the emamectin benzoate and the bacillus thuringiensis in the crop body.
Compared with the prior art, the invention has the beneficial effects that:
(1) the pesticide suspending agent provided by the invention adopts the mixing of two reagents with different action mechanisms, namely emamectin benzoate and bacillus thuringiensis, as the main components of the pesticide, and is beneficial to delaying the resistance of pests to a single medicament, thereby improving the pesticide effect of the pesticide, prolonging the use period of the pesticide, simultaneously being matched with the synergist to improve the permeability of the pesticide, and being obvious in synergistic effect due to the mutual mixing of the emamectin benzoate and the bacillus thuringiensis, and beneficial to reducing the application amount of the pesticide and reducing the cost of the pesticide.
(2) The pesticide suspending agent provided by the invention adopts an environment-friendly aqueous suspending agent form, no dust pollution is caused in the production process, the water-based pesticide has small influence on the environment compared with the production process of powder and granules, and the use is convenient.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
the embodiment provides a pesticide suspending agent, which comprises the following components in percentage by mass: 2.0 percent of emamectin benzoate, 4000IU/mg of bacillus thuringiensis, 1.0 percent of fast T, 0.4 percent of sodium benzoate, 3.0 percent of polycarboxylate, 3.0 percent of phosphate, 6.0 percent of nonylphenol polyoxyethylene ether, 0.2 percent of xanthan gum, 5.0 percent of propylene glycol, 0.4 percent of organic silicon defoamer and the balance of deionized water.
The preparation method of the pesticide suspending agent specifically comprises the following steps:
(1) the preparation method comprises the steps of putting emamectin benzoate, bacillus thuringiensis, fast T, sodium benzoate, polycarboxylate, phosphate ester, nonylphenol polyoxyethylene ether, xanthan gum and propylene glycol into deionized water, and shearing for 1 hour in a high-speed shearing machine to obtain a mixed solution.
(2) Pumping the mixed solution into a horizontal sand mill through a pneumatic diaphragm pump, starting the sand mill, serially connecting two sand mills for sanding for 3 hours to ensure that the particle size of particles in the mixed solution is below 5 mu m, pumping the sanded suspension into a shearing homogenizing kettle through the pneumatic diaphragm pump, and adding an organic silicon defoaming agent for homogenizing for 1 hour to obtain the pesticide suspending agent.
Example 2:
the embodiment provides a pesticide suspending agent, which comprises the following components in percentage by mass: 8.0 percent of emamectin benzoate, 6000IU/mg of bacillus thuringiensis, 5.0 percent of azone, 0.8 percent of sodium benzoate, 8.0 percent of polycarboxylate, 2.0 percent of phosphate, 2.0 percent of nonylphenol polyoxyethylene ether, 0.2 percent of xanthan gum, 8.0 percent of glycol, 0.5 percent of organic silicon defoamer and the balance of deionized water.
The preparation method of the pesticide suspension is the same as that of example 1.
Example 3:
the embodiment provides a pesticide suspending agent, which comprises the following components in percentage by mass: 2.0% of emamectin benzoate, 12000IU/mg of bacillus thuringiensis, 0.5% of azone, 0.3% of sodium benzoate, 2.0% of polycarboxylate, 8.0% of phosphate, 4.0% of nonylphenol polyoxyethylene ether, 1.8% of xanthan gum, 2.0% of ethylene glycol, 0.1% of organic silicon defoamer and the balance of deionized water.
The preparation method of the pesticide suspension is the same as that of example 1.
Example 4:
the embodiment provides a pesticide suspending agent, which comprises the following components in percentage by mass: 7.0 percent of emamectin benzoate, 5000IU/mg of bacillus thuringiensis, 7.0 percent of thioxanthone, 0.6 percent of sodium benzoate, 4.0 percent of polycarboxylate, 5.0 percent of sodium lignosulfonate, 10.0 percent of alkylphenol polyoxyethylene, 1.8 percent of xanthan gum, 10.0 percent of propylene glycol, 0.5 percent of organic silicon defoamer and the balance of deionized water.
The preparation method of the pesticide suspension is the same as that of example 1.
Example 5:
the embodiment provides a pesticide suspending agent, which comprises the following components in percentage by mass: 3.0% of emamectin benzoate, 10000IU/mg of bacillus thuringiensis, 1.0% of thiazone, 0.3% of sodium benzoate, 10.0% of polycarboxylate, 1.0% of sodium lignosulfonate, 3.0% of alkylphenol polyoxyethylene, 0.2% of xanthan gum, 1.0% of propylene glycol, 0.1% of organic silicon defoamer and the balance of deionized water.
The preparation method of the pesticide suspension is the same as that of example 1.
Example 6:
the embodiment provides a pesticide suspending agent, which comprises the following components in percentage by mass: 6.0% of emamectin benzoate, 6000IU/mg of bacillus thuringiensis, 10.0% of organic silicon synergist, 0.5% of sodium benzoate, 7.0% of polycarboxylate, 2.0% of sodium dodecyl sulfate, 4.0% of nonylphenol polyoxyethylene ether, 0.4% of xanthan gum, 1.0% of glycerol, 0.1% of organic silicon defoamer and the balance of deionized water.
The preparation method of the pesticide suspension is the same as that of example 1.
Example 7:
the embodiment provides a pesticide suspending agent, which comprises the following components in percentage by mass: 4.0 percent of emamectin benzoate, 8000IU/mg of bacillus thuringiensis, 1.0 percent of organic silicon synergist, 0.3 percent of sodium benzoate, 5.0 percent of polycarboxylate, 4.0 percent of sodium dodecyl sulfate, 5.0 percent of nonylphenol polyoxyethylene ether, 0.2 percent of xanthan gum, 10.0 percent of glycerol, 0.5 percent of organic silicon defoamer and the balance of deionized water.
The preparation method of the pesticide suspension is the same as that of example 1.
Example 8:
the embodiment provides a pesticide suspending agent, which comprises the following components in percentage by mass: 1.0% of emamectin benzoate, 6000IU/mg of bacillus thuringiensis, 3.0% of organic silicon synergist, 0.5% of sodium benzoate, 4.0% of polycarboxylate, 4.0% of phosphate, 1.0% of fatty alcohol-polyoxyethylene ether, 0.4% of xanthan gum, 1.0% of ethylene glycol, 0.1% of organic silicon defoamer and the balance of deionized water.
The preparation method of the pesticide suspension is the same as that of example 1.
Example 9:
the embodiment provides a pesticide suspending agent, which comprises the following components in percentage by mass: 4.0% of emamectin benzoate, 2000IU/mg of bacillus thuringiensis, 10.0% of organic silicon synergist, 0.3% of sodium benzoate, 1.0% of polycarboxylate, 2.0% of phosphate, 5.0% of fatty alcohol-polyoxyethylene ether, 0.2% of xanthan gum, 10.0% of ethylene glycol, 0.5% of organic silicon defoamer and the balance of deionized water.
The preparation method of the pesticide suspension is the same as that of example 1.
Example 10:
the embodiment provides a pesticide suspending agent, which comprises the following components in percentage by mass: 6.0% of emamectin benzoate, 2000IU/mg of bacillus thuringiensis, 3.0% of fast T, 0.3% of sodium benzoate, 4.0% of polycarboxylate, 4.0% of phosphate, 8.0% of nonylphenol polyoxyethylene ether, 0.2% of xanthan gum, 1.0% of propylene glycol, 0.5% of organic silicon defoamer and the balance of deionized water.
The preparation method of the pesticide suspension is the same as that of example 1.
Example 11:
the embodiment provides a pesticide suspending agent, which comprises the following components in percentage by mass: 2.0% of emamectin benzoate, 6000IU/mg of bacillus thuringiensis, 0.5% of fast T, 0.5% of sodium benzoate, 1.0% of polycarboxylate, 2.0% of phosphate, 2.0% of nonylphenol polyoxyethylene ether, 1.8% of xanthan gum, 10.0% of propylene glycol, 0.1% of organic silicon defoamer and the balance of deionized water.
The preparation method of the pesticide suspension is the same as that of example 1.
Example 12:
in this example, a combined toxicity test of emamectin benzoate and bacillus thuringiensis at different ratios on cnaphalocrocis medinalis and fall webworm is examined, and the specific process is as follows:
1. the instrument comprises the following steps: analytical balance (parts per million), light incubator, pipette, measuring cylinder, tweezers, marker, beaker, volumetric flask, petri dish, feeding bottle, etc
2. Equipment: constant temperature insect-culturing room
3. Test conditions and materials
3.1 biological test Material
The 3 rd instar larvae of the rice leaf roller are collected from the test base of the academy of agricultural sciences of Hubei province, after the larvae are raised for two generations in the test center, healthy 3 rd instar larvae with consistent instar are selected for toxicity measurement. Feeding conditions are as follows: the temperature is (26 +/-2 ℃), the illumination is 14h/d, and the humidity is 85%.
3 rd larva of fall webworm, wherein the larva is collected from Shandong field, and after being collected and raised in an experiment center, the 3 rd larva with consistent age is selected for toxicological determination. The raising conditions include 25 deg.c temperature and 75-80% humidity.
3.2 test Agents
70 percent of emamectin benzoate raw material and 70000IU/mg of bacillus thuringiensis raw material.
4. The test step adopts a leaf soaking method to measure the combined toxicity activity of the medicament
4.1 preparation of test specimens
Selecting fresh and tender rice leaves, cleaning, cutting into 10cm long, and airing in a shade place until no water mark exists on the surface for later use; selecting fresh poplar leaves, cleaning, and airing in a shade until no water mark exists on the surface for later use.
4.2 preparation of the liquid medicine
Dissolving 70% of emamectin benzoate raw material by using a small amount of acetone, and diluting by using an aqueous solution containing 0.1% of T-80 to prepare a 2% mother solution.
70000IU/mg of bacillus thuringiensis raw powder is diluted by sterile water to prepare 4000IU/mg of mother liquor; the two single agents are prepared into mixed mother liquor with different mixture ratios as shown in table 1 by using sterile water, and according to the result of a pre-made experiment, A, B, C, D, E, F, G7 treatments are diluted into 5 mass concentration gradients by using the sterile water: 20. 40, 80, 160, 320mg/L, in accordance with
The leaf method is used for measuring the combined toxicity of the compound medicament.
TABLE 1
4.3 treatment with medicament:
firstly, fresh and tender rice leaves are put into liquid medicine with various treatment concentrations
Taking out and airing for 30s, wrapping the lower parts of the leaves with soaked absorbent cotton, putting the leaves into a glass cup (culture cup), inoculating 12 larvae of the rice leaf roller at 3 rd age into each cup, repeating for 4 times at each concentration for 48 times, and paying attention to protect the humidity of the absorbent cotton wrapping the rice leaves after treatment to prevent the rice leaves from drying up.
② putting fresh poplar leaves into the liquid medicine with each treatment concentration
And (3) taking out and airing for 30s, wrapping petioles with soaked absorbent cotton, putting the petioles into a glass cup (culture cup), inoculating 12 heads of 3-instar larvae of the fall webworm into each cup, repeating for 4 times at each concentration for 48 heads, and after treatment, paying attention to keep the humidity of the absorbent cotton wrapping the poplar leaves and preventing the poplar leaves from drying up.
4.4 feeding and Observation
Putting the treated rice leaf roller test insects into an intelligent illumination incubator (26 +/-1 ℃) with an illumination period L: D ═ 16:8 for feeding and observation.
② the treated fall webworm is put in an intelligent illumination incubator with 25 ℃ and illumination period L: D ═ 16:8 for feeding and observation.
4.5 examination
And checking the test result after 48h, and taking the fact that the brush pen slightly touches the insect body and the larva can not move coordinately as a death standard, and taking the comparison result that the death rate is less than 10 percent as an effective test.
5 data statistics and analysis
5.1 calculation method
Calculating corrected mortality rates for each treatment based on the survey data; calculating according to formulas (1) and (2), and reserving the calculation results to the last two decimal points:
mortality (%) -100% dead/total treated (1),
corrected mortality (%) - (treatment mortality-control mortality)/(1-control mortality) × 100 (2);
the method for measuring the co-toxicity coefficient is carried out according to the method of Sun Yunpei (1960), the co-toxicity coefficient is more than 120, the co-toxicity coefficient shows synergistic effect, the co-toxicity coefficient shows additive effect between 80 and 120, and the co-toxicity coefficient shows antagonistic effect when the co-toxicity coefficient is less than 80.
5.2 statistical analysis
The test data are processed and analyzed by DPS, and the toxicity regression equation, lethal mid-concentration and correlation coefficient R results of each medicament are calculated and shown in tables 2 and 3.
As can be seen from Table 2, 2% emamectin benzoate and 4000IU/mg Bacillus thuringiensis are compounded according to different proportions and have different toxicityThe 1:1 compound has the highest co-toxicity coefficient to 3-instar larvae of rice leaf roller, the CTC value is 124.61, has certain synergistic effect, and LC50Value 68.43. mu.g/ml, LC95The value was 453.50. mu.g/ml, correlation coefficient was 0.9968.
As can be seen from Table 3, the 2% emamectin benzoate and 4000IU/mg bacillus thuringiensis are compounded in different proportions to have different toxicity, the co-toxicity coefficient of the 1:1 compounding to 3 rd larva of fall webworm of poplar is the highest, the CTC value is 136.8786, the synergistic effect is obvious, and LC50Value 56.3028. mu.g/ml, LC95The value was 476.4744. mu.g/ml, correlation coefficient was 0.9921.
5.3 conclusion
Indoor activity determination shows that the compounding of the emamectin benzoate and the bacillus thuringiensis has higher toxicity to 3 rd larvae of rice leaf rollers and 3 rd larvae of poplar hyphantria cunea, 2 percent of the emamectin benzoate and 4000IU/mg of the bacillus thuringiensis have certain synergistic effect by compounding according to a ratio of 1:1 and a ratio of 1:2, wherein the co-toxicity coefficient by compounding according to a ratio of 1:1 is higher.
TABLE 2 statistical analysis of the results of the activity of each treatment reagent on 3 rd instar larvae of cnaphalocrocis medinalis guenee
TABLE 3 statistical analysis of the results of the activity of each treatment reagent on the 3 rd larva of the hyphantria cunea
Example 13:
the example investigates the synergistic effect of the synergist on 2.0% emamectin benzoate and 4000IU/mg bacillus thuringiensis pesticide suspending agent, the specific biological test process is basically the same as the step of the example 12, except that the test agents are 2.0% emamectin benzoate 4000IU/mg bacillus thuringiensis suspending agent, azone, thiazone, organosilicon synergist and fast T; the preparation method of the liquid medicine is to add 2.0 percent of methylamineThe abamectin benzoate 4000IU/mg bacillus thuringiensis suspending agent is prepared into mixed mother liquor with different proportions in the table 4 by compounding a compound preparation with sterile water, and A, B, C, D, E5 treatments are diluted into 5 mass concentration gradients by using the sterile water according to the pre-made experimental result: 10. 20, 40, 80, 160mg/L, in accordance with
The leaf method is used for measuring the combined toxicity of the compound medicament.
TABLE 4 different proportions of emamectin benzoate and Bacillus thuringiensis suspension and synergist
| Treatment of | Medicament |
| A | 2.0% emamectin benzoate 4000IU/mg Bacillus thuringiensis |
| B | 2.0% emamectin benzoate 4000IU/mg Bacillus thuringiensis + 1.5% azone |
| C | 2.0% emamectin benzoate 4000IU/mg Bacillus thuringiensis + 2.0% express T |
| D | 2.0% emamectin benzoate 4000IU/mg Bacillus thuringiensis + 1.5% thioxanthone |
| E | 2.0% emamectin benzoate 4000IU/mg Bacillus thuringiensis +70% of an organosilicon synergist |
Calculating the corrected mortality of each treatment according to the survey data, and calculating according to the following formula, wherein the calculation results are reserved to the last two decimal points;
mortality (%) -100% dead/total treated,
corrected mortality (%) - (treatment mortality-control mortality)/(1-control mortality) × 100
The specific method for determining the virulence coefficient comprises the following steps: the toxicity coefficient of B is LC of B agent by taking A as standard agent50LC of A medicament50(ii) a And when the toxicity coefficient is more than 2.0, the synergistic effect is shown, when the toxicity coefficient is between 1.0 and 2.0, the obvious synergistic effect is not shown, and when the toxicity coefficient is less than 1.0, the antagonistic effect is shown.
The test data are processed and analyzed by DPS, and toxicity regression equation, lethal middle concentration and correlation coefficient R results of each medicament are calculated and shown in tables 5 and 6.
As can be seen from Table 5, the 2.0% emamectin benzoate 4000IU/mg Bacillus thuringiensis suspending agent has different toxicity when being added with different synergists, and the toxicity coefficient of the 2.0% quick T compound added with the suspending agent to 3-year larvae of rice leaf roller is the highest and is 2.88, so that the suspending agent has a synergistic effect, and LC (liquid chromatography) has50The value was 23.52. mu.g/ml, and the correlation coefficient was 0.9995.
As can be seen from Table 6, 2.0% emamectin benzoate 4000IU/mg Bacillus thuringiensis suspending agent has different toxicity when different penetration enhancers are added, and the toxicity coefficient of 2.0% quick T addition compounded on 3 rd larvae of the hyphantria cunea Yang is the highest and is 2.32, so that the synergistic effect is achieved, and LC (liquid chromatography) is used for controlling the growth of the hyphantria cunea Yang50The value was 24.06. mu.g/ml, and the correlation coefficient was 0.9650.
TABLE 5 statistical analysis of the results of the activity of the potentiators on 3 rd instar larvae of cnaphalocrocis medinalis
| Treatment of | Regression equation of virulence | LC50(μg/ml) | R | Coefficient of virulence | Synergistic action |
| A | Y＝2.305+1.4366X | 67.84 | 0.9730 | / | / |
| B | Y＝1.927+1.8799X | 43.04 | 0.9863 | 1.58 | Is free of |
| C | Y＝2.772+1.6250X | 23.52 | 0.9995 | 2.88 | Efficiency enhancement |
| D | Y＝2.705+1.4707X | 36.39 | 0.9912 | 1.86 | Is free of |
| E | Y＝3.093+1.2666X | 32.17 | 0.9922 | 2.11 | Efficiency enhancement |
TABLE 6 statistical analysis of the results of the activity of the potentiators on the 3 rd instar larvae of the hyphantria cunea
| Treatment of | Regression equation of virulence | LC50(μg/ml) | R | Coefficient of virulence | Synergistic action |
| A | Y＝2.825+1.5237X | 55.77 | 0.9949 | / | / |
| B | Y＝2.274+1.6573X | 43.84 | 0.9673 | 1.27 | Is free of |
| C | Y＝3.197+1.3206X | 24.06 | 0.9650 | 2.32 | Efficiency enhancement |
| D | Y＝2.510+1.5595X | 39.51 | 0.9860 | 1.41 | Is free of |
| E | Y＝2.571+1.6637X | 29.55 | 0.9621 | 1.89 | Is free of |
Screening tests were carried out below for the fast T optimum use concentration.
Compounding 2.0% emamectin benzoate 4000IU/mg bacillus thuringiensis suspending agent into mixed mother liquor with different mixture ratios in table 7 by using sterile water, and mixing A, B, C, D, E, F, G7 mixed mother liquor by using sterile water according to the results of pre-made experimentsTreatment dilutions were 5 mass concentration gradients: 10. 20, 40, 80, 160mg/L, in accordance with
The leaf method is used for measuring the combined toxicity of the compound medicament.
TABLE 7 compounding of emamectin benzoate-Bacillus thuringiensis suspension with different concentrations of fast T
The result of this time is statistically calculated according to the synergy test method and the calculation method for testing different synergists in the above examples, and the test results are shown in tables 8 and 9.
TABLE 8 statistical analysis of the results of the activity of each fast concentration T on 3 rd instar larvae of cnaphalocrocis medinalis guenee
| Treatment of | Regression equation of virulence | LC50(μg/ml) | R | Coefficient of virulence | Synergistic action |
| A | Y＝2.111+1.5801X | 66.44 | 0.9862 | / | / |
| B | Y＝2.538+1.4439X | 50.44 | 0.9901 | 1.32 | Is free of |
| C | Y＝3.469+1.0929X | 25.84 | 0.9704 | 2.57 | Efficiency enhancement |
| D | Y＝3.669+0.9800X | 23.85 | 0.9602 | 2.78 | Efficiency enhancement |
| E | Y＝3.525+1.0663X | 24.49 | 0.9859 | 2.71 | Efficiency enhancement |
| F | Y＝3.701+0.9512X | 23.94 | 0.9699 | 2.78 | Efficiency enhancement |
| G | Y＝3.204+1.3022X | 24.63 | 0.9718 | 2.70 | Efficiency enhancement |
TABLE 9 statistical analysis of the results of the activity of each fast concentration T on the 3 rd larvae of the hyphantria cunea Yang
| Treatment of | Regression equation of virulence | LC50(μg/ml) | R | Coefficient of virulence | Synergistic action |
| A | Y＝2.609+1.3731X | 54.99 | 0.9971 | / | / |
| B | Y＝3.130+1.2020X | 36.16 | 0.9696 | 1.52 | Is free of |
| C | Y＝2.950+1.5012X | 24.13 | 0.9629 | 2.29 | Efficiency enhancement |
| D | Y＝3.669+0.9800X | 23.85 | 0.9602 | 2.31 | Efficiency enhancement |
| E | Y＝4.016+0.7253X | 23.77 | 0.9607 | 2.31 | Efficiency enhancement |
| F | Y＝3.770+0.9134X | 23.21 | 0.9628 | 2.37 | Efficiency enhancement |
| G | Y＝3.701+0.9512X | 23.94 | 0.96991 | 2.30 | Efficiency enhancement |
As can be seen from Table 8, the 2.0% emamectin benzoate 4000IU/mg Bacillus thuringiensis suspending agent has different toxicity of quick T when added at different concentrations, but after the addition reaches 1.0%, the subsequent synergistic effect is not obviously improved, which indicates that the synergistic effect can be achieved by adding 1% quick T in the preparation, and the concentration of the quick T is preferably 1%.
As can be seen from Table 9, the 2.0% emamectin benzoate 4000IU/mg Bacillus thuringiensis suspending agent has different toxicity of quick T when added at different concentrations, but after the addition reaches 1.0%, the subsequent synergistic effect is not obviously improved, which indicates that the synergistic effect can be achieved by adding 1% quick T in the preparation, and the concentration of the quick T is preferably 1%.
Example 14:
in this example, a 2.0% emamectin benzoate 4000IU/mg bacillus thuringiensis suspension is used in a field test for controlling cnaphalocrocis medinalis and fall webworms, and the specific process is as follows:
(1) field test for preventing and controlling rice leaf roller
Spraying 2.0% emamectin benzoate (emamectin benzoate for short) 4000IU/mg bacillus thuringiensis suspending agent according to 10 ml/mu to serve as a test group # 1; spraying 2.0% emamectin benzoate 4000IU/mg bacillus thuringiensis suspending agent according to 20 ml/mu to serve as a test group No. 2; spraying 2.0% emamectin benzoate 4000IU/mg bacillus thuringiensis suspending agent according to 40 ml/mu to serve as test group No. 3; spraying 2.0% emamectin benzoate emulsifiable solution according to 30 ml/mu to serve as a test group No. 4; spraying 4000IU/mg of bacillus thuringiensis suspending agent according to 500 ml/mu to serve as a test group No. 5; a blank control was set and the treatment numbers are shown in Table 10.
TABLE 10 test design for test agents
Correspondingly applying different numbered reagents according to a field plot distribution table mode shown in the table 11, wherein the area of each plot is about 20 square meters; the number of repetitions: each treatment was repeated 4 times. The test method adopts a water-adding spraying method, and the test medicament is diluted to various treatment concentrations by tap water and then sprayed by a knapsack electric sprayer. The base number is investigated before pesticide application, five samples are taken in each cell 14 days after pesticide application, 5 clusters of rice (average 15 ears in each cluster of rice) are investigated at each point, 25 clusters of rice are investigated, 2 leaves are counted down in each ear, the total leaf number and the damaged leaf number are recorded, and the leaf rolling rate and the control effect are calculated. The test results are shown in table 12.
TABLE 11 field district distribution table
| 3 | 2 | 5 | 1 | 4 | 6 |
| 1 | 6 | 2 | 3 | 5 | 4 |
| 4 | 3 | 6 | 5 | 1 | 2 |
| 5 | 1 | 4 | 2 | 6 | 3 |
TABLE 12 test results on Cnaphalocrocis medinalis
Note: the control (%) in the table above is the average of each repetition.
The test results in Table 12 show that the 2% emamectin benzoate 4000IU/mg bacillus thuringiensis suspending agent has better control effect on rice leaf rollers, and has a certain synergistic effect compared with two single agents of emamectin benzoate and bacillus thuringiensis.
(2) Field test for preventing and controlling rice leaf roller
Spraying 2.0% emamectin benzoate (emamectin benzoate for short) 4000IU/mg bacillus thuringiensis suspending agent according to 2000-time dilution to obtain test group # 1; spraying 2.0% emamectin benzoate 4000IU/mg bacillus thuringiensis suspending agent according to the dilution of 1500 times to obtain a test group No. 2; spraying 2.0% emamectin benzoate 4000IU/mg bacillus thuringiensis suspending agent by diluting 1000 times to obtain test group No. 3; spraying 2.0% emamectin benzoate emulsifiable concentrate by 1500 times of dilution to obtain test group No. 4; spraying 4000IU/mg of bacillus thuringiensis suspending agent by 300 times of dilution to obtain a test group No. 5; a blank control was set and the treatment numbers are shown in Table 13.
TABLE 13 test design for test agent
Correspondingly applying different numbered reagents according to a field plot distribution table mode shown in a table 14, wherein the area of each plot is 2; the number of repetitions was 4 repetitions per treatment. According to the test, a water-adding spraying method is adopted, the test medicament is diluted by tap water to be each treatment concentration, and then the test medicament is uniformly sprayed on the affected part of the plant by a knapsack electric sprayer. The base number is investigated before the application of the pesticide, one tree is investigated in each cell after 1, 3 and 7 days after the application of the pesticide, one branch damaged in east, west, south, north and middle five different directions of one tree is investigated, the number of insect population is recorded, and the test results are shown in table 15.
TABLE 14 field cell distribution Table
| A | B | C | D |
| CK | D | E | C |
| B | A | B | B |
| D | C | A | A |
| E | CK | CK | E |
| C | E | D | CK |
TABLE 15 test results for fall webworm
| Drug treatment | Control effect 1 day after drug (%) | Control effect 3 days after drug (%) | Control effect after 7 days (%) |
| A | 43.39 | 67.84 | 87.73 |
| B | 45.45 | 70.63 | 88.26 |
| C | 48.25 | 72.92 | 89.50 |
| D | 40.48 | 56.67 | 84.78 |
| E | 43.45 | 68.34 | 88.97 |
Note: the control (%) in the table above is the average of each repetition.
The test results in Table 14 show that the 2% emamectin benzoate 4000IU/mg bacillus thuringiensis suspending agent has better control effect on fall webworms, and has certain synergistic effect compared with two single agents of emamectin benzoate and bacillus thuringiensis.
The above examples are merely illustrative of the present invention and should not be construed as limiting the scope of the invention, which is intended to be covered by the claims and any design similar or equivalent to the scope of the invention.
Claims (4)
1. The pesticide suspending agent is characterized by comprising the following components in percentage by mass: 2.0-6% of emamectin benzoate, 2000-6000 IU/mg of bacillus thuringiensis, 0.5-3.0% of fast T, 0.3-0.5% of sodium benzoate, 1.0-4.0% of polycarboxylate, 2.0-4.0% of phosphate, 2.0-8.0% of nonylphenol polyoxyethylene ether, 0.2-1.8% of xanthan gum, 1.0-10.0% of propylene glycol, 0.1-0.5% of organic silicon defoamer and the balance of deionized water.
2. The pesticide suspension concentrate as defined in claim 1, which comprises the following components in percentage by mass: 2.0 percent of emamectin benzoate, 4000IU/mg of bacillus thuringiensis, 1.0 percent of fast T, 0.4 percent of sodium benzoate, 3.0 percent of polycarboxylate, 3.0 percent of phosphate, 6.0 percent of nonylphenol polyoxyethylene ether, 0.2 percent of xanthan gum, 5.0 percent of propylene glycol, 0.4 percent of organic silicon defoamer and the balance of deionized water.
3. The method for producing a pesticidal suspension agent according to claim 1 or 2, characterized by comprising the steps of:
1) adding the components into deionized water according to the design amount, and shearing in a high-speed shearing machine for 1h to obtain a mixed solution;
2) pumping the mixed solution obtained in the step 1) into a sand mill through a pneumatic diaphragm pump, serially connecting two sand mills for sanding for 3 hours to enable the particle size of particles in the mixed solution to be less than 5 mu m, pumping the sanded suspension into a shearing homogenizing kettle through the pneumatic diaphragm pump for homogenizing for 1 hour to obtain the pesticide suspending agent.
4. The use of a pesticidal suspension concentrate according to claim 1 or 2 for controlling cnaphalocrocis medinalis and fall webworm.
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